CN213266476U - Purification heating device for gene detection sample - Google Patents
Purification heating device for gene detection sample Download PDFInfo
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- CN213266476U CN213266476U CN202021976331.6U CN202021976331U CN213266476U CN 213266476 U CN213266476 U CN 213266476U CN 202021976331 U CN202021976331 U CN 202021976331U CN 213266476 U CN213266476 U CN 213266476U
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 205
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 109
- 238000000746 purification Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 title claims description 18
- 239000002184 metal Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 16
- 230000005855 radiation Effects 0.000 claims description 9
- 230000002068 genetic effect Effects 0.000 abstract 6
- 238000000034 method Methods 0.000 description 3
- 210000002200 mouth mucosa Anatomy 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
本实用新型公开了用于基因检测样本的提纯加热装置，涉及基因检测领域，包括至少一个第一加热件、第一加热室、第二加热件和第二加热室，第一加热件加热作用端作用的基因样本数量少于第二加热件的加热作用端作用的基因样本数量；通过第一加热件和第二加热件的作用对样本盒内的基因样本进行不同模式的加热，在需要对样本盒内的全部基因样本进行加热时，选择第二加热件的加热模式对样本盒内的全部基因样本进行加热处理，当样本盒内只有某一部分的基因样本需要进行加热时，在不用将需要加热处理的样本单独取出的情况下，选择第一加热件的加热模式对样本盒需要加热的基因样本进行加热处理，同时也不会影响其他不需要进行加热处理的基因样本。
The utility model discloses a purification and heating device for genetic testing samples, which relates to the field of genetic testing. The utility model comprises at least one first heating element, a first heating chamber, a second heating element and a second heating chamber. The number of acting gene samples is less than the number of gene samples acting on the heating end of the second heating element; the gene samples in the sample box are heated in different modes through the action of the first heating element and the second heating element. When heating all the genetic samples in the box, select the heating mode of the second heating element to heat all the genetic samples in the sample box. When the processed samples are taken out separately, the heating mode of the first heating element is selected to heat the genetic samples that need to be heated in the sample box, and at the same time, other genetic samples that do not need to be heated will not be affected.
Description
Technical Field
The utility model relates to a gene detection area especially relates to a purification heating device for gene detection sample.
Background
The gene detection is a technology for detecting DNA through blood, other body fluids or cells, which is characterized in that oral mucosa cells or other tissue cells fallen off by a detected person are taken, the gene information of the oral mucosa cells or other tissue cells is amplified, DNA molecular information in the cells of the detected person is detected through specific equipment, the risk of diseases of the body is predicted, and the conditions of various genes contained in the oral mucosa cells or other tissue cells are analyzed, so that people can know the gene information of the people, the occurrence of the diseases is avoided or delayed by improving the living environment and living habits of the people, high-temperature heat treatment is an essential flow in the gene detection, a system and an application method for nucleic acid purification, amplification and gene detection are disclosed in the prior art (the Chinese patent application number is CN201710823869.X), a heating part maintains the temperature of a sample area to be detected to be the required temperature, and all detection samples in the detection sample area are heated, but the heating can not be carried out on part of the samples, and the requirement of the detection experiment can not be met.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the problems and design a purification heating device for gene detection samples.
The utility model discloses a following technical scheme realizes above-mentioned purpose:
a purification heating device for gene detection samples, which is used for heating gene samples in a sample box, and comprises:
at least one first heating element; the heating action end of the first heating element acts on the gene sample with the same heating requirement in the sample box;
a first heating chamber; the first heating element is arranged in the first heating chamber;
a second heating member; the heating action ends of the second heating elements act on all the gene samples in the sample box, and the number of the gene samples acted by the heating action ends of the first heating elements is less than that of the gene samples acted by the heating action ends of the second heating elements;
a second heating chamber; the second heating member is installed in the second heating chamber, and when carrying out the gene sample heating of the heating demand of the same race, the sample box is arranged in first heating chamber, and when carrying out the heating of whole gene samples, the second heating chamber is arranged in to the sample box.
The beneficial effects of the utility model reside in that: the gene samples in the sample box are heated in different modes through the action of the first heating element and the second heating element, when all the gene samples in the sample box need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box, when only a certain part of the gene samples in the sample box need to be heated, under the condition that the samples needing to be heated do not need to be taken out independently, the heating mode of the first heating element is selected to heat the gene samples needing to be heated in the sample box, and meanwhile, other gene samples which do not need to be heated are not influenced.
Drawings
FIG. 1 is a schematic view of a heating apparatus for gene assaying sample purification 1;
FIG. 2 is a schematic view of the structure of a heating apparatus for purification of a sample for gene assay 2;
FIG. 3 is a schematic view of the structure of a heating apparatus for purification of a gene assaying sample 3;
FIG. 4 is a schematic view showing the structure of a sample cassette of a heating apparatus for purification of a gene assaying sample;
wherein corresponding reference numerals are:
1-a sample box, 2-a heating groove, 3-a first heating chamber, 4-a second heating chamber, 5-a metal block, 6-a boss, 7-a placing table, 8-a through groove, 9-a heating sheet and 10-a heating base.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "inner", "outer", "left", "right", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are merely for convenience of description of the present invention and simplifying the description, but do not indicate or imply that the device or element that is referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed" and "connected" are to be interpreted broadly, and for example, "connected" may be a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the connection may be direct or indirect via an intermediate medium, and may be a communication between the two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
The following describes in detail embodiments of the present invention with reference to the accompanying drawings.
Example 1, as shown in fig. 1 and 2,
a purification heating device for gene detection samples, which is used for heating gene samples in a sample box 1, comprises:
at least one first heating element; the heating action end of the first heating element acts on the gene samples with the same heating requirements in the sample box 1;
a first heating chamber 3; the first heating member is installed in the first heating chamber 3;
a second heating member; the heating action ends of the second heating elements act on all the gene samples in the sample box 1, and the number of the gene samples acted by the heating action ends of the first heating elements is less than that of the gene samples acted by the heating action ends of the second heating elements;
a second heating chamber 4; the second heating member is installed in the second heating chamber 4.
When all the gene samples in the sample box 1 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1, and when only a certain part of the gene samples in the sample box 1 need to be heated, the heating mode of the first heating element is selected to heat the gene samples needing to be heated in the sample box 1 under the condition that the samples needing to be heated are not taken out separately, and meanwhile, other gene samples which do not need to be heated are not influenced.
Example 2, as shown in fig. 1 and 2,
this example differs from example 1 in that: the second heating element is a heating base 10, a heating sheet 9 is arranged in the heating base 10, and the upper surface of the heating base 10 is in contact with the bottom of the sample box 1 to heat all the gene samples in the sample box 1.
When all the gene samples in the sample box 1 need to be heated, the bottom of the sample box 1 is placed on the upper surface of the heating base 10, and the upper surface of the heating base 10 is contacted with the bottom of the sample box 1 to heat all the gene samples in the sample box 1; when only a certain part of the gene sample in the sample box 1 needs to be heated, the gene sample needing to be heated in the sample box 1 is heated by selecting the heating mode of the first heating element under the condition that the sample needing to be heated is not taken out separately, and other gene samples needing not to be heated are not influenced.
Example 3, as shown in figures 1 and 2,
this example differs from example 2 in that: the upper surface of the heating base 10 is provided with a plurality of heating grooves 2, the bottom of each sample placing position of the sample box 1 protrudes outwards to form a boss 6, and one heating groove 2 contacts with the boss 6 at the bottom of one sample placing position to heat the gene sample in the sample placing position.
When all gene samples in the sample box 1 need to be heated, the boss 6 at the bottom of each sample placing position of the sample box 1 is in contact with the heating groove 2 on the heating base 10, so that the contact area between the bottom of the sample placing position and the heating base 10 is increased, and the heating efficiency of the heating base 10 on the gene samples is accelerated.
Example 4, as shown in figures 1 and 2,
this example differs from example 3 in that: the heating groove 2 on the heating base 10 and the boss 6 at the bottom of the sample placing position are both hemispherical.
The heating groove 2 on the heating base 10 and the boss 6 at the bottom of the sample placing position are both hemispherical, so that the contact area between the bottom of the sample placing position and the heating base 10 is further increased, and the heating efficiency of the heating base 10 on the gene sample is further accelerated.
Example 5. as shown in figure 4,
this example differs from example 1 in that: the second heating member is liquid and heating plate 9, and liquid holds in second heating chamber 4, and heating plate 9 is used for the heating liquid, carries out all gene sample heating time, and sample box 1 is located second heating chamber 4, and liquid parcel is at sample box 1 lateral part and bottom, and the highest height of liquid is slightly less than the lateral wall height of sample box 1, and liquid is water.
When all the gene samples in the sample box 1 need to be heated, the sample box 1 is placed in the liquid in the second heating chamber 4, so that the liquid is wrapped at the side and the bottom of the sample box 1, the highest height of the liquid is slightly lower than the height of the side wall of the sample box 1, and the liquid is heated by the heating sheet 9 through the heating liquid, so that the gene samples in the sample box 1 are subjected to heating treatment; when only a certain part of the gene sample in the sample box 1 needs to be heated, the gene sample needing to be heated in the sample box 1 is heated by selecting the heating mode of the first heating element under the condition that the sample needing to be heated is not taken out separately, and other gene samples needing not to be heated are not influenced.
Example 6. as shown in figure 1,
this example differs from any of examples 1-5 in that: the first heating part comprises a metal block 5, a heating rod is arranged in the metal block 5, the upper surface of the metal block 5 is in contact with the bottom of a sample placing position needing to be heated in the sample box 1 to heat the gene sample in the sample placing position, and the sample placing positions of the sample box 1 are all in a rectangular array in the sample box 1.
When all the gene samples in the sample box 1 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1; the gene samples in the same row of sample positions are used as a group, the conditions of all the processes when the gene samples in the same group are tested are completely the same, the influence of the exceptional conditions on the final result is avoided, however, since some conditions of different groups of gene samples need to be different during experimental detection, when heat treatment is performed, it may be necessary to heat only one or more gene samples in the sample cassette 1, when the heating pattern of the first heating member is selected to heat-treat the gene sample to be heated in the sample cartridge 1 without taking out the sample to be heat-treated separately, the sample box 1 is placed in the first heating chamber 3, the bottoms of the sample placement positions of the row of gene samples needing to be heated are in contact with the upper surface of the metal block 5, and the gene samples in the row of sample placement positions are heated, and meanwhile, other gene samples which do not need to be heated are not influenced.
Example 7. as shown in figure 1,
this example differs from example 6 in that: the upper surface of the metal block 5 is provided with a plurality of heating grooves 2, the bottom of each sample placing position of the sample box 1 protrudes outwards to form a boss 6, and one heating groove 2 is contacted with the boss 6 at the bottom of one sample placing position to heat the gene sample in the sample placing position.
The boss 6 at the bottom of the sample position of the line of the sample box 1 is contacted with the heating groove 2 on the metal block 5, so that the contact area between the boss 6 at the bottom of the sample position of the line and the metal block 5 is increased, and the heating efficiency of the metal block 5 on the gene sample is accelerated.
Example 8. as shown in figure 1,
this example differs from example 7 in that: the heating groove 2 on the metal block 5 and the boss 6 at the bottom of the sample placement position are both hemispherical.
The heating groove 2 on the metal block 5 and the boss 6 at the bottom of the sample placement position are both hemispherical, so that the contact area between the boss 6 at the bottom of the sample placement position and the metal block 5 is further increased, and the heating efficiency of the metal block 5 on the gene sample is further accelerated.
Example 9 the present inventors have found that, as shown in figure 2,
this example differs from any of examples 1-5 in that: first heating member is including placing platform 7 and thermal radiation infrared light source, places and sets up at least one logical groove 8 on the platform 7, and the one end that leads to groove 8 is placed in to the appearance position of putting that needs the heating in the sample box, and the other end that leads to groove 8 is placed in to thermal radiation infrared light source, and thermal radiation infrared light source passes through logical groove 8 and heats this gene sample of putting in the appearance position.
When all the gene samples in the sample box 1 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1; the gene samples in the same row of sample positions are used as a group, the conditions of all the processes when the gene samples in the same group are subjected to experimental detection are completely the same, the influence on the final result caused by exceptional conditions is avoided, but certain conditions of the gene samples in different groups need to be different when the experimental detection is carried out, so that only one group or a plurality of groups of gene samples in the sample box 1 need to be subjected to heating treatment when the heating treatment is carried out, under the condition that the samples needing to be subjected to the heating treatment are not taken out independently, the sample box 1 is placed in the first heating chamber 3 when the heating mode of the first heating element is selected to carry out the heating treatment on the gene samples needing to be heated in the sample box 1, the bottoms of the sample positions of the row of the gene samples needing to be heated are placed right above the through grooves 8 of the placing table 7, the thermal radiation infrared light source is placed right below the through grooves 8 of the placing table 7, the infrared light that thermal radiation infrared light source produced is parallel with logical groove 8, and thermal radiation infrared light source passes logical groove 8 and heats the gene sample in this row's position of putting appearance, also can not influence other gene samples that need not carry out heat treatment simultaneously.
The technical scheme of the utility model is not limited to the restriction of above-mentioned specific embodiment, all according to the utility model discloses a technical scheme makes technical deformation, all falls into within the protection scope of the utility model.
Claims (9)
1. A purification heating device for gene detection sample, which is used for heating gene sample in a sample box, and is characterized by comprising:
at least one first heating element; the heating action end of the first heating element acts on the gene sample with the same heating requirement in the sample box;
a first heating chamber; the first heating element is arranged in the first heating chamber;
a second heating member; the heating action ends of the second heating elements act on all the gene samples in the sample box, and the number of the gene samples acted by the heating action ends of the first heating elements is less than that of the gene samples acted by the heating action ends of the second heating elements;
a second heating chamber; the second heating member is installed in the second heating chamber, and when carrying out the gene sample heating of the heating demand of the same race, the sample box is arranged in first heating chamber, and when carrying out the heating of whole gene samples, the second heating chamber is arranged in to the sample box.
2. The heating apparatus for gene testing sample purification according to claim 1, wherein the second heating member is a heating base, a heating plate is installed inside the heating base, and when heating of the whole gene sample is performed, the upper surface of the heating base contacts with the bottom of the sample box to heat the whole gene sample in the sample box.
3. The heating apparatus for purifying gene testing samples as claimed in claim 2, wherein the upper surface of the heating base is provided with a plurality of heating grooves, the bottom of each sample placing position of the sample box protrudes outwards to form a boss, and one heating groove contacts with the boss at the bottom of one sample placing position to heat the gene sample in the sample placing position.
4. The heating apparatus for purifying gene testing samples according to claim 3, wherein the heating groove on the heating base and the boss at the bottom of the sample placement position are both hemispherical.
5. The purification heating device for the gene detection sample as claimed in claim 1, wherein the second heating element is a liquid and a heating sheet, the liquid is contained in the second heating chamber, the heating sheet is used for heating the liquid, when the whole gene sample is heated, the sample box is located in the second heating chamber, the liquid is wrapped at the side and the bottom of the sample box, and the highest height of the liquid is slightly lower than the height of the side wall of the sample box.
6. The heating apparatus for purifying a gene test sample according to any one of claims 1 to 5, wherein the first heating member comprises a metal block, a heating rod is installed in the metal block, and when the gene sample requiring the same kind of heating is heated, the upper surface of the metal block is contacted with the bottom of the sample position requiring heating in the sample box to heat the gene sample in the sample position.
7. The heating apparatus for purifying gene assaying samples as claimed in claim 6, wherein the metal block has a plurality of heating grooves on its upper surface, the bottom of each sample-placing position of the sample cassette protrudes outward to form a projection, and one heating groove contacts the projection at the bottom of one sample-placing position to heat the gene sample in the sample-placing position.
8. The heating apparatus for purifying gene assaying samples as claimed in claim 7, wherein the heating groove on the metal block and the boss at the bottom of the sample placement site are both hemispherical.
9. The purification heating device for gene test samples according to any one of claims 1 to 5, wherein the first heating member comprises a placing table and a thermal radiation infrared light source, the placing table is provided with at least one through groove, when gene samples with the same heating requirements are heated, a sample placing position to be heated in the sample box is placed at one end of the through groove, the thermal radiation infrared light source is placed at the other end of the through groove, and the thermal radiation infrared light source penetrates through the through groove to heat the gene samples in the sample placing position.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021976331.6U CN213266476U (en) | 2020-09-11 | 2020-09-11 | Purification heating device for gene detection sample |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021976331.6U CN213266476U (en) | 2020-09-11 | 2020-09-11 | Purification heating device for gene detection sample |
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| CN213266476U true CN213266476U (en) | 2021-05-25 |
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| CN202021976331.6U Active CN213266476U (en) | 2020-09-11 | 2020-09-11 | Purification heating device for gene detection sample |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Purification and heating device for genetic testing samples Granted publication date: 20210525 Pledgee: Chenghua sub branch of Bank of Chengdu Co.,Ltd. Pledgor: Chengdu Hanchen Guangyi Bioengineering Co.,Ltd. Registration number: Y2024510000216 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |