CN212610622U

CN212610622U - Bearing device and sequencing system

Info

Publication number: CN212610622U
Application number: CN201821513816.4U
Authority: CN
Inventors: 郑焦; 张松振; 姜泽飞; 周志良; 颜钦
Original assignee: Genemind Biosciences Co Ltd
Current assignee: Genemind Biosciences Co Ltd
Priority date: 2018-09-13
Filing date: 2018-09-13
Publication date: 2021-02-26
Anticipated expiration: 2028-09-13

Abstract

The utility model discloses a bear device and sequencing system bears the device and includes base and connecting portion, connecting portion with the base pivot is connected, the upper surface of base is equipped with the standing groove that is used for placing the reactor, the standing groove include first side and with the second side that first side is adjacent, first side is provided with first location structure, the second side is equipped with second location structure. Above-mentioned load-bearing device sets up location structure respectively through first side and second side adjacent for when the reactor was placed at the standing groove, location structure can carry out prepositioning to the reactor better, guarantees the establishment of flow path.

Description

Bearing device and sequencing system

Technical Field

The utility model relates to a gene sequencing technical field, in particular to bear device and a sequencing system.

Background

With the continuous development of nucleic acid sequencing technology, sequencing systems are also continuously updated. For nucleic acid molecules to be detected located in a reactor, such as a chip, a sequencing system utilizes a detection chip to determine the base sequence of the nucleic acid molecule.

Generally, the chip has a small size, the amount of the reaction reagent connected to the chip is micro/small, and the flow tube such as a pipe or a through hole which is arranged at a specific position and is communicated with the chip is narrow, and when the chip is placed in a sequencing system, the chip needs to be accurately positioned to the specific position so as to be well communicated with the relevant flow tube, so that the sequencing reaction can be realized in the chip placed in the sequencing system.

Therefore, in general, the sequencing system includes a carrier for positioning the chip. How to design or modify the structure/apparatus to ensure that the chip placed in the sequencing system is actually located at the specific position before sequencing becomes a problem to be solved.

SUMMERY OF THE UTILITY MODEL

The utility model provides a bear device and sequencing system.

The utility model discloses embodiment's a bear device, including base and connecting portion, connecting portion with the base pivot is connected, the upper surface of base is equipped with the standing groove that is used for placing the reactor, the standing groove include first side and with the adjacent second side in first side, first side is provided with first location structure, the second side is equipped with second location structure.

The utility model discloses a bear device sets up location structure respectively through first side and the second side adjacent for when the standing groove was placed to the reactor, location structure can carry out prepositioning to the reactor better, guarantees the establishment of flow path, so that the sequencing reaction can go on.

In some embodiments, the placement slot includes a third side adjacent to the first side and a fourth side opposite to the second side, the fourth side connecting the third side and the second side, and the fourth side opposite to the first side, and the carrier includes a side-pushing mechanism at the junction of the third side and the fourth side, the side-pushing mechanism being telescopically disposed in the placement slot and adapted to secure the reactor against the first positioning structure and the second positioning structure.

In some embodiments, the first and second locating structures comprise locating posts, or one of the first and second locating structures comprises locating posts and the other of the first and second locating structures comprises locating slots.

In some embodiments, the number of the positioning columns located on the same side is at least two.

In some embodiments, the third side and the second side are provided with a flow tube, and the flow tube is provided with a flow channel which is used for communicating with the reactor.

In some embodiments, the base is provided with a rotating shaft seat at the outer side of the placing groove;

the connecting portion comprises two clamping edges and a connecting edge, one ends of the two clamping edges are respectively connected with two ends of the connecting edge, and the other ends of the two clamping edges are connected with the rotating shaft seat rotating shaft.

In some embodiments, the connecting edge is provided with a first lock catch, and the base is provided with a second lock catch and an unlocking button. The first lock catch is used for being inserted into the second lock catch or the second lock catch is used for being inserted into the first lock catch so as to lock the connecting part and the base. The unlocking button is used for unlocking the first lock catch and the second lock catch to lock the connecting part and the base.

In some embodiments, the surface of the clamping edge facing the base is provided with a pressing plate for pressing the reactor on the holding tank.

In some embodiments, a torsion spring is disposed at a connection position of the clamping edge and the rotating shaft seat, and one end of the torsion spring abuts against the clamping edge and the other end of the torsion spring abuts against the rotating shaft seat, so that the connecting portion is kept in an open state.

In certain embodiments, the carrier means comprises an elastic member to which the flow stream tube is connected to enable the flow stream tube to elastically abut the reactor.

The utility model discloses sequencing system of embodiment, including the device that bears of any one above-mentioned embodiment.

The utility model discloses a sequencing system sets up location structure respectively through first side and the second side adjacent to for when the standing groove was placed to the reactor, location structure can carry out prepositioning to the reactor better, guarantees the establishment of flow path, so that the sequencing reaction can go on.

Additional aspects and advantages of embodiments of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

fig. 1 is a schematic perspective view of a carrying device according to an embodiment of the present invention;

fig. 2 is another perspective view of the carrying device according to the embodiment of the present invention;

fig. 3 is a top view of a base of an embodiment of the present invention;

fig. 4 is a schematic perspective view of a sequencing system according to an embodiment of the present invention;

fig. 5 is another perspective view of a sequencing system according to an embodiment of the present invention.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the description of the present invention, unless otherwise expressly specified or limited, the first feature "on" or "under" the second feature may include both the first and second features being in direct contact, and may also include the first and second features being in contact, not being in direct contact, but rather being in contact with each other via additional features between them. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The following disclosure provides many different embodiments, or examples, for implementing different features of the invention. In order to simplify the disclosure of the present invention, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. Furthermore, the present invention may repeat reference numerals and/or reference letters in the various examples, which have been repeated for purposes of simplicity and clarity and do not in themselves dictate a relationship between the various embodiments and/or arrangements discussed. In addition, the present disclosure provides examples of various specific processes and materials, but one of ordinary skill in the art may recognize applications of other processes and/or use of other materials.

Referring to fig. 1-3, a carrying device 100 according to an embodiment of the present invention includes a base 102 and a connecting portion 104, the connecting portion 104 is pivotally connected to the base 102, a placing slot 106 for placing a reactor 200 is disposed on an upper surface of the base 102, the placing slot 106 includes a first side 108 and a second side 110 adjacent to the first side 108, the first side 108 is disposed with a first positioning structure 112, and the second side 110 is disposed with a second positioning structure 114.

In the carrying device 100, the positioning structures are respectively disposed on the adjacent first side 108 and the second side 110, so that when the reactor 200 is placed in the placing groove 106, the positioning structures can pre-position the reactor 200 well, and the establishment of a flow path is ensured, so that a sequencing reaction can be performed.

Specifically, when the carrier 100 is applied to a sequencing system, the carrier 100 is placed with the reactor 200, and the sequencing system will introduce reagents for reaction or washing or imaging into the carrier 100. The reactor 200 is provided with a reactor channel (channel) for carrying the reagents, and the flow path of the sequencing system itself needs to be connected to the reactor channel of the reactor 200, so that the pre-positioning and fixing of the reactor 200 are particularly important. Through the positioning structure, when the reactor 200 is placed in the placing groove 106, the reactor can be pre-positioned first, so that the subsequent fixation is facilitated. The reactor 200 is, for example, a chip.

In some embodiments, the placement slot 106 includes a third side 116 and a fourth side 118, the third side 116 is adjacent to the first side 108, the third side 116 is opposite to the second side 110, the fourth side 118 connects the third side 116 to the second side 110, the fourth side 118 is opposite to the first side 108, the carrier 100 includes a side pushing mechanism 120 at the connection of the third side 116 and the fourth side 118, the side pushing mechanism 120 is telescopically disposed in the placement slot 106 and is used for securing the reactor 200 against the first positioning structure 112 and the second positioning structure 114.

Thus, the side pushing mechanism 120 can be matched with the waist hole of the reactor 200, so that over-constraint in the placement groove 106 caused by the placement of the reactor 200 is avoided, and meanwhile, the side pushing mechanism ensures that the reactor 200 is tightly attached to a positioning structure, so that positioning and fixing are realized.

Specifically, in the embodiment of fig. 3, the planar shape of the placement slot 106 is substantially square, the first side 108 is an upper side, the second side 110 is a right side, the third side 116 is a left side, the fourth side 118 is a lower side, and the side pushing mechanism 120 is provided at a junction of the left side and the lower side.

The side pushing mechanism 120 includes a side pushing member 122 and a first elastic member (not shown), the first elastic member is disposed in the base 102, the side pushing member 122 is connected to the first elastic member and partially protrudes into the placing groove 106, so that when the reactor 200 is placed in the placing groove 106, the first elastic member can apply an elastic force to the reactor 200 through the side pushing member 122, and the reactor 200 is tightly attached to the positioning structures on the upper side and the right side.

It is understood that the first side 108, the second side 110, the third side 116, and the fourth side 118 may be other sides than those shown in the above figures. The specific arrangement side can be set according to the position of the waist hole formed in the reactor 200. In one example, the first elastic member may be a spring.

In some embodiments, the first and second locating

structures

112 and 114 include locating posts 124. In this manner, the positioning post 124 can mate with a hole (e.g., the waist hole) of the reactor 200 to pre-position the reactor 200.

Specifically, the number of the positioning pillars 124 located on the same side is at least two. In the illustrated embodiment, the first positioning structure 112 on the first side 108 includes two positioning posts 124, and the second positioning structure 114 on the second side 110 includes one positioning post 124, such that a three-point positioning of the reactor 200 is achieved. Two positioning posts 124 on the first side 108 may be disposed in a direction parallel to the reactor channel. The positioning column can be a positioning pin.

It is understood that in other embodiments, the second positioning structure on the second side 110 may include two positioning posts, and the first positioning structure on the first side 108 may include one positioning post. The two positioning pillars on the second side 110 can be disposed along a direction perpendicular to the reactor channel.

In other embodiments, one of the first and second locating structures includes a locating post and the other of the first and second locating structures includes a locating slot. In this manner, pre-positioning to the reactor 200 may also be achieved, and different configurations of the reactor 200 may be matched.

In some embodiments, the third side 116 and the second side 110 are each provided with a flow channel 126, the flow channel 126 is provided with a flow channel 128, and the flow channel 126 is used for communicating with the reactor 200.

In this way, the establishment of the flow paths of the reactor channels and the sequencing system is facilitated.

Specifically, in the illustrated embodiment, the third side 116 is the left side and is the outlet side of the reactor 200, and the flow stream tube 126 located on the third side 116 is the outlet stream tube. The second side 110 is the right side, the inlet side of the reactor 200, and the flow stream tubes 126 located on the second side 110 are inlet flow tubes.

In one example, the reactor 200 is provided with 16 reactor channels, the inlet flow tube is provided with 2 inlet channels of 1 in 8, the inlet of one inlet channel is connected with one outlet of the three-way valve positioned at the upstream, the inlet of the other inlet channel is connected with the other outlet of the three-way valve positioned at the upstream, and the inlet of the three-way valve is connected with the rotary valve. The outlet flow tube is provided with 16 outlet flow channels. The motive device may be connected to the outlet flow tube such that the motive device may drive the flow of liquids within the flow path of the sequencing system and within the reactor channel.

It will be appreciated that the number of reactor channels, inlet flow channels and outlet flow channels of the above examples are illustrated as examples only and should not be construed as limiting the invention.

In some embodiments, the carrier 100 comprises a second resilient member (not shown) to which the flow stream tube 126 is connected to enable the flow stream tube to resiliently abut the reactor 200. Thus, when the reactor 200 is pressed against the flow channel flow tube, the connection between the reactor 200 and the flow channel flow tube 126 is elastic, and damage to the two is avoided. In one example, the second elastic member may be a spring. In the illustrated example, the second resilient member is disposed below the flow path flow tube 126.

In some embodiments, the base 102 is provided with a rotating shaft seat 130 at the outer side of the placement groove 106;

the connecting portion 104 includes two clamping edges 132 and a connecting edge 134, one end of each of the two clamping edges 132 is connected to the two ends of the connecting edge 134, and the other end of each of the two clamping edges 132 is connected to the rotating shaft seat 130. In this manner, the rotatable connection of the base 102 and the connecting portion 104 may be achieved.

Specifically, the connecting edge 134 is provided with a first locking buckle 136, and the base 102 is provided with a second locking buckle 138 and an unlocking button 140;

the first lock catch 136 is inserted into the second lock catch 138 to lock the connecting portion 104 and the base 102;

the unlocking button 140 is used for unlocking the second lock 138 to lock the connecting portion 104 and the base 102.

When the connection portion 104 and the base 102 are locked, the placement groove 106 can be in a non-open state, which is a state in which the reactor 200 cannot be taken out or put in.

In addition, the surface of the clamping edge 132 facing the susceptor 102 is provided with a pressing plate 142, and the pressing plate 142 is used for pressing the reactor 200 in the placing groove 106. This may allow the reactor 200 to be more securely placed in the holding tank 106.

A torsion spring (not shown) is disposed at a connection portion between the clamping edge 132 and the rotating shaft seat 130, and one end of the torsion spring abuts against the clamping edge 132 and the other end abuts against the rotating shaft seat 130, so that the connecting portion 104 is kept in an open state.

In other embodiments, the second lock catch is configured to be inserted into the first lock catch to lock the connecting portion 104 with the base 102.

Referring to fig. 4 and fig. 5, a sequencing system 300 according to an embodiment of the present invention includes the carrying device 100 according to any of the above embodiments.

The utility model discloses a sequencing system 300 through set up location structure respectively at adjacent first side 108 and second side 110 for reactor 200 places when standing groove 106, and location structure can carry out prepositioning to reactor 200 better, guarantees the establishment of flow path, so that the sequencing reaction can go on.

Specifically, the sequencing system 300 includes a reagent cartridge 144 for storing reagents, and a rotary valve, a three-way valve, the carrying device 100 and the power device 146 are sequentially arranged in the flowing direction of the liquid flowing out from the reagent cartridge 144. The reactor 200 is placed in the carrier 100 and the reactor channels are connected to the flow path of the sequencing system 300. Different reagents can be introduced into the flow path by rotating the valve to perform different reactions within the reactor channel including, but not limited to, extension, excision, capping, imaging, washing, and the like.

The motive device 146 may employ a pump 148, and the pump 148 may provide motive force to the liquid in the flow path.

In other embodiments, the sequencing system may omit the three-way valve and the inlet flow line 112 connects the rotary valve to the reactor 200.

In the description herein, references to the description of the terms "one embodiment," "certain embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. The bearing device is characterized by comprising a base and a connecting part, wherein the connecting part is connected with a rotating shaft of the base, a placing groove for placing a reactor is arranged on the upper surface of the base, the placing groove comprises a first side and a second side adjacent to the first side, a first positioning structure is arranged on the first side, and a second positioning structure is arranged on the second side.

2. The carrier according to claim 1 wherein the placement channel includes a third side adjacent to the first side opposite the second side and a fourth side connecting the third side to the second side opposite the first side, the carrier including a side-pushing mechanism at the junction of the third side and the fourth side, the side-pushing mechanism being telescopically disposed in the placement channel and adapted to secure the reactor against the first and second positioning structures.

3. The carrying device as claimed in claim 1 or 2, wherein the first and second positioning structures comprise positioning posts, or

One of the first positioning structure and the second positioning structure comprises a positioning column, and the other of the first positioning structure and the second positioning structure comprises a positioning groove.

4. The carrying device as claimed in claim 3, wherein the number of positioning pillars located on the same side is at least two.

5. The carrier device as claimed in claim 2, wherein the third side and the second side are each provided with a flow channel, the flow channels being provided with flow channels for communicating with the reactor.

6. The carrying device according to claim 1 or 2, wherein the base is provided with a rotating shaft seat at the outer side of the placing groove;

7. The carrying device as claimed in claim 6, wherein the connecting edge is provided with a first locking buckle, and the base is provided with a second locking buckle and an unlocking button;

the first lock catch is used for being inserted into the second lock catch or the second lock catch is used for being inserted into the first lock catch so as to lock the connecting part and the base;

the unlocking button is used for unlocking the first lock catch and the second lock catch to lock the connecting part and the base.

8. The carrier according to claim 6, wherein the surface of the clamping edge facing the base is provided with a pressing plate for pressing against the reactor in the holding tank.

9. The carrying device as claimed in claim 6, wherein a torsion spring is provided at the connection between the clamping edge and the rotating shaft seat, and one end of the torsion spring abuts against the clamping edge and the other end abuts against the rotating shaft seat, so that the connecting part is kept in an opened state.

10. The carrier device of claim 5, wherein the carrier device comprises an elastic member to which the flow tube is connected to enable the flow tube to elastically abut the reactor.

11. A sequencing system comprising the carrier of any one of claims 1-10.