CN212199262U - Germ detecting instrument based on laser spectrum technology - Google Patents
Germ detecting instrument based on laser spectrum technology Download PDFInfo
- Publication number
- CN212199262U CN212199262U CN202020307375.3U CN202020307375U CN212199262U CN 212199262 U CN212199262 U CN 212199262U CN 202020307375 U CN202020307375 U CN 202020307375U CN 212199262 U CN212199262 U CN 212199262U
- Authority
- CN
- China
- Prior art keywords
- signal
- laser
- pulse generator
- image intensifier
- ccd
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001228 spectrum Methods 0.000 title claims abstract description 14
- 238000005516 engineering process Methods 0.000 title claims description 7
- 238000010168 coupling process Methods 0.000 claims abstract description 12
- 238000005859 coupling reaction Methods 0.000 claims abstract description 12
- 230000008878 coupling Effects 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 239000000523 sample Substances 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 239000013307 optical fiber Substances 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 9
- 230000003321 amplification Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 17
- 239000000835 fiber Substances 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000003595 spectral effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 10
- 238000002189 fluorescence spectrum Methods 0.000 description 7
- 241000700605 Viruses Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000005284 excitation Effects 0.000 description 3
- 238000001307 laser spectroscopy Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000014670 detection of bacterium Effects 0.000 description 2
- 230000010460 detection of virus Effects 0.000 description 2
- 238000001506 fluorescence spectroscopy Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000010183 spectrum analysis Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 238000012031 short term test Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model provides a germ detecting instrument based on laser spectrum technique, including pulse generator, pulse generator triggers laser instrument transmission ultraviolet laser, is equipped with coupling lens, Y type optic fibre, light filter and beam splitting system along the light path in proper order, and wherein ultraviolet laser gets into the transmitting terminal of Y type optic fibre through the coupling lens coupling, through the fluorescence signal that ultraviolet laser arouses the liquid to be measured and produce is received to the probe of Y type optic fibre, and this fluorescence signal incides the light filter through the receiving terminal of Y type optic fibre, and the light signal after the filtering processing incides beam splitting system, and the light signal after the beam splitting carries out photoelectric signal by the image intensifier and enlargies, the image intensifier is connected with the high-voltage source, by the high-voltage source provides high-voltage power source, the high-voltage source is controlled by pulse generator, the image intensifier is connected with CCD, and spectral signal formation of image is on the CCD surface, the CCD is connected with a signal processing system. The detection instrument has the advantages of small volume, portability, high detection speed and the like.
Description
Technical Field
The utility model relates to a germ detecting instrument technical field especially relates to a germ detecting instrument based on laser spectroscopy technique.
Background
At present, a rapid detection method for viruses and bacteria has become a hot point of research, and the current real-time fluorescence quantitative PCR nucleic acid detection method can be applied to the detection of bacteria and viruses, but has the following disadvantages: the whole operation process is complicated, long in time consumption, needs to be operated by professional personnel, needs to be subjected to centralized inspection, and the detection result is easily influenced by various factors.
The fluorescence spectroscopy has the advantages of high sensitivity, convenience and quickness in detection and the like, and is widely applied to detection of bacteria and viruses, but when toxic microorganisms are detected by adopting an instrument for detecting germs by adopting the fluorescence spectroscopy in the prior art, the toxic microorganisms are small in size and low in microorganism content in a detection sample, so that a fluorescence signal is weak, and a more accurate detection result cannot be achieved.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem that exists among the prior art, the utility model provides a germ detecting instrument based on laser spectrum technique, it has advantages such as small, portable, detection speed is fast, can be used for harmful microorganism's on-the-spot short-term test.
In order to achieve the above object, the utility model provides a germ detecting instrument based on laser spectrum technology, including pulse generator, pulse generator triggers laser instrument transmission ultraviolet laser, is equipped with coupling lens, Y type optic fibre, light filter and beam splitting system along the light path in proper order, and wherein ultraviolet laser gets into the transmitting terminal of Y type optic fibre through the coupling lens coupling, receives the fluorescence signal that ultraviolet laser arouses the liquid to be measured and produce through the probe of Y type optic fibre, and this fluorescence signal incides the light filter through the receiving terminal of Y type optic fibre, and the light signal after the filtering processing incides beam splitting system, and the light signal after the beam splitting is carried out photoelectric signal by the image intensifier and is enlargied, the image intensifier is connected with the high-pressure source, by the high-pressure source provides the high-pressure power supply, the high-pressure source is controlled by pulse generator, the image intensifier is connected with CCD, the spectrum signal is imaged on the surface of the CCD, and the CCD is connected with a signal processing system.
The utility model has the advantages that the germ detecting instrument based on the laser spectrum technology has the advantages of small volume, portability, high detecting speed and the like, and can be used for the on-site rapid detection of harmful microorganisms; ultraviolet laser with proper wavelength is selected to excite different microorganisms to generate characteristic fluorescence spectra, the detection instrument is adopted to detect, and the types of the microorganisms can be identified through spectral analysis.
Drawings
Fig. 1 shows a schematic structural diagram of a pathogen detection apparatus based on laser spectroscopy according to the present invention.
Reference numerals: 1-pulse generator, 2-laser, 3-coupling lens, 4-Y type optical fiber, 5-optical filter, 6-light splitting system, 7-high voltage source, 8-image intensifier, 9-CCD, 10-signal processor and A-liquid to be measured.
Detailed Description
The following description will further describe embodiments of the present invention with reference to the accompanying drawings.
As shown in fig. 1, the pathogen detection apparatus based on laser spectroscopy technology of the present invention comprises a pulse generator 1, wherein the pulse generator 1 triggers a laser 2 to emit ultraviolet laser, a coupling lens 3, a Y-shaped optical fiber 4, an optical filter 5 and a light splitting system 6 are sequentially disposed along a light path, wherein the ultraviolet laser enters a transmitting end of the Y-shaped optical fiber 4 through the coupling lens 3, a probe of the Y-shaped optical fiber 4 detects a liquid a to be detected, specifically, the liquid a to be detected can be in a test tube, on a flat plate or on other carriers, the probe of the Y-shaped optical fiber 4 receives a fluorescence signal generated by exciting the liquid a to be detected by the ultraviolet laser, the fluorescence signal is incident to the optical filter 5 through a receiving end of the Y-shaped optical fiber 4 for light filtering treatment, then incident to the light splitting system 6 for light splitting, and an optical signal after light splitting is amplified by an image intensifier 8, the image intensifier 8 is connected with a high-voltage source 7, the high-voltage source 7 provides high-voltage power, the high-voltage source 7 is controlled by the pulse generator 1, namely the pulse generator 1 triggers the image intensifier 8 to work, the image intensifier 8 is connected with a CCD9, a spectrum signal is imaged on the surface of a CCD9, and the CCD9 is connected with a signal processing system 10, and the spectrum signal is input into the signal processing system 10 to be processed so as to detect spectrum information for identifying germs.
In order to improve fluorescence intensity, increase fluorescence detection sensitivity, the utility model discloses mainly adopt two kinds of measures to improve instrument detection performance, firstly laser source adopts ultraviolet laser, and laser is not only monochromatic good, and intensity is far more than light sources such as ultraviolet LED, ultraviolet lamp moreover, has consequently increased fluorescence intensity. In addition, the low-light-level image intensifier is used as a spectrum detector, and the sensitivity of the detection instrument is greatly improved through synchronous time sequence control of the laser pulse and the image intensifier.
The utility model relates to a germ detecting instrument based on laser spectrum technology, which has the advantages of small volume, portability, high detecting speed and the like, and can be used for the on-site rapid detection of harmful microorganisms; ultraviolet laser with proper wavelength is selected to excite different microorganisms to generate characteristic fluorescence spectra, the detection instrument is adopted to detect, and the types of the microorganisms can be identified through spectral analysis.
At present, the research work of inducing the biological substance to generate the fluorescence spectrum by using laser (especially ultraviolet laser) has achieved related results, and the wavelength range of the fluorescence spectrum of the biological substance is mainly 300-800 nm. Therefore, by selecting a proper ultraviolet laser wavelength, high-efficiency fluorescence excitation of the virus sample can be realized, and currently selectable laser excitation wavelengths comprise: 266nm, 355nm, 405nm, 450nm, etc. The fluorescence spectrum of an organic substance having a single component does not change due to the wavelength of the excitation light; in the case of more complex biological mixtures, such as fungi, bacteria, viral pollen, spores, etc., these are composed of fluorescent molecules such as coenzymes, tryptophan, riboflavin, tyrosine, etc., which differ greatly in their proportions and structures. Therefore, the fluorescence spectrum of the mixed organic substance is obtained by superimposing fluorescence spectra generated by various fluorescent molecules.
Claims (1)
1. A germ detecting instrument based on laser spectrum technology is characterized in that: the device comprises a pulse generator, wherein the pulse generator triggers a laser to emit ultraviolet laser, a coupling lens, a Y-shaped optical fiber, an optical filter and a light splitting system are sequentially arranged along a light path, the ultraviolet laser enters the emitting end of the Y-shaped optical fiber through the coupling lens in a coupling mode, a probe of the Y-shaped optical fiber receives a fluorescent signal generated by exciting liquid to be detected by the ultraviolet laser, the fluorescent signal enters the optical filter through the receiving end of the Y-shaped optical fiber, an optical signal after optical filtering processing enters the light splitting system, the optical signal after optical splitting is subjected to photoelectric signal amplification by an image intensifier, the image intensifier is connected with a high-voltage source, the high-voltage source is provided with a high-voltage power source, the high-voltage source is controlled by the pulse generator, the image intensifier is connected with a CCD, a spectrum signal is imaged on the surface of the CCD, and the CCD.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020307375.3U CN212199262U (en) | 2020-03-13 | 2020-03-13 | Germ detecting instrument based on laser spectrum technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020307375.3U CN212199262U (en) | 2020-03-13 | 2020-03-13 | Germ detecting instrument based on laser spectrum technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212199262U true CN212199262U (en) | 2020-12-22 |
Family
ID=73821501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020307375.3U Active CN212199262U (en) | 2020-03-13 | 2020-03-13 | Germ detecting instrument based on laser spectrum technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212199262U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115389019A (en) * | 2022-09-07 | 2022-11-25 | 西安应用光学研究所 | A weak light spectrum enhancer |
-
2020
- 2020-03-13 CN CN202020307375.3U patent/CN212199262U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115389019A (en) * | 2022-09-07 | 2022-11-25 | 西安应用光学研究所 | A weak light spectrum enhancer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6255118B1 (en) | Method for using an all solid-state fluorometer in industrial water system applications | |
| WO2016124083A1 (en) | Superminiature multi-channel real-time fluorescence spectrometer | |
| EP1582860A1 (en) | Method and apparatus for detecting and imaging the presence of biological materials | |
| KR20010013682A (en) | Solid-state fluorometer and methods of use therefor | |
| EP2889365B1 (en) | Method for examining microorganism and device for same | |
| CN101906384B (en) | Instrument and method for bacterium flow count testing | |
| CN204462019U (en) | A kind of subminiaturization hyperchannel real-time fluorescence spectrum detection device | |
| CN101464409A (en) | Apparatus and method for fast quantitative bacteria detection | |
| CN105628658A (en) | Optical detection system for bioaerosol and detection method | |
| CN2921831Y (en) | High performance capillary electrophoresis instrument | |
| CN107991275A (en) | A kind of optical fiber dissolved oxygen detection method and device based on fluorescent quenching | |
| CN212199262U (en) | Germ detecting instrument based on laser spectrum technology | |
| CN106970058A (en) | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face | |
| US6060318A (en) | Tracing of process additives in industrial ceramics applications | |
| JPWO2002064818A1 (en) | Method and apparatus for immediate identification of fungi | |
| KR200458306Y1 (en) | High sensitivity portable FRET photometer | |
| US7211377B1 (en) | Method for detecting the presence of dormant cryptobiotic microorganisms | |
| CN204514810U (en) | Laser-induced fluorescence detection system | |
| CN206684048U (en) | A kind of minimal feeding instrument in double fluorescent emission faces | |
| CN102323244A (en) | Method for rapidly detecting portable food packaging fluorescent material and device | |
| CN1226611C (en) | Multiphoton excitation capillary electrophoresis fluorescence detector based on continuous light | |
| KR101806763B1 (en) | Proactive portable algae detecting apparatus | |
| CN204495717U (en) | The detection of the fluorescence emission wavelengths of biological vestige and visualization means thereof | |
| CN2646699Y (en) | Equipment for deducting shifting and pulsing of light source for atomic fluorescence spectrometer | |
| Zhang et al. | Development and calibration of a single UV LED based bioaerosol monitor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 264200 first floor, annex B1, building 54, Lushan Road, Zhangcun Town, Huancui District, Weihai City, Shandong Province Patentee after: Hasunda (Weihai) Photoelectric Technology Co.,Ltd. Address before: 706, block a, Haiyu City, No. 3-2, Shichang Avenue, Huancui District, Weihai City, Shandong Province Patentee before: Weihai Chengyue Photoelectric Technology Co.,Ltd. |