CN211987210U - High-flux cross-contamination-prevention solid phase extraction device - Google Patents

Abstract

The utility model discloses a high-throughput anti-cross-contamination solid-phase extraction device. The device comprises a cover plate and a vacuum container. A test tube rack is arranged in the vacuum container, and a suction nozzle is arranged on the side wall; A pressure gauge and a pressure relief valve are arranged on the wall, a plurality of through holes are arranged on the panel of the cover plate, and a detachable universal joint is arranged in each through hole, and the center of the universal joint is provided with a liquid guiding needle penetration hole, The needle end of the catheter needle passes through the bottom of the universal joint, and the joint end is connected with the flow control valve, and the flow control valve is connected with the SPE small column; the catheter needle is a standard industrial dispensing bayonet needle. The device is provided with multiple sample extraction paths, and is equipped with independently disassembled universal joints. Each universal joint is equipped with a standard industrial needle, which reduces the procurement cost of special liquid-guiding needles for solid-phase extraction devices on the market; universal joints and liquid-guiding needles The detachable design of the needle truly achieves the purpose of preventing cross-contamination in solid phase extraction.

Description

A high-throughput anti-cross-contamination solid-phase extraction device

technical field

The utility model belongs to the technical field of sample pretreatment devices, in particular to a high-throughput anti-cross-contamination solid-phase extraction device.

Background technique

Solid phase extraction (SPE) is a sample pretreatment technology developed in recent years. It is developed from the combination of liquid-solid extraction and column liquid chromatography. It is mainly used for the separation, purification and concentration of samples. It is different from traditional liquid-liquid extraction. Compared with other methods, it can improve the recovery rate of analytes, separate analytes from interfering components more effectively, and reduce the sample pretreatment process. The operation is simple, time-saving and labor-saving. Features. Solid-phase extraction can be divided into four types according to its similar compatibility mechanism: reversed-phase SPE, normal-phase SPE, ion-exchange SPE, and adsorption SPE.

Solid phase extraction is mostly used to process liquid samples, extract, concentrate and purify semi-volatile and non-volatile compounds in them, and can also be used to process solid samples. First, the solid samples must be treated with a solvent to dissolve the components to be extracted in In the solvent, the extraction is carried out in the form of a solvent. At present, solid phase extraction technology is widely used in medicine, food, environment, commodity inspection, chemical industry and other fields. It is an important sample pretreatment method in food safety, environmental monitoring, drug analysis and other analysis and testing.

The solid phase extraction device used in the early days is a simple SPE column with packing. The SPE column consists of a column tube, a packing and a push rod. The flow of the eluent in the column tube is realized by manually pushing the push rod. Although this method is simple to operate , but the extraction efficiency is low and the time consumption is long, which increases the operation intensity of the experimenter.

With the development of solid phase extraction technology, SPE devices have gradually come out. They are composed of SPE cartridges and accessories. SPE cartridges are composed of three parts, column tube, sintering pad and packing. SPE accessories generally include vacuum system, vacuum pump, Drying device, inert gas source, large-capacity sampler and buffer bottle, the device can process multiple different samples at the same time, with high extraction efficiency, which reduces the labor intensity of the experimenter. At the same time, each channel has an independent flow control valve, which can Adjust the flow rate for each individual channel. However, this device has certain defects. The catheter connected to the SPE column on the cover is generally fixed on the bottom surface of the cover. When changing different extraction samples, the catheter part needs to be cleaned at the same time. It is cumbersome, increases labor intensity, and will inevitably cause the risk of cross-contamination after repeated use. Although the prior art has developed an anti-cross-contamination type solid phase extraction device, the device is used to prevent the occurrence of cross-contamination by replacing the catheter on the cover plate, however, the replacement catheter part is used as a supporting auxiliary for the solid phase extraction device It must be purchased together with the device, and the special catheter purchased as a package is expensive, which greatly increases the extraction cost when processing a large number of samples. On the other hand, although the catheter can be used once, during the process of replacing the catheter, the residual sample at the end of the catheter may contaminate the catheter penetration hole. When a new catheter is inserted, Contaminated catheter penetration holes may lead to secondary contamination of new catheters, which cannot achieve true cross-contamination prevention.

Utility model content

In order to solve the above problems existing in the prior art, the purpose of the present invention is to provide a high-throughput anti-cross-contamination solid-phase extraction device, which can not only meet the requirements of high-throughput anti-cross-contamination, but also greatly reduces the extraction cost.

In order to achieve the above object, the utility model provides the following technical solutions:

A high-throughput anti-cross-contamination solid-phase extraction device, the device comprises a cover plate and a vacuum container, a test tube rack is arranged in the vacuum container, and a suction nozzle is arranged on the side wall of the vacuum container; The side wall of the cover plate is provided with a pressure gauge and a pressure relief valve, the panel of the cover plate is provided with a plurality of through holes, each through hole is provided with a detachable universal joint, and the center of the universal joint is provided with a detachable universal joint. There is an insertion hole for the catheterization needle, the needle end of the catheterization needle penetrates the bottom of the universal joint, and the joint end is connected with a flow control valve, and the flow control valve is connected with the SPE small column.

Further, the catheter needle is a standard industrial dispensing bayonet needle.

Further, the bottom surface of the contact surface of the cover plate and the vacuum container is provided with a sealing gasket, and each corner of the bottom surface of the cover plate is provided with a cover plate support foot.

Further, the through holes on the cover plate are sequentially arranged from top to bottom as a first channel and a second channel, the inner diameter of the second channel is smaller than the inner diameter of the first channel, the first channel and the second channel are The connecting surface forms a first stop; the outer diameter of the first section of the universal joint matches the inner diameter of the first hole, the outer diameter of the second section of the universal joint matches the inner diameter of the second hole, and the universal joint matches the inner diameter of the second hole. The connector is clamped in the through hole and limited by the first limit stage.

Further, the head of the first section of the universal joint is exposed on the top surface of the cover plate.

Further, the outer periphery of the top of the penetration hole of the liquid guiding needle is provided with a ring of grooves to form a second limiting platform, and the needle end of the liquid guiding needle is limited on the universal joint by the second limiting platform.

Further, the test tube rack is composed of a support base plate, a plurality of test tube placement plates and a plurality of height adjustment rods, the plurality of height adjustment rods are fixed on the support base plate, and a plurality of test tube placement plates are parallelly penetrated on the height adjustment rods. .

Further, a tray is placed at the bottom of the vacuum container, the tray is provided with drainage holes, and two opposite side walls of the tray are provided with a pair of hand-held slots.

Beneficial effects: The utility model discloses a high-throughput anti-cross-contamination solid-phase extraction device, the device is provided with a plurality of sample extraction passages, and is provided with an independently disassembled universal joint, which can be matched with different materials, different Standard industrial needles of different lengths and thicknesses do not require special design and production, which greatly reduces the procurement cost of special liquid-guiding needles for solid-phase extraction devices on the market. The disassembly design makes it also possible to use it as a disposable part, which ensures that each sample extraction operation can use a new universal joint and a liquid guide needle, which truly realizes the purpose of preventing cross-contamination of solid phase extraction.

Description of drawings

FIG. 1 is a schematic structural diagram of the solid phase extraction device according to the present invention.

FIG. 2 is a schematic structural diagram of the cover plate according to the present invention.

Fig. 3 is a partial cross-sectional view of the cover plate of the present invention after the universal joint is assembled.

FIG. 4 is a schematic structural diagram of the test tube rack according to the present invention.

FIG. 5 is a schematic structural diagram of the tray according to the present invention.

In the figure: 1. Cover plate; 2. Vacuum container; 3. Test tube rack; 31. Support bottom plate; 32. Test tube placement plate; 33. Height adjustment rod; 4. Air extraction nozzle; 5. Pressure gauge; 6. Pressure relief Valve; 7, through hole; 71, first orifice; 72, second orifice; 8, universal joint; 81, catheterization needle penetration hole; 82, catheterization needle; ; 85, the second limit stage; 9, the tray; 91, the drainage hole; 92, the hand slot; 10, the flow control valve; 11, the cover plate support foot.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments, but the embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention .

A high-throughput anti-cross-contamination solid-phase extraction device, as shown in Figure 1, includes a cover plate 1 and a vacuum container 2, and the material of the vacuum container 2 can be a thickened and pressure-resistant glass cylinder; the vacuum container There is a test tube rack 3 inside, and a suction nozzle 4 is arranged on the side wall of the vacuum container 2. The suction nozzle 4 uses a conduit to connect a buffer bottle, and the buffer bottle is connected to the vacuum pump through a conduit. The conduit can be selected from standard plastic steel. Conduit or all-plastic conduit, the buffer bottle can be equipped with a standard GL45 safety buffer bottle cap; a pressure gauge 5 and a pressure relief valve 6 are arranged on the side wall of the cover plate 1, and the panel of the cover plate 1 is provided with multiple There are 7 through holes, the number of through holes determines the number of samples, and a three-way valve can also be equipped with each through hole to increase the number of samples in each through hole.

Each through hole 7 is provided with a detachable universal joint 8, the center of the universal joint 8 is provided with a liquid guide needle penetration hole 81, and the needle end of the liquid guide needle 82 passes through the bottom of the universal joint 8, and its The joint end is connected with the flow control valve 10, and the flow control valve 10 is connected with the SPE small column; wherein the catheter needle 82 is a standard industrial dispensing bayonet needle, the length of the needle tube is between 38mm and 100mm, and the specifications are: 22#-14# can be used. The needle can be purchased in the market. It can be purchased from a wide range of sources and is cheap. It can be made of all metal, plastic steel or all PP. can be), no special design and production are required, which greatly reduces the procurement cost of the special liquid-guiding needles for solid-phase extraction devices on the market; at the same time, the general-purpose joints can also be mass-produced, and the detachable design of the general-purpose joints makes the It can also be used as a disposable part, which ensures that each sample extraction operation can use a new universal joint and a liquid guide needle, which truly realizes the purpose of preventing cross-contamination of solid phase extraction.

The bottom surface of the contact surface of the cover plate 1 and the vacuum container 2 is provided with a sealing gasket, and the arrangement of the sealing gasket makes the device have good sealing performance; When the user removes the cover from the device, it can be conveniently placed on the workbench.

As a matching design method of universal joints and through holes, as shown in FIGS. 2 and 3 , the through holes 7 on the cover plate 1 are sequentially arranged from top to bottom as a first hole 71 and a second hole 72 . The inner diameter of the second hole 72 is smaller than the inner diameter of the first hole 71 , and the connecting surface of the first hole 71 and the second hole 72 forms a first stop; the outer diameter of the first section 83 of the universal joint 8 is the same as that of the first The inner diameter of the hole 71 matches, the outer diameter of the second section 84 of the universal joint 8 matches the inner diameter of the second hole 72 , and the universal joint is clamped in the through hole and limited by the first limit stage. The universal joint is made of rubber material, and is assembled in the through hole by means of interference fit, thereby ensuring good sealing between the universal joint and the through hole.

In the design method of matching the universal joint and the through hole, the structures of the two can also be designed into other alternative forms, as long as the universal joint is detachably fixed in the through hole, and the universal joint can still be used during the vacuuming process. It is sufficient to maintain good fixation and good sealing with the through hole. Meanwhile, as a preferred embodiment, the universal connector can also be designed with reference to the structure of the existing Luer connector, and then the through hole can be designed into a matching structure.

Preferably, the head of the first section 83 of the universal joint 8 is exposed on the top surface of the cover plate 1 , and the user can easily replace and assemble the universal joint only by holding the head.

Preferably, as shown in FIG. 3 , the outer periphery of the top of the penetration hole 81 of the liquid guiding needle is provided with a circle of grooves to form a second limit platform 85 , and the needle end of the liquid guiding needle is limited by the second limit table On the universal joint, so that the catheter needle can maintain a good fixation during the extraction process.

As shown in FIG. 4 , the test tube rack 3 is composed of a bracket bottom plate 31, a plurality of test tube placement plates 32 and a plurality of height adjustment rods 33. The plurality of height adjustment rods 33 are fixed on the bracket bottom plate 31, and a plurality of test tubes are placed The plate 32 is arranged in parallel on the height adjustment rod 33; the test tube placement plate can accommodate test tubes or centrifuge tubes of different volumes.

As shown in FIG. 5 , a tray 9 is placed on the bottom of the vacuum container 2 , the tray 9 is provided with a drainage hole 91 , and the two opposite side walls of the tray are provided with a pair of hand-held slots 92 . After the vacuum container is cleaned, the water on the outer wall can be drained through the drain hole 91, which avoids the problem of sliding of the vacuum container in the presence of water; the hand-held slotted design facilitates the movement of the device.

The cover plate, the support feet of the cover plate, the test tube rack and the tray are all made of plastic materials of high anti-corrosion level, so as to improve the use performance of the device.

The device is used as follows:

1. Put the test tube rack into the vacuum container, and put the test tube of the corresponding specification on the test tube rack;

2. Connect the catheter to the suction nozzle of the vacuum container, and then connect the buffer bottle and the vacuum pump in turn;

3. Put the cover plate on the vacuum container, and each through hole corresponds to the test tube one by one;

4. Assemble the universal connector in the through hole, then insert the catheter needle, and assemble the flow control valve and SPE small column;

5. Close the flow control valve and tighten the pressure relief valve;

6. Add the sample to be extracted into the SPE cartridge;

7. Open the flow control valve and vacuum pump, the liquid flows into the test tube through the SPE cartridge under vacuum, and the flow rate of the liquid can be controlled by adjusting the flow control valve during the extraction process;

8. When the extraction is completed, turn off the vacuum pump and unscrew the pressure relief valve to release the pressure in the vacuum container;

9. Collect the extracted samples, remove the universal joint and catheter needle, wash or discard them, and then replace with a new universal joint and catheter needle, and proceed to the next round of extraction.

Claims (8)

1. A high-flux cross-contamination prevention type solid phase extraction device is characterized by comprising a cover plate and a vacuum container, wherein a test tube rack is arranged in the vacuum container, and an air suction nozzle is arranged on the side wall of the vacuum container; the lateral wall of the cover plate is provided with a pressure gauge and a pressure relief valve, the panel of the cover plate is provided with a plurality of through holes, a detachable universal joint is arranged in each through hole, the center of the universal joint is provided with a liquid guide needle penetrating hole, the needle end of the liquid guide needle penetrates out of the bottom of the universal joint, the joint end of the liquid guide needle is connected with a flow control valve, and the flow control valve is connected with the SPE small column.

2. The high-throughput cross-contamination prevention type solid-phase extraction device of claim 1, wherein the liquid guide needle is a standard industrial dispensing bayonet needle.

3. The high-throughput cross-contamination prevention solid phase extraction device according to claim 1, wherein a sealing gasket is provided on the bottom surface of the contact surface of the cover plate and the vacuum vessel, and a cover plate supporting leg is provided on each corner of the bottom surface of the cover plate.

4. The high-throughput cross-contamination prevention solid phase extraction device according to claim 1, wherein the through hole of the cover plate is sequentially provided with a first hole channel and a second hole channel from top to bottom, the inner diameter of the second hole channel is smaller than that of the first hole channel, and the joint surface of the first hole channel and the second hole channel forms a first limit platform; the first section external diameter of universal joint and the internal diameter phase-match of first pore, the second section external diameter of universal joint and the internal diameter phase-match of second pore, the universal joint carries out spacingly in the through-hole and through first spacing platform.

5. The high throughput cross-contamination prevention solid phase extraction device of claim 4, wherein the first section of the universal joint is exposed at the top surface of the cover plate.

6. The high-throughput cross-contamination prevention type solid phase extraction device according to claim 1, wherein a circle of groove is formed on the periphery of the top of the liquid guide needle penetrating hole to form a second limiting table, and the needle end of the liquid guide needle is limited on the universal joint through the second limiting table.

7. The high-throughput cross-contamination prevention solid-phase extraction device according to claim 1, wherein the test tube rack comprises a rack bottom plate, a plurality of test tube placing plates and a plurality of height adjusting rods, the height adjusting rods are fixed on the rack bottom plate, and the plurality of test tube placing plates are parallelly arranged on the height adjusting rods in a penetrating manner.

8. The high-throughput cross-contamination prevention solid phase extraction device of claim 1, wherein a tray is placed at the bottom of the vacuum vessel, the tray is provided with a drain hole, and a pair of hand-held slots are formed on two opposite side walls of the tray.

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