CN211826074U - Immunofluorescence automatic constant temperature mechanism - Google Patents

Abstract

The utility model discloses an immunofluorescence automatic constant temperature mechanism, comprising: an incubator body, a temperature control module and a fluorescence detection module, wherein the incubator body comprises a base and an upper shell, and a test chamber is embedded in the top of the base , the bottom outer side of the base is embedded with a cavity for installing a heating device and a lead slot, the front of the base is provided with a first bayonet slot, and the upper shell is movably arranged on the upper part of the base, The temperature control module includes a temperature sensor, a fuse, a circuit board and a management circuit arranged on the circuit board, and the fluorescence detection module is arranged inside the incubator body. The utility model uses a constant temperature heating film to form a reaction environment of 37 degrees, promotes better reaction of the reactants, and increases the sensitivity of the test results. The use of temperature regulation algorithms and error proofing design makes the system more robust. The heat transfer through the aluminum plate makes the heating more stable and the thermal balance in the space is better.

Description

A kind of immunofluorescence automatic constant temperature mechanism

technical field

The utility model relates to the technical field of detection, in particular to an immunofluorescence automatic constant temperature mechanism.

Background technique

Immunofluorescence test is a detection method based on the specific combination of antigen and antibody. The designed immune response has the best binding environment at the human body temperature of 37 degrees Celsius.

At present, in order to save space, reduce power consumption, and adapt to mobile applications, immunofluorescence instruments generally design heating modules. By continuously monitoring the temperature change after heating, if the temperature change is lower than the threshold, the heating film is considered to be invalid and the software reports an error. If the temperature changes too much and continues to rise after stopping, the physical thermal melt control will interrupt the circuit to achieve protection.

Utility model content

The technical problem to be solved by the utility model is to provide a simple structure and an immunofluorescence automatic constant temperature mechanism.

In order to solve the above-mentioned technical problems, the technical scheme of the present utility model is:

An immunofluorescence automatic constant temperature mechanism, comprising:

The thermostatic box body includes a base and an upper shell, the upper part of the base is embedded with a test chamber, and the bottom outer side of the base is embedded with a cavity for installing a heating device and a lead groove, so The front of the base is provided with a first bayonet slot, the upper two sides of the upper shell are symmetrically arranged, and a plurality of circular through holes are symmetrically arranged on the two sides, and the front of the upper shell corresponds to the The first bayonet slot is provided with a second bayonet slot, and the upper shell is movably arranged on the upper part of the base;

A temperature control module, the temperature control module includes a temperature sensor, a fuse, a circuit board and a management circuit arranged on the circuit board, the temperature sensor and the fuse are respectively electrically connected to the circuit board, and the fuse is electrically connected to the circuit board. the heating device is electrically connected, and the fuse is arranged in the cavity;

A fluorescence detection module is provided inside the incubator body.

Preferably, the fluorescence detection module includes a light source, a detection and detection light path, and an area array image sensor.

Preferably, there are multiple temperature sensors, and the multiple temperature sensors are respectively disposed inside and at the bottom of the incubator body.

Preferably, a bracket is arranged inside the base, the bracket is arranged corresponding to the bayonet slot, and a plurality of screw columns and fixing columns are arranged inside the base.

Preferably, an L-shaped installation step is embedded on the top surface of the base along the inner periphery, and a square groove is formed through the L-shaped installation step on the upper part of the side surface of the base.

Preferably, a plurality of the screw columns are symmetrically arranged.

Preferably, the heating device is a heating film, the heating film is a silicone electric heating film, a PT electric heating film, and a PET electric heating film, the heating film is provided with a silicone insulating layer outside, and the heating device is fixedly arranged on The bottom of the base is provided with thermal conductive paste between the heating film and the base.

Preferably, a plurality of connecting posts are arranged inside the upper shell, and the plurality of connecting posts correspond to a plurality of the screw posts, and the upper shell and the base are screwed together.

The beneficial effects of the present utility model are:

The utility model uses a constant temperature heating film to form a reaction environment of 37 degrees, promotes better reaction of the reactants, and increases the sensitivity of the test results. The use of temperature regulation algorithms and error proofing design makes the system more robust. The heat transfer through the aluminum plate makes the heating more stable and the thermal balance of the space is better.

Description of drawings

1 is a schematic structural diagram of a base in an embodiment of an immunofluorescence automatic constant temperature mechanism of the present invention;

FIG. 2 is a structural exploded view of an embodiment of an immunofluorescence automatic constant temperature mechanism of the present invention.

In the figure, 1-base, 2-upper shell, 3-heating device, 4-lead groove, 5-support, 6-transparent window.

Detailed ways

The specific embodiments of the present utility model will be further described below with reference to the accompanying drawings. It should be noted here that the description of these embodiments is used to help the understanding of the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as there is no conflict with each other.

As shown in Figure 1 and Figure 2, an immunofluorescence automatic constant temperature mechanism includes:

The thermostatic box body includes a base 1 and an upper shell 2. The upper part of the base 1 is embedded with a test chamber, and the bottom outer side of the base 1 is embedded with a cavity for installing the heating device 3 and a lead groove 4. The front of the seat 1 is provided with a first bayonet slot, the upper two sides of the upper shell 2 are symmetrically arranged, and the two sides are symmetrically provided with a plurality of circular through holes, and the front of the upper shell 2 is provided with a corresponding first bayonet slot. The second bayonet slot, the upper shell 2 is movably arranged on the upper part of the base 1;

The temperature control module includes a temperature sensor, a fuse, a circuit board and a management circuit arranged on the circuit board. The temperature sensor and the fuse are respectively electrically connected to the circuit board, and the fuse is electrically connected to the heating device. set in the cavity;

The fluorescence detection module is arranged inside the incubator body.

In a preferred embodiment of the present invention, the fluorescence detection module includes a light source, a detection and detection optical path, and an area array image sensor.

Specifically, in this embodiment, the structure of the fluorescence detection module is as follows: a point excitation light source, a detection optical path, and a non-image photoelectric sensor are used and placed on the top surface, bottom surface or side surface of all PCR test tubes. The image photoelectric sensor includes a photodiode, a photocell, a photocell, and a photomultiplier tube. The point excitation light source irradiates and excites a PCR sample solution at a time, and the non-image photoelectric sensor realizes the fluorescence signal of the sample solution in a PCR test tube. detection; or use a surface excitation light source, a detection optical path and an area array image sensor and place them on the top, bottom or side surfaces of all PCR test tubes, the surface excitation light source illuminates all at one time and simultaneously excites all PCR sample solutions, The area array image sensor simultaneously detects the fluorescent signal of the sample solution in all PCR test tubes.

In a preferred embodiment of the present invention, there are multiple temperature sensors, and the multiple temperature sensors are respectively arranged inside and at the bottom of the thermostatic box body.

Specifically, in this embodiment, a fault-tolerant design is added, including thermal fusing, control failure judgment, and the like. The implementation method is to continuously monitor the temperature change after heating. If the temperature change is lower than the threshold value, it is considered that the added film fails, and the software reports an error. If the temperature change is too large and continues to rise after stopping, it is considered that the control is achieved, and the physical hot melt control will interrupt the circuit to achieve protection.

Specifically, in this embodiment, the surface of the incubator body is provided with two symmetrical operation holes with circular hole-like structures, and the side of the incubator body is provided with a transparent window.

In a preferred embodiment of the present invention, a bracket is arranged inside the base 1, the bracket is arranged corresponding to the bayonet slot, and a plurality of screw columns and fixing columns are arranged inside the base.

Specifically, in this embodiment, there are two brackets, and the two brackets are arranged in parallel.

In a preferred embodiment of the present invention, the top surface of the base 1 is embedded with an L-shaped installation step along the inner periphery, and the upper side of the base 1 is provided with a square groove through the L-shaped installation step.

Specifically, in this embodiment, the base 1 is made of aluminum material.

In a preferred embodiment of the present invention, a plurality of screw posts are symmetrically arranged.

In a preferred embodiment of the present invention, the heating device 3 is a heating film, and the heating film is a silicone electric heating film, a PT electric heating film, and a PET electric heating film. The heating film is provided with a silicone insulating layer outside, and the heating device 3 is fixedly arranged on the At the bottom of the base 1 , a thermal conductive paste is provided between the heating film and the base 1 .

Specifically, in this embodiment, the heating film is used as the heat source of the heating module, and the heat is transferred to the slot of the detection card through the aluminum base of the machined part to form a constant temperature environment in the space of the detection optical module. Temperature reduction is achieved through natural heat dissipation. According to the detection of the internal and external ambient temperature of the optical module, the temperature adjustment algorithm is used to achieve constant temperature control.

When the temperature of the detection chamber is too low, the heating film is turned on, and the heat is conducted into the detection chamber when the heating film is working. When the temperature is close to 37 degrees Celsius, stop heating and let the temperature slowly rise close to 37 degrees Celsius. The stop temperature is determined according to the temperature of the external environment. When the external environment temperature is low, the corresponding stop temperature is closer to 37 degrees Celsius; when the outside temperature is high, the stop temperature is farther from 37 degrees Celsius.

In a preferred embodiment of the present invention, the interior of the upper shell 2 is provided with a plurality of connecting columns, the plurality of connecting columns correspond to a plurality of screw columns, and the upper shell 2 and the base 1 are connected by screws.

Specifically, in this embodiment, a fault-tolerant design is added, including thermal fusing, control failure judgment, and the like. By continuously monitoring the temperature change after heating, if the temperature change is lower than the threshold value, it is considered that the added film fails, and the software reports an error. If the temperature change is too large and continues to rise after stopping, it is considered that the control is achieved, and the physical hot melt control will interrupt the circuit to achieve protection.

The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, without departing from the principle and spirit of the present invention, various changes, modifications, substitutions and alterations can be made to these embodiments, which still fall within the protection scope of the present invention.

Claims (8)

1. an immunofluorescence automatic constant temperature mechanism, is characterized in that, comprises: The thermostatic box body includes a base and an upper shell, the upper part of the base is embedded with a test chamber, and the bottom outer side of the base is embedded with a cavity for installing a heating device and a lead groove, so The front of the base is provided with a first bayonet slot, the upper two sides of the upper shell are symmetrically arranged, and a plurality of circular through holes are symmetrically arranged on the two sides, and the front of the upper shell corresponds to the The first bayonet slot is provided with a second bayonet slot, and the upper shell is movably arranged on the upper part of the base; A temperature control module, the temperature control module includes a temperature sensor, a fuse, a circuit board and a management circuit arranged on the circuit board, the temperature sensor and the fuse are respectively electrically connected to the circuit board, and the fuse is electrically connected to the circuit board. the heating device is electrically connected, and the fuse is arranged in the cavity; A fluorescence detection module is provided inside the incubator body. 2 . The immunofluorescence automatic constant temperature mechanism according to claim 1 , wherein the fluorescence detection module comprises a light source, a detection and detection light path, and an area array image sensor. 3 . 3 . The immunofluorescence automatic constant temperature mechanism according to claim 1 , wherein there are multiple temperature sensors, and the multiple temperature sensors are respectively arranged inside and at the bottom of the incubator body. 4 . 4 . The immunofluorescence automatic constant temperature mechanism according to claim 1 , wherein a bracket is arranged inside the base, the bracket is arranged corresponding to the bayonet slot, and the inside of the base is arranged with a bracket. 5 . Multiple screw posts and fixed posts. 5 . The immunofluorescence automatic constant temperature mechanism according to claim 1 , wherein the top surface of the base is embedded with an L-shaped installation step along the inner periphery, and the upper side of the base penetrates the L-shaped installation step. 6 . The type mounting steps have square grooves. 6 . The immunofluorescence automatic constant temperature mechanism according to claim 4 , wherein a plurality of the screw rods are symmetrically arranged. 7 . 7. The immunofluorescence automatic constant temperature mechanism according to claim 1, wherein the heating device is a heating film, and the heating film is a silicone electric heating film, a PT electric heating film, and a PET electric heating film, and the A silica gel insulating layer is provided on the outside of the heating film, the heating device is fixedly arranged on the bottom of the base, and a thermal conductive paste is provided between the heating film and the base. 8 . The immunofluorescence automatic constant temperature mechanism according to claim 4 , wherein the upper shell is provided with a plurality of connecting columns, and the plurality of connecting columns correspond to a plurality of the screw columns, and the upper shell , Base screw connection.

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