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CN211426204U - Blood cell analyzer - Google Patents

Blood cell analyzer Download PDF

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CN211426204U
CN211426204U CN201921382950.XU CN201921382950U CN211426204U CN 211426204 U CN211426204 U CN 211426204U CN 201921382950 U CN201921382950 U CN 201921382950U CN 211426204 U CN211426204 U CN 211426204U
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plate
blood cell
lens
blood
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张兆麟
张金泉
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Suzhou kangjianyun Medical Technology Co.,Ltd.
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Suzhou Zhongjia Kangmei Technology Co ltd
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    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements

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Abstract

The application discloses a blood cell analyzer. The blood cell analyzer comprises a sample processing plate, an amplifying counting plate and a blood cell detector; the sample processing plate is used for containing a blood cell sample; the magnifying counting plate comprises a lens; the blood cell detector includes an image sensor; the sample processing plate is detachably mounted on the amplification counting plate, and the amplification counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample amplified by the lens is transmitted to the image sensor. The application provides a hematology analyzer is disposable magnifying glass, accords with clinical examination standard, has guaranteed the reliability of result to this analyzer is with low costs, can use in daily environment.

Description

一种血细胞分析仪A blood cell analyzer

技术领域technical field

本申请涉及一种血细胞分析仪,属于医学仪器技术领域。The application relates to a blood cell analyzer, which belongs to the technical field of medical instruments.

背景技术Background technique

在现代临床检验医学领域,血常规检验是疾病诊断和健康体检最常用、最重要和最基础的检验项目之一。通过该项目的检查可以了解人体血液内各种细胞的大小、数量、形态、比例等数据,以供临床医生诊断治疗疾病,提供体检人群健康指标。In the field of modern clinical laboratory medicine, routine blood test is one of the most commonly used, important and basic test items for disease diagnosis and physical examination. Through the inspection of this project, the size, quantity, shape, proportion and other data of various cells in human blood can be understood, which can be used by clinicians to diagnose and treat diseases, and provide health indicators for people undergoing physical examination.

目前血液常规检查主要有人工检查和仪器检查两种方式。At present, there are two main methods of blood routine examination: manual examination and instrument examination.

人工检查方式:定量吸取血液样本,经过稀释液或溶血剂稀释后,注入改良Neubauer氏血细胞计数板中,在显微镜下按不同要求计数出各种不同血细胞数量,然后通过计算得到各种细胞在一定容积中的数量。同时将同一份血液样本在载玻片上推成血膜,再经过固定染色,在生物显微镜下根据不同形态分类白细胞。该方法优点是通过人的肉眼进行计数和分类,结果比较可靠准确。缺点是速度慢、效率低、操作繁琐,并且现有的生物显微镜需要重复使用,需要专业人员反复调节物镜、视野、焦距等,不同人员使用会影响分析结果的准确性。但此方法仍然保留用于仪器法结果异常时复检,或对于某些特殊病例特殊要求的状况下和没有条件购买仪器的地方使用。Manual inspection method: Quantitatively draw blood samples, after dilution with diluent or hemolytic agent, inject them into the modified Neubauer's blood cell counting plate, count the number of different blood cells under the microscope according to different requirements, and then calculate the various cells in a certain amount. quantity in the volume. At the same time, the same blood sample was pushed into a blood film on a glass slide, and then fixed and stained, and leukocytes were classified according to different morphologies under a biological microscope. The advantage of this method is that it is counted and classified by human eyes, and the results are more reliable and accurate. The disadvantage is that the speed is slow, the efficiency is low, and the operation is cumbersome, and the existing biological microscope needs to be reused, requiring professionals to repeatedly adjust the objective lens, field of view, focal length, etc. The use of different personnel will affect the accuracy of the analysis results. However, this method is still reserved for re-examination when the results of the instrument method are abnormal, or for special cases and places where there are no conditions to purchase instruments.

仪器检查方式:血细胞分析仪根据仪器构成的复杂程度大致可以分为半自动、全自动、白细胞二分类、白细胞三分类、白细胞五分类,目前最常用的是最后两种仪器。首先仪器会自动或半自动吸取抗凝的血液样本,然后自动分配样本到不同容器进行稀释、溶血、染色处理,分别通过红细胞和血小板通道,利用库尔特原理测定出红细胞和血小板的电阻脉冲数和脉冲大小,计数出红细胞和血小板数量。通过白细胞通道,利用库尔特原理测定出白细胞电阻脉冲数和脉冲大小的数量、体积计数出白细胞的数量。同时使用激光、可见光、细胞染色等技术对白细胞进行分类计数。其优点是操作简单、测定速度快、自动化程度高、计数细胞的数量大大超过手工法,因此计数结果准确率高。缺点是仪器精密价格贵、运行环境要求高、日常维护频繁、在细胞形态识别准确性方面比手工法低。Instrument inspection method: blood cell analyzers can be roughly divided into semi-automatic, full-automatic, second-classification, third-classification, and fifth-classification of white blood cells according to the complexity of the instrument. First, the instrument will automatically or semi-automatically draw anticoagulated blood samples, and then automatically distribute the samples to different containers for dilution, hemolysis, and staining, and then pass through the erythrocyte and platelet channels respectively. Pulse size, count the number of red blood cells and platelets. Through the leukocyte channel, the number of leukocyte resistance pulses, the number of pulses, and the volume of the leukocytes were counted by Coulter's principle. At the same time, using laser, visible light, cell staining and other technologies to classify and count white blood cells. The advantages are simple operation, fast measurement speed, high degree of automation, and the number of counted cells greatly exceeds the manual method, so the accuracy of counting results is high. The disadvantage is that the precision of the instrument is expensive, the operating environment requirements are high, the daily maintenance is frequent, and the accuracy of cell morphology recognition is lower than that of the manual method.

实用新型内容Utility model content

根据本申请,提供了一种血细胞分析仪,该血细胞分析仪中的放大计数板有两种结构,第一种放大计数板的双凸球面透镜安装在计数放大板的锥形通孔里,第二种放大计数板的透镜是固定在放大计数板中的主体板外底面的一体式平凸透镜,它们是抛弃式一次性使用的显微放大镜,设定了视野、焦距、放大倍数等参数,符合临床检验标准、保证了结果的可靠性,且可以非专业人员使用进行快速高效的检测分析,并且该分析仪成本低,可以在日常环境中使用。According to the present application, a blood cell analyzer is provided, and the magnifying and counting board in the blood cell analyzer has two structures. The lenses of the two types of magnifying counters are one-piece plano-convex lenses fixed on the outer bottom surface of the main body plate in the magnifying counter. They are disposable disposable microscope magnifiers. The clinical test standard ensures the reliability of the results, and can be used by non-professionals for fast and efficient detection and analysis, and the analyzer is low-cost and can be used in a daily environment.

一种血细胞分析仪,所述血细胞分析仪包括样本处理板、放大计数板和血细胞检测器;A blood cell analyzer, the blood cell analyzer includes a sample processing board, a magnifying counting board and a blood cell detector;

所述样本处理板用于盛放血细胞样本;The sample processing plate is used for holding blood cell samples;

所述放大计数板包括透镜;the magnifying counter board includes a lens;

所述血细胞检测器包括图像传感器;the blood cell detector includes an image sensor;

所述样本处理板可拆卸地安装在所述放大计数板上,所述放大计数板可拆卸地安装在所述血细胞检测器中,以使经过所述透镜放大的所述血细胞样本的光学图像传输给所述图像传感器。The sample processing plate is detachably mounted on the magnifying counter plate, and the magnifying counter plate is detachably mounted in the blood cell detector to transmit an optical image of the blood cell sample magnified through the lens to the image sensor.

可选地,所述样本处理板包括载玻片、支撑件以及盖玻片;Optionally, the sample processing plate includes a glass slide, a support and a cover glass;

在所述载玻片上设有下凹的样本池;A concave sample pool is provided on the glass slide;

所述盖玻片和支撑件均位于所述样品池内,所述盖玻片与所述样品池的底面之间通过所述支撑件形成用于盛放所述血细胞样本的间隙。Both the cover glass and the support are located in the sample pool, and a gap for holding the blood cell sample is formed between the cover glass and the bottom surface of the sample pool through the support.

可选地,所述间隙沿竖直方向的高度为0.05~0.12mm。Optionally, the height of the gap along the vertical direction is 0.05-0.12 mm.

可选地,所述放大计数板还包括主体板;Optionally, the amplifying and counting board further comprises a main body board;

在所述主体板上设有用于固定所述样本处理板的凹槽;The main body plate is provided with a groove for fixing the sample processing plate;

所述透镜通过所述主体板固定在所述凹槽的下方。The lens is fixed under the groove through the main body plate.

可选地,所述主体板沿竖直方向设有与所述凹槽连通的锥状通孔,所述透镜为双凸球面透镜,所述双凸球面透镜固定在所述通孔内,所述通孔沿竖直方向逐渐缩径。Optionally, the main body plate is provided with a conical through hole in a vertical direction that communicates with the groove, the lens is a biconvex spherical lens, and the biconvex spherical lens is fixed in the through hole, so The through hole is gradually reduced in diameter along the vertical direction.

可选地,所述通孔的大径端的直径为3~8mm;所述通孔的小径端的直径为0.3~0.8mm;所述通孔的沿轴向方向的高度为1~5mm。Optionally, the diameter of the large-diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm; and the height of the through hole in the axial direction is 1-5 mm.

优选地,所述双凸球面透镜的直径范围为0.5~6mm。Preferably, the diameter of the biconvex spherical lens ranges from 0.5 to 6 mm.

可选地,所述透镜为平凸透镜,所述平凸透镜的平面端固定在所述主体板的外底面,所述平凸透镜的凸面端靠近所述细胞检测器。Optionally, the lens is a plano-convex lens, the plane end of the plano-convex lens is fixed on the outer bottom surface of the main body plate, and the convex end of the plano-convex lens is close to the cell detector.

可选地,所述平凸透镜沿竖直方向的厚度为0.05-2mm,所述平凸透镜的直径范围为0.3~6mm;所述主体板厚度为1~5mm。Optionally, the thickness of the plano-convex lens in the vertical direction is 0.05-2 mm, the diameter of the plano-convex lens is 0.3-6 mm, and the thickness of the main body plate is 1-5 mm.

可选地,所述血细胞检测器还包括壳体、上盖板和无线发射器;Optionally, the blood cell detector further includes a housing, an upper cover and a wireless transmitter;

所述壳体的上表面设有下凹的用于放置所述放大计数板的样品台;The upper surface of the casing is provided with a concave sample stage for placing the magnifying counting plate;

所述样品台上设有与所述放大计数板中的透镜相配适的透光孔;The sample stage is provided with a light-transmitting hole matched with the lens in the magnifying counter;

所述图像传感器和所述无线发射器位于所述壳体围合形成的空腔内,所述图像传感器与所述无线发射器电连接;The image sensor and the wireless transmitter are located in the cavity enclosed by the casing, and the image sensor is electrically connected to the wireless transmitter;

所述图像传感器与所述透光孔相配适;the image sensor is matched with the light-transmitting hole;

所述上盖板位于所述壳体的上表面,所述上盖板的朝向所述壳体的一面安装有光源。The upper cover plate is located on the upper surface of the casing, and a light source is installed on the side of the upper cover plate facing the casing.

可选地,所述血细胞分析仪还包括采血器,所述采血器包括管体、封塞、自动定量采血管、加样管和密封套;Optionally, the blood cell analyzer further includes a blood collection device, and the blood collection device includes a tube body, a plug, an automatic quantitative blood collection tube, a sample addition tube and a sealing sleeve;

所述管体沿轴向的一端开口,所述封塞可拆卸地安装在所述管体的开口端;One end of the pipe body along the axial direction is open, and the sealing plug is detachably mounted on the open end of the pipe body;

所述自动定量采血管与所述加样管沿轴向分别固定在所述封塞的两侧,且所述自动定量采血管位于所述管体内;The automatic quantitative blood collection tube and the sample adding tube are respectively fixed on both sides of the sealing plug in the axial direction, and the automatic quantitative blood collection tube is located in the tube body;

所述自动定量采血管与所述加样管连通;The automatic quantitative blood collection tube is communicated with the sample adding tube;

所述密封套安装在所述加样管的一端。The sealing sleeve is installed at one end of the sample adding tube.

优选地,所述血细胞分析仪还包括数据分析仪,所述数据分析仪包括数据接收器、数据处理器以及显示屏;Preferably, the blood cell analyzer further includes a data analyzer, and the data analyzer includes a data receiver, a data processor and a display screen;

所述数据接收器接收所述无线发射器中的数据信号;the data receiver receives the data signal in the wireless transmitter;

所述数据接收器与所述数据处理器电连接;the data receiver is electrically connected to the data processor;

所述数据处理器与所述显示屏电连接。The data processor is electrically connected to the display screen.

本申请中,“通孔的大径端的直径”,是指锥状通孔的内径的最大值;In this application, "the diameter of the large diameter end of the through hole" refers to the maximum value of the inner diameter of the tapered through hole;

“通孔的小径端的直径”,是指锥状通孔的内径的最小值。"The diameter of the small diameter end of the through hole" refers to the minimum value of the inner diameter of the tapered through hole.

本申请能产生的有益效果包括:The beneficial effects that this application can produce include:

(1)本申请提供的血细胞分析仪,是一种无线手持式智能血细胞分析仪。血细胞镜检是血液临床检验的金标准,本申请中的计数放大板凹槽的中央区域的透镜是一个微米级的多规格光学放大镜,放大倍数从50倍到500倍不等,放大倍数由透镜的直径和焦距决定,它是一种已设定视野、焦距、放大倍数、分辨率,能够代替生物显微镜使用而且是抛弃式一次性使用的放大镜,符合临床检验标准、保证了结果的可靠性性。这种方式在人工法及仪器法血液细胞检测方法中是没有使用的。(1) The blood cell analyzer provided in this application is a wireless handheld intelligent blood cell analyzer. Blood cell microscopy is the gold standard for blood clinical tests. The lens in the central area of the groove of the counting magnification plate in this application is a micron-level multi-standard optical magnifying glass. The magnification ranges from 50 times to 500 times. The magnification is determined by the lens. It is determined by the diameter and focal length. It is a set of field of view, focal length, magnification, and resolution. It can be used instead of biological microscopes and is a disposable one-time-use magnifying glass. . This method is not used in manual and instrumental blood cell detection methods.

(2)无绳、无线化,掌中手持式,利用灵活的移动终端安装软件分析结果,因此在整个产品结构设计上,精致小巧的血细胞检测器与手机等移动终端连用,可置于手中或衣袋且不受电源限制、电磁干扰及温度湿度影响,达到移动互联网+诊断的目的,这种组成结构在目前人工法和仪器法的细胞检测中是没有使用的。(2) Cordless, wireless, hand-held in the palm, using a flexible mobile terminal to install software to analyze the results, so in the design of the entire product structure, the delicate and small blood cell detector is used in conjunction with mobile terminals such as mobile phones, and can be placed in the hand or pocket. It is not affected by power supply limitation, electromagnetic interference and temperature and humidity, and achieves the purpose of mobile Internet + diagnosis. This kind of structure is not used in the current cell detection by manual method and instrument method.

(3)智能型操作,非专业人员可以使用,加上便携、即时、可靠、互联网化等特点,故能适合多场景使用,如疾控应急、事故灾害救援、野战医疗、特殊人群上门医疗、村卫生所和社区卫生站常规检验、医院急诊、医院其它科室急用、家用自检等,突破了传统的人工法和仪器法只能在实验室固定使用的局限,改变了医学检验的场景模式,迎合互联网+医疗的政策,为应急救援、移动医疗、常见发热病人家用预检及抗生素管理等带来极大社会的价值。这种使用场景的改变而带来的社会价值在目前人工法和仪器法的血细胞检测中是没有实现的。(3) Intelligent operation, which can be used by non-professionals, plus the characteristics of portability, real-time, reliability, and Internet, so it can be used in multiple scenarios, such as disease control emergency, accident disaster rescue, field medical treatment, door-to-door medical treatment for special groups, Routine inspections in village clinics and community health stations, hospital emergency, emergency use in other departments of the hospital, and home self-inspection, etc., break through the limitation of traditional manual methods and instrument methods that can only be used in laboratories, and change the scene mode of medical inspection. Catering to the Internet + medical policy, it brings great social value to emergency rescue, mobile medical care, home pre-examination of common fever patients, and antibiotic management. The social value brought by this change of usage scenarios is not realized in the current blood cell detection by manual and instrumental methods.

附图说明Description of drawings

图1(a)为本申请一种实施方式中的样品处理板的俯视图;1(a) is a top view of a sample processing plate in an embodiment of the present application;

图1(b)为本申请一种实施方式中的样品处理板的侧视图;Figure 1(b) is a side view of a sample processing plate in an embodiment of the present application;

图2(a)为本申请第一种实施方式中的放大计数板的侧视图;Figure 2(a) is a side view of the enlarged counter board in the first embodiment of the application;

图2(b)为本申请第一种实施方式中的放大计数板的俯视图;Fig. 2(b) is a top view of the magnifying counter board in the first embodiment of the application;

图2(c)为本申请第二种实施方式中的放大计数板的侧视图;Figure 2(c) is a side view of the enlarged counter board in the second embodiment of the application;

图2(d)为本申请第二种实施方式中的放大计数板的俯视图;FIG. 2(d) is a top view of the enlarged counter board in the second embodiment of the present application;

图3(a)为本申请一种实施方式中的采血器的结构示意图;Figure 3 (a) is a schematic structural diagram of a blood collection device in an embodiment of the application;

图3(b)为本申请一种实施方式中的采血器的第一使用状态图;Fig. 3(b) is a first use state diagram of the blood sampler in an embodiment of the application;

图3(c)为本申请一种实施方式中的采血器的第二使用状态图;Fig. 3(c) is a second use state diagram of the blood sampler in an embodiment of the application;

图4为本申请一种实施方式中的血细胞检测器的结构图;4 is a structural diagram of a blood cell detector in an embodiment of the present application;

图5为本申请一种实施方式中的血细胞检测器的内部结构示意图;5 is a schematic diagram of the internal structure of a blood cell detector in an embodiment of the present application;

图6为本申请一种实施方式中的数据分析仪的结构示意图。FIG. 6 is a schematic structural diagram of a data analyzer in an embodiment of the present application.

部件和附图标记列表:List of parts and reference numbers:

101放大计数板; 1011主体板; 1012透镜;101 Magnifying and counting board; 1011 Main body board; 1012 Lens;

1013凹槽; 1014通孔;1013 groove; 1014 through hole;

102样品处理板; 1021载玻片 1022样品池;102 sample processing plate; 1021 glass slide 1022 sample cell;

1023支撑住; 1024盖玻片;1023 support; 1024 cover glass;

200采血器; 201管体; 202封塞;200 blood collection device; 201 tube body; 202 sealing plug;

203自动定量采血管; 204加样管; 205密封套;203 automatic quantitative blood collection tube; 204 sample tube; 205 sealing sleeve;

300血细胞检测器; 301壳体; 302图像传感器;300 blood cell detector; 301 housing; 302 image sensor;

303无线发射器; 304上盖板; 305样品台;303 wireless transmitter; 304 upper cover; 305 sample stage;

306透光孔; 307光源;306 light-transmitting holes; 307 light sources;

400数据分析仪; 401显示屏;400 data analyzer; 401 display screen;

1金属封闭薄膜; 2试剂管;1 metal sealing film; 2 reagent tube;

具体实施方式Detailed ways

下面结合实施例详述本申请,但本申请并不局限于这些实施例。The present application will be described in detail below with reference to the examples, but the present application is not limited to these examples.

一种血细胞分析仪,血细胞分析仪包括样本处理板、放大计数板和血细胞检测器;样本处理板包括载玻片、样本池、支持柱、盖玻片;放大计数板包括用于放置样本处理板的凹槽以及在凹槽中央的锥形通孔里固定的双凸球面透镜或固定在主体板底部一体式向外凸的平凸透镜;血细胞检测器包括图像传感器;样本处理板可拆卸地放置在放大计数板相配适的凹槽里;放大计数板可拆卸地安装到血细胞检测器中,以使经过透镜放大的血细胞样本的光学图像传输给图像传感器。A blood cell analyzer, the blood cell analyzer includes a sample processing board, a magnifying counting board and a blood cell detector; the sample processing board includes a slide glass, a sample pool, a support column, and a cover glass; the magnifying counting board includes a sample processing board for placing The groove and the biconvex spherical lens fixed in the conical through hole in the center of the groove or the integral convex plano-convex lens fixed on the bottom of the main body plate; the blood cell detector includes an image sensor; the sample processing board is detachably placed in the The magnifying counter is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample magnified by the lens is transmitted to the image sensor.

本申请所提供的血细胞分析仪,由样本处理板对样本进行流控分布处理,通过放大计数对血细胞样本进行放大,之后将放大后的光学图像传输给图像传感器。样本处理板和放大计数板可拆卸地安装到血细胞检测器中,可将检测后的样本放大计数组件和放大计数板丢弃,实现了血细胞分析一次性使用的效果,保证了测试结果的可靠性。In the blood cell analyzer provided by the present application, the sample processing board performs fluid control distribution processing on the sample, and the blood cell sample is amplified by amplification and counting, and then the amplified optical image is transmitted to the image sensor. The sample processing plate and the magnifying counting plate are detachably installed in the blood cell detector, and the sample magnifying counting assembly and the magnifying counting plate after detection can be discarded, realizing the effect of one-time use of blood cell analysis and ensuring the reliability of the test results.

可选地,样本处理板包括载玻片、样本池、支撑件、盖玻片,盖玻片沿水平方向固定在凹槽内,盖玻片与凹槽的底面之间通过支撑件形成用于盛放血细胞样本的间隙;放大计数板的中间区域设有和样本处理板相配适的凹槽,在与凹槽中央区域对应的主体板上设有锥形通孔且大径在凹槽端,在锥形通孔里固定双凸球面透镜;或在与凹槽中央区域相应主体板的底面设有一体式露出的平凸透镜;在放大计数板的凹槽里放置样本处理板。Optionally, the sample processing plate includes a slide glass, a sample cell, a support, and a cover glass, the cover glass is fixed in the groove in a horizontal direction, and a support is formed between the cover glass and the bottom surface of the groove for The gap for holding blood cell samples; the middle area of the magnifying counting plate is provided with a groove that matches the sample processing plate, and the main body plate corresponding to the central area of the groove is provided with a conical through hole and the major diameter is at the end of the groove, A biconvex spherical lens is fixed in the conical through hole; or an integrally exposed plano-convex lens is arranged on the bottom surface of the main body plate corresponding to the central area of the groove; a sample processing plate is placed in the groove of the magnifying counter.

具体地,样本处理板的支撑件可以为多个支撑柱,支撑柱的一端固定在样品池的底面,支撑柱的另一端与盖玻片固定,在盖玻片与样品池的底面之间形成了用于盛放血液样本的间隙,该间隙使得血液样本平铺成单细胞层状,从而防止细胞堆叠影响成像效果和数据分析的准确性。在放大计数板的中间区域设有凹槽,凹槽的形状大小和样本处理板相配适,放大计数板可以有两种,一种是与凹槽中央区域对应的主体板上沿竖直方向设有锥形通孔,大径在凹槽端,形成倒三角形状的锥形通孔,在锥形通孔内固定双凸球面透镜;该通孔设计为倒三角锥形状,其作用是:一方面可以支撑双凸球面透镜以便固定,另一方面小径作为双凸球面透镜的光阑,光阑的大小影响图像的亮度,光阑可以校正相差从而影响图像的分辨率,透镜的直径、焦距决定放大倍数。放大计数板的另一种是在与凹槽中央区域对应的主体板的底面上设有一体式的平凸透镜,平凸透镜的直径、焦距决定放大倍数和分辨率,该平凸透镜也设有光阑。Specifically, the support member of the sample processing plate can be a plurality of support columns, one end of the support column is fixed on the bottom surface of the sample cell, the other end of the support column is fixed with the cover glass, and formed between the cover glass and the bottom surface of the sample pool A gap for holding the blood sample is created, which allows the blood sample to be flattened into a single cell layer, thereby preventing cell stacking from affecting the imaging performance and the accuracy of data analysis. There is a groove in the middle area of the magnifying counting plate. The shape and size of the groove are suitable for the sample processing plate. There are two kinds of magnifying counting plates. There is a conical through hole, and the large diameter is at the end of the groove, forming a conical through hole in the shape of an inverted triangle, and a biconvex spherical lens is fixed in the conical through hole; the through hole is designed in the shape of an inverted triangular pyramid, and its functions are: 1. On the one hand, it can support the biconvex spherical lens for fixing. On the other hand, the small diameter is used as the diaphragm of the biconvex spherical lens. The size of the diaphragm affects the brightness of the image, and the diaphragm can correct the aberration to affect the resolution of the image. The diameter and focal length of the lens determine gain. Another type of magnification counting plate is to have an integrated plano-convex lens on the bottom surface of the main body plate corresponding to the central area of the groove. The diameter and focal length of the plano-convex lens determine the magnification and resolution, and the plano-convex lens is also provided with a diaphragm. .

本申请中的计数放大板其中央区域里的透镜是一个微米级的多规格光学放大镜,放大倍数从50倍到500倍不等,它是一种能够代替生物显微镜使用而且是抛弃式一次性使用的放大镜,符合临床检验标准、保证了结果的可靠性性。这种使用在人工法、仪器法血液细检查方法中没有使用过。The lens in the central area of the counting magnifying board in this application is a micron-level multi-specification optical magnifying glass, with a magnification ranging from 50 times to 500 times. It is a disposable type that can be used instead of a biological microscope and is disposable The magnifying glass conforms to clinical inspection standards and ensures the reliability of the results. This kind of use has not been used in the manual method and the instrument method blood examination method.

可选地,盖玻片与凹槽底面的间隙沿竖直方向的高度为0.05~0.12mm。该间隙高度可以使得血液样本平铺成较好的单细胞层状。Optionally, the height of the gap between the cover glass and the bottom surface of the groove along the vertical direction is 0.05-0.12 mm. The height of the gap allows the blood sample to be flattened into a better single-cell layer.

具体地,在盖玻片与凹槽的底面之间形成的用于盛放血液样本的间隙,可以使血液样品成单细胞层膜,较好地,厚度为0.1mm。间隙的厚度过小,支撑柱不容易制备,间隙的厚度过大,细胞会重合。Specifically, the gap for holding the blood sample formed between the cover glass and the bottom surface of the groove can make the blood sample into a monolayer film, preferably, the thickness is 0.1 mm. If the thickness of the gap is too small, the support column is not easy to prepare, and if the thickness of the gap is too large, the cells will overlap.

可选地,通孔的大径端的直径为3~8mm;通孔的小径端的直径为 0.3~0.8mm。Optionally, the diameter of the large diameter end of the through hole is 3 to 8 mm; the diameter of the small diameter end of the through hole is 0.3 to 0.8 mm.

具体地,通孔的小径端相当于光阑结构,光阑可以提高图像的亮度,光阑可以校正相差从而提高图像的分辨率。Specifically, the small diameter end of the through hole is equivalent to a diaphragm structure, the diaphragm can improve the brightness of the image, and the diaphragm can correct the phase difference to improve the resolution of the image.

可选地,通孔的大径端与小径端的直径比为10:1。Optionally, the diameter ratio of the large diameter end to the small diameter end of the through hole is 10:1.

可选地,通孔的沿轴向方向的高度为1~5mm。具体地,通孔的沿轴向方向的高度为1~5mm时,可满足焦距的大小范围。Optionally, the height of the through hole in the axial direction is 1˜5 mm. Specifically, when the height of the through hole in the axial direction is 1 to 5 mm, the size range of the focal length can be satisfied.

可选地,球面透镜为双凸透镜,可以满足加工制备。Optionally, the spherical lens is a biconvex lens, which can meet the processing requirements.

可选地,双凸球面透镜的直径范围为0.5~6mm。具体地,双凸球面透镜的直径范围为0.5~6mm时,可满足放大倍数50-500倍的范围。Optionally, the diameter of the biconvex spherical lens ranges from 0.5 to 6 mm. Specifically, when the diameter of the biconvex spherical lens is in the range of 0.5 to 6 mm, the magnification can be in the range of 50 to 500 times.

可选地,所述透镜为平凸透镜,所述平凸透镜的平面端固定在所述主体板的外底面,所述平凸透镜的凸面端靠近所述细胞检测器。Optionally, the lens is a plano-convex lens, the plane end of the plano-convex lens is fixed on the outer bottom surface of the main body plate, and the convex end of the plano-convex lens is close to the cell detector.

可选地,所述平凸透镜沿竖直方向的厚度为0.05-2mm,所述平凸透镜的直径范围为0.3~6mm;所述主体板厚度为1~5mm。Optionally, the thickness of the plano-convex lens in the vertical direction is 0.05-2 mm, the diameter of the plano-convex lens is 0.3-6 mm, and the thickness of the main body plate is 1-5 mm.

具体地,当平凸透镜沿竖直方向的厚度为0.05-2mm,平凸透镜的直径范围为0.3~6mm,可满足放大倍数50-500倍的范围,主体板厚度为1~ 5mm时,可满足焦距的大小范围。Specifically, when the thickness of the plano-convex lens in the vertical direction is 0.05-2 mm, the diameter of the plano-convex lens is 0.3-6 mm, which can satisfy the range of magnification of 50-500 times, and the thickness of the main body plate is 1-5 mm, the focal length can be satisfied. size range.

血细胞检测器还包括壳体、无线发射器和上盖板;壳体的上表面设有下凹的用于放置放大计数组件的样品台;样品台上设有与放大计数板上的透镜相配适的透光孔;图像传感器和无线发射器位于壳体围合形成的空腔内,图像传感器与无线发射器电连接;图像传感器与透光孔相配适;上盖板位于壳体的上表面,上盖板的朝向壳体的一面安装有光源。The blood cell detector also includes a casing, a wireless transmitter and an upper cover plate; the upper surface of the casing is provided with a concave sample stage for placing the magnifying and counting assembly; the sample stage is provided with a lens matching the magnifying and counting board. The image sensor and the wireless transmitter are located in the cavity enclosed by the casing, and the image sensor and the wireless transmitter are electrically connected; the image sensor is matched with the light-transmitting hole; the upper cover is located on the upper surface of the casing, A light source is installed on the side of the upper cover plate facing the casing.

具体地,样品台与放大计数板相互配适,在血液分析时,放大计数板插设在样品台上。Specifically, the sample stage and the magnifying counting plate are matched with each other, and during the blood analysis, the amplifying counting plate is inserted into the sample stage.

可选地,血细胞分析仪还包括采血器,采血器包括管体、封塞、自动定量采血管、加样管和密封套;管体沿轴向的一端开口,封塞可拆卸地安装在管体的一端;自动定量采血管与加样管沿轴向分别固定在封塞的两端,且自动定量采血管位于管体内;自动定量采血管与加样管连通;密封套安装在加样管的一端。该采血器结构简单,使用方便。Optionally, the blood cell analyzer further includes a blood collection device, which includes a tube body, a plug, an automatic quantitative blood collection tube, a sample addition tube and a sealing sleeve; one end of the tube body along the axial direction is open, and the sealing plug is detachably installed on the tube. One end of the body; the automatic quantitative blood collection tube and the sample addition tube are respectively fixed at both ends of the plug along the axial direction, and the automatic quantitative blood collection tube is located in the tube body; the automatic quantitative blood collection tube is connected with the sample addition tube; the sealing sleeve is installed in the sample addition tube one end. The blood sampling device has a simple structure and is convenient to use.

具体地,加样管用于将管体中的血液样本加入到放大计数组件中,密封套用于保证血液样本不受污染。Specifically, the sample adding tube is used to add the blood sample in the tube body to the amplification counting assembly, and the sealing sleeve is used to ensure that the blood sample is not contaminated.

可选地,血细胞分析仪还包括数据分析仪,数据分析仪包括数据接收器、数据处理器以及显示屏;Optionally, the blood cell analyzer further includes a data analyzer, and the data analyzer includes a data receiver, a data processor and a display screen;

数据接收器接收无线发射器中的数据信号;The data receiver receives the data signal from the wireless transmitter;

数据接收器与数据处理器电连接;The data receiver is electrically connected with the data processor;

数据处理器与显示屏电连接。The data processor is electrically connected to the display screen.

本申请所提供的一种新型智能可移动的血细胞分析仪,通过预先消毒好存放在密封塑料管(采血器的管体)内的定量毛细吸管(自动定量采血管),吸取手指血,然后放入另一个预先加入试剂的塑料管(如图3(c) 所示)内,混匀后滴入样本处理板的样品池中,将样本处理板放入放大计数板凹槽里,将放大计数板放入血细胞检测器内,血细胞检测器内光源灯发出的光线透过样本和放大计数板,经过放大计数板下部的透镜放大成像的血细胞光学图像,投射到光电传感器(即图像传感器)的感光面上,然后光学图像经过光电传感器及A/D转换变为数字信号,通过由wifi无线发射器传输到移动终端的数据分析仪。血细胞图像实时显示于数据分析仪的屏幕上,同时通过分析仪内的图像算法分析软件,即时分析出血细胞的数量、形态和对白细胞的分类数据,数据可以存储在分析仪内、可以无线传输到特定的数据终端、也可以通过无线打印机打印报告,分析结果实时上传到医疗互联网云平台。A new type of intelligent mobile blood cell analyzer provided by the present application absorbs finger blood through a quantitative capillary pipette (automatic quantitative blood collection tube) stored in a sealed plastic tube (the tube body of the blood collection device), which is pre-sterilized, and then put into into another plastic tube pre-added with reagents (as shown in Figure 3(c)), and after mixing, drop into the sample pool of the sample processing plate, put the sample processing plate into the groove of the magnifying counting plate, and the magnifying count The plate is placed in the blood cell detector, the light emitted by the light source lamp in the blood cell detector passes through the sample and the magnifying counting plate, and the optical image of the blood cells is enlarged and imaged through the lens at the lower part of the magnifying counting plate, and is projected to the photosensitive sensor (ie, the image sensor). Then, the optical image is converted into a digital signal through photoelectric sensor and A/D conversion, and transmitted to the data analyzer of the mobile terminal by the wifi wireless transmitter. The blood cell image is displayed on the screen of the data analyzer in real time. At the same time, through the image algorithm analysis software in the analyzer, the number, shape and classification data of blood cells can be analyzed in real time. The data can be stored in the analyzer and can be wirelessly transmitted to Specific data terminals can also print reports through wireless printers, and the analysis results can be uploaded to the medical Internet cloud platform in real time.

下面介绍可能的实现方式Possible implementations are described below

一种无线手持式智能血细胞分析仪,使用采血器200,采取手指血,放入预先加注好试剂管2中,混匀后注入样本处理板102中,将放大计数板101放到血细胞检测器300中,通过放大计数板101中的透镜1012放大成像获取血细胞图像,通过图像传感器302将光学图像转换成电信号,电信号再经过A/D转换变为数字信号,通过无线发射器303传输到数据分析仪400,利用数据终端内安装的算法分析软件,分析出血细胞的大小、数量和形态等参数,得到血小板、红细胞、白细胞总数以及白细胞分类的结果报告,结果报告将显示和存储在数据分析仪400中,用户可以直接在数据分析仪400的显示屏401上直接读取结果。测定结果可以无线打印出报告单,可以通过Wifi传输到其它特定终端,可以实时上传到医疗互联网云平台,提供临床医生和共享数据中心使用。以上血细胞检测器300为无绳无线的掌中手持式,数据分析仪400可以是各种移动终端,如手机、平板电脑、笔记本电脑、移动微型电脑等。A wireless hand-held intelligent blood cell analyzer, using a blood sampler 200 to collect finger blood, put it into a pre-filled reagent tube 2, mix it well and inject it into a sample processing board 102, and put the magnifying counting board 101 into the blood cell detector. In 300, a blood cell image is obtained by magnifying and imaging the lens 1012 in the magnifying counting board 101, the optical image is converted into an electrical signal by the image sensor 302, and the electrical signal is converted into a digital signal through A/D conversion, and is transmitted to the wireless transmitter 303. The data analyzer 400 uses the algorithm analysis software installed in the data terminal to analyze the parameters such as the size, number and shape of blood cells, and obtains the result report of the total number of platelets, red blood cells, white blood cells and white blood cell classification, and the result report will be displayed and stored in the data analysis. In the instrument 400 , the user can directly read the results on the display screen 401 of the data analyzer 400 . The measurement results can be printed out wirelessly, and can be transmitted to other specific terminals through Wifi, and can be uploaded to the medical Internet cloud platform in real time for clinicians and shared data centers. The above blood cell detector 300 is a cordless and wireless handheld handheld, and the data analyzer 400 can be various mobile terminals, such as mobile phones, tablet computers, notebook computers, mobile microcomputers, and the like.

优选地,采血器200,由塑料管(即管体201)和塑料封塞202配合组成,在封塞202下面安装一个自动定量采血管203,其容量为0.5-30ul,在塑料管帽顶部连接一个加样管204,加样管204顶部安装一个一端密封的塑料密封套205,自动定量采血管203的材料是玻璃或塑料,自动定量采血管203可以独立安装在封塞202上,自动定量采血管203也可以使用注塑工艺与封塞202一体成型。Preferably, the blood collection device 200 is composed of a plastic tube (ie, the tube body 201) and a plastic sealing plug 202. An automatic quantitative blood collection tube 203 is installed under the sealing plug 202, and its capacity is 0.5-30 ul, which is connected to the top of the plastic tube cap. A sample addition tube 204, a plastic sealing sleeve 205 sealed at one end is installed on the top of the sample addition tube 204, the material of the automatic quantitative blood collection tube 203 is glass or plastic, the automatic quantitative blood collection tube 203 can be independently installed on the plug 202, and the automatic quantitative blood collection tube 203 can be independently installed on the plug 202. The blood vessel 203 may also be integrally formed with the plug 202 using an injection molding process.

优选地,塑料管(即管体201)中预先加入稀释液、溶血素、染色剂等试剂,其管口用金属封闭薄膜1封闭。Preferably, reagents such as diluent, hemolysin, and dye are pre-added to the plastic tube (ie, the tube body 201 ), and the opening of the tube is sealed with a metal sealing film 1 .

优选地,样本处理板102用塑料注塑而成,样本处理板102包括载玻片1021,载玻片1021上部有一凹形样本池1022,在样本池1022有四个 0.1mm高度的支撑柱1023,在样本池1022底部中央区域刻有识别不同面积的虚拟细线,具体地来说,在光学放大传输后的电子图像上由软件绘制,形状为长方形,作为血液样本细胞计数的有效范围。在支撑柱1023上面固定有盖玻片1024。放大计数板101包括主体板1011,主体板1011的上表面形成凹槽1013,与凹槽1013中央区域对应的主体板1011有一个锥形小孔(即通孔1014),其孔径范围为0.3-3mm,在小孔中间放置透镜1012,透镜1012直径范围为0.5-6mm,透镜材料可以为玻璃、塑料或其他透明材料,透镜可以是球面,球面透镜有双凸、平凸。为防止细胞交叉污染和病原体交叉感染,也方便多场景现场即时使用,放大计数板101设计为多种规格的一次性使用件。Preferably, the sample processing plate 102 is made of plastic by injection molding, the sample processing plate 102 includes a glass slide 1021, the upper part of the glass slide 1021 has a concave sample pool 1022, and the sample pool 1022 has four support columns 1023 with a height of 0.1 mm, Virtual thin lines for identifying different areas are engraved in the central area of the bottom of the sample cell 1022. Specifically, they are drawn by software on the electronic image after optical magnification and transmission. A cover glass 1024 is fixed on the support column 1023 . The magnifying counter board 101 includes a main body plate 1011, a groove 1013 is formed on the upper surface of the main body plate 1011, and the main body plate 1011 corresponding to the central area of the groove 1013 has a small conical hole (namely a through hole 1014), the diameter of which is in the range of 0.3- 3mm, the lens 1012 is placed in the middle of the small hole, the diameter of the lens 1012 is 0.5-6mm, the lens material can be glass, plastic or other transparent materials, the lens can be spherical, and the spherical lens has biconvex and planoconvex. In order to prevent cross-contamination of cells and cross-infection of pathogens, and to facilitate instant use in multiple scenarios, the magnifying counting plate 101 is designed as a single-use piece of various specifications.

优选地,血细胞检测器300,其上盖板304的光源307照射置于样品处理板102中的盖玻片1024下的血液样本中细胞,通过透镜1012放大成像,放大倍数5倍~500倍,获得细胞的光学图像投射到图像传感器302 感光面上,然后光学图像通过传感器转换成电信号,电信号再经过A/D转换变为数字信号,通过无线发射器303送到数据分析仪400中进行处理,最终转换成数字图像显示在移动终端—数据分析仪400的触摸屏幕(即显示屏401)上,通过算法软件进行医学数据处理分析。Preferably, in the blood cell detector 300, the light source 307 of the upper cover plate 304 illuminates the cells in the blood sample placed under the cover glass 1024 in the sample processing plate 102, and the image is magnified by the lens 1012, and the magnification is 5 times to 500 times, The obtained optical image of the cell is projected onto the photosensitive surface of the image sensor 302, and then the optical image is converted into an electrical signal through the sensor, and the electrical signal is converted into a digital signal through A/D conversion, and sent to the data analyzer 400 through the wireless transmitter 303 for processing. After processing, it is finally converted into a digital image and displayed on the touch screen (ie, the display screen 401 ) of the mobile terminal-data analyzer 400 , and the medical data processing and analysis are performed through algorithm software.

优选地,图像传感器302,采用CCD或CMOS芯片。Preferably, the image sensor 302 adopts a CCD or a CMOS chip.

优选地,无线发射器303,采用wifi或蓝牙模块。Preferably, the wireless transmitter 303 adopts a wifi or bluetooth module.

优选地,数据分析仪400采用移动终端,可以是手机、平板电脑、笔记本电脑、移动微型电脑等,可以windows、android系统下使用,选用 windows系统时,电脑通过安装的图像算法分析软件对细胞进行医学数据处理分析,选用安卓系统时,手机安装图像算法分析的专用App软件对细胞进行医学数据处理分析。分析结果实时上传到医疗互联网云平台。Preferably, the data analyzer 400 adopts a mobile terminal, which can be a mobile phone, a tablet computer, a notebook computer, a mobile microcomputer, etc., and can be used under the windows and android systems. When the windows system is selected, the computer analyzes the cells through the installed image algorithm analysis software. For medical data processing and analysis, when the Android system is selected, the mobile phone installs a special software for image algorithm analysis to process and analyze the medical data of the cells. The analysis results are uploaded to the medical Internet cloud platform in real time.

实施例1Example 1

如图1(a)为本实施例中的样品处理板的俯视图,图1(b)为本实施例中的样品处理板的侧视图;图2(a)为本实施例中的放大计数板的侧视图,图2(b)为本实施例中的放大计数板的俯视图,图3(a)为本实施例中的采血器的结构示意图,图3(b)为本实施例中的采血器的第一使用状态图,图3(c)为本实施例中的采血器的第二使用状态图,图4为本实施例中的血细胞检测器的结构图,图5为本实施例中的血细胞检测器的内部结构示意图,图6为本实施例中的数据分析仪的结构示意图,下面结合图1~图6对本实施例进行具体说明。Fig. 1(a) is a top view of the sample processing plate in this embodiment, Fig. 1(b) is a side view of the sample processing plate in this embodiment; Fig. 2(a) is an enlarged counting plate in this embodiment Fig. 2(b) is a top view of the enlarged counter board in this embodiment, Fig. 3(a) is a schematic structural diagram of a blood collection device in this embodiment, and Fig. 3(b) is a blood collection device in this embodiment. Fig. 3(c) is a diagram of the second usage state of the blood collection device in this embodiment, Fig. 4 is a structural diagram of the blood cell detector in this embodiment, and Fig. 5 is a diagram of the blood cell detector in this embodiment. Figure 6 is a schematic diagram of the internal structure of the blood cell detector in this embodiment. Figure 6 is a schematic diagram of the structure of the data analyzer in this embodiment. The embodiment will be described in detail below with reference to Figures 1 to 6 .

如图1(a)和图1(b)所示,血细胞分析仪包括样品处理板102(整体记作102),样品处理板102包括载玻片1021、样品池1022、4个支撑柱1023和盖玻片1024,4个支撑柱1023分别位于样品池1022的对称分布的4个边缘位置处,方状的盖玻片1024沿水平方向固定在支撑柱1023 上,盖玻片1024与样品池1022的底面之间形成用于盛放血液样本的间隙。As shown in FIGS. 1( a ) and 1 ( b ), the blood cell analyzer includes a sample processing plate 102 (referred to as 102 as a whole), and the sample processing plate 102 includes a glass slide 1021 , a sample cell 1022 , four support columns 1023 and The cover glass 1024 and the four support columns 1023 are located at four symmetrically distributed edge positions of the sample cell 1022. The square cover glass 1024 is fixed on the support column 1023 along the horizontal direction. The cover glass 1024 and the sample cell 1022 A gap for holding the blood sample is formed between the bottom surfaces of the .

如图2(a)和图2(b)所示,血细胞分析仪还包括放大计数板101 (整体记作101);放大计数板101包括主体板1011和透镜1012,在主体板1011上设有方状(也可以为圆状)的凹槽1013,凹槽1013的底面沿竖直方向设有与其连通的锥状通孔1014,透镜1012置于通孔1014中,通孔 1014的大径端靠近凹槽1013,通孔1014的小径端远离凹槽1013。As shown in Fig. 2(a) and Fig. 2(b), the blood cell analyzer further includes a magnifying and counting board 101 (referred to as 101 as a whole); the magnifying and counting board 101 includes a main body plate 1011 and a lens 1012. A square (or round) groove 1013, the bottom surface of the groove 1013 is provided with a conical through hole 1014 which communicates with it in the vertical direction, the lens 1012 is placed in the through hole 1014, and the large diameter end of the through hole 1014 Close to the groove 1013 , the small diameter end of the through hole 1014 is away from the groove 1013 .

间隙沿竖直方向的高度为0.1mm,通孔1014的大径端的直径为3mm,通孔1014的小径端的直径为0.3mm,通孔1014的沿轴向方向的高度为 2mm,透镜1012为双凸透镜,直径为0.8mm。The height of the gap in the vertical direction is 0.1 mm, the diameter of the large diameter end of the through hole 1014 is 3 mm, the diameter of the small diameter end of the through hole 1014 is 0.3 mm, the height of the through hole 1014 in the axial direction is 2 mm, and the lens 1012 is double A convex lens with a diameter of 0.8mm.

如图3(a)所示,血细胞分析仪还包括采血器200(整体记作200),采血器包括管体201、封塞202、自动定量采血管203、加样管204和密封套205;管体201沿轴向的一端开口,封塞202可拆卸地安装在管体201 的一端,形成密封效果;自动定量采血管203与加样管204沿轴向分别固定在封塞202的两端,且自动定量采血管203位于管体201内;自动定量采血管203与加样管204连通;密封套205安装在加样管204的一端。As shown in Figure 3(a), the blood cell analyzer further includes a blood collection device 200 (referred to as 200 as a whole), and the blood collection device includes a tube body 201, a plug 202, an automatic quantitative blood collection tube 203, a sample addition tube 204 and a sealing sleeve 205; One end of the tube body 201 is open in the axial direction, and the sealing plug 202 is detachably installed on one end of the tube body 201 to form a sealing effect; , and the automatic quantitative blood collection tube 203 is located in the tube body 201 ; the automatic quantitative blood collection tube 203 is communicated with the sample adding tube 204 ;

如图4和图5所示,血细胞分析仪还包括血细胞检测器300(整体记作300),血细胞检测器300包括壳体301、图像传感器302、无线发射器 303和上盖板304;壳体301的上表面设有下凹的用于放置放大计数板101 的样品台305;样品台305上设有与放大计数板101上的锥状通孔1014 相配适的透光孔306;图像传感器302和无线发射器303位于壳体301围合形成的空腔内,图像传感器302与无线发射器303电连接;图像传感器 302与透光孔306相配适;上盖板304位于壳体301的上表面,上盖板304 的朝向壳体的一面安装有光源307。As shown in FIG. 4 and FIG. 5 , the blood cell analyzer further includes a blood cell detector 300 (denoted as 300 as a whole), and the blood cell detector 300 includes a housing 301, an image sensor 302, a wireless transmitter 303 and an upper cover 304; the housing The upper surface of 301 is provided with a concave sample stage 305 for placing the magnifying counter board 101; the sample stage 305 is provided with a light-transmitting hole 306 that matches the tapered through hole 1014 on the magnifying counter board 101; the image sensor 302 The image sensor 302 and the wireless transmitter 303 are located in the cavity enclosed by the casing 301, and the image sensor 302 is electrically connected to the wireless transmitter 303; the image sensor 302 is matched with the light-transmitting hole 306; , a light source 307 is installed on the side of the upper cover plate 304 facing the casing.

如图6所示,血细胞分析仪还包括数据分析仪400,数据分析仪400 包括数据接收器、数据处理器以及显示屏401;数据接收器接收无线发射器中的数据信号;数据接收器与数据处理器电连接;数据处理器与显示屏 401电连接。As shown in FIG. 6, the blood cell analyzer further includes a data analyzer 400, and the data analyzer 400 includes a data receiver, a data processor and a display screen 401; the data receiver receives the data signal in the wireless transmitter; the data receiver and the data The processor is electrically connected; the data processor is electrically connected to the display screen 401 .

实施例2Example 2

图2(c)为本实施例中的放大计数板的侧视图,图2(d)为本实施例中的放大计数板的俯视图。下面结合图2(c)和图2(d)进行说明。FIG. 2( c ) is a side view of the enlarged counting plate in this embodiment, and FIG. 2( d ) is a top view of the enlarged counting plate in this embodiment. The following description will be made with reference to Fig. 2(c) and Fig. 2(d).

本实施例提供了另一种放大计数板101的结构,如图2(c)和图2(d) 所示,在放大计数板101的主体板1011上设有凹槽1013,与凹槽1013中央区域对应主体板1011的底面设有一体式向外凸出的平凸透镜1012。This embodiment provides another structure of the magnifying counter board 101. As shown in FIG. 2(c) and FIG. 2(d) , a groove 1013 is provided on the main body plate 1011 of the magnifying counter board 101, and the groove 1013 An integral plano-convex lens 1012 protruding outward is provided in the central area corresponding to the bottom surface of the main body plate 1011 .

本实施例中,平凸透镜沿竖直方向的厚度为0.3mm,平凸透镜的直径范围为0.5-1.0mm,主体板厚度为1.5mm。In this embodiment, the thickness of the plano-convex lens in the vertical direction is 0.3 mm, the diameter of the plano-convex lens is in the range of 0.5-1.0 mm, and the thickness of the main body plate is 1.5 mm.

下面介绍本实施例所提供的血细胞分析仪的使用方法。The following describes the use method of the blood cell analyzer provided in this embodiment.

首先,利用采血器200采血,具体操作为:用商品采血针刺破预先酒精消毒的无名指,抹去第一滴血,轻挤手指血,拧下采血器200管体201 上面的封塞202,用自动定量采血管203吸取血液,之后放入撕去金属封闭薄膜1的试剂管2内,如图3(b)所示,试剂管2的规格与采血器200 管体201相同,盖紧封塞202,如图3(c)所示,将试剂管2轻轻颠倒混匀。First, use the blood collection device 200 to collect blood. The specific operation is as follows: use a commercial blood collection needle to puncture the ring finger sterilized with alcohol in advance, wipe off the first drop of blood, squeeze the blood from the finger lightly, and unscrew the sealing plug 202 on the tube body 201 of the blood collection device 200. Use the automatic quantitative blood collection tube 203 to draw blood, and then put it into the reagent tube 2 with the metal sealing film 1 torn off, as shown in FIG. The stopper 202, as shown in Fig. 3(c), gently inverts the reagent tube 2 to mix.

然后,将混合好的血液样本(即血细胞样本)移入样品处理板102中。具体操作为:移去加样管204上面的密封套205,然后将试剂管2倒置,挤压试剂管2,弃去加样管204内的前端稀释血液,再将后续的稀释血液一滴,滴入样品处理板102的样品池1022内,至盖玻片1024下面的间隙充满稀释血液样本。Then, the mixed blood sample (ie, blood cell sample) is transferred into the sample processing plate 102 . The specific operation is: remove the sealing sleeve 205 on the sample addition tube 204, then invert the reagent tube 2, squeeze the reagent tube 2, discard the front end of the sample addition tube 204 to dilute the blood, and then add a drop of the subsequent diluted blood, drop into the sample cell 1022 of the sample processing plate 102, and the gap below the cover glass 1024 is filled with the diluted blood sample.

最后,对血液样本进行测试分析。具体操作为:将样品处理板102放入放大计数板101中的凹槽1013内,然后将放大计数板101放入血细胞检测器300中的样本台305中,将血细胞检测器300的上盖板304合上,打开血细胞检测器300的电源开关,同时打开数据分析仪400的电源开关,确认血细胞检测器300与数据分析仪400已经通过无线连接,此时在显示屏401上会显示血细胞的图像,数据分析仪400通过内置的算法分析软件自动分析出细胞数量、形态和白细胞分类结果。结果自动存储在数据分析仪400内,也可通过Wifi传输到特定的数据终端或打印出报告,分析结果实时上传到医疗互联网云平台。Finally, a blood sample is tested and analyzed. The specific operation is as follows: put the sample processing plate 102 into the groove 1013 in the magnifying counting plate 101 , then put the magnifying counting plate 101 into the sample stage 305 in the blood cell detector 300 , put the upper cover plate of the blood cell detector 300 304 is closed, turn on the power switch of the blood cell detector 300, and turn on the power switch of the data analyzer 400 at the same time, confirm that the blood cell detector 300 and the data analyzer 400 have been wirelessly connected, and the image of blood cells will be displayed on the display screen 401 at this time. , the data analyzer 400 automatically analyzes the cell number, morphology and white blood cell classification results through the built-in algorithm analysis software. The results are automatically stored in the data analyzer 400, and can also be transmitted to a specific data terminal or printed out through Wifi, and the analysis results are uploaded to the medical Internet cloud platform in real time.

以上,仅是本申请的几个实施例,并非对本申请做任何形式的限制,虽然本申请以较佳实施例揭示如上,然而并非用以限制本申请,任何熟悉本专业的技术人员,在不脱离本申请技术方案的范围内,利用上述揭示的技术内容做出些许的变动或修饰均等同于等效实施案例,均属于技术方案范围内。The above are just a few embodiments of the present application, and are not intended to limit the present application in any form. Although the present application is disclosed above with preferred embodiments, it is not intended to limit the present application. Deviating from the scope of the technical solutions of the present application, any changes or modifications made by using the technical contents disclosed above are equivalent to equivalent implementation cases and fall within the scope of the technical solutions.

Claims (10)

1. A blood cell analyzer, comprising a sample processing plate, an amplifying and counting plate, and a blood cell detector;
the sample processing plate is used for containing a blood cell sample;
the magnifying counting plate comprises a lens;
the blood cell detector includes an image sensor;
the sample processing plate is detachably mounted on the amplification counting plate, and the amplification counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample amplified by the lens is transmitted to the image sensor.
2. The hematology analyzer of claim 1, wherein the sample processing plate includes a slide, a support, and a cover slip;
a concave sample pool is arranged on the glass slide;
the cover glass and the support piece are both positioned in the sample pool, and a gap for containing the blood cell sample is formed between the cover glass and the bottom surface of the sample pool through the support piece.
3. The hematology analyzer of claim 2, wherein the height of the gap in the vertical direction is 0.05-0.12 mm.
4. The hematology analyzer of claim 1, wherein the enlarged count plate further comprises a body plate;
a groove for fixing the sample processing plate is arranged on the main body plate;
the lens is fixed below the groove through the main body plate.
5. The hematology analyzer of claim 4, wherein the main body plate is provided with a tapered through hole in vertical direction, the tapered through hole is communicated with the groove, the lens is a biconvex spherical lens, the biconvex spherical lens is fixed in the through hole, and the through hole is gradually reduced in diameter in vertical direction.
6. The hematology analyzer of claim 5, wherein the diameter of the large diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm; the height of the through hole along the axial direction is 1-5 mm;
the diameter range of the biconvex spherical lens is 0.5-6 mm.
7. The hematology analyzer of claim 4, wherein the lens is a plano-convex lens, a planar end of the plano-convex lens is fixed to an outer bottom surface of the main body plate, and a convex end of the plano-convex lens is close to the cell detector.
8. The hematology analyzer of claim 7, wherein the thickness of the plano-convex lens in the vertical direction is 0.05-2mm, and the diameter of the plano-convex lens is in the range of 0.3-6 mm; the thickness of the main body plate is 1-5 mm.
9. The blood cell analyzer of claim 1, wherein the blood cell detector further comprises a housing, an upper cover plate, and a wireless transmitter;
a concave sample table for placing the amplifying and counting plate is arranged on the upper surface of the shell;
the sample table is provided with a light hole matched with the lens in the magnifying counting plate;
the image sensor and the wireless transmitter are positioned in a cavity formed by the enclosure of the shell, and the image sensor is electrically connected with the wireless transmitter;
the image sensor is matched with the light hole;
the upper cover plate is located the upper surface of casing, the orientation of upper cover plate the light source is installed to the one side of casing.
10. The hematology analyzer of claim 1, further comprising a blood sampler, the blood sampler comprising a tube body, a plug, an automatic quantitative blood collection tube, a sample addition tube, and a sealing sleeve;
one end of the tube body along the axial direction is opened, and the sealing plug is detachably arranged at the opening end of the tube body;
the automatic quantitative blood collection tube and the sample adding tube are respectively fixed on two sides of the sealing plug along the axial direction, and the automatic quantitative blood collection tube is positioned in the tube body;
the automatic quantitative blood collection tube is communicated with the sample adding tube;
the seal sleeve is arranged at one end of the sample adding pipe;
the blood cell analyzer further comprises a data analyzer, wherein the data analyzer comprises a data receiver, a data processor and a display screen;
the data receiver receives a data signal in the wireless transmitter;
the data receiver is electrically connected with the data processor;
the data processor is electrically connected with the display screen.
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