CN211199228U - Tissue cell dissociation device with constant temperature device - Google Patents

Abstract

The utility model relates to a tissue cell dissociation device with a constant temperature device. The bottle body is put into a clamping structure of an incubator, and the clamping structure can drive the bottle body to rotate. The filling port is connected to an air bath box. The filling pipes arranged on the side walls are arranged in alignment; at least one gas inlet is arranged below the outer edge of the insulation box, at least one gas outlet is arranged above the outer edge of the insulation box or at the upper end face, and at least one gas outlet is arranged on the insulation box a temperature sensor. In the utility model, the rotation can cause the liquid in the bottle to have a corresponding stirring effect, which avoids the damage to the cells caused by the existing agitator. The tissue and tissue dissociating agent can keep the temperature of the bottle body from changing too much during the filling process, ensuring that the treatment starts and finishes under constant temperature conditions.

Description

Tissue cell dissociation device with thermostat

technical field

The utility model belongs to the technical field of tissue cell dissociation experimental equipment improvement, in particular to a tissue cell dissociation device with a constant temperature device.

Background technique

Histiocyte dissociation is to destroy the proteins that maintain the cell association in the intercellular space, so that the cells become single and separated. Specifically, the tissue cells are treated with protease, and the single cells are separated for subsequent culture. The device used is : Including a bottle body 12, a bottle cap 11 and a stirrer, the upper end of the bottle body is engaged and sleeved with a bottle cap, and a stirrer is arranged in the bottle body. When in use, put the tissue cells into the bottle body, add the cell dissociation agent through the filling port 13, and drive the stirrer set in the bottle body through the magnetic attraction through the external magnetic attraction structure. time. The above equipment has the following problems: 1. The purpose of the stirrer is to stir the tissue cells and tissue dissociating agent in the bottle to make them uniform, but during the stirring process, due to the structure of the stirring blade or blade, it will collide with the cells, thereby 2. Place the tissue in the bottle first, then add the dissociating agent, and then put it into the incubator for processing. The temperature of the bottle is low when it is just put in, and it takes a period of time to achieve constant temperature processing conditions; 3. It is more troublesome to clean the mixer.

Utility model content

The purpose of this utility model is to overcome the deficiencies of the prior art, and to provide a thermostatic device with a scientific structure, the liquid in the bottle is stirred by the rotation of the bottle itself, and the tissue and tissue dissociating agent are directly added into the preheated bottle. tissue cell dissociation device.

The technical scheme adopted by the utility model is:

A tissue cell dissociation device with a constant temperature device, comprising a bottle body and a bottle cap, the bottle cap is sleeved on the opening of the upper end of the bottle body, and a filling tube is arranged on the bottle body, characterized in that: the bottle body is characterized in that: The body is placed into the clamping structure of the incubator, and the clamping structure can drive the bottle body to rotate, and the filling port is arranged in alignment with the filling pipe provided on the side wall of the gas bath box;

At least one gas inlet is arranged below the outer edge of the insulation box, at least one gas outlet is arranged above the outer edge of the insulation box or at the upper end face, and at least one temperature sensor is arranged on the insulation box.

Furthermore, a partition is arranged at the lower end of the heat preservation box, a bearing or a bearing is arranged in the partition, a vertical rotating shaft is arranged in the bearing or bearing, and the upper end of the rotating shaft is connected to the bottom surface of the clamping structure, and the rotation The lower end of the shaft extends under the baffle and is driven by a motor.

Furthermore, the partition is provided with a drain pipe, and the lower end of the drain pipe passes through the side wall of the incubator and is located outside the incubator.

Furthermore, the heat preservation box is provided with a transparent window.

Furthermore, a plurality of gas inlets are arranged on the side wall of the incubator above the partition plate, and the ends of the plurality of gas inlets located on the outside of the incubator are communicated with an annular branch pipe, and the annular branch pipe is provided with an intake manifold.

Furthermore, a plurality of gas outlets are arranged on the upper end surface of the incubator, and the ends of the plurality of gas outlets located above the incubator are communicated with the gas outlet main pipe.

Furthermore, the clamping structure includes an elastic sleeve and a rigid sleeve, the bottom surface of the rigid sleeve is connected with the upper end of the rotating shaft, the elastic sleeve is embedded in the rigid sleeve, and the lower end of the bottle body is embedded in the elastic sleeve, and many more are arranged on the outer edge of the rigid sleeve. A locking bolt whose end passes through the rigid sleeve and abuts against the outer edge of the elastic sleeve.

Furthermore, the filling tube is provided with a sealing cover.

Furthermore, a plurality of temperature sensors are arranged on the side wall of the insulation box between the gas inlet and the filling pipe, and at least one temperature sensor is arranged on the upper end surface of the insulation box.

The advantages and positive effects of the present utility model are:

In the utility model, the bottle body is placed in the clamping structure, and the rotating shaft driven by the motor drives the bottle body to rotate through the clamping structure. Destruction of cells, and the bottle body is put into an incubator in advance, and hot gas is introduced for a period of time to keep the bottle body under constant temperature conditions, and then tissue is added to the bottle body through the filling tube and filling port and tissue dissociation It can keep the temperature of the bottle body from changing too much during the filling process, ensuring that the treatment starts and finishes under constant temperature conditions.

Description of drawings

Fig. 1 is the structural representation of the present utility model;

FIG. 2 is a cross-sectional view of FIG. 1 .

Detailed ways

The present utility model will be further described below in conjunction with the examples. The following examples are illustrative, not restrictive, and the protection scope of the present utility model cannot be limited by the following examples.

A tissue cell dissociation device with a constant temperature device, as shown in Figures 1-2, includes a bottle body 12 and a bottle cap 11, the bottle cap is sleeved at the opening of the upper end of the bottle body, and a filling port is arranged on the bottle body 13. The innovation of the present utility model is: the bottle body is put into the clamping structure of the incubator 18, the clamping structure can drive the bottle body to rotate, and the filling port and the filling pipe set on the side wall of the air bath box are two pairs of pairs. bit set;

At least one gas inlet 24 is provided below the outer edge of the incubator, at least one gas outlet 5 is provided above the outer edge of the incubator or at the upper end face, and at least one temperature sensor is provided on the incubator.

In this embodiment, the thermal insulation box has a thermal insulation outer layer to reduce the dissipation of heat in the thermal insulation box. A partition 26 is arranged at the lower end of the incubator, a bearing or a bearing 25 is arranged in the partition, and a vertical rotating shaft 23 is arranged in the bearing or bearing. The upper end of the rotating shaft is connected to the bottom surface of the clamping structure, and the lower end 29 of the rotating shaft It extends under the partition and is connected to the motor 31 through the transmission mechanism 30 . The transmission mechanism can be a common deceleration transmission structure such as a gear pair, that is, the high speed deceleration and direction change of the motor is transmitted to the rotating shaft, so that it can rotate at a lower speed and drive the bottle body to rotate. The rotation speed of the rotating shaft can be continuously changed, thus forming a faster-slower-faster-slower change, so that the liquid in the bottle rotates suddenly and suddenly, which is roughly similar to the effect of vibration, which improves the resistance to vibration. The stirring ability of the liquid. The control of the above-mentioned motor can be realized by a controller, and the controller takes the CPU as the core and is in the prior art, and will not be described in detail here.

The partition plate is provided with an upper end 27 of a drain pipe 28, and the lower end 21 of the drain pipe passes through the side wall of the incubator and is located outside the incubator. The incubator is provided with a side door 7 that can be opened, and a transparent window can be provided on the side door. The transparent window can be composed of double-layer glass, and a vacuum is drawn between the two layers of glass to reduce the heat dissipation in the incubator. A plurality of gas inlets are arranged on the side wall of the incubator above the clapboard, and the ends of the plurality of gas inlets located on the outside of the incubator are communicated with the annular branch pipe 19, and the annular branch pipe is provided with an intake manifold 20, and heat is introduced into the intake manifold. gas. A plurality of gas outlets are arranged on the upper end surface of the incubator, and the ends of the plurality of gas outlets located above the incubator are communicated with the gas outlet pipe 10, and the gas outlet pipe discharges the cooled gas. The hot gas can be heated air or, under certain test conditions, heated inert gas. The hot gas can be a serpentine tube that directly introduces air or inert gas into the external winding heating resistance wire. After being heated in the serpentine tube, it flows to the intake manifold through the insulation pipeline, and finally the temperature in the insulation box is 36-37 ℃. Celsius.

The clamping structure includes an elastic sleeve 15 and a rigid sleeve 16, the bottom surface of the rigid sleeve is connected to the upper end of the rotating shaft, the elastic sleeve is embedded in the rigid sleeve, the lower end of the bottle body is embedded in the elastic sleeve, and a plurality of ends are arranged on the outer edge of the rigid sleeve to pass through the rigid sleeve. Locking bolts 17 that cover and push against the outer edge of the elastic sleeve. Since the rotation speed of the bottle body is low, and the rubber of the elastic sleeve can be squeezed and deformed, the locking bolts can be tightened to a certain degree in advance, and then the lower end of the bottle body can be squeezed into the elastic sleeve when using.

The filling pipe is provided with a sealing cover 1, which is opened during filling, and then tightened and closed, which can be provided with a heat insulation layer to function as sealing and heat preservation. A plurality of temperature sensors 14 are arranged on the side wall of the insulation box between the gas inlet and the filling pipe, and at least one temperature sensor 8 is arranged on the upper end surface of the insulation box.

The upper end surface of the incubator is actually an openable structure, which includes a base plate 3 and an elastic part 4. The gas outlet and the temperature sensor are penetrated on the base plate and the elastic part, and the elastic part can squeeze into the upper end of the incubator after entering the upper end of the incubator. edge, so that the place is closed and insulated.

The output ends 6 of all temperature sensors are connected to the controller, and the controller determines whether the injection speed of the hot gas needs to be increased or decreased, and the controller realizes the change of the gas flow through the air pump.

In the utility model, the bottle body is placed in the clamping structure, and the rotating shaft driven by the motor drives the bottle body to rotate through the clamping structure. Destruction of cells, and the bottle body is put into the incubator in advance, and hot gas is introduced for a period of time to keep the bottle body under constant temperature conditions, and then tissue is added to the bottle body and tissue dissociation through the filling tube and filling port. It can keep the temperature of the bottle body from changing too much during the filling process, ensuring that the treatment starts and finishes under constant temperature conditions.

Claims (9)

1. a tissue cell dissociation device with a constant temperature device, comprising a bottle body and a bottle cap, the bottle cap is sleeved on the bottle body upper end opening, and the bottle body is provided with a filling port, it is characterized in that: The bottle body is put into the holding structure of the incubator, and the holding structure can drive the bottle body to rotate, and the filling port is arranged in alignment with the filling pipe provided on the side wall of the gas bath box; At least one gas inlet is arranged below the outer edge of the insulation box, at least one gas outlet is arranged above the outer edge of the insulation box or at the upper end face, and at least one temperature sensor is arranged on the insulation box. 2 . The tissue cell dissociation device with a constant temperature device according to claim 1 , wherein a partition is arranged at the lower end of the incubator, and a bearing or a bearing bush is arranged in the partition. A vertical rotating shaft is arranged inside, the upper end of the rotating shaft is connected to the bottom surface of the clamping structure, and the lower end of the rotating shaft extends under the partition plate and is driven by the motor. 3 . The tissue cell dissociation device with a constant temperature device according to claim 2 , wherein the partition is provided with a drain pipe, and the lower end of the drain pipe passes through the side wall of the incubator and is located in the incubator 3 . outside. 4 . The tissue cell dissociation device with a constant temperature device according to claim 3 , wherein the incubator is provided with a transparent window. 5 . 5 . The tissue cell dissociation device with a thermostat according to claim 4 , wherein a plurality of gas inlets are arranged on the side wall of the incubator above the partition plate, and the plurality of gas inlets are located outside the incubator. 6 . The end of the air inlet is communicated with the annular branch pipe, and the annular branch pipe is provided with an intake manifold. 6 . The tissue cell dissociation device with a constant temperature device according to claim 5 , wherein a plurality of gas outlets are arranged on the upper end surface of the incubator, and the plurality of gas outlets are located at the end above the incubator and the gas outlet. 7 . The main pipe is connected. 7. A tissue cell dissociation device with a constant temperature device according to claim 2 or 3 or 4 or 5 or 6, wherein the clamping structure comprises an elastic sleeve and a rigid sleeve, and the bottom surface of the rigid sleeve and the The upper end of the rotating shaft is connected, an elastic sleeve is embedded in the rigid sleeve, and the lower end of the bottle body is embedded in the elastic sleeve. 8 . The tissue cell dissociation device with a constant temperature device according to claim 7 , wherein the filling tube is provided with a sealing cover. 9 . 9 . The tissue cell dissociation device with a constant temperature device according to claim 8 , wherein a plurality of temperature sensors are arranged on the side wall of the incubator between the gas inlet and the filling pipe, and the upper end surface of the incubator Set at least one temperature sensor.

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