CN211170710U - Conveying device and culture system for 3D (three-dimensional) organoid - Google Patents
Conveying device and culture system for 3D (three-dimensional) organoid Download PDFInfo
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- CN211170710U CN211170710U CN201921838029.1U CN201921838029U CN211170710U CN 211170710 U CN211170710 U CN 211170710U CN 201921838029 U CN201921838029 U CN 201921838029U CN 211170710 U CN211170710 U CN 211170710U
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- plate
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- organoid
- culture plate
- hole
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- 210000002220 organoid Anatomy 0.000 title claims abstract description 57
- 238000004113 cell culture Methods 0.000 claims abstract description 103
- 230000007246 mechanism Effects 0.000 claims abstract description 9
- 210000001503 joint Anatomy 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 4
- 238000002955 isolation Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000000560 biocompatible material Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920002529 medical grade silicone Polymers 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model relates to a conveyer and cultivation system of 3D organoid, this conveyer includes: a base plate; a cell culture plate provided on the bottom plate, the cell culture plate having a plurality of cell culture wells; the cover plate is arranged at the top of the cell culture plate and is detachably connected with the cell culture plate, a hollow hole corresponding to the cell culture hole is formed in the cover plate, and a ventilating piece used for separating the cell culture hole from the outside is arranged between the hollow hole and the cell culture hole; and a fixing mechanism for fixing the base plate, the cell culture plate and the cover plate integrally. The utility model discloses a conveyer can directly carry out the batch transportation in placing the cell culture board with 3D organoid in, and 3D organoid and external isolation can be guaranteed to ventilative piece to prevent the adhesion between the 3D organoid, guarantee the cultivation effect of 3D organoid.
Description
Technical Field
The utility model relates to a bioengineering uses technical field, concretely relates to conveyer and cultivation system of 3D organoid.
Background
3D organoids show great potential in many applications including disease modeling and regenerative medicine. 3D cell models, such as 3D organoids and spheroids, give us the opportunity to better understand the complexity of biology in physiologically relevant contexts relative to 2D models. However, the culture period of 3D organoids is long and the culture process is cumbersome. Currently there is no specific device available for 3D organoid transport. The existing transportation method is to collect a plurality of 3D organoids in a sealed container filled with culture medium for transportation. However, in the process of taking out the 3D organoids from the culture medium and transferring the 3D organoids to the culture plate again, adhesion occurs between a plurality of 3D organoids, and the 3D organoids are easily damaged when being taken and placed, which affects the culture and growth state of the 3D organoids in the later period.
SUMMERY OF THE UTILITY MODEL
In view of the above problems in the prior art, it is an object of the present invention to provide a 3D organoid transporter, which can solve at least the above problems in the prior art.
In order to achieve the above object, the embodiment of the present invention provides a transportation device for 3D organoids, including:
a base plate;
a cell culture plate provided on the bottom plate, the cell culture plate having a plurality of cell culture wells;
the cover plate is arranged at the top of the cell culture plate and is detachably connected with the cell culture plate, a hollow hole corresponding to the cell culture hole is formed in the cover plate, and a ventilating piece used for separating the cell culture hole from the outside is arranged between the hollow hole and the cell culture hole;
and a fixing mechanism for fixing the base plate, the cell culture plate and the cover plate integrally.
In some embodiments, the cell culture plate comprises a first plate body and a first side plate bent vertically upward along an edge of the first plate body; the cover plate comprises a second plate body and a second side plate which is vertically bent downwards along the edge of the second plate body, and the first side plate and the second side plate can be in butt joint and embedded.
In some embodiments, the gas permeable member is a unitary structure disposed between the cell culture plate and the cover plate, the gas permeable member being sized to match the size of the cell culture plate.
In some embodiments, the air permeable member is a breathable medical silicone layer.
In some embodiments, the securing mechanism comprises a snap.
In some embodiments, the edge of the bottom plate is provided with a limiting foot for limiting the cell culture plate.
In some embodiments, a backing plate is further disposed between the cell culture plate and the bottom plate.
In some embodiments, the cell culture wells are equispaced on the cell culture plate.
In some embodiments, the cell culture plate is a standard cell culture plate.
The embodiment of the utility model provides a culture system of 3D organoid still is provided, including foretell 3D organoid's conveyer.
Compared with the prior art, the embodiment of the utility model provides a 3D organoid's conveyer passes through the bottom plate that fixed establishment will connect gradually, cell culture board and apron are fixed in an organic wholely, and will be used for placing the cell culture hole of 3D organoid (or it connects the ball) through ventilative piece and external separation, can directly carry out the bulk transport in the cell culture board with 3D organoid is arranged in, ventilative piece is when guaranteeing gas exchange, avoid the transport in the way because of jolting and cause the cross condition of liquid between the cell culture hole, guarantee downthehole 3D organoid of cell culture and external isolation, be in aseptic environment, and prevent adhesion between each 3D organoid, guarantee 3D organoid's cultivation effect. In addition, after all parts of the transportation device are used, the transportation device can be repeatedly used after being cleaned and sterilized, so that the use cost is saved, and the transportation device is energy-saving and environment-friendly.
Drawings
In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments, by way of example and not by way of limitation, and together with the description and claims, serve to explain the embodiments of the invention. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.
Fig. 1 is a schematic view of an assembly structure of a 3D organoid transporter according to an embodiment of the present invention;
fig. 2 is an exploded view of a 3D organoid transporter in accordance with an embodiment of the present invention;
fig. 3 is a top view of a base plate according to an embodiment of the present invention;
fig. 4 is a schematic structural view of the buckle according to the embodiment of the present invention.
Reference numerals:
1-bottom plate, 11-spacing foot;
2-cell culture plate, 201-cell culture hole, 21-first plate body, 22-first side plate;
3-cover plate, 301-hollow hole, 31-second plate body and 32-second side plate;
4-a gas permeable member; 5-a fastener; 6-backing plate.
Detailed Description
In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined below to clearly and completely describe the technical solution of the embodiments of the present invention.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
To maintain the following description of the embodiments of the present invention clear and concise, detailed descriptions of well-known functions and components may be omitted.
Fig. 1 and 2 show a schematic structural diagram of a 3D organoid transporter according to an embodiment of the present invention. As shown in fig. 1 and 2, embodiments of the present invention provide a 3D organoid transporter, including:
a base plate 1;
a cell culture plate 2 arranged on the bottom plate 1, wherein the cell culture plate 2 is provided with a plurality of cell culture holes 201 for placing culture media of the 3D organoid;
the cover plate 3 is arranged at the top of the cell culture plate 2 and is detachably connected with the cell culture plate 2, a hollow hole 301 corresponding to the cell culture hole 201 is formed in the cover plate 3, and a ventilating piece 4 used for separating the cell culture hole 201 from the outside is arranged between the hollow hole 301 and the cell culture hole 201;
and a fixing mechanism for integrally fixing the base plate 1, the cell culture plate 2, and the cover plate 3.
The embodiment of the utility model provides a 3D organoid's conveyer passes through the bottom plate 1 that fixed establishment will connect gradually, cell culture board 2 and apron 3 are fixed in an organic whole, and will be used for placing 3D organoid (or its cell culture hole 201 that connects the seedball) through ventilative piece 4 and separate with the external world, can arrange 3D organoid in cell culture board 201 and directly carry out batch transportation, ventilative piece 4 is when guaranteeing gas exchange, avoid the transportation because of jolting and cause the cross condition of liquid between the cell culture hole 201, guarantee 3D organoid and external isolation in the cell culture hole 201, be in aseptic environment, and prevent the adhesion between each 3D organoid, guarantee 3D organoid's cultivation effect. In addition, after all parts of the transportation device are used, the transportation device can be repeatedly used after being cleaned and sterilized, so that the use cost is saved, and the transportation device is energy-saving and environment-friendly.
In some embodiments, as shown in fig. 1 and 2, the cell culture plate 2 comprises a first plate 21 and a first side plate 22 bent vertically upward along an edge of the first plate 21; the cover plate 3 comprises a second plate body 31 and a second side plate 32 which is vertically bent downwards along the edge of the second plate body 31, a cell culture hole 201 is formed in the first plate body 21, a hollow hole 301 corresponding to the cell culture hole 201 is formed in the second plate body 31, the first side plate 22 and the second side plate 32 are in butt joint and embedded, and the cover plate 3 can be covered on the top of the cell culture plate 2.
In this embodiment, the first board body 21 and the second board body 31 are rectangular boards and are disposed oppositely, and the first side board 22 and the second side board 32 are disposed oppositely and can be connected in an embedded manner. In other embodiments, the first plate 21 and the second plate 31 may be square, circular or oval, and the present invention is not limited thereto, and accordingly, the shape of the bottom plate 1 matches the shape of the cell culture plate 2.
In some embodiments, the gas permeable member 4 is a one-piece structure that is disposed between the cell culture plate 2 and the cover plate 3, and the gas permeable member 4 is sized to match the size of the cell culture plate 2. Set up ventilative piece 4 to the integral type structure, easy dismounting, the whole change of being convenient for. In addition, the cover plate 3 can press the integrated gas permeable member 4, thereby preventing the culture solution in the cell culture well 201 from leaking and flowing out. The air-permeable member 4 is preferably an air-permeable medical silica gel layer, and has good air permeability and water resistance.
In other embodiments, an individual air-permeable member 4 with a smaller size can be arranged at the top of each cell culture hole 201 to be isolated from the outside, the air-permeable material can be saved by adopting the individual air-permeable member 4, and meanwhile, the air-permeable member 4 arranged in the cell culture hole 201 can be replaced independently when damaged, and the air-permeable member 4 does not need to be replaced integrally.
In some embodiments, as shown in fig. 4, the fixing mechanism comprises a fastener 5, and the bottom plate 1, the cell culture plate 2 and the cover plate 3 combined together can be directly fixed by the fastener 5 and transported integrally. In another embodiment, the fixing mechanism may also be a bolt, a pin, or other fixing mechanism, and the bottom plate 1, the cell culture plate 2, and the cover plate 3 are provided with corresponding bolt holes or pin holes.
In some embodiments, as shown in FIG. 3, the edge of the bottom plate 1 is provided with a position-limiting leg 11 for limiting the position of the cell culture plate 2, the position-limiting leg 11 is an L-shaped structure perpendicular to the bottom plate 1 and is disposed at four edges of the bottom plate 1, and the four position-limiting legs 11 define a space for placing the cell culture plate 2 on the bottom plate 1. in other embodiments, for example, when the bottom plate 1 and the cell culture plate 2 are circular or elliptical plates, an annular groove may be disposed on the bottom plate 1 for placing and limiting the cell culture plate 2.
In some embodiments, to prevent the cell culture plate 2 from being damaged by impact, a pad 6 may be provided between the cell culture plate 2 and the base plate 1 for shock resistance, the pad 6 preferably being a resilient pad.
In some embodiments, the cell culture plate 2 is a standard culture plate and the cell culture wells 201 are evenly distributed on the cell culture plate 2. The cell culture plate 2 is preferably an ultra-low adsorption culture plate, and 3D organoids are prevented from adhering to the inner wall of the cell culture well 201. The number of cell culture wells 201 on the cell culture plate 2 is determined according to the number of 3D organoids to be cultured, and the present invention is not particularly limited, for example, the cell culture plate 2 may be a 296-well or 384-well ultra-low adsorption plate.
Both the base plate 1 and the cover plate 3 are made of a biocompatible material, for example, optically transparent grade pure polystyrene.
The embodiment of the utility model provides a 3D organoid's conveyer's assembly process as follows:
prior to the transport of 3D organoids, the inoculation of 3D organoids was performed: the prepared 3D balls are inoculated into the cell culture holes 201 of the cell culture plate 2, and the culture medium is added to wait for the 3D balls to settle to the bottom of the cell culture holes 201.
After the 3D ball is settled to the bottom of the cell culture plate 2, aligning the integrated air permeable piece 4 with the cell culture plate 2 and covering the integrated air permeable piece 2 above the cell culture plate 2, covering the cover plate 3 on the air permeable piece 4 and butting and embedding the integrated air permeable piece and the cell culture plate 2, then placing the cover plate 3, the air permeable piece 4 and the cell culture plate 2 which are butted and combined into a whole on the bottom plate 1, and limiting the placement position of the cell culture plate 3 through the limiting feet 1; finally, the buckle 5 is arranged at the four corners of the cover plate 3, the ventilating piece 4, the cell culture plate 2 and the bottom plate 1 which are sequentially overlapped to be fixed, and the assembly can be completed.
The embodiment of the utility model provides a culture system of 3D organoid still is provided, including foretell 3D organoid's conveyer. The transportation device can transport the 3D organs integrally in the 3D organ culture process, shorten the culture period and improve the culture efficiency; meanwhile, adhesion among the 3D organoids cannot occur, and the culture effect of the 3D organoids can be improved.
The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the protection scope of the present invention is defined by the claims. Various modifications and equivalents of the invention can be made by those skilled in the art within the spirit and scope of the invention, and such modifications and equivalents should also be considered as falling within the scope of the invention.
Claims (10)
1. A device for transporting a 3D organoid, comprising:
a base plate;
a cell culture plate provided on the bottom plate, the cell culture plate having a plurality of cell culture wells;
the cover plate is arranged at the top of the cell culture plate and is detachably connected with the cell culture plate, a hollow hole corresponding to the cell culture hole is formed in the cover plate, and a ventilating piece used for separating the cell culture hole from the outside is arranged between the hollow hole and the cell culture hole;
and a fixing mechanism for fixing the base plate, the cell culture plate and the cover plate integrally.
2. The 3D organoid transport device of claim 1, wherein the cell culture plate comprises a first plate and a first side plate bent vertically upward along an edge of the first plate; the cover plate comprises a second plate body and a second side plate which is vertically bent downwards along the edge of the second plate body, and the first side plate and the second side plate can be in butt joint and embedded.
3. The device as claimed in claim 1, wherein the gas permeable member is a one-piece structure and is disposed between the cell culture plate and the cover plate, and the gas permeable member is matched with the cell culture plate in size.
4. The device of claim 1, wherein the gas permeable member is a gas permeable medical silica gel layer.
5. The 3D organoid transporter according to claim 1, wherein the securing mechanism comprises a clasp.
6. The device for transporting a 3D organoid according to claim 1, wherein the edge of the bottom plate is provided with a limiting foot for limiting the cell culture plate.
7. The 3D organoid transport device of claim 1, wherein a backing plate is further disposed between the cell culture plate and the base plate.
8. The 3D organoid transporter according to claim 1, wherein the cell culture wells are evenly spaced on the cell culture plate.
9. The 3D organoid transporter according to claim 1, wherein the cell culture plate is a standard cell culture plate.
10. A culture system for a 3D organoid, comprising a delivery device for a 3D organoid according to any of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921838029.1U CN211170710U (en) | 2019-10-29 | 2019-10-29 | Conveying device and culture system for 3D (three-dimensional) organoid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921838029.1U CN211170710U (en) | 2019-10-29 | 2019-10-29 | Conveying device and culture system for 3D (three-dimensional) organoid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211170710U true CN211170710U (en) | 2020-08-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921838029.1U Active CN211170710U (en) | 2019-10-29 | 2019-10-29 | Conveying device and culture system for 3D (three-dimensional) organoid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211170710U (en) |
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2019
- 2019-10-29 CN CN201921838029.1U patent/CN211170710U/en active Active
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