CN210993797U - A microfiltration membrane module filter bacteria and antifouling performance tester - Google Patents

Abstract

The utility model provides a micro-filtration membrane component filter bacterium and anti-fouling performance tester, belonging to the testing technical field, comprising a filter membrane filter element filtering system and a microorganism detection system, wherein the filter membrane filter element filtering system comprises a water inlet system, a filtering system, a back washing system, a pressure testing system and a flux testing system; the microorganism detection system comprises a bacteria detector and a cell counter, wherein the bacteria detector and the cell counter are used for filtering and sterilizing in a cross-flow filtering mode, the transmembrane pressure difference is monitored in real time after filtering and sterilizing, whether a membrane assembly is polluted by microorganisms or not is evaluated, and backwashing operation is carried out.

Description

A microfiltration membrane module filter bacteria and antifouling performance tester

technical field

The technology belongs to the technical field of testing, and in particular relates to a microfiltration membrane component filter bacteria and antifouling performance tester.

Background technique

The microfiltration membrane is a porous and uniform surface film. The microfiltration membrane uses the static pressure difference as the driving force to separate the substances through the sieving effect of the microfiltration membrane; the microfiltration membrane separation technology can remove the gas phase or liquid phase substances. Colloids, bacteria and solids, thus enabling decontamination of mixtures. The microfiltration membrane can intercept all microorganisms or impurities of the size of microorganisms to ensure that no microorganisms pass through the membrane material and enter the downstream processing process; at the same time, the intercepted microorganisms or impurities and other pollutants can be removed by cross-flow without adhesion. On the surface of the microfiltration membrane, repeated use and extended life are achieved. However, the current microfiltration membrane filtration equipment on the market cannot achieve sufficient filtration and sterilization treatment and real-time detection of the existence of microorganisms in the water. The traditional method to detect the existence of bacteria in the water sample requires culturing the terminal water sample in the culture medium to observe whether the existence of bacteria exists. Colony growth is time-consuming and labor-intensive. In addition, there are no relevant equipment and test methods for the test of the anti-fouling performance of microfiltration membranes.

Chinese Patent Publication No. CN201464116U discloses a flat-plate filter element vacuum detector, although it can effectively detect the tightness of the flat-plate filter element membrane and the uniformity of the filter performance of multiple flat-plate filter elements, but Simply testing these parameters cannot guarantee the quality of the flat filter membrane element, mainly because the main factors affecting the quality of the flat filter membrane element are the water flux and anti-fouling of the filter membrane.

Chinese Patent Publication No. CN201310589128.1, discloses a detection device for flat filter membrane elements, the utility model uses a simulated fluid box for accommodating the flat filter membrane elements: the flat filter membrane element and the simulated fluid can be circulated at a fixed pressure Circulation device of fluid between tanks: a metering piece for detecting the flow rate of the fluid circulated by the circulation device. The detection device for flat filter membrane elements according to the present invention can effectively detect the water flux and anti-pollution property of the flat filter membrane elements, thereby ensuring the quality of the flat filter membrane elements, although the utility model can effectively detect the flat plate filter elements The water flux and anti-fouling performance of the filtration membrane element, but its disadvantage is that it cannot detect microorganisms in water instantly.

Therefore, there is an urgent need to develop a testing device that can simultaneously evaluate the performance of microfiltration membranes in removing bacteria and being able to be washed and reused.

Utility model content

In order to solve the above-mentioned technical problems, the utility model provides a microfiltration membrane module filter bacteria and antifouling performance tester, which is filtered and sterilized by cross-flow filtration, and uses real-time monitoring of the transmembrane pressure difference to evaluate whether the membrane module is damaged or not. Microbial contamination is backwashed, and the bacteria detector and cell counter are connected to the filtered water sample, which can instantly detect whether the water sample filtered through the membrane module contains microorganisms.

A microfiltration membrane component filtration bacteria and antifouling performance tester, including a membrane filter element filtration system and a microorganism detection system, the filter membrane filter element filtration system includes a water inlet system, a filtration system, a backwash system, a pressure test system and a flux Test system; the microorganism detection system includes a bacteria detector and a cell counter; after the water to be filtered enters the filtration system through the pipeline through the water inlet system, the filter element is washed by cross-flow filtration for filtration, and after the filtration is completed, the purified water enters the backwash The system is backwashed, and the sewage is returned to the influent system for re-filtration.

Further, the water inlet system includes a water inlet tank, a water pump, and a water inlet valve arranged at the front end of the water pump. The water inlet tank and the water pump are connected by pipelines, and the water inlet flow rate is controlled by adjusting the water inlet valve.

Further, the filtration system includes an immersion tank and a membrane module arranged in the immersion tank, and the inner cavity of the membrane module is located in the membrane module. The force perpendicular to the surface of the membrane module makes the water molecules filter through the membrane module, and the force parallel to the surface of the membrane module takes away the particles on the surface of the membrane module and reduces the pollution of the membrane module.

Further, the membrane module contains a filter membrane or a filter element, wherein the filter membrane is a microfiltration membrane with a pore size of 0.10 μm-0.22 μm.

Because the filtration membrane is a microfiltration membrane and an ultrafiltration membrane, the pore size is small, and under the drive of pressure, it can retain suspended solids, bacteria, some viruses and large-sized colloids.

Further, the pressure test system is composed of a nitrogen cylinder with a gas source, a pressure reducing valve and a pressure tank arranged at the gas cylinder mouth of the nitrogen cylinder, and the gas valve in the pressure tank is adjusted to adjust the pressure and read the pressure.

Further, the testing steps of the flux testing system are as follows: a constant pressure is provided by a pressure tester, after the water sample passes through the inner cavity of the membrane module in the filtration system under the pressure drive, and after the filtration of the membrane module, the filter membrane is recorded at this time. At the same time, through the water outlet valve and water tank device in the backwash system, the flux of the effluent water is recorded, and by comparing the changes of the membrane flux before and after filtration, the membrane fouling situation is analyzed, and the membrane module needs to be backwashed. The pressure tester provides a constant pressure range of 0.5 bar-1 bar.

When the transmembrane pressure difference is large, it is easy to cause the swaying force of the water flow to increase, which makes it easier for small molecular organics to form membrane pores and block the subsequent organic substances from passing through the membrane pores, thereby easily causing membrane fouling. The filtration performance of the filter element in the pool, the range of the transmembrane pressure difference is 0.5bar-1bar.

Further, the indication condition of the backwashing operation is: when the transmembrane pressure difference of the water sample passing through the filtration system is greater than 1 bar, the backwashing operation is performed.

Further, the process of the backwash operation is to use the filtered water in the water tank, open the backwash pump and the backwash valve in the backwash system, and backwash the membrane module with the filtered water in the water tank through the water pipe.

Further, the backwashing system adopts countercurrent backwashing and relaxation method for backwashing, wherein the countercurrent backwashing time is 20-30s, the relaxation time is 10-15min, and the intensity of the backwashing flux is 1.5-2% of the filtration flux intensity. times.

Further, the microorganism detection system includes a bacteria detector and a cell counter, and the bacteria detector and the cell counter are respectively connected with the filtered water sample, and can instantly detect whether the water sample filtered through the membrane module contains microorganisms.

Wherein, the fluorescence rapid detection method adopted by the cell counter (11) in the microorganism detection system, the fluorescence rapid detection method adopts the ATP luminescence principle, and the ATP luminescence principle is: adenosine triphosphate is abbreviated as ATP , is the energy carrier of all life activities. ATP in live bacteria will be maintained within a certain range. When bacteria die, ATP will be decomposed by intracellular enzymes in a short time, which will not affect the measurement of living microorganisms. ATP content can indirectly reflect the number of viable bacteria. The ATP bioluminescence method is that luciferase catalyzes the reaction of luciferin and ATP under the condition of magnesium ions to form a complex of luciferin-AMP, which is oxidized by oxygen molecules, causing luciferin to generate electrical excitation and emit light, releasing carbon dioxide and water, The luminescence intensity is linearly related to the ATP concentration to detect the number of viable bacteria.

The working principle of the utility model is as follows: open the water inlet valve, so that the water to be filtered in the water inlet tank enters the inner cavity of the membrane module through the pipeline through the water pump, and the water to be filtered entering the inner cavity of the membrane module is cross-flowed in the membrane module Filtration, two forces in the vertical direction are formed in cross-flow filtration. The force perpendicular to the surface of the membrane module enables water molecules to filter through the membrane module, and the force parallel to the surface of the membrane module takes away the particles on the surface of the membrane module and reduces the number of membrane modules. The clean water after cross-flow filtration enters the immersion tank and flows into the backwash system through the pipeline. By adjusting the water outlet valve and suction pump in the backwash system, the filtered clean water enters the water tank, and the backwash pump adjusts the backwash system. The flush valve makes the clean water flow into the immersion tank and flows through the inner cavity of the membrane module for backwashing. The sewage after cross-flow filtration passes through the inner cavity of the filter membrane module through the regulating return valve and returns to the water inlet tank through the pipeline for re-filtration.

beneficial effect

(1) The membrane assembly of the present utility model contains a microfiltration membrane. Because the filter membrane is a microfiltration membrane with a smaller pore size, under the drive of pressure, it can retain suspended solids, bacteria, some viruses and large-sized colloids, and achieve better filter effect.

(2) The utility model adopts the constant pressure mode filter device in the filtration test system, and under the constant pressure drive, by comparing the change of membrane flux, the pollution of the membrane module is analyzed, and the purpose of monitoring the pollution of the membrane module in real time is achieved.

(3) In the present utility model, the backwashing operation is controlled by monitoring the size of the transmembrane pressure difference in real time. Compared with the timed backwashing operation, the backwashing operation is controlled by monitoring the size of the transmembrane pressure difference. It can reduce the damage to the filter membrane and the filter element caused by the frequency of high-intensity backwashing, prolong the service life of the filter mesh membrane and the filter element, reduce the use of electric energy and water, save energy, and avoid untimely cleaning due to regular backwashing operation after membrane pollution. , resulting in blockage of the membrane pores, which in turn affects the filtration performance of the filtration membrane pool.

(4) The utility model adopts the method of cross-flow filtration, and two forces in the vertical direction are formed in the cross-flow filtration, the force perpendicular to the surface of the membrane module makes the water molecules filter through the membrane module, and the force parallel to the surface of the membrane module, Take away the particles on the surface of the membrane module to reduce the pollution of the membrane module.

(5) The ATP bioluminescence method adopted in the cell counter of the present invention has high sensitivity, fast detection speed and high accuracy.

Description of drawings

Fig. 1 is the schematic diagram of the utility model microfiltration membrane assembly filtration bacteria and antifouling performance tester device;

In the figure: 1. Nitrogen bottle; 2. Pressure reducing valve; 3. Pressure tank; 4. Immersion tank; 5. Membrane module; 6. Water outlet valve; 7. Backflushing valve; 8. Backflushing pump; 9. Water tank; 10. Suction pump; 11. Cell counter; 12. Bacteria detector; 13. Water inlet valve; 14. Water pump; 15. Inner cavity of membrane module; 16. Water inlet tank; 17. Return valve.

Detailed ways

The technical solutions of the various embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; Embodiments, all other embodiments obtained by those of ordinary skill in the art without creative work, all belong to the scope of protection of the present invention.

Example 1

A microfiltration membrane component filtration bacteria and antifouling performance tester, including a membrane filter element filtration system and a microorganism detection system, the filter membrane filter element filtration system includes a water inlet system, a filtration system, a backwash system, a pressure test system and a flux Test system; the microorganism detection system includes a bacteria detector 12 and a cell counter 11; after the water to be filtered enters the filtration system through the pipeline through the water inlet system, the filter component is washed by cross-flow filtration for filtration, and after the filtration is completed, the purified water enters The backwashing system is used for backwashing, and the sewage is returned to the water inlet system for re-filtration.

Further, the water inlet system includes a water inlet tank 16 , a water pump 14 and a water inlet valve 13 arranged at the front end of the water pump 14 .

Further, the filtration system includes an immersion tank 4 and a membrane module 5 arranged in the immersion tank 4. The inner cavity 15 of the membrane module is located in the membrane module 5. When the water to be filtered enters the inner cavity 15 of the membrane module, it is filtered by cross-flow. , forming two forces in the vertical direction, the force perpendicular to the surface of the membrane module 5 makes water molecules filter through the membrane module 5, and the force parallel to the surface of the membrane module 5 takes away the particles on the surface of the membrane module 5, reducing the membrane module 5. pollution.

Further, the membrane module 5 contains a filter membrane or a filter element, wherein the filter membrane is a microfiltration membrane with a pore size of 0.10 μm-0.22 μm.

Further, the pressure test system is composed of a nitrogen cylinder with a gas source, a pressure reducing valve and a pressure tank arranged at the gas cylinder mouth of the nitrogen cylinder, and the gas valve in the pressure tank is adjusted to adjust the pressure and read the pressure.

Further, the testing steps of the flux testing system are as follows: a constant pressure is provided by a pressure tester, and after the water sample passes through the inner cavity 15 of the membrane module in the filtration system under the pressure drive, after the filtration of the membrane module 5, the filtration is recorded at this time. The water flux of the membrane is recorded through the water outlet valve 6 and the water tank 9 device in the backwash system, and the flux of the effluent water is recorded. Backwash operation; the pressure tester provides a constant pressure range of 0.5bar-1bar.

Further, the indication condition of the backwashing operation is: when the transmembrane pressure difference of the water sample passing through the filtration system is greater than 1 bar, the backwashing operation is performed.

Further, the process of the backwash operation is to use the filtered water in the water tank 9, open the backwash pump 8 and the backwash valve 7 in the backwash system, and backwash the membrane module 5 with the filtered water in the water tank 9 through the water pipe. .

Further, the backwashing system adopts countercurrent backwashing and relaxation method for backwashing, wherein the countercurrent backwashing time is 20-30s, the relaxation time is 10-15min, and the intensity of the backwashing flux is 1.5-2% of the filtration flux intensity. times.

Further, the microorganism detection system includes a bacteria detector 12 and a cell counter 11. The bacteria detector 12 and the cell counter 11 are respectively connected to the filtered water sample, and can instantly detect whether the water sample filtered through the membrane module contains microorganism.

Example 2-3

Embodiment 2-3 provides a microfiltration membrane assembly filter bacteria and antifouling performance tester, compared with embodiment 1, the difference is that, except for the above differences, changing the pore size of the filter membrane, other operations are the same, The specific parameters are shown in Table 1. By preparing the bacterial solution, the performance analysis of the microfiltration membrane module filter bacteria and antifouling performance tester is carried out, and the preparation steps of the bacterial solution are as follows:

(1) Pour the recovery solution into a freezing tube containing freeze-dried Staphylococcus aureus strains, and shake gently to dissolve the freeze-dried strains into suspension. Burn the inoculation ring with an alcohol lamp until it turns red, and after cooling, dip the bacterial solution and gently scratch it on the slant medium, and incubate it in a constant temperature incubator at a constant temperature of 37 °C for 24 hours. After taking it out, use an inoculation loop to take a complete colony, put it into a conical flask containing 100 ml of sterile liquid medium, and shake it for 24 hours at 37° C. and 200 r/min to obtain Staphylococcus aureus bacterial liquid. The concentration of the suspension was determined by UV light to ensure that the viable bacterial count reached 10 ⁹ CFU/ml.

(2) Take 1 ml of ^{10 9} CFU of bacterial culture solution, dilute it into 1000 ml of sterile physiological saline, stir and mix evenly to prepare a bacterial suspension of 10 ⁶ CFU.

The prepared bacterial suspension was tested as the water source to be filtered.

Table 1:

It can be seen from Examples 1-3 that the pore size of the filter membrane is in the range of 0.10 μm to 0.22 μm. When the simulated filtered water source is tested for performance, the number of Staphylococcus aureus colonies is 0, indicating that the pore size of the filter membrane is between It has better filtering effect in the range of 0.10μm-0.22μm.

Examples 4-6

Embodiments 4-6 provide a microfiltration membrane module filtration bacteria and antifouling performance tester. Compared with embodiment 1, the difference is that the pressure in the pressure tester is changed. Except for the above differences, other operations are the same , which will not be repeated here, and the specific parameters are shown in Table 2 below. By preparing the bacterial solution, the performance analysis of the microfiltration membrane component filtration and antifouling performance tester is carried out, wherein the preparation steps of the bacterial solution are:

(1) Pour the recovery solution into a freezing tube containing freeze-dried Staphylococcus aureus strains, and shake gently to dissolve the freeze-dried strains into suspension. Burn the inoculation ring with an alcohol lamp until it turns red, and after cooling, dip the bacterial solution and gently scratch it on the slant medium, and incubate it in a constant temperature incubator at a constant temperature of 37 °C for 24 hours. After taking it out, use an inoculation loop to take a complete colony, put it into a conical flask containing 100 ml of sterile liquid medium, and shake it for 24 hours at 37° C. and 200 r/min to obtain Staphylococcus aureus bacterial liquid. The concentration of the suspension was determined by UV light to ensure that the viable bacterial count reached 10 ⁹ CFU/ml.

(2) Take 1 ml of ^{10 9} CFU of bacterial culture solution, dilute it into 1000 ml of sterile physiological saline, stir and mix evenly to prepare a bacterial suspension of 10 ⁶ CFU.

The prepared bacterial suspension was tested as the water source to be filtered.

Table 2:

It can be seen from Examples 4-6 that when the pressure range in the pressure tester is 0.5-1 bar, when the simulated filtered water source is tested for performance, the number of Staphylococcus aureus colonies is 0, indicating that the pressure tester provides a constant pressure of 0.5 At -1bar, the filtering performance of the filter element is better, it is not easy to block the filter element, and the filtering effect is better.

Examples 7-15

Embodiments 7-15 provide a microfiltration membrane module filtration bacteria and antifouling performance tester. Compared with Embodiment 1, the difference is that the countercurrent backwashing time, relaxation time, and backwashing flux strength are changed, except Except for the above differences, other operations are the same, which will not be repeated here. The specific parameters are shown in Table 3 below. By preparing the bacterial solution, the performance analysis of the microfiltration membrane component filtration and antifouling performance tester is carried out, wherein the preparation steps of the bacterial solution are:

(1) Pour the recovery solution into a freezing tube containing freeze-dried Escherichia coli strains, and shake gently to dissolve the freeze-dried strains into suspension. Burn the inoculation ring with an alcohol lamp until it turns red, and after cooling, dip the bacterial solution and gently scratch it on the slant medium, and incubate it in a constant temperature incubator at a constant temperature of 37 °C for 24 hours. After taking it out, use an inoculation loop to take a complete colony, put it into a conical flask containing 100 ml of sterile liquid medium, and shake it for 24 hours at 37° C. and 200 r/min to obtain Staphylococcus aureus bacterial liquid. The concentration of the suspension was determined by UV light to ensure that the viable bacterial count reached 10 ⁹ CFU/ml.

(2) Take 1 ml of ^{10 9} CFU of bacterial culture solution, dilute it into 1000 ml of sterile physiological saline, stir and mix evenly to prepare a bacterial suspension of 10 ⁶ CFU.

The prepared bacterial suspension was tested as the water source to be filtered.

table 3:

It can be seen from Examples 7-15 that when the countercurrent backwash time is 20-30s, the relaxation time is 10-15min, and the backwash flux intensity is 1.5-2 times the filtration flux intensity, the simulated filtered water source is During the performance test, the number of E. coli colonies was 0, indicating that under this backwashing condition, it was helpful to reduce the number of backwashing of the membrane module, reduce the loss of the membrane module, and improve the filtration performance of the membrane module.

The above description is only a preferred embodiment of the present invention, but the protection scope of the present invention is not limited to this. Equivalent replacement or modification of the new technical solution and its utility model concept shall be included within the protection scope of the present utility model.

Claims (3)

1. The utility model provides a micro-filtration membrane subassembly strains fungus anti-soil capability test appearance, includes filter membrane filter core filtration system and microorganism detecting system, its characterized in that: the filter membrane filter element filtering system comprises a water inlet system, a filtering system, a back washing system, a pressure testing system and a flux testing system;

the microorganism detection system comprises a bacteria detector (12) and a cell counter (11); the water inlet system comprises a water inlet tank (16), a water pump (14) and a water inlet valve (13) arranged at the front end of the water pump (14), the water inlet tank (16) is connected with the water pump (14) through a pipeline, and the flow of water inflow is controlled by adjusting the water inlet valve (13); the filtering system comprises an immersion tank (4) and a membrane module (5) arranged in the immersion tank (4), and an inner cavity (15) of the membrane module is positioned in the membrane module (5); the microorganism detection system comprises a bacterium detector (12) and a cell counter (11) which are arranged on two sides of the inner cavity (15) of the membrane component, wherein the bacterium detector (12) and the cell counter (11) are respectively connected with a filtered water sample, and can immediately detect whether the water sample filtered by the membrane component (5) contains microorganisms.

2. The microfiltration membrane assembly bacterial filtration and anti-fouling performance tester according to claim 1, wherein: the membrane component (5) contains a filtering membrane or a filter element, wherein the filtering membrane is a microfiltration membrane with the pore diameter of 0.10-0.22 μm.

3. The microfiltration membrane assembly bacterial filtration and anti-fouling performance tester according to claim 1, wherein: the pressure testing system is composed of a nitrogen cylinder (1) with an air source, a pressure reducing valve (2) arranged at the air bottle opening of the nitrogen cylinder (1) and a pressure tank (3), and is used for adjusting the pressure of an air valve in the pressure tank (3) and reading the pressure.

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