CN210506331U - A high-concentration semi-open continuous culture device for Chlorella - Google Patents
A high-concentration semi-open continuous culture device for Chlorella Download PDFInfo
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- CN210506331U CN210506331U CN201921013400.0U CN201921013400U CN210506331U CN 210506331 U CN210506331 U CN 210506331U CN 201921013400 U CN201921013400 U CN 201921013400U CN 210506331 U CN210506331 U CN 210506331U
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- chlorella
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- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims abstract description 31
- 239000000463 material Substances 0.000 claims abstract description 28
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims abstract description 9
- 229910000831 Steel Inorganic materials 0.000 claims abstract description 6
- 239000010959 steel Substances 0.000 claims abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000009344 polyculture Methods 0.000 abstract description 2
- 239000010865 sewage Substances 0.000 abstract description 2
- 238000009423 ventilation Methods 0.000 abstract 1
- 241000195493 Cryptophyta Species 0.000 description 8
- 239000007789 gas Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 230000001651 autotrophic effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000143060 Americamysis bahia Species 0.000 description 1
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 1
- 229940022405 astaxanthin Drugs 0.000 description 1
- 235000013793 astaxanthin Nutrition 0.000 description 1
- 239000001168 astaxanthin Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a chlorella high concentration semi-open continuous culture device. Comprises a cylinder body, a cylinder frame and an inflator 3 part. The cylinder body is made of 12mm thick glass, the specific size is 2000 multiplied by 700 multiplied by 800mm, the cover is made of 4 plates (the size is 500 multiplied by 700 mm), 2 acrylic plates and 2 breathable gauze plates, 2 sewage outlets are installed at the bottom of the glass cylinder, 3 material outlets are installed at the 80mm high position in front of the cylinder body, and a nano ventilation pipe is additionally arranged at the bottom of the glass cylinder. The cylinder frame is made of galvanized square steel with the size of 50mm multiplied by 50mm, the size of the cylinder frame is 2000 mm multiplied by 700mm multiplied by 500mm, and a transverse lining is added every 50 mm. One end of the air duct of the air charging device is connected with the air pump, and the other end is connected with the nanometer air duct in the cylinder. The utility model discloses the device can carry out polyculture continuous culture and autotrophy continuous culture to the chlorella, easy operation, with low costs, and raiser and manufacturing enterprise all can utilize the device to produce.
Description
Technical Field
The utility model relates to a little algae field of cultivateing especially relates to a high concentration semi-open continuous culture device of chlorella.
Background
Chlorella is an important microalgae resource and widely distributed in various water bodies such as lakes and oceans. The chlorella contains 20 amino acids required by animals due to high protein content, and is also rich in unsaturated fatty acid, carotenoid, astaxanthin, multiple vitamins and the like, so that the chlorella can be directly used as high-quality bait for fish, shrimps and shellfish in aquaculture to promote the growth of aquaculture organisms and the activity of digestive enzymes, and can reduce the content of nitrite, ammonia nitrogen, nitrate and phosphate in aquaculture water, thereby effectively purifying the aquaculture water.
The chlorella culture apparatus reported so far has 3 types of closed, semi-closed and open apparatuses. Although the yield of chlorella is high, the closed device has high investment cost and is difficult to install and maintain, and the closed device is suitable for health-care product enterprises with high added values; the semi-closed device in CN 207210411U has no material outlet, can not realize continuous culture, and the sealing of the bucket opening is not beneficial to the overflow of the generated oxygen, although the device is simple; the open culture device has low cost, large occupied area, easy pollution and low final algae density. The material inlet and outlet of the device in CN 205774510U are arranged at the top, so that the shunt is not realized, the operation is inconvenient, and the continuous culture cannot be realized.
Disclosure of Invention
The utility model aims to provide a simple, low-cost and easy-operating device for the high-concentration continuous culture of chlorella.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a high-concentration semi-open continuous culture device for chlorella is characterized by comprising a cylinder body, a cylinder frame and an air charging device; an acrylic plate cover 1 and a movable gauze plate 2 are arranged above the cylinder body 3, and the movable gauze plate can be opened and is a material inlet; two blowoff valves 4 are arranged on the left side and the right side of the bottom of the cylinder body, three material outlets 6 are arranged on the side surface of the cylinder body, the cylinder frame 7 is galvanized square steel with the size of 50mm multiplied by 50mm, the size of the cylinder frame is 2000 mm multiplied by 700mm multiplied by 500mm, and a transverse lining is added for supporting the cylinder body 3 every 50 mm; the inflator includes: a nano air pipe 5, an air duct 8 and an air pump 9; one end of the air duct 8 is connected with the air pump 9, and the other end is connected with the nanometer air duct 5 at the bottom of the cylinder body.
Semi-open continuous culture apparatus of chlorella high concentration, wherein 3 tops of cylinder body are equipped with two acrylic plate apron, two ventilative activity gauze boards. The cylinder body is a cuboid glass cylinder with the thickness of 12 mm. The dimensions are 2000X 700X 800 mm. The movable gauze plate is two layers of gauze.
The utility model discloses more detailed description is as follows:
a high-concentration semi-open continuous culture device for Chlorella comprises 3 parts, a cylinder body, a cylinder frame and an air charging device. A material inlet and an air duct inlet are arranged above the cylinder body, a material outlet is arranged on the side surface, and a drain outlet and a nano inflation tube are arranged at the bottom; the cylinder frame is galvanized square steel; the aerating device comprises an air pump, an air guide pipe and a nano aerating pipe, wherein the nano aerating pipe is arranged at the bottom of the tank, so that the aerating can be carried out, the uniform culture medium can be mixed, and the chlorella can be prevented from sinking to the bottom in the growth process. The utility model discloses the device can carry out polyculture continuous culture and autotrophy continuous culture to the chlorella, easy operation, with low costs, and raiser and manufacturing enterprise all can utilize the device to produce.
Preferably, the culture container is a glass jar, so that the light transmittance can be ensured, and harmful substances generated by the reaction of the plastic and the culture medium chemical reagent can be prevented.
Preferably, a bottom nanometer aerating device is adopted, bubbles are large in contact surface of the culture medium, the floating flow rate is low, the contact time is long, the mass transfer efficiency of gas is high, algae cells can be prevented from sinking, and the algae liquid is uniform.
Further, the material import is the gauze board of activity, can open during the feeding, and the material is covered after adding, can prevent that dust, mosquito etc. from getting into the pollution, has gas permeability and light transmissivity, conveniently washs the cylinder body in addition.
Furthermore, the material outlet is arranged at the position 80mm away from the cylinder body on the side surface of the cylinder body, the chlorella can be conveniently taken and used, and 1/10 remained substrates can be reserved as the chlorella seeds to realize continuous culture.
Furthermore, the sewage draining port is arranged at the bottom of the tank, so that waste water is drained when the tank is convenient to clean.
Furthermore, the glass jar is arranged on the jar frame and is provided with a material outlet, so that the cultured chlorella can be conveniently taken.
The utility model discloses a chlorella high concentration semi-open continuous culture device compares the enthusiasm effect that has with prior art and lies in:
(1) the chlorella culture device can be used for autotrophic culture and mixed culture of chlorella, the chlorella grows quickly and has high density, mixed culture can be completed in 3-5 days generally, and autotrophic culture can be completed in 7-10 days.
(2) Can prevent chlorella from sinking to the bottom and realize continuous culture.
(3) The operation is simple and convenient, the cost is low, the device can be used repeatedly, and the device is suitable for farmers to use in own courtyards and pond mouths.
Drawings
Fig. 1 is a schematic structural diagram of the present invention:
in the figure: 1-acrylic cover plate, 2-movable gauze plate, 3-cylinder body, 4-blowoff valve, 5-nano vent pipe, 6-material outlet, 7-cylinder frame, 8-air duct, 9-air pump.
Detailed Description
In order to make the advantages and technical solutions of the present invention more clearly understood, the present invention will be described in detail below with reference to the accompanying drawings, but the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example 1
A high-concentration semi-open continuous culture device for chlorella comprises a cylinder body, a cylinder frame and an air charging device; two acrylic plate cover plates 1 and two breathable movable gauze plates 2 are arranged above the cylinder body 3, and the movable gauze plates can be opened; the cylinder frame is a material inlet, two blowoff valves 4 are arranged on the left side and the right side of the bottom of the cylinder body, three material outlets 6 are arranged on the side surface of the cylinder body, the cylinder frame 7 is galvanized square steel with the size of 50mm multiplied by 50mm, the size of the cylinder frame is 2000 mm multiplied by 700mm multiplied by 500mm, and a transverse lining is added for supporting the cylinder body every 50 mm; the inflator includes: a nano vent pipe, a gas guide pipe and an air pump; one end of the air duct 8 is connected with the air pump 9, and the other end is connected with the nanometer air duct 5 at the bottom of the cylinder body.
Example 2
As shown in FIG. 1, a high concentration semi-open continuous culture apparatus for Chlorella comprises 3 parts, a cylinder frame and an air charging device. The cylinder body 3 is a glass cylinder with the thickness of 12mm, is firm and strong in light transmission, can prevent a plastic container from reacting with chemical reagents in culture medium raw materials and releasing harmful substances, and has the preferred size of 2000 multiplied by 700 multiplied by 800mm and the volume of about 1 ton. Two fixed acrylic cover plates 1 and two movable gauze plates 2 are arranged above the cylinder body, and the sizes of the two fixed acrylic cover plates and the two movable gauze plates are 500mm multiplied by 700 mm. The acrylic cover plate with strong light transmission is fixed on the cylinder body, a small hole is arranged on the left side of the acrylic cover plate and is used as an air duct inlet, the movable gauze cover plate can be used as a material inlet when being opened, and the movable gauze cover plate is breathable and light-transmitting when being covered and can block dust and insects. The side surface is 80mm high from the bottom, a material outlet is arranged at intervals of 500mm, three material outlets are arranged, a stainless steel water tap is preferably selected as the material outlet, so that the material inlet and the material outlet are separated, the taking is convenient, the algae liquid which cannot be discharged below the material outlet can be used as algae seeds, the inoculation amount is about 10%, and the continuous culture can be realized by adding a new culture medium and water. The left side and the right side of the bottom are respectively provided with a drain outlet, the diameter of the drain outlet is preferably 25mm, and the drain outlet is matched with an external thread and a water valve, so that waste liquid is discharged when the cleaning and the disinfection are convenient, and the drain outlet can be used as a material outlet when the cleaning and the disinfection are not used in winter, so that the material is completely discharged. Two rows of nano gas-filled tubes are arranged at the bottom, so that the mass transfer efficiency of gas can be improved, and the algae cells can be prevented from sinking. The cylinder frame 7 is galvanized square steel with the size of 50mm multiplied by 50mm, the size is 2000 mm multiplied by 700mm multiplied by 500mm, a transverse lining is added every 50mm for supporting the cylinder body, the materials are convenient to take and use, the cylinder frame can be fixed, and universal wheels can be installed at the bottom of the cylinder frame, so that the cylinder frame is convenient to move. The air duct 8 is a high temperature resistant silicone tube, one end of the air duct is connected with the air pump 9, and the other end of the air duct is connected with the nano inflation tube 5.
Example 3
The utility model discloses the device shows with conventional device contrast condition:
and (4) conclusion: the utility model discloses the advantage: 1. the glass container can prevent the conventional plastic container from reacting with the chemical raw materials of the culture medium to generate harmful substances. 2. The nano aeration can increase the mass transfer efficiency of the gas and prevent the algae cells from sinking. 3. The material inlet and outlet are separated, the material is fed from the upper part, and discharged from the lower part, so that the operation is convenient, and the continuous culture can be realized. 4. The substrate is provided with a drain valve, so that the waste cleaning liquid can be conveniently discharged.
Claims (5)
1. A high-concentration semi-open continuous culture device for chlorella is characterized by comprising a cylinder body, a cylinder frame and an air charging device; an acrylic plate cover plate (1) and a movable gauze plate (2) are arranged above the cylinder body (3), the movable gauze plate can be opened and is a material inlet, two drain valves (4) are arranged on the left side and the right side of the bottom of the cylinder body, and three material outlets (6) are arranged on the side surface of the cylinder body; the cylinder frame (7) is galvanized square steel with the thickness of 50mm multiplied by 50mm, the size of the cylinder frame is 2000 mm multiplied by 700mm multiplied by 500mm, and a transverse lining is added for every 50mm to support the cylinder body (3); the inflator includes: the air pump comprises a nanometer air pipe (5), an air guide pipe (8) and an air pump (9), wherein one end of the air guide pipe (8) is connected with the air pump (9), and the other end of the air guide pipe is connected with the nanometer air pipe (5) at the bottom of the cylinder body.
2. The chlorella high-concentration semi-open continuous culture apparatus according to claim 1, wherein: and 2 acrylic plate cover plates (1) and 2 breathable movable gauze plates (2) are arranged above the cylinder body (3).
3. The chlorella high-concentration semi-open continuous culture apparatus according to claim 1, wherein: the cylinder body (3) is a cuboid glass cylinder with the thickness of 12 mm.
4. The chlorella high-concentration semi-open continuous culture apparatus according to claim 1, wherein: the cylinder body (3) has the size of 2000 multiplied by 700 multiplied by 800 mm.
5. The chlorella high-concentration semi-open continuous culture apparatus according to claim 1, wherein: the movable gauze plate (2) is provided with two layers of gauze.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921013400.0U CN210506331U (en) | 2019-07-02 | 2019-07-02 | A high-concentration semi-open continuous culture device for Chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921013400.0U CN210506331U (en) | 2019-07-02 | 2019-07-02 | A high-concentration semi-open continuous culture device for Chlorella |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210506331U true CN210506331U (en) | 2020-05-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921013400.0U Active CN210506331U (en) | 2019-07-02 | 2019-07-02 | A high-concentration semi-open continuous culture device for Chlorella |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210506331U (en) |
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2019
- 2019-07-02 CN CN201921013400.0U patent/CN210506331U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210120 Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Patentee after: Tianjin Academy of Agricultural Sciences Address before: 300384 17 kilometers, Jin Jing Road, Xiqing District, Tianjin Patentee before: TIANJIN AGRICULTURAL BIO-TECH RESEARCH CENTER |