CN210340927U - Cell separator - Google Patents
Cell separator Download PDFInfo
- Publication number
- CN210340927U CN210340927U CN201920496155.7U CN201920496155U CN210340927U CN 210340927 U CN210340927 U CN 210340927U CN 201920496155 U CN201920496155 U CN 201920496155U CN 210340927 U CN210340927 U CN 210340927U
- Authority
- CN
- China
- Prior art keywords
- cell
- separator
- cells
- connecting portion
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000926 separation method Methods 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000002105 nanoparticle Substances 0.000 claims description 26
- 230000005684 electric field Effects 0.000 claims description 19
- 210000004027 cell Anatomy 0.000 description 95
- 229920000642 polymer Polymers 0.000 description 10
- 238000000034 method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 239000011324 bead Substances 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229920000767 polyaniline Polymers 0.000 description 3
- 101001078133 Homo sapiens Integrin alpha-2 Proteins 0.000 description 2
- 102100025305 Integrin alpha-2 Human genes 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- -1 carboxyl-amino Chemical group 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000004720 dielectrophoresis Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 229920002845 Poly(methacrylic acid) Polymers 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000003320 cell separation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229920001940 conductive polymer Polymers 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004375 physisorption Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Electrostatic Separation (AREA)
Abstract
The utility model discloses a cell separator, the separator includes the separator main part and rather than a plurality of separation passageways that are linked together, the separator main part is used for holding the cell and mixes the liquid, different cells in the cell mixes the liquid combine with different dielectric constant's separator specificity respectively, direct current or alternating voltage are applyed through the controller to the separator main part, make different cells get into different separation passageways and separate.
Description
Technical Field
The utility model relates to a cell separator belongs to biotechnology field.
Background
The current cell sorting process is that firstly, the biological specificity of nano magnetic beads is connected to cells, then a sorting column capable of enhancing the magnetic field distribution is placed in the magnetic field of a permanent magnet, the cells connected with the nano magnetic beads are adsorbed on the column when a cell solution passes through the separating column, then the unnecessary cells are washed off and separated, finally, the separating column is removed from the magnetic field, and the cells adsorbed on the separating column are re-dispersed by using a dispersion liquid.
Because the magnetic field intensity of a single permanent magnet is fixed and cannot be modulated, the magnetic field distribution is complex after the number of magnets is increased, the use is difficult, the production process of magnetic beads is complex, the cost is high, and the price is high, the cell sorting mode can only sort one cell at a time, the automation difficulty is high, the flux is low, and the cell sorting column is difficult to recycle.
Disclosure of Invention
An object of the utility model is to provide a cell separator to can divide fast and elect multiple cell, and this kind of separation mode can incessantly select separately a large amount of solutions, has improved the flux greatly.
In order to achieve the above purpose, the utility model provides a following technical scheme:
the utility model provides a cell separator, including separator main part and a plurality of separation passageways rather than being connected, the separator main part is used for holding the cell and mixes the liquid, the cell of difference in the cell mixes the liquid combines with the separator specificity of different dielectric constants respectively, the separator main part is applyed direct current or alternating voltage through the controller and is formed direct current or alternating electric field, makes different cells get into different separation passageways and separates at the decurrent in-process.
Further, the isolate is a nanoparticle or a biomolecule.
Further, the cell separator still includes first connecting portion, the one end of first connecting portion with the separator main part communicates mutually, the other end of first connecting portion with a plurality of separation channel are linked together, through the setting of first connecting portion, more are favorable to the separation of cell mixed liquid.
Furthermore, the width of one end of the first connecting part communicated with the plurality of separation channels is larger than that of the other end of the first connecting part communicated with the separator main body, so that the cell mixed liquid can be more fully separated, the liquid flow can be prolonged, and the cell mixed liquid can be more fully separated.
Further, the separation channel includes a second connection portion and a cell outflow portion, one end of the second connection portion communicates with the first connection portion, and the other end communicates with the cell outflow portion.
Further, the width of the end of the second connecting part communicated with the first connecting part is larger than that of the other end of the second connecting part communicated with the cell outflow part, and the separated cells can be further collected while the cells are fully separated by designing the separation channel into an inverted cone shape.
Furthermore, positive and negative electrodes are respectively loaded on two sides of the separator main body and are respectively connected with the controller through leads.
The beneficial effects of the utility model reside in that: the method specifically adsorbs nanoparticles or biomolecules with different dielectric constants onto cells, can generate different responses in an alternating current electric field or a direct current electric field due to different high-frequency electric field polarization effects or different positive and negative charge intensity differences of the biomolecules or the nanoparticles, specifically connects particles or molecules with greatly different electric field responses to the cells, then places the cells connected with the nanoparticles or the molecules in the alternating current electric field or the direct current electric field, and separates and purifies the cells by adjusting the electric field and the different responses of the nanoparticles or the molecules to the electric field by applying an electrophoresis or dielectrophoresis principle.
The synthesis modes of the nanoparticles with different polarities are various, and the nanoparticles with good biocompatibility can be used, so that the dependence on the nano magnetic beads in the traditional method is reduced, a complex cell sorting column does not need to be produced, and the cost is reduced on the whole. The cell sorting technology based on the electric field regulation does not enable cells to be physically adsorbed on the sorting column, and biomolecules can be used for connecting the cells to achieve the result of changing polarity or electric charge, so that the physical damage to the cells is reduced, great help is provided for the experiment using the cells later, the electric field regulation mode is simple and easy to operate, the sorting equipment can be recycled, and various cells can be rapidly sorted through molecules or nanoparticles with different polarities. The sorting mode can continuously sort a large amount of solution, and greatly improves the flux.
The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings.
Drawings
FIG. 1 is a schematic structural view of a cell separator according to the present invention;
wherein:
1. the cell separation device comprises a separator body, 2. a separation channel, 201. a second connecting part, 202. a cell outflow part, 3. a cell mixed solution, 4. a controller, 5. a lead, 6. an electrode and 7. a first connecting part.
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
As shown in FIG. 1, the cell separator of the present invention comprises a separator body 1 and a plurality of separation channels 2 connected thereto, wherein the separator body 1 is used for containing a cell mixture 3, different cells in the cell mixture 3 are specifically combined with separators with different dielectric constants, the separator body 1 applies DC or AC voltage through a controller 4 to form a DC or AC electric field, so that different cells enter different separation channels 2 to be separated in the down-flow process. In specific implementation, the separator can be nanoparticles or biomolecules, positive and negative electrodes 6 are respectively loaded on two sides of the separator body 1, the positive and negative electrodes 6 are respectively connected with a controller 4 through leads 5, and the number of the separation channels 2 is four.
In the above embodiment, the cell separator further includes the first connection portion 7, one end of the first connection portion 7 is communicated with the separator main body 1, and the other end of the first connection portion 7 is communicated with the plurality of separation channels 2, so that the cell mixture can be separated more easily by the arrangement of the first connection portion 7. In specific implementation, the width of the end of the first connecting part 7 communicated with the plurality of separation channels 2 is larger than the width of the other end of the first connecting part 7 communicated with the separator main body 2, so that the cell mixed liquid can be more fully separated, the liquid flow can be prolonged, and the cell mixed liquid can be more fully separated.
In the above-described embodiment, the separation channel 2 includes the second connection part 201 and the cell outflow part 202, and one end of the second connection part 201 communicates with the first connection part 7 and the other end communicates with the cell outflow part 202. In specific implementation, the width of the end of the second connection part 202, which is in communication with the first connection part 7, is greater than the width of the other end of the second connection part 7, which is in communication with the cell outflow part 202, and the separation channel 2 is designed to be an inverted cone, so that the separated cells can be further collected while the cells are sufficiently separated.
In the above embodiment, when the controller 4 applies the dc voltage, the separator-linked cells with high density of positive charges move farthest toward the negative electrode, the separator-linked cells with low density of positive charges move toward the negative electrode but are shorter than the moving distance of high density of positive charges, the separator-linked cells with high density of negative charges move farthest toward the positive electrode, the separator-linked cells with low density of negative charges move toward the positive electrode but are shorter than the moving distance of high density of negative charges, and the separator-linked cells with different densities of positive and negative charges move toward the positive and negative electrodes respectively by different distances in the direction perpendicular to the solution flow direction and finally flow into different separation channels 2. In specific implementation, when the controller 4 applies a dc voltage, the isolate is a nanoparticle or a biomolecule, and the dc voltage ranges from plus or minus 100V.
In the above embodiment, when the controller 4 applies the ac voltage, the cells connected to the separators with different dielectric constants or conductivities flow to the positive and negative electrodes for different distances under different dielectrophoretic forces of the separators in the alternating electric field, and the magnitude of the dielectrophoretic force can be changed by changing both the voltage and the frequency, so that the difference in the distances traveled by the different cells connected to the separators with different dielectric constants or conductivities within the same time can be changed by changing the voltage or the frequency, so that the different cells can accurately flow to the different separation channels 2 and be separated finally. In specific implementation, when the controller 4 applies an alternating voltage, the separation substance is nanoparticles, the voltage range is plus or minus 100V, and the frequency is 1Hz to 1 MHz.
Example 1
For a direct current electric field, the principle of electrophoresis can be applied. Firstly, synthesizing metal or polymer nanoparticles with different positive and negative charge strengths and high biocompatibility, in the embodiment, adopting a copolymer of polyethylene glycol and polymethacrylic acid or polyethyleneimine, and finally synthesizing four kinds of polymer nanoparticles with the diameter of 50 nanometers by adjusting the proportion of the two kinds of polymers, wherein the Zeta potentials are +10, +40, -10, -40mV respectively. The two polymer particles can be connected with specific antibodies such as CD4, CD8a, CD49b and Gr-1 through carboxyl-amino coupling reaction, then connected to the cell surface with the four receptors on the surface through nanoparticles connected with the specific antibodies, and finally the systematic surface charge amount after the coupling of the cells and the nanoparticles is controlled by controlling the concentration ratio to control the number of the nanoparticles coupled to each cell.
The cell mixed solution connected with the nano-particles is introduced into the cell separator body 1, and a direct current voltage of 20V is continuously applied in a direction perpendicular to the cell flowing direction, or a direct current voltage of plus or minus 10V is applied in a sine wave or other waveforms. The flowing cells move towards the negative pole direction due to different charges on the surfaces, the cells with positive charges move towards the positive pole direction, and the cells with negative charges move towards the positive pole direction. In this way, the cells flow into the independent flow channels 2 respectively when flowing to the bottom of the flow channel, and finally four kinds of cells are collected at the outlets of the independent flow channels 2 respectively.
Example 2
For alternating electric fields, the principle of dielectrophoresis can be applied. Firstly, metal or polymer nanoparticles with different dielectric constants and high biocompatibility are synthesized, in this embodiment, a copolymer of polyethylene glycol and polyaniline is adopted, and four kinds of polymer nanoparticles with a diameter of 50 nanometers are finally synthesized by adjusting the proportion of two kinds of polymers. The response speed of polymer nanoparticles in a high-frequency electric field can be greatly improved by the conductive polymer accumulated by polyaniline, particularly, when the relative frequency is low, the conductivity is dominant in the dielectric constant, and the polymer nanoparticles can show great difference by slightly adjusting the content of the polyaniline in the frequency electric field range of 0.1-10 MHz. The polymer particles can be connected with specific antibodies such as CD4, CD8a, CD49b and Gr-1 through carboxyl-amino coupling reaction, then connected to the cell surface with the four receptors on the surface through nanoparticles connected with the specific antibodies, and finally the systematic dielectric constant after the coupling of the cells and the nanoparticles is controlled by controlling the concentration ratio and controlling the number of the coupled nanoparticles of each cell.
The cell mixed solution connected with the nano-particles is introduced into the cell separator body 1, and an alternating voltage of 20V is continuously applied in an asymmetric electrode shape in a direction perpendicular to the cell flow direction, wherein the voltage frequency is 1MHz or the frequency is changed from 10kHz to 1MHz in a sine wave and other waveform modes, so that the cell separation distance is large. The flowing cells can move towards a region with higher or lower electric field line density in a non-uniform field due to different effective dielectric constants, and are separated more and more far particularly under the driving of sine wave and other waveform frequency changes, but the distance between the position of the cells in the flow channel, which is reversely changed in the cell moving direction, and the initial position is not too far and is relatively symmetrical due to the change of the frequency range, and through the mode, the cells respectively flow into the independent flow channels 2 when flowing to the bottom of the flow channel, and finally, four cells are respectively collected at the outlets of the independent flow channels 2.
In addition, through the rate of recovery of the cell that the above-mentioned embodiment 1 and 2 separated of experimental determination, the result shows, the utility model discloses a method of separating is carried out to the cell, compares the cell separation method among the prior art, and the rate of recovery of its cell has obvious improvement to because can separate a plurality of cells simultaneously, the separation flux is high, in addition, the utility model discloses a separation method does not make cell physisorption on sorting column, can use biomolecule to connect the cell moreover, reaches the result that changes polarity or electric charge, has reduced the physical destruction to the cell, has very big help to the experiment of later use cell.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (5)
1. A cell separator is characterized by comprising a separator main body, a plurality of separation channels and a first connecting part for connecting the separator main body and the separation channels, wherein the separator main body is used for containing cell mixed liquid, and a controller applies direct current or alternating current voltage to form a direct current or alternating current electric field so as to enable different cells to enter different separation channels for separation; the controller is electrically connected with a positive electrode and a negative electrode for supplying power.
2. The cell separator of claim 1, wherein the separator is a nanoparticle or a biomolecule.
3. The cell separator according to claim 2, wherein a width of one end of the first connecting portion communicating with the plurality of separation channels is larger than a width of the other end of the first connecting portion communicating with the separator body.
4. The cell separator according to claim 3, wherein the separation channel includes a second connecting portion and a cell outflow portion, the second connecting portion having one end communicating with the first connecting portion and the other end communicating with the cell outflow portion.
5. The cell separator according to claim 4, wherein the width of one end of the second connecting portion, which communicates with the first connecting portion, is larger than the width of the other end of the second connecting portion, which communicates with the cell outflow portion.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018103256249 | 2018-04-12 | ||
| CN201810325624 | 2018-04-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210340927U true CN210340927U (en) | 2020-04-17 |
Family
ID=68248529
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920496155.7U Active CN210340927U (en) | 2018-04-12 | 2019-04-12 | Cell separator |
| CN201910294971.4A Pending CN110373320A (en) | 2018-04-12 | 2019-04-12 | Cell separator and its separation method |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910294971.4A Pending CN110373320A (en) | 2018-04-12 | 2019-04-12 | Cell separator and its separation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN210340927U (en) |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7169275B2 (en) * | 2002-01-21 | 2007-01-30 | Tecan Trading Ag | Method for separating particles in free flow electrophoresis |
| US7964078B2 (en) * | 2005-11-07 | 2011-06-21 | The Regents Of The University Of California | Microfluidic device for cell and particle separation |
| TWI335936B (en) * | 2006-11-03 | 2011-01-11 | Raydium Semiconductor Corp | Method of arranging cells and electrode pattern applying thereto |
| JP5098324B2 (en) * | 2006-12-19 | 2012-12-12 | パナソニック株式会社 | Fuel cell separator and fuel cell |
| JP2009065967A (en) * | 2007-08-20 | 2009-04-02 | Gunma Univ | Cell separation device and cell separation method |
| KR101793128B1 (en) * | 2008-07-16 | 2017-11-02 | 칠드런'즈 메디컬 센터 코포레이션 | Organ mimic device with microchannels and methods of use and manufacturing thereof |
| CN102302898A (en) * | 2011-06-17 | 2012-01-04 | 西安交通大学 | Micro blood cell separation device and method for using same |
| CN102286423B (en) * | 2011-06-30 | 2014-05-28 | 西安交通大学 | Method for improving polyethylene glycol glial cell affinity |
| WO2013058742A1 (en) * | 2011-10-18 | 2013-04-25 | Empire Technology Development Llc | Closed-cycle continuous flow separators, systems and methods for the continuous isolation of target cells |
| CN102581298A (en) * | 2012-02-20 | 2012-07-18 | 山东大学 | Gold nanoparticle array with continuously-changed surface charge density |
-
2019
- 2019-04-12 CN CN201920496155.7U patent/CN210340927U/en active Active
- 2019-04-12 CN CN201910294971.4A patent/CN110373320A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN110373320A (en) | 2019-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sonker et al. | Separation phenomena in tailored micro-and nanofluidic environments | |
| Marquet et al. | Rectified motion of colloids in asymmetrically structured channels | |
| Markx et al. | Dielectrophoretic separation of cells: Continuous separation | |
| WO2006025982B1 (en) | Rapid flow fractionation of particles combining liquid and particulate dielectrophoresis | |
| WO2006058245A2 (en) | Dielectrophoretic particle sorter | |
| Lee et al. | Magnetic-nanoflocculant-assisted water–nonpolar solvent interface sieve for microalgae harvesting | |
| US20080296157A1 (en) | Method and Device for Handling Sedimenting Particles | |
| Lapizco-Encinas | Microscale electrokinetic assessments of proteins employing insulating structures | |
| KR20070082698A (en) | Particle Separation Device and Particle Separation Method | |
| CN113717846B (en) | A cell sorting chip, device and method based on dielectric deterministic displacement | |
| CN210340927U (en) | Cell separator | |
| SG190934A1 (en) | Method and apparatus for the separation of oil and water using hydrophobic and hydrophilic functional solid particles | |
| KR20090002980A (en) | Micro Particle Processing Equipment | |
| Zhang et al. | Continuous electrodeless dielectrophoretic separation in a circular channel | |
| US20040114458A1 (en) | Device for mixing fluids | |
| WO2025237235A1 (en) | Apparatus for performing direction control and separation on charged particles in electrolyte | |
| CN1995361A (en) | Method for promoting electroporation efficiency utilizing dielectrophoresis for assisting cell positioning | |
| KR20100045686A (en) | Devices for particle concentration and separation using electric current density differences in plate electrodes | |
| Arnold | Particle patterning using fluidics and electric fields | |
| US8329015B2 (en) | Dielectrophoretic process for retaining polarizable target-particles and device for performing that process | |
| Hakoda | Development of dielectrophoresis separator with an insulating porous membrane using DC‐Offset AC Electric Fields | |
| Giesler | Process Development of Electrode-Based Dielectrophoretic Separators | |
| US20150075987A1 (en) | Devices and methods for separating nanoparticles | |
| Holmes et al. | Cell sorting and separation using dielectrophoresis | |
| CN211859973U (en) | DEP power supply of air purification device adopting dielectrophoresis technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200923 Address after: Room d707, building D, Suzhou Institute of nanotechnology and nanobionics, Chinese Academy of Sciences, No.398 Ruoshui Road, Suzhou Industrial Park, Jiangsu Province, 215000 (room d707) Patentee after: Caike (Suzhou) Biotechnology Co.,Ltd. Address before: 215123 7th floor, building D, No. 398, Ruoshui Road, Suzhou Industrial Park, Suzhou City, Jiangsu Province Patentee before: SUZHOU TIANJI INNOVATION NANO TECHNOLOGY Co.,Ltd. |