CN210330611U - A device for collecting saliva from tiny insect larvae - Google Patents
A device for collecting saliva from tiny insect larvae Download PDFInfo
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- CN210330611U CN210330611U CN201920585212.9U CN201920585212U CN210330611U CN 210330611 U CN210330611 U CN 210330611U CN 201920585212 U CN201920585212 U CN 201920585212U CN 210330611 U CN210330611 U CN 210330611U
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- positive
- transverse plates
- saliva
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- vertical plate
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- 210000003296 saliva Anatomy 0.000 title claims abstract description 45
- 241000238631 Hexapoda Species 0.000 title claims abstract description 42
- QCHFTSOMWOSFHM-WPRPVWTQSA-N (+)-Pilocarpine Chemical compound C1OC(=O)[C@@H](CC)[C@H]1CC1=CN=CN1C QCHFTSOMWOSFHM-WPRPVWTQSA-N 0.000 abstract description 3
- QCHFTSOMWOSFHM-UHFFFAOYSA-N SJ000285536 Natural products C1OC(=O)C(CC)C1CC1=CN=CN1C QCHFTSOMWOSFHM-UHFFFAOYSA-N 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 229960001416 pilocarpine Drugs 0.000 abstract description 3
- 239000011324 bead Substances 0.000 description 8
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000007123 defense Effects 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 210000000214 mouth Anatomy 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 241001454907 Pteromalus puparum Species 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 238000010162 Tukey test Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000003128 head Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000920471 Lucilia caesar Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000001467 acupuncture Methods 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000003162 anti-predator Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000009340 pathogen transmission Effects 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
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- Peptides Or Proteins (AREA)
Abstract
The utility model provides a collection system of microminiature insect larva saliva: including the electroplax, be provided with positive pole and negative pole on the electroplax, be provided with the positive riser of being connected with the positive pole on the electroplax and the negative pole riser of being connected with the negative pole, positive riser is provided with at least three positive diaphragm in the equidistance towards negative pole riser one side, and the negative pole riser is provided with at least three negative pole diaphragm towards positive riser one side equidistance, and the positive diaphragm stretches into between two adjacent negative pole diaphragms, and the negative pole diaphragm stretches into between two adjacent positive pole diaphragms. The utility model discloses can be used for gathering some individual less and do not have the insect larva of obvious reaction to pilocarpine.
Description
Technical Field
The utility model belongs to the technical field of agricultural plant protection and specifically relates to a collection system of microminiature insect larva saliva.
Background
In order to effectively obtain nutrition, deal with toxic ingredients in food and develop new food resources, the oral cavity of the insect is accompanied with the adaptive characteristics of external and internal forms, and the physiological and biochemical functions of the insect are correspondingly adjusted. The components of insect saliva are quite complex and contain many important physiological functional factors. Insect saliva has many functions and properties, such as digestion, oral maintenance, water balance, pathogen transmission, acts as a carrier for pheromones, provides anti-predator defense, has antimicrobial activity, and can help circumvent animal host defense and plant host defense. Therefore, the research on insect saliva has important significance for the aspects of pest control and the like, and currently, saliva of insect larvae is generally stimulated by dissecting salivary glands or inducing pilocarpine, but for some larvae, the operation difficulty and the effect are not good.
Accordingly, there is a need for improvements in the art.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide an efficient collection system of microminiature insect larva saliva.
The utility model provides a collection system of microminiature insect larva saliva: comprises an electric plate;
the electric plate is provided with a positive electrode and a negative electrode, and is provided with a positive vertical plate connected with the positive electrode and a negative vertical plate connected with the negative electrode;
the anode vertical plate is provided with at least three anode transverse plates at equal intervals on one side facing the cathode vertical plate, and the cathode vertical plate is provided with at least three cathode transverse plates at equal intervals on one side facing the anode vertical plate;
the positive transverse plates extend into the space between two adjacent negative transverse plates; and the negative transverse plates extend into the space between two adjacent positive transverse plates.
As to the utility model discloses the collection system's of microminiature insect larva saliva improvement:
the positive transverse plates extend into the middle of the two adjacent negative transverse plates; the negative transverse plates extend into the middle of the two adjacent positive transverse plates.
As to the utility model discloses the collection system's of microminiature insect larva saliva further improvement:
the positive vertical plate and the negative vertical plate are parallel to each other.
As to the utility model discloses the collection system's of microminiature insect larva saliva further improvement:
the positive pole diaphragm is mutually perpendicular with positive pole riser, negative pole diaphragm and negative pole riser mutually perpendicular.
As to the utility model discloses the collection system's of microminiature insect larva saliva further improvement:
the distance between the adjacent anode transverse plates is equal to that between the adjacent cathode transverse plates; the number of the positive transverse plates is the same as that of the negative transverse plates.
The utility model discloses collection system of microminiature insect larva saliva's technical advantage does:
the utility model discloses can be simple convenient saliva collection that uses the current stimulation to realize single or a plurality of insect larva, the utility model discloses can be used for gathering some individual less and do not have the insect larva of obvious reaction to pilocarpine, can compensate prior art's defect. The utility model discloses realize structural improvement. If the structure is changed into the circuit electroplate like the utility model, the technical advantages of high flux and quick sampling can be brought. Conversely, if not improved, as in the prior art, the disadvantage of slower sampling speed and lower efficiency results.
Drawings
The following describes the present invention in further detail with reference to the accompanying drawings.
FIG. 1 is a schematic structural view of a device for collecting saliva of a microminiature insect larva according to the present invention;
FIG. 2 is a schematic view of the structure of FIG. 1 with insect larvae 4 placed thereon in practice;
FIG. 3 is an enlarged schematic view of the insect larvae 4 of FIG. 2 positioned between adjacent positive transverse plates 22 and negative transverse plates 32;
FIG. 4 is an electrophoretogram of comparative example 1 in which an equal number of small beads were collected at 1mA, 2.5mA and 4mA, respectively;
FIG. 5 is an electrophoretogram of comparative example 2 in which an equal number of beads were collected at 5s, 15s and 25s, respectively;
FIG. 6 is an electrophoretogram of comparative example 3 in which an equal number of small beads were collected under 2.5mA conditions in a phosphate buffer solution containing no PIC.
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto.
Example 1, collection system of microminiature insect larva saliva, is used for insect larva 4, as shown in fig. 1-3, includes a device that can stably provide electric current voltage (the utility model discloses the adoption is hua tuo brand's SDZ-II type electron acupuncture apparatus), an electric plate 1 that can provide steady electric current and voltage that comprises the interlude circuit, and electric plate 1 is PCB single face circuit board. The electric board is made of FR4 material and is 1.6mm thick, the circuit of the electric board is designed by adopting semi-process bare copper, and the width of the circuit is 0.25 mm.
One end of electroplax 1 is provided with anodal 2 and negative pole 3, is provided with anodal riser 21 of being connected with anodal 2 and the negative pole riser 31 of being connected with negative pole 3 on the electroplax 1, and anodal riser 21 and negative pole riser 31 are parallel to each other, and anodal riser 21 is provided with the anodal diaphragm 22 of a plurality of towards negative pole riser 31 one side equidistance, and negative pole riser 31 is provided with a plurality of negative pole diaphragm 32 towards anodal riser 21 one side equidistance. The positive transverse plate 22 is perpendicular to the positive vertical plate 21, and the negative transverse plate 32 is perpendicular to the negative vertical plate 31.
The distance between adjacent positive transverse plates 22 is equal to the distance between adjacent negative transverse plates 32, and the number of positive transverse plates 22 is equal to that of negative transverse plates 32. The positive transverse plates 22 (except the positive transverse plate 22 at one edge) extend into the middle of the two adjacent negative transverse plates 32, and the negative transverse plates 32 (except the negative transverse plate 32 at one edge) extend into the middle of the two adjacent positive transverse plates 22. On electroplax 1, positive pole diaphragm 22 and negative pole diaphragm 32 set up in turn, and 4 size regulations of insect larva that the interval between adjacent positive pole diaphragm 22 and the negative pole diaphragm 32 can detect as required, and the interval between adjacent positive pole diaphragm 22 and the negative pole diaphragm 32 equals. The middle distance is 0.3mm in the embodiment, and the saliva collecting device can be used for collecting the saliva of the pteromalus puparum and the blowfly puparum larva. When the insect larva breeding device is used, the two ends of the insect larva 4 are placed on the adjacent positive transverse plate 22 and the negative transverse plate 32, so that the two ends of the insect larva 4 are respectively contacted with the positive transverse plate 22 and the negative transverse plate 32.
The utility model discloses a use does:
the two ends of an insect larva 4 are placed on an adjacent positive transverse plate 22 and a negative transverse plate 32, a positive electrode 2 and a negative electrode 3 are connected with the SDZ-II type electronic needle therapy instrument, current sequentially passes through a positive electrode vertical plate 21, the positive transverse plate 22, the insect larva 4, the negative transverse plate 32 and a negative electrode vertical plate 31 from the positive electrode 2 and then enters the negative electrode 3, the current stimulates the insect larva 4, the insect larva 4 spits saliva after electric shock, small saliva liquid beads are formed around mouthparts, at the moment, a gun head is used for being close to the small saliva liquid beads to be collected, and the saliva of the insect larva 4 is stored in a phosphate buffer solution (pH 7.0) containing a protease Inhibitor (100 x), TRANSGENBIOTECH and China) and stored at the temperature of-80 ℃.
During the use process, for example, when the saliva of the pteromalus puparum and the lima puparum collecting larvae is collected in our laboratory, the method specifically comprises the following steps:
The method can avoid that a small amount of saliva globules are not easy to be absorbed by the gun head, and can also avoid that the saliva globules volatilize due to small amount. When the insect larva 4 is placed, attention needs to be paid to placing one end of the mouth tool upwards, and spit saliva droplets are prevented from being attached to an electric plate below the mouth tool.
Taking Lifly pupae collecting Cerebrophagus chinensis larva saliva collection as an example.
Comparative example 1: the current in step 1 and step 2 of example 1, which is "current 2.5 ma", was changed to "1 ma or 4 ma", and the rest was the same as example 1.
The data are expressed as mean±standard error(n＝5).one-way ANOVAand Tukey’s test(P<0.05).
FIG. 4: the same number of small beads were collected under 1mA, 2.5mA and 4mA, and the saliva samples were stained and photographed after 4-20% SDS-PAGE, to obtain the following pictures. As can be seen, the saliva protein species taken under different conditions were identical, but the protein content taken under 1mA was lower.
Comparative example 2: the "holding current 15 s" in step 2 of example 1 was changed to "5 s or 25 s", and the rest was the same as example 1.
The data are expressed as mean±standard error(n＝5).one-way ANOVAand Tukey’s test(P<0.05).
FIG. 5: the same number of beads were collected at 5s, 15s and 25s, respectively, and saliva samples were stained and photographed after 4-20% SDS-PAGE to obtain the following images. As can be seen, the saliva protein species taken under different conditions were identical, but the protein content taken under 5s conditions was lower.
Comparative example 3: the protease inhibitor in step 3 was eliminated and the rest was identical to example 1.
If the protease inhibitor in the step 3 is eliminated, the protein in the saliva taken by us can be decomposed by the protease in the saliva or outside, and the later experiment is influenced.
FIG. 6: an equal number of beads were collected under 2.5mA, and stored in phosphate buffer containing protease inhibitor (abbreviated as "PIC") and not containing PIC, and saliva samples were stained and photographed after 4-20% SDS-PAGE, to obtain the following images. As can be seen from the figure, in the phosphate buffer without PIC, the saliva sample was degraded by protease, which affected the later experimental results.
Finally, it is also noted that the above-mentioned list is only a few specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many modifications are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the invention should be considered as within the scope of the invention.
Claims (5)
1. Collection system of microminiature insect larva saliva, its characterized in that: comprising an electric plate (1);
the anode (2) and the cathode (3) are arranged on the electric plate (1), and the anode vertical plate (21) connected with the anode (2) and the cathode vertical plate (31) connected with the cathode (3) are arranged on the electric plate (1);
at least three anode transverse plates (22) are arranged on one side of the anode vertical plate (21) facing the cathode vertical plate (31) at equal intervals, and at least three cathode transverse plates (32) are arranged on one side of the cathode vertical plate (31) facing the anode vertical plate (21) at equal intervals;
the positive transverse plates (22) extend into the space between two adjacent negative transverse plates (32); the negative pole transverse plates (32) extend into the space between two adjacent positive pole transverse plates (22).
2. The device for collecting saliva of microminiature insect larvae, according to claim 1, wherein:
the positive transverse plates (22) extend into the middle of the two adjacent negative transverse plates (32); the negative transverse plates (32) extend into the middle of the two adjacent positive transverse plates (22).
3. The device for collecting saliva of microminiature insect larvae according to claim 2, wherein:
the anode vertical plate (21) and the cathode vertical plate (31) are parallel to each other.
4. The device for collecting saliva of microminiature insect larvae, according to claim 3, wherein:
the positive transverse plate (22) is perpendicular to the positive vertical plate (21), and the negative transverse plate (32) is perpendicular to the negative vertical plate (31).
5. The device for collecting saliva of microminiature insect larvae, according to claim 4, wherein:
the distance between the adjacent positive transverse plates (22) is equal to the distance between the adjacent negative transverse plates (32); the number of the positive transverse plates (22) is the same as that of the negative transverse plates (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920585212.9U CN210330611U (en) | 2019-04-26 | 2019-04-26 | A device for collecting saliva from tiny insect larvae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920585212.9U CN210330611U (en) | 2019-04-26 | 2019-04-26 | A device for collecting saliva from tiny insect larvae |
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| Publication Number | Publication Date |
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| CN210330611U true CN210330611U (en) | 2020-04-17 |
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| CN201920585212.9U Active CN210330611U (en) | 2019-04-26 | 2019-04-26 | A device for collecting saliva from tiny insect larvae |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984774A (en) * | 2019-04-26 | 2019-07-09 | 浙江大学 | The acquisition device and method of microminiature insect larvae saliva |
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2019
- 2019-04-26 CN CN201920585212.9U patent/CN210330611U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109984774A (en) * | 2019-04-26 | 2019-07-09 | 浙江大学 | The acquisition device and method of microminiature insect larvae saliva |
| CN109984774B (en) * | 2019-04-26 | 2024-07-23 | 浙江大学 | Device and method for collecting saliva of micro-insect larvae |
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