CN210314283U

CN210314283U - Nucleic acid isothermal amplification mechanism and synchronous nucleic acid isothermal amplification instrument

Info

Publication number: CN210314283U
Application number: CN201921256852.1U
Authority: CN
Inventors: 王升启; 肖瑞; 荣振
Original assignee: Institute of Pharmacology and Toxicology of AMMS
Current assignee: Institute of Pharmacology and Toxicology of AMMS
Priority date: 2019-08-05
Filing date: 2019-08-05
Publication date: 2020-04-14
Anticipated expiration: 2029-08-05

Abstract

The utility model provides a nucleic acid isothermal amplification mechanism, which comprises a base and a reaction tube rack, a light source, a detector and a guide tube arranged on the base; the light source and the detector are respectively located on both sides of the reaction tube rack; There are several accommodating grooves on the pipe frame, and the side walls of the accommodating grooves are provided with an incident through hole and an exit through hole, and the light from the light source can pass through the incident through hole, the accommodating groove, and the exit through hole in sequence to reach the detector; A guide tube is arranged between the incident through hole and the light source, so that light only enters the incident through hole from the axial direction of the guide tube. The nucleic acid isothermal amplification mechanism of the utility model uses a guiding tube to connect the light source and the reaction tube rack, so as to prevent the extra light of the light source from entering the accommodating groove and causing light interference, and simultaneously introducing light into multiple accommodating grooves, the lighting conditions are stable and balanced, and the detection The results are reliable. On this basis, the utility model also provides a synchronous nucleic acid isothermal amplifier.

Description

Nucleic acid isothermal amplification mechanism and synchronous nucleic acid isothermal amplification instrument

Technical Field

The utility model relates to a nucleic acid amplification technical field particularly, relates to a nucleic acid isothermal amplification mechanism and synchronous formula nucleic acid isothermal amplification appearance.

Background

Detection techniques based on isothermal nucleic acid amplification have been developed rapidly in recent years. Isothermal technologies of various mechanisms are emerging, and some technologies are already mature, complete the transition from laboratories to practical applications, and are gradually and widely applied in the fields of molecular biology, medicine, law and the like. Particularly, in the aspect of clinical and on-site (point-of-care) rapid diagnosis technology, the isothermal amplification technology of nucleic acid shows outstanding superiority. More importantly, the isothermal nucleic acid amplification technology does not need time process of temperature change and gets rid of dependence on fine instruments and equipment, so that the detection and diagnosis of pathogens can be realized quickly and in high flux.

In the isothermal amplification of nucleic acids, the solution undergoes an isothermal amplification reaction over time. In the process, the solution is periodically irradiated by laser, the intensity of the laser is weakened after the laser passes through the solution, and reaction data of isothermal amplification of the solution can be obtained by instruments such as photocell detection and the like.

However, in the existing nucleic acid isothermal amplification instrument, a plurality of reaction tube accommodating grooves correspond to a set of illumination equipment, and the light emission direction of the illumination equipment is not focused, which is easy to cause additional illumination interference, i.e., part of light passes through the reaction tube, and part of light, such as light diffusely reflected by a light source, only enters the reaction tube and cannot pass through the reaction tube to reach the detection instrument.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a nucleic acid isothermal amplification mechanism, it can avoid the incident ray among the lighting apparatus only to shine into the reaction tube and unable outgoing arrival detector.

Another object of the present invention is to provide a synchronous isothermal nucleic acid amplification device, which can perform isothermal amplification detection on the nucleic acid in the reaction tube in a plurality of storage tanks in a whole cover, and the interference of nucleic acid amplification is small, and the detection result is reliable.

The embodiment of the utility model is realized like this:

a nucleic acid isothermal amplification mechanism comprises a base, a reaction tube rack, a light source, a detector and a guide tube barrel, wherein the reaction tube rack, the light source, the detector and the guide tube barrel are arranged on the base; the light source and the detector are respectively positioned at two sides of the reaction tube rack; the reaction tube frame is provided with a plurality of accommodating grooves, the side walls of the accommodating grooves are provided with incident through holes and emergent through holes, and light rays from the light source can sequentially pass through the incident through holes, the accommodating grooves and the emergent through holes to reach the detector; a guide tube barrel is arranged between the incident through hole and the light source.

The guide tube barrel is positioned between the incident through hole and the light source, light rays emitted from the light source enter the accommodating groove through the incident through hole only by being guided by the guide tube barrel, and the light rays entering the incident through hole only come from the guide tube barrel, so that the statistical interference caused by the incidence of other light rays of the light source is prevented; and a plurality of accommodating grooves are arranged on the reaction tube frame, the accommodating grooves are used for accommodating the reaction tubes, each accommodating groove corresponds to one guide tube barrel, one incident through hole and one emergent through hole, and light rays of the light source can simultaneously pass through all the reaction tubes, so that the effect of synchronous detection is realized.

In a preferred embodiment of the present invention, the light source is a light emitting plate. The technical effects are as follows: the illumination of the flat-plate-shaped light source is strong in each direction and is matched with the guide pipe barrel for use, the illumination intensity received by the reaction pipes in the containing grooves is consistent, and isothermal amplification detection is more accurate and more beneficial to uniform comparison; and the tabular light source can perform illumination action on the reaction tubes of the plurality of accommodating grooves, and the reaction tubes of the accommodating grooves do not need to be respectively illuminated by moving the light source one by one. Meanwhile, the influence of heat generation and heat dissipation of the flat-plate-shaped light source on the nucleic acid solution is smaller and more balanced in consideration of the heat dissipation of the light source.

In a preferred embodiment of the present invention, the reaction tube rack is detachably disposed on the base. The technical effects are as follows: the reaction tube support is used for loading the reaction tube, the incident through hole position and the emergent through hole position which correspond to the reaction tube support are generally unchanged, but the sizes of the corresponding reaction tubes can be inconsistent, when the reaction tubes are smaller, the reaction tube support with the smaller accommodating groove can be installed, and when the reaction tubes are larger, the reaction tube support with the larger accommodating groove can be installed. The detachable reaction pipe support enables the nucleic acid isothermal amplification mechanism to be more practical and can meet the detection work of reaction pipes with different sizes.

In a preferred embodiment of the present invention, the heating device further comprises a heater; the heater is flat and is attached to the reaction tube rack. The technical effects are as follows: the heater is used for providing constant ambient temperature for all the reaction tubes in the accommodating groove, and the flat-plate-shaped heater is favorable for heat dissipation to generate constant temperature which is the same for all the reaction tubes.

In a preferred embodiment of the present invention, the heater is attached to one side of the reaction tube rack close to the detector, and the heater is provided with a transparent through hole corresponding to the position of the exit through hole. The technical effects are as follows: the heater is arranged on one side close to the detector, and unnecessary influence on the light source caused by heat of the heater can be avoided. The heater is provided with the light-transmitting through hole, so that the area of the heater can be enlarged as much as possible, and the reaction tube in the accommodating groove is uniformly heated.

In a preferred embodiment of the present invention, the heat insulation board further comprises a first heat insulation board; the first heat insulation plate is positioned between the light source and the guide tube barrel, and a light-transmitting through hole is formed in the position, corresponding to the incident through hole, of the first heat insulation plate. The technical effects are as follows: the first heat insulation plate is positioned between the light source and the guide pipe barrel, so that the influence of the heat of the heater on the light source is reduced.

In a preferred embodiment of the present invention, the guide tube is disposed on a side of the first heat insulation plate facing the reaction tube rack. The technical effects are as follows: the guide pipe barrel is connected with the first heat insulation plate, so that the guide pipe barrel is prevented from directly contacting the reaction pipe frame, and the installation stability of the guide pipe barrel is not influenced by expansion with heat and contraction with cold in the temperature change process of the reaction pipe frame. Simultaneously, the reaction tube rack is independently and detachably arranged.

In a preferred embodiment of the present invention, the heat insulation board further comprises a second heat insulation board; the second heat insulation plate is located between the detector and the reaction tube rack, and a light-transmitting through hole is formed in the position, corresponding to the emergent through hole, of the second heat insulation plate. The technical effects are as follows: the second heat insulation plate is positioned between the detector and the reaction tube rack, so that the influence of the heat of the heater on the detector is reduced.

A synchronous nucleic acid isothermal amplification instrument comprises a cover body and the nucleic acid isothermal amplification mechanism, wherein the nucleic acid isothermal amplification mechanism is arranged in the cover body.

Because the isothermal amplification instrument needs to measure the attenuation value of the illumination intensity, after the cover body is arranged, the illumination not only avoids internal interference due to the arrangement of the guide tube barrel, but also avoids light interference from the outside due to the isolation of the cover body from external illumination, thereby further improving the accuracy and reliability of nucleic acid amplification detection.

In the preferred embodiment of the present invention, the utility model further comprises a buckle cover; the buckle cover is arranged on the cover body corresponding to the reaction tube rack. The technical effects are as follows: the setting of buckle closure, when needing to change the reaction tube or change the reaction tube support, only need open the buckle closure and need not to lift off whole cover body, further reduced the influence of outside illumination and temperature, improved nucleic acid isothermal amplification's detection efficiency.

The embodiment of the utility model provides a beneficial effect is:

the utility model discloses a nucleic acid isothermal amplification mechanism, utilize the structure of direction bobbin connection light source and reaction tube support, the inner chamber of usable direction bobbin realizes that light switches on and utilizes its outer tube to realize separating of light and keep off, make the light that comes from the light source only be on a parallel with the part of direction bobbin axis direction can get into the storage tank and absorbed by the reaction tube via the inner chamber of direction bobbin, and other light that is not parallel with direction bobbin axis direction can be separated like the light that the light source goes out the diffuse reflection and sends, unnecessary interference light has been avoided, the numerical value that weakens of light intensity has more accurately been embodied.

The utility model discloses a synchronous formula nucleic acid isothermal amplification appearance after the illumination of having stopped the internal portion of cover is interfered, further reduces the illumination influence of the external portion of cover, has improved the degree of accuracy and the reliability that nucleic acid amplification detected.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic perspective view of a first view angle of an isothermal nucleic acid amplification mechanism according to an embodiment of the present invention;

FIG. 2 is a schematic perspective view of a second perspective view of the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention;

FIG. 3 is a top view of the isothermal nucleic acid amplification mechanism according to an embodiment of the present invention;

FIG. 4 is a front view of an isothermal nucleic acid amplification mechanism according to an embodiment of the present invention;

FIG. 5 is a rear view of the isothermal nucleic acid amplification mechanism according to an embodiment of the present invention;

FIG. 6 is a schematic diagram illustrating a first view structure of a reaction tube rack and a heater in the isothermal nucleic acid amplification mechanism according to an embodiment of the present invention;

FIG. 7 is a schematic diagram illustrating a second perspective structure of a reaction tube rack and a heater in the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention;

FIG. 8 is a schematic diagram of a first view structure of a reaction tube rack in the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention;

FIG. 9 is a perspective view of a synchronous isothermal nucleic acid amplification apparatus according to an embodiment of the present invention;

FIG. 10 is a schematic diagram of the internal structure of the simultaneous isothermal nucleic acid amplification apparatus according to the embodiment of the present invention.

In the figure: 1-a base; 2-a reaction tube rack; 3-a light source; 4-a detector; 5-a containing groove; 6-incident through holes; 7-an exit via; 8-a guide tube barrel; 9-a heater; 10-a first insulation board; 11-a second insulation board; 12-a cover body; 13-buckling a cover; 14-a display screen; 15-handle.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of the embodiments of the present invention, as generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Some embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

FIG. 1 is a schematic perspective view of a first view angle of an isothermal nucleic acid amplification mechanism according to an embodiment of the present invention; FIG. 2 is a schematic perspective view of a second perspective view of the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention; FIG. 3 is a top view of the isothermal nucleic acid amplification mechanism according to an embodiment of the present invention; FIG. 4 is a front view of an isothermal nucleic acid amplification mechanism according to an embodiment of the present invention; FIG. 5 is a rear view of the isothermal nucleic acid amplification mechanism according to an embodiment of the present invention; FIG. 6 is a schematic diagram showing a first perspective structure of the reaction tube holder 2 and the heater 9 in the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention; FIG. 7 is a schematic diagram showing a second perspective structure of the reaction tube holder 2 and the heater 9 in the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention; FIG. 8 is a schematic diagram of a first view structure of the reaction tube holder 2 in the isothermal nucleic acid amplification mechanism according to the embodiment of the present invention.

The first embodiment:

referring to fig. 1 to 8, the present embodiment provides a nucleic acid isothermal amplification mechanism, which includes a base 1, a reaction tube rack 2 disposed on the base 1, a light source 3, and a detector 4; the light source 3 and the detector 4 are respectively positioned at two sides of the reaction tube rack 2.

Referring to fig. 1 to 3 and 6 to 8, a plurality of accommodating grooves 5 are formed in the reaction tube frame 2, an incident through hole 6 and an exit through hole 7 are formed in a side wall of any one of the accommodating grooves 5, and light from the light source 3 can sequentially pass through the incident through hole 6, the accommodating groove 5 and the exit through hole 7 to reach the detector 4. Wherein, as shown in the figure, the containing groove 5 is arranged on the reaction tube frame 2, the opening faces upwards, the right side wall is provided with an incident through hole 6, and the left side wall is provided with an emergent through hole 7.

Referring to fig. 2, a plurality of guide pipe barrels 8 are further provided; a guide tube 8 is provided between any one of the incident through holes 6 and the light source 3 so that light is incident into the incident through hole 6 only from the axial direction of the guide tube 8. The light propagation route is as follows: the light source 3, the guide pipe barrel 8, the incident through hole 6, the accommodating groove 5, the emergent through hole 7 and the detector 4.

The working principle of the nucleic acid isothermal amplification mechanism of the embodiment is as follows: the light source 3 penetrates through the solution in the reaction tube in the accommodating groove 5 from the path, the light intensity of the light source is reduced by the solution in the reaction process, the data of the change of the light intensity along with the reaction time in the solution reaction process can be obtained according to the light intensity measured by the detector 4, a corresponding curve graph is further manufactured, and the process of the isothermal amplification of the nucleic acid is visually represented.

In the isothermal nucleic acid amplification mechanism of the present embodiment, the guide tube 8 is disposed between the incident through hole 6 and the light source 3, and the receiving groove 5, the exit through hole 7 and the detector 4 are disposed at the front end of the incident through hole 6 in sequence, so that the light emitted from the light source 3 enters the receiving groove 5 through the incident through hole 6 only by being guided by the guide tube 8. Moreover, by adjusting the distance between the guide tube barrel 8 and the incident through hole 6, the inner cavity of the guide tube barrel 8 can be used for guiding, and the outer barrel of the guide tube barrel can be used for blocking light rays, so that the light rays entering the incident through hole 6 only come from the guide tube barrel 8, and the light rays without diffuse scattering enter the incident through hole 6, thereby preventing other light rays of the light source 3 from entering to cause statistical interference; and a plurality of accommodating grooves 5 are arranged on the reaction tube frame 2, wherein the accommodating grooves 5 are used for accommodating reaction tubes, and each accommodating groove 5 corresponds to one guide tube barrel 8, one incident through hole 6 and one emergent through hole 7, so that the light of the light source 3 can simultaneously pass through all the reaction tubes, and the effect of synchronous detection is realized.

Second embodiment:

referring to FIGS. 1 to 4, the present embodiment provides a nucleic acid isothermal amplification mechanism, which is substantially the same as the nucleic acid isothermal amplification mechanism of the first embodiment, and the difference between the two embodiments is that the light source 3 of the nucleic acid isothermal amplification mechanism of the present embodiment is a light emitting flat plate.

The light-emitting flat plate can be a flat plate with uniform light-emitting brightness, small heat productivity and long service life, such as an LED.

The third embodiment:

referring to FIGS. 1 to 3 and 6 to 8, the present embodiment provides a nucleic acid isothermal amplification mechanism, which is substantially the same as the nucleic acid isothermal amplification mechanisms of the first and second embodiments, except that the reaction tube holder 2 of the nucleic acid isothermal amplification mechanism of the present embodiment is detachably disposed on the base 1.

The reaction tube holder 2 can be inserted into the base 1, or inserted into a position between the light source 3 and the detector 4, or fixed by a screw, a plug, or other tools.

The fourth embodiment:

referring to fig. 2, 3, 6 and 7, the present embodiment provides a nucleic acid isothermal amplification mechanism, which is substantially the same as the nucleic acid isothermal amplification mechanisms of the first, second and third embodiments, and the difference between them is that the nucleic acid isothermal amplification mechanism of the present embodiment further includes a heater 9; the heater 9 is flat and attached to the reaction tube frame 2.

Further, a heater 9 is attached to one side of the reaction tube frame 2 close to the detector 4, and a light-transmitting through hole is arranged at the position of the heater 9 corresponding to the emergent through hole 7.

The heater 9 may be positioned at the bottom of the reaction tube holder 2 and may be in a half-surrounded or sandwiched state. The heater 9 may be provided with a thermal insulation layer on the outside.

Fifth embodiment:

referring to fig. 1 to 3, the present embodiment provides a nucleic acid isothermal amplification mechanism, which is substantially the same as the nucleic acid isothermal amplification mechanisms of the first, second, third and fourth embodiments, and the difference between the nucleic acid isothermal amplification mechanism of the present embodiment further includes a first heat shield 10; the first heat insulating plate 10 is positioned between the light source 3 and the guide tube 8, and the first heat insulating plate 10 is provided with a light-transmitting through-hole at a position corresponding to the incident through-hole 6.

Further, a guide tube 8 is provided on the side of the first heat shield 10 facing the reaction tube rack 2.

Further, a second heat insulation plate 11 is also included; the second heat insulation board 11 is positioned between the detector 4 and the reaction tube frame 2, and a light-transmitting through hole is arranged at the position of the second heat insulation board 11 corresponding to the emergent through hole 7.

The first heat insulation board 10 and the second heat insulation board 11 are respectively provided with through holes for transmitting light at positions corresponding to the incident through hole 6 and the emergent through hole 7, so that the areas of the first heat insulation board 10 and the second heat insulation board 11 are enlarged as much as possible, and meanwhile, the installation stability of other related structures is improved. At this time, the reaction tube holder 2 may be insertedly installed between the first insulation plate 10 and the second insulation plate 11.

Sixth embodiment:

referring to fig. 9 and 10, the present embodiment provides a synchronous isothermal nucleic acid amplification apparatus, which includes a cover 12 and the isothermal nucleic acid amplification mechanism of any of the above embodiments, wherein the isothermal nucleic acid amplification mechanism is embedded in the cover 12. Further, a buckle cover 13 is also included; the cover 13 is provided on the cover body 12 at a position corresponding to the reaction tube holder 2.

In addition, the housing 12 may be provided with a display 14 for direct display of sensed data and charts. Meanwhile, in order to facilitate moving and carrying, the cover 12 should be provided with a handle 15.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A nucleic acid isothermal amplification mechanism is characterized by comprising a base, a reaction tube rack, a light source, a detector and a guide tube barrel, wherein the reaction tube rack, the light source, the detector and the guide tube barrel are arranged on the base;

the light source and the detector are respectively positioned at two sides of the reaction tube rack;

the reaction tube frame is provided with a plurality of accommodating grooves, the side walls of the accommodating grooves are provided with incident through holes and emergent through holes, and light rays from the light source can sequentially pass through the incident through holes, the accommodating grooves and the emergent through holes to reach the detector;

and one guide tube barrel is arranged between the incident through hole and the light source.

2. The isothermal nucleic acid amplification mechanism of claim 1, wherein the light source is a light emitting plate.

3. The isothermal nucleic acid amplification mechanism according to claim 1, wherein the reaction tube holder is detachably provided to the base.

4. The nucleic acid isothermal amplification mechanism according to claim 1, further comprising a heater; the heater is flat and is attached to the reaction tube rack.

5. The isothermal nucleic acid amplification mechanism according to claim 4, wherein the heater is attached to one side of the reaction tube holder close to the detector, and the heater is provided with a light-transmitting through hole at a position corresponding to the exit through hole.

6. The nucleic acid isothermal amplification mechanism according to claim 1, further comprising a first heat insulating plate; the first heat insulation plate is positioned between the light source and the guide tube barrel, and a light-transmitting through hole is formed in the position, corresponding to the incident through hole, of the first heat insulation plate.

7. The isothermal nucleic acid amplification mechanism according to claim 6, wherein the guide tube barrel is disposed on a side of the first heat insulation plate facing the reaction tube holder.

8. The nucleic acid isothermal amplification mechanism according to claim 1, further comprising a second heat insulating plate; the second heat insulation plate is located between the detector and the reaction tube rack, and a light-transmitting through hole is formed in the position, corresponding to the emergent through hole, of the second heat insulation plate.

9. A synchronous isothermal nucleic acid amplification instrument, comprising a cover and the isothermal nucleic acid amplification mechanism according to any one of claims 1 to 8, wherein the isothermal nucleic acid amplification mechanism is embedded in the cover.

10. The simultaneous nucleic acid isothermal amplification instrument according to claim 9, further comprising a cover; the buckle cover is arranged on the cover body corresponding to the reaction tube rack.