CN210163462U - Vertical rotary tank bioreactor for large-scale cell culture - Google Patents
Vertical rotary tank bioreactor for large-scale cell culture Download PDFInfo
- Publication number
- CN210163462U CN210163462U CN201920581654.6U CN201920581654U CN210163462U CN 210163462 U CN210163462 U CN 210163462U CN 201920581654 U CN201920581654 U CN 201920581654U CN 210163462 U CN210163462 U CN 210163462U
- Authority
- CN
- China
- Prior art keywords
- tank
- culture tank
- culture
- cover
- rotary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000012007 large scale cell culture Methods 0.000 title claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 26
- 230000010412 perfusion Effects 0.000 claims abstract description 5
- 238000001514 detection method Methods 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000003381 stabilizer Substances 0.000 claims description 14
- 238000005273 aeration Methods 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000005192 partition Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 7
- 238000009423 ventilation Methods 0.000 abstract description 6
- 230000003321 amplification Effects 0.000 abstract description 3
- 238000007885 magnetic separation Methods 0.000 abstract description 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 3
- 238000002659 cell therapy Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 238000001890 transfection Methods 0.000 abstract description 2
- 238000007599 discharging Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 19
- 239000001963 growth medium Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 238000009580 stem-cell therapy Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a vertical rotary tank bioreactor for large-scale cell culture, which comprises a rotatable culture tank, wherein a tank cover is arranged at the tank opening of the culture tank in a sealing way; a rotary driving device is arranged below the bottom of the culture tank and can drive the culture tank to rotate along the vertical central axis of the culture tank; the cover of the culture tank is fixed and does not rotate, a plurality of connectors are arranged on the cover, a rotary joint is arranged between the cover and the tank opening, and the rotary joint realizes the closed rotation of the culture tank; the interface on the tank cover is also connected with an electrode, a feeding/discharging pipeline, an on-line cell detection system, a stirring system, a perfusion system and a ventilation system. The utility model discloses be suitable for whole preparation process of cell therapy preparation, including cell separation, magnetic separation transfection, amplification, concentration, washing, dispose the partial shipment all can be in the utility model discloses a accomplish in totally enclosed, full automatic control's the bioreactor.
Description
Technical Field
The utility model relates to a vertical rotary tank bioreactor for large-scale cell culture, which belongs to the technical field of cell culture and regenerative medicine.
Background
In vitro culture of cells and tissues has become an essential element in life research and practice. The traditional bioreactor can only culture cells and harvest proteins, and is rarely used for harvesting cells. With the rise of immunotherapy and stem cell therapy, a large-scale, totally-enclosed, fully-automatic and multifunctional cell preparation device is a delay.
Disclosure of Invention
In order to solve the existing problems, the utility model discloses a rotary bioreactor for large-scale culture.
The utility model discloses a concrete scheme does:
a vertical rotary bioreactor for large-scale culture comprises a rotatable culture tank, wherein a tank cover is arranged at the tank opening of the culture tank in a sealing manner; a rotary driving device is arranged below the bottom of the culture tank and can drive the culture tank to rotate along the vertical central axis of the culture tank; cultivate the fixed irrotational of jar cover, it has a plurality of interface to open on the cover, be provided with rotary joint between cover and the jar mouth, rotary joint realizes the airtight rotation of culture tank.
The interface on the tank cover is also connected with different types of electrodes, a material inlet/outlet pipeline, an online cell detection system, a stirring system, a perfusion system and a ventilation system.
The bioreactor is arranged in a thermostat, an observation window is arranged on a door of the thermostat, a partition plate or glass made of transparent materials is arranged in the observation window, and the thermostat is further provided with a constant temperature system and a temperature display system.
The bottom of the culture tank is provided with a cross groove which is crossed, and the intersection point of the cross groove is positioned at the center of the tank bottom.
The rotary driving device comprises a rotary tray and a bottom motor, the upper surface of the rotary tray is provided with a cross convex strip in a cross shape, the intersection point of the cross convex strip is located at the center of the rotary tray, and a cross groove at the lower part of the rotary tank body is arranged on the cross convex strip of the rotary tray and is just inserted into the cross groove in an inosculation manner.
The center of the rotary tray is provided with a connecting sleeve extending downwards, and the connecting sleeve is connected with a driving shaft of a bottom motor.
The bottom motor is arranged in the base, a bearing is arranged between the rotary tray and the base, the rotary tray is driven by the bottom motor to synchronously rotate with the culture tank, and the base is kept still.
An upper supporting plate is further arranged above the tank cover, a plurality of stabilizers are uniformly arranged on the upper supporting plate, and the stabilizers are connected with compressed air; when the culture tank rotates, the central shaft of the stabilizer descends to press the tank cover, and the air pressure is adjustable to stabilize the rotating culture tank.
The upper supporting plate is connected with the upper ends of the side plates through screws, and the lower ends of the side plates are fixed with the base;
the cover has been seted up a plurality of connecting hole, it is equipped with the bolt to go up the backup pad, and the bolt inserts in the cover connecting hole in order to prevent the cover rotatory.
The bottom of the culture tank is provided with a circle of concentric flow gathering grooves, a flow guide pipe is correspondingly arranged 2-3mm above the groove and is mounted on the tank cover, the periphery of the bottom of the culture tank faces the flow gathering grooves, and slopes inclining downwards are arranged from the center of the culture tank to the flow gathering grooves.
The aeration system comprises an air supply end valve, a flowmeter, a filter and an aeration pipeline which is positioned in the axial direction and penetrates through the stirring shaft, and the tail end of the aeration pipeline is provided with a micro-bubble generator; and the upper end of the vent pipe is provided with an air inlet passage connected with the vent pipe.
The culture tank is in a cylindrical shape, the top of the culture tank is in a circular bead shape, the top of the culture tank is closed to form a tank opening with a necking, a stirring shaft is arranged in the culture tank, the stirring shaft is located in the axial center of the culture tank, stirring paddles are arranged on the position, close to the lower end of the stirring shaft, the top end of the stirring shaft is connected with a top motor, and the top.
The lower part of the constant temperature box is provided with a roller, and the roller is provided with a brake plate.
The utility model discloses a theory of operation is:
the utility model is a complete bioreactor for cell culture, and integrates the function of a centrifuge. Under the condition that the tank body is not rotated, the tank body is a complete bioreactor, animal and plant cells and microorganisms can be cultured, when the bottom motor is started, the tank body can be rotated at high speed, an online centrifuge is formed, and the cultured cells can be centrifugally separated, concentrated and washed.
The utility model has the advantages that:
the utility model is suitable for a whole preparation process of cell therapy preparation, including cell separation, magnetic separation transfection, amplification, concentration, washing, configuration to the partial shipment all be in the utility model discloses a accomplish in totally enclosed, full automatic control's the bioreactor.
The utility model discloses save the production link, avoided the pollution.
The utility model discloses possess the function of extensive, totally closed, full-automatic, three-dimensional culture cell, still possess functions such as cell concentration, cell washing, cell detection, cell preparation, preparation automatic encapsulation simultaneously.
Drawings
Figure 1 is the overall internal structure schematic diagram (also as abstract figure) of the utility model,
figure 2 is a schematic diagram of the overall external structure of the present invention,
figure 3 is an enlarged view of a portion of figure 1 at B,
reference numerals: the device comprises a base, a bottom motor, a bearing, a rotating tray, a stirring blade, a culture tank, a guide pipe, a stirring shaft, a rotating joint, a tank cover, a fixing upper cover, a stabilizer, a ventilating system, a manual bolt, a side plate, a roller, a viewing window, a cross groove, a handle, a collecting groove, a connecting sleeve and a cross convex strip, wherein the base is 1, the bottom motor is 2, the bearing is 3, the rotating tray is 4, the stirring blade is 5, the culture tank is 6, the guide pipe is 7, the stirring shaft is 8, the rotating joint is.
Detailed Description
The invention will be further elucidated with reference to the drawings and the detailed description. It should be understood that the following detailed description is illustrative of the invention only and is not intended to limit the scope of the invention.
The first embodiment is as follows:
FIG. 1 is the whole internal structure of the utility model, it can be seen from the attached drawings that the utility model is used for the vertical rotary bioreactor for large-scale cultivation, including culture tank 6, in practical application, culture tank 6 is vertical to be put, cultivates the cell in culture tank 6. In the culture process, the upper stirring motor drives the stirring paddle to rotate at a low speed, and the culture medium is stirred to be uniformly mixed. The rotary driving device is arranged below the bottom of the culture tank 6, and can drive the culture tank 6 to rotate at a high speed along the vertical central axis of the culture tank to generate centrifugal force, so that cells can be separated, concentrated and washed. The rotation of the culture tank 6 is not available in any culture tank 6 at present, the utility model combines the functions of the culture tank and the centrifuge, and is one of the most important utility model points. The utility model discloses a culture tank 6 can be applicable to the volume and be 2L and the extensive cultivation more than 2L.
For example, in CAR-T cell culture, the utility model discloses culture tank high speed rotation is equipped with the micro-imaging system and can separates the leucocyte from the plasma, and then with the leucocyte feedback jar in that collects, through steps such as magnetic separation, modification, amplification, can harvest the cell. When the cells are harvested, the culture tank can be rotated at a high speed to centrifuge the fermentation liquid, after the centrifugation is finished, the culture medium is discharged out of the collection tank by air pressure, then normal saline is added for cleaning for 1-2 times, finally the dosage is proportioned according to the number of cells per milliliter and the proportion of the normal saline and the frozen stock solution, the cells are uniformly stirred, a sampling system is started for sampling, and then the cells are subpackaged and frozen. The whole process is totally closed in the bioreactor, and the full automation is completed, which is not available in the prior equipment.
Example two:
fig. 2 is a schematic example of the whole external structure of the utility model, and it can be seen from the attached drawing that the culture tank 6 is arranged in the incubator, the observation window 17 has been seted up to the lateral wall of the incubator, and the baffle or the glass of transparent material are arranged in the observation window 17, and the incubator is also provided with constant temperature system and temperature display system. One side of the incubator is a door that can be opened by rotation, on which a handle 19 is provided, said viewing window 17 being located on the door or on another side wall of the incubator.
Example three:
this example describes in detail the specific means for achieving the rotation of the culture tank 6 driven by the bottom motor 2, and the high speed rotation, and the sealing connection of the tank mouth. Fig. 3 is a partial enlarged view of the part B in fig. 1, and it can be seen from the attached drawing that a crisscross cross groove 18 is formed in the bottom of the culture tank 6, the rotary driving device includes a rotary tray 4 and a bottom motor 2, a crisscross cross raised strip 22 is arranged on the upper surface of the rotary tray 4, the intersection point of the raised strip 22 is located at the center of the rotary tray 4, the rotary tray 4 is arranged below the thickened base in a contacting manner, and the raised strip 22 is just inserted into the crisscross groove 18 in an inosculating manner. The intersection of the cross grooves 18 is located in the center of the thickened base. By inserting the cross ribs 22 into the cross grooves 18, it is achieved that the culture tank 6 and the rotating tray 4 are held synchronously fixed in the horizontal direction without any positional deviation. The culture tank 6 and the fixing upper cover 11 and the side plate 15 at the bottom of the culture tank 6 are fixed in the vertical direction, so that the height is not deviated.
Example four:
in this example, the center of the rotating tray 4 is provided with a connecting sleeve 21 extending downwards, and the connecting sleeve 21 is sleeved on and covers the driving shaft of the bottom motor 2; the connecting sleeve 21 is sleeved and surrounded around the driving shaft of the bottom motor 2, so that the bottom motor 2 can be stably connected with and driven by the rotating tray 4.
The below of culture tank 6 is provided with base 1, and bottom motor 2 is located inside base 1, and bottom motor 2's drive shaft is connected with the center of rotatory tray 4, is provided with bearing 3 between rotatory tray 4 and the base 1, and bottom motor 2 drive rotatory tray 4 and culture tank 6 synchronous revolution, and base 1 keeps motionless. The base 1 supports the culture tank 6 above the bottom motor 2, providing a support point, while the culture tank 6 is to be able to rotate, while the base 1 is kept stationary all the time.
Example five:
this example is an embodiment for realizing a specific connection of the base 1 to the rotating tray 4: the bearing 3 includes 3 inner circles of bearing and 3 outer circles of bearing to and set up a plurality of ball between 3 inner circles of bearing and 3 outer circles of bearing, 3 outer circles of bearing and base 1 fixed connection, with rotatory tray 4 contactless, 3 inner circles of bearing and rotatory tray 4 fixed connection, with base 1 contactless.
A rotary joint 9 is arranged between the tank cover 10 and the tank opening, and the tank opening is rotated through the rotary joint 9, while the tank cover 10 is kept still.
A fixed upper cover 11 is also arranged above the tank cover 10, a plurality of stabilizers 12 are uniformly arranged on the circumference of the fixed upper cover 11, and the stabilizers 12 are connected with the air pressure tank; when the culture tank 6 rotates, the central shaft of the stabilizer 12 descends to press the tank cover 10, the rotary joint 9 rotates the culture tank, and the tank cover 10 is kept stationary. When the start culture tank 6 rotates, the center rod of the stabilizer 12 extends downward and presses the tank cover 10, so that the tank cover 10 can keep the height position unchanged and is prevented from being thrown away in the high-speed rotation process of the culture tank 6.
An upper supporting plate is further arranged above the tank cover, a plurality of stabilizers are uniformly arranged on the upper supporting plate, and the stabilizers are connected with compressed air; when the culture tank rotates, the central shaft of the stabilizer descends to press the tank cover, and the air pressure is adjustable to stabilize the rotating culture tank.
Fixed upper cover 11 horizontal connection fixes the backup pad, goes up the edge of backup pad and is provided with vertical downwardly extending's curb plate 15, and the lower extreme and the base 1 of curb plate 15 are fixed. An upper support plate and a side plate 15, which support the fixed upper cover 11, provide a support point. Meanwhile, the side plates 15 and the upper supporting plate also provide certain acting force for the fixed upper cover 11, namely, the fixed upper cover 11 is pulled during the high-speed rotation process, so that the fixed upper cover 11 is prevented from moving upwards.
The fixed upper cover 11 and the tank cover 10 are provided with a plurality of connecting holes, the connecting holes of the fixed upper cover 11 and the connecting holes of the tank cover 10 are coaxial in sequence, and a manual bolt 14 penetrates through each connecting hole. The can lid 10 is secured by a manual latch 14 to prevent rotation of the can lid 10. The perfusion system, gas exchange system, stirrer shaft 8 and suction system that are realized to penetrate through the tank cover 10 into the culture tank 6 can all be kept stationary.
Example six:
the present embodiment further designs a perfusion system, which comprises a flow guide tube 7 and a culture medium renewal system connected with the liquid inlet pipe, wherein the flow guide tube 7 is vertically inserted from the tank cover 10 to be close to the bottom of the culture tank 6. Old culture medium in the culture tank 6 is sucked out through the draft tube 7, and then fresh culture medium is conveyed into the culture tank 6 through the liquid inlet pipe.
Example seven:
in this embodiment, a flow gathering groove is further designed, and as can be seen from fig. 3, the bottom of the culture tank 6 is provided with a circle of flow gathering groove 20 concentric with the bottom, the periphery of the bottom of the culture tank 6 faces the flow gathering groove 20, and the bottom of the culture tank 6 is provided with a slope inclining downwards towards the flow gathering groove 20 from the center of the culture tank 6; the lower end of the draft tube 7 is inserted into the flow collecting groove 20 and suspended therein. When the components in the culture tank 6 need to be completely sucked, the culture medium only stored at the bottom of the culture tank 6 flows towards the collecting groove 20 under the action of the slope, so that the culture medium at the bottom of the culture tank 6 can be more fully sucked out. The distance between the guide pipe 7 and the stirring shaft 8 is larger than the width of the stirring blade 5, so that the stirring blade 5 is ensured not to touch the guide pipe 7 when rotating.
Example eight:
in the embodiment, a ventilation system 13 is further designed, wherein the ventilation system 13 comprises a gas supply end valve, a flow meter, a filter and a ventilation pipeline which is positioned in the axial direction and penetrates through the stirring shaft, and the tail end of the ventilation pipeline is provided with a micro-bubble generator; and the upper end of the vent pipe is provided with an air inlet passage connected with the vent pipe. The microbubble generation disperses the gas entering the culture tank 6 into smaller, more uniform gas particles, increasing the speed and uniformity of gas dispersion into the culture medium.
Example nine:
as can be seen in connection with fig. 1, rollers 16 are arranged below the incubator, the rollers 16 being provided with brake pads. Every corner of the bottom of thermostated container all sets up a gyro wheel 16, is convenient for promote the thermostated container to remove, after pushing away suitable position, presses the brake block, and gyro wheel 16 is locked, can not remove, and the thermostated container can fix a position in fixed position. The roller 16 can be universal wheels, and the pushing and turning are more flexible and convenient.
The technical means disclosed by the scheme of the utility model is not limited to the technical means disclosed by the technical means, but also comprises the technical scheme formed by the arbitrary combination of the technical characteristics.
In light of the foregoing, it will be apparent to those skilled in the art from this disclosure that various changes and modifications can be made without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. A vertical rotary tank bioreactor for culturing cells in a large scale comprises a rotatable culture tank, wherein a tank cover is arranged at the tank opening of the culture tank in a sealing manner; the culture tank is characterized in that a rotary driving device is arranged below the bottom of the culture tank and can drive the culture tank to rotate along the vertical central axis of the culture tank; cultivate the fixed irrotational of jar cover, it has a plurality of interface to open on the cover, be provided with rotary joint between cover and the jar mouth, rotary joint realizes the airtight rotation of culture tank.
2. The vertical rotary tank bioreactor for large-scale cell culture according to claim 1, wherein the interface on the tank cover is further connected with different kinds of electrodes, an inlet/outlet pipeline, an online cell detection system, a stirring system, a perfusion system and an aeration system;
the bioreactor is arranged in a thermostat, an observation window is arranged on a door of the thermostat, a partition plate or glass made of transparent materials is arranged in the observation window, and the thermostat is further provided with a constant temperature system and a temperature display system.
3. The vertical rotary-tank bioreactor for large-scale cell culture according to claim 2, wherein the bottom of the culture tank is provided with a criss-cross groove, and the intersection point of the criss-cross grooves is located at the center of the bottom of the tank;
the rotary driving device comprises a rotary tray and a bottom motor, the upper surface of the rotary tray is provided with a cross convex strip in a cross shape, the intersection point of the cross convex strip is located at the center of the rotary tray, and a cross groove at the lower part of the rotary tank body is arranged on the cross convex strip of the rotary tray and is just inserted into the cross groove in an inosculation manner.
4. The vertical rotary tank bioreactor for mass cultivation of cells according to claim 3, wherein the rotary tray is provided at its center with a downwardly extending connection sleeve connected to a driving shaft of a bottom motor;
the bottom motor is arranged in the base, a bearing is arranged between the rotary tray and the base, the rotary tray is driven by the bottom motor to synchronously rotate with the culture tank, and the base is kept still.
5. The vertical rotary tank bioreactor for large-scale cell culture according to claim 2, wherein an upper support plate is further arranged above the tank cover, a plurality of stabilizers are uniformly arranged on the upper support plate, and the stabilizers are connected with compressed air; when the culture tank rotates, the central shaft of the stabilizer descends to press the tank cover, and the air pressure is adjustable to stabilize the rotating culture tank.
6. The vertical rotary tank bioreactor for large-scale cell culture according to claim 5, wherein the upper support plate is connected to the upper end of the side plate by screws, and the lower end of the side plate is fixed to the base;
the cover has been seted up a plurality of connecting hole, it is equipped with the bolt to go up the backup pad, and the bolt inserts in the cover connecting hole in order to prevent the cover rotatory.
7. The vertical rotary-tank bioreactor for mass cultivation of cells according to any one of claims 2 to 6, wherein the bottom of the culture tank is provided with a circle of concentric collection grooves, a guide pipe is provided 2 to 3mm above the grooves and is installed on the cover, the bottom of the culture tank is provided with a downward slope from the periphery toward the collection grooves and from the center toward the collection grooves.
8. The vertical rotary tank bioreactor for large-scale cell culture according to claim 7, wherein the aeration system comprises a gas supply end valve, a flow meter, a filter and an aeration pipe axially penetrating in the stirring shaft, and the end of the aeration pipe is provided with a microbubble generator; and the upper end of the vent pipe is provided with an air inlet passage connected with the vent pipe.
9. The vertical rotary tank bioreactor for large-scale cell culture according to claim 1, wherein the culture tank is cylindrical, the top of the culture tank is shoulder-shaped and closes to form a necking tank opening, a stirring shaft is arranged in the culture tank and located on the axial center of the culture tank, a stirring paddle is arranged at the position of the stirring shaft close to the lower end of the stirring shaft, and a top motor is connected to the top end of the stirring shaft and drives the stirring shaft to rotate.
10. The vertical rotary tank bioreactor for mass cultivation of cells according to claim 2, wherein rollers provided with brake plates are provided under the incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920581654.6U CN210163462U (en) | 2019-04-26 | 2019-04-26 | Vertical rotary tank bioreactor for large-scale cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920581654.6U CN210163462U (en) | 2019-04-26 | 2019-04-26 | Vertical rotary tank bioreactor for large-scale cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210163462U true CN210163462U (en) | 2020-03-20 |
Family
ID=69790635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920581654.6U Active CN210163462U (en) | 2019-04-26 | 2019-04-26 | Vertical rotary tank bioreactor for large-scale cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210163462U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110016437A (en) * | 2019-04-26 | 2019-07-16 | 南京比瑞生物科技有限公司 | Vertical rotating tank bioreactor for large-scale culture cell |
-
2019
- 2019-04-26 CN CN201920581654.6U patent/CN210163462U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110016437A (en) * | 2019-04-26 | 2019-07-16 | 南京比瑞生物科技有限公司 | Vertical rotating tank bioreactor for large-scale culture cell |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106701563B (en) | Automatic Control multifunctional solid fermentor | |
| RU2538170C1 (en) | Vortex bioreactor | |
| CN115369026B (en) | A fully automatic and fully enclosed cell preparation workstation and cell processing method | |
| CN102199537A (en) | Membrane bioreactor used in microgravity environment and simulated microgravity environment | |
| CN210163462U (en) | Vertical rotary tank bioreactor for large-scale cell culture | |
| CN207828283U (en) | A kind of biological fermentation tank with highly effective oxygen dissolving effect | |
| CN112080406A (en) | Stem cell separation device | |
| CN208898901U (en) | Fixed bed and suspension dual-purpose bioreactors | |
| CN208038422U (en) | A kind of Yoghourt fermentation system | |
| CN207793239U (en) | A kind of stem cell separator | |
| AU2021100145A4 (en) | Large-scale culture method of helicobacter pylori | |
| CN205903823U (en) | Tealeaves rehydration mixer | |
| CN110396469B (en) | Microalgae fixed flue gas CO 2 Device and process for preparing biodiesel | |
| CN208395183U (en) | A kind of vertical type solid fermentor | |
| CN208701056U (en) | Degerming fermentor | |
| CN211595675U (en) | Online ultrasonic stripping cell disruption bioreactor | |
| RU2355751C1 (en) | Vortex reactor for carrying out biotechnological processes under microgravity conditions | |
| RU2763318C1 (en) | Laboratory multi-platform gas vortex bioreactor | |
| RU2363729C1 (en) | Apparatus for suspension cultivation of tissue or microorganism cells | |
| CN110791431B (en) | Online ultrasonic stripping broken cell bioreactor and method for harvesting viruses | |
| CN110016437A (en) | Vertical rotating tank bioreactor for large-scale culture cell | |
| CN109988700A (en) | A kind of stem cell separator | |
| CN210001865U (en) | kinds of gene stem cell extraction equipment | |
| CN207828275U (en) | A kind of biological fermentation tank of uniform distribution | |
| CN208949309U (en) | A kind of automatic cytological culture apparatus for simulating organismic internal environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |