CN210090369U - A multi-channel surface acoustic wave biosensor detection fixture and system - Google Patents

Abstract

The utility model provides a multichannel surface acoustic wave biosensor detects anchor clamps and system. The clamp comprises a clamp body and a sealing layer, wherein the clamp body can be opened and closed, the clamp body comprises a first clamp body and a second clamp body, and the sealing layer is arranged between the first clamp body and the second clamp body; an acoustic surface wave chip placing area is arranged on the second clamp body, and the acoustic surface wave chip comprises a plurality of sensing parts; the sealing layer comprises a plurality of through holes which correspond to the positions and the shapes of the sensing parts one by one; when the clamp is closed, a plurality of closed detection pools corresponding to the sensing parts are formed, a plurality of flow passages communicated with the sensing parts correspondingly are arranged above the detection pools, and each flow passage is provided with a liquid inlet pipeline and a liquid outlet pipeline which penetrate through the first clamp body. When the clamp is closed, a plurality of closed detection pools are formed, the liquid to be detected and substances on the surface of the sensing part are subjected to biochemical reaction in the closed detection pools, the influence of liquid leakage and evaporation on the measurement precision is avoided, and multi-channel independent detection is realized.

Description

A multi-channel surface acoustic wave biosensor detection fixture and system

technical field

The utility model relates to a detection fixture, in particular to a multi-channel surface acoustic wave biological sensor detection fixture and a system.

Background technique

The surface acoustic wave biosensor is a new type of acoustic sensor applied to the detection of liquid biomolecules. The biochemical reaction between the biomolecules and the substances on the sensing surface of the surface acoustic wave chip will change the signal of the surface acoustic wave on the surface acoustic wave chip. For example, after the biochemical reaction, the weight of the surface acoustic wave sensing surface increases, which will change the frequency of the surface acoustic wave on it. Therefore, by detecting the change of the surface acoustic wave signal parameters on the surface acoustic wave chip, the treatment Qualitative and quantitative analysis of analytes.

Currently developed surface acoustic wave biosensors include glucose surface acoustic wave sensors, DNA determination surface wave sensors and tumor marker surface wave sensors. The most important devices are usually exposed to the air. The humidity of the environment itself, air flow, dust, etc. will affect the signal of the chip, and the method of adding liquid is manual drip coating, which greatly reduces the authenticity and reliability of the data. Due to the physical properties of the liquid, when the liquid to be measured is introduced into the surface wave chip sensing surface for biochemical reactions, it is prone to leakage and evaporation, which introduces great errors to the measurement results and affects the measurement accuracy.

The Chinese patent with the publication number CN207964727U in the prior art discloses a surface acoustic wave biosensor clamp, although when the clamp of the patent is closed, the liquid inlet pipeline, the inner cavity of the silicone sealing ring and the liquid outlet pipeline form a liquid passage. , the liquid to be measured biochemically reacts with the substances on the sensing surface of the surface acoustic wave chip in the inner cavity of the silicone seal, avoiding the influence of liquid leakage and evaporation on the measurement accuracy. The structure relies on the attractive force of the magnet, There may be a phenomenon where the closure is not tight and the liquid leaks. In this patent, the input and output of the liquid in the inner cavity of the sealing ring is realized through the micro flow channel, which increases the difficulty of fixture processing. In addition, this patent can only complete single-channel measurement, and cannot complete multi-channel measurement.

Utility model content

In order to overcome the above-mentioned defects in the prior art, the purpose of the present invention is to provide a multi-channel surface acoustic wave biosensor detection fixture and system.

In order to achieve the above purpose of the present utility model, according to the first aspect of the present utility model, the present utility model provides a multi-channel surface acoustic wave biosensor detection fixture, which includes an openable and closable fixture body and a sealing layer. The clamp body includes a first clamp body and a second clamp body, and the sealing layer is arranged between the first clamp body and the second clamp body;

A surface acoustic wave chip placement area is provided on the second fixture body, and the surface acoustic wave chip includes a plurality of sensing parts; the sealing layer includes a plurality of through holes corresponding to the positions and shapes of the sensing parts. ;

When the fixture body is closed, the first fixture body, the sealing layer, the surface acoustic wave chip and the second fixture body are in close contact in order from top to bottom to form a plurality of closed detection cells corresponding to the sensing parts. The top of the pool is provided with a plurality of flow channels corresponding to the sensing part, and each flow channel has a liquid inlet pipeline and a liquid outlet pipeline running through the body of the first fixture, the liquid inlet pipeline outlet and the liquid outlet pipe The pipeline input ports are respectively connected with the through holes, and the liquid inlet pipeline and the liquid outlet pipeline are connected with the external pipeline on the surface of the first fixture body;

It also includes an input electrode electrically connected to the input end of the surface acoustic wave chip, and an output electrode electrically connected to the output end of the surface acoustic wave chip.

The beneficial effects of the above technical solutions are: the first clamp body and the second clamp body can be opened and closed, which is convenient for assembly; when the clamps are closed, a plurality of closed detection pools are formed, each detection pool includes a sensing part, and the detection pool The liquid channel to be measured is formed with the flow channel corresponding to its internal sensing part, and the liquid to be measured undergoes a biochemical reaction with the substances on the surface of the sensing part in the closed detection tank, avoiding leakage and evaporation of the liquid and affecting the measurement accuracy. It realizes multi-channel detection, and each channel is isolated from each other by the sealing layer body, which improves the detection efficiency and accuracy; the input electrode is used to input electrical signals to the surface acoustic wave chip, and the output electrode transmits the signal of the surface acoustic wave chip. The output signal is convenient for the connection between the surface acoustic wave chip and external instrumentation equipment.

In a preferred embodiment of the present invention, the liquid inlet pipeline and the liquid outlet pipeline penetrate through the first clamp body in the vertical direction.

The beneficial effects of the above technical solutions are: easy processing, the shortest length of the liquid inlet pipeline and the liquid outlet pipeline, the reduction of the liquid circulation path to be measured, and the improvement of the detection efficiency.

In a preferred embodiment of the present invention, it further comprises a positioning mechanism for aligning the through holes of the sealing layer with the sensing parts of the surface acoustic wave chip one by one;

And/or also includes a clamping mechanism for ensuring that the first clamp body, the sealing layer, the surface acoustic wave chip and the second clamp body are in close contact in sequence.

The beneficial effects of the above technical solutions are as follows: the through hole of the sealing layer is made to correspond to the installation position of the sensing part of the surface acoustic wave chip through the positioning mechanism, so as to avoid offset; the clamping mechanism can improve the airtightness of the detection cell and avoid the leakage of the liquid to be tested. .

In a preferred embodiment of the present invention, the positioning mechanism includes an alignment post respectively disposed on the pressing surfaces of the first clamp body and the second clamp body and a concave hole matched with the alignment post;

And/or the clamping mechanism includes a clamping hole provided on the first clamp body, a threaded hole provided on the second clamp body corresponding to the position of the clamping hole, and a thread passing through the clamping hole and the threaded hole. Attachment screws.

The beneficial effects of the above technical solutions are as follows: a simple structure of the positioning mechanism with high positioning accuracy is disclosed; and a simple structure of the clamping mechanism with good clamping effect is disclosed.

In a preferred embodiment of the present invention, the surface acoustic wave chip includes interdigital electrodes located on both sides, chip connection ends located at both ends of the interdigital electrodes, and a longitudinal arrangement disposed between the two interdigitated electrodes. the first sensing part, the second sensing part and the third sensing part;

The chip connection end of one of the interdigitated electrodes is used as the input end of the surface acoustic wave chip, and the chip connection end of the other interdigitated electrode is used as the output end of the surface acoustic wave chip;

The sealing layer includes longitudinally arranged first through holes, second through holes and third through holes corresponding to the first sensing portion, the second sensing portion and the third sensing portion, respectively.

The beneficial effects of the above-mentioned technical solutions are as follows: simultaneous three-channel independent testing is realized.

In a preferred embodiment of the present invention, the input electrode and the output electrode are arranged on the upper surface of the first clamp body and have connection ends respectively on the lower surface of the first clamp body. After the clamp body is pressed together, the input electrode is The connection end of the electrode is connected with the input end of the surface acoustic wave chip, and the connection end of the output electrode is connected with the output end of the surface acoustic wave chip.

The beneficial effects of the above technical solutions are: the input electrode and the output electrode are respectively arranged on the first clamp body, and when the clamp is pressed together, the connection end of the input electrode and the output electrode will exert pressure on the surface acoustic wave chip to ensure the biochemical reaction. During the process, the stability of the chip position reduces the impact of vibration on the measurement. The other end of the input electrode and the other end of the output electrode respectively pass through the outer surface of the first fixture body, which is convenient for connection with external equipment. it is good.

In a preferred embodiment of the present invention, it further includes an input circuit board and an output circuit board fixed on the upper surface of the first fixture body;

An input signal connector is welded on the input circuit board, and a first pogo pin penetrating the first clamp body is in contact and electrically connected with the input end of the surface acoustic wave chip when the clamp body is pressed together;

An output signal connector is welded on the input circuit board, and a second pogo pin penetrating the first clamp body is in contact and electrically connected to the output end of the surface acoustic wave chip when the clamp body is pressed together.

The beneficial effects of the above technical solutions are: due to the existing equipment such as a signal generator or a signal processing device, such as a frequency meter, its output port generally adopts various standard signal connector interfaces. Matching, set the input signal signal connector and the output signal connector to ensure that the distortion is small during the signal transmission process, the anti-interference ability is strong, the effective connection is achieved, and the connection loss is reduced. The connection between the input electrode and the input signal connector, and the connection between the output electrode and the output signal connector are realized through the input circuit board and the output circuit board, which standardizes the connection and avoids the messiness, poor contact, and easy damage caused by the direct connection of the wires. ; Fix the input circuit board and the output circuit board on the upper surface of the first fixture body, so that the electrical connection is not easy to be disconnected and damaged, and provides support and fixation for the input signal connector and the output signal connector, making it easier to connect operate.

In a preferred embodiment of the present invention, it also includes at least one first liquid connection head and at least one second liquid connection head;

The first liquid connection head is connected with the input port of the liquid inlet pipeline; the second liquid connection head is connected with the output port of the liquid outlet pipeline.

The beneficial effects of the above technical solutions are: the tightness of the liquid pipeline is increased through the first liquid connecting head and the second liquid connecting head, and the leakage of the liquid pipeline at the input port of the liquid inlet pipeline and the output port of the liquid outlet pipeline is avoided. .

In a preferred embodiment of the present invention, the surface acoustic wave chip placement area is a chip placement slot opened on the second fixture body;

Alternatively, grooves are provided symmetrically in the lateral or longitudinal direction of the chip placement groove.

The beneficial effects of the above technical solutions are: the chip placement groove makes the surface acoustic wave chip place stably, and also has a positioning function to ensure that the chip sensing part is located in the through hole of the sealing layer after the clamp is pressed; the groove is convenient to take on the chip placement groove. SAW chip.

In order to achieve the above purpose of the present utility model, according to the second aspect of the present utility model, the present utility model provides a surface acoustic wave biosensor detection system, comprising the multi-channel surface acoustic wave biosensor detection fixture of the present utility model , and each detection pool is provided with a liquid pool to be tested, a driving pump, a signal generator and a signal processing device;

The outlet of the liquid pool to be tested is connected with the pipeline of the input end of the driving pump, and the output end of the driving pump is connected with the input port of the liquid inlet pipeline of the corresponding detection tank or the pipeline of the first liquid connection head, and the liquid outlet pipeline of the corresponding detection tank is output. The port or the second liquid connector is connected with the inlet pipeline of the liquid pool to be tested;

The input electrode or the input signal connector corresponding to the detection cell is electrically connected to the output end of the signal generator respectively, and the output electrode or the output signal connector corresponding to the detection cell is electrically connected to the input end of the signal processing device respectively;

The signal processing device is a frequency meter or a network analyzer.

The beneficial effects of the above technical solutions are: the system can realize multi-channel testing of any one or more liquids, has versatility, avoids the influence of liquid leakage and evaporation on the measurement accuracy, has small response distortion, and improves the measurement accuracy. By driving the pump, the liquid to be tested can automatically flow into the liquid inlet pipeline, the detection tank and the liquid outlet pipeline. The output signal is processed by the signal processing device to obtain the test result.

Additional aspects and advantages of the invention will be set forth, in part, from the following description, and in part will be apparent from the following description, or learned by practice of the invention.

Description of drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily understood from the following description of embodiments in conjunction with the accompanying drawings, wherein:

1 is a schematic diagram of a partial structure assembly of a multi-channel surface acoustic wave biosensor detection fixture of a specific embodiment of the present invention;

2 is a schematic diagram of the overall assembly of a multi-channel surface acoustic wave biosensor detection fixture of a specific embodiment of the present invention;

3 is an assembly schematic diagram of a second fixture body and a surface acoustic wave chip of a multi-channel surface acoustic wave biosensor detection fixture of a specific embodiment of the present invention;

4 is a top view of a multi-channel surface acoustic wave biosensor detection fixture of a specific embodiment of the present invention;

FIG. 5 is a system block diagram of a surface acoustic wave biosensor detection system in a specific embodiment of the present invention.

Reference number:

1 Fixture body; 11 First fixture body; 111 Liquid inlet pipe; 112 Liquid outlet pipe; 113 Clamping hole; 114 Fixing screw; 115 Recessed hole; 116 Input electrode connection end; 117 Output electrode connection end; 121 chip placement slot; 122 alignment post; 123 groove; 124 threaded hole; 2 sealing layer; 21 through hole; 3 surface acoustic wave chip; 31 sensing part; 32 chip connection end.

Detailed ways

The following describes in detail the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, but should not be construed as a limitation of the present invention.

In the description of the present invention, unless otherwise specified and limited, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a mechanical connection or an electrical connection, or it may be a The internal communication between the two elements may be directly connected or indirectly connected through an intermediate medium, and those of ordinary skill in the art can understand the specific meanings of the above terms according to specific circumstances.

In a preferred embodiment of the present invention, FIG. 1 to FIG. 4 are schematic diagrams of assembly in one embodiment of the present invention. In this embodiment, the clamp includes a clamp body 1 and a sealing layer 2 that can be opened and closed. , the fixture body 1 includes a first fixture body 11 and a second fixture body 12, and the sealing layer 2 is arranged between the first fixture body 11 and the second fixture body 12;

The second fixture body 12 is provided with a placement area for the surface acoustic wave chip 3 , the surface acoustic wave chip 3 includes a plurality of sensing parts 31 ; the sealing layer 2 includes a plurality of through holes corresponding to the positions and shapes of the sensing parts 31 one-to-one twenty one;

When the fixture body 1 is closed, the first fixture body 11 , the sealing layer 2 , the surface acoustic wave chip 3 and the second fixture body 12 are in close contact in order from top to bottom to form a plurality of closed detections corresponding to the sensing portion 31 . The upper part of the detection tank is provided with a plurality of flow channels corresponding to the sensing part 31, and each flow channel has a liquid inlet pipeline 111 and a liquid outlet pipeline 112 running through the first fixture body 11. The liquid inlet pipeline The output port 111 and the input port of the liquid outlet pipeline 112 are respectively connected with the through hole 21, and the liquid inlet pipeline 111 and the liquid outlet pipeline 112 are connected to the external pipeline on the surface of the first fixture body 11;

It also includes an input electrode electrically connected to the input end of the surface acoustic wave chip 3 , and an output electrode electrically connected to the output end of the surface acoustic wave chip 3 .

In this embodiment, the first clamp body 11 and the second clamp body 12 are preferably but not limited to bolt connection, shaft connection, snap connection and other common detachable connection forms in the art, and their material is preferably but not limited to plexiglass. The material of the sealing layer 2 is preferably, but not limited to, polydimethylsiloxane (PDMS, polydimethylsiloxane), which is a type of organic silicon with low cost, simple use, and good chemical inertness. The sealing layer 2 is preferably, but not limited to, a rectangular, circular or other sheet-like structure, and a plurality of through holes 21 corresponding to the shape of the sensing portion 31 , such as square holes, circular holes, etc., are formed thereon.

In this embodiment, when the fixture is closed, the inner wall of the detection cell includes the surface of the sensing portion 31 , the inner wall of the through hole 21 of the sealing layer 2 and the portion where the pressing surface of the first fixture body 11 intersects with the through hole 21 . Relying on the gravity of the first fixture body 11 , the first fixture body 11 , the sealing layer 2 , the surface acoustic wave chip 3 and the second fixture body 12 are in close contact in order from top to bottom, so that the detection cell is sealed.

In this embodiment, in order to reduce the circulation time, the liquid inlet pipeline 111 and the liquid outlet pipeline 112 in the flow channel can be arranged in a straight line as much as possible, which is convenient for observation, and can also be arranged in parallel; the liquid inlet pipeline 111 and/or the liquid outlet pipeline The road 112 is preferably but not limited to adopt the following structure:

Structure 1: The liquid inlet pipeline 111 or the liquid outlet pipeline 112 includes a section of micro-pipeline with a smaller inner diameter. According to when the clamp is closed, a position corresponding to the lower sensing part 31 on the first clamp body 11 is opened to accommodate a single micro-pipe. The micro-through hole through which the pipe passes, the micro-pipe is passed through the micro-through hole on the first fixture body 11 and fixed with adhesive, one end of the micro-pipe enters the detection tank, and the other end is connected to an external pipeline.

Structure 2: The liquid inlet pipeline 111 or the liquid outlet pipeline 112 is a micro-pipeline directly opened on the first fixture body 11 .

In this embodiment, the number of input electrodes and output electrodes is plural, which corresponds to the number of pins of the input terminal and the output terminal of the surface acoustic wave chip 3, and may be two respectively. Preferably, the input electrode and the output electrode are arranged vertically, one end is connected to the input end or output end of the surface acoustic wave chip 3, and the other end protrudes from the upper surface of the first clamp body 11 to be connected to the external circuit, and at the same time the flow channel layer There is an electrode hole for each electrode on the top, and the input electrode and the output electrode pass through the corresponding electrode hole to connect with the input pin and output pin of the chip respectively. The connection method can be welding or contact connection. When welding, After the clamp is opened, one end of the input electrode and one end of the output electrode are welded to the chip and located on the second clamp body 12. When the clamp is pressed, the other end of the input electrode and the other end of the output electrode pass through the motor holes respectively. In the case of contact connection, after the clamp is opened, the other ends of the input electrode and the output electrode are fixed on the first clamp body 11, and as the first clamp body 11 leaves, when the clamp is pressed, one end of the input electrode and one end of the output electrode are Contact connection with chip. The input electrode and the output electrode can be made of conductors with very low impedance, or spring striker electrodes, which have low impedance, small contact area at the contact end, high strength, and retractable electrode heads.

In a preferred embodiment, the liquid inlet pipeline 111 and the liquid outlet pipeline 112 pass through the first clamp body 11 in the vertical direction.

In a preferred embodiment, a positioning mechanism for aligning the through holes 21 of the sealing layer 2 with the sensing portions 31 of the surface acoustic wave chip 3 one-to-one is also included.

In this embodiment, the positioning mechanism may be a positioning structure in which a pin is matched with a pin hole, or may be a structure in which the first clamp body 11 is inserted into the second clamp body 12 .

Preferably, the positioning is realized by the following structure: the positioning mechanism includes alignment posts 122 respectively disposed on the pressing surfaces of the first clamp body 11 and the second clamp body 12 and the concave holes 115 matched with the alignment posts 122 . The alignment posts 122 are preferably, but not limited to, cylindrical, conical or prismatic shapes, and the length of the alignment posts 122 should be less than the depth of the correspondingly matched concave holes 115 . Preferably, two pairs of correspondingly matched alignment posts 122 and concave holes 115 are provided on the diagonal vertices of the clamp body 1 .

In a preferred embodiment, it also includes a clamping mechanism for ensuring that the first clamp body 11 , the sealing layer 2 , the surface acoustic wave chip 3 and the second clamp body 12 are in close contact in sequence.

In this embodiment, the clamping mechanism may be a clamp body 12 placed on the water platform after the clamps are pressed together, and the vertical downward pneumatic head, hydraulic head or weight hammer contacts and acts on the first clamp body 12 . The upper surface of the fixture body 11 is tightly pressed.

Preferably, the clamping mechanism includes a clamping hole 113 provided on the first clamp body 11 , a threaded hole 124 provided on the second clamp body 12 corresponding to the position of the clamping hole 113 , and a threaded hole 124 passing through the clamping hole 113 The hole 124 is threaded to the fixing screw 114 . Further, two sets of clamping holes 113 , threaded holes 124 and fixing screws 114 are respectively provided on the diagonal vertices of the clamp body 1 . Further, at least one washer is arranged between the nut of the national screw and the clamping hole 113 , so that the closing force of the clamp can be adjusted at any time by means of the fixing screw 114 .

In a preferred embodiment, the surface acoustic wave chip 3 includes interdigitated electrodes located on both sides, chip connection ends 32 located at both ends of the interdigitated electrodes, and a first sensor arranged longitudinally between the two interdigitated electrodes. a sensing part, a second sensing part and a third sensing part;

The chip connection end 32 of one of the interdigitated electrodes is used as the input end of the surface acoustic wave chip 3, and the chip connection end 32 of the other interdigitated electrode is used as the output end of the surface acoustic wave chip 3;

The sealing layer 2 includes longitudinally arranged first through holes, second through holes and third through holes corresponding to the first sensing portion, the second sensing portion and the third sensing portion, respectively.

In a preferred embodiment, the input electrode and the output electrode are arranged on the upper surface of the first clamp body 11 and have connection ends on the lower surface of the first clamp body 11 respectively. After the clamp body 1 is pressed together, the connection end 116 of the input electrode It is connected to the input end of the surface acoustic wave chip 3 , and the connection end 117 of the output electrode is connected to the output end of the surface acoustic wave chip 3 .

In this embodiment, the input electrode and the output electrode pass through the electrode holes in the first fixture body 11 respectively. When the fixture is pressed together, pressure will be applied to the surface acoustic wave chip 3 to ensure that during the biochemical reaction process, the chip position stability and reduce the impact of vibration on the measurement.

In a preferred embodiment, it also includes an input circuit board and an output circuit board fixed on the upper surface of the first fixture body 11;

An input signal connector is welded on the input circuit board, and a first pogo pin that penetrates through the first fixture body 11 and is electrically connected to the input end of the surface acoustic wave chip 3 when the fixture body 1 is pressed together;

An output signal connector is soldered on the input circuit board, and a second pogo pin penetrating the first clamp body 11 is electrically connected to the output end of the surface acoustic wave chip 3 when the clamp body 1 is pressed together.

In this embodiment, the input signal connector and the output signal connector can be selected from the connectors corresponding to the interface forms commonly used by external devices such as signal generators, which can be SMA connectors, and each input electrode and output electrode are respectively connected to the SMA connector tube. The foot end is welded, and the interface end of the SMA connector is connected to the external device through a cable.

In this embodiment, the input circuit board and the output circuit board can be either printed PCB boards or flexible boards. A plurality of connection screw holes are opened on the flow channel layer for fixing the input circuit board and the output circuit board.

In a preferred embodiment, it also includes at least one first liquid connection head and at least one second liquid connection head;

The first liquid connecting head is connected with the input pipe of the liquid inlet pipe 111 ; the second liquid connecting head is connected with the output port pipe of the liquid outlet pipe 112 .

In this embodiment, the first liquid connecting head and the second liquid connecting head can be selected from PEEK joints.

In a preferred embodiment, the placement area of the surface acoustic wave chip 3 is a chip placement groove 121 opened on the second fixture body 12;

Alternatively, grooves 123 are symmetrically opened in the lateral or longitudinal direction of the chip placement groove 121 .

In this embodiment, the shape and size of the chip placement groove 121 correspond to the surface acoustic wave chip 3 . In order to ensure the installation accuracy of the surface acoustic wave chip 3 , the gap after the chip placement groove 121 is placed in the surface acoustic wave chip 3 should be small. . The depth of the groove 123 is greater than the depth of the chip placement groove 121 , which may be a waist-shaped groove.

The utility model also discloses a surface acoustic wave biosensor detection system, the system block diagram of which is shown in FIG. 5 , the system includes the multi-channel surface acoustic wave biosensor detection fixture of the present invention, and each detection cell is provided with a waiting Measuring liquid pool, driving pump, signal generator and signal processing device;

The outlet of the liquid pool to be tested is connected to the pipeline of the input end of the driving pump, the output end of the driving pump is connected to the input port of the liquid inlet pipeline 111 of the corresponding detection tank or the pipeline of the first liquid connecting head, and the output of the liquid outlet pipeline 112 of the corresponding detection tank is output. The port or the second liquid connector is connected with the inlet pipeline of the liquid pool to be tested;

The input electrode or the input signal connector corresponding to the detection cell is electrically connected to the output end of the signal generator respectively, and the output electrode or the output signal connector corresponding to the detection cell is electrically connected to the input end of the signal processing device respectively;

The signal processing device is a frequency meter or a network analyzer.

In this embodiment, the system can realize the simultaneous testing of multiple liquids to be tested, which is versatile, can avoid the influence of liquid leakage and evaporation on the measurement accuracy, and has small response distortion, which improves the measurement accuracy. By driving the pump, the liquid to be tested can automatically flow into the liquid inlet pipeline 111 , the inner cavity of the through hole 21 of the sealing layer 2 , and flow out from the liquid outlet pipeline 112 . The output signal is processed by the signal processing device to obtain the test result. When the signal generator has multiple output interfaces and the signal processing device has multiple input interfaces, the signal generator and the signal processing device can be shared by multiple detection cells.

In the description of this specification, description with reference to the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples", etc., mean specific features described in connection with the embodiment or example , structure, material or feature is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention. Variations, the scope of the present invention is defined by the claims and their equivalents.

Claims (10)

1. The multi-channel surface acoustic wave biosensor detection clamp is characterized by comprising a clamp body (1) and a sealing layer (2), wherein the clamp body (1) can be opened and closed, the clamp body (1) comprises a first clamp body (11) and a second clamp body (12), and the sealing layer (2) is arranged between the first clamp body (11) and the second clamp body (12);

a surface acoustic wave chip placing area is arranged on the second clamp body (12), and the surface acoustic wave chip (3) comprises a plurality of sensing parts (31); the sealing layer (2) comprises a plurality of through holes (21) corresponding to the positions and the shapes of the sensing parts (31) in a one-to-one mode;

when the clamp body (1) is closed, the first clamp body (11), the sealing layer (2), the surface acoustic wave chip (3) and the second clamp body (12) are in close contact with one another in sequence from top to bottom to form a plurality of closed detection pools corresponding to the sensing part (31), a plurality of flow channels correspondingly communicated with the sensing part (31) are arranged above the detection pools, each flow channel is provided with a liquid inlet pipeline (111) and a liquid outlet pipeline (112) penetrating through the first clamp body (11), an output port of the liquid inlet pipeline (111) and an input port of the liquid outlet pipeline (112) are respectively connected with the through hole (21), and the liquid inlet pipeline (111) and the liquid outlet pipeline (112) are connected with an external pipeline on the surface of the first clamp body (11);

the surface acoustic wave chip also comprises an input electrode electrically connected with the input end of the surface acoustic wave chip (3) and an output electrode electrically connected with the output end of the surface acoustic wave chip (3).

2. The multi-channel surface acoustic wave biosensor test fixture of claim 1, wherein said inlet pipe (111) and outlet pipe (112) extend through the first fixture body (11) in a vertical direction.

3. The multi-channel surface acoustic wave biosensor detecting jig according to claim 1, further comprising a positioning mechanism that aligns the through holes (21) of the sealing layer (2) one by one with the sensing portions (31) of the surface acoustic wave chip (3);

and/or the surface acoustic wave chip packaging structure further comprises a clamping mechanism for ensuring that the first clamp body (11), the sealing layer (2), the surface acoustic wave chip (3) and the second clamp body (12) are in close contact in sequence.

4. The multi-channel surface acoustic wave biosensor detecting clamp of claim 3, wherein the positioning mechanism includes a registration post (122) and a concave hole (115) matched with the registration post (122) respectively arranged on the pressing surface of the first clamp body (11) and the second clamp body (12);

and/or the clamping mechanism comprises a clamping hole (113) arranged on the first clamp body (11), a threaded hole (124) arranged on the second clamp body (12) and corresponding to the clamping hole (113), and a fixing screw (114) penetrating through the clamping hole (113) and in threaded connection with the threaded hole (124).

5. The multi-channel surface acoustic wave biosensor detecting jig according to claim 1, wherein the surface acoustic wave chip (3) includes interdigital electrodes on both sides, a chip connection terminal (32) at both ends of the interdigital electrodes, and a first sensing portion, a second sensing portion, and a third sensing portion arranged longitudinally in the middle of the two interdigital electrodes;

the chip connecting end (32) of one interdigital electrode is used as the input end of the surface acoustic wave chip (3), and the chip connecting end (32) of the other interdigital electrode is used as the output end of the surface acoustic wave chip (3);

the sealing layer (2) comprises a first through hole, a second through hole and a third through hole which are longitudinally arranged and respectively correspond to the first sensing part, the second sensing part and the third sensing part.

6. The multi-channel SAW biosensor detection clamp of claim 1, wherein said input electrode and output electrode are disposed on the upper surface of the first clamp body (11) and have connection terminals on the lower surface of the first clamp body (11), respectively, when the clamp body (1) is pressed, the connection terminal (116) of said input electrode is connected to the input terminal of the SAW chip (3), and the connection terminal (117) of said output electrode is connected to the output terminal of the SAW chip.

7. The multi-channel surface acoustic wave biosensor detecting jig of claim 6, further comprising an input circuit board and an output circuit board fixedly provided on an upper surface of the first jig body (11);

the input circuit board is welded with an input signal connector and a first spring pin which penetrates through the first clamp body (11) and is in contact and electric connection with the input end of the surface acoustic wave chip (3) when the clamp body (1) is pressed;

the input circuit board is welded with an output signal connector and a second spring pin which penetrates through the first clamp body (11) and is in contact and electric connection with the output end of the surface acoustic wave chip (3) when the clamp body (1) is pressed.

8. The multi-channel surface acoustic wave biosensor detecting fixture of claim 1, further comprising at least one first liquid connector and at least one second liquid connector;

the first liquid connector is connected with an input port pipeline of the liquid inlet pipeline (111); the second liquid connector is connected with an output port pipeline of the liquid outlet pipeline (112).

9. The multi-channel surface acoustic wave biosensor detecting jig of claim 1, wherein the surface acoustic wave chip placement region is a chip placement groove (121) opened on the second jig body (12);

or grooves (123) are symmetrically formed in the transverse direction or the longitudinal direction of the chip placing groove (121).

10. A surface acoustic wave biosensor detecting system, comprising the multi-channel surface acoustic wave biosensor detecting jig of any one of claims 1 to 9, provided with a liquid pool to be detected, a drive pump, a signal generator and a signal processing device for each detecting pool;

the outlet of the liquid pool to be detected is connected with the input end pipeline of the driving pump, the output end of the driving pump is connected with the input port of the liquid inlet pipeline (111) or the pipeline of the first liquid connector of the corresponding detection pool, and the output port of the liquid outlet pipeline (112) or the second liquid connector of the corresponding detection pool is connected with the inlet pipeline of the liquid pool to be detected;

the input electrode or the input signal connector corresponding to the detection cell is respectively and electrically connected with the output end of the signal generator, and the output electrode or the output signal connector corresponding to the detection cell is respectively and electrically connected with the input end of the signal processing device;

the signal processing device is a frequency meter or a network analyzer.

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