CN210012839U - A constant temperature shaking incubation device suitable for pipetting workstations - Google Patents

Abstract

The utility model discloses a constant temperature oscillation incubation device suitable for a liquid transfer workstation, which comprises a constant temperature oscillation mechanism and an auxiliary mechanism; the constant-temperature oscillating mechanism comprises an oscillating module and a constant-temperature module, wherein the oscillating module comprises a fixed base, a linear motor, a motor shaft, a first fixed table, a second fixed table, a middle sliding table, an upper sliding table, an eccentric bearing, an upper sliding rod, a lower sliding rod, a self-lubricating sleeve and an oscillating table; the constant temperature module is arranged above the oscillation module and can be used for placing a microporous plate, the auxiliary mechanism comprises a box body, and a microporous plate support is placed on the box body. The device drives the eccentric bearing to rotate through the linear motor, and the eccentric bearing drives the sliding table to reciprocate along the sliding rod, so that the oscillating table is driven to realize an oscillating function. The device can cooperate mechanical tongs and the multichannel pipettor that move the liquid workstation, realizes that the micropore board is at the constant temperature oscillation and incubate the automatic transfer between device and the liquid workstation, realizes full-automatic high flux biological sample constant temperature incubation operation simultaneously.

Description

A constant temperature shaking incubation device suitable for pipetting workstations

technical field

The utility model relates to a constant temperature vibration incubation device, in particular to a constant temperature vibration incubation device suitable for a liquid transfer workstation.

Background technique

Constant-temperature shaking incubation is a commonly used operation in biochemical experiments, and is often used in experiments such as probe modification and nucleic acid hybridization. Most of the common constant temperature oscillation devices are incubators with large specifications. They have a single structure and function and cannot be integrated into the automatic pipetting workstation. At the same time, the access of consumables depends on manual operation, which cannot be matched with the mechanical gripper of the pipetting workstation. And multi-channel pipette, realize automatic transfer of microplate between constant temperature shaking incubation device and pipetting workstation, which is not conducive to the realization of laboratory automation.

Utility model content

Purpose of the utility model: The purpose of the utility model is to provide a constant temperature oscillation incubation device, which is suitable for various automatic pipetting workstations, and can cooperate with the mechanical gripper and multi-channel pipette of the pipetting workstation to realize fully automatic Incubation with constant temperature shaking.

Technical solution: a constant temperature oscillation incubation device suitable for a liquid transfer workstation, comprising a constant temperature oscillation mechanism and an auxiliary mechanism; the constant temperature oscillation mechanism includes an oscillation module and a constant temperature module, and the oscillation module includes a fixed base with a groove in the middle, The linear motor is fixedly connected with the fixed base, the motor shaft is connected with the linear motor, the eccentric bearing is fixed on the motor shaft, the first fixed platform and the second fixed platform are fixed on the fixed base, and the sliding rod passing through the middle sliding platform is fixed on the first fixed platform. On the fixed table and the second fixed table, the upper sliding rod passing through the upper sliding table is fixed on the side wall of the fixed base. The upper sliding rod and the lower sliding rod are perpendicular to each other. The eccentric bearing passes through the hollow of the upper sliding table and is flush with the top of the upper sliding table. The protrusion on the upper end of the eccentric bearing matches the groove in the middle of the oscillating table, and the oscillating table is fixedly connected with the upper sliding table; The microplate is placed; the auxiliary mechanism includes a box body, the inside of the box body is a hollow structure, a microplate support is placed on the top of the box body, and the box body is connected with the constant temperature oscillation mechanism.

Beneficial effects: (1) The utility model has complete functions, and the whole device can be directly integrated into the automatic liquid pipetting workstation. (2) The utility model can fully realize the oscillation function required for the test under the condition of simple and compact structure, and the oscillation module and the constant temperature module can work independently without interfering with each other, and can also work simultaneously. (3) The utility model can cooperate with the mechanical gripper and the multi-channel pipette of the liquid transfer workstation to realize the automatic transfer of the microplate between the constant temperature oscillation incubation device and the liquid transfer workstation. (4) The utility model has an independent control unit, which can be used as an independent miniaturized device, and can also be easily integrated into large-scale biochemical analysis instruments and other devices to meet various actual needs of users.

Description of drawings

Fig. 1 is the structural representation of the present utility model;

Fig. 2 is the top sectional view of the constant temperature oscillation mechanism of the present utility model;

Fig. 3 is the front sectional view of the constant temperature oscillation mechanism of the present utility model;

Fig. 4 is the overall outline drawing of the present utility model;

Fig. 5 is the structural representation of the constant temperature module of the present invention;

Figure 6 is an overall outline view of the utility model added to the microplate.

Detailed ways

As shown in FIGS. 1 and 4 , the constant temperature oscillation incubation device of the present invention includes a constant temperature oscillation mechanism and an auxiliary mechanism. As shown in FIGS. 2-3 , the constant temperature oscillation mechanism includes an oscillation module 101 and a constant temperature module 102 . The oscillation module 101 includes a fixed base 201 with a groove in the middle, the linear motor 202 is fixedly connected with the fixed base 201, the motor shaft 203 is connected with the linear motor 202, the eccentric bearing 208 is fixed on the motor shaft, the first fixed platform 204 is connected to the second fixed base 204 The fixed table 205 is fixed on the fixed base, the middle sliding table 206 and the upper sliding table 207 are both in the shape of a few glyphs and the middle is hollowed out, two upper sliding rods 209 pass through the upper sliding table 207, and four side walls of the middle sliding table 206 can be provided with Two self-lubricating sleeves 213, two upper sliding rods 209 pass through the self-lubricating sleeve, two sliding rods 210 pass through the self-lubricating sleeve 213 and are respectively fixedly connected to the first fixing table 204 and the second fixing table 205, and two The sliding rods 210 and the two upper sliding rods 209 are perpendicular to each other and are arranged in a well-shaped pattern. The eccentric bearing 208 passes through the hollow of the upper sliding table 207, and is flush with the top of the upper sliding table 207. The eccentric bearing is fixedly connected with the oscillation table, specifically, the two cylindrical protrusions on the upper end of the eccentric bearing 208 and the middle of the oscillation table 214. The two cylindrical grooves are matched, and the oscillating table 214 and the upper sliding table 207 are fixedly connected by four screws. The oscillation module further includes shock-absorbing columns 211. There are 6 shock-absorbing columns 211 in total, which are made of rubber. The bottom ends of the shock-absorbing columns 211 are fixed on the fixed base 201 with screws. The top of the column 211 is fixedly connected with screws, and the function of the shock-absorbing column 211 is to buffer the vibration generated by the operation of the upper mechanism, thereby ensuring the stability of the base and the whole device. The oscillation module also includes a configuration support block 209. The counterweight support block 209 is located under the eccentric bearing 208 and fixed on the motor shaft. The counterweight support block 209 rotates together with the eccentric bearing 208 and is always on the other side of the eccentric bearing 208 to ensure that The stability of the whole device is improved when the linear motor 202 rotates at high speed.

As shown in FIG. 5, the constant temperature module 102 specifically includes a heat conduction plate 301, a heat insulation plate 302, and a heating plate 303. The bottom of the heat insulation plate 302 is fixedly connected to the oscillation table 214, and the heating plate 303 is fixedly connected to the heat insulation plate 302 and the heat conduction plate 301 respectively. , There is an outer heat insulation frame around the heat conduction plate 301, and each of the four sides of the outer heat insulation frame has two protrusions for fixing the microplate. The heating plate 303 is an aluminum-based resistance heating plate with 132 SMD resistors welded on the surface. By changing the resistance value of the resistance, the heating power of the heating plate can be changed arbitrarily, and the heating power can be accurately calculated. A PT100 thermistor is also integrated on the surface of the aluminum-based resistance heating plate, which is used to monitor the temperature of the aluminum-based resistance heating plate in real time as a feedback signal for temperature control, so as to accurately control the temperature of the heating module. The aluminum-based resistance heating plate and the heat-conducting plate are coated with thermal-conductive silicone grease, and are tightly attached by screws to improve the heat conduction speed.

As shown in Figure 6, the auxiliary mechanism includes three boxes 103. The inside of the box is a hollow structure. The top of the box is placed with a microplate support. The microplate can be placed on it according to actual needs. The circuit board and the box are connected with the constant temperature oscillation mechanism.

The utility model cooperates with the mechanical gripper and the multi-channel pipette of the liquid transfer workstation to realize the automatic transfer of the microplate between the constant temperature oscillation incubation device and the liquid transfer workstation. The specific steps are as follows:

(1) Test sample preparation, prepare probe-modified magnetic beads, and extract and amplify the NDA product to be hybridized.

(2) Add probe-modified magnetic beads, hybridization solution, NDA product and pure water to the microplate with an eight-channel pipette of an automatic pipetting workstation.

(3) Use the mechanical gripper of the automatic liquid transfer workstation to transfer the microplate containing the sample to the constant temperature oscillation mechanism of the present utility model, and carry out the constant temperature oscillation incubation operation. The operation process is as follows:

a) Temperature - 95°C; time - 5 minutes; linear motor 202 rotates at 30 rpm;

b) Temperature - 55°C; time - 60 minutes; linear motor 202 rotates at 15 rpm.

(4) After the reaction is completed, add washing liquid, carry out two washing operations, each washing is shaken and mixed for 3 minutes, and the linear motor 202 rotates at 60 rpm. After the mixing is completed, the mechanical gripper transfers the microplate to a fully automatic pipetting workstation for magnetic separation to remove the supernatant.

(5) After the cleaning operation is completed, transfer the microplate to the constant temperature shaking mechanism again, add the blocking solution, shake and mix at room temperature for half an hour, the linear motor 202 rotates at 30 rpm, and then transfer to the magnetic separation mechanism for magnetic separation to remove the supernatant. .

(6) The hybridization process is completed, and samples are collected for subsequent detection operations.

Claims (10)

1. The utility model provides a device is hatched in constant temperature oscillation suitable for move liquid workstation which characterized in that: comprises a constant temperature oscillation mechanism and an auxiliary mechanism; the constant-temperature oscillating mechanism comprises an oscillating module (101) and a constant-temperature module (102), the oscillating module (101) comprises a fixed base (201) with a groove-shaped middle part, a linear motor (202) is fixedly connected with the fixed base (201), a motor shaft (203) is connected with the linear motor (202), an eccentric bearing (208) is fixed on the motor shaft (203), a first fixed table (204) and a second fixed table (205) are fixed on the fixed base (201), a lower sliding rod (210) penetrating through a middle sliding table (206) is fixed on the first fixed table (204) and the second fixed table (205), an upper sliding rod (209) penetrating through the upper sliding table (207) is fixed on the side wall of the fixed base (201), the upper sliding rod (209) is vertical to the lower sliding rod (210), the middle sliding table (206) and the upper sliding table (207) are in a shape like a Chinese character 'ji' and have a hollowed middle part, and the eccentric bearing (208) penetrates through the hollowed part of the upper sliding table (207), the eccentric bearing (208) is flush with the top of the upper sliding table (207), and is fixedly connected with the oscillating table (214), and the oscillating table (214) is fixedly connected with the upper sliding table (207); the constant temperature module arranged above the oscillation module can be used for placing a microporous plate; the auxiliary mechanism comprises a box body (103), the inside of the box body (103) is of a hollow structure, a microporous plate support (104) is placed at the top of the box body, and the box body (103) is connected with the constant-temperature oscillation mechanism.

2. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: four side walls of the middle sliding table (206) are provided with self-lubricating sleeves (213), the upper sliding rod (209) penetrates through the self-lubricating sleeves (213), and the lower sliding rod (210) penetrates through the self-lubricating sleeves (213) to be fixedly connected with the first fixing table (204) and the second fixing table (205) respectively.

3. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: the constant temperature module (102) comprises a heat insulation plate (302), a heating plate (303) and a heat conduction plate (301), the bottom of the heat insulation plate (302) is fixedly connected with the oscillating table (214), and the heating plate (303) is fixedly connected with the heat insulation plate (302) and the heat conduction plate (301) respectively; and a motor driver and a control circuit board are arranged in the cavity of the box body (103).

4. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: the oscillation module further comprises a damping column (211), the bottom end of the damping column (211) is fixed on the fixing base (201) through screws, and the top end of the damping column (211) is fixedly connected with the first fixing table (204) and the second fixing table (205) through screws respectively.

5. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: the eccentric bearing (208) has a protrusion at its upper end that mates with a recess in the middle of the oscillating table (214).

6. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: the upper sliding rod (209) and the lower sliding rod (210) are both provided with 2 sliding rods which are arranged in a # -shaped manner.

7. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: the oscillating module further comprises a configuration supporting block (209), and the counterweight supporting block (209) is positioned below the eccentric bearing (208) and fixed on the motor shaft (203).

8. The constant temperature shaking incubation device suitable for pipetting stations of claim 1, wherein: the periphery of the heat conducting plate (301) is also provided with an outer heat insulation frame (304).

9. The isothermal oscillating incubation device suitable for pipetting stations according to claim 8, characterized in that: the four sides of the outer insulating frame (304) have two protrusions (305) on each side.

10. The isothermal oscillating incubation device suitable for pipetting stations according to claim 3, characterized in that: heating plate (303) are aluminium base resistance heating plate, and there are chip resistor and temperature sensor on aluminium base resistance heating plate surface, scribble heat conduction silicone grease between aluminium base resistance heating plate and heat-conducting plate (301).

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