CN209974671U

CN209974671U - Antibody purification and separation device

Info

Publication number: CN209974671U
Application number: CN201920763222.7U
Authority: CN
Inventors: 李尾梅
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-05-25
Filing date: 2019-05-25
Publication date: 2020-01-21
Anticipated expiration: 2029-05-25

Abstract

The utility model discloses an antibody purification separator, including broken case, rough separation case, support frame and base, the both sides of base bottom all are provided with the rubber pad, and the top of base is provided with the support frame simultaneously, the inside both sides of support frame all are provided with the deposit chamber, and the both sides in deposit chamber all are provided with the spout, the top of support frame is fixed with broken case, and the top of broken case articulates there is the lid, and the edge on support frame top is provided with the handle ring simultaneously, the inside top of broken case is provided with the third vibration board, and the bottom of third vibration board is provided with the second vibration board. The utility model discloses a be provided with rough separation case and thin separator box, and the inside of rough separation case and thin separator box is provided with first filtration membrane and second filtration membrane respectively, utilizes first filtration membrane to carry out preliminary separation back to the tissue, passes through second filtration membrane separation again, and its double-deck isolating construction has improved the quality of separation.

Description

Antibody purification and separation device

Technical Field

The utility model relates to the technical field of biology, in particular to an antibody purification and separation device.

Background

The separation and purification refers to separating impurities in a mixture to improve the purity of the mixture, the preparation of a high-specificity and high-titer antibody is the basis of experimental immunology technology, the quality of the antibody directly influences the success or failure of a test, the antibody prepared by different methods is often mixed with a plurality of hybrid proteins, the antibody purification is needed in order to obtain the antibody with relatively single components or use the antibody for specific purposes, the analysis from the component perspective is carried out, the antibody molecule is a protein molecule, and like other proteins, the antibody molecule has certain isoelectric point, solubility, charge property and hydrophobicity, can be separated and purified by electrophoresis, salting-out precipitation or other chromatographic techniques, but the existing purification and separation device has many problems or defects:

firstly, the ultrasonic vibration crushing device of the traditional purification and separation device generally directly places tissues in the device for crushing, so that the crushing effect is poor, and the purification effect is low;

secondly, the separation device of the traditional purification and separation device usually adopts a plurality of separation membranes to realize purification and separation, the purification effect is poor, the separated liquid has impurities at all, and the filtering effect is extremely poor;

thirdly, the conventional purification and separation device is inconvenient for effectively preserving the separated antibody, and the antibody is easy to lose activity if the antibody cannot be temporarily preserved in time after being separated.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an antibody purification separator to solve the problem that the crushing effect that proposes among the above-mentioned background art is relatively poor, the purification effect is relatively poor and inconvenient to carry out the preservation to the antibody.

In order to achieve the above object, the utility model provides a following technical scheme: an antibody purification and separation device comprises a crushing box, a rough separation box, a support frame and a base, wherein rubber pads are arranged on two sides of the bottom end of the base, the support frame is arranged at the top end of the base, storage cavities are arranged on two sides of the interior of the support frame, sliding grooves are arranged on two sides of each storage cavity, the crushing box is fixed at the top end of the support frame, a cover body is hinged to the top end of the crushing box, a lifting ring is arranged at the edge of the top end of the support frame, a third vibrating plate is arranged at the top end of the interior of the crushing box, a second vibrating plate is arranged at the bottom end of the third vibrating plate, a first vibrating plate is arranged at the bottom end of the second vibrating plate, a first electromagnetic valve is arranged at the bottom end of the crushing box, the rough separation box is fixed at the bottom end of the first electromagnetic valve, a first filtering, one side of sealing plug all is provided with the limiting plate, and one side of thick separator box inside simultaneously evenly is provided with the draw-in groove, the bottom mounting of thick separator box has thin separator box, and the inside of thin separator box is fixed with second filtration membrane, the bottom of thin separator box is provided with the second solenoid valve, and one side of second solenoid valve is provided with the liquid outlet, and one side of support frame is provided with control panel simultaneously, and control panel's inside is provided with the singlechip, control panel's output passes through the input electric connection of wire and singlechip, the output of singlechip pass through the wire respectively with the input electric connection of supersonic generator, first solenoid valve and fixed slot.

Preferably, the two sides in the crushing box are provided with ultrasonic generators, and the ultrasonic generators are symmetrically distributed around the central line of the crushing box.

Preferably, a disassembly and assembly structure is arranged between the crushing box and the supporting frame.

Preferably, one side of the coarse separation box is provided with a slot matched with the sealing plug, and a sealing structure is formed between the slot and the sealing plug.

Preferably, all be provided with the test tube box between the spout, and the both sides of test tube box all are provided with the pulley of mutually supporting with the spout, be sliding structure between pulley and the spout.

Preferably, the both sides of second filtration membrane all are provided with the fixed slot, and the inside of fixed slot all is fixed with the retainer plate, be the joint structure between retainer plate and the second filtration membrane.

Compared with the prior art, the beneficial effects of the utility model are that: the antibody purification and separation device has a reasonable structure and has the following advantages:

1. through inside at broken case be provided with, second vibration board and third vibration board, place the histiocyte back in the broken case, after supersonic generator started, the cell on third vibration board surface received supersonic generator's influence and flowed to the surface of second vibration board after being smashed, further smash, treat that histiocyte volume is more tiny back, flow to the surface of first vibration board from the surface of second vibration board and smash once more, its crushing effect is better.

2. Through being provided with rough separation case and thin separator box, and the inside of rough separation case and thin separator box is provided with first filtration membrane and second filtration membrane respectively, utilizes first filtration membrane to carry out preliminary separation back to the tissue, passes through the separation of second filtration membrane again, and its double-deck isolating construction has improved the quality of separation.

3. Through being provided with the test tube box, the antibody cell that the separation was accomplished is preserved in the test tube to place and temporarily store in the test tube box, ensure antibody cell's activity.

Drawings

Fig. 1 is a schematic front view of a cross-sectional structure of the present invention;

FIG. 2 is a schematic view of the front sectional structure of the rough separation box of the present invention;

FIG. 3 is a schematic view of the front cross-sectional structure of the thin separator box of the present invention;

fig. 4 is a system block diagram of the present invention.

In the figure: 1. a cover body; 2. a crushing box; 3. an ultrasonic generator; 4. a first solenoid valve; 5. a coarse separation box; 6. a support frame; 7. a test tube box; 8. a pulley; 9. a base; 10. a rubber pad; 11. a chute; 12. a fine separation tank; 13. a control panel; 14. a single chip microcomputer; 15. a reservoir chamber; 16. a limiting plate; 17. lifting a ring; 18. a first vibrating plate; 19. a second vibration plate; 20. a third vibrating plate; 21. a card slot; 22. a first filter membrane; 23. a slot; 24. a sealing plug; 25. a stationary ring; 26. a second filter membrane; 27. a liquid outlet; 28. a second solenoid valve; 29. and fixing the grooves.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides an embodiment: an antibody purification and separation device comprises a crushing box 2, a rough separation box 5, a support frame 6 and a base 9, wherein rubber pads 10 are arranged on two sides of the bottom end of the base 9, the support frame 6 is arranged on the top end of the base 9, storage cavities 15 are arranged on two sides inside the support frame 6, sliding chutes 11 are arranged on two sides of each storage cavity 15, a test tube box 7 is arranged between each sliding chute 11, pulleys 8 matched with the sliding chutes 11 are arranged on two sides of each test tube box 7, a sliding structure is formed between each pulley 8 and each sliding chute 11, so that test tubes are convenient to take out, the crushing box 2 is fixed on the top end of the support frame 6, a cover body 1 is hinged to the top end of the crushing box 2, a disassembly and assembly structure is formed between the crushing box 2 and the support frame 6, so that maintenance is convenient, lifting rings 17 are arranged on the edge of the top end of the support frame 6, the model of the ultrasonic generator 3 can be ZC58-25C-0.8, the ultrasonic generator 3 is symmetrically distributed about the center line of the crushing box 2, the crushing efficiency is improved, the top end of the inside of the crushing box 2 is provided with a third vibrating plate 20, the bottom end of the third vibrating plate 20 is provided with a second vibrating plate 19, the bottom end of the second vibrating plate 19 is provided with a first vibrating plate 18, the bottom end of the crushing box 2 is provided with a first electromagnetic valve 4, the bottom end of the first electromagnetic valve 4 is fixed with a rough separation box 5, the inside of the rough separation box 5 is uniformly provided with a first filtering membrane 22, one side of the first filtering membrane 22 is provided with a sealing plug 24, one side of the sealing plug 24 is provided with a limiting plate 16, one side of the inside of the rough separation box 5 is uniformly provided with a clamping groove 21, one side of the rough separation box 5 is provided with a slot, and a sealing structure is formed between the slot 23 and the sealing plug 24, so that the tissue cells are prevented from seeping, the bottom end of the coarse separation box 5 is fixed with the fine separation box 12, the interior of the fine separation box 12 is fixed with the second filtering membrane 26, both sides of the second filtering membrane 26 are provided with fixing grooves 29, the model of the fixing grooves 29 can be ZC58-25C-0.6, the interior of the fixing grooves 29 is fixed with a fixing ring 25, a clamping structure is formed between the fixing ring 25 and the second filtering membrane 26, so that the second filtering membrane 26 is convenient to clean, the bottom end of the fine separation box 12 is provided with a second electromagnetic valve 28, one side of the second electromagnetic valve 28 is provided with a liquid outlet 27, one side of the support frame 6 is provided with the control panel 13, the interior of the control panel 13 is provided with the single chip microcomputer 14, the model of the single chip microcomputer 14 can be HT66F018, the output end of the, the output end of the single chip microcomputer 14 is electrically connected with the ultrasonic generator 3, the first electromagnetic valve 4 and the input end of the fixing groove 29 through conducting wires.

The working principle is as follows: the device adopts chromatography to separate and purify the antibody, when in use, firstly, the histiocyte is placed into a crushing box 2 from a cover body 1, an ultrasonic generator 3 is started, the histiocyte on the surface of a third vibrating plate 20 is crushed under the influence of the ultrasonic generator 3, the crushed histiocyte flows to the surface of a second vibrating plate 19 and is further crushed, after the histiocyte is smaller in volume, the histiocyte flows to the upper part of a first vibrating plate 18 from a through hole on the surface of the second vibrating plate 19 and is crushed again, the histiocyte crushed for three times flows into a coarse separation box 5 from a first electromagnetic valve 4 to be primarily separated, the histiocyte separated by a first filtering membrane 22 flows into a fine separation box 12 to be further separated and purified, then, the second electromagnetic valve 28 is opened, the antibody separated by a second filtering membrane 26 flows out from a liquid outlet 27 and is collected by a test tube in time, at last, the test tube with the antibody is placed in the test tube box 7 for temporary storage, so that the activity of the antibody is guaranteed.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides an antibody purification separator, includes broken case (2), coarse separation case (5), support frame (6) and base (9), its characterized in that: rubber pads (10) are arranged on two sides of the bottom end of the base (9), a supporting frame (6) is arranged on the top end of the base (9), storage cavities (15) are arranged on two sides of the interior of the supporting frame (6), sliding grooves (11) are arranged on two sides of each storage cavity (15), a crushing box (2) is fixed on the top end of the supporting frame (6), a cover body (1) is hinged to the top end of the crushing box (2), a lifting ring (17) is arranged on the edge of the top end of the supporting frame (6), a third vibrating plate (20) is arranged on the top end of the interior of the crushing box (2), a second vibrating plate (19) is arranged on the bottom end of the third vibrating plate (20), a first vibrating plate (18) is arranged on the bottom end of the second vibrating plate (19), a first electromagnetic valve (4) is arranged on the bottom end of the crushing box (2), and a rough separation box (5, the inner part of the coarse separation box (5) is uniformly provided with a first filtering membrane (22), one side of the first filtering membrane (22) is provided with a sealing plug (24), one side of the sealing plug (24) is provided with a limiting plate (16), one side of the inner part of the coarse separation box (5) is uniformly provided with a clamping groove (21), the bottom end of the coarse separation box (5) is fixed with a fine separation box (12), the inner part of the fine separation box (12) is fixed with a second filtering membrane (26), the bottom end of the fine separation box (12) is provided with a second electromagnetic valve (28), one side of the second electromagnetic valve (28) is provided with a liquid outlet (27), one side of the support frame (6) is provided with a control panel (13), the inner part of the control panel (13) is provided with a single chip microcomputer (14), the output end of the control panel (13) is electrically connected with the input end of the, the output end of the single chip microcomputer (14) is electrically connected with the input ends of the ultrasonic generator (3), the first electromagnetic valve (4) and the fixing groove (29) through conducting wires.

2. The apparatus for purifying and separating antibody according to claim 1, wherein: the two sides in the crushing box (2) are provided with ultrasonic generators (3), and the ultrasonic generators (3) are symmetrically distributed about the central line of the crushing box (2).

3. The apparatus for purifying and separating antibody according to claim 1, wherein: and a dismounting and mounting structure is arranged between the crushing box (2) and the supporting frame (6).

4. The apparatus for purifying and separating antibody according to claim 1, wherein: and one side of the coarse separation box (5) is provided with a slot (23) which is matched with the sealing plug (24), and a sealing structure is formed between the slot (23) and the sealing plug (24).

5. The apparatus for purifying and separating antibody according to claim 1, wherein: all be provided with test tube box (7) between spout (11), and the both sides of test tube box (7) all are provided with pulley (8) of mutually supporting with spout (11), be sliding structure between pulley (8) and spout (11).

6. The apparatus for purifying and separating antibody according to claim 1, wherein: both sides of second filtration membrane (26) all are provided with fixed slot (29), and the inside of fixed slot (29) all is fixed with retainer plate (25), be the joint structure between retainer plate (25) and second filtration membrane (26).