CN209317703U - A Centrifugal Microfluidic Chip for Rapid Detection of Trace Substances - Google Patents

Abstract

The utility model relates to a kind of centrifugal type microfludic chips quickly detected for trace materials.Including No.1 injection port, No. two injection ports, No. three injection ports, No.1 card slot, reaction tank, reaction channel, detection cell, venthole, waste liquid pool, No. two card slots, No. three card slots, No. four card slots；The No.1, No. two, No. three injection ports close to the center of circle, the detection cell, venthole, waste liquid pool are connected to U-shaped close to chip edge, and the No.1, No. two, No. three injection ports and reaction tank, reaction channel, detection cell, venthole, waste liquid pool are sequentially connected；Reaction channel is made of four V-structures, and channel two sides outer wall inwardly has protrusion；Wherein No.1 card slot is located at chip the center point, No. two, No. three, No. four card slots be distributed in three apexes of the equilateral triangle centered on the center of circle.The utility model can make plurality of reagents be sufficiently mixed reaction while realizing high-throughput multi-channel detection trace materials, and can guarantee that micro-fluidic chip high speed stablizes rotation.

Description

A Centrifugal Microfluidic Chip for Rapid Detection of Trace Substances

technical field

The utility model belongs to the field of microfluidics, in particular to a centrifugal microfluidics chip for rapid detection of trace substances.

Background technique

In modern life, the detection of trace substances plays an important role in the food industry, environmental monitoring, medical care, and disease diagnosis. Commonly used detection methods include spectrophotometry, gas chromatography, etc., among which spectrophotometry The detection accuracy is low, and usually only qualitative measurement can be achieved; gas chromatography has disadvantages such as high cost of use and small scope of application. Microfluidic chip is a technology that integrates the basic operation units of sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis into a micron-scale chip, and automatically completes the entire analysis process. As a micro-analysis experimental device, the microfluidic chip has become the first choice for the detection of trace substances in the fields of life sciences, chemistry, and food safety in recent years due to its advantages of miniaturization, automation, integration, high throughput, and low cost. method. For example, the patent application number is 201820087187.7, and the patent name is a utility model patent for a microfluidic disk based on the combination of Y-type and S-type channels. The chip has few detection units and cannot achieve high-throughput detection; the patent application number is 201610045231.3, The patent name is the invention patent of microfluidic chip and its application in pesticide detection. The structure of the reaction channel of the chip is simple, which will lead to insufficient reagent reaction; for example, the patent application number is 201620365855.9, and the patent name is a compound centrifugal food The utility model patent of the microfluidic chip for additive detection has a small and only one card slot, which has the disadvantage of unstable high-speed centrifugation. At present, microfluidic chips used in the field of trace substance detection generally have the problems of simple channel structure and unstable high-speed rotation.

Contents of the invention

In view of the above-mentioned problems existing in the prior art, the purpose of this utility model is to provide a centrifugal microfluidic chip for rapid detection of trace substances, the purpose of which is to fully mix various reagents while achieving high throughput reaction, and the microfluidic chip can rotate stably under high-speed centrifugation.

In order to solve the above problems, the utility model adopts the following technical solutions:

A centrifugal microfluidic chip for rapid detection of trace substances, including 9 detection units, the detection unit includes No. 1 inlet (1), No. 2 inlet (2), and No. 3 inlet (3), No. 1 card slot (4), reaction pool (5), reaction channel (6), detection pool (7), vent hole (8), waste liquid pool (9), No. 2 card slot (10) , No. 3 card slot (11), No. 4 card slot (12); said No. 1 injection port (1), No. 2 injection port (2), and No. 3 injection port (3) are close to the center of the circle, and said The detection pool (7), vent hole (8), and waste liquid pool (9) are connected to form a U shape close to the edge of the chip. The No. 1 injection port (1), No. 2 injection port (2), and No. The port (3) is connected to one end of the reaction pool (5), the other end of the reaction pool (5) is connected to the reaction channel (6), the reaction channel (6) is connected to the detection pool (7), the detection pool (7), the air hole ( 8), the waste liquid pool (9) is connected in sequence, the characteristic is that the reaction channel (6) is composed of four V-shaped structures, and the outer walls on both sides of the channel have protrusions inward; the No. 1 card slot (4) is located in the center of the chip circle , the No. 2 card slot (10), the No. 3 card slot (11), and the No. 4 card slot (12) are distributed at the three vertices of an equilateral triangle centered on the center of the circle. The microfluidic chip consists of three layers of microstructured substrates engraved with micron scale, in which the first chip layer is on the top, the second chip layer is in the middle, and the third chip layer is on the bottom. Inlet No. 1 (1), Inlet No. 2 (2), Inlet No. 3 (3), Card slot No. 1 (4), Card slot No. 2 (10), Card slot No. 3 (11) , the No. 4 card slot (12), and the vent hole (8) run through the first chip layer. Inlet No. 1 (1), Inlet No. 2 (2), Inlet No. 3 (3), Card Slot No. 1 (4), Reaction Pool (5), Reaction Channel (6), Detection Pool ( 7), vent hole (8), waste liquid pool (9), No. 2 card slot (10), No. 3 card slot (11), and No. 4 card slot (12) run through the second chip layer. The first card slot (4), the second card slot (10), the third card slot (11), and the fourth card slot (12) run through the third chip layer. No. 1 injection port (1), No. 2 injection port (2), No. 3 injection port (3), air hole (8), and waste liquid tank (9) are circular with a diameter of 2 mm; Card slot (4), No. 2 card slot (10), No. 3 card slot (11), and No. The side length of the equilateral triangle surrounded by the card slot (11) and the fourth card slot (12) is 6 mm; the reaction pool (5) is hexagonal, with a length of 8 mm, a width of 5 mm, and an upper vertex angle of 60°; The width of the reaction channel (6) is 300µm, and the height of the protrusion is 150µm; the detection cell (7) is hexagonal, with a length of 9mm, a width of 6mm, and an upper angle of 120°. The thicknesses of the first chip layer, the second chip layer and the third chip layer are respectively 0.5 mm, 1 mm and 0.5 mm, and the material thereof is PMMA.

The beneficial effects of the utility model are:

1. This microfluidic chip has a reaction pool and multiple V-shaped reaction channels, and there are protrusions in the channel, which reduces the channel width and slows down the liquid flow rate, so as to achieve the effect of sufficient mixing;

2. The microfluidic chip has four card slots and is relatively dispersed, which can make the microfluidic chip stably fixed on the centrifuge, and can rotate stably under high-speed centrifugation;

3. The microfluidic chip is distributed in a circular array and has 9 detection pools, which can detect multiple samples at the same time.

Description of drawings

Below in conjunction with accompanying drawing and embodiment thereof, the utility model is further described.

Fig. 1 is a structural schematic diagram of a microfluidic chip;

Fig. 2 is a structural schematic diagram of the first chip layer;

Fig. 3 is a structural schematic diagram of the second chip layer;

Fig. 4 is a schematic structural diagram of a third chip layer;

Fig. 5 is an enlarged view of the partial structure of the second chip layer;

1 is the first injection port, 2 is the second injection port, 3 is the third injection port, 4 is the first card slot, 5 is the reaction pool, 6 is the reaction channel, 7 is the detection pool, and 8 is the air hole , 9 is the waste liquid pool, 10 is the No. 2 card slot, 11 is the No. 3 card slot, and 12 is the No. 4 card slot.

Detailed ways

Embodiment: The embodiment of Z-shaped passage engraved with 3 protrusions

In Figures 1, 3, and 5, a centrifugal microfluidic chip for rapid detection of trace substances includes 9 detection units, and the detection units include No. 1 inlet 1, No. 2 inlet 2, and No. No. 3 inlet, No. 1 slot 4, Reaction pool 5, Reaction channel 6, Detection pool 7, Air vent 8, Waste liquid pool 9, No. 2 slot 10, No. 3 slot 11, No. 4 slot 12 ; The No. 1 sampling port 1, No. 2 sampling port 2, and No. 3 sampling port 3 are circular, with a diameter of 2 mm and close to the center of the circle. The detection pool 7, the vent hole 8, and the waste liquid pool 9 are connected Become U-shaped near the edge of the chip, wherein the detection pool 7 is a hexagon, with a length of 9 mm, a width of 6 mm, and an upper angle of 120 °. The vent hole 8 and the waste liquid pool 9 are circular and have a diameter of 2 mm. No. 1 inlet 1, No. 2 inlet 2, and No. 3 inlet 3 are connected to one end of the reaction pool 5, wherein the reaction pool 5 is hexagonal, with a length of 8 mm, a width of 5 mm, and an upper vertex angle of 60° , the other end of the reaction pool 5 is connected to the reaction channel 6, wherein the width of the reaction channel 6 is 300 μm, the reaction channel 6 is connected to the detection pool 7, and the detection pool 7, the vent hole 8, and the waste liquid pool 9 are connected in sequence; the reaction channel 6 is composed of four Composed of a V-shaped structure, and the outer walls on both sides of the channel have protrusions inward, and the height of the protrusions is 150µm; the first card slot 4 is located at the center of the chip circle, the second card slot 10, the third card slot 11, and the fourth card slot 12 Distributed at the three vertices of an equilateral triangle centered on the center of the circle, the four card slots are regular hexagons with a side length of 0.5mm, the second card slot 10, the third card slot 11, and the fourth card slot 12 The side length of the equilateral triangle formed is 6mm;

In Fig. 2, No. 1 inlet 1, No. 2 inlet 2, No. 3 inlet 3, No. 1 card slot 4, No. 2 card slot 10, No. 3 card slot 11, No. 4 card slot 12, The air hole 8 runs through the first chip layer;

In Figure 3, No. 1 inlet 1, No. 2 inlet 2, No. 3 inlet 3, No. 1 card slot 4, reaction pool 5, reaction channel 6, detection pool 7, air hole 8, waste liquid pool 9. The second card slot 10, the third card slot 11, and the fourth card slot 12 run through the second chip layer;

In Figure 4, No. 1 inlet 1, No. 2 inlet 2, No. 3 inlet 3, No. 1 card slot 4, reaction pool 5, reaction channel 6, detection pool 7, air hole 8, and waste liquid pool 9. The second card slot 10, the third card slot 11, and the fourth card slot 12 run through the third chip layer;

The thicknesses of the first chip layer, the second chip layer, and the third chip layer are 0.5 mm, 1 mm, and 0.5 mm, respectively;

The method for preparing channel protrusions in this embodiment: using a carbon dioxide laser engraving machine to engrave on a substrate made of PMMA.

The working process of a centrifugal microfluidic chip for rapid detection of trace substances (taking surface-enhanced Raman rapid detection technology as an example):

1. According to the structural connection shown in Figure 3, inject the sample to be tested, the detection aid reagent and the noble metal nanoparticles from the No. 1 injection port 1, the No. 2 injection port 2, and the No. 3 injection port 3;

2. Put the microfluidic chip into the centrifuge, set the centrifugal speed to 250~500rpm, and the direction of rotation is clockwise, start the centrifuge, the reagents enter the reaction pool 5 and start the mixed reaction;

3. After the reagent enters the reaction channel 6, increase the centrifugal speed to 800~1500rpm, and the rotation direction is counterclockwise, and finally the reagent flows into the detection pool 7 and the vent hole 8. The detection cell 7 is irradiated with a Raman probe to obtain Raman data of the analyte, thereby performing qualitative and quantitative detection.

This embodiment and the examples of the working process are preferred solutions, and simple changes to the number of protrusions all belong to the scope of protection of the present invention.

Claims (7)

1. A centrifugal microfluidic chip for rapid detection of trace substances, including 9 detection units, the detection unit includes No. 1 inlet (1), No. 2 inlet (2), No. 3 inlet Sample port (3), No. 1 card slot (4), reaction pool (5), reaction channel (6), detection pool (7), vent hole (8), waste liquid pool (9), No. 2 card slot ( 10), No. 3 card slot (11), No. 4 card slot (12); said No. 1 injection port (1), No. 2 injection port (2), and No. 3 injection port (3) are close to the center of the circle, The detection pool (7), vent hole (8), and waste liquid pool (9) are connected to form a U shape close to the edge of the chip, and the No. 1 sample inlet (1), No. 2 sample inlet (2), No. The sample inlet (3) is connected to one end of the reaction pool (5), the other end of the reaction pool (5) is connected to the reaction channel (6), the reaction channel (6) is connected to the detection pool (7), and the detection pool (7), through The air hole (8) and the waste liquid pool (9) are connected in sequence. The characteristic is that the reaction channel (6) is composed of four V-shaped structures, and the outer walls on both sides of the channel have protrusions inward; the No. 1 card slot (4) is located At the center of the chip circle, the No. 2 card slot (10), the No. 3 card slot (11), and the No. 4 card slot (12) are distributed at three vertices of an equilateral triangle centered on the center of the circle. 2. A centrifugal microfluidic chip for rapid detection of trace substances according to claim 1, wherein the microfluidic chip is composed of three layers of microstructure substrates engraved with micron levels, wherein the first One chip layer is on top, the second chip layer is in the middle, and the third chip layer is on the bottom. 3. A centrifugal microfluidic chip for rapid detection of trace substances according to claim 1, characterized in that the No. 1 inlet (1), the No. 2 inlet (2), and the No. The sample port (3), No. 1 card slot (4), No. 2 card slot (10), No. 3 card slot (11), No. 4 card slot (12), and air holes (8) run through the first chip layer. 4. A centrifugal microfluidic chip for rapid detection of trace substances according to claim 1, characterized in that the No. 1 inlet (1), the No. 2 inlet (2), and the No. Sample port (3), No. 1 card slot (4), reaction pool (5), reaction channel (6), detection pool (7), vent hole (8), waste liquid pool (9), No. 2 card slot ( 10), No. 3 card slot (11), and No. 4 card slot (12) run through the second chip layer. 5. A centrifugal microfluidic chip for rapid detection of trace substances according to claim 1, characterized in that the No. 1 card slot (4), the No. 2 card slot (10), and the No. 3 card slot ( 11), No. 4 card slot (12) runs through the third chip layer. 6. A centrifugal microfluidic chip for rapid detection of trace substances according to claim 1, characterized in that the No. 1 inlet (1), the No. 2 inlet (2), and the No. The sample port (3), vent hole (8), and waste liquid pool (9) are circular with a diameter of 2 mm; No. 1 card slot (4), No. 2 card slot (10), and No. 3 card slot (11) 1. The No. 4 card slot (12) is a regular hexagon with a side length of 0.5mm; the sides of the equilateral triangle surrounded by the No. 2 card slot (10), the No. 3 card slot (11) and the No. 4 card slot (12) The length is 6mm; the reaction cell (5) is hexagonal, with a length of 8mm, a width of 5mm, and an upper angle of 60°; the width of the reaction channel (6) is 300µm, and the height of the protrusion is 150µm; the detection cell (7) is The hexagon is 9mm long, 6mm wide, and has an upper vertex angle of 120°. 7. A kind of centrifugal microfluidic chip for rapid detection of trace substances according to claim 1, characterized in that the thicknesses of the first chip layer, the second chip layer, and the third chip layer are respectively 0.5mm, 1mm, 0.5mm, and its material is PMMA.