CN209193857U - A kind of albumen precipitation supernatant separation device - Google Patents

Abstract

The utility model provides a protein precipitation supernatant liquid separation device. Specifically, the utility model provides a protein precipitation supernatant separation device, the device includes a sedimentation tank, a gas source, the gas source is connected to the sedimentation tank through a gas pipeline; the sedimentation tank The tank wall is provided with a feed pipe, a discharge pipe, a supernatant separator, and a window; the supernatant separator includes a sleeve and a sampling tube, and the sleeve passes through the settling tank On the upper tank wall, the sampling tube passes through the sleeve, the sampling tube is sealed and connected to the sleeve and can slide freely, the lower end of the sampling tube in the sedimentation tank is bent to be parallel to the horizontal plane, The nozzle of the sampling pipe in the sedimentation tank is provided with a 30-60° upper bevel, and the nozzle of the sampling pipe outside the sedimentation tank is provided with a diaphragm valve. The protein precipitation supernatant liquid separation device of the utility model can realize rapid separation of the protein precipitation supernatant liquid, has simple operation and ensures product quality.

Description

A protein precipitation supernatant separation device

technical field

The utility model relates to the field of biopharmaceutical equipment, in particular to a protein precipitation supernatant liquid separation device.

Background technique

In the separation and purification of biopharmaceutical proteins, most of them use the process of protein precipitation for preliminary separation. However, in the large-scale production process, after the protein is fully precipitated, a large amount of supernatant in the material tank needs to be removed. At present, it is often used to install a hose under the top of the tank, and then use a peristaltic pump to extract the outside of the tank. The disadvantage of this is that it is easy to suck up the sediment at the bottom, which affects the separation effect, and this separation method It is easy to lead to the contamination of bacteria, thus polluting the protein.

Therefore, there is a need in the art to develop a protein precipitation supernatant separation device that is fast, easy to operate and ensures product quality requirements.

Utility model content

The purpose of the utility model is to provide a protein precipitation supernatant liquid separation device which is fast, easy to operate and guarantees product quality requirements.

The first aspect of the present utility model provides a protein precipitation supernatant liquid separation device, the device includes a precipitation tank 10, a gas source 20, and the gas source is connected to the precipitation tank through a gas pipeline 30;

The tank wall of the settling tank is provided with a feed pipe 11, a discharge pipe 12, a supernatant liquid separator, and a window 13;

Described supernatant separator comprises sleeve pipe 14 and sampling pipe 15, and described sleeve pipe passes the upper tank wall of described settling tank, and described sampling pipe passes through described sleeve pipe, and described sampling pipe and The sleeve pipes are sealed and connected and can slide freely. The lower end of the sampling pipe in the sedimentation tank is bent to be parallel to the horizontal plane. The nozzle of the sampling pipe outside the sedimentation tank is provided with a diaphragm valve 16 .

In another preferred example, the lower end of the sampling pipe in the sedimentation tank is vertically bent to be parallel to the horizontal plane.

In another preferred example, the discharge pipe is located at the bottom of the sedimentation tank.

In another preferred example, the length of the casing is the same as the wall thickness of the upper tank wall of the sedimentation tank.

In another preferred example, the casing is fixedly connected to the upper tank wall of the sedimentation tank.

In another preferred example, the gas source is a compressed air source.

In another preferred example, the bottom of the sedimentation tank is a conical structure, and the discharge pipe is arranged at the bottom of the sedimentation tank.

In another preferred example, the tapered structure is an arc-shaped tapered structure.

In another preferred example, the windows are arranged longitudinally on the tank wall of the sedimentation tank.

In another preferred example, the window is a transparent window.

In another preferred example, the window is a transparent glass window.

In another preferred example, both the sampling tube and the sleeve are hard tubes.

In another preferred example, the diaphragm valve is a sanitary diaphragm valve.

In another preferred example, the settling tank is provided with a height-adjustable support frame for placing the sampling tube.

In another preferred example, a liquid filter 17 is also provided on the sampling pipe outside the settling tank.

In another preferred example, the air transmission pipeline is further provided with an air filter 31 .

In another preferred example, both the feed pipe and the discharge pipe are provided with a valve 18 .

In another preferred example, the precipitation tank is further provided with a heater.

It should be understood that within the scope of the present utility model, the above-mentioned technical features of the present utility model and the technical features specifically described in the following (such as embodiments) can be combined with each other to form new or preferred technical solutions . Due to space limitations, we will not repeat them here.

Description of drawings

The accompanying drawings are for illustrative purposes only, and should not be construed as limitations on this patent; in order to better illustrate this embodiment, certain components in the accompanying drawings will be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art For personnel, it is understandable that some known structures and descriptions thereof may be omitted in the drawings; the same or similar symbols correspond to the same or similar components; as a limitation of this patent.

Fig. 1 is a structural schematic diagram of a protein precipitation supernatant separation device in a preferred example of the present invention.

Among them, each number in the accompanying drawings represents:

10 is the settling tank, 11 is the feed pipe, 12 is the discharge pipe, 13 is the window, 14 is the casing, 15 is the sampling pipe, 16 is the diaphragm valve, 17 is the liquid filter, 18 is the valve, 20 is the gas source , 30 is an air pipeline and 31 is an air filter.

Detailed ways

The inventor has developed a protein precipitation supernatant separation device through extensive and in-depth research. In the protein precipitation supernatant separation device described in the utility model, the sampling tube is sealed and connected with the sleeve tube and can slide freely, therefore, the height of the mouth of the sampling tube in the sedimentation tank can be adjusted to make the The nozzle is located at the height of the interface between the supernatant and the precipitated protein, and the gas source is used to pressurize the precipitation tank, and the supernatant is discharged through the action of the air pressure, thereby realizing the separation of the supernatant of the protein precipitation, as described in the utility model The advanced protein precipitation supernatant separation device has the advantages of rapid separation, simple operation and guaranteed product quality requirements. On this basis, the inventor has completed the utility model.

the term

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs.

As used herein, the terms "comprising", "comprising" and "containing" are used interchangeably to include not only open definitions, but also semi-closed, and closed definitions. In other words, the terms include "consisting of", "consisting essentially of".

As used herein, the orientation or positional relationship indicated by the terms "upper", "lower", "top", "bottom", "horizontal" etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention. The new inventions and simplified descriptions do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the utility model.

As used herein, the term "connection" should be understood in a broad sense, which may be a fixed connection, a detachable connection, or an integral connection.

As used herein, the term "protein precipitation supernatant separation device" and "device" are used interchangeably.

For the convenience of description, the utility model will be further described below in conjunction with accompanying drawing 1. It should be understood in the utility model that the accompanying drawing does not limit the scope of the utility model.

Typically, the utility model provides a protein precipitation supernatant separation device, which is characterized in that the device includes a sedimentation tank 10, a gas source 20, and the gas source is connected to the sedimentation tank through a gas pipeline 30 connected;

The tank wall of the settling tank is provided with a feed pipe 11, a discharge pipe 12, a supernatant liquid separator, and a window 13;

Described supernatant separator comprises sleeve pipe 14 and sampling pipe 15, and described sleeve pipe passes the upper tank wall of described settling tank, and described sampling pipe passes through described sleeve pipe, and described sampling pipe and The sleeve pipes are sealed and connected and can slide freely. The lower end of the sampling pipe in the sedimentation tank is bent to be parallel to the horizontal plane. The nozzle of the sampling pipe outside the sedimentation tank is provided with a diaphragm valve 16 .

In the device described in the utility model, the supernatant and the interface of precipitating protein are observed through the window provided on the tank wall of the sedimentation tank. Preferably, the window is a transparent window, typically, the window is a transparent glass window. In another preferred example, the windows are arranged longitudinally on the tank wall of the sedimentation tank.

In the protein separation and purification of biopharmaceuticals, the amount of material in each precipitation tank is often different, resulting in different interface heights between the supernatant and the precipitated protein each time. In the protein precipitation supernatant separation device described in the utility model, the The above-mentioned sampling pipe is sealed and connected with the sleeve pipe and can slide freely. Therefore, the height of the mouth of the sampling pipe in the sedimentation tank can be adjusted through the sliding between the sampling pipe and the sleeve pipe, so that the sampling pipe in the sedimentation tank The nozzle is located between the supernatant and precipitated protein interface, so as to achieve the separation requirements of supernatant and precipitated protein with different interface heights.

In the device described in the utility model, the nozzle of the sampling pipe in the settling tank is provided with a 30-60° upper inclined section. Compared with the traditional circular nozzle, the nozzle is equipped with a 30-60° upward slope, which can not only effectively increase the area of the nozzle, increase the discharge rate of the supernatant, and prevent the nozzle from being blocked, but also has a 30-60° upward slope. The nozzle of the cutting surface is adapted to the hydrodynamics of the supernatant to ensure the smooth flow of the supernatant and prevent the abnormal flow of the supernatant from causing the protein precipitate to be sucked up and affect the separation effect.

In the device described in the utility model, the gas source is connected to the sedimentation tank through the gas pipeline, and the gas can be passed into the sedimentation tank through the gas source to pressurize the supernatant in the sedimentation tank, The role of the supernatant was discharged. Compared with the traditional installation of a hose on the top of the sedimentation tank, and then use a peristaltic pump to extract the outside of the tank (the suction of the traditional peristaltic pump often acts directly on the precipitated protein during the suction process, thus causing the precipitated protein at the bottom to be sucked up) Compared with the device described in the utility model, the air pressure directly acts on the supernatant and the pressure of the gas on the supernatant is evenly pressurized, which can effectively prevent the precipitated protein from being sucked up and ensure the separation effect.

In the device described in the present utility model, the sampling tube and the casing are sealed and connected, and the sealed connection can ensure that bacteria in the air cannot enter the sedimentation tank during the sliding process of the sampling tube and the casing. Contaminate the sedimentation tank. In a preferred example, the diaphragm valve is a sanitary diaphragm valve. In another preferred example, a liquid filter 17 is also provided on the sampling pipe outside the settling tank. In another preferred example, the air transmission pipeline is also provided with an air filter. The setting of sanitary diaphragm valve, liquid filter and/or air filter can further prevent miscellaneous bacteria from entering the precipitation tank during the separation process, so that the precipitated protein in the precipitation tank is polluted by microorganisms, thereby ensuring the quality of the precipitated protein.

In a preferred example of the present utility model, the bottom of the sedimentation tank is a conical structure, and the discharge pipe is arranged at the bottom of the sedimentation tank. Compared with the traditional sedimentation tank with a flat bottom, the sedimentation tank with a conical bottom can ensure the smooth flow of precipitated protein from the discharge pipe, avoid incomplete collection of precipitated protein, and improve the collection rate of precipitated protein.

In another preferred example, both the feed pipe and the discharge pipe are provided with a valve 18 .

In another preferred example, both the sampling tube and the sleeve are hard tubes.

In another preferred example, the settling tank is provided with a height-adjustable support frame for placing the sampling tube.

In another preferred example, the air transmission pipeline is further provided with an air filter 31 .

In another preferred example, the precipitation tank is further provided with a heater. The heater heats the material in the sedimentation tank to ensure that the temperature during the supernatant liquid separation process is kept within a certain range, which can prevent some supernatant liquid from being unfavorable for discharge due to the increase in viscosity caused by the temperature drop.

The main advantages of the utility model include:

1. The protein precipitation supernatant liquid separation device described in the utility model can flexibly adapt to the separation of supernatant liquid and precipitated protein at different interface heights.

2. The device described in the utility model discharges the supernatant by means of gas pressurization, which can effectively prevent the precipitated protein from being sucked up and ensure the separation effect.

3. The device described in the utility model can prevent the sedimentation tank from being polluted by microorganisms, thereby ensuring the quality of the separated precipitated protein.

Below in conjunction with specific embodiment, further set forth the utility model. It should be understood that these embodiments are only used to illustrate the present utility model and are not intended to limit the scope of the present utility model. For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed. Percentages and parts are by weight unless otherwise indicated.

Example 1

This embodiment provides a protein precipitation supernatant separation device, and the protein precipitation supernatant separation device includes:

The device includes a settling tank and a gas source, the gas source is connected to the settling tank through a gas pipeline, and the gas pipeline is also provided with an air filter;

The bottom of the settling tank is a conical structure, the discharge pipe is arranged on the bottom of the settling tank, and the tank wall of the settling tank is provided with a feed pipe, a discharge pipe, a supernatant separator, A window and a heater, the window is a transparent glass window and is arranged longitudinally on the tank wall of the sedimentation tank, and the feed pipe and the discharge pipe are all provided with valves;

Described supernatant liquid separator comprises sleeve pipe and sampling pipe, and described sleeve pipe passes through the upper tank wall of described sedimentation tank, and described sampling pipe passes through described sleeve pipe, and described sampling pipe and described The sleeves are tightly connected and can slide freely. The lower end of the sampling tube in the sedimentation tank is bent to be parallel to the horizontal plane. The mouth of the pipe is provided with a sanitary diaphragm valve, and the sampling pipe outside the settling tank is also provided with a liquid filter.

The working principle of the aseptic inoculation device of the present embodiment is as follows:

The material enters the sedimentation tank through the feed port. After the supernatant liquid and the precipitated protein are layered, the height of the nozzle of the sampling tube in the sedimentation tank is adjusted through the sliding between the sampling tube and the casing, so that the nozzle is at the Between the interface between the supernatant and the precipitated protein, the gas source is fed into the gas to pressurize, and the supernatant is discharged through the hygienic diaphragm valve of the sampling tube under the action of air pressure. Feed tube to collect precipitated protein.

All documents mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. In addition, it should be understood that after reading the above teaching content of the utility model, those skilled in the art can make various changes or modifications to the utility model, and these equivalent forms also fall within the scope defined by the appended claims of the application.

Claims (10)

1. a kind of albumen precipitation supernatant separation device, which is characterized in that the device includes settling tank (10), gas source (20), the gas source is connected by gas pipeline (30) with the settling tank；

The tank skin of the settling tank is equipped with feed pipe (11), discharge nozzle (12), supernatant separation device and form (13)；

The supernatant separation device includes casing (14) and probe tube (15), and the casing passes through the upper tank of the settling tank Wall, the probe tube pass through described sleeve pipe, between the probe tube and described sleeve pipe be tightly connected and can be free to slide, institute The lower ends bent of the probe tube in settling tank is stated at being parallel to the horizontal plane, the nozzle of the probe tube in the settling tank is equipped with 30- 60 ° are gone up scarves, and the nozzle of the probe tube outside settling tank is equipped with diaphragm valve (16).

2. device as described in claim 1, which is characterized in that the bottom of the settling tank is pyramidal structure, and described goes out The bottom of settling tank is arranged in expects pipe.

3. device as described in claim 1, which is characterized in that form longitudinal arrangement on the tank skin of settling tank.

4. device as described in claim 1, which is characterized in that the form is transparent window.

5. device as described in claim 1, which is characterized in that the probe tube and described sleeve pipe are stereoplasm tube.

6. device as described in claim 1, which is characterized in that the diaphragm valve is sanitary diaphragm valve.

7. device as described in claim 1, which is characterized in that be additionally provided with liquid filtering on the probe tube outside the settling tank Device (17).

8. device as described in claim 1, which is characterized in that the gas pipeline of crossing is additionally provided with air filter (31).

9. device as described in claim 1, which is characterized in that the feed pipe and discharge nozzle is equipped with valve (18).

10. device as described in claim 1, which is characterized in that the settling tank is additionally provided with heater.