CN209148584U - An Electrochemical Sensor Electrode Test Piece for Multi-parameter Detection - Google Patents

Abstract

The utility model discloses an electrochemical sensor electrode test piece for multi-parameter detection, which comprises a base plate and an electrode group. The utility model provides an electrochemical sensor electrode test piece which can perform multi-parameter detection and improve the utilization rate of the electrode test piece.

Description

An Electrochemical Sensor Electrode Test Piece for Multi-parameter Detection

technical field

The utility model relates to the field of electrochemistry, in particular to an electrochemical sensor electrode test piece for multi-parameter detection.

Background technique

Electrochemical test strips based on the principle of electrochemical biosensors are widely used in the field of point-of-care testing (POCT). Physiological fluids such as blood, urine, sweat, and saliva contain many test parameters of interest to clinical laboratories, such as glucose, cholesterol, glycosylated hemoglobin, blood ketones in blood, uric acid in urine, and lactic acid in sweat. And these detection parameters have frequent detection requirements. At present, most of the common commercial electrochemical test strips have only one reaction chamber, and each measurement can only detect a single component. A drop of blood or a drop of sample liquid to be tested can only detect one parameter.

SUMMARY OF THE INVENTION

The purpose of the utility model is to provide an electrochemical sensor electrode test piece that can perform multi-parameter detection and improve the utilization rate of the electrode test piece.

The technical scheme adopted by the utility model to solve the technical problem is as follows: an electrochemical sensor electrode test piece for multi-parameter detection, comprising a substrate and an electrode group, the electrode group at least includes a working electrode and an auxiliary electrode, and the opposite end surfaces of the substrate are respectively There is an electrode set.

As a preferred solution, one electrode group is provided on each side of the substrate.

As a preferred solution, an identification terminal is respectively provided on two opposite end surfaces and two side surfaces of the substrate, and the identification terminal is located between the working electrode and the auxiliary electrode.

As a preferred solution, each electrode group also has a reference electrode. The working electrode and the auxiliary electrode form a detection loop, which generates a current capture electrical signal and then converts it into the concentration of the sample to be tested. The working electrode and the reference electrode constitute a system that is not connected or basically less energized, and the electrode potential of the working electrode is measured by using the stability of the reference electrode potential. Preferably, the electrode group further includes detection electrodes. The detection electrode has a short-circuit self-test function or a lack of sample addition detection function. If the detection electrode has a short-circuit self-test function, that is, when the test strip is inserted into the detector, if the test strip has been used or is short-circuited, it will be judged as a short circuit and cannot be continued. use. If the detection electrode has the function of insufficient sample addition detection, when the sample to be tested is insufficient to be added to the test strip, the instrument will display that the sample added amount is insufficient. The electrode test piece of the electrochemical sensor can be equipped with different numbers of electrodes according to different requirements of electrochemical action.

As a preferred solution, the electrode group has electrode terminals, conductive parts, and reaction parts, and the electrode terminals, conductive parts, and reaction parts are connected in sequence.

As a preferred solution, the reaction parts of the working electrode and the auxiliary electrode are bent toward each other.

As a preferred solution, detection grooves are provided on both sides of the substrate, and the reaction part is located in the detection groove and exposed to the detection groove.

As a preferred solution, siphon cover plates are provided on both end surfaces and both sides of the base plate.

The advantages of the present utility model are:

1. By setting electrode groups on the front and back of the substrate, the working electrodes in the electrode group can modify different biochemical reagents to detect different samples to be tested, or can perform multi-parameter detection at the same time, without the need to repeatedly collect samples, increasing the testing time. Diversity.

2. Electrode groups are arranged on both sides of the substrate, and different samples to be tested can be detected on both sides of the substrate, which increases the variety of tests.

3. The four end faces of the substrate can detect samples to be tested, and can detect four different samples to be tested at the same time, or to detect four parameters of one sample to be tested.

4. For the same sample, the front and back and both sides of the electrode test piece can be quickly detected to obtain the test results, which is convenient for comparative analysis of test data, effectively reduces the complexity of the test, and improves the accuracy of the test.

5. The electrochemical electrode test piece is made into a multi-parameter detection electrode test piece with a multi-faceted structure, which improves the utilization rate of the electrode test piece and reduces the production cost of the electrode test piece.

Description of drawings

Figure 1 is a schematic structural diagram of the utility model.

FIG. 2 is a front view of the electrode test piece.

FIG. 3 is a cross-sectional view taken along the line A-A in FIG. 2 .

Marked in the figure: substrate 1, detection slot 11, working electrode 21, auxiliary electrode 22, electrode terminal 212, conduction part 213, reaction part 211, reference electrode 23, identification terminal 24, siphon cover plate 25.

Detailed ways

The present utility model will be further described below with reference to the accompanying drawings and specific embodiments.

Example 1

As shown in FIG. 1 and FIG. 2 , an electrochemical sensor electrode test piece for multi-parameter detection includes a substrate 1 and an electrode group. The electrode group at least includes a working electrode 21 and an auxiliary electrode 22 , and the electrode group is located on two opposite sides of the substrate 1 . end face. The working electrode 21 and the auxiliary electrode 22 form a detection loop, which generates a current capturing electrical signal and then converts it into the concentration of the sample to be tested. The two opposite end surfaces refer to the front and back surfaces of the substrate 1 . For the positive and negative working electrode strips, different biochemical reagents can be modified to test different samples, which increases the diversity of the test. There is no need to repeatedly collect samples, and a dual-parameter detection of samples is realized.

As shown in FIG. 1 , the two opposite end surfaces of the substrate 1 refer to the front surface and the back surface of the substrate 1 , and one electrode group is provided on the front surface and the back surface respectively. The working electrode 21 and the auxiliary electrode 22 form a detection loop, which generates a current capturing electrical signal and then converts it into the concentration of the sample to be tested.

As shown in FIG. 1 , the electrode set further includes a reference electrode 23 . The working electrode 21 and the reference electrode 23 constitute a non-conductive or substantially less energized system, and the potential stability of the reference electrode 23 is used to measure the electrode potential of the working electrode. Preferably, the electrode group further includes detection electrodes. The detection electrode has a short-circuit self-test function or a lack of sample addition detection function. If the detection electrode has a short-circuit self-test function, that is, when the test strip is inserted into the detector, if the test strip has been used or is short-circuited, it will be judged as a short circuit and cannot be continued. use. If the detection electrode has the function of insufficient sample addition detection, when the sample to be tested is insufficient to be added to the test strip, the instrument will display that the sample addition amount is insufficient, and an additional sample amount must be added within the specified time for accurate testing. The electrode test piece of the electrochemical sensor can be equipped with different numbers of electrodes according to different requirements of electrochemical action.

As an example, the working electrodes 21 on both ends of the substrate 1 are drawn out by their respective wires, and the auxiliary electrodes 22 are connected in parallel. Each of the working electrodes 21 can constitute a detection circuit.

As a specific embodiment, two end faces of the substrate 1 are provided with identification terminals 24 , and the identification terminals 24 are located between the working electrode 21 and the auxiliary electrode 22 , as shown in FIG. 2 . Each of the end faces has one and only one identification terminal 24 which is electrically connected to an adjacent one of the electrode terminals, so as to achieve the effect of identification. For example, the identification terminal 24 is only electrically connected to the adjacent working electrode 21 .

As a specific embodiment, as shown in FIG. 2 , both end surfaces of the substrate 1 are provided with siphon cover plates 25 . The siphon cover plates 25 are adhered and covered on the two end surfaces of the base plate 1 respectively. The siphon cover plate 25 is made of insulating material pasted and covered with a hydrophilic film, and there is a gap in the middle of the insulating material to form a straight channel as a capillary diversion channel.

As an embodiment, as shown in FIG. 2 , the electrode group further includes a reference electrode 23 , and the working electrode 21 , the auxiliary electrode 22 and the reference electrode 23 are arranged in sequence. The electrode groups all have electrode terminals 212, conductive parts 213 and reaction parts 211, and the electrode terminals 212, conductive parts 213 and reaction parts 211 are connected in sequence, that is, the working electrode 21, the auxiliary electrode 22 and the reference electrode 23 all have electrode terminals 212, conductive parts 212, and a conductive part 211. part 213 and reaction part 211 . As a preferred solution, the reaction parts 211 of the working electrode 21 and the auxiliary electrode 22 are bent toward each other, which is convenient for detection.

As a specific example, the working electrode 21 , the auxiliary electrode 22 , the identification terminal 24 and the reference electrode 23 on the front side of the substrate 1 are arranged in opposite order to the working electrode 21 , the auxiliary electrode 22 , the identification terminal 23 and the reference electrode 23 on the reverse side. , so that both positive and negative connections can work normally.

The working electrode 21 can modify biochemical reagents. The biochemical reagent can be glucose oxidase, mutarotase, glucose dehydrogenase, lactate oxidase, uric acid oxidase, cholesterol oxidase, phospholipase, amino acid enzyme, horseradish peroxidase, β-hydroxybutyric acid Any one or several of enzymes such as dehydrogenase. The biochemical reagent is modified on the multi-faceted working electrode, the modified biochemical reagent reacts with the components in the sample to be tested, and the current is generated for analysis to obtain the composition of the sample to be tested, which is convenient and accurate; when the modified biochemical reagent is glucose When the modified biochemical reagent is lactate oxidase, the concentration of lactic acid in the sample can be detected, and when the modified biochemical reagent is urate oxidase, the concentration of uric acid in the sample can be detected .

When the modified biochemical reagents on the two working electrodes on the front and back are the same, and the biochemical reagent is glucose oxidase, the working principle is as follows:

(1) Take a drop of the test sample and inhale through the capillary channel to react with the biochemical reagents modified on the electrode.

(2) The frontal current detected by the electrochemical workstation is I ₁ .

(3) The reverse current detected by the electrochemical workstation is I ₂ .

(4) The current difference is made I=|I ₁ -I ₂ |, where the current difference I represents the correctness of the detection result, and the smaller the value, the higher the accuracy of the detection result. The concentration of glucose in the test sample can be calculated correspondingly.

When the modified biochemical reagents on the two working electrodes on the front and back are different, and the biochemical reagents are respectively glucose oxidase and uric acid oxidase, the working principle is as follows:

(1) Take a drop of the test sample and inhale through the capillary channel to react with the biochemical reagents modified on the electrode.

(2) The frontal current detected by the electrochemical workstation is I ₁ .

(3) The reverse current detected by the electrochemical workstation is I ₂ .

The concentration of glucose and uric acid in the test sample can be directly calculated by the values of I ₁ and I ₂ .

Example 2

The difference between this embodiment and Embodiment 1 is that an electrode group is provided on each of the two opposite sides of the substrate 1 . An electrode group is provided on both sides of the substrate 1, which can realize the detection of multiple samples or multiple parameters of the same sample. The two opposite sides of the substrate 1 refer to the left side and the right side of the substrate 1 .

As a specific embodiment, the electrode groups on two opposite sides of the substrate 1 are located in the detection groove 11 and have exposed surfaces that are in contact with the substance to be detected. Detection grooves 11 are provided on both sides, and the detection grooves 11 in the groove structure can prevent the sample to be tested from flowing out, see FIG. 3 . Specifically, one end of the detection tank 11 is provided with a sample addition port for adding the sample to be tested.

As a specific embodiment, identification terminals 24 are provided on both sides of the substrate 1 , and the identification terminals 24 are located between the working electrode 21 and the auxiliary electrode 22 . There is one and only one identification terminal 24 on the two sides, which is electrically connected to an adjacent electrode terminal, so as to achieve the effect of identification. For example, the identification terminal 24 is only electrically connected to the adjacent working electrode 21 .

As a specific example, the working electrodes 21, auxiliary electrodes 22, identification terminals 24, and reference electrodes 23 on both sides of the substrate 1 are arranged in the same order as the working electrodes 21, auxiliary electrodes 22, identification terminals 23, and reference electrodes 23 on the opposite side. The order of arrangement is reversed, so that both left and right connections can work normally.

As a specific embodiment, both sides of the substrate 1 are provided with siphon cover plates 25 .

As an example, the working electrodes 21 on the two ends and the two sides of the substrate 1 are drawn out by respective wires, and the auxiliary electrodes 22 are connected in parallel. The lead wire, the auxiliary electrode 22 and any one of the working electrodes 21 can form a detection circuit.

As a specific embodiment, the substrate 1 is made of plastic material, such as PBT plastic, the substrate 1 has a certain thickness, and the substrate 1 wraps the electrode group to meet the placement width of the electrode groups on both sides.

When the modified biochemical reagents on the front and back, left and right sides of the working electrodes have different components, four different parameters of the same sample to be tested or parameters of different samples to be tested can be detected correspondingly.

The content described in the embodiments of the present specification is only an enumeration of the realization forms of the concept of the utility model. The protection scope of the present invention should not be regarded as limited to the specific forms stated in the embodiments. The protection scope of the present invention also extends to Equivalent technical means that can be conceived by those skilled in the art according to the concept of the present invention.

Claims (8)

1. a kind of electrochemical sensor electrodes test piece of multi-parameter detection, which is characterized in that including substrate and electrode group, electrode group Including at least working electrode and auxiliary electrode, an electrode group is respectively provided in the opposite both ends of the surface of substrate.

2. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as described in claim 1, which is characterized in that substrate two Side is respectively provided with an electrode group.

3. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as claimed in claim 2, which is characterized in that work electricity Recognition terminal is equipped between pole and auxiliary electrode.

4. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as claimed in claim 3, which is characterized in that Mei Ge electricity Pole group also has reference electrode.

5. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as claimed in claim 4, which is characterized in that substrate two Working electrode, auxiliary electrode, recognition terminal and the reference electrode of end face put in order on the contrary；The work electricity of substrate two sides Pole, auxiliary electrode, recognition terminal and reference electricity put in order on the contrary.

6. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as described in claim 1, which is characterized in that electrode group With electrode terminal, conducting part and reacting part, electrode terminal, conducting part and reacting part are sequentially connected.

7. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as claimed in claim 6, which is characterized in that substrate two Side is respectively provided with detection slot, and reactive site is in detection slot and exposing to detection slot.

8. a kind of electrochemical sensor electrodes test piece of multi-parameter detection as claimed in claim 2, which is characterized in that substrate Both ends of the surface and two sides are equipped with siphon cover board.