CN209132220U - An ultra-high liquid chromatography separation system for the determination of pediatric therapeutic drugs - Google Patents

Abstract

A kind of measurement pediatric treatment drug superelevation liquid chromatographic separation system, including chromatographic column, connecting tube and thermostat, chromatographic column is fixed in thermostat and is connect respectively with pump and detector by connecting tube, the chromatographic column includes column tube, column tube both ends are fixed with the identical sealing structure of structure, sealing structure is by column cap nut, tapered sleeve, sieve plate, stud bolt and chock plug composition, column cap nut is socketed on column tube, tapered sleeve is socketed on the intracavitary column tube of column cap nut, sieve plate is plugged in the end of column tube and is fixed on column tube by stud bolt, stud bolt is threadedly coupled with column cap nut, sieve plate accommodating cavity and cone seal chamber are provided in the screw rod of the stud bolt, it is provided on the nut of stud bolt into sample output passage.The sieve plate fixation of the chromatographic column of the system is even closer, and increased tapered sleeve ensure that the compactness and reliability of connection, while the sealing structure disassembly of the chromatography column end is more easier, and is conveniently replaceable the sieve plate of pollution.

Description

An ultra-high liquid chromatography separation system for the determination of pediatric therapeutic drugs

technical field

The utility model relates to the field of medical detection instruments, in particular to an ultra-high liquid chromatography separation system for measuring pediatric therapeutic drugs.

Background technique

Therapeutic drug monitoring (TDM) is a new branch of clinical medicine developed in the field of drug therapy medicine in the past 20 years, and it is one of the important contents of clinical pharmacology research. It uses various sensitive modern analysis and detection methods to quantitatively analyze the concentration of drugs or metabolites in biological samples of patients, so as to explore the relationship between the blood concentration in the patient's body and the curative effect and toxic and side effects. The best individualized dosing regimen. TDM technology mainly includes chromatography technology and immunoassay technology. Chromatography techniques include high performance liquid chromatography, gas chromatography, liquid chromatography, mass spectrometry, etc.; immunoassay techniques include fluorescence polarization immunoassay, enzyme immunoassay, and radioimmunoassay. As an important field in modern medical pharmacy, TDM plays an extremely important role in improving patients' medication compliance, improving the safety and effectiveness of drug treatment, and reducing the occurrence of adverse drug reactions.

However, in the existing ultra-high liquid chromatography separation system for the determination of pediatric therapeutic drugs, especially the chromatographic column, the frit plate is easily displaced during the drug determination process, resulting in the leakage of the column tube and the contamination of the packing in the column tube. .

Utility model content

In order to solve the above problems, the utility model designs an ultra-high liquid chromatography separation system for the determination of pediatric therapeutic drugs. The sieve plate of the chromatographic column of the system is more tightly fixed, and the increased cone sleeve ensures the tightness and reliability of the connection. At the same time, the sealing structure at the end of the chromatographic column is easier to disassemble, and it is convenient to replace the polluted frit.

In order to realize the above-mentioned technical purpose and achieve the above-mentioned technical effect, the present invention is realized through the following technical solutions:

An ultra-high liquid chromatography separation system for measuring pediatric therapeutic drugs, comprising a chromatographic column, a connecting pipe and a thermostat, wherein the chromatographic column is fixed in the thermostat and is respectively connected with a pump and a detector through the connecting pipe, characterized in that the described The chromatographic column includes a column tube. The two ends of the column tube are fixed with a sealing structure with the same structure. The sealing structure consists of a column head nut, a cone sleeve, a sieve plate, a stud bolt and a plug head. Connected to the column tube in the stud nut cavity, the sieve plate is blocked at the end of the column tube and fixed on the column tube by the stud bolt, the stud bolt is threadedly connected with the stud nut, and the screw of the stud bolt is opened inside. There is a sieve plate accommodating cavity and a conical sealing cavity. The nut of the stud bolt is provided with a sample inlet and outlet channel. The inlet and outlet channels are connected to the sieve plate accommodating cavity. The conical sealing cavity of the stud bolt and the inner wall of the sample inlet and outlet channel are opened There are female threads, the outer wall of the cone sleeve is provided with male threads, the cone surface angle of the cone sleeve is smaller than the cone surface angle of the conical sealing cavity of the stud bolt, and the plug is inserted into the sample inlet and outlet channel of the stud bolt and is threadedly connected with the stud bolt.

Further, the column tube is marked with a flow direction arrow, and the column tube is made of stainless steel.

Further, the taper sleeve is sintered with nickel powder material, and the taper sleeve is provided with a through hole with a diameter of 1um or 2um.

The beneficial effects of the utility model are: the sieve plate of the chromatographic column of the system is more tightly fixed, the increased taper sleeve ensures the tightness and reliability of the connection, and the sealing structure at the end of the chromatographic column is easier to disassemble, and it is convenient to replace the pollution sieve plate.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings required for the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without creative effort.

Fig. 1 is the overall structure schematic diagram of described chromatographic column;

Fig. 2 is the structural representation of each component of the described chromatographic column;

Fig. 3 is the sectional structure schematic diagram of the sealing structure of the described chromatographic column;

FIG. 4 is a schematic diagram of the usage status of the chromatographic column.

In the accompanying drawings, the list of components represented by each number is as follows:

1-column tube, 2-column head nut, 3-column head bolt, 4-plug head, 5- frit plate, 6-cone sleeve, 7-connecting pipeline, 8-pipeline joint, 31- frit plate accommodating cavity , 32-conical seal cavity.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Referring to Figures 1-4, an ultra-high liquid chromatography separation system for the determination of pediatric therapeutic drugs, including a chromatographic column, a connecting pipe and a thermostat, the chromatographic column is fixed in the thermostat and is respectively connected to the pump and the detector through the connecting pipe , the chromatographic column includes a column tube 1, and the two ends of the column tube 1 are fixed with a sealing structure with the same structure. The nut 2 is sleeved on the column tube 1, the tapered sleeve 6 is sleeved on the column tube in the cavity of the column head nut 2, the sieve plate 5 is blocked at the end of the column tube 1 and is fixed to the column tube 1 by the stud bolt 3 On the top, the stud bolt 3 is threadedly connected with the stud nut 2, the screw of the stud bolt 3 is provided with a sieve plate accommodating cavity 31 and a conical sealing cavity 32, and the nut of the stud bolt 3 is provided with a sample inlet and outlet channel, The sample inlet and outlet channels communicate with the sieve plate accommodating cavity 31, the conical sealing chamber 32 of the stud bolt 3 and the inner wall of the sample inlet and outlet channels are provided with female threads, the outer wall of the tapered sleeve 6 is provided with male threads, and the tapered surface of the tapered sleeve 6 is provided with male threads. The angle is smaller than the cone surface angle of the conical sealing cavity 32 of the stud bolt 3 , and the plug 4 is inserted into the sample inlet and outlet channel of the stud bolt 3 and is threadedly connected with the stud bolt 3 .

Wherein, the column tube 1 is marked with a flow direction arrow, and the column tube 1 is made of stainless steel.

Wherein, the tapered sleeve 6 is sintered with nickel powder material, and the tapered sleeve 6 is provided with a through hole with a diameter of 1um or 2um.

A specific application of the device is: when using the chromatographic column of the separation system, unscrew the plug 4 from the stud bolt 3, and then screw the pipe joints 8 on the connecting pipeline 7 into the two ends of the column pipe 1 respectively. on the stud bolt 3; when connecting to the connecting pipeline 7, observe the flow arrow on the column tube 1 to make it point in the same direction as the flow direction of the eluent; when tightening the sealing structure at both ends of the column tube 1, remove the Push to the outside of the stud head, and tighten the stud bolt 3 and the stud nut 2 at the same time. When screwing the stud bolt 3, the taper sleeve 6 slides down into the conical sealing cavity 32 of the stud bolt 3 under the extrusion action of the stud nut 2. In contact with the female thread surface of the conical sealing cavity 32, since the front end of the conical sleeve 6 is convenient to be thin, it deforms under the extrusion of the conical sealing cavity 32 of the stud bolt 3 and holds the column pipe 1 tightly, and the The surface and the inner wall of the conical sealing cavity 32 form a ring-shaped sealing surface, that is, a line seal; the conical sleeve 6, which is formed by the deformation of metal under high pressure, is very tight and reliable, and can withstand very high pressure. At the same time of high pressure, it can also cause a very reliable mechanical connection, so that it can firmly hold the hooped column pipe or connecting pipe without slipping off; Column tube 1 port, so as to prevent the frit plate from being offset, resulting in liquid leakage at the end of the column tube and contamination of the packing.

In the description of this specification, description with reference to the terms "one embodiment", "example", "specific example", etc. means that a specific feature, structure, material or characteristic described in connection with the embodiment or example is included in the present invention. in at least one embodiment or example. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details, nor do they limit the invention to only the described embodiments. Obviously, many modifications and variations are possible in light of the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principle and practical application of the present invention, so that those skilled in the art can well understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims (3)

1. an ultra-high liquid chromatography separation system for measuring pediatric therapeutic drugs, comprising a chromatographic column, a connecting pipe and a thermostat, the chromatographic column is fixed in the thermostat and is connected with a pump and a detector respectively by the connecting pipe, it is characterized in that, The chromatographic column includes a column tube, and both ends of the column tube are fixed with a sealing structure with the same structure. The taper sleeve is sleeved on the column tube in the column head nut cavity, and the sieve plate is blocked at the end of the column tube and is fixed on the column tube by the column head bolt. A sieve plate accommodating cavity and a conical sealing cavity are opened inside, the nut of the stud bolt is provided with a sample inlet and outlet channel, and the sample inlet and outlet channel is connected with the sieve plate accommodating cavity, and the conical sealing cavity of the stud bolt and the inner wall of the sample inlet and outlet channel There is a female thread on the top, and a male thread is formed on the outer wall of the taper sleeve. The cone surface angle of the taper sleeve is smaller than the cone surface angle of the conical sealing cavity of the stud bolt. The plug is inserted into the inlet and outlet channels of the stud bolt and is threaded with the stud bolt. connect. 2. A kind of ultra-high liquid chromatography separation system for measuring pediatric therapeutic drugs according to claim 1, characterized in that, the column tube is marked with a flow direction arrow, and the column tube is made of stainless steel material. 3. a kind of ultra-high liquid chromatography separation system for measuring pediatric therapeutic drugs according to claim 1, is characterized in that, described taper sleeve adopts nickel powder material sintering to form, and the hole diameter that is opened on taper sleeve is 1um Or 2um round hole.