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CN208902743U - Sample processing device - Google Patents

Sample processing device Download PDF

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Publication number
CN208902743U
CN208902743U CN201821212593.8U CN201821212593U CN208902743U CN 208902743 U CN208902743 U CN 208902743U CN 201821212593 U CN201821212593 U CN 201821212593U CN 208902743 U CN208902743 U CN 208902743U
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China
Prior art keywords
microscope carrier
sample
working region
nucleic acid
processing device
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CN201821212593.8U
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Chinese (zh)
Inventor
曹玉峰
陈韡崧
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Shandong See Microbiology Technology Co Ltd
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Shandong See Microbiology Technology Co Ltd
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Priority to CN201821212593.8U priority Critical patent/CN208902743U/en
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Abstract

This disclosure relates to sample processing device, sample processing device includes microscope carrier transport establishment and multiple microscope carriers for being movably disposed in microscope carrier transport establishment, the working region arranged in microscope carrier transport establishment includes at least: the first working region, and first mechanical arm is for dispensing sample to each sample container of the microscope carrier in the first working region；It is provided with the second working region of nucleic acid extraction mechanism, nucleic acid extraction mechanism is configured to move along the direction close to or far from microscope carrier, for extracting nucleic acid contained in the sample in sample container and storing the nucleic acid of extraction to each sample container；Third working region, second mechanical arm detects nucleic acid for dispensing the nucleic acid of detection reagent and extraction into multiple deep-well plates, each microscope carrier by microscope carrier driving mechanism can along microscope carrier moving direction successively from the first working region move to second and third working region to execute relevant work.Thus sample processing device can improve sample process efficiency.

Description

Sample processing device
Technical field
This disclosure relates to sample process technical field, and in particular, to a kind of sample processing device and sample processing method.
Background technique
In existing sample processing device, it is each on fixed microscope carrier to being loaded into generally use movable working arm Sample container successively executes dispensing sample, extracts the processing operations such as nucleic acid, detection nucleic acid.And the sample process of this structure is set Standby not only volume is big and there is a problem of that shared arrangement space is larger, but also since movable working arm is directed to the load of different location The motion path of platform is also complex and causes to lead to the problem of sample process efficiency lower.
Utility model content
Purpose of this disclosure is to provide a kind of sample processing devices that can be improved sample process efficiency.
To achieve the goals above, according to one aspect of the disclosure, a kind of sample processing device is provided, the sample process Equipment includes microscope carrier transport establishment and multiple microscope carriers for being movably disposed in the microscope carrier transport establishment, each microscope carrier On be provided with multiple sample containers, useful multiple reagent solutions in processing sample are accommodated in each sample container, it is described It is disposed with multiple working regions in microscope carrier transport establishment, first mechanical arm and the second machinery are provided in the microscope carrier transport establishment Arm, the working region include at least: the first working region, and the first mechanical arm is positioned close to first working region Position, and the first mechanical arm is set as, can be along the longitudinal direction for being parallel to microscope carrier moving direction and perpendicular to the load The transverse direction of platform moving direction adjusts position, and can go up and down along short transverse, for dispensing sample to described first In each sample container of the microscope carrier in working region；Second working region is provided with core in second working region Sour extraction mechanism, which is configured to move along the direction close to or far from the microscope carrier, for extracting Nucleic acid contained in sample in sample container, and the nucleic acid of extraction is stored to each sample container；Third work Make region, the second mechanical arm is positioned close to the position of the third working region, and the second mechanical arm is set as, energy It is enough to adjust position along the longitudinal direction and the transverse direction, and can be gone up and down along the short transverse, to be used for multiple The nucleic acid of detection reagent and extraction is dispensed in deep-well plates respectively and detects nucleic acid, each microscope carrier passes through microscope carrier driving mechanism energy It is enough successively to move to second working region and the third work from first working region along the microscope carrier moving direction Make region, to execute relevant work.
According to another aspect of the present disclosure, a kind of sample process side using sample processing device as described above is provided Method, the sample processing method include: first step, and microscope carrier is moved to first working region along microscope carrier moving direction, benefit With dispensing sample in the first mechanical arm respectively each sample container on the microscope carrier；Second step, microscope carrier It is moved to second working region from first working region along the microscope carrier moving direction, utilizes the nucleic acid extraction machine Structure extracts nucleic acid contained in the sample in the sample container on microscope carrier, and the nucleic acid of extraction is stored to each sample Container；Third step, microscope carrier are moved to the third working region from second working region, mechanical using described second The nucleic acid stored in the sample container on the microscope carrier is dispensed in multiple deep-well plates that arm has detection reagent to dispensing and is detected Nucleic acid, on the microscope carrier moving direction from first working region to the third working region, adjacent every two Microscope carrier in microscope carrier positioned at front side is while executing any one step of the first step into third step, after being located at The microscope carrier of side executes the previous step of any one step.
Pass through technical solution as described above, that is, use in the sample processing device of the disclosure in microscope carrier transport establishment Move up the movable sample process mode of microscope carrier of dynamic load platform, and not in existing usually by mobile mechanical arm by the way of To microscope carrier execute respective sample processing operation the fixed sample process mode of microscope carrier, therefore, the disclosure using movable load The sample processing device of platform and the sample processing device phase under the existing middle stationary state using microscope carrier by mobile machine arm mode Smaller than, equipment volume and multiple microscope carriers are performed simultaneously corresponding operation in corresponding working region, to significantly improve sample Present treatment efficiency.
Other feature and advantage of the disclosure will the following detailed description will be given in the detailed implementation section.
Detailed description of the invention
Attached drawing is and to constitute part of specification for providing further understanding of the disclosure, with following tool Body embodiment is used to explain the disclosure together, but does not constitute the limitation to the disclosure.In the accompanying drawings:
Fig. 1 is the perspective view one according to the sample processing device of disclosure specific embodiment；
Fig. 2 is the perspective view two according to the sample processing device of disclosure specific embodiment；
Fig. 3 is the main view according to the sample processing device of disclosure specific embodiment；
Fig. 4 is the side view according to the sample processing device of disclosure specific embodiment；
Fig. 5 is the cross-sectional view in Fig. 4 along line A-A cutting；
Fig. 6 is the cross-sectional view in Fig. 4 along line B-B cutting；
Fig. 7 is the top view according to the sample processing device of disclosure specific embodiment；
Fig. 8 is the cross-sectional view for rotating 180 ° in Fig. 7 after line C-C cutting, each in figure in order to be readily appreciated Structure；
Fig. 9 is the cross-sectional view in Fig. 7 along line D-D cutting；
Figure 10 is each in upper layer microscope carrier shipping platform according to removing in the sample processing device of disclosure specific embodiment The top view of a consumptive material, here, the consumptive material removed includes deep-well plates, sampling hammerhead, probe tube, dispensing pipette tips and detection reagent Accommodate pipe；
Figure 11 is the cross-sectional view in Figure 10 along E-E line；
Figure 12 is the perspective view according to the nucleic acid extraction mechanism of disclosure specific embodiment；
Figure 13 is the pipette tips mobile device for being provided with pipette tips and ejecting releasing mechanism according to disclosure specific embodiment Main view；
Figure 14 is the right view of Figure 13；
Figure 15 is the rearview of Figure 13；
Figure 16 is the main view that releasing mechanism is ejected according to the pipette tips of disclosure specific embodiment；
Figure 17 is the right view of Figure 16；
Figure 18 is the cutaway view Amplified image in Figure 17 along F-F line cutting；
Figure 19 is that the perspective view that sampling gun is removed in releasing mechanism is ejected according to the pipette tips of disclosure specific embodiment；
Figure 20 is the perspective view one according to the magnetic bead transfer device of disclosure specific embodiment；
Figure 21 is the perspective view two according to the magnetic bead transfer device of disclosure specific embodiment；
Figure 22 is the perspective view according to the cooperation assembly of the microscope carrier and microscope carrier transport establishment of disclosure specific embodiment；
Figure 23 is to be supported according to omission in the microscope carrier of disclosure specific embodiment and the cooperation assembly of microscope carrier transport establishment The perspective view of frame etc.；
Figure 24 is the side view of Figure 23；
Figure 25 is to reduce figure along the section view of G-G line cutting in Figure 24；
Figure 26 is the cutaway view Amplified image in Figure 25 along I-I line cutting；
Figure 27 is the cutaway view Amplified image in Figure 25 along J-J cutting；
Figure 28 is the perspective view according to the microscope carrier driven in translation structure of disclosure specific embodiment；
Figure 29 is the main view of Figure 28；
Figure 30 is the cutaway view Amplified image in Figure 29 along K-K line cutting；
Figure 31 is the perspective view according to the movable trolley of disclosure specific embodiment；
Figure 32 is the perspective view one according to the microscope carrier of disclosure specific embodiment；
Figure 33 is the perspective view two according to the microscope carrier of disclosure specific embodiment；
Figure 34 is the top view according to the microscope carrier of disclosure specific embodiment；
Figure 35 is the cutaway view Amplified image in Figure 34 along L-L line cutting；
Figure 36 is the bottom view according to the microscope carrier of disclosure specific embodiment；
Figure 37 is the structure chart of the first pulley and first pulley mounting base in Figure 36；
Figure 38 is the cross-sectional view in Figure 37 along S-S line cutting；
Figure 39 is the perspective view one according to fixing seat in the microscope carrier of disclosure specific embodiment；
Figure 40 is the perspective view two according to fixing seat in the microscope carrier of disclosure specific embodiment；
Figure 41 is the perspective view one according to sliding seat in the microscope carrier of disclosure specific embodiment；
Figure 42 is the perspective view two according to sliding seat in the microscope carrier of disclosure specific embodiment；
Figure 43 is the step flow diagram according to the method for extracting nucleic acid of disclosure specific embodiment；
Figure 44 is the step flow diagram according to the sample processing method of disclosure specific embodiment.
Description of symbols
1- microscope carrier transport establishment, 11- microscope carrier shipping platform, the upper layer 111- microscope carrier shipping platform, the transport of 112- lower layer microscope carrier Platform, 12- microscope carrier driven in translation structure, the first driving motor of 121-, 122- activity trolley, the first transmission belt of 123-, 124- One driving wheel, the first driven wheel of 125-, 126- trolley guide rail, 127- second pulley component, 128-second pulley mounting bases, 13- microscope carrier goes up and down driving structure, and 131- goes up and down fixed pedestal, 132- lift cylinders, and 133- goes up and down fixed pedestal guide rail, 14- microscope carrier Pick and place opening, the second positioning seat of 15-, the first magnet plunger of 151-, 16- groove profile photoelectrical position sensor, 17- microscope carrier guide rail, 171- Microscope carrier mating groove, 172- microscope carrier retention bead, 18- microscope carrier return driving structure, the second driving motor of 181-, 182- second are passed Dynamic band, the second driving wheel of 183-, the second driven wheel of 184-, 185- transmission shaft, 19- braced frame,
2- microscope carrier, 21- locking structure, the first positioning seat of 22-, 221- locating slot, 222- block, 23- first pulley component, 231- first pulley mounting base, the fixed pulley of 232- first, the first movable pulley of 233-, 2331- wheel shaft, the first activity of 2332- Pulley bearings, 234- first pulley install block hole, 235- screw thread, 236- fastening nut, 237- microscope carrier guide rail mating groove, 238- Fastening nut mounting groove, 24- fixing seat, 241- fixing seat bottom plate, 242- fixing seat side wall, 243- sliding seat accommodating chamber, 244- Anti-detachment protrusion, 25- sliding seat, 251- container holding tank, 252- mistake proofing assembling structure, 253- sliding seat pedestal, 254- sliding seat Top plate, 26- sliding seat guidance set, 261- guide groove, 262- guide protrusions, 27- magnetic absorption member, 28- electromagnet latch, 29- Internal standard holding tank,
3- sample container,
4- nucleic acid extraction mechanism, 41- pipette tips mobile device, 411- pedestal, 412- releasing mechanism driving motor, 413- are detached from Mechanism screw-and-nut mechanism, 414- releasing mechanism guide frame, 415- fixed pedestal position sensor, the transfer of 42- magnetic bead Device, 421- magnetic bead transfer device fixing seat, the mobile panel guide of 4211-, 422- magnetic part movable plate, 423- magnet, 424- are led To long hole, 425- positioning screw, 426- magnetic part driving motor, 427- magnetic part screw rod, 428- magnetic part nut, 429- magnetism Part movable plate position sensor,
5- pipette tips eject releasing mechanism, 51- sampling gun, 511- telescopic rod, 512- main body, 513- telescopic rod channel, 514- Pipette tips interconnecting piece, 515- pipette tips linkage section, 516- seal section, 517- locking protrusion, 52- sampling gun actuator, the fixed base of 521- Seat, 5211- connecting rod mounting hole, 5212- axle sleeve, 5211- assembling stand, 522- sampling gun driving motor, the first screw rod of 523-, 524 first nuts, 525- driving baffle, 526- holding tank, 527- vertical plate, 528- upper water plate, 5281- guide rod, 5282- guideway, 5283- drive baffle position sensor, 529- lower horizontal plate, 5291- main body holding tank, 53- ejection Release piece, 531- eject disengaging plate, and 532- pipette tips interconnecting piece is inserted into hole, and 533- supports plate, 5331- telescopic rod holding tank, 534- Connecting rod, 535- reset spring,
6- first mechanical arm, 7- second mechanical arm, 8- stretching structure, the second magnet plunger of 81-, 82- electromagnet,
100- deep-well plates, 200- sampling hammerhead, 300- probe tube, 400- dispense pipette tips, and 500- detection reagent accommodates pipe,
The first working region A-, the second working region B-, C- third working region, the initial storage area of D- microscope carrier, E- One consumptive material storage area, E1- sampling hammerhead storage area, E2- probe tube storage area, the second consumptive material of F- storage area, F1- points Pipette tips storage area, F2- deep-well plates storage area are infused, F3- detection reagent accommodates pipe storage area,
Z1- microscope carrier moving direction, Z2- nucleic acid extraction direction, X1- longitudinal direction, X2- transverse direction, H- short transverse, P- First telescopic direction,
S1- first step, S11- internal standard dispense step, and S12- sample dispenses step, S2- second step, S21- cracking step Suddenly, S22- washing step, S23- elution step, S24- nucleic acid extraction step, S25- protein digestibility step, S3- third step, S31- detection reagent dispenses step, and S32- nucleic acid dispenses step, S4- microscope carrier return step.
Specific embodiment
It is described in detail below in conjunction with specific embodiment of the attached drawing to the disclosure.It should be understood that this place is retouched The specific embodiment stated is only used for describing and explaining the disclosure, is not limited to the disclosure.
In the disclosure, in the absence of explanation to the contrary, the noun of locality used such as " upper and lower, left and right " typically refers to " upper and lower, left and right " of corresponding component under use state, " inside and outside " refer to " inside and outside " for corresponding component outer profile, This, the mode of microscope carrier 2 is driven to clearly illustrate microscope carrier driven in translation structure 12 in the second working region B, mobile with microscope carrier On the basis of the Z1 of direction, it is defined as rear side positioned at the upstream side microscope carrier moving direction Z1, is located at the downstream side microscope carrier moving direction Z1 and defines For front side, the first working region A and third working region C are located at rear side and the front side of the second working region B.In addition, this " the microscope carrier moving direction " referred in open typically refers to substantially successively move from the first working region A on the basis of each microscope carrier It to the second working region B, third working region C and is finally repositioned to the direction of the initial storage area D of microscope carrier, as hollow closes Circumferential direction, can refer to the direction Z1 as shown in figure 11 herein, and " the nucleic acid extraction direction " referred in the disclosure usually exists The direction of motion of microscope carrier is directed to during second working region B, that is, nucleic acid extraction, nucleic acid extraction direction can be and above-mentioned load Platform moving direction it is identical or can in contrast to above-mentioned microscope carrier moving direction, here, in the preferred embodiment of the disclosure with Nucleic acid extraction has carried out in detail sample processing device and sample processing method in the direction opposite the example of microscope carrier moving direction Ground explanation, i.e., can refer to the direction Z2 as shown in Fig. 5 and Figure 11 in the nucleic acid extraction direction in following preferred embodiments.
Firstly, present disclose provides a kind of sample processing device and sample processing method, the sample processing device of the disclosure It can be used for detecting the nucleic acid for representing life entity hereditary feature basic unit with method, for example, the sample processing device of the disclosure It can be applied to the detection and diagnosis of the infectious diseases such as hepatitis, AIDS, influenza, hand-foot-and-mouth disease with sample processing method, can also use In detecting and diagnosing Other diseases, can be automated by the sample processing device and sample processing method of the disclosure such as flowering structure Execute sample automatic filling, nucleic acid extraction and detection nucleic acid equal samples and handle operation, realize sample process process it is complete from Dynamicization operation.
Here, as shown in Figure 1 to 11, the sample processing device of the disclosure may include microscope carrier transport establishment 1 and removable Multiple microscope carriers 2 in microscope carrier transport establishment 1 are set dynamicly, multiple sample containers 3, each sample are provided on each microscope carrier 2 Useful multiple reagent solutions in processing sample are accommodated in this container 3, are disposed with multiple workspaces in microscope carrier transport establishment 1 Domain is provided with first mechanical arm 6 and second mechanical arm 7 in microscope carrier transport establishment 1, and the working region includes at least: the first work Make region A, first mechanical arm 6 is positioned close to the position of the first working region A, and the first mechanical arm 6 is set as, Neng Gouyan It is parallel to the longitudinal direction X1 of microscope carrier moving direction Z1 and adjusts position perpendicular to the transverse direction X2 of microscope carrier moving direction Z1, And can be gone up and down along height direction H, for dispensing sample to each sample receiving of the microscope carrier 2 in the first working region A In device 3；It is provided with nucleic acid extraction mechanism 4 in second working region B, second working region B, the nucleic acid extraction mechanism 4 setting For that can be moved along the direction close to or far from microscope carrier 2, with for extracting nucleic acid contained in the sample in sample container 3, And the nucleic acid of extraction is stored to each sample container 3；Third working region C, second mechanical arm 7 are positioned close to third work Make the position of region C, and the second mechanical arm 7 is set as, can be adjusted along the longitudinal direction X1 and transverse direction X2 Position, and can be gone up and down along the height direction H, for dispensing detection reagent and extraction respectively into multiple deep-well plates 100 Nucleic acid and detect nucleic acid, each microscope carrier 2 by microscope carrier driving mechanism can along microscope carrier moving direction Z1 successively from first work Region A moves to the second working region B and third working region C, to execute relevant work.That is, the sample process of the disclosure is set The movable sample process mode of microscope carrier for moving up dynamic load platform 2 in microscope carrier transport establishment 1 is used in standby, and is not used existing In usually by way of mobile mechanical arm to microscope carrier execute respective sample handle operation the fixed sample process mode of microscope carrier, Therefore, the sample processing device using movable microscope carrier of the disclosure passes through moving machine under microscope carrier stationary state with existing middle use The sample processing device of tool arm mode is compared, and equipment volume is small and multiple microscope carriers 2 are held simultaneously in corresponding working region The corresponding operation of row, to significantly improve sample process efficiency.
Specifically, sample processing device as described above has the following course of work.That is, passing through microscope carrier driving mechanism Driving, microscope carrier 2 is moved to the first working region A along microscope carrier moving direction Z1, using first mechanical arm 6 respectively on microscope carrier 2 Sample is dispensed in each sample container 3.Dispensing has the microscope carrier 2 of sample from the first working region in sample container 3 later A is moved to the second working region B along microscope carrier moving direction Z1, extracts the sample container on microscope carrier 2 using nucleic acid extraction mechanism 4 Nucleic acid contained in sample in 3, and the nucleic acid of extraction is stored to each sample container 3.Here, magnetic bead can be used The modes such as method, centrifugal column method execute nucleic acid extraction operation, and nucleic acid extraction mechanism 4 can be extracted according to actual nucleic acid and used Mode carry out reasonable design into a variety of arrangements, such as using paramagnetic particle method nucleic acid extraction mode, nucleic acid is mentioned Taking mechanism 4 can correspondingly include magnetic force generation device and the sample for executing the sample transfer tasks for sample container 3 This transfer device etc..After completing nucleic acid extraction, the microscope carrier 2 of nucleic acid is placed with from the second workspace in 3 memory of sample container Domain B is moved to third working region C, dispenses in the multiple deep-well plates 100 for having detection reagent to dispensing using second mechanical arm 7 The nucleic acid stored in sample container 3 on microscope carrier 2 and detect nucleic acid.Multiple microscope carriers 2 can be in corresponding work as a result, Corresponding work is performed simultaneously in region, thus, it is possible to significantly improve the sample process operating efficiency entirely recycled.
The movement of microscope carrier 2 in sample processing device as described above can be by carrying the setting of microscope carrier driving mechanism It, can also be using the cooperation reality by microscope carrier driving mechanism and microscope carrier 2 on platform 2 in a manner of the active drive that realization voluntarily controls The passive matrix mode now passively controlled, the disclosure are not particularly limited the structure of microscope carrier driving mechanism, as long as can be real The now function that driving microscope carrier 2 moves in microscope carrier transport establishment 1 along microscope carrier moving direction Z1.In the disclosure, for letter Change stage structure in order to movement, and in order to enable the drive control mode of microscope carrier 2 is more simplified, sample processing device with The passive matrix mode cooperated using microscope carrier driving mechanism and microscope carrier is described in aftermentioned as specific embodiment.
With reference first to Figure 12 to Figure 21 to can be suitable for the nucleic acid extraction mechanism sample processing device as described above Structure, feature and function and effect be described.
As shown in Figure 12 to Figure 15, the nucleic acid extraction mechanism of the disclosure utilizes the sample with multiple sample holding tanks to accommodate Device 3 extracts nucleic acid, and the nucleic acid extraction mechanism 4 is arranged in microscope carrier transport establishment 1, and includes: pipette tips mobile device 41, pipette tips Mobile device 41 can be arranged in up and down in microscope carrier transport establishment 1 along height direction H, rifle is separatably arranged in for driving Sampling hammerhead 200 on head moving device 41 enables to sampling hammerhead 200 to adsorb sample along height direction H shift position The liquid in liquid or discharge sampling hammerhead 200 in container 3；Magnetic bead transfer device 42, the magnetic bead transfer device 42 include Magnetic part, magnetic part can be telescopically arranged in microscope carrier transport establishment 1 along the direction close to or far from sampling hammerhead 200, with So that magnetic part, which has, acts on sampling hammerhead 200 for magnetic state and removing to the magnetic bead offer magnetic force in sampling hammerhead 200 The demagnetizing state of the magnetic force of interior magnetic bead.As described above, the nucleic acid extraction mechanism of the disclosure extracts nucleic acid using paramagnetic particle method, specifically Ground, during extracting nucleic acid, the nucleic acid for be released after cell cracking to sample first is mixed with magnetic bead, is formed Nucleic acid-bead complexes, at this time can be by the decline of pipette tips mobile device 41 so that being loaded in pipette tips mobile device 41 Sampling hammerhead 200 adsorbs the bead complexes, passes through the lifting action of pipette tips mobile device 41 and magnetic bead transfer dress later 42 expanding-contracting actions towards the direction close to or far from sampling hammerhead 200 are set, which is transferred to sample respectively and is held It receives in each sample holding tank of device 3 and executes and the operations such as correspondingly wash, elute.Specifically, in multiple sample holding tanks Reaction reagent (for example, cell pyrolysis liquid, cleaning solution, eluent etc.) between transfer nucleic acid-bead complexes when, nucleic acid-magnetic Pearl compound is placed in always in sampling hammerhead 200, and nucleic acid-magnetic bead is compound in the case where the offer of magnetic bead transfer device 42 is for magnetic state Object is adsorbed on the inner wall of sampling hammerhead 200, in this case by sampling hammerhead 200 waste liquid (such as complete cracking cracking Waste liquid, the scrub raffinate for completing washing, the elution waste liquid for completing elution etc.) it is expelled to the corresponding sample appearance of sample container 3 It receives in slot, sampling hammerhead 200 holds next sample that internal nucleic acid-bead complexes are expelled to sample container 3 later It receives in slot.And in existing sample extraction equipment, generally use mechanical arm moves in microscope carrier transport establishment, and microscope carrier and sample The container nucleic acid extraction mode fixed relative to microscope carrier transport establishment, in the case, during nucleic acid extraction, pass through by Bead complexes are placed in always in the state of sample container, and waste liquid and injection in sample container are drawn by sampling hammerhead The mode of new reaction reagent executes nucleic acid extraction operation.Specifically, it is accommodated in existing in nucleic acid-bead complexes and sample After a certain reaction reagent in device is sufficiently mixed, magnetic force generation device provides magnetic force to sample container, so that nucleic acid-magnetic bead is multiple It closes object to be adsorbed on sample container inner wall, at this point, mechanical arm driving sampling hammerhead draws the reaction reagent in sample container Waste liquid and exclude, while the sampling hammerhead that discarded use is crossed, later mechanical arm reload new sampling hammerhead by another Reaction reagent is injected into sample container and is mixed with nucleic acid-bead complexes, makees to successively execute nucleic acid extraction Industry.This sample processing device in existing is during nucleic acid extraction, since a reaction reagent is corresponding using a sampling Pipette tips lead to the serious waste of sampling hammerhead, and hold each sample often through the mode of mobile magnetic force generation device Device of receiving is executed for magnetic or degaussing operation, or respectively accordingly arrangement magnetic force generates dress in the position close to each sample container It sets, so that the nucleic acid extraction operation of sample processing device is increasingly complex, and there is the problem of manufacture and processing cost valuableness. And for the nucleic acid extraction mechanism and sample processing device of the disclosure, nucleic acid extraction mechanism 4 passes through in microscope carrier transport establishment 1 Pipette tips mobile device 41 and magnetic bead transfer device 42 are set, wherein the magnetic part of magnetic bead transfer device 42 is in microscope carrier transport establishment 1 On be set as, can be flexible along the direction close to or far from the sampling hammerhead 200, thus during nucleic acid extraction, in sample Receiving is useful in the state of each reaction reagent for extracting nucleic acid in each sample holding tank of this container 3, corresponding Bead complexes containing sample and magnetic bead after reacting in sample holding tank follow sampling hammerhead 200 mobile always, therefore The nucleic acid extraction of each sample only needs to can be realized as by a sampling hammerhead 200, thereby saves sampling hammerhead consumptive material, Nucleic acid extraction cost is reduced, and due to executing core in the state of accommodating each reaction reagent in each sample holding tank Acid extracts operation, after thus reacting in the sampling hammerhead 200 correspondence sample holding tanks that need to shift sample container 3 Solution, without being executed again such as the existing middle movement for injecting corresponding reaction reagent into each holding tank, thus whole Improve nucleic acid extraction operating efficiency.In addition, during nucleic acid extraction, due to 41 drive sampling guns of pipette tips mobile device First 200 along height direction H shift position, therefore magnetic bead transfer device 42 can be made relatively fixed in microscope carrier transport establishment 1 In the state of, it is convenient for providing magnetic force to nucleic acid-bead complexes by the expanding-contracting action of the magnetic part of magnetic bead transfer device 42 Or removal magnetic force, so that nucleic acid extraction simple operation, reduces the system of nucleic acid extraction mechanism and sample processing device It makes and processing cost.
Here, the arrangement of various reasonable, example can be used for pipette tips mobile device 41 and magnetic bead transfer device 42 Such as, optionally, pipette tips mobile device 41 includes the pedestal 411 being arranged in microscope carrier transport establishment 1 and can be along the short transverse The ejection releasing mechanism 5 of the pipette tips on pedestal 411 is arranged in H up and down.Here, can be suitable for the rifle pipette tips mobile device 41 The crown goes out releasing mechanism 5 and is configured to such as flowering structure, that is, as shown in Figure 16 to Figure 19, pipette tips ejection releasing mechanism 5 be can wrap Sampling gun 51, sampling gun actuator 52 and the ejection release piece 53 for installing sampling hammerhead 200 are included, sampling gun actuator 52 Driving portion connect with the telescopic rod 511 of sampling gun 51 and for driving telescopic rod 511 flexible, so that sampling gun 51 is sampling gun First 200 provide the pressure of the suction of imbibition or drain, and ejection release piece 53 is mounted on sampling gun actuator 52 and enables to Sampling hammerhead 200 is separated with sampling gun 51.As described above, in the case where sampling hammerhead 200 is loaded into the use state of sampling gun 51, By the driving of the driving portion of sampling gun actuator 52, sampling gun 51 carries out expanding-contracting action and makes 200 liquid draw of sampling hammerhead Body or discharge liquid, pipette tips 200 to be sampled can make sampling gun by ejecting release piece 53 after completing nucleic acid extraction operation First 200 easily fall out from sampling gun 51, improve the efficiency of loading and unloading of sampling hammerhead 200, thus, it is possible to nucleic acid is effectively ensured to mention It is taken as the reliability of industry.
In addition, here, ejection release piece 53 can be set to the structure of various reasonable, as long as can be by sampling hammerhead 200 apply the active force towards off-direction, realize the function that sampling hammerhead 200 is detached from from sampling gun 51.For example, ejection Release piece 53 can be set into mobilizable clamping limb, clamp sampling hammerhead 200 by the clamping limb and by sampling hammerhead 200 are detached from from sampling gun 51, and for another example, optionally, as shown in fig. 16 and 18, sampling gun 51 includes main body 512 and multiple flexible Bar 511, main body 512 are arranged on the fixed pedestal 521 of sampling gun actuator 52, and have the both ends being inserted into for telescopic rod 511 Multiple telescopic rod channels 513 of opening, the top of each telescopic rod 511 are connect with the driving portion of sampling gun actuator 52, and each A telescopic rod 511 can telescopically be inserted into corresponding telescopic rod channel by the driving of driving portion along height direction H In 513, the bottom corresponding to each telescopic rod channel 513 of main body 512 be respectively structured as with corresponding sampling hammerhead 200 The pipette tips interconnecting piece 514 of connection, ejection release piece 53 is configured to move relative to each pipette tips interconnecting piece 514, with can So that each sampling hammerhead 200 is separated with corresponding pipette tips interconnecting piece 514.Wherein, it is formed in main body 512 multiple flexible Bar channel 513, here, each telescopic rod channel 513 is with the close extension end of corresponding telescopic rod 511 (in height under use state Spend direction H on top) part between for sealing cooperation, it can realize telescopic rod channel by way of arranging sealing ring Sealing cooperation between 513 and telescopic rod 511, with when telescopic rod 511 does extending action in telescopic rod channel 513, due to Area of low pressure is formed in telescopic rod channel 513, liquid can pass through the liquid sucting port of sampling hammerhead 200 under external atmosphere pressure effect It enters in sampling hammerhead 200.The working principle of this sampling gun 51 is similar with the working principle of syringe.In addition, pipette tips connect Socket part 514 can be formed as the arrangement of various reasonable, for example, to may be integrally formed at main body 512 right for pipette tips interconnecting piece 514 Should be in the bottom in telescopic rod channel 513, alternatively, for the ease of the structure of machine-shaping sampling gun 51, pipette tips interconnecting piece 514 It can be formed and be independent component and be connected to the bottom that main body 512 corresponds to telescopic rod channel 513.Pass through structure as described above Pipette tips eject releasing mechanism 5, it can by multiple sampling hammerheads 200 with the sample container of multiple groups sample holding tank 3 cooperation, the operations such as synchronous nucleic acid extraction for executing multiple samples, and make multiple sampling hammerheads by ejecting release piece 53 200 disposably fall off from each pipette tips interconnecting piece 514 simultaneously, and the dismounting for thus effectively saving sampling hammerhead 200 is taken Between, nucleic acid extraction efficiency equal samples treatment effeciency can be significantly improved.
Optionally, as shown in Figure 17 and Figure 19, ejection release piece 53 includes that can support up and down along the height direction H Ejection disengaging plate 531 on the fixed pedestal 521 of sampling gun actuator 52, the ejection disengaging plate 531 is in the height direction H The upper lower section positioned at main body 512, and eject and be formed with multiple pipette tips interconnecting piece insertions hole 532, each pipette tips on disengaging plate 531 Respectively accordingly perforation pipette tips interconnecting piece is inserted into hole 532 and is exposed to ejection disengaging plate the pipette tips linkage section 515 of interconnecting piece 514 The driving portion of 531 side, sampling gun actuator 52 is set as, and under the retracted mode that telescopic rod 511 retracts, can drive top Disengaging plate 531 squeezes the sampling hammerhead 200 on each pipette tips interconnecting piece 514 along the off-direction of sampling hammerhead 200 out.That is, being Sampling hammerhead 200 more efficiently is unloaded in the state of guaranteeing that sampling hammerhead 200 empties liquid, ejects disengaging plate 531 Make motion path smallizationer positioned at the position of neighbouring sampling hammerhead 200 and act rapidly on sampling hammerhead 200, specifically Ground, sampling hammerhead 200 is mounted on the part for being exposed to the side of ejection disengaging plate 531, in the drive of sampling gun actuator 52 In the state that dynamic portion's driving telescopic rod 511 is fully retracted, ejection disengaging plate 531 can further be driven to move along off-direction, So that ejection disengaging plate 531 and each sampling hammerhead 200 are in contact and push each sampling hammerhead 200 along off-direction, by This each sampling hammerhead 200 can fall off from each pipette tips interconnecting piece 514 simultaneously.It is enabled to by structure as described above The integral arrangement of pipette tips ejection releasing mechanism 5 is more rationalized.In addition, due to being utilized for driving stretching for sampling gun 51 The driving source that the flexible sampling gun actuator 52 of contracting bar 511 is moved as driving ejection disengaging plate 531, there is no need to independent again Independent driving source of the arrangement for driving ejection disengaging plate 531 to move, saves manufacturing cost and can satisfy so that pipette tips top The requirement of overall structure simplerization of releasing mechanism 5 out.
Here, in order to guarantee that the driving portion of sampling gun actuator 52 steadily and reliably drives ejection disengaging plate 531 to move And be in contact with each sampling hammerhead 200, and make pipette tips ejection releasing mechanism 5 each structure arrangement it is more compact and Rationally, optionally, as shown in figure 19, the ejection release piece 53 includes supporting plate 533 and taking off with supporting plate 533 and ejecting From the connecting rod 534 that plate 531 connects, supports plate 533 and be located between the driving portion and main body 512 of sampling gun actuator 52, support Plate 533 and ejection disengaging plate 531, which pass through connecting rod 534, fixed pedestal 521 to be arranged in up and down along the height direction H On, driving portion can abut under the retracted mode with plate 533 is supported, to push ejection release piece 53 along the disengaging side To movement, connecting rod 534 is resiliently supported on fixed pedestal 521 by the reset spring 535 being set in the connecting rod 534, The both ends of reset spring 535 respectively with support plate 533 and fixed pedestal 521 abuts, to drive ejection to be detached from always for providing Part 53 returns back to the elastic-restoring force of initial position.Wherein, connecting rod mounting hole 5211 can be set on fixed pedestal 521, Connecting rod 534 is cooperated in connecting rod mounting hole 5211 by structures such as axle sleeves 5212, in 534 lifting process of connecting rod The structures such as axle sleeve 5212 play the role of protecting connecting rod 534 and effectivelying prevent connecting rod 534 that movement abrasion occurs.Wherein, it is Convenient for all parts of assembly pipette tips ejection releasing mechanism 5, and make the structure cloth for ejecting release piece 53 and sampling gun 51 It sets and more rationalizes, optionally, support and could be formed with the telescopic rod holding tank passed through for each telescopic rod 511 on plate 533 5331, here, under the original state that ejection release piece 53 is in idle, the elastic anchorage force that is provided by reset spring 535 So that ejection release piece 53 is supported integrally on fixed pedestal 521, in the case where ejection release piece 53 is in running order, sampling gun is driven It the driving portion of moving part 52 and supports plate 533 and is in contact and the guiding role by connecting rod 534 on fixed pedestal 521 So that ejection release piece 53 is whole mobile towards off-direction (i.e. in height direction H downwards), in the process, ejection is detached from Plate 531 and each sampling hammerhead 200 are in contact and each sampling hammerhead 200 are pushed to make it from corresponding sampling gun 51 Pipette tips interconnecting piece 514 falls off.In addition, being removed in sampling gun actuator 52 to supporting after the unloading for completing sampling hammerhead 200 When the active force of top plate 533, ejection release piece 53 is returned to idle entirely through the elastic-restoring force of reset spring 535 Initial position.As a result, by structure as described above can handling operation that is accurate and fast implementing sampling hammerhead 200, effectively Guarantee that nucleic acid extraction operation operates normally.But it's not limited to that for the disclosure, and the specific structure for ejecting release piece 53 can be with According to the actual arrangement structure of sample processing device come reasonable design, for example, for the driving method for ejecting release piece 53, it can It is not generated so that other driving source is used alone with sampling gun actuator 52 and linkedly directly drives the ejection movement of release piece 53 And to eject release piece 53 and the cooperation of sampling hammerhead 200 and realize the unloading of sampling hammerhead 200 from pipette tips interconnecting piece 514.
Here, in order to further increase nucleic acid extraction equal samples treatment effeciency, optionally, as shown in figure 12, sampling gun 51 X2 has been positioned apart from multiple in transverse direction, supports the X2 extension in transverse direction respectively of plate 533 and ejection disengaging plate 531, even Extension bar 534 is that mode that is multiple and being separated with a sampling gun 51 between X2 in transverse direction respectively is arranged in and supports plate 533 and ejection Between disengaging plate 531, divide between plate 533, ejection disengaging plate 531 and every adjacent every two connecting rod 534 so that supporting Not Gou Cheng sampling gun layout area, the main body 512 of each sampling gun 51 is located in the sampling gun layout area.Wherein, each It can be arranged with reset spring 535 as described above in connecting rod 534, enhance and the whole resilient support of ejection release piece 53 is imitated Fruit to support plate 533 and ejection disengaging plate 531 is firmly connected as overall structure, improves ejection by multiple connecting rods 534 The whole bonding strength of release piece 53 and rigidity, effectively avoid occuring bending and deformation during pushing multiple sampling hammerheads Phenomenon.In addition, in the specific embodiment of the disclosure, for example, sampling gun 51 can be positioned apart from four with X2 in transverse direction It is a, and there are four telescopic rod channel 513 and four telescopic rods 511 for setting in each sampling gun 51, it can be by as described above Four sampling guns 51 and the nucleic acid extraction equal samples processing operation that disposably can synchronously execute 16 samples, and pass through The disposable synchronously execution unloading of 16 sampling hammerheads 200 is enabled to make using plate 533 and ejection disengaging plate 531 is supported Industry, so that ejection release piece 53 is more simple for the unloading operation of sampling hammerhead 200 and is easily achieved.
Here, optionally, as shown in figure 18, each pipette tips interconnecting piece 514 includes the bottom end opening with telescopic rod channel 513 The seal section 516 for sealing cooperation, the pipette tips linkage section 515 to connect with the seal section 516, being interval in for pipette tips linkage section 515 are close The locking protrusion 517 being formed on the position of section 516 for being engaged by clamping with sampling hammerhead 200 is sealed, ejection disengaging plate 531 is located at Between seal section 516 and locking protrusion 517, locking protrusion 517 is arranged to be inserted into hole 532 across pipette tips interconnecting piece.? This, it is for the ease of processing and assemble pipette tips that the main body 512 of pipette tips interconnecting piece 514 and sampling gun 51, which is arranged to separate structure, The all parts of releasing mechanism 5 are ejected, and also has the effect of convenient for follow-up maintenance and reduces maintenance cost.In addition, will When sampling hammerhead 200 is loaded into pipette tips linkage section 515, due to the locking protrusion 517 and sampling hammerhead on pipette tips linkage section 515 200 are engaged by clamping, to guarantee that sampling hammerhead 200 holds normal imbibition and draining function, at nucleic acid extraction equal samples Avoid there is a phenomenon where sampling hammerheads 200 to be detached from during reason from pipette tips linkage section 515.
Optionally, as shown in figure 19, sampling gun actuator 52 includes fixed pedestal 521, is arranged on the fixed pedestal 521 Sampling gun driving motor 522, with the transmission of the output shaft of sampling gun driving motor 522 be connected and be provided with the sampling gun of driving portion Transmission mechanism converts the rotary motion of sampling gun driving motor 522 to the linear motion of driving portion.Wherein, fixed pedestal 521 can be set on the pedestal 411 of pipette tips mobile device 41, and sampling gun transmission mechanism can use the arrangement knot of various reasonable Structure, as long as can be realized the function of converting the rotary motion of sampling gun driving motor 522 to the linear motion of driving portion, For example, sampling gun transmission mechanism can be rack pinion matching mechanism, Worm Wheel System matching mechanism, worm drive machine Structure etc..Here, optionally, sampling gun transmission mechanism is screw-and-nut mechanism, including defeated with sampling gun driving motor 522 It axis connection and the first screw rod 523 being pivotally supported on fixed pedestal 521 and can movably be set along height direction H out The first nut 524 on the first screw rod 523 is set, driving portion is the driving baffle being set on the outer peripheral surface of the first nut 524 525, the holding tank 526 on the head for clamping telescopic rod 511 is formed on the driving baffle 525.Here, the first screw rod 523 It can be pivotally supported at by bearing on fixed pedestal 521, here, for the ease of the first nut of connection 524 and driving gear Plate 525, the first nut 524 can be formed as T-nut, and driving baffle 525 is set on the small head end of the T-nut and passes through Bolt and the stub end of T-nut are fixed together, and the connection of driving baffle 525 and the first nut 524 has thus been effectively ensured Reliability.In addition, X2 extends and can be formed driving baffle 525 in transverse direction in the case where sampling gun is provided with multiple For with the development length roughly the same with the development length for supporting plate 533, to enable to driving baffle 525 to supporting plate 533 apply uniform power, are effectively ensured to support plate 533, connecting rod 534 and eject 531 entirety of disengaging plate and put down along height direction H Quietly move.In addition, for the ease of the telescopic rod 511 of sampling gun 51 to be assembled on driving baffle 525, optionally, holding tank 526 are formed to have the C-channel of opening, and the head of telescopic rod 511 circumferentially could be formed with the inside for snapping fit onto C-channel Clamping engagement groove on wall, thus telescopic rod 511 is by snapping fit onto C-channel clamping engagement slot from the opening of C-channel, by While this guarantees telescopic rod 511 and drives the assembly reliability between baffle 525, realize to telescopic rod 511 relative to drive Dynamic limit of the baffle 525 in height direction H.Have by using screw-and-nut mechanism as described above and matches occlusal wear It is small, transmission efficiency is high, stable drive, service life are long and advantage with high accuracy.
In order to meet the lightweight of pipette tips ejection releasing mechanism 5 and save the demand of manufacturing cost, optionally, fixed base Seat 521 is including vertical plate 527 and along height direction H arranged for interval in the upper water plate 528 of the side of vertical plate 527 and lower part Level board 529, sampling gun driving motor 522 and sampling gun transmission mechanism are arranged on upper water plate 528, eject release piece 53 It can be arranged in up and down on lower horizontal plate 529 along height direction H, driving baffle 525 is located at upper water plate 528 and lower part Between level board 529.Wherein, main body holding tank 5291, the main body 512 of sampling gun 51 be could be formed on lower horizontal plate 528 It can be received into the main body holding tank 5291 and be fixed on lower horizontal plate 528 by fasteners such as bolts, be therefore saved on The arrangement space of pipette tips ejection releasing mechanism 5.In addition, being taken using bolt and nut drive mechanism as described above Sample rifle driving motor 522 is mounted on the top of upper water plate 528, the output of the first screw rod 523 and sampling gun driving motor 522 Axis connection simultaneously is arranged as successively penetrating through upper water plate 528 and driving baffle 525 along height direction H, here, in order to which stabilization is led To driving the moving in height direction H of baffle 525, optionally, sampling gun driving motor is located in upper water plate 528 The guide rod 5281 that perforation driving baffle 525 can be set on the position of 522 two sides, drives baffle 525 and guide rod Guideway 5282 is provided between 5281.In addition, being also provided on upper water plate 528 for detecting driving baffle The baffle position sensor 5283 of 525 position, for example, as shown in figure, baffle position sensor 5283 can be photoelectric transfer Sensor, specifically can be using groove profile photoelectrical position sensor etc., here, baffle position sensor 5283 for example can detecte High limit position of the baffle 525 in height direction H is driven, whether to detect the telescopic rod 511 of sampling gun 51 in complete Stretching state.Correspondingly, it can also be provided on lower horizontal plate 529 for detecting driving baffle 525 in height direction H Most sole extreme position position sensor, to detect whether the telescopic rod of sampling gun 51 is in fully retracted state.
When pipette tips as described above ejection releasing mechanism is adapted to pipette tips mobile device 41 as described above, such as Figure 12 It, can be by enabling pipette tips to eject releasing mechanism 5 along height side fixed pedestal 521 as described above to shown in Figure 15 It is arranged on pedestal 411 up and down to H, here, for example, pedestal 411 can be formed as gantry shape, at the top of pedestal 411 Releasing mechanism driving motor 412 is set, which passes through releasing mechanism screw-and-nut mechanism 413 It is connected with the transmission of fixed pedestal 521, that is, the screw rod of releasing mechanism screw-and-nut mechanism 413 extends and passes through along height direction H The assembling stand 5211 of logical fixed pedestal 521, here, stablizing guiding pipette tips ejection 5 (specially assembling stand of releasing mechanism to play 5211) moving along height direction H as illustrated in fig. 15 can be in pedestal 411 positioned at releasing mechanism driving motor 412 The releasing mechanism guide frame 414 of movement for being oriented to pipette tips ejection releasing mechanism 5 is set on two side positions.The releasing mechanism Guide frame 414 is identical as the structure of guide rod 5281 as described above and guideway 5282, and details are not described herein.In addition, The fixed pedestal position sensor 415 of the position for detecting fixed pedestal 521 can be provided on pedestal 1, thus, it is possible to High limit position of the detection pipette tips ejection releasing mechanism 5 in height direction H out.
To sum up, feature, mode of texturing and the function and effect etc. of pipette tips ejection releasing mechanism are described in detail, and above-mentioned retouch It states and is mainly illustrated with embodiment of the pipette tips ejection releasing mechanism suitable for above-mentioned pipette tips mobile device 41, but this Open it's not limited to that, and the pipette tips ejection releasing mechanism of structure as described above can be applicable to be used for the first work as described above Make in the first mechanical arm 6 of region A and the second mechanical arm 7 for third working region C, it is in the case, mobile with pipette tips Correspondingly, first mechanical arm 6 and second mechanical arm 7 can pass through well known motor and screw-and-nut mechanism to device 41 respectively Mutually matched multiple groups transmission module, realize along being parallel to the longitudinal direction X1 of microscope carrier moving direction Z1 and perpendicular to the load The adjustment position of the transverse direction X2 of platform moving direction Z1, and the lifting along height direction H.In this regard, omitting to the detailed of its Explanation.
Optionally, the magnetic bead transfer device 42 as shown in Figure 12, Figure 20 and Figure 21, in nucleic acid extraction mechanism 4 as described above Including magnetic bead transfer device fixing seat 421 of the microscope carrier transport establishment 1 on the position of sampling hammerhead 200 is arranged in, setting exists Magnetic part actuator and magnetic part movable plate 422 in magnetic bead transfer device fixing seat 421, the magnetic part movable plate 422 are set It sets in magnetic bead transfer device fixing seat 421, and connect with magnetic part actuator with can be along close to or far from sampling hammerhead 200 direction is flexible, and magnetic part is magnet 423 and is mounted on magnetic part movable plate 422 close to the side of sampling gun 51.Here, In the case that nucleic acid extraction mechanism 4 as described above is suitable for sample processing device, the magnetic bead of magnetic bead transfer device 42 is shifted Device fixing seat 421 can be set on the second working region B of microscope carrier transport establishment 1, such as can be set and move in microscope carrier It is located at the front side of pipette tips mobile device 41 on dynamic direction Z1, is arranged on the pipette tips ejection releasing mechanism 5 of pipette tips mobile device 41 In the case where having multiple sampling guns 51, magnet 423 can also be positioned apart from more on the position of magnetic part movable plate 422 It is a, wherein the sampling hammerhead 200 loaded on each magnet 423 and each sampling gun 51 is opposite, can effectively ensure that each take Sample pipette tips 200 can receive the effect of magnetic field force, and then guarantee the extraction quality of nucleic acid.Wherein, magnetic part actuator uses For the structure of various reasonable, for example, magnetic part actuator can be telescoping cylinder, to drive magnetic part movable plate 422 towards close Or the direction far from sampling hammerhead 200 is flexible, or, optionally, magnetic part actuator includes 426 He of magnetic part driving motor Magnetic part bolt and nut mechanism, magnetic part driving motor 426 are arranged in magnetic bead transfer device fixing seat 421, magnetic part screw rod The magnetic part screw rod 427 of nut body and the output axis connection of the magnetic part driving motor 426, magnetic part bolt and nut mechanism Magnetic part nut 428 is connect with magnetic part movable plate 422.The simple magnetic part actuator of structure as described above, which has, as a result, passes The features such as dynamic steady, use reliability is high.
In addition, pipette tips mobile device 41 as described above and magnetic bead transfer device 42 are suitable for sample as described above In the case where processing equipment, such as in the second working region B, the step-by-step movement by microscope carrier 2 along nucleic acid extraction direction Z2 is moved Come when realizing nucleic acid extraction operation, magnetic bead transfer device 42 can be on the Z2 of nucleic acid extraction direction positioned at magnetic bead transfer device 42 Downstream side, that is, specifically, for example, nucleic acid extraction direction Z2 is in contrast to the microscope carrier referred in sample processing device as described above In the case where moving direction Z1, magnetic bead transfer device 42 is located at the front side of pipette tips mobile device 41 on microscope carrier moving direction Z1, To avoid the moving along nucleic acid extraction direction Z2 in the second working region B of microscope carrier 2, at this point, since microscope carrier 2 is in nucleic acid extraction mistake Be directed away from journey magnetic bead transfer device 42 direction movement, therefore, magnetic bead transfer device 42 can according to sampling hammerhead 200 Between actual range (such as can be according to moving distance of microscope carrier 2) come adjust in real time towards sampling hammerhead 200 stretch out Degree is effectively ensured and provides magnetic force to sampling hammerhead 200, or carries out nucleic acid extraction mistake in the second working region B in microscope carrier 2 The overall distance that Z2 is moved along nucleic acid extraction direction in journey is smaller, and makes the magnetic force not shadow substantially applied to sampling hammerhead 200 Under the premise of ringing absorption magnetic bead, the magnetic part of magnetic bead transfer device 42 can be made repeatedly to stretch in the second working region B Degree keeps identical.
Here, being moved in order to being steadily oriented to magnetic part movable plate 422, and convenient for assembly magnetic part movable plate 422 and magnetic bead device device fixing seat 421 optionally as shown in figure 21, be formed on magnetic part movable plate 422 along magnetic part The long guiding hole 424 that the telescopic direction of movable plate 422 extends is provided on magnetic bead transfer device 42 and leads for being inserted into and being installed to Positioning screw 425 into long hole 424, magnetic part movable plate 422 can by the cooperation of long guiding hole 424 and positioning screw 425 Telescopically it is arranged on magnetic bead transfer device 42.Here, mobile panel guide can be set in magnetic bead transfer device fixing seat 421 4211, magnetic part movable plate 422 can play dual guiding role by the cooperation of sliding block and mobile panel guide 4211.? This can be set in magnetic bead transfer device fixing seat 421 to accurately control the stretching displacement of magnetic part movable plate 422 For detecting the magnetic part movable plate position sensor such as photoelectrical position sensor of the extended position of magnetic part movable plate 422 429。
To sum up, nucleic acid extraction mechanism 4 is turned by the lifting action and magnetic bead of the pipette tips mobile device 41 of structure as described above The cooperation of the expanding-contracting action of moving device 42, the mode that bead complexes as described above follow sampling hammerhead 200 mobile always are held Row nucleic acid extraction operation, and since the nucleic acid extraction of each sample only needs to can be realized as by a sampling hammerhead, thus Sampling hammerhead consumptive material is saved, nucleic acid extraction cost is reduced.In addition, due to the nucleic acid extraction mechanism 4 to be fixed on microscope carrier fortune Under stationary state in second working region B of transfer mechanism 1, cell cracking is successively executed by mobile sample container 3, is washed The nucleic acid extractions operation such as wash, elute, thus convenient for operation nucleic acid extraction mechanism 4, so that the entire control of sample processing device is grasped Tend to simplify as mode, be more easily implemented automation control.
In addition, being based on nucleic acid extraction mechanism 4 as described above, the disclosure also provides a kind of utilization nucleic acid as described above and mentions The method for extracting nucleic acid of mechanism 4 is taken, the method for extracting nucleic acid includes: cleavage step S21, and sampling hammerhead 200 draws sample simultaneously The sample is added in the cell pyrolysis liquid containing magnetic bead into sample container 3, so that obtaining adsorption after sample cracking has The bead complexes of nucleic acid, sampling hammerhead draws the solution containing bead complexes, in magnetic part close to the side of sampling hammerhead 200 To stretching under magnetic state, sampling hammerhead 200 spues in addition to the bead complexes being adsorbed on 200 inner wall of sampling hammerhead Solution；Washing step S22, under the demagnetizing state that magnetic part is retracted far from the direction of sampling hammerhead 200, sampling hammerhead 200 is inhaled It samples the cleaning solution in this container 3 and executes compressing movement repeatedly, to clean bead complexes, later under for magnetic state, Sampling hammerhead 200, which spues, removes the solution containing bead complexes；Elution step S23, under demagnetizing state, sampling hammerhead 200 is inhaled It samples the eluent in this container 3 and executes compressing movement repeatedly, so that magnetic bead and nucleic acid separation in bead complexes, Sampling hammerhead 200 draws the solution containing magnetic bead and nucleic acid；Nucleic acid extraction step S24, under for magnetic state, sampling hammerhead 200 By except the nucleic acid containing magnetic bead spues and saves to sample container 3, method for extracting nucleic acid is in sample container 3 with step-by-step movement In traveling process, by the cooperation of the expanding-contracting action of the lifting action and magnetic bead transfer device 42 of pipette tips mobile device 41, successively It is inserted into the holding tank of each sample container 3 and executes cleavage step S21, washing step S22, elution step S23 and core Sour extraction step S24 and obtain nucleic acid.
Here, the process of nucleic acid extraction for ease of description, by each sample holding tank of sample container 3 be defined as Under, that is, the sample holding tank arranged along microscope carrier moving direction Z1 is defined respectively and is used to accommodate the cell of cell pyrolysis liquid and splits It solves liquid holding tank, the cleaning solution holding tank for accommodating cleaning solution, the eluent holding tank for accommodating eluent and is used for Accommodate the nucleic acid holding tank of the nucleic acid extracted.As a result, when sample container 3 is moved along nucleic acid extraction direction Z2, pipette tips are mobile The sampling hammerhead 200 loaded on device 41, which can be sequentially inserted into cell pyrolysis liquid holding tank, cleaning solution holding tank, eluent, to be held Receive slot and nucleic acid holding tank.
As described above, the method for extracting nucleic acid of the disclosure extracts nucleic acid using paramagnetic particle method, can be realized high-throughput operation and Automation, and the high-affinity for having used inorganic particle to be formed in conjunction with high molecular material due to paramagnetic particle method nucleic acid extraction Composite magnetic microballoon (magnetic bead), the composite magnetic microballoon have both numerous characteristics of polymer microsphere and magnetic particle, therefore are not having Having can be uniformly dispersed in the solution in the case where applying magnetic field, stablize, can be simple and quick in the case where applying magnetic field Ground is separated with solution, is specifically bound with what is calculated so that the nucleic acid purity extracted is high, dense from there through composite magnetic microballoon Degree is big, and with high security, it is low in cost and the advantages of convenient for being widely applied.
In the method for extracting nucleic acid of the disclosure, in sample container 3 to be filled by the way that pipette tips are mobile in step-by-step movement traveling process The cooperation for setting 41 lifting action and the expanding-contracting action of magnetic bead transfer device 42 is sequentially inserted into the appearance of each sample container 3 It receives in slot and executes the nucleic acid extraction operation of interdependent ground.Specifically, the state of sampling hammerhead 200 is loaded in pipette tips mobile device 41 Under, sampling hammerhead 200 declines after drawing sample along height direction H and be inserted into sample container 3 accommodates cell pyrolysis liquid In the cell pyrolysis liquid holding tank of magnetic bead, sample is expelled in cell pyrolysis liquid holding tank and executes and split by sampling hammerhead 200 Solve step S21.In cleavage step S21, sampling hammerhead 200 executes compressing movement so that sample and cell cracking repeatedly Liquid is sufficiently mixed, and quiet to put a period of time so that sufficiently dissolution occurs for the cell of sample, later, sample is released after carrying out cell cracking The nucleic acid of releasing is mixed with magnetic bead, forms the solution for containing nucleic acid-bead complexes (hereinafter referred to as bead complexes).It takes Sample pipette tips 200 draw the cracking waste liquid containing bead complexes, and in the case where magnetic part is for magnetic state, nucleic acid-magnetic bead is compound Object can be adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device 41 drives the discharge of sampling hammerhead 200 except magnetic bead is multiple Close the waste reaction solution except object.After completing cleavage step S21, pipette tips mobile device 41 drive sampling hammerhead 200 rise and It is detached from cell pyrolysis liquid holding tank, sample container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement later, so that sample accommodates The cleaning solution holding tank for accommodating cleaning solution of device 3 is located at the underface of sampling hammerhead 200, the driving sampling of pipette tips mobile device 41 Pipette tips 200 decline and are inserted into cleaning solution holding tank, execute washing step S22.In washing step S22, in magnetic part Demagnetizing state under, sampling hammerhead 200 execute repeatedly compressing movement so that complete cleavage step S21 bead complexes with wash It washs liquid to be sufficiently mixed, achievees the effect that thoroughly to clean impurity.Later, sampling hammerhead 200 draws the washing containing bead complexes Waste liquid, in the case where magnetic part is for magnetic state, bead complexes are adsorbed on the inner wall of sampling hammerhead 200, at this point, the mobile dress of pipette tips The discharge of 41 driving sampling hammerheads 200 is set in the scrub raffinate to cleaning solution holding tank in addition to bead complexes.Here, for magnetic The washing step S22 of pearl compound can be executed repeatedly, such as twice repeatedly, in the case, in sample container 3 can also be with It is correspondingly provided with multiple such as two cleaning solution holding tanks.After completing to the washing step S22 of bead complexes, pipette tips Mobile device 41 drives sampling hammerhead 200 to rise and be detached from cleaning solution holding tank, and sample container 3 is along nucleic acid extraction direction later Z2 is mobile with step-by-step movement, so that the eluent holding tank for accommodating eluent of sample container 3 is being located at sampling hammerhead 200 just Lower section, pipette tips mobile device 41 drive sampling hammerhead 200 to decline and are inserted into eluent holding tank, execute elution step S23. In elution step S23, under the demagnetizing state of magnetic part, sampling hammerhead 200 executes compressing movement so that completing repeatedly The bead complexes of washing step S22 are sufficiently mixed with eluent, achieve the effect that the extracting nucleic acid from magnetic bead.Sampling hammerhead 200 draw the solution containing magnetic bead and nucleic acid.After completing to execute elution step S23, the driving sampling of pipette tips mobile device 41 Pipette tips 200 rise and are detached from eluent holding tank, and sample container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement later, is made The nucleic acid holding tank for obtaining sample container 3 is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives sampling hammerhead 200 decline and are inserted into nucleic acid holding tank, execute nucleic acid extraction step S24.In nucleic acid extraction step S24, in magnetism Part under magnetic state, the discharge of sampling hammerhead 200 is in the nucleic acid solution to nucleic acid holding tank in addition to magnetic bead.Thus it completes entire Nucleic acid extraction operation.
To sum up, through method for extracting nucleic acid as described above during nucleic acid extraction, nucleic acid-magnetic bead as described above is multiple It closes object and follows the movement of sampling hammerhead 200 always, therefore the nucleic acid extraction of each sample is only needed through a sampling hammerhead energy It is enough to realize, sampling hammerhead consumptive material is thereby saved, nucleic acid extraction cost is reduced.In addition, during nucleic acid extraction, due to rifle 41 drive sampling hammerheads 200 of head moving device can make magnetic bead transfer device 42 along height direction H shift position In the state of relatively fixed in microscope carrier transport establishment 1, it is convenient for by the expanding-contracting action of the magnetic part of magnetic bead transfer device 42 Magnetic force or removal magnetic force are provided to bead complexes, so that nucleic acid extraction simple operation, reduces nucleic acid extraction mechanism And the manufacture and processing cost of sample processing device.
Optionally, in cleavage step S21, after sampling hammerhead 200 draws the solution containing bead complexes, further include Protein digestibility step S25, under demagnetizing state, sampling hammerhead 200 spues the solution containing bead complexes to the sample It in protein digestibility enzyme solutions in this container 3 and executes compressing repeatedly to act, with the protein in digestion solution, Zhi Hou The solution containing bead complexes is removed under magnetic state, the sampling hammerhead 200 spues, to complete the cleavage step S21。
Here, can be set between cell pyrolysis liquid holding tank and cleaning solution holding tank for holding in sample container It receives the protein digestibility enzyme solutions holding tank containing protein kinase, accommodates splitting containing bead complexes in sampling hammerhead 200 In the state of liquid waste solution, pipette tips mobile device 41 drives sampling hammerhead 200 to rise and be detached from cell pyrolysis liquid holding tank, later sample This container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement, so that sample container 3 accommodates the egg containing protein kinase White matter digestion enzyme solutions holding tank is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives sampling hammerhead 200 to decline And be inserted into protein digestibility enzyme solutions holding tank, execute protein digestibility step S25.In protein digestibility step S25 In, under the demagnetizing state of magnetic part, sampling hammerhead 200 executes compressing movement so that containing the magnetic for completing cell cracking repeatedly The cracking waste liquid of pearl compound is sufficiently mixed with the protein digestibility enzyme solutions containing protein kinase, reaches the egg in digestion solution The effect of white matter.Later, sampling hammerhead 200 draws the waste reaction solution containing bead complexes, in the case where magnetic part is for magnetic state, Bead complexes are adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device 41 drives the discharge of sampling hammerhead 200 to remove In waste reaction solution to protein digestibility enzyme solutions holding tank except bead complexes, to complete cleavage step S21 and execute Next step, that is, washing step S22.By step as described above, nucleic acid extraction quality can be further increased, guarantees sample The accuracy of processing.
According to another aspect of the present disclosure, following microscope carrier and microscope carrier fortune can be applicable in the microscope carrier processing equipment of the disclosure The cooperation assembly of transfer mechanism.Referring to as shown in Figure 22 to Figure 29, wherein the cooperation assembly of the disclosure, which uses, passes through other structures The microscope carrier passive type driving method for driving microscope carrier 2 to move, that is, microscope carrier and the cooperation assembly of microscope carrier transport establishment include 2 He of microscope carrier Microscope carrier transport establishment 1, microscope carrier driving mechanism is provided in microscope carrier transport establishment 1, and microscope carrier 2 is movable by microscope carrier driving mechanism Ground is arranged in microscope carrier transport establishment 1, can adjust the working region locating in microscope carrier transport establishment 1 of microscope carrier 2.Here, It is passive using driving microscope carrier 2 to move by microscope carrier driving mechanism in microscope carrier processing equipment and cooperation assembly as described above Formula microscope carrier driving method here, microscope carrier driving mechanism can use any suitable arragement construction, such as can be independent one A mechanism also may include multiple independent mechanisms, each mechanism can independent realization driving microscope carrier 2 transported in microscope carrier The function of moving in mechanism 1 for another example may be used for example, the function of movement of the driving microscope carrier 2 in microscope carrier transport establishment 1 may be implemented To realize the function of driving microscope carrier 2 to go up and down in microscope carrier transport establishment 1, as long as microscope carrier driving mechanism can be realized driving microscope carrier 2 Move and adjust the function of the working region locating in microscope carrier transport establishment 1 of microscope carrier 2.By structure as described above, I.e. in sample process, microscope carrier 2 can be made to move in microscope carrier transport establishment 1 by microscope carrier driving mechanism, thus executed During sample process so that microscope carrier 2 be sequentially located in corresponding each working region of microscope carrier transport establishment 1 and with it is corresponding Mechanical arm cooperation, thereby executing corresponding sample process work, thus the above-mentioned cooperation assembly of the disclosure with it is existing in pass through The movement of mechanical arm is compared come the mode successively to fixed microscope carrier realization sample process, enables to the movement of corresponding mechanical arm Path and operation are more simple, and the disclosure passes through movement of the microscope carrier 2 relative to microscope carrier transport establishment 1, in multiple microscope carriers 2 successively execute sample process in the case where so that multiple microscope carriers 2 successively moved in microscope carrier transport establishment 1 and in each work Only by a mechanical arm multiple microscope carriers 2 can successively be executed with corresponding work in region, thus save whole manufacture and Sample process efficiency is also improved while processing cost.
Optionally, as shown in figure 22, microscope carrier driving mechanism includes for driving microscope carrier 2 to move along microscope carrier moving direction Z1 Microscope carrier driven in translation structure 12, microscope carrier driven in translation structure 12 include the first driving motor 121 and first driving motor 121 The first connected transmission component of output shaft transmission and connect and can separatably cooperate with microscope carrier 2 with the first transmission component Movable trolley 122, the first transmission component is used to the rotary motion of the first driving motor 121 being converted to movable trolley 122 Linear movement, to enable to movable trolley 122 to adjust the institute in microscope carrier transport establishment 1 of microscope carrier 2 by the cooperation with microscope carrier 2 The working region at place.Here, the first transmission component can use the structure of various reasonable, for example, optionally, the first transmission component It can be V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission matching mechanism, thus, it is possible to improve transmission Reliability and transmission efficiency.In structure as described above, movable trolley 122 and the departing fit system of microscope carrier 2 can be with Various structures are used, for example, can be by the way that telescopic stretching structure is arranged on movable trolley 122, to pass through stretching structure It stretches out and is inserted into the mode on microscope carrier 2 to realize the relatively fixed of movable trolley 122 and microscope carrier 2, retracted by stretching structure And the mode of microscope carrier 2 is detached to realize the relative motion of movable trolley 122 and microscope carrier 2；It for another example, can be by movable trolley The clamping element that can clamp is set on 122, movable trolley 122 and microscope carrier 2 are realized in a manner of clamping microscope carrier 2 by clamping element It is relatively fixed, the relative motion of movable trolley 122 and microscope carrier 2 is realized in such a way that clamping element discharges microscope carrier 2.But disclosure is simultaneously It is not limited to this, movable trolley 122 can carry out reasonable design with the departing fit system of microscope carrier 2 according to actual needs.It is logical Structure as described above is crossed, that is, starts the first driving motor 121 and outputs it the rotary force of power axis and pass through the first transmission component Transmit and be converted to the linear movement of movable trolley 122, reliable mobile of the guarantee activity trolley 122 in microscope carrier transport establishment 1, And can steadily drive microscope carrier 2 to move to each working region, it ensure that the transportation of microscope carrier 2.
Optionally, the first transmission component is V belt translation matching mechanism, and is passed including the first transmission belt 123, setting first Move 123 side of band and with the first driving wheel 124 of the output axis connection of the first driving motor 121 and setting in the first transmission belt First driven wheel 125 of 123 other sides, movable trolley 122 are mounted on the first transmission belt 123.Wherein, V belt translation matching mechanism Have many advantages, such as that stable drive, buffering absorbing, structure is simple, at low cost, working service is convenient, in microscope carrier transport establishment 1, carries In the longer situation of the moving range of platform 2, only allowed for by the V belt translation matching mechanism of simple structure as described above First transmission belt 123 is reliable and easily drive activity trolley 122 realizes flexible movement in moving range.
In order to remain movable trolley 122 during 123 drive activity trolley 122 of the first transmission belt is mobile Do linear movement, and avoid because the first transmission belt 123 is too long lead to deformation due to there is microscope carrier 2 movement routine change, can Selection of land, as shown in Figure 24 to Figure 29, the downside in microscope carrier transport establishment 1 positioned at the first transmission belt 123 is provided with moves along microscope carrier The trolley guide rail 126 that direction Z1 extends, movable trolley 122 are fixed in the downside band part of the first transmission belt 123, movable trolley 122 bottom is provided with the second pulley component 127 slideably cooperated with trolley guide rail 122.Movable trolley 122 passes through second Pulley assembly 127 rollably supports on trolley guide rail 126, so that trolley guide rail 126 can be smoothly directing to movable trolley 122 linear movement on microscope carrier moving direction Z1, simultaneously, additionally it is possible to cooperate more flexible by rolling and easily realize Movable the moving along microscope carrier moving direction Z1 of trolley 122.
Here, optionally, such as scheming for the installation reliability being effectively ensured between movable trolley 122 and trolley guide rail 126 Shown in 30, the bottom of movable trolley 122, second pulley peace are arranged in by second pulley mounting base 128 for second pulley component 127 Dress seat 128 is located at 123 downside of the first transmission belt and is supported on trolley guide rail 126 by second pulley component 127.But the disclosure It's not limited to that, and alternatively, the bottom of movable trolley 122 can be formed directly with for for the first transmission belt The transmission belt mounting groove that 123 lower portion passes through, second pulley component 127 can be mounted directly or be formed in movable trolley 122 bottom.
Optionally, stretching structure 8 is telescopically provided on movable trolley 122, microscope carrier 2 corresponds to movable trolley 122 The locking structure 21 being mutually locked for cooperating with stretching structure 8 is provided on the side wall of side.Here, for stretching structure 8 The arrangement of various reasonable can be used with the structure of locking structure 21, as long as can by the flexible of stretching structure 8 Cooperate or separate with locking structure 21, so that microscope carrier 2 is locked on movable trolley 122 or is detached from from movable trolley 122. During sample process in the case where a certain working region for needing for microscope carrier 2 to be moved to microscope carrier transport establishment 1, activity Trolley 122 drives microscope carrier 2 to be moved to a certain working region by the cooperation of stretching structure 8 and locking structure 21, wherein In the case that microscope carrier 2 is multiple, movable trolley 122 can pass through the cooperation of stretching structure 8 and locking structure 21 and disengaging Mode successively drives multiple microscope carriers 2 to be moved to corresponding working region, so that microscope carrier 2 is consecutively carried out sample process Operation improves the operating efficiency of whole sample process.Here, for example, stretching structure 8 can using electric cylinder, hydraulic cylinder, The flexible cylinder structure such as pneumatic linear actuator, for another example, stretching structure 8 can also be using screw pairs, rack-and-pinion, snails such as motor and feed screw nuts The matching mechanism of the drive mechanisms such as worm and gear is matched in such a way that the driving by motor is so that each bar is flexible with locking structure 21 It closes.
Here, for another example, optionally, as shown in Figure 30 to Figure 32, stretching structure 8 includes the second magnet plunger 81, the second electromagnetism Bar 81 has lockup state and unlocked state, in lockup state, the second magnet plunger 81 power-off, so that the second magnet plunger 81 pushes away Bar is stretched out and is snapped on locking structure 21 and locking microscope carrier 2 and movable trolley 122；In unlocked state, the second magnet plunger 81 is logical Electricity, to make pusher retracted unlock microscope carrier 2 and movable trolley 122, locking knot by the magnetic force generated in the second magnet plunger 81 Structure 21 is formed to have lock groove or locking hole with the shape of the second magnet plunger 81 cooperation.Thus, it is possible to make movable table vehicle Reliable locking is realized between 122 and microscope carrier 2, and movable trolley 122 is able to drive microscope carrier 2 and is moved to exact position, passes through Structure as described above can effectively improve the operational reliability of movable trolley 122.In addition, in order to further increase movable table The locking reliability of vehicle 122 and microscope carrier 2, as shown in Figure 30 to Figure 32, stretching structure 8 can also include electromagnet 82, the electricity Magnet 82 be two and be separately positioned on the movable trolley 122 positioned at the position of 81 two sides of the second magnet plunger on, load Platform 2, which corresponds on the side wall of the electromagnet 82, is formed with electromagnet latch 28 for cooperating with the electromagnet 82, The lockup state, the electromagnet 82 is powered and the magnetic force by generating is adsorbed onto the electromagnet latch 28, with Microscope carrier 2 described in locking and the movable trolley 122；In the unlocked state, the electromagnet 82 powers off and is detached from the electromagnetism Iron latch 28, to unlock the microscope carrier 2 and the movable trolley 122.Here, electromagnet 82 can use magnechuck etc., Pass through structure as described above, that is, the second magnet plunger 81 and electromagnet 82 it is synchronous stretch out or it is synchronous retract by way of with Microscope carrier 2 realizes secure ratcs or unlock.
Optionally, described to match as shown in Figure 22 to Figure 27, Figure 32 and Figure 33 according to the first embodiment of the disclosure Closing assembly includes the microscope carrier location structure, and the microscope carrier location structure includes 22 He of the first positioning seat being arranged on microscope carrier 2 The second positioning seat 15 in microscope carrier transport establishment 1 is set, is formed with the first positioning region, the second positioning seat on the first positioning seat 22 Multiple second positioning regions for cooperating or separating with the first positioning region are positioned apart from along microscope carrier moving direction Z1 on 15, It, can be fixed with corresponding second when first positioning region is located at the position opposite with any one the second positioning region in the second positioning region Position portion cooperates and positions.As described above, microscope carrier 2 in microscope carrier transport establishment 1 along microscope carrier moving direction Z1 moving process, first When positioning region is placed exactly in an opposite position in the second positioning region in the second positioning regions multiple in microscope carrier transport establishment 1, First positioning region and opposite second positioning region cooperation and microscope carrier 2 is navigated into the corresponding working region (example of microscope carrier transport establishment 1 Such as the first working region A, the second working region B or third working region C), the reliability of positioning of microscope carrier 2 is effectively ensured, from And corresponding sample process operation is executed (such as in the first working region A execution sample dispensing operation, in the second working region B Nucleic acid extraction operation is executed, executes detection of nucleic acids operation in third working region C), the first positioning region and opposite second later Positioning region occur separation and make microscope carrier 2 relative to microscope carrier transport establishment 1 be in move freely state, so that microscope carrier 2 moves again It moves to subsequent work region, and repeatedly by cooperation between the first positioning region as described above and the second positioning region and separation Operation, and then favorably accomplish sample process operation.
Here, the fit system of the first positioning region and the second positioning region can use the arragement construction of various reasonable, for example, It specifically can for example be used electronic when using flexible fit system using fit systems such as flexible, rotation, clampings A variety of stretching structures such as cylinder, hydraulic cylinder or it is also possible to the stretching structure of feed screw nut form, by the flexible of magnetostrictive Structure etc..It for another example, can be by as one of the first positioning region and the second positioning region when use is rotatably assorted mode It rotates the rotation of executive item and is caught in the form of another one to realize the cooperation or separation of the first positioning region and the second positioning region.Again Such as, using fit system is clamped, the second positioning can be clamped by can be used as the clamping piece of the first positioning region The form in portion is realized.
In an embodiment of the disclosure, in order to enable the fit form of the first positioning region and the second positioning region is simple And be easy to arrange on microscope carrier 2 and microscope carrier transport establishment 1, optionally, one of the first positioning region and the second positioning region are formed For locating slot 221 or location hole, another one is formed as extensible member, which is configured to be inserted into and navigates to locating slot It 221 or positioning hole or is detached from from locating slot 221 or location hole.Here, optionally, as shown in Figure 23 and Figure 32, the second positioning Portion is the first magnet plunger 151, and the first positioning region is locating slot 221, and the first magnet plunger 151 has working condition and inoperative shape State, in working condition, the first magnet plunger 151 power-off, so that the push rod of the first magnet plunger 151 stretches out and is adsorbed onto locating slot In 221, in off working state, the first magnet plunger 151 is powered, to make push rod by the magnetic force generated in the first magnet plunger 151 It retracts and is detached from locating slot 221.That is, locating slot 221 is formed on the first positioning seat 22 of microscope carrier 2, by the first magnet plunger 151 It is arranged on the second positioning seat 15 of microscope carrier transport establishment 1, can be convenient due to locating slot 221 and is easily worked into microscope carrier 2 On, so that 2 overall structure of microscope carrier is simple and can move flexibly and easily in microscope carrier transport establishment 1.Wherein, the first electromagnetism Bar 151 is to realize the linear reciprocation of itself push rod using the actuation and release of electromagnet dynamic iron core and static iron core with push rod The extensible member of movement.When being placed exactly in 2 moving process of microscope carrier in multiple first magnet plungers 151 with microscope carrier transport establishment 1 When the corresponding position of one, i.e., in the operating condition, corresponding first magnet plunger 151 powers off, so that push rod is stretched out and is inserted into In locating slot 221, microscope carrier 2 is thus navigated into corresponding working region in microscope carrier transport establishment 1；In the corresponding working region After the interior corresponding sample process operation of completion, i.e., in a non-operative state, the first magnet plunger 151 is powered and is generated by internal Magnetic force and make pusher retracted so that microscope carrier 2 positioning is released from microscope carrier transport establishment 1 and in free movement shape State.This first magnet plunger 151 installation space shared by it on the second positioning seat 15 is small and in the limited of microscope carrier transport establishment 1 Multiple first magnet plungers 151 can be reasonably arranged in space, and have many advantages, such as that maintenance cost is low, reliable for operation.But this public affairs Open that it's not limited to that, it, can be using structures such as electric pushrods as the alternative of the first magnet plunger 151.
Optionally, the second positioning seat 15 is separately provided for detection first on the position of each second positioning region The position sensor of the position of positioning seat 22 detects the first positioning with a position sensor in multiple position sensors When the position of seat 22, so that corresponding to the second positioning region of a position sensor and first positioning region in the second positioning region Cooperation.Here, position sensor can use various structures, such as edge carries in microscope carrier transport establishment 1 in order to not influence microscope carrier 2 The movement of platform moving direction Z1, position sensor can be non-contact position sensor, such as optionally, position sensor is Photoelectrical position sensor, the Mechanical Contact that this photoelectrical position sensor eliminates between microscope carrier 2 and microscope carrier transport establishment 1 are asked Topic avoids that movement interference occurs to microscope carrier 2, and also has the advantages that service life length, high reliablity.Here, optionally, extremely such as Figure 22 Shown in Figure 26, position sensor is groove profile photoelectrical position sensor 16, and the slot of groove profile photoelectrical position sensor 16 is transported towards microscope carrier The inside opening of transfer mechanism 1 is formed with the slot for passing through groove profile photoelectrical position sensor 16 on first positioning seat 22 Block 222.Here, the slot of groove profile photoelectrical position sensor 16 can be U-lag, transmitter and receiver are located at U-shaped The two sides of slot simultaneously form optical axis, microscope carrier 2 in microscope carrier transport establishment 1 along microscope carrier moving direction Z1 motion process, when microscope carrier 2 The first positioning seat 22 on block 222 by between transmitter and receiver and when blocking optical axis, groove profile optoelectronic position sensing Device 16 generates the on-off model for detecting block 222, according to the signal that trough type photoelectric sensor 16 detects, microscope carrier transporter It is located on structure 1 and matches with the second positioning region of 16 corresponding position of groove profile photoelectrical position sensor with the first positioning region on microscope carrier 2 It closes, so that microscope carrier 2 is navigated to the corresponding working region of microscope carrier transport establishment 1, here, the second positioning region is as described above In the case that first magnet plunger 151 and the first positioning region are locating slot 221, the first magnet plunger 151 powers off and push rod is stretched out And be inserted into locating slot 221, thus reliable location microscope carrier 2.But it's not limited to that for the disclosure, and position sensor can also be adopted With other structures, for example, position sensor can be magnetic sensitive position sensor, this can equally eliminate microscope carrier 2 and microscope carrier fortune The Mechanical Contact of transfer mechanism 1, and reliability is higher.
On the basis of microscope carrier location structure as described above, for the specific of the first positioning seat 22 and the second positioning seat 15 Installation site can select according to the actual situation, for example, in order to enable microscope carrier 2 and the fit structure of microscope carrier transport establishment 1 more Adduction reason and realization reliable location, optionally, the first positioning seat 22 is arranged on the side wall of microscope carrier 2, the setting of the second positioning seat 15 On the side inner sidewall of microscope carrier transport establishment 1.
Here, can by movable trolley 122 as described above according to the microscope carrier 2 of first embodiment as described above It is movably arranged in microscope carrier transport establishment 1 along microscope carrier moving direction Z1.Here, driving microscope carrier 2 to exist by movable trolley 122 It is moved in microscope carrier transport establishment 1 along microscope carrier moving direction Z1, and passes through the first positioning region of microscope carrier 2 and microscope carrier transport establishment 1 On the second positioning region detection to 2 position of microscope carrier of cooperation and position sensor, so that microscope carrier 2 is navigated to microscope carrier transporter Structure 1 executes correspondingly sample process operation to areas inside operating, and the control precision to microscope carrier 2 has thus been effectively ensured.
Further optionally, described according to the second embodiment of the disclosure as shown in Figure 22 to Figure 27, Figure 33 to Figure 36 Cooperation assembly includes the microscope carrier guide frame for being oriented to movement of the microscope carrier 2 in microscope carrier transport establishment 1, microscope carrier guide frame packet It includes the microscope carrier guide rail 17 of 1 two sides of microscope carrier transport establishment and slidably engages and be arranged in 2 bottom two of microscope carrier with microscope carrier guide rail 17 The two sides being located on microscope carrier moving direction Z1 in microscope carrier transport establishment 1 are arranged in the first pulley component 23 of side, microscope carrier guide rail 17 On inner wall.Here, microscope carrier guide frame may include the microscope carrier 2 such as flowering structure.
That is, for microscope carrier guide frame microscope carrier 2 may include for in microscope carrier transport establishment 1 be located at microscope carrier movement side The first pulley component 23 that microscope carrier guide rail 17 on two sides inner wall on Z1 cooperates, first pulley component 23 are arranged in microscope carrier 2 two sides of the bottom, the moving along microscope carrier moving direction Z1 on microscope carrier guide rail 17 of microscope carrier 2, each first pulley group can be oriented to Part 23 has two groups of first pulleys at the interval X2 in transverse direction, and the wheel shaft of each first pulley is vertically disposed at microscope carrier 2 On, two groups of first pulleys of each first pulley component 23 being capable of X2 adjustment two groups the in transverse direction by position adjustment structure Horizontal spacing between the wheel shaft of one pulley, wherein transverse direction X2 is arranged perpendicularly to microscope carrier moving direction Z1.Here, position The arragement construction of various reasonable can be used by setting adjustment structure, as long as can be realized two groups of first pulleys of adjustment X2 in transverse direction Adjust the function of spacing.It wherein, can be by making any one group first in two groups in each first pulley component 23 Pulley adjusts position along the direction close to or far from another group of first pulley on transverse direction X2, or can also be by making Two groups of first pulleys adjust position along direction close to each other or separate on transverse direction X2, so as to adjust two groups the Thus microscope carrier 2 is being installed under the use state in transport establishment 1, can effectively ensure that two groups by the spacing between one pulley First pulley is bonded with the two sides inner wall of the microscope carrier guide rail 17 of microscope carrier transport establishment 1, plays reliable guiding microscope carrier 2 in transporter Movement on structure 1, meanwhile, the spacing between two groups of first pulleys is adjusted by position adjustment structure, it can be from a variety of different knots Thus the cooperation of microscope carrier guide rail 17 of structure, various sizes of microscope carrier transport establishment 1 has extensive versatility, mass may be implemented It produces and reduces the exploitation and manufacturing cost that manufacture first pulley component 23 for the microscope carrier guide rail 17 of different structure.In addition, First pulley component 23 can pass through subsequent adjustment first pulley component 23 because long-time service causes in the case where wearing Lateral position compensates abrasion loss without replacing first pulley component 23, thereby saves microscope carrier 2 and microscope carrier guide frame Maintenance cost and the recycling property for improving first pulley component 23.
Here, optionally, such as scheming to quickly and easily adjust the horizontal spacing between the wheel shaft of two groups of first pulleys Shown in 36, two groups of first pulleys are respectively that the first fixed pulley 232 and the first movable pulley 233, the first movable pulley 233 pass through Position adjustment structure can adjust position by X2 in transverse direction, and two limit adjustment position of the first movable pulley 233 is located at The two sides of first fixed pulley 232.As a result, only by adjusting the first movable pulley 233 on the bottom of microscope carrier 2 in transverse direction The position of X2 enables to the first movable pulley 233 and the first fixed pulley 232 to paste with the two sides inner wall of microscope carrier guide rail 17 It closes, while the position of the first movable pulley 233 can also be adjusted according to actual needs, be located at the first fixed pulley 232 Left or right side, or arrange structure in a row with the first fixed pulley 232, so as to adjust two sides first pulley component 23 it Between spacing.It specifically may be adjusted so that the spacing between two sides first pulley component 23 becomes smaller, also adjustable is to make Spacing between two sides first pulley component 23 becomes larger, allow first pulley component 23 the bottom of microscope carrier 2 neatly It realizes adjustment, and then can adapt to the microscope carrier guide rail 17 of a variety of different spacing.
In order to enable the structure of first pulley component 23 is more rationalized and can be led more stablely on microscope carrier guide rail 17 Movement to microscope carrier 2, optionally, the first fixed pulley 232 and the first movable pulley 233 are multiple, and respectively along microscope carrier 2 Longitudinal direction X1 arrangement, longitudinal direction X1 are aligned parallel to microscope carrier moving direction Z1, the first fixed pulley 232 and the first activity Pulley 233 is alternately arranged on longitudinal direction X1.Wherein, multiple first movable pulleys 233 can by connect lever linkage, by This can pass through connecting rod in the case where adjusting the horizontal spacing between the first movable pulley 233 and the first fixed pulley 232 Design multiple first movable pulleys 233 of synchronous adjustment position, thus improve the first movable pulley 233 adjustment position effect Rate improves operation ease.
Here, in order to enable microscope carrier overall structure is simpler, molding easy to process, optionally, such as Figure 36 and Figure 37 institute Show, first pulley component 23 is mounted on the bottom of microscope carrier 2 by first pulley mounting base 231, and position adjustment structure includes being formed First pulley in first pulley mounting base 231 installs block hole 234, and the be arranged on the first movable pulley 233 The wheel shaft 2331 of one pulley fastening structure, the first movable pulley 233 can be inserted into first with adjusting position by X2 in transverse direction Pulley is installed in block hole 234, and is positioned by first pulley fastening structure.Here, first pulley fastening structure can use Various structures, as long as can be realized in first pulley installation block hole 234, X2 adjusts position and enables in transverse direction First movable pulley 233 is relatively fixed to the function of the bottom of microscope carrier 2.Optionally, as shown in figure 38, first pulley fastens Structure include the screw thread 235 being formed on 2331 outer peripheral surface of wheel shaft of the first movable pulley 233 and have connect with screw thread 235 Internal screw thread fastening nut 236, be formed in first pulley mounting base 231 be connected to first pulley installation block hole 234 and For accommodating the fastening nut mounting groove 238 of fastening nut 236, the fastening nut 236 is formed as cylindrical body and the interior spiral shell 2361 arranged off-centre of line is on the fastening nut 236.Wherein, it can be set between the first movable pulley 233 and wheel shaft 2331 There is the first movable pulley bearing 2332.It, can be first loose from fastening nut 236 when adjusting the position of the first movable pulley 233 The wheel shaft 2331 for opening the first movable pulley 233 enables the wheel shaft 2331 of the first movable pulley 233 in first pulley mounting base Movement in through-hole 234 specifically makes the wheel shaft 2331 connecting with the internal screw thread of bias the by rotating adjusting nut 236 One pulley installs movement in block hole 234, to adjust the position of the first movable pulley 233 on transverse direction X2, is being adjusted to The internal screw thread 2361 of fastening nut 236 and the screw thread 235 of wheel shaft 2331 are tightened again behind position, it thus can by the first movable pulley 233 The bottom of microscope carrier 2 is locked at by ground, so that it is more simple to adjust operation.But it's not limited to that for the disclosure, first pulley component 23 can also use other reasonable structures, for example, the first movable pulley 233 is configured to screw bolt-type pulley, at this point, first Pulley mounting base could be formed with the threaded hole being threadedly coupled with the screw thread of the screw bolt-type pulley, and this structure equally also can Enough realize the function that the first movable pulley 233 is fastened after the position of the first movable pulley 233 adjusts.
Optionally, it as shown in Figure 30 and Figure 37, is circumferentially formed on the outer peripheral surface of first pulley for being inserted into and cooperating Microscope carrier guide rail mating groove 237 on to the microscope carrier guide rail 17 of microscope carrier transport establishment 1.Herein optionally, the load of microscope carrier transport establishment 1 Platform guide rail 17 can be formed as being formed with both ends open along guide rail extending direction at the top of microscope carrier guide rail 17 such as flowering structure Microscope carrier mating groove 171 is formed with opposite microscope carrier limit outstanding along guide rail extending direction on the two sides inner wall of microscope carrier mating groove 171 Position protrusion 172, microscope carrier guide rail mating groove 237 is formed as the shape cooperated with microscope carrier retention bead 172.Here, for example, microscope carrier limits Position protrusion 172 is formed as semicylinder shape, at this point, microscope carrier guide rail mating groove 237 is formed as cooperating with microscope carrier retention bead 172 Semicircular arc groove, that is, first pulley can use concave groove pulley.As a result, in first pulley component 23 from microscope carrier guide rail When 17 open at one end is installed on microscope carrier guide rail 17, pass through the cooperation of microscope carrier mating groove 171 and microscope carrier retention bead 172, energy It enough effectively prevent first pulley component 23 to be detached from from microscope carrier guide rail 17, while also acting as the effect of certain supporting platform 2.But this Open it's not limited to that, and first pulley can be formed as other structures, for example, first pulley is configured to common flat table Face type pulley etc..
Optionally, it is provided on microscope carrier 2 for microscope carrier 2 to be navigated to each working region in microscope carrier transport establishment 1 Microscope carrier positioning region.Here, microscope carrier positioning region can be using the structure of the first following positioning seat, by transporting with following microscope carrier The cooperation of the second positioning seat in mechanism 1 and make microscope carrier 2 be accurately positioned in each working region of microscope carrier transport establishment 1 and Corresponding sample process operation is executed, the reliability of positioning to microscope carrier 2 has been effectively ensured.
To sum up, the microscope carrier guide frame of the microscope carrier for being provided with first pulley component including second embodiment as described above All features and function and effect as described above comprising being provided with the microscope carrier 2 of first pulley component, herein in order to avoid repeating, Details are not described herein.
It is described with reference to as shown in Figure 32 to Figure 35 and Figure 39 to Figure 42 in addition, according to the third embodiment of the disclosure Microscope carrier and the cooperation assembly of microscope carrier transport establishment may include following microscope carrier.That is, microscope carrier 2 may include fixing seat 24 and can stretch The sliding seat 25 in fixing seat 24 is arranged in contracting ground, and the container for accommodating sample container 3 is provided in sliding seat 25 and is accommodated Slot 251.As a result, by microscope carrier 2 as above, under the use state cooperation assembly as described above, fixing seat 24 is installed It, herein can be by making sliding seat 25 relative to the expanding-contracting action of fixing seat 24, so that sliding seat 25 in microscope carrier transport establishment 1 On container holding tank 251 relative to microscope carrier transport establishment 1 expose and convenient for execute sample container 3 pick-and-place movement, thus Improve the pick-and-place efficiency and speed of sample container.
Here, the structure of various reasonable can be used for the fit structure between sliding seat 25 and fixing seat 24, as long as Sliding seat 25 is enabled to make container holding tank 251 be exposed to microscope carrier transport establishment 1 relative to 24 relative motion of fixing seat Outside.For example, optionally, microscope carrier 2 includes using in order to reliably be oriented to expanding-contracting action of the sliding seat 25 in fixing seat 24 In the sliding seat guidance set 26 that guiding sliding seat 25 is slided along the first telescopic direction P relative to fixing seat 24.Here, sliding seat 25 the first telescopic direction P can be used perpendicular to microscope carrier moving direction Z1, sliding seat guidance set 26 and be slidably matched, roll The modes such as dynamic cooperation, for example, optionally, sliding seat guidance set 26 may include mutual using being slidably matched The guide groove 261 and guide protrusions 262 of cooperation, guide groove 261 are arranged in sliding seat 25 and fixing seat 24 on any one, And extend along the first telescopic direction P, guide protrusions 262 are arranged in sliding seat 25 and fixing seat 24 in remaining one.For example, such as Shown in Figure 35, it can be formed with guide groove 261 in the two sides of fixing seat 24, accordingly, the two sides of sliding seat 25 are formed with and lead The guide protrusions 262 cooperated to slot 261 are played by the cooperation of guide protrusions 262 and guide groove 261 stablize guiding work as a result, Dynamic expanding-contracting action of the seat 25 in fixing seat 24.For another example, it in the case that sliding seat guidance set 26 is using cooperation is rolled, can adopt With such as flowering structure.That is, sliding seat guidance set 26 may include that the scroll wheel guide rail that 24 two sides of fixing seat are arranged in and setting exist The two sides of sliding seat 25 and the scroll wheel that cooperation is rolled with scroll wheel guide rail, as a result, through scroll wheel on scroll wheel guide rail It rolls so that sliding seat 25 neatly executes expanding-contracting action in fixing seat 24.But the disclosure is not limited to above-mentioned two implementation Mode, sliding seat guidance set 26 can use other reasonable arragement constructions, as long as can be realized reliable guiding sliding seat 25 Flexible function in fixing seat 24.
Optionally, as shown in Figure 33 and Figure 40, microscope carrier 2 includes the shape being fully retracted into sliding seat 25 relative to fixing seat 24 For limiting the sliding seat limit assembly of sliding seat 25 under state.That is, after sliding seat 25 is fully retracted in fixing seat 24, it should Sliding seat limit assembly can play position-limiting action to sliding seat 25 so that sliding seat 25 and fixing seat 24 it is relatively fixed and in sample Structure moves in microscope carrier transport establishment 1 as a whole during present treatment, for example, can by with above-mentioned movable trolley 122 Cooperation and moved in microscope carrier transport establishment 1 along microscope carrier moving direction Z1, thus in addition to execute pick and place operation when make sliding seat Except 25 stretch relative to fixing seat 24, microscope carrier 2 sliding seat 25 and fixing seat 24 during the entire process of sample process are protected always Hold relatively fixed, guarantee sample process operation normal operation.Here, optionally, sliding seat limit assembly is limit latch components, For example, being can be set using latch components etc. usually used on drawer, this latch components in sliding seat 25 and be fixed The bottom position of seat 24.Alternatively, alternatively, optionally, sliding seat limit assembly includes opposite two of magnetic force Magnetic absorption member 27, two magnetic absorption members 27 are separately positioned on the opposing sidewalls of fixing seat 24 and sliding seat 25 on the first telescopic direction P On.That is, two magnetic absorption members 27 are separately positioned on the most inboard inner wall along the first telescopic direction P of sliding seat 25 and fixing seat 24 On, wherein two magnetic absorption members 27 can use the permanent magnet of simple structure, be fully retracted in fixing seat 24 in sliding seat 25 When, two magnetic absorption members are mutually attached together and that sliding seat 25 and fixing seat 24 are reliably fixed is integral.
Optionally, container holding tank 251 is disposed in sliding seat 25 along the first compartment of terrain telescopic direction P multiple, thus Multiple sample containers 3 in container holding tank 251 are being loaded by a microscope carrier 2 while being moved to corresponding workspace It is synchronized in domain and executes corresponding sample process operation, improve whole sample process efficiency.Further optionally, each container holds Receive and be provided with mistake proofing assembling structure 252 on slot 251, in a state of use can portion corresponding with the anti-misloading of sample container 3 match It closes and sample container 3 is correctly installed in container holding tank 251 along nucleic acid extraction direction Z2.Here, mistake proofing assembling structure 252 can be according to the specific structure for the sample container 3 being put into container holding tank 251 come reasonable design, such as sample The bottom of container 3 could be formed with the anti-misloading gap that portion is corresponded to as anti-misloading, in the case, mistake proofing assembling structure 252 Can be formed as the structures such as the convex rib being inserted correspondingly into anti-misloading gap, more specifically, for example, as shown in figure 41, it is convex Playing muscle structure can accommodate along the first telescopic direction P across the opposite two sides and deviation container for being arranged in container holding tank 251 Slot 251 is along the side of nucleic acid extraction direction Z2, to adapt to the correspondence of sample container 3 in the case where preventing sample container 3 The arragement construction of holding tank, by above structure, when sample container 3 is put into the poor direction of container holding tank 251, It cannot achieve depositing for sample container 3 since the anti-misloading portion of correspondence of mistake proofing assembling structure 252 and sample container 3 is not corresponding It puts.
In order to enable the fit structure of fixing seat 24 and sliding seat 25 is simple and more rationalizes, optionally, such as Figure 39 and Shown in Figure 40, the top of fixing seat 24 for open architecture and including fixing seat bottom plate 241 and surrounds 241 edge of fixing seat bottom plate The fixing seat side wall 242 of arrangement, to be formed between fixing seat bottom plate 241 and fixing seat side wall 242 for accommodating sliding seat 25 Sliding seat accommodating chamber 243, fixing seat side wall 242 has opening so that sliding seat 25 is flexible in the side of the first telescopic direction P. Optionally, as shown in Figure 41 and Figure 42, sliding seat 25 includes sliding seat pedestal 253 and is arranged at the top of sliding seat pedestal 253 Sliding seat top plate 254, sliding seat pedestal 253 and sliding seat top plate 254 cooperate and are formed with container holding tank 251, container The opening of holding tank 251 is formed on sliding seat top plate 254.Here, guaranteeing the branch of sliding seat 25 while in order to save material The intensity of sample container 3 is supportted, sliding seat pedestal 253 can be formed as rectangular plate body up and down, sliding seat top plate 254 It is covered on the top of sliding seat pedestal 253, here, sliding seat top plate 254 and sliding seat pedestal 253 can be fastened by bolt etc. Part is connected as one, and in the case where sample container 3 is stored in microscope carrier 2, the part-structure of sample container 3 can be from activity The opening of the container container 251 of seat top plate 254 is exposed, and another part structure is then located at sliding seat top plate 254 and sliding seat bottom In the internal cavities that seat 253 is constituted, so that storage has the microscope carrier 2 of sample container 3 is whole to maintain suitable height, with The movement being easy to implement in microscope carrier transport establishment 1.But it's not limited to that for the disclosure, can root for the specific structure of microscope carrier 2 It is reasonably arranged according to actual needs, as long as enabling to sliding seat 25 flexible relative to fixing seat 24.
Optionally, the top of fixing seat side wall 242 protrudes from the top surface of sliding seat top plate 254, and fixing seat side wall 242 Top is provided with towards sliding seat accommodating chamber 243 outstanding anti-detachment raised 244, this anti-detachment raised 244 with sliding seat top plate There is gap between 254 top surface, prevent sample container 3 de- can cooperate in a state of use with sample container 3 From container holding tank 251.That is, after sliding seat 25 is put into sample container 3 relative to the stretching of fixing seat 24, in sliding seat During 25 retract relative to fixing seat 24, the part that sample container 3 is exposed to sliding seat top plate 254 can be inserted into anticreep It contacts from the gap between protrusion 244 and sliding seat top plate 254 and with anti-detachment raised 244 bottom surface, is thus moved in microscope carrier 2 Sample container 3 can be effectively prevent to be detached from from microscope carrier 2 in the process.
To sum up, the cooperation assembly of the microscope carrier including third embodiment as described above is by being assembled to microscope carrier for fixing seat 24 When in transport establishment 1, it can use sliding seat 25 and stretched relative to fixing seat 24 (in other words relative to microscope carrier transport establishment 1) Contracting movement, so that the container holding tank 251 in sliding seat 25 exposes relative to microscope carrier transport establishment 1, in order to hold The pick-and-place of row sample container 3 acts, and thus improves the pick-and-place efficiency and speed of sample container 3, and then improve the cooperation The operating efficiency of assembly and sample processing device.
Above-mentioned first discloses microscope carrier and microscope carrier transport establishment with different structure feature into third embodiment, This is it should be noted that may include as described above three in sample processing device and microscope carrier and the cooperation assembly of microscope carrier transport establishment The microscope carrier of any one or more in kind embodiment and microscope carrier transport establishment, for example, first embodiment can be only included It is provided with the microscope carrier of the first positioning seat in microscope carrier location structure and is provided with the microscope carrier transport establishment of the second positioning seat, or can be with It is provided with the microscope carrier of first pulley component in microscope carrier guide frame including second embodiment and is provided with the load of microscope carrier guide rail Platform transport establishment, or may include third embodiment including the fixing seat 24 that is mounted in microscope carrier transport establishment and be used for Sample container 3 is accommodated and the microscope carrier of the sliding seat 25 flexible relative to fixing seat 24, to pass through work in microscope carrier transport establishment Stretching or the retract action of dynamic seat 25 and convenient for picking and placing sample container 3.Or the cooperation assembly may include above-mentioned three It any two or three in kind embodiment, using any two or three, is set respectively on same microscope carrier Set corresponding structure, i.e., suitable for cooperate the microscope carrier assembly can include simultaneously the first positioning seat, first pulley component and Any two or three in fixing seat and sliding seat.In the sample processing device of the disclosure, in order to understand and completely say Structure, working principle and the processing method of the sample processing device of the bright disclosure, it includes such as that sample processing device, which uses, The microscope carrier of the corresponding construction of upper three kinds of embodiments and microscope carrier transport establishment.
Secondly, total to the cooperation that can be suitable for referring to as described above referring to Fig. 1 to Figure 11 and Figure 22 to Figure 30 It is described in detail at structure, feature and the function and effect of the microscope carrier transport establishment with sample processing device.
The microscope carrier transport establishment 1 of the disclosure may include the multilayer microscope carrier shipping platform 11 along height direction H arrangement, The working region for handling sample is disposed on each layer microscope carrier shipping platform 11, at least one of microscope carrier shipping platform 11 It is provided with microscope carrier driven in translation structure 12, the microscope carrier driven in translation structure 12 is for driving microscope carrier 2 flat in the transport of corresponding microscope carrier Position is adjusted along microscope carrier moving direction Z1 in the working region of platform 11, at least one upper setting of remaining in microscope carrier shipping platform 11 There is microscope carrier to go up and down driving structure 13, microscope carrier lifting driving structure 13 is used to that microscope carrier 2 to be driven to go up and down along height direction H, so that The switching position between each layer microscope carrier shipping platform 11 of microscope carrier 2.That is, being put down since microscope carrier transport establishment is provided with the transport of multilayer microscope carrier Platform 11, each working region for handling sample rationally can hierarchically be arranged in multilayer microscope carrier shipping platform 11, at this time Driving structure 13 can be gone up and down by microscope carrier drives microscope carrier 2 to go up and down between multilayer microscope carrier shipping platform 11 along height direction H Switching freely, and can drive microscope carrier 2 on microscope carrier shipping platform 11 along the microscope carrier side of movement by microscope carrier driven in translation structure 12 It is mobile to Z1, so that microscope carrier 2 adjusts position neatly on multilayer microscope carrier shipping platform 11 to navigate to each workspace On domain.Here, the microscope carrier transport establishment for being suitable for sample processing device can be disclosed microscope carrier transport establishment as described above, That is, the microscope carrier transport establishment 1 includes the multilayer microscope carrier shipping platform 11 along height direction H arrangement, at this point, refer among the above the One working region A, the second working region B and third working region C can be arranged in any in multilayer microscope carrier shipping platform 11 In one, microscope carrier 2 is moved on microscope carrier shipping platform 11 by microscope carrier driving mechanism, and microscope carrier driving mechanism includes microscope carrier translation Driving structure 12 and microscope carrier go up and down driving structure 13, and microscope carrier driven in translation structure 12 is arranged in microscope carrier shipping platform 11 at least In one, and for driving microscope carrier 2 to adjust in the working region of corresponding microscope carrier shipping platform 11 along microscope carrier moving direction Z1 Position, microscope carrier lifting driving structure 13 be arranged in microscope carrier shipping platform 11 remaining at least one on, and for driving microscope carrier 2 It is gone up and down along height direction H, so that the switching position between each layer microscope carrier shipping platform 11 of microscope carrier 2.From there through as described above Microscope carrier transport establishment and sample processing device including the microscope carrier transport establishment, enable to multiple working regions to realize three-dimensional Arrangement, saves the required arrangement areas of sample processing device, and make multiple working region characteristics of compact layout, improves microscope carrier translation and drives Dynamic structure 12 and microscope carrier go up and down driving structure 13 for the drive efficiency of microscope carrier 2, and then can be improved sample process efficiency.
Here, microscope carrier driven in translation structure 12 as described above can be the specific implementation disclosed in above-mentioned cooperation assembly Microscope carrier driven in translation structure 12 in mode omits its description herein to avoid repeating.
Optionally, as shown in figure 22, microscope carrier shipping platform 11 includes the upper layer microscope carrier fortune arranged along height direction H perforation Defeated platform 111 and lower layer's microscope carrier shipping platform 112.As a result, microscope carrier transport establishment 1 can be realized the premise of three-dimensional arrangement Under, weight is reduced by penetrating through the structure of the upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 that are arranged and is use up The overall structure of microscope carrier transport establishment 1 may be simplified, meanwhile, have convenient for arrangement microscope carrier driven in translation structure 12 and microscope carrier liter The effect of driving structure 13 is dropped, and is also convenient for driving microscope carrier 2 and is moved in microscope carrier transport establishment 1.Here, microscope carrier lifting driving knot Structure 13 can be arranged in the perforation region of microscope carrier transport establishment 1, such as can be arranged under lower layer's microscope carrier shipping platform 112 The region Fang Guantong, it is dry in inoperative movement will not be generated to other movable parts such as microscope carrier 2, microscope carrier driven in translation structure 12 Relate to phenomenon.Wherein, microscope carrier guide rail 17 as described above is separately positioned on upper layer microscope carrier shipping platform 111 and the transport of lower layer's microscope carrier is flat On platform 112, so that microscope carrier 2 moves on upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 along microscope carrier respectively Direction Z1 is smoothly moved.Here, for the arrangement volume cost of microscope carrier driven in translation structure 12 and microscope carrier lifting driving structure 13 Open not limit this, condition needed for being handled according to actual sample is come reasonable design.For example, microscope carrier driven in translation knot Structure 12 can be positioned only on upper layer microscope carrier shipping platform 111 or lower layer's microscope carrier shipping platform 112, or be arranged at upper layer load On platform shipping platform 111 and lower layer's microscope carrier shipping platform 112, microscope carrier lifting driving structure 13 can be set transports in lower layer's microscope carrier On the one or both ends position of platform 112.In addition, in order to steadily support upper layer microscope carrier shipping platform 111 and lower layer to carry Platform shipping platform 112, and guarantee that microscope carrier 2 can work normally in each working region, it is transported by setting in lower layer's microscope carrier The braced frame 19 of the lower part of platform 112 realizes secure support upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112。
It is optional in the case where microscope carrier shipping platform 11 as described above is suitable for microscope carrier processing equipment as described above again Ground, as shown in Figures 4 to 6, the first working region A, the second working region B and third working region C are along microscope carrier moving direction Z1 is sequentially arranged on upper layer microscope carrier shipping platform 111, and microscope carrier driven in translation structure 12 is arranged in upper layer microscope carrier shipping platform 111 On, microscope carrier goes up and down driving structure 13 and is arranged on lower layer's microscope carrier shipping platform 112, and the working region further includes for storing At least part region of the initial storage area D of the microscope carrier of multiple microscope carriers 2, the initial storage area D of microscope carrier are arranged in lower layer's microscope carrier On shipping platform 112.Here, a part of region of the initial storage area D of microscope carrier is arranged for example as shown in Fig. 5, Fig. 6 and Figure 11 On lower layer's microscope carrier shipping platform 112, remainder region is arranged on upper layer microscope carrier shipping platform 111, herein for the ease of It arranges microscope carrier 2 and microscope carrier 2 is left concentratedly together, the initial storage area D of microscope carrier is located at 111 He of upper layer microscope carrier shipping platform The position close to the first working region A of lower layer's microscope carrier shipping platform 112.In addition, being carried in the specific embodiment of the disclosure There are six platform 2 can be set, wherein three microscope carriers 2 can be arranged in the initial storage area of microscope carrier of upper layer microscope carrier shipping platform 111 Domain D, excess-three microscope carrier 2 can be arranged in the initial storage area D of microscope carrier of lower layer's microscope carrier shipping platform 112.But the disclosure is simultaneously It is not limited to this, for example, the initial storage area D of microscope carrier can be all arranged on lower layer's microscope carrier shipping platform 112.
Here, by arranging the first working region A, the second working region B and on upper layer microscope carrier shipping platform 111 Three working region C, therefore, the microscope carrier driven in translation structure 12 for driving microscope carrier 2 to move along microscope carrier moving direction Z1 can be only Be arranged on upper layer microscope carrier shipping platform 111, herein for ease of control microscope carrier driven in translation structure 12 operation and make The overall structure of microscope carrier transport establishment 1 and sample processing device is simplified, and microscope carrier driven in translation structure 12 can only be provided with one It is a, i.e., all moving along microscope carrier moving direction Z1 of microscope carrier 2 are driven by a microscope carrier driven in translation structure 12.Here, can Selection of land, microscope carrier driven in translation structure 12 are set as, and can drive microscope carrier 2 in the second working region B along in contrast to the microscope carrier side of movement It is advanced to the direction of the separate third working region C of Z1 with step-by-step movement, with can be by mobile with the pipette tips of nucleic acid extraction mechanism 4 The sample in sample container 3 is extracted in the cooperation of the expanding-contracting action of the lifting action and magnetic bead transfer device 42 of device 41 Contained in nucleic acid and the nucleic acid of extraction is stored to each sample container 3.Here, in order to clearly illustrate microscope carrier driven in translation Structure 12 drives the mode of microscope carrier 2 in the second working region B, on the basis of microscope carrier moving direction Z1, is located at microscope carrier movement side It is defined as rear side to the upstream side Z1, is defined as front side, the first working region A and third positioned at the downstream side microscope carrier moving direction Z1 Working region C is located at rear side and the front side of the second working region B, and in the case, microscope carrier is flat in the second working region B Moving driving structure 12 drives microscope carrier 2 to carry out step-by-step movement backward movement towards the rear side in contrast to microscope carrier moving direction Z1, thus leads to The cooperation with the pipette tips mobile device 41 of nucleic acid extraction mechanism 4 and magnetic bead transfer device 42 is crossed, the nucleic acid of extraction is stored to sample In the sample holding tank of 3 front side of this container.Later by microscope carrier driven in translation structure 12 will complete the microscope carrier 2 of nucleic acid extraction from Second working region B is moved to third working region C along microscope carrier moving direction Z1, at this point, due to the nucleic acid storage extracted In the sample holding tank of 3 forward position of sample container, so that second mechanical arm is by the way of smaller movement routine The nucleic acid quickly and easily is dispensed into multiple deep-well plates 100, further improves sample process operating efficiency.But this public affairs Open that it's not limited to that, microscope carrier driven in translation structure 12 may be set to be, and microscope carrier 2 can be driven on the second working region edge B Microscope carrier moving direction Z1 is advanced with step-by-step movement, and in the case, cell pyrolysis liquid holding tank, protein digestibility enzyme solutions accommodate Slot, cleaning solution holding tank, eluent holding tank and nucleic acid holding tank are on sample container along in contrast to microscope carrier moving direction Z1 Direction be sequentially arranged, the nucleic acid extracted is then placed in the nucleic acid holding tank positioned at rear side of sample container 3.
When executing sample process operation, microscope carrier 2 can be driven in upper layer microscope carrier by microscope carrier driven in translation structure 12 The second working region B and third working region C are successively moved to from the first working region A on shipping platform 111 and execute sample Operation is handled, here, not limiting this for microscope carrier driven in translation structure 12 disclosure such as the driving sequences of each microscope carrier 2 It is fixed, if can and reasonably driving each microscope carrier 2 respective accordingly completion sample process operation effectiveness.For example, In order to save the whole microscope carrier processing required time, microscope carrier driven in translation structure 12 can use and alternately drive each microscope carrier 2 It is moved to the mode of corresponding working region, this is described in detail subsequent.In addition, in upper layer microscope carrier shipping platform The microscope carrier 2 that entire sample process operation is completed on 111 can be moved to microscope carrier by the lifting that microscope carrier goes up and down driving structure 13 Initial storage area D can also be placed directly into lower layer's microscope carrier for example, the original initial placement position of microscope carrier 2 can be moved to On shipping platform 112, this disclosure is not particularly limited.By structure as described above, sample processing device can be Most sample process operation is executed on upper layer microscope carrier shipping platform 111, lower layer's microscope carrier shipping platform 112 is mainly used to arrange The initial storage area D of microscope carrier for storing microscope carrier 2, so that upper layer microscope carrier shipping platform 111 of the microscope carrier 2 in three-dimensional arrangement Corresponding operation is clearly executed with respectively dividing the work on lower layer microscope carrier shipping platform 112, and then effectively improves sample process operation effect Rate.
Optionally, as shown in figure 11, microscope carrier driven in translation structure 12 is arranged in the side of upper layer microscope carrier shipping platform 111 On wall, and including the first driving motor 121, the first transmission component being connected with the transmission of the output shaft of first driving motor 121, And the movable trolley 122 that connect and can separatably cooperate with microscope carrier 2 with the first transmission component, the first transmission component are used for The rotary motion of first driving motor 121 is converted to the linear movement of movable trolley 122, to enable to movable trolley 122 Working region locating for microscope carrier 2 is adjusted by the cooperation with microscope carrier 2.Here, can be adopted for microscope carrier driven in translation structure 12 With the arragement construction of various reasonable, for example, microscope carrier driven in translation structure 12 can be the microscope carrier that refers in cooperation assembly as above Driven in translation structure, therefore there is construction identical with the microscope carrier driven in translation structure in above-mentioned cooperation assembly, feature and work With effect, for example, optionally, the first transmission component is that V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair pass Dynamic matching mechanism, optionally, the first transmission component are V belt translation matching mechanism, and including the first transmission belt 123, setting first 123 side of transmission belt and with the first driving wheel 124 of the output axis connection of the first driving motor 121 and setting first transmission The first driven wheel 125 with 123 other sides, movable trolley 122 are mounted on the first transmission belt 123.These technical characteristics, correlation Explain, expansion scheme and generated technical effect refer in the related content part of above-mentioned cooperation assembly, herein for It avoids repeating to repeat no more.
Optionally, as shown in Figure 25 to Figure 30, the downside of the first transmission belt 123 is located in upper layer microscope carrier shipping platform 111 The trolley guide rail 126 extended along microscope carrier moving direction Z1 is provided in inner wall section, movable trolley 122 is fixed on the first transmission belt In 123 downside band part, the bottom of movable trolley 122 is provided with the second pulley slideably cooperated with trolley guide rail 126 Component 127.Optionally, the bottom of movable trolley 122 is arranged in by second pulley mounting base 128 for second pulley component 127, the Two pulley mounting bases 128 are located at 123 downside of the first transmission belt and are supported on trolley guide rail 126 by second pulley component 127. Here, can be using the first pulley component 23 referred in microscope carrier guide frame as described above for second pulley component 127 Structure, specifically can be with reference to the structure of first pulley component 23 shown in Figure 35 to Figure 38, second pulley in the case The relevant technologies feature, explanation, expansion scheme and generated technical effect of component 127 etc. can be oriented to above-mentioned microscope carrier and tie The relevant technologies feature of the first pulley component 23 referred in structure, explanation, expansion scheme and generated technical effect are similar Seemingly, herein in order to avoid its description is omitted in repetition.
Optionally, as shown in figures 30 and 31, it is telescopically provided with stretching structure 8 on movable trolley 122, microscope carrier 2 is right It should be on the side side wall of movable trolley 122, being provided with for cooperating and the locking structure 21 that is mutually locked with stretching structure 8. Movable trolley 122 can successively drive multiple microscope carriers 2 to move by way of the cooperation and disengaging of stretching structure 8 and locking structure 21 It moves to corresponding working region, so that microscope carrier 2 is consecutively carried out sample process operation.Optionally, stretching structure 8 wraps The second magnet plunger 81 is included, the second magnet plunger 81 has lockup state and unlocked state, and in lockup state, the second magnet plunger 81 is disconnected Electricity, so that the push rod of the second magnet plunger 81 is stretched out and snapped on locking structure 21 and locking microscope carrier 2 and movable trolley 122； In unlocked state, the second magnet plunger 81 is powered, unlock pusher retracted by the magnetic force generated in the second magnet plunger 81 Microscope carrier 2 and movable trolley 122, locking structure 21 are formed to have lock groove or locking with the shape of the second magnet plunger 81 cooperation Hole.The relevant technologies feature of stretching structure 8 and locking structure 21, explanation, expansion scheme and generated technology are imitated Fruit etc. refers in the relevant technologies partial content in above-mentioned microscope carrier and the cooperation assembly of microscope carrier transport establishment, herein in order to It avoids repeating to repeat no more.
Optionally, as shown in Figure 22 to Figure 27, Figure 32 and Figure 33, microscope carrier transport establishment includes being arranged in microscope carrier transporter On the side inner sidewall of structure 1 and along the second positioning seat 15 that microscope carrier moving direction Z1 extends, with each for navigating to microscope carrier 2 Working region has been positioned apart from multiple second positioning regions, the second positioning region along microscope carrier moving direction Z1 on the second positioning seat 15 The first positioning region being arranged on the side side wall with microscope carrier 2 cooperates or separates, in lockup state, all second positioning Portion is with the first position portion from so that microscope carrier 2 is in the state of with movable 122 locking of trolley in upper layer microscope carrier shipping platform It is moved freely on 111, in unlocked state, the first positioning region is located at opposite with any one second positioning region in the second positioning region When position, the first positioning region cooperates with corresponding second positioning region, so that microscope carrier 2 is in the state unlocked with movable trolley 122 Under navigate on upper layer microscope carrier shipping platform 111.As described above, upside microscope carrier shipping platform of the microscope carrier 2 in microscope carrier transport establishment 1 On 111 along microscope carrier moving direction Z1 moving process, when the first positioning region is placed exactly in and in microscope carrier transport establishment 1 multiple second When an opposite position in the second positioning region in positioning region, the first positioning region and the second opposite positioning region cooperation and by microscope carrier 2 navigate to the corresponding working region of microscope carrier transport establishment 1 (such as the first working region A, the second working region B or third work Region C etc.), the reliability of positioning of microscope carrier 2 is effectively ensured, thereby executing corresponding sample process operation (such as first work Region A executes sample and dispenses operation, executes nucleic acid extraction operation in the second working region B, executes nucleic acid in third working region C Detect operation), the first positioning region separates with the second opposite positioning region and microscope carrier 2 is transported relative in microscope carrier later Mechanism 1 is in and moves freely state, (such as moves from the initial storage area D of microscope carrier so that microscope carrier 2 is moved to subsequent work region again It moves and is moved to the second working region B to the first working region A, from the first working region A, be moved to from the second working region B Three working region C or be moved to initial storage area D of microscope carrier etc. from third working region C), and pass through as described above the The operation repeatedly of cooperation and separation between one positioning region and the second positioning region, and then favorably accomplish sample process operation.
Optionally, the second positioning region is the first magnet plunger 151, and the first positioning region is locating slot 221, the first magnet plunger 151 With working condition and off working state, in working condition, the first magnet plunger 151 power-off, so that the first magnet plunger 151 pushes away Bar is stretched out and is snapped onto locating slot 221, and in off working state, the first magnet plunger 151 is powered, to pass through the first magnet plunger 151 The magnetic force of interior generation makes pusher retracted and is detached from locating slot 221.Optionally, is provided on the side side wall of microscope carrier 2 One positioning seat 22, locating slot 221 are formed on the first positioning seat 22, and the second positioning seat 15 is adjacent to each first magnet plunger 151 On position, it is separately provided for the position sensor of the position of the first positioning seat 22 of detection, in multiple position sensors Position sensor when detecting the position of first positioning seat 22 so that corresponding in the first magnet plunger 151 described The first magnet plunger and locating slot 221 of one position sensor cooperate.Optionally, position sensor is groove profile optoelectronic position sensing Device 16, the slot of groove profile photoelectrical position sensor 16 towards upper layer microscope carrier shipping platform 111 inside opening, on the first positioning seat 22 It is formed with the block 222 of the slot for passing through groove profile photoelectrical position sensor 16.
It the relevant technologies feature of first positioning region and the second positioning region as described above, explanation, expansion scheme and is produced Raw technical effect etc. refers in the relevant technologies partial content of above-mentioned microscope carrier location structure, herein in order to avoid repeating not It repeats again.
Optionally, referring to figs. 2 to Fig. 4, Fig. 9 and Figure 11, it includes being arranged in lower layer's microscope carrier that microscope carrier, which goes up and down driving structure 13, Lifting fixed pedestal 131 on shipping platform 112 and liftably it is arranged on lifting fixed pedestal 131 and can be with microscope carrier 2 The lift cylinders 132 of cooperation can be detached from so that microscope carrier 2 upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 it Between switching position.Wherein, the bottom of the microscope carrier 2 is provided with for cooperating with lift cylinders 132 can be realized the lifting of lifting Cylinder mating groove, the movable end of lift cylinders 132 are provided with the support base for being used to support microscope carrier, are provided on the support base for being inserted into To the locking projections in the lift cylinders mating groove, enabled to from there through the cooperation of lift cylinders mating groove and locking projections Microscope carrier 2 steadily realizes lifting.Here, one or two has can be set in microscope carrier lifting driving structure 13.For example, being arranged There are two in the case where microscope carrier lifting driving structure 13, microscope carrier lifting driving structure 13 can be separately positioned on lower layer's microscope carrier fortune On position in defeated platform 112 below the first working region A and third working region C, lower layer's microscope carrier shipping platform as a result, The microscope carrier 2 of the initial storage area D of 112 microscope carrier can be moved to by the lift cylinders 132 that a microscope carrier goes up and down driving structure 13 On upper layer microscope carrier shipping platform 111, and the microscope carrier 2 that detection nucleic acid is completed in the C of third working region can pass through other load The lift cylinders 132 of platform lifting driving structure 13 are moved on lower layer's microscope carrier shipping platform 112, at this point, the transport of lower layer's microscope carrier is flat The structure of microscope carrier driven in translation structure 12 as described above etc. can be set on platform 112, to be moved into down for driving Microscope carrier 2 on layer microscope carrier shipping platform 112 is moved to the initial storage area D of microscope carrier.
For another example, in the case where microscope carrier lifting driving structure 13 is provided with one, optionally, as shown in figure 11, microscope carrier fortune Microscope carrier return driving structure 18 is provided on 1 side wall of transfer mechanism, the microscope carrier return driving structure 18 and lifting fixed pedestal 131 connect It connects, and for driving direction of the microscope carrier lifting driving structure 13 along microscope carrier moving direction Z1 or in contrast to microscope carrier moving direction Z1 to move It is dynamic.The cooperation of driving structure 13 need to be only gone up and down by microscope carrier return driving structure 18 and a microscope carrier as a result, that is, microscope carrier return Driving structure 18 drives the microscope carrier lifting driving structure 13 for being mounted with microscope carrier 2 mobile, so that microscope carrier 2 is transported in lower layer's microscope carrier Position is adjusted on defeated platform 112 to the initial storage area D of microscope carrier so that microscope carrier transport establishment overall structure it is simpler and Rationalize.Here, here, microscope carrier return driving structure 18 can use the arrangement of various reasonable, for example, microscope carrier return drives Dynamic structure 18 can be for can be along structures such as the microscope carrier moving direction Z1 telescoping cylinders to stretch, alternatively, microscope carrier return structure 18 can be with For feed screw nut fit structure etc., in the case, nut is fixed on the lifting fixed pedestal 131 of microscope carrier lifting driving structure 13 On, screw rod can extend to the other end from one end of lower layer's microscope carrier shipping platform 112, and thus, it is possible to pass through nut on lead screw It slides to drive the whole direction along microscope carrier moving direction Z1 or in contrast to microscope carrier moving direction Z1 of microscope carrier lifting driving structure 13 It is mobile.
But the microscope carrier return driving structure 18 of the disclosure is not limited to the above embodiment, for example, optionally, microscope carrier returns Position driving structure 18 includes the second driving motor 181 and the second biography being connected with the transmission of the output shaft of second driving motor 181 Dynamic component, lifting fixed pedestal 131 are mounted on the movable part of the second transmission component, and the second transmission component is used for the second driving The rotary motion of motor 181 is converted to microscope carrier lifting driving structure 13 along the linear movement of microscope carrier moving direction Z1, or on the contrary Linear movement in the direction of microscope carrier moving direction Z1.So that microscope carrier lifting driving structure 13 drives microscope carrier 2 to carry from upper layer It is detached from and is moved on lower layer's microscope carrier shipping platform 112 on platform shipping platform 111, and will be carried by microscope carrier return driving structure 18 Platform 2 is moved to the initial storage area D of microscope carrier, or is detached from from lower layer's microscope carrier shipping platform 112 and is moved to upper layer microscope carrier fortune The initial storage area D of the microscope carrier of defeated platform 111.Here, the second transmission component can use the structure of various reasonable, such as optional Ground, the second transmission component be V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission matching mechanism, thus, it is possible to Enough improve reliable transmission and transmission efficiency.By structure as described above, that is, start the second driving motor 181 its is defeated The rotary force of force-output shaft is converted to the linear movement that microscope carrier goes up and down driving structure 13 by the transmitting of the first transmission component, guarantees Microscope carrier goes up and down reliable mobile of the driving structure 13 on lower layer's microscope carrier shipping platform 112, and then ensure that microscope carrier 2 in lower layer's microscope carrier The transportation of shipping platform 112.
Optionally, the second transmission component can be formed as and the first transmission group in microscope carrier driven in translation structure as described above The identical structure of part, specifically optionally, as shown in Figure 25 and Figure 27, the second transmission component is V belt translation matching mechanism, and including With lifting fixed pedestal 131 connect the second transmission belt 182, be arranged 182 side of the second transmission belt and with the second driving motor Second driving wheel 183 of 181 output axis connection, the second driven wheel 184 that 182 other side of the second transmission belt is set, lifting Fixed pedestal 131 is fixed on the second transmission belt 182, wherein V belt translation matching mechanism has stable drive, buffering absorbing, knot The advantages that structure is simple, at low cost, working service facilitates only passes through simple knot as described above on lower layer's microscope carrier shipping platform 112 The V belt translation matching mechanism of structure allows for and the second transmission belt 182 is reliable and easily drive microscope carrier goes up and down driving structure 13 It is realized in moving range flexibly mobile.
Here, optionally, the second transmission component is set in order to which more smoothly and reliably supporting platform goes up and down driving structure 13 It is equipped with two groups and is separately positioned on the two sides inner wall of lower layer's microscope carrier shipping platform 112, two groups of the second driven wheel 184 passes through biography Moving axis 185 mutually interlocks, wherein in order to rise during the second transmission belt 182 drives microscope carrier lifting driving structure 13 mobile It is remained to guiding microscope carrier lifting driving structure 13 and does linear movement, optionally, as shown in figure 9, lower layer's microscope carrier shipping platform It is located on the position of 182 lower section of the second transmission belt in 112 two sides inner wall, is respectively arranged with and extends along microscope carrier moving direction Z1 And the lifting fixed pedestal guide rail 133 with the two sidewalls cooperation of lifting fixed pedestal 131.Wherein, by structure as described above Microscope carrier goes up and down driving structure 13 suitable for driving microscope carrier 2 rising so that going up and down fixed pedestal 131 in the case where sample processing device It moves on drop fixed pedestal guide rail 133 and microscope carrier 2 is allow to return back to the initial storage area D of microscope carrier.Microscope carrier goes up and down driving structure First arranged on microscope carrier bottom in microscope carrier guide frame as described above can be set in the bottom of 13 lifting fixed pedestal 131 The mutually isostructural third pulley assembly of second pulley component 127 of pulley assembly 23 and the bottom arrangement of movable trolley 122.Microscope carrier Lifting driving structure 13 is rollably supported on lifting fixed pedestal guide rail 133 by third pulley assembly, so that lifting is solid Determine base rail 133 can be smoothly directing to microscope carrier lifting driving structure 13 moved along microscope carrier moving direction Z1 or in contrast to microscope carrier The linear movement in the direction of direction Z1, simultaneously, additionally it is possible to pass through the rolling of third pulley assembly and lifting fixed pedestal guide rail 133 Move cooperation and movement that is more flexible and easily realizing microscope carrier lifting driving structure 13.
In addition, in the sample processing device of the disclosure, optionally, as in figs. 3 and 11, the initial storage area D of microscope carrier It is arranged in the side of upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 and is located at close to the first workspace The region of domain A, microscope carrier transport establishment 1 corresponding to the initial storage area D of microscope carrier side wall on be formed be used for so that microscope carrier 2 stretch out Or the microscope carrier retracted picks and places opening 14, microscope carrier 2 is set as, and picking and placing opening 14 by microscope carrier in the initial storage area D of microscope carrier can It stretches out or retracts relative to microscope carrier transport establishment 1.Here, microscope carrier 2 is configured to fixing seat 24 as described above and scalable The structure of the sliding seat 25 in fixing seat 24 is arranged in ground, as a result, in the case where microscope carrier 2 is located at the initial storage area D of microscope carrier, When needing to pick and place the sample container 3 on microscope carrier 2,24 are done relative to regulation by sliding seat 25 and is stretched along the first telescopic direction P Contracting and opening 14 can be picked and placed from microscope carrier expose or be hidden into microscope carrier and pick and place in opening 14, thus improve sample container 3 and take Efficiency and speed are put, that improves sample processing device recycles efficiency.
Optionally, as shown in Figures 4 to 7, working region further includes first of the sample dispensing for the first working region A Consumptive material storage area E, first mechanical arm 6 are arranged in the first consumptive material storage area E, and first consumptive material storage area E, which has, to be used In the sampling hammerhead storage area E1 of storage sampling hammerhead 200 and for storing the sampling for accommodating the probe tube 300 of sample Pipe storage area E2, the first consumptive material storage area E are arranged on upper layer microscope carrier shipping platform 111 and are located at the initial storage area of microscope carrier The top of domain D, first mechanical arm 6, which is arranged, in the first consumptive material storage area E and to be arranged as, can in the first working region A and It moves back and forth between first consumptive material storage area E, is accommodated with the sample that can be mounted in on the first working region A on microscope carrier 2 Sample in device 3 in dispensing probe tube 300.As a result, each working region arrangement in microscope carrier transport establishment 1 is more reasonable Change, by the way that the first consumptive material storage area E to be disposed adjacent to the top position of the first working region A, the loading of first mechanical arm 6 is taken After sampling hammerhead 200 in sample pipette tips storage area E1, it is moved in the E2 of probe tube storage area and draws in probe tube 300 Sample, be moved to the first working region A later and on microscope carrier injecting sample in each sample container 3, thus more The operation of dispensing sample is quickly and easily executed, and sample process easy to automate operates.
Optionally, as shown in fig. 7, working region further includes the dispensing detection reagent and extraction for third working region C Nucleic acid the second consumptive material storage area F, second mechanical arm 7 be arranged in the second consumptive material storage area F, second consumptive material storage Region F has the dispensing pipette tips storage area F1 for being used to store dispensing pipette tips 400, deposits for storing the deep-well plates of deep-well plates 100 Put region F2 and for storing the detection reagent receiving pipe storage area for accommodating the detection reagent receiving pipe 500 of detection reagent F3, the second consumptive material storage area F are arranged in the other side of upper layer microscope carrier shipping platform 111 and are located at close to third working region C Region, second mechanical arm 7 can move back and forth between third working region C and the second consumptive material storage area F, with can The detection reagent in detection reagent receiving pipe 500 is dispensed in multiple deep-well plates 100 into the second consumptive material storage area F respectively With the nucleic acid being mounted in the C of third working region in the sample container 3 on microscope carrier 2, to detect nucleic acid.Lead to as a result, The position that the second consumptive material storage area F is disposed adjacent to third working region C is crossed, second mechanical arm 7 is moved to dispensing pipette tips Storage area F1 and load dispensing pipette tips 400 after, be moved to detection reagent accommodate pipe storage area F3 and draw detection reagent The detection reagent in pipe 500 is accommodated, deep-well plates storage area F2 is moved to later and injects detection reagent into deep-well plates 100, After second mechanical arm 7 continues the dispensing pipette tips 400 for reloading new later, moves third working region C and draw each on microscope carrier Nucleic acid in a sample container 3, is moved to deep-well plates storage area F2 later and injects nucleic acid into deep-well plates 100, thus More quickly and easily to execute the operation of dispensing detection reagent and nucleic acid, and sample process easy to automate behaviour Make.
To sum up, each structure, that is, nucleic acid extraction mechanism of sample processing device, pipette tips ejection releasing mechanism, microscope carrier are transported The structures such as mechanism and microscope carrier and the cooperation assembly of the two, microscope carrier location structure, microscope carrier guiding mechanism are described in detail.Herein It should be noted that the relevant technologies feature etc. in each structure can be combined in any combination, it is this to combine To technical solution obviously also belong to the protection scope of the disclosure.For example, microscope carrier positioning knot can be set on the same microscope carrier 2 The drawer that the first pulley component 23 in the first positioning region, microscope carrier guide frame, fixing seat 24 and sliding seat 25 in structure cooperate Various features in formula construction, for another example, being provided with as described above can in the microscope carrier transport establishment 1 of multilayer microscope carrier shipping platform 11 Be provided in microscope carrier location structure as described above the second positioning region, the microscope carrier guide rail 17 in microscope carrier guide frame, microscope carrier Driven in translation structure 12, microscope carrier lifting driving structure 13, microscope carrier pick and place various features in opening 14 etc..Those skilled in the art It is to be understood that combination as described above is fallen in the protection scope of the disclosure.
Based on the structure of sample processing device as described above, below to the sample process using above-mentioned sample processing device Method is illustrated.Here, the sample processing method in order to more fully, more clearly illustrate the disclosure, following middle samples The sample processing device that processing method is related to include all the relevant technologies features as described above preferred embodiment Construction is to be described in detail.But this only makes as more comprehensively, clearly explaining the technical solution of the disclosure , it is not used as the purposes of restriction but disclosure protection scope.
Referring to figs. 1 to Figure 44, the disclosure provides a kind of following sample process using sample processing device as described above Method.The sample processing method includes: first step S1, and microscope carrier 2 is moved to the first working region along microscope carrier moving direction Z1 A, using dispensing sample in first mechanical arm 6 respectively each sample container 3 on microscope carrier 2；Second step S2, microscope carrier 2 from First working region A is moved to the second working region B along microscope carrier moving direction Z1, is extracted on microscope carrier 2 using nucleic acid extraction mechanism 4 Sample container 3 in sample contained in nucleic acid, and the nucleic acid of extraction is stored to each sample container 3；Third step Rapid S3, microscope carrier 2 are moved to third working region C from the second working region B, have detection reagent to dispensing using second mechanical arm 7 Multiple deep-well plates 100 in dispensing microscope carrier 2 on sample container 3 in store nucleic acid and detect nucleic acid, from first work On the microscope carrier moving direction Z1 of region A to third working region C, the microscope carrier 2 in adjacent every two microscope carrier 2 positioned at front side is being held While any one step into third step S3 of row first step S1, any one step is executed positioned at the microscope carrier 2 of rear side Rapid previous step.
Here, executing sample processing method as described above using the sample processing device of structure as described above.Such as Motion mode of the microscope carrier 2 in microscope carrier transport establishment 1 in the upper sample processing device can be using the master voluntarily controlled Dynamic driving method, can also be using the passive matrix mode passively controlled by other structures, as long as enabling to microscope carrier 2 mobile Corresponding work is executed to each working region.Sample processing method above disclosured is used in microscope carrier Transport establishment 1 moves up the movable sample process mode of microscope carrier of dynamic load platform 2, and not uses in existing and usually pass through moving machine The mode of tool arm executes the fixed sample process mode of microscope carrier of respective sample processing operation to microscope carrier.Therefore, the disclosure is adopted With the sample under the sample processing method and the existing middle stationary state using microscope carrier of movable microscope carrier by mobile machine arm mode Processing method is compared, and multiple microscope carriers 2 of the disclosure can be performed simultaneously corresponding operation in corresponding working region.Specifically Ground, for example, being located at first being located on the foremost side first microscope carrier 2 on microscope carrier moving direction Z1 when executing third step S3 Second microscope carrier 2 of the next side of a microscope carrier 2 executes second step S2, meanwhile, the third positioned at second next side of microscope carrier 2 carries Platform 2 executes first step S1, synchronously executes in corresponding working region in multiple microscope carriers 2 of same period as a result, Respective operation so as to the activity duration of processing needed for significantly shortening entire sample, and then significantly improves at sample Manage efficiency.
Optionally, as described in Figure 7, working region further includes the second consumptive material storage area F, second consumptive material storage area F With the dispensing pipette tips storage area F1 for storing dispensing pipette tips 400, the deep-well plates storage area for storing deep-well plates 100 F2 and for store accommodate detection reagent detection reagent accommodate pipe 500 detection reagent accommodate pipe storage area F3, second Consumptive material storage area F is arranged in the region of the close third working region C of microscope carrier transport establishment 1, and the setting of second mechanical arm 7 is the It in two consumptive material storage area F and is arranged as, can be moved back and forth between third working region C and the second consumptive material storage area F, The detection in detection reagent container 500 can be dispensed respectively in multiple deep-well plates 100 into the second consumptive material storage area F Reagent and, the nucleic acid being mounted in the C of third working region in the sample container 3 on microscope carrier 2, in third step S3, Before second mechanical arm 7 dispenses the nucleic acid dispensing step S32 of nucleic acid into the deep-well plates 100 of the second consumptive material storage area F, It further include detection reagent dispensing step S31, using second mechanical arm 7 into multiple deep-well plates 100 of the second consumptive material storage area F Detection reagent is dispensed, while starting to execute first step S1, the synchronous detection reagent that executes dispenses step S31.That is, making Detection reagent dispensing step S31 in one step S1 and third step S3 is synchronously executed, thus on microscope carrier moving direction Z1 It is located on the foremost side the detection in third step S3 when first microscope carrier 2 be sequentially completed first step S1 and second step S2 Reagent dispensing step S31 also terminates or terminates in advance just, so that first microscope carrier 2, which eliminates, waits detection reagent point The nucleic acid that note step S31 completes the required time and can directly execute third step S3 dispenses operation, to save considerably The time required to sample process, sample process efficiency is further improved.Specifically, in the detection reagent point for executing third step S3 Infuse step S31 when, second mechanical arm 7 be moved to dispensing pipette tips storage area F1 and load dispensing pipette tips 400 after, be moved to Detection reagent accommodates pipe storage area F3 and draws the detection reagent in detection reagent receiving pipe 500, is moved to deep-well plates later Storage area F2 and detection reagent is injected into deep-well plates 100, second mechanical arm 7 continues the dispensing rifle for reloading new later After first 400, mobile third working region C and draw the nucleic acid that extraction is completed on microscope carrier in each sample container 3, move back It moves to deep-well plates storage area F2 and injects nucleic acid into deep-well plates 100, thus examined more quickly and easily to execute dispensing The operation of test agent and nucleic acid, and sample process easy to automate operates.
Optionally, in first step S1, the sample in probe tube 300 is drawn after the loading sampling hammerhead 200 of first mechanical arm 6 Originally sample is dispensed in each sample container 3 on microscope carrier 2, the sampling hammerhead 200 that would be used for dispensing sample later is set In in sample container 3.That is, the sampling hammerhead 200 for being used in dispensing sample in first step S1 can be placed into sample appearance It receives in device 3 and can be re-used in second step S2, in other words, the sampling gun for being used to dispense sample in first step S1 Head can share a pipette tips for extracting the sampling hammerhead of nucleic acid in second step S2, therefore save on sampling hammerhead, save Consumptive material resource, and then save sample process cost.
Further optionally, in first step S1, in first mechanical arm 6 in each sample container 3 on microscope carrier 2 It further include internal standard dispensing step S11 before the sample dispensing step S12 for dispensing sample, first mechanical arm 6 loads sampling hammerhead Internal standard is drawn after 200 and dispenses internal standard in each sample container 3 on microscope carrier 2, be would be used for dispensing interior target later and is taken Sample pipette tips 200 are discarded.Specifically, in internal standard dispensing step S11, first mechanical arm 6 draws internal standard after loading sampling hammerhead, And internal standard is dispensed in the cell pyrolysis liquid holding tank of each sample container 3 on the microscope carrier 2 into the first working region A, it Target sampling hammerhead 200 in dispensing will be used in afterwards to discard.Internal standard as described above is using whole noncompetitive internal standard, thus It can monitor the overall process of nucleic acid extraction and augmentation detection, while avoid false negative.Here, accommodating interior target internal standard Accommodating pipe can be individually positioned on the corresponding position of each microscope carrier, such as can be formed on the sliding seat top plate 254 of microscope carrier 2 There is the internal standard holding tank 29 that pipe is accommodated for accommodating internal standard, in order to interior target dispensing.But it's not limited to that for the disclosure, described Internal standard accommodates pipe and can also be disposed adjacent on the position of the first working region, for example, as described above first can be arranged in On the E of consumptive material storage area.
Optionally, as shown in Fig. 5, Fig. 6 and Figure 12, nucleic acid extraction mechanism 4 includes: pipette tips mobile device 41, which moves Dynamic device 41 can be arranged in up and down in microscope carrier transport establishment 1 along height direction H, rifle is separatably arranged in for driving Sampling hammerhead 200 on head moving device 41 enables to sampling hammerhead 200 to adsorb sample along height direction H shift position The liquid in liquid or discharge sampling hammerhead 200 in container 3；Magnetic bead transfer device 42, the magnetic bead transfer device 42 include Magnetic part in microscope carrier transport establishment 1 can be telescopically set along the direction close to or far from sampling hammerhead 200, with can So that magnetic part, which has, acts on sampling hammerhead 200 for magnetic state and removing to the magnetic bead offer magnetic force in sampling hammerhead 200 The demagnetizing state of the magnetic force of interior magnetic bead, microscope carrier driving mechanism includes microscope carrier driven in translation structure 12, for driving microscope carrier 2 right Position is adjusted along microscope carrier moving direction Z1 in the working region for the microscope carrier shipping platform 11 answered, and microscope carrier driven in translation structure 12 is set It is set to, in second step S2, can drive microscope carrier 2 in the second working region B along as in contrast to microscope carrier moving direction Z1's Direction far from third working region C is advanced with step-by-step movement, can pass through the lifting action and magnetic with pipette tips mobile device 41 The cooperation of the expanding-contracting action of pearl transfer device 42 is sequentially inserted into each holding tank of sample container 3 and extracts sample appearance Nucleic acid contained in the sample received in device 3, and the nucleic acid of extraction is stored to each sample container 3.
Containing stage translation driving structure 12 in the second working region B as a result, drives microscope carrier 2 towards in contrast to microscope carrier movement side Step-by-step movement backward movement is carried out to the rear side of Z1, is turned from there through with the pipette tips mobile device 41 and magnetic bead of nucleic acid extraction mechanism 4 The cooperation of moving device 42 stores the nucleic acid of extraction to the nucleic acid holding tank of 3 front side of sample container.It is flat by microscope carrier later It moves driving structure 12 and the microscope carrier 2 for completing nucleic acid extraction is moved to third work along microscope carrier moving direction Z1 from the second working region B Make region C, at this point, being stored in due to the nucleic acid extracted in the nucleic acid holding tank of 3 forward position of sample container, makes It obtains second mechanical arm 7 and quickly and easily dispenses the nucleic acid into multiple deep-well plates 100 by the way of smaller movement routine, Further improve sample process operating efficiency.
In addition, the microscope carrier driven in translation structure 12, the pipette tips mobile device 41 that are used in sample processing method as described above With magnetic bead transfer device 42 can using the microscope carrier driven in translation structure 12 referred in microscope carrier transport establishment as described above and The pipette tips mobile device 41 referred in nucleic acid extraction mechanism 4 construction identical with the structure of magnetic bead transfer device 42.Herein in order to It avoids repeating to omit its description.
Further optionally, as shown in figure 43, second step S2 includes: that sampling hammerhead loads step, pipette tips mobile device 41 Load sampling hammerhead 200；Cleavage step S21, sampling hammerhead 200 draw sample and contain the thin of magnetic bead into sample container 3 The sample is added in cellular lysate liquid, so that obtaining the bead complexes that adsorption has nucleic acid, sampling hammerhead 200 after sample cracking Draw the solution containing bead complexes, magnetic part close to sampling hammerhead direction stretch out for magnetic state under, sampling hammerhead 200 solution to spue in addition to the bead complexes being adsorbed on 200 inner wall of sampling hammerhead；Washing step S22, it is remote in magnetic part Under the demagnetizing state that direction from sampling hammerhead 200 retracts, sampling hammerhead 200 draws the cleaning solution in sample container 3 and anti- Compressing movement is executed again, and to clean bead complexes, later under for magnetic state, sampling hammerhead 200 spues except multiple containing magnetic bead Close the solution of object；Elution step S23, under demagnetizing state, sampling hammerhead 200 draws the eluent and anti-in sample container 3 Compressing movement is executed again, so that magnetic bead and nucleic acid separation in bead complexes；Nucleic acid extraction step S24, for magnetic state Under, sampling hammerhead 200 will be except the nucleic acid containing magnetic bead spues and saves to sample container 3；Sampling hammerhead unloading step, rifle Sampling hammerhead 200 is offloaded to scrap area by head moving device 41.Here, optionally, in cleavage step S21, in sampling hammerhead It further include protein digestibility step S25 after 200 draw the solution containing bead complexes, under demagnetizing state, sampling hammerhead Solution containing bead complexes is spued into the protein digestibility enzyme solutions in sample container 3 and executes suction repeatedly by 200 Movement is spat, with the protein in digestion solution, later under for magnetic state, sampling hammerhead 200, which spues to remove, contains bead complexes Solution, to complete cleavage step S21.Cleavage step S21 to nucleic acid extraction step S24 and institute as above in second step S2 It is identical to nucleic acid extraction step S24 to state the cleavage step S21 referred in method for extracting nucleic acid, herein in order to avoid repetition omission pair Its explanation and possessed function and effect.Although in addition, being adopted in the second step S2 of the sample processing method of the disclosure The step-by-step system retreated with the direction by microscope carrier with edge in contrast to microscope carrier moving direction Z1, so that the nucleic acid extracted is placed in In the most nucleic acid holding tank of front side of sample container 3, in order to which subsequent second mechanical arm 7 executes the operation of dispensing nucleic acid, but It's not limited to that for the disclosure, and microscope carrier driven in translation structure 12 may be set to be, and microscope carrier 2 can be driven in the second working region B is advanced along microscope carrier moving direction Z1 with step-by-step movement, and in the case, cell pyrolysis liquid holding tank, protein digestibility enzyme solutions hold Receive slot, cleaning solution holding tank, eluent holding tank and nucleic acid holding tank on sample container along in contrast to microscope carrier moving direction The direction of Z1 is sequentially arranged, and the nucleic acid extracted is then placed in the nucleic acid holding tank positioned at rear side of sample container.
Optionally, microscope carrier transport establishment 1 includes arranging in at least partly described working region along height direction H perforation Upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112, the first working region A, the second working region B and Three working region C are sequentially arranged on upper layer microscope carrier shipping platform 111 along microscope carrier moving direction Z1, and working region further includes being used for The initial storage area D of microscope carrier of multiple microscope carriers 2 is stored, the initial storage area D of microscope carrier is arranged in upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 side and be located at close to the region of the first working region A, microscope carrier driving mechanism includes Microscope carrier driven in translation structure 12 and microscope carrier go up and down driving structure 13, and the setting of microscope carrier driven in translation structure 12 is flat in the transport of upper layer microscope carrier On platform 111, and for driving microscope carrier 2 to adjust position along microscope carrier moving direction Z1 on upper layer microscope carrier shipping platform 111, so that It obtains microscope carrier 2 to be moved to corresponding working region and successively execute first step S1 to third step S3, microscope carrier goes up and down driving structure 13 are arranged on lower layer's microscope carrier shipping platform 112, and for driving microscope carrier 2 to go up and down along height direction H, so that microscope carrier 2 is upper Switching position between layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112, after third step S3, sample process Method further includes microscope carrier return step S4, is transferred to the microscope carrier 2 of third working region C using microscope carrier lifting driving structure 13 The initial storage area D of microscope carrier.Here, microscope carrier driven in translation structure 12 and microscope carrier lifting driving structure 13 can use and institute as above The microscope carrier driven in translation structure 12 referred in the microscope carrier transport establishment 1 stated structure identical with the microscope carrier lifting structure of driving structure 13 It makes, by structure as described above and sample processing method, sample processing device can be carried when executing sample process on upper layer Most sample process operation is executed on platform shipping platform 111, i.e., successively executes first on upper layer microscope carrier shipping platform 111 Step S1, second step S2 and third step S3, the microscope carrier 2 after completing third step S3 can be gone up and down by microscope carrier to be driven Structure 13 is transferred to the initial position of the initial storage area D of microscope carrier, that is, for example, as shown in Figures 2 and 3, microscope carrier 2 is provided with In the case where six, wherein three microscope carriers 2 can be arranged in the initial storage area D of microscope carrier of upper layer microscope carrier shipping platform 111, Its excess-three microscope carrier 2 can be arranged in the initial storage area D of microscope carrier of lower layer's microscope carrier shipping platform 112.In the case, it refers to Fig. 5 and Fig. 6, if first microscope carrier 2 for being located at the most front side of upper layer microscope carrier shipping platform 111 is sequentially completed first step S1 extremely After third step S3, driving structure 13 is gone up and down for first microscope carrier 2 from the third of upper layer microscope carrier shipping platform 11 by microscope carrier Working region C is via the initial storage area D of microscope carrier that lower layer's microscope carrier shipping platform 112 is transferred to upper layer microscope carrier shipping platform 111 Initial position, that is, each microscope carrier 2 completes entire sample process using closed-loop shaped movement routine along microscope carrier moving direction Z1 The initial position of the initial storage area D of microscope carrier is returned to after operation, consequently facilitating the sample process of subsequent cycle.Here, for The position disclosure of the initial storage area D of microscope carrier is not limited to the above embodiment, for example, the initial storage area of microscope carrier D can be all arranged on lower layer's microscope carrier shipping platform 112.Thus it is mainly used to arrange on lower layer's microscope carrier shipping platform 112 The initial storage area D of microscope carrier for storing microscope carrier 2, so that upper layer microscope carrier shipping platform 111 He of the microscope carrier 2 in three-dimensional arrangement Respectively divide the work on lower layer's microscope carrier shipping platform 112 and clearly execute corresponding operation, and then effectively improves sample process operating efficiency.
In addition, as described in above-mentioned sample processing device, optionally, as shown in fig. 7, working region further includes for the The first consumptive material storage area E that sample dispenses in one step S1, first mechanical arm 6 are arranged in the first consumptive material storage area E, First consumptive material storage area E is accommodated with the sampling hammerhead storage area E1 for storing sampling hammerhead 200 and for storing There are the probe tube storage area E2 of the probe tube 300 of sample, the first consumptive material storage area E to be arranged in upper layer microscope carrier shipping platform Top on 111 and positioned at the initial storage area D of microscope carrier, first mechanical arm 6 is arranged in the first consumptive material storage area E and cloth It is set to, can be moved back and forth between the first working region A and the first consumptive material storage area E, with can be to the first working region A On be mounted in the sample container 3 on microscope carrier 2 Nei dispense probe tube 300 in sample.As a result, in microscope carrier transport establishment 1 Each working region arrangement more rationalize, by the way that the first consumptive material storage area E is disposed adjacent to the first working region A's Top position, make in first step S1 first mechanical arm 6 load sampling hammerhead storage area E1 in sampling hammerhead 200 it Afterwards, be moved in the E2 of probe tube storage area and draw the sample in probe tube 300, be moved to the first working region A later and Thus the injecting sample in each sample container 3 on microscope carrier more quickly and easily executes first step S1, and is easy to Realize automation sample process operation.
To sum up, sample processing method is described in detail, here, in order to more comprehensively, clearly demonstrate sample Each step of processing, enumerates specific example herein to illustrate all processes of the sample process of the disclosure, but this is not used as limiting Determine the scope of the present disclosure, but is used only for explaining the sample processing method of the disclosure.Here, in applicable sample processing device All the relevant technologies features as described above are provided with, i.e., include being provided with upper layer load as described above in the sample processing device The microscope carrier transport establishment 1 of platform shipping platform 111 and lower layer's microscope carrier shipping platform 112, microscope carrier guide frame, microscope carrier location structure, It is provided with the microscope carrier 2 of fixing seat 24 and sliding seat 25 and with the pipette tips mobile device for being provided with pipette tips ejection releasing mechanism 5 41 and magnetic bead transfer device 42 nucleic acid extraction mechanism 4.In the sample processing device, set on upper layer microscope carrier shipping platform 111 It is equipped with partial region, the first working region A, the second working region B, third working region C, of the initial storage area D of microscope carrier One consumptive material storage area E and the second consumptive material storage area F, the partial region of the initial storage area D of microscope carrier are positioned close to The position of one working region A is provided with another part region of the initial storage area D of microscope carrier on lower layer's microscope carrier shipping platform 112 And the underface in a part of region of the initial storage area D of microscope carrier on upper layer microscope carrier shipping platform 111, the first consumption Material storage area E is located at the top of the initial storage area D of microscope carrier, the second consumptive material storage area on upper layer microscope carrier shipping platform 111 Domain F is arranged in another side position of the close third working region C of upper layer microscope carrier shipping platform 111, and on upper layer, microscope carrier transport is flat It is provided with microscope carrier driven in translation structure 12 on platform 111, microscope carrier lifting driving structure is provided on lower layer's microscope carrier shipping platform 112 13 and microscope carrier return driving structure 18.In addition, there are six microscope carriers 2 for setting altogether in above-mentioned sample processing device, wherein three loads Platform 2 can be arranged in the initial storage area D of microscope carrier of upper layer microscope carrier shipping platform 111, and excess-three microscope carrier 2 can be arranged in down The initial storage area D of microscope carrier of layer microscope carrier shipping platform 112.Here, on microscope carrier moving direction Z1 from be located at upper layer microscope carrier The microscope carrier of the most front side (i.e. the top side for being located at upper layer microscope carrier shipping platform 111 in Fig. 5) of shipping platform 111 is carried to lower layer is located at The microscope carrier of the most rear side (i.e. the top side for being located at lower layer's microscope carrier shipping platform 112 in Fig. 6) of platform shipping platform 111 is successively defined as First microscope carrier to the 6th microscope carrier.In addition, can be set on each microscope carrier, there are four sample containers 3, and each sample container 3 Four groups of holding tank groups for extracting four nucleic acid inside can be set, thus sample processing device as described above is being run When can disposably execute the processing of 96 samples, therefore use the deep-well plates 100 in 96 holes accordingly to detect 96 nucleic acid.
It is specially as follows to its sample process process with reference to Figure 43 and Figure 44 based on sample processing device as described above.
Before running sample processing device, as shown in Figures 2 and 3, each microscope carrier 2 can be by sliding seat 25 from microscope carrier Transport establishment 1 corresponds to the microscope carrier formed on the initial storage area D of microscope carrier and picks and places 14 stretching of opening, respectively into each microscope carrier 2 It is accordingly put into sample container 3, has wherein been put into cell pyrolysis liquid in each holding tank of sample container 3, protein disappears Change enzyme solutions, cleaning solution and eluent and the opening that each sample holding tank is closed by sealing materials such as aluminium foils, here, can After internal standard receiving pipe to be placed on each microscope carrier 2 (such as sliding seat 25) as needed, sliding seat 25 is retracted into original Position, runs sample processing device later.
Firstly, when operation sample processing device, as shown in fig. 7, the internal standard dispensing step S11 and third step of first step S1 Detection reagent dispensing step S31 in rapid S3 is synchronously executed.Pass through first mechanical arm 6 to the first of the first working region A In microscope carrier while dispensing internal standard, detection examination is dispensed into the deep-well plates 100 of deep-well plates storage area F2 by second mechanical arm 7 Agent, specifically, second mechanical arm 7 be moved to dispensing pipette tips storage area F1 and load dispensing pipette tips 400 after, be moved to detection Reagent accommodates pipe storage area F3 and draws the detection reagent in detection reagent receiving pipe 500, is moved to deep-well plates storage later Region F2 and inject detection reagent into deep-well plates 100.
In first step S1, the first driving motor 121 of starting microscope carrier driven in translation structure 12 operates and drives first First transmission belt 123 of transmission component rotates, and drive activity trolley 122 is moved on trolley guide rail 126 corresponding to first The position of microscope carrier is powered off by the second magnet plunger 81 on movable trolley 122 so that the second magnet plunger 81 pushes away in this case Bar is stretched out and is snapped onto the locking structure 21 of the first microscope carrier, thus the first microscope carrier of locking and movable trolley 122.Later by the The rotation of one transmission belt 123 and drive activity trolley 122 and the first microscope carrier are moved to the first working region A together, upper layer at this time On second positioning seat 15 of microscope carrier shipping platform 111, the trough type photoelectric sensor 16 corresponding to the first working region A detects When the position of the block 222 on the first positioning seat 22 of one microscope carrier, according to the signal that trough type photoelectric sensor 16 detects, upper layer It is located at the first electricity with 16 corresponding position of groove profile photoelectrical position sensor on first working region A of microscope carrier shipping platform 111 Magnetic bar 151 stretches out and cooperates with the locating slot 221 on the first microscope carrier, so that the first microscope carrier is navigated to upper layer microscope carrier shipping platform 111 the first working region A executes first step S1.The internal standard dispensing step S11 of first step S1 is specifically first carried out, the After one mechanical arm 6 loads the sampling hammerhead 200 in the E1 of sampling hammerhead storage area, draw internal standard accommodate internal standard in pipe and to the Internal standard is dispensed in each sample container 3 on the first microscope carrier of one working region A, would be used for dispensing interior target later and take Sample pipette tips 200 are discarded.Later, after the sampling hammerhead 200 that first mechanical arm 6 is reloaded in the E1 of sampling hammerhead storage area, Be moved in the E2 of probe tube storage area and draw the sample in probe tube 300, be moved to the first working region A later and to the Injecting sample in each sample container 3, can would be used for the unloading of sampling hammerhead 200 of sample injection later on one microscope carrier To the sampling gun holding tank of each sample container 3.Here, in order to improve nucleic acid extraction efficiency, in first step S1, Internal standard and sample can be directly injected into the cell cracking liquid bath of sample container 3, that is, accommodated the cell containing magnetic bead and split In the slot for solving liquid, this part steps had both belonged to the sample injection step of first step S1, also belonged to splitting in method for extracting nucleic acid Solve the first half step in step S21, in this case, it is possible to save in cleavage step S21 sampling hammerhead 200 draw sample and to The step of sample is added in the cell pyrolysis liquid containing magnetic bead in sample container 3.After completing first step S1, upper layer is carried Platform shipping platform 111 is powered corresponding to the first magnet plunger 151 of the first working region A, passes through what is generated in the first magnet plunger 151 Magnetic force makes the pusher retracted and is detached from the locating slot 221 of the first microscope carrier, and the first microscope carrier is enabled to follow movable trolley 122 It is mobile.Later, the 123 drive activity trolley 122 of the first transmission belt and the first microscope carrier of microscope carrier driven in translation structure 12 move together To the second working region B, at this time on the second positioning seat 15 of upper layer microscope carrier shipping platform 111, corresponding to the second working region B's When trough type photoelectric sensor 16 detects the position of the block 222 on the first positioning seat 22 of the first microscope carrier, according to groove profile photoelectricity The signal that sensor 16 detects, is located on the second working region B of upper layer microscope carrier shipping platform 111 and the groove profile optoelectronic position First magnet plunger 151 of 16 corresponding position of sensor stretches out and cooperates with the locating slot 221 on the first microscope carrier, thus by first Microscope carrier navigates to the second working region B of upper layer microscope carrier shipping platform 111.The movable trolley 122 for being detached from the first microscope carrier is moved to The position corresponding to the second microscope carrier of upper layer microscope carrier shipping platform 111, and it is latter with being moved to the first work with the second microscope carrier locking Make region A, and the second microscope carrier is navigated into the first working region A, so that the second microscope carrier executes first step S1, carries herein Movable trolley 122 is detached from the second microscope carrier and is moved to the position corresponding to the first microscope carrier of the second working region B under platform, and with the One microscope carrier locking.In the case, upper layer microscope carrier shipping platform 111 corresponds to the first magnet plunger 151 of the second working region B It is powered, the locating slot 221 for making pusher retracted be detached from the first microscope carrier by the magnetic force generated in the first magnet plunger 151, so that First microscope carrier can follow movable trolley 122 mobile, and thus the first microscope carrier executes second step S2.The of second working region B One microscope carrier follows movable trolley 122 movable together in the state of cooperating with movable trolley 122, specifically, in second step S2, First microscope carrier follows movable trolley 122 (to lean on along the direction of the separate third working region C in contrast to microscope carrier moving direction Z1 The direction of nearly first working region A) it is advanced with step-by-step movement, pass through the liter with pipette tips mobile device 41 in step-by-step movement traveling process The cooperation of the expanding-contracting action of drop movement and magnetic bead transfer device 42, the cell pyrolysis liquid for being sequentially inserted into sample container 3 accommodate It executes and extracts in each sample in slot, protein digestibility enzyme solutions holding tank, cleaning solution holding tank and eluent holding tank Contained nucleic acid, and nucleic acid is stored into being located on the foremost side in nucleic acid holding tank to sample container 3.
Later, the 123 drive activity trolley 122 of the first transmission belt and the first microscope carrier of microscope carrier driven in translation structure 12 move together It moves to third working region C.On second positioning seat 15 of upper layer microscope carrier shipping platform 111, the slot corresponding to third working region C When type photoelectric sensor 16 detects the position of block 222 of the first microscope carrier, the letter that is detected according to trough type photoelectric sensor 16 Number, it is located at and 16 corresponding position of groove profile photoelectrical position sensor on the third working region C of upper layer microscope carrier shipping platform 111 The first magnet plunger 151 stretch out and on the first microscope carrier locating slot 221 cooperate, so that the first microscope carrier is navigated to upper layer microscope carrier The third working region C of shipping platform 111 passes through the second magnet plunger at this point, the second magnet plunger 81 of movable trolley 122 is powered The magnetic force generated in 81 make the pusher retracted of the second magnet plunger 81 and from the locking structure 21 for being detached from the first microscope carrier, to unlock First microscope carrier and movable trolley 122.Here, the movable trolley 122 for being detached from the first microscope carrier is moved to upper layer microscope carrier shipping platform 111 the first working region A corresponds to the position of the second microscope carrier, and latter with the second microscope carrier locking of completion first step S1 With being successively moved to the second working region B, and after the second microscope carrier is navigated to the second working region B, movable trolley is detached from second Microscope carrier and the position corresponding to third microscope carrier for being moved to upper layer microscope carrier shipping platform 111, and it is latter same with third microscope carrier locking It is moved to the first working region A, third microscope carrier navigates to the first working region A, so that third microscope carrier executes first step S1.? Under this state, movable trolley 122 is detached from third microscope carrier and is moved to the position corresponding to the second microscope carrier of the second working region B, And pass through the elastic recovery of 151 inner spring of the first magnet plunger of the second working region B in this case with the second microscope carrier locking Power makes pusher retracted and is detached from the locating slot 221 of the second microscope carrier, and the second microscope carrier is enabled to follow movable trolley 122 mobile, Thus the second microscope carrier executes second step S2.That is, at this point, third microscope carrier, the second microscope carrier and the first microscope carrier are corresponding Synchronously executed in one working region A, the second working region B and third working region C first step S1, second step S2 with And third step S3, to significantly improve the operating efficiency of entire sample process.
In addition, executing the nucleic acid dispensing operation of third step S3 positioned at the first microscope carrier of third working region C.First microscope carrier When the nucleic acid for executing third step S3 dispenses operation, it is preferable that the first microscope carrier executes synchronous the executed when first step S1 The detection reagent dispensing step S31 of three step S3 has been fulfiled ahead of schedule or has by chance been completed, and this saves the first microscope carrier in third Time needed for waiting detection reagent dispensing step S31 to complete in the C of working region.In third step S3, second mechanical arm 7 is moved It moves to dispensing pipette tips storage area F1 and after loading dispensing pipette tips 400, moves third working region C and draw the first microscope carrier Nucleic acid in each 3 amplifying nucleic acid holding tank of sample container is moved to deep-well plates storage area F2 and into deep-well plates 100 later Inject nucleic acid.
The first microscope carrier of third working region C executes microscope carrier return after completing the nucleic acid dispensing operation of third step S3 Step S4, that is, driving structure 13 is gone up and down by microscope carrier and microscope carrier return driving structure 18 is moved to lower layer's microscope carrier shipping platform 112 Corresponding position on, for example, can be moved to lower layer's microscope carrier shipping platform 112 close to the 6th microscope carrier position on, Zhi Hou One microscope carrier is finally transferred to the initial position of the initial storage area D of microscope carrier of upper layer microscope carrier shipping platform 111.
Similarly with the above-mentioned course of work, the 4th microscope carrier on lower layer's microscope carrier shipping platform 112, the 5th microscope carrier and the 6th carry Platform is moved into upper layer microscope carrier shipping platform by the cooperation that microscope carrier goes up and down driving structure 13 and microscope carrier return driving structure 18 On 111, and the first working region A, the second work are successively moved to by the movable trolley 122 of microscope carrier driven in translation structure 12 Make region B and third working region C successively executes first step S1, second step S2 and third step S3, later finally Lower layer's microscope carrier shipping platform 112 is moved to by the cooperation that microscope carrier goes up and down driving structure 13 and microscope carrier return driving structure 18 Thus the initial storage area D of microscope carrier completes the processing of 96 samples on six microscope carriers.
To sum up, by sample processing device as described above and sample processing method, multiple microscope carriers be can be realized respective Accordingly step is synchronously executed respectively in corresponding working region, also, when bringing into operation sample processing device, the first step Rapid S1 and detection reagent dispensing step S31 may be performed simultaneously, and thus, it is possible to significantly improve the sample process of entire cycle period Efficiency realizes full-automatic streamlined operation.In addition, since the microscope carrier transport establishment 1 in sample processing device is using multilayer load The three-dimensional arrangement architecture of platform shipping platform 11, therefore area needed for the arrangement of sample processing device can be effectively reduced, it saves Arrangement space.
The preferred embodiment of the disclosure is described in detail in conjunction with attached drawing above, still, the disclosure is not limited to above-mentioned reality The detail in mode is applied, in the range of the technology design of the disclosure, a variety of letters can be carried out to the technical solution of the disclosure Monotropic type, these simple variants belong to the protection scope of the disclosure.
It is further to note that specific technical features described in the above specific embodiments, in not lance In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the disclosure to it is various can No further explanation will be given for the combination of energy.
In addition, any combination can also be carried out between a variety of different embodiments of the disclosure, as long as it is without prejudice to originally Disclosed thought equally should be considered as disclosure disclosure of that.

Claims (37)

1. a kind of sample processing device, which is characterized in that the sample processing device includes microscope carrier transport establishment (1) and movably Multiple microscope carriers (2) on the microscope carrier transport establishment (1) are set, multiple samples are provided on each microscope carrier (2) and are accommodated Device (3), each sample container (3) is interior to accommodate useful multiple reagent solutions in processing sample, the microscope carrier transport establishment (1) On be disposed with multiple working regions, be provided with first mechanical arm (6) and second mechanical arm (7) on the microscope carrier transport establishment (1),
The working region includes at least: the first working region (A), the first mechanical arm (6) are positioned close to described first The position of working region (A), and the first mechanical arm (6) is set as, it can be along the longitudinal direction for being parallel to microscope carrier moving direction (Z1) Direction (X1) and position is adjusted perpendicular to the transverse direction (X2) of the microscope carrier moving direction (Z1), and it can be along short transverse (H) it goes up and down, for dispensing sample to each sample container of the microscope carrier (2) in first working region (A) (3) in；
Second working region (B), second working region (B) is interior to be provided with nucleic acid extraction mechanism (4), the nucleic acid extraction mechanism (4) it is configured to move along the direction close to or far from the microscope carrier (2), for extracting the sample in sample container (3) Nucleic acid contained in this, and the nucleic acid of extraction is stored to each sample container (3)；
Third working region (C), the second mechanical arm (7) are positioned close to the position of the third working region (C), and should Second mechanical arm (7) is set as, and can adjust position along the longitudinal direction (X1) and the transverse direction (X2), and being capable of edge Short transverse (H) lifting, is examined with the nucleic acid for dispensing detection reagent and extraction respectively into multiple deep-well plates (100) Nucleic acid is surveyed,
Each microscope carrier (2) can be along the microscope carrier moving direction (Z1) successively from first work by microscope carrier driving mechanism Make region (A) and move to second working region (B) and the third working region (C), to execute relevant work.
2. sample processing device according to claim 1, which is characterized in that the nucleic acid extraction mechanism (4) includes: pipette tips The microscope carrier transporter can be arranged in up and down in mobile device (41), the pipette tips mobile device (41) along the short transverse (H) On structure (1), for driving the sampling hammerhead (200) being separatably arranged on the pipette tips mobile device (41) along the height The shift position direction (H) is spent, and sampling hammerhead (200) is enabled to adsorb liquid or discharge in the sample container (3) Liquid in the sampling hammerhead (200)；
Magnetic bead transfer device (42), which includes magnetic part, the magnetic part can along close to or far from The direction of sampling hammerhead (200) is telescopically arranged on the microscope carrier transport establishment (1), so that magnetic part has to sampling gun Magnetic bead in head (200) provides going for the magnetic force for the magnetic bead of magnetic force acted in sampling hammerhead (200) for magnetic state and removing Magnetic state.
3. sample processing device according to claim 2, which is characterized in that the pipette tips mobile device (41) includes setting Pedestal (411) on the microscope carrier transport establishment (1) and the pedestal can be set up and down along the short transverse (H) (411) pipette tips on eject releasing mechanism (5), and it includes for installing sampling hammerhead (200) which, which ejects releasing mechanism (5), Sampling gun (51), sampling gun actuator (52) and ejection release piece (53), the driving portion of the sampling gun actuator (52) and institute The telescopic rod (511) for stating sampling gun (51) connects and for driving the telescopic rod (511) flexible, so that the sampling gun (51) suction of imbibition is provided for the sampling hammerhead (200) or the pressure of drain, the ejection release piece (53) are mounted on institute It states on sampling gun actuator (52) and the sampling hammerhead (200) is enabled to separate with the sampling gun (51).
4. sample processing device according to claim 3, which is characterized in that the sampling gun (51) includes main body (512) With multiple telescopic rods (511), fixed pedestal (521) of main body (512) setting in the sampling gun actuator (52) On, and there is multiple telescopic rod channels (513) of the both ends open for the telescopic rod (511) insertion, each telescopic rod (511) top is connect with the driving portion of the sampling gun actuator (52), and each telescopic rod (511) passes through the drive The driving in dynamic portion and can be telescopically inserted into along short transverse (H) in corresponding telescopic rod channel (513), it is described The bottom corresponding to each telescopic rod channel (513) of main body (512) be respectively structured as with the corresponding sampling The pipette tips interconnecting piece (514) of pipette tips (200) connection, the ejection release piece (53) are configured to relative to each pipette tips Interconnecting piece (514) movement, to enable to each sampling hammerhead (200) and corresponding pipette tips interconnecting piece (514) it separates.
5. sample processing device according to claim 4, which is characterized in that the ejection release piece (53) includes being capable of edge The short transverse (H) is supported on the ejection disengaging plate on the fixed pedestal (521) of the sampling gun actuator (52) up and down (531), which is located at the lower section of the main body (512), and the ejection on the short transverse (H) Multiple pipette tips interconnecting piece insertions hole (532) are formed on disengaging plate (531), the pipette tips of each pipette tips interconnecting piece (514) connect Section (515) is connect respectively accordingly to penetrate through pipette tips interconnecting piece insertion hole (532) and be exposed to ejection disengaging plate (531) The driving portion of side, the sampling gun actuator (52) is set as, can under the retracted mode that the telescopic rod (511) retract Ejection disengaging plate (531) is driven to squeeze on each pipette tips interconnecting piece (514) along the off-direction of sampling hammerhead (200) Sampling hammerhead (200).
6. sample processing device according to claim 5, which is characterized in that the ejection release piece (53) includes supporting plate (533) and with described plate (533) and the connecting rod (534) that connect of ejection disengaging plate (531) are supported, it is described to support plate (533) described to support plate (533) and institute between the driving portion and the main body (512) of the sampling gun actuator (52) The fixation can be arranged in up and down along the short transverse (H) by the connecting rod (534) by stating ejection disengaging plate (531) On pedestal (521), the driving portion can abut under the retracted mode with the plate (533) of supporting, to push the top Release piece (53) is moved along the off-direction out, and the connecting rod (534) passes through the reset that is set in the connecting rod (534) Spring (535) is resiliently supported on the fixed pedestal (521), and the both ends of the reset spring (535) are supported with described respectively Plate (533) and the fixed pedestal (521) abut, to drive the ejection release piece (53) to return back to initially always for providing The elastic-restoring force of position.
7. sample processing device according to claim 6, which is characterized in that the sampling gun (51) is in transverse direction (X2) Be positioned apart from it is multiple, it is described to support plate (533) and the ejection disengaging plate (531) is prolonged along the transverse direction (X2) respectively It stretches, the connecting rod (534) is sides that are multiple and being separated with a sampling gun (51) between the transverse direction (X2) respectively Formula setting is supported between plate (533) and the ejection disengaging plate (531) described, so that described support plate (533), the top Sampling gun layout area, Ge Gesuo are respectively constituted between connecting rod (534) described in disengaging plate (531) and adjacent every two out The main body (512) for stating sampling gun (51) is located in the sampling gun layout area.
8. sample processing device according to claim 7, which is characterized in that the sampling gun actuator (52) includes described Fixed pedestal (521), the sampling gun driving motor (522) being arranged on the fixed pedestal (521) drive electricity with the sampling gun The output shaft transmission of machine (522) is connected and is provided with the sampling gun transmission mechanism of the driving portion, and the sampling gun is driven The rotary motion of motor (522) is converted into the linear motion of the driving portion.
9. sample processing device according to claim 8, which is characterized in that the sampling gun transmission mechanism is bolt and nut Transmission mechanism including the output axis connection with the sampling gun driving motor (522) and is pivotally supported at the fixed base Seat (521) on the first screw rod (523) and first screw rod (523) can be movably arranged at along the short transverse (H) On the first nut (524), the driving portion is the driving baffle being set on the outer peripheral surface of first nut (524) (525), the holding tank (526) on the head for clamping the telescopic rod (511) is formed on the driving baffle (525).
10. sample processing device according to claim 2, which is characterized in that the magnetic bead transfer device (42) includes setting Set the magnetic bead transfer device fixing seat (421) on the position in microscope carrier transport establishment (1) close to sampling hammerhead (200), setting exists Magnetic part actuator and magnetic part movable plate (422) on the magnetic bead transfer device fixing seat (421), the magnetic part move Movable plate (422) is arranged on the magnetic bead transfer device fixing seat (421), and connect with the magnetic part actuator with being capable of edge Flexible close to or far from the direction of the sampling hammerhead (200), the magnetic part is magnet (423) and is mounted on the magnetic part Side of the movable plate (422) close to the sampling gun (51).
11. sample processing device according to claim 10, which is characterized in that the magnetic part actuator includes magnetic part Driving motor (426) and magnetic part bolt and nut mechanism, magnetic part driving motor (426) setting is shifted in the magnetic bead to be filled It sets on fixing seat (421), the magnetic part screw rod (427) and the magnetic part driving motor of magnetic part bolt and nut mechanism (426) output axis connection, the magnetic part nut (428) and the magnetic part movable plate of magnetic part bolt and nut mechanism (422) it connects.
12. sample processing device according to claim 11, which is characterized in that shape on the magnetic part movable plate (422) At the long guiding hole (424) for having the telescopic direction extension along the magnetic part movable plate (422), the magnetic bead transfer device (42) On be provided with positioning screw (425) for being inserted into and being installed in the long guiding hole (424), the magnetic part movable plate (422) magnetic bead is telescopically disposed in by the cooperation of the long guiding hole (424) and the positioning screw (425) to shift On device (42).
13. sample processing device according to claim 2, which is characterized in that the microscope carrier transport establishment (1) includes along height Spend direction (H) arrangement multilayer microscope carrier shipping platform (11), first working region (A), second working region (B) and The third working region (C) is arranged in any one in microscope carrier shipping platform (11) described in multilayer, the microscope carrier driving Mechanism includes microscope carrier driven in translation structure (12) and microscope carrier lifting driving structure (13), and the microscope carrier driven in translation structure (12) sets It sets at least one of described microscope carrier shipping platform (11), and for driving the microscope carrier (2) in the corresponding microscope carrier Position is adjusted along microscope carrier moving direction (Z1) in the working region of shipping platform (11), microscope carrier lifting driving structure (13) sets Set in the microscope carrier shipping platform (11) remaining at least one on, and for driving the microscope carrier (2) along short transverse (H) Lifting, so that the microscope carrier (2) switching position between each layer microscope carrier shipping platform (11).
14. sample processing device according to claim 13, which is characterized in that the microscope carrier transport establishment (1) includes edge The upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112) of short transverse (H) perforation ground arrangement, described first Working region (A), second working region (B) and the third working region (C) along microscope carrier moving direction (Z1) successively It is arranged on the upper layer microscope carrier shipping platform (111), the microscope carrier driven in translation structure (12) is arranged in the upper layer microscope carrier On shipping platform (111), microscope carrier lifting driving structure (13) is arranged on lower layer's microscope carrier shipping platform (112), institute Stating working region further includes the initial storage area of microscope carrier (D) for storing multiple microscope carriers (2), and the microscope carrier is initially stored At least part region in region (D) is arranged on lower layer's microscope carrier shipping platform (112).
15. sample processing device according to claim 14, which is characterized in that the microscope carrier driven in translation structure (12) sets It is set to, can drive the microscope carrier (2) in second working region (B) along remote in contrast to the microscope carrier moving direction (Z1) Direction from the third working region (C) is advanced with step-by-step movement, can pass through the pipette tips with the nucleic acid extraction mechanism (4) The cooperation of the expanding-contracting action of the lifting action of mobile device (41) and the magnetic bead transfer device (42) is extracted the sample and is held It receives and nucleic acid contained in the sample in device (3) and stores the nucleic acid of extraction to each sample container (3).
16. sample processing device according to claim 14, which is characterized in that the initial storage area of microscope carrier (D) point It is not arranged in the side of the upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112) and is located at close The region of first working region (A), the microscope carrier transport establishment (1) correspond to the side wall of the initial storage area of microscope carrier (D) On be formed with and be used for so that the microscope carrier that the microscope carrier (2) is stretched out or retracted picks and places opening (14), the microscope carrier (2) is set as, The initial storage area of microscope carrier (D) picks and places opening (14) by the microscope carrier can be relative to the microscope carrier transport establishment (1) It stretches out or retracts.
17. sample processing device according to claim 16, which is characterized in that the working region further includes for described First consumptive material storage area (E) of the sample dispensing of the first working region (A), first mechanical arm (6) setting is in the first consumption In material storage area (E), which there is the sampling hammerhead for storing sampling hammerhead (200) to store Region (E1) and for storing the probe tube storage area (E2) for accommodating the probe tube (300) of sample, first consumptive material is deposited Region (E) is put to be arranged on the upper layer microscope carrier shipping platform (111) and be located at the upper of the initial storage area of the microscope carrier (D) Side, first mechanical arm (6) setting is in first consumptive material storage area (E) and is arranged as, can be in first work Make to move back and forth between region (A) and first consumptive material storage area (E), can carry on the first working region (A) Sample in the sample container (3) on the microscope carrier (2) in dispensing probe tube (300).
18. sample processing device according to claim 14, which is characterized in that the working region further includes for third Second consumptive material storage area (F) of the nucleic acid of the dispensing detection reagent and extraction of working region (C), the second mechanical arm (7) In second consumptive material storage area (F), which has for storing dispensing pipette tips for setting (400) dispensing pipette tips storage area (F1), the deep-well plates storage area (F2) for storing deep-well plates (100) and for depositing Put detection reagent receiving pipe storage area (F3) for accommodating the detection reagent receiving pipe (500) of detection reagent, second consumption Material storage area (F) is arranged the other side in the upper layer microscope carrier shipping platform (111) and is located at close to the third workspace The region in domain (C), the second mechanical arm (7) can be in the third working regions (C) and second consumptive material storage area (F) it is moved back and forth between, can distinguish in multiple deep-well plates (100) into second consumptive material storage area (F) Dispense the detection reagent accommodate detection reagent in pipe (500) and, be located in the third working region (C) be mounted in it is described The nucleic acid in sample container (3) on microscope carrier (2), to detect nucleic acid.
19. sample processing device according to claim 14, which is characterized in that the microscope carrier driven in translation structure (12) sets It sets on the side inner wall of the upper layer microscope carrier shipping platform (111), and including the first driving motor (121) and first drive It the first connected transmission component of the output shaft transmission of dynamic motor (121) and is connect with first transmission component and can be with The movable trolley (122) that the microscope carrier separatably cooperates, first transmission component are used for first driving motor (121) rotary motion is converted to the linear movement of the movable trolley (122), to enable to the movable trolley (122) The working region locating for the microscope carrier (2) is adjusted by the cooperation with the microscope carrier (2).
20. sample processing device according to claim 19, which is characterized in that first transmission component is matched for V belt translation Close mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission matching mechanism.
21. sample processing device according to claim 20, which is characterized in that first transmission component is matched for V belt translation Mechanism is closed, and drives electricity including the first transmission belt (123), setting in the first transmission belt (123) side and with described first The first driving wheel (124) of the output axis connection of machine (121) and setting are the first of the first transmission belt (123) other side Driven wheel (125), the activity trolley (122) are mounted on first transmission belt (123).
22. sample processing device according to claim 21, which is characterized in that the upper layer microscope carrier shipping platform (111) In be located at first transmission belt (123) downside inner wall section on be provided with along the microscope carrier moving direction (Z1) extend platform Vehicle guide rail (126), the activity trolley (122) are fixed in the downside band part of first transmission belt (123), the work The bottom of dynamic trolley (122) is provided with the slideably second pulley component (127) with the trolley guide rail (126) cooperation.
23. sample processing device according to claim 22, which is characterized in that the second pulley component (127) passes through Second pulley mounting base (128) setting is located in the bottom of the movable trolley (122), the second pulley mounting base (128) It is supported on the trolley guide rail (126) on the downside of first transmission belt (123) and by the second pulley component (127).
24. sample processing device according to claim 23, which is characterized in that scalable on the activity trolley (122) Ground is provided with stretching structure (8), and the microscope carrier (2) corresponds on the side side wall of the movable trolley (122), is provided with and is used for The locking structure (21) for cooperating with the stretching structure (8) and being mutually locked.
25. sample processing device according to claim 24, which is characterized in that the stretching structure (8) includes the second electricity Magnetic bar (81), second magnet plunger (81) has lockup state and unlocked state, in the lockup state, second electromagnetism Bar (81) power-off, so that the push rod of the second magnet plunger (81) is stretched out and snapped on the locking structure (21) and described in locking Microscope carrier (2) and the movable trolley (122)；In the unlocked state, second magnet plunger (81) is powered, to pass through described the The magnetic force generated in two magnet plungers (81) makes the pusher retracted and unlocks the microscope carrier (2) and the movable trolley (122), The locking structure (21) is formed to have lock groove or locking hole with the shape of second magnet plunger (81) cooperation.
26. sample processing device according to claim 25, which is characterized in that the stretching structure (8) further includes electromagnetism Iron (82), the electromagnet (82) is two and be separately positioned on the movable trolley (122) is located at second magnet plunger (81) on the position of two sides, microscope carrier (2) correspond to the electromagnet (82) side wall on be formed with for the electromagnet (82) the electromagnet latch (28) cooperated, in the lockup state, the electromagnet (82) is powered and the magnetic force by generating And be adsorbed onto the electromagnet latch (28), with microscope carrier described in locking (2) and the movable trolley (122)；In the solution Lock status, the electromagnet (82) powers off and is detached from the electromagnet latch (28), to unlock the microscope carrier (2) and the work Dynamic trolley (122).
27. sample processing device according to claim 26, which is characterized in that the microscope carrier transport establishment (1) includes setting It sets on the side inner sidewall of the working region of the microscope carrier transport establishment (1) and along the microscope carrier moving direction (Z1) extension Second positioning seat (15), with for the microscope carrier (2) to be navigated to each working region, on second positioning seat (15) Multiple second positioning regions are positioned apart from along the microscope carrier moving direction (Z1), second positioning region is used for and microscope carrier (2) The side side wall on be arranged the first positioning region cooperation or separation, in the lockup state, all second positioning regions With first position portion from so that the microscope carrier (2) is in the state of with described movable trolley (122) locking in institute It states and is moved freely on upper layer microscope carrier shipping platform (111), in the unlocked state, first positioning region is located at and described second When the opposite position in the second positioning region of any one in positioning region, first positioning region is matched with corresponding second positioning region It closes, is put down so that the microscope carrier (2) navigates to the upper layer microscope carrier transport in the state of unlocking with the movable trolley (122) On platform (111).
28. sample processing device according to claim 27, which is characterized in that second positioning region is the first magnet plunger (151), first positioning region is locating slot (221), and first magnet plunger (151) has working condition and inoperative shape State, in the working condition, the first magnet plunger (151) power-off, so that the push rod of the first magnet plunger (151) stretches out and blocks Enter into the locating slot (221), in the off working state, first magnet plunger (151) is powered, to pass through the first electromagnetism The magnetic force generated in bar (151) makes the pusher retracted and is detached from the locating slot (221).
29. sample processing device according to claim 28, which is characterized in that the side side wall of the microscope carrier (2) On be provided with the first positioning seat (22), the locating slot (221) is formed on first positioning seat (22), it is described second positioning On the position of neighbouring each first magnet plunger (151) of seat (15), it is separately provided for detecting first positioning seat (22) position sensor of position detects described first with a position sensor in multiple position sensors When the position of positioning seat (22), so that corresponding to the first electricity of one position sensor in first magnet plunger (151) Magnetic bar and the locating slot (221) cooperate.
30. sample processing device according to claim 29, which is characterized in that the position sensor is groove profile photopotential It sets sensor (16), the slot of the groove profile photoelectrical position sensor (16) is towards the interior of the upper layer microscope carrier shipping platform (111) Side opening is formed with for the slot across the groove profile photoelectrical position sensor (16) on first positioning seat (22) Block (222).
31. sample processing device according to claim 14, which is characterized in that microscope carrier lifting driving structure (13) packet It includes the lifting fixed pedestal (131) being arranged on lower layer's microscope carrier shipping platform (112) and the liter is liftably set The lift cylinders (132) that drops on fixed pedestal (131) and can be detached from the microscope carrier (2) cooperation, so that microscope carrier (2) is in institute State switching position between upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112).
32. sample processing device according to claim 31, which is characterized in that lower layer's microscope carrier shipping platform (112) Inner sidewall on be provided with microscope carrier return driving structure (18), the microscope carrier return driving structure (18) and the lifting fixed pedestal (131) it connects, and for driving microscope carrier lifting driving structure (13) along microscope carrier moving direction (Z1) or in contrast to the load The direction of platform moving direction (Z1) is mobile.
33. sample processing device according to claim 32, which is characterized in that microscope carrier return driving structure (18) packet It includes the second driving motor (181) and is driven the second transmission component being connected with the output shaft of second driving motor (181), it is described Lifting fixed pedestal (131) is mounted on the movable part of second transmission component, and second transmission component is used for will be described The rotary motion of second driving motor (181) is converted to microscope carrier lifting driving structure (13) along the microscope carrier moving direction (Z1) linear movement, or the linear movement in the direction in contrast to the microscope carrier moving direction (Z1).
34. sample processing device according to claim 33, which is characterized in that second transmission component is matched for V belt translation Close mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission matching mechanism.
35. sample processing device according to claim 34, which is characterized in that second transmission component is matched for V belt translation Mechanism is closed, and the second transmission belt (182) including connecting with lifting fixed pedestal (131), setting are in second transmission With (182) side and with the second driving wheel (183) of the output axis connection of second driving motor (181), setting described Second driven wheel (184) of the second transmission belt (182) other side, the lifting fixed pedestal (131) are fixed on described second and pass On dynamic band (182).
36. sample processing device according to claim 35, which is characterized in that second transmission component is provided with two groups And be separately positioned on the two sides inner wall of lower layer's microscope carrier shipping platform (112), two groups of second driven wheel (184) is logical Transmission shaft (185) is crossed mutually to interlock.
37. sample processing device according to claim 36, which is characterized in that lower layer's microscope carrier shipping platform (112) Two sides inner wall in be located at second transmission belt (182) below position on, be respectively arranged with along microscope carrier moving direction (Z1) The lifting fixed pedestal guide rail (133) for extending and cooperating with the two sidewalls of lifting fixed pedestal (131), so that the liter Drop fixed pedestal (131) drives the microscope carrier (2) to move on the lifting fixed pedestal guide rail (133) and make the microscope carrier (2) the initial storage area of the microscope carrier (D) is returned back to.
CN201821212593.8U 2018-07-27 2018-07-27 Sample processing device Active CN208902743U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207341A (en) * 2018-07-27 2019-01-15 山东见微生物科技有限公司 sample processing device and sample processing method
CN111588414A (en) * 2020-05-22 2020-08-28 珠海市人民医院 Bifurcation Avoidance Long Sampling Bar
CN115427780A (en) * 2020-02-27 2022-12-02 莫拉雷研究公司 Automatic biological sample extraction system and device
WO2023050207A1 (en) * 2021-09-29 2023-04-06 京东方科技集团股份有限公司 Detection system

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207341A (en) * 2018-07-27 2019-01-15 山东见微生物科技有限公司 sample processing device and sample processing method
CN115427780A (en) * 2020-02-27 2022-12-02 莫拉雷研究公司 Automatic biological sample extraction system and device
CN111588414A (en) * 2020-05-22 2020-08-28 珠海市人民医院 Bifurcation Avoidance Long Sampling Bar
CN111588414B (en) * 2020-05-22 2022-05-13 珠海市人民医院 Bifurcation Avoidance Long Sampling Bar
WO2023050207A1 (en) * 2021-09-29 2023-04-06 京东方科技集团股份有限公司 Detection system

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