CN208898908U - Microorganism and the dual-purpose bioreactor of zooblast - Google Patents
Microorganism and the dual-purpose bioreactor of zooblast Download PDFInfo
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- CN208898908U CN208898908U CN201821574796.1U CN201821574796U CN208898908U CN 208898908 U CN208898908 U CN 208898908U CN 201821574796 U CN201821574796 U CN 201821574796U CN 208898908 U CN208898908 U CN 208898908U
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- tank body
- rabbling mechanism
- zooblast
- installation axle
- agitating shaft
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Abstract
The utility model discloses a kind of microorganisms and the dual-purpose bioreactor of zooblast, including tank body, ventilation component, mixing component, monitoring assembly, perfusion component, control assembly, mixing component includes driving motor, agitating shaft and rabbling mechanism, one end of the mixing shaft and driving motor are sequentially connected, device for sealing magnetic fluid is provided between agitating shaft and tank body, a plurality of installation axle sleeves are additionally provided on agitating shaft, the position that installation axle is placed on the agitating shaft is adjustable；Rabbling mechanism is detachably arranged in installation axle and puts on, the tilt adjustable of rabbling mechanism；And a plurality of baffles along tank body circumferential array are additionally provided in tank body, baffle is detachably arranged on agitating shaft.Microorganism provided by the utility model and the dual-purpose bioreactor of zooblast can realize that microorganism and zooblast are collinearly cultivated, and avoid idleness of equipment waste；And corresponding reactor apparatus need not be bought for certain cell characteristics, reduce production or research and development cost.
Description
Technical field
The utility model relates to a kind of bioreactor, in particular to a kind of microorganism and the dual-purpose biological respinse of zooblast
Device belongs to cell cultivation equipment technical field.
Background technique
Currently, common cell expression system is main are as follows: microorganism and zooblast.The cell of both types is to reaction
The ratio of height to diameter of device, stirring, baffle, temperature control, DO control mode, control of additive raw material mode etc. have respective requirement.Existing market
Reactor be mostly personalized reactor, can only meet the needs of one or more of cell techniques, have following defects that
(1) specific function needed for reactor can only meet certain or several cells, can not be public, causes the idleness of equipment unrestrained
Take；
(2) when production technology is not known, the process requirements that more reactor apparatus meet different cells need to be bought, or
Inappropriate reactor has been purchased, research and development or production cost are increased.
Utility model content
In view of the deficiencies of the prior art, the main purpose of the utility model is to provide a kind of microorganisms and zooblast two
Use bioreactor.
For the aforementioned purpose of utility model of realization, the technical solution adopted in the utility model includes:
The utility model embodiment provides a kind of microorganism and the dual-purpose bioreactor of zooblast, including is at least used for
Accommodate the tank body of culture solution and cover on Gai Heyu tank body can be opened, at least for be passed through into the tank body constant pressure without
The ventilation component of bacterium gas, it is at least described for monitoring at least for being stirred the mixing component of culture solution in the tank body
The monitoring assembly of culture environment in tank body at least for supplementing or the perfusion component of drain tank In vivo culture liquid, and is at least used
In the control assembly for controlling each component operating status in the reactor, the ventilation component, mixing component, monitoring assembly and filling
Note component is also connect with the control assembly；The mixing component includes driving motor, agitating shaft and rabbling mechanism, described
One end of the mixing shaft and driving motor are sequentially connected, and the other end is extended in tank body, is provided between the agitating shaft and tank body
Device for sealing magnetic fluid, is additionally provided with a plurality of installation axle sleeves on the agitating shaft, and the installation axle is placed on the agitating shaft
On position it is adjustable；The rabbling mechanism is detachably arranged in the installation axle and puts on, the tilt angle of the rabbling mechanism
It is adjustable；And a plurality of baffles along tank body circumferential array are additionally provided in the tank body, the baffle is removably installed
On agitating shaft.
Further, the axial direction along the agitating shaft is arranged at intervals with a plurality of limits to match with installation axle sleeve
Slot, wherein the limiting slot is continuous arc groove, the inner wall of the installation axle sleeve is the company to match with the limiting slot
Continuous arc convex.
Further, the installation axle, which is put on, is additionally provided with the first threaded hole that radially direction extends to form, institute
It states and is provided with the first threaded fastener in the first threaded hole, the installation axle sleeve can be consolidated via first threaded fastener
Due on agitating shaft；The installation axle put on be additionally provided with more than two fixed hole positions to install the rabbling mechanism and
The second threaded hole being connected to fixed hole position is provided with the second threaded fastener in second threaded hole, via described second
Rabbling mechanism can be fixed on installation axle and put on by threaded fastener.
Further, the rabbling mechanism includes the first rabbling mechanism, and first rabbling mechanism includes edge more than two
The spaced agitating paddle of the axial direction of agitating shaft.
Further, the agitating paddle includes blade and the connecting rod for being set to blade side, and the connecting rod can
It is removably inserted into the fixed hole position of the installation axle sleeve.
Further, the rabbling mechanism further includes the second rabbling mechanism, and the second rabbling mechanism is set to the first stirring
Above mechanism, second rabbling mechanism includes defoaming paddle.
Further, a plurality of baffles are fixed on fixed bracket, and the baffle is fixed on fixed bracket, described
Fixed bracket is detachably arranged on agitating shaft.
Further, collet water inlet and collet water outlet are additionally provided on the tank body.
Further, the ventilation component includes air inlet, gas distributor, air inlet pipe, escape pipe and gas outlet, described
Air inlet is connected through air inlet pipe with gas distributor；The monitoring assembly includes temperature-controlling module, dissolved oxygen control assembly, pH
Control assembly and defoaming component；The temperature-controlling module includes temperature sensor for monitoring culture-liquid temp and sets
It is placed in the temperature-adjusting device of the tank wall；The dissolved oxygen control assembly includes the dissolved oxygen electricity for monitoring culture solution dissolved oxygen
Pole；The pH control assembly includes that the acid/base liquid for monitoring the pH electrode of medium pH value and being connected to the tank inner chamber is mended
Add pipeline and peristaltic pump；It is described defoaming component include monitor culture solution foam position level electrode and with the tank inner chamber
The defoaming agent of connection adds pipeline and peristaltic pump.
Further, surface layer blow vent, deep ventilation mouth, tail gas condenser, two or more are provided on the cover
Perfusing hole, defoaming electrode ports, temperature electrode mouth, pH electrode ports, DO electrode ports, soda acid material-feeding port, at least one spare mouth；?
Gasket seal is provided between the cover and tank body.
Compared with prior art, microorganism provided by the utility model and the dual-purpose bioreactor of zooblast, structure letter
Single, technical ability is used for animal cell culture, can be used for microbial cell culture, the drawbacks of overcoming in the prior art；And this reality
It can realize that microorganism and zooblast are collinearly cultivated with the microorganism of novel offer and the dual-purpose bioreactor of zooblast, avoid
Idleness of equipment waste；And corresponding reactor apparatus need not be bought for certain cell characteristics, reduce production or research and development at
This.
Detailed description of the invention
Fig. 1 is a kind of knot of microorganism and the dual-purpose bioreactor of zooblast in one exemplary embodiments of the utility model
Structure schematic diagram；
Fig. 2 is that a kind of microorganism and the dual-purpose bioreactor of zooblast are used in one exemplary embodiments of the utility model
Structural schematic diagram under animal cell culture；
Fig. 3 is that a kind of microorganism and the dual-purpose bioreactor of zooblast are used in one exemplary embodiments of the utility model
Structural schematic diagram under microculture.
Specific embodiment
In view of deficiency in the prior art, inventor is studied for a long period of time and is largely practiced, and is able to propose that this is practical new
The technical solution of type.The technical solution, its implementation process and principle etc. will be further explained as follows.
The utility model embodiment provides a kind of microorganism and the dual-purpose bioreactor of zooblast, including is at least used for
Accommodate the tank body of culture solution and cover on Gai Heyu tank body can be opened, at least for be passed through into the tank body constant pressure without
The ventilation component of bacterium gas, it is at least described for monitoring at least for being stirred the mixing component of culture solution in the tank body
The monitoring assembly of culture environment in tank body at least for supplementing or the perfusion component of drain tank In vivo culture liquid, and is at least used
In the control assembly for controlling each component operating status in the reactor, the ventilation component, mixing component, monitoring assembly and filling
Note component is also connect with the control assembly；The mixing component includes driving motor, agitating shaft and rabbling mechanism, described
One end of the mixing shaft and driving motor are sequentially connected, and the other end is extended in tank body, is provided between the agitating shaft and tank body
Device for sealing magnetic fluid, is additionally provided with a plurality of installation axle sleeves on the agitating shaft, and the installation axle is placed on the agitating shaft
On position it is adjustable；The rabbling mechanism is detachably arranged in the installation axle and puts on, the tilt angle of the rabbling mechanism
It is adjustable；And a plurality of baffles along tank body circumferential array are additionally provided in the tank body, the baffle is removably installed
On agitating shaft.
Further, the axial direction along the agitating shaft is arranged at intervals with a plurality of limits to match with installation axle sleeve
Slot, wherein the limiting slot is continuous arc groove, the inner wall of the installation axle sleeve is the company to match with the limiting slot
Continuous arc convex.
Further, the installation axle, which is put on, is additionally provided with the first threaded hole that radially direction extends to form, institute
It states and is provided with the first threaded fastener in the first threaded hole, the installation axle sleeve can be consolidated via first threaded fastener
Due on agitating shaft；The installation axle put on be additionally provided with more than two fixed hole positions to install the rabbling mechanism and
The second threaded hole being connected to fixed hole position is provided with the second threaded fastener in second threaded hole, via described second
Rabbling mechanism can be fixed on installation axle and put on by threaded fastener.
Further, the rabbling mechanism includes the first rabbling mechanism, and first rabbling mechanism includes edge more than two
The spaced agitating paddle of the axial direction of agitating shaft.
Further, the agitating paddle includes blade and the connecting rod for being set to blade side, and the connecting rod can
It is removably inserted into the fixed hole position of the installation axle sleeve.
Further, the rabbling mechanism further includes the second rabbling mechanism, and the second rabbling mechanism is set to the first stirring
Above mechanism, second rabbling mechanism includes defoaming paddle.
Further, a plurality of baffles are fixed on fixed bracket, and the baffle is fixed on fixed bracket, described
Fixed bracket is detachably arranged on agitating shaft.
Further, collet water inlet and collet water outlet are additionally provided on the tank body.
Further, the ventilation component includes air inlet, gas distributor, air inlet pipe, escape pipe and gas outlet, described
Air inlet is connected through air inlet pipe with gas distributor；The monitoring assembly includes temperature-controlling module, dissolved oxygen control assembly, pH
Control assembly and defoaming component；The temperature-controlling module includes temperature sensor for monitoring culture-liquid temp and sets
It is placed in the temperature-adjusting device of the tank wall；The dissolved oxygen control assembly includes the dissolved oxygen electricity for monitoring culture solution dissolved oxygen
Pole；The pH control assembly includes that the acid/base liquid for monitoring the pH electrode of medium pH value and being connected to the tank inner chamber is mended
Add pipeline and peristaltic pump；It is described defoaming component include monitor culture solution foam position level electrode and with the tank inner chamber
The defoaming agent of connection adds pipeline and peristaltic pump.
Further, surface layer blow vent, deep ventilation mouth, tail gas condenser, two or more are provided on the cover
Perfusing hole, defoaming electrode ports, temperature electrode mouth, pH electrode ports, DO electrode ports, soda acid material-feeding port, at least one spare mouth；?
Gasket seal is provided between the cover and tank body.
The technical solution, its implementation process and principle etc. will be further explained in conjunction with attached drawing as follows.
Referring to Fig. 1, a kind of microorganism and the dual-purpose bioreactor of zooblast, including at least for accommodating culture solution
Tank body 1 and cover 2 on Gai Heyu tank body can be opened, at least for being passed through the logical of constant pressure aseptic gas into the tank body
Pneumatic module, at least for being stirred the mixing component of culture solution in the tank body, at least for monitoring the tank In vivo culture
The monitoring assembly of environment, at least for supplement or the perfusion component of drain tank In vivo culture liquid, and at least for described in controlling
The control assembly of each component operating status in reactor, the ventilation component, mixing component, monitoring assembly and perfusion component are also equal
It is connect with the control assembly；The ventilation component includes deep ventilation mechanism 10, air inlet, gas distributor, air inlet pipe, goes out
Tracheae and gas outlet, the air inlet are connected through air inlet pipe with gas distributor；The monitoring assembly includes temperature control group
Part, dissolved oxygen control assembly, pH control assembly and defoaming component；The temperature-controlling module includes for monitoring culture-liquid temp
Temperature sensor and be set to the temperature-adjusting device of the tank wall；The dissolved oxygen control assembly includes for monitoring
The dissolved oxygen electrode of culture solution dissolved oxygen；The pH control assembly include monitor medium pH value pH electrode and in the tank body
The acid/base liquid of chamber connection adds pipeline and peristaltic pump；The defoaming component includes the level electrode for monitoring culture solution foam position
And the defoaming agent being connected to the tank inner chamber adds pipeline and peristaltic pump；It is logical that surface layer blow vent, deep layer are provided on cover
Port, tail gas condenser, more than two perfusing holes, defoaming electrode ports, temperature electrode mouth, pH electrode ports, D0 electrode ports, soda acid
Material-feeding port, at least one spare mouth, gasket seal 3 is provided between tank body 1 and cover 2, folder is additionally provided on the outside of tank body
Water outlet 11, collet water inlet 12 are covered, the setting of collet water inlet 12 is arranged on tank body in tank body lower part, collet water outlet 11
Portion.
Mixing component includes that driving motor 4 and the agitating shaft 8 with the transmission connection of driving motor 4, driving motor 4 are mounted on
On electric machine support 6, one end of agitating shaft 8 is connect through shaft coupling 5 with driving motor 4, and the other end extends in tank body, in agitating shaft
It is provided with device for sealing magnetic fluid 7 between 8 and tank body 1, a plurality of installation axle sleeves are additionally provided on agitating shaft, installation axle is placed on
Position on agitating shaft is adjustable；Installation axle, which is put on, is removably equipped with rabbling mechanism；The installation axle, which is put on, is additionally provided with edge
The first threaded hole that its radial direction extends to form is provided with the first threaded fastener in first threaded hole, via described
The installation axle sleeve can be fixed on agitating shaft by the first threaded fastener；The installation axle, which is put on, is additionally provided with two or more
To the second threaded hole installing the fixed hole position of the rabbling mechanism and being connected to fixed hole position, in second threaded hole
It is provided with the second threaded fastener, rabbling mechanism installation axle can be fixed on via second threaded fastener and put on；With
And fixed bracket 91 is also detachably connected on agitating shaft 8, a plurality of baffles 9 are fixedly connected on fixed bracket, plural number
A baffle 9 is distributed around the circumferential array of tank body 1；A plurality of and installation axle sleeve is arranged at intervals with along the axial direction of agitating shaft 8
The limiting slot 801 to match, wherein limiting slot 801 is continuous arc groove, and the inner wall for installing axle sleeve is and the limiting slot phase
Matched continuous arc convex.
Referring to Fig. 2, rabbling mechanism be the spaced agitating paddle 81,82 of two groups of axial directions along agitating shaft, two groups
Agitating paddle 81,82 is as ear paddle, and agitating paddle 81 is arranged above agitating paddle 82, and every group of agitating paddle includes three blades, each paddle
Piece includes blade and the connecting rod for being set to blade side, and connecting rod can be removably inserted into the fixed hole position of installation axle sleeve
It is interior.
Referring to Fig. 3, rabbling mechanism is the spaced agitating paddle 81,82 of two groups of axial directions along agitating shaft and disappears
Steep paddle 83；Two groups of agitating paddles 81,82 are six leaf vane wheel oars, and agitating paddle 81 is arranged above agitating paddle 82, and every group of agitating paddle includes
Three blades, each blade include blade and the connecting rod for being set to blade side, and connecting rod can be removably inserted into installation
In the fixed hole position of axle sleeve；The blade lower part of defoaming paddle 83 is provided with zigzag structure or flat impeller structure or orthogonal round bar
With the composite structure of thin bar.
Compared with prior art, microorganism provided by the utility model and the dual-purpose bioreactor of zooblast, structure letter
Single, technical ability is used for animal cell culture, can be used for microbial cell culture, the drawbacks of overcoming in the prior art；And this reality
It can realize that microorganism and zooblast are collinearly cultivated with the microorganism of novel offer and the dual-purpose bioreactor of zooblast, avoid
Idleness of equipment waste；And corresponding reactor apparatus need not be bought for certain cell characteristics, reduce production or research and development at
This.
It should be appreciated that above-described embodiment is only to illustrate the technical ideas and features of the present invention, it is ripe its object is to allow
The personage for knowing technique can understand the content of the utility model and implement accordingly, can not limit the utility model with this
Protection scope.All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the utility model
Within protection scope.
Claims (9)
1. a kind of microorganism and the dual-purpose bioreactor of zooblast, including at least for accommodating culture solution tank body and can
The cover on Gai Heyu tank body is opened at least to use at least for being passed through the ventilation component of constant pressure aseptic gas into the tank body
In the mixing component for being stirred culture solution in the tank body, at least for monitoring the monitoring group of culture environment in the tank body
Part at least for supplement or the perfusion component of drain tank In vivo culture liquid, and is at least used to control each group in the reactor
The control assembly of part operating status, the ventilation component, mixing component, monitoring assembly and perfusion component also with the control group
Part connection；It is characterized by: the mixing component includes driving motor, agitating shaft and rabbling mechanism, the one end of the mixing shaft
It is sequentially connected with driving motor, the other end extends in tank body, is provided with magnet fluid sealing between the agitating shaft and tank body
Device is additionally provided with a plurality of installation axle sleeves on the agitating shaft, and the position that the installation axle is placed on the agitating shaft can
It adjusts；The rabbling mechanism is detachably arranged in the installation axle and puts on, the tilt adjustable of the rabbling mechanism；And
A plurality of baffles along tank body circumferential array are additionally provided in the tank body, the baffle is detachably arranged on agitating shaft.
2. microorganism according to claim 1 and the dual-purpose bioreactor of zooblast, it is characterised in that: along the stirring
The axial direction of axis is arranged at intervals with a plurality of limiting slots to match with installation axle sleeve, wherein the limiting slot is continuous arc
The inner wall of connected in star, the installation axle sleeve is the continuous arc convex to match with the limiting slot.
3. microorganism according to claim 2 and the dual-purpose bioreactor of zooblast, it is characterised in that: the installation axle
It puts on and is additionally provided with the first threaded hole that radially direction extends to form, it is tight to be provided with the first screw thread in first threaded hole
The installation axle sleeve can be fixed on agitating shaft by firmware via first threaded fastener；The installation axle is put on also
It is provided with more than two the second threaded holes to install the fixed hole position of the rabbling mechanism and be connected to fixed hole position, institute
It states and is provided with the second threaded fastener in the second threaded hole, rabbling mechanism can be fixed on via second threaded fastener
Installation axle is put on.
4. microorganism according to claim 3 and the dual-purpose bioreactor of zooblast, it is characterised in that: the blender
Structure includes the first rabbling mechanism, and first rabbling mechanism, which includes that the axial direction more than two along agitating shaft is spaced, to be stirred
Mix paddle；And/or the agitating paddle includes blade and the connecting rod for being set to blade side, the connecting rod can be detachable
Ground is inserted into the fixed hole position of the installation axle sleeve.
5. microorganism according to claim 4 and the dual-purpose bioreactor of zooblast, it is characterised in that: the blender
Structure further includes the second rabbling mechanism, and the second rabbling mechanism is set to above the first rabbling mechanism, and second rabbling mechanism includes
Defoam paddle.
6. microorganism according to claim 1 and the dual-purpose bioreactor of zooblast, it is characterised in that: a plurality of described
Baffle is fixed on fixed bracket, and the baffle is fixed on fixed bracket, and the fixed bracket is detachably arranged in stirring
On axis.
7. microorganism according to claim 1 and the dual-purpose bioreactor of zooblast, it is characterised in that: in the tank body
On be additionally provided with collet water inlet and collet water outlet.
8. microorganism according to claim 1 and the dual-purpose bioreactor of zooblast, it is characterised in that: the ventilation group
Part includes air inlet, gas distributor, air inlet pipe, escape pipe and gas outlet, and the air inlet is through air inlet pipe and gas distributor
It is connected；The monitoring assembly includes temperature-controlling module, dissolved oxygen control assembly, pH control assembly and defoaming component；It is described
Temperature-controlling module includes temperature sensor for monitoring culture-liquid temp and the temperature tune for being set to the tank wall
Regulating device；The dissolved oxygen control assembly includes the dissolved oxygen electrode for monitoring culture solution dissolved oxygen；The pH control assembly includes prison
The acid/base liquid controlling the pH electrode of medium pH value and being connected to the tank inner chamber adds pipeline and peristaltic pump；The defoaming
Component include the level electrode for monitoring culture solution foam position and the defoaming agent that is connected to the tank inner chamber add pipeline and
Peristaltic pump.
9. microorganism according to claim 8 and the dual-purpose bioreactor of zooblast, it is characterised in that: on the cover
It is provided with surface layer blow vent, deep ventilation mouth, tail gas condenser, more than two perfusing holes, defoaming electrode ports, temperature electrode
Mouth, pH electrode ports, D0 electrode ports, soda acid material-feeding port, at least one spare mouth；Sealing is provided between the cover and tank body
Gasket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821574796.1U CN208898908U (en) | 2018-09-26 | 2018-09-26 | Microorganism and the dual-purpose bioreactor of zooblast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821574796.1U CN208898908U (en) | 2018-09-26 | 2018-09-26 | Microorganism and the dual-purpose bioreactor of zooblast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208898908U true CN208898908U (en) | 2019-05-24 |
Family
ID=66573731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821574796.1U Active CN208898908U (en) | 2018-09-26 | 2018-09-26 | Microorganism and the dual-purpose bioreactor of zooblast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208898908U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113667595A (en) * | 2021-08-11 | 2021-11-19 | 安徽霍尔斯工程技术有限公司 | Moving bed bioreactor |
| WO2021238286A1 (en) * | 2020-05-26 | 2021-12-02 | 上海艾众生物科技有限公司 | Gas introducing device for bioreactor |
| CN114561291A (en) * | 2022-03-21 | 2022-05-31 | 广州齐志生物工程设备有限公司 | 500ml small-sized one-control multi-cell bioreactor |
-
2018
- 2018-09-26 CN CN201821574796.1U patent/CN208898908U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021238286A1 (en) * | 2020-05-26 | 2021-12-02 | 上海艾众生物科技有限公司 | Gas introducing device for bioreactor |
| CN113667595A (en) * | 2021-08-11 | 2021-11-19 | 安徽霍尔斯工程技术有限公司 | Moving bed bioreactor |
| CN114561291A (en) * | 2022-03-21 | 2022-05-31 | 广州齐志生物工程设备有限公司 | 500ml small-sized one-control multi-cell bioreactor |
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Effective date of registration: 20221101 Address after: 226200 No.500, Linyang Road, Qidong Economic Development Zone, Nantong City, Jiangsu Province Patentee after: Wotisi (Nantong) Biotechnology Co.,Ltd. Address before: 215000 No.81 Suli Road, Wuzhong District, Suzhou City, Jiangsu Province Patentee before: SUZHOU DIOUYI BIOTECHNOLOGY CO.,LTD. |