CN208537230U - A kind of iron shot sample injector and the porous sampling plate comprising iron shot sample injector - Google Patents

Abstract

The utility model relates to a kind of iron shot sample injector and include the porous sampling plate of iron shot sample injector, iron shot sample injector includes sample injector ontology and the sample-adding bushing for being set to sample injector body bottom portion, the two surrounds iron shot receiving portion, being loaded bushing includes the first, second, third bushing for being sequentially overlapped arrangement from top to bottom, it is respectively arranged with the first, second, and third leak hole thereon, when initial position, first leak hole and the second leak hole parallel misalignment, second leak hole and third leak hole are conducted, and institute's iron shot is located in the first leak hole；When iron shot operating position is added, by mobile second bushing, the first leak hole is conducted with the second leak hole, and the second leak hole and third leak hole parallel misalignment, iron shot are fallen into the second leak hole；When returning to initial position, second bushing is set to reset to initial position by mobile, iron shot falls into third leak hole and falls into the sampling hole of porous sampling plate.The utility model greatly shortens the experimental implementation time, and leakage plus iron shot situation will not occur.

Description

A kind of iron shot sample injector and the porous sampling plate comprising iron shot sample injector

Technical field

The utility model relates to a kind of DNA of plants samplers, and in particular to a kind of iron shot sample injector and add comprising iron shot The porous sampling plate of sample device.

Background technique

Disrupting tissue cell, exposure nucleic acid, it is generally the case that can use mortar tissue is thin are needed when extracting DNA of plants Born of the same parents are broken, and extracting solution is added and carries out DNA extraction.But when sample size is more, with mortar, sample is ground one by one, time and effort consuming.

And sample is taken into porous sampling plate, sample and iron shot is added in each hole, utilizes liquid nitrogen frozen, machinery shake It swings, so that the iron shot shaken is smashed histocyte and extract DNA, can once extract the DNA of multiple samples in this way, it is time saving and energy saving, It is more efficient.Currently used is 96 hole sampling plates.However, small iron shot need to be added one by one in 96 holes in experimental implementation, 2 iron shots are added in each hole, take the small iron shot of 3mm or so to be not easy very much with hand, and are easy to happen leakage plus iron shot in some hole Situation causes extraction DNA concentration low, influences whether subsequent experimental since leakage plus iron shot cannot sufficiently be crushed plant tissue cell Success or failure, with hand plus iron shot this process not only it is slow be easy to appear again leakage plus, not only having influenced conventional efficient, success or failure are tested in influence again, for Above situation, it would be highly desirable to which a kind of sampler that can effectively solve problem above is provided.

Utility model content

The utility model is intended to provide a kind of iron shot sample injector that can accurately and efficiently can add iron shot into porous sampling plate With the porous sampling plate comprising iron shot sample injector.

A kind of iron shot sample injector of the utility model, for iron shot, feature to be added into the sampling hole of porous sampling plate It is, including sample injector ontology and the sample-adding bushing for being set to sample injector body bottom portion, the sample injector ontology and the sample-adding Bushing surrounds iron shot receiving portion, the sample-adding bushing include the first bushing for being sequentially overlapped arrangement from top to bottom, the second bushing and Third bushing is respectively arranged with the first leak hole, the second leak hole on first bushing, second bushing and the third bushing With third leak hole, when initial position, first leak hole and the second leak hole parallel misalignment, second leak hole and described the Three leak holes are conducted, and institute's iron shot is located in the first leak hole；When iron shot operating position is added, pass through movement second bushing, institute It states the first leak hole to be conducted with second leak hole, the second leak hole and third leak hole parallel misalignment, iron shot are fallen into the second leak hole； When returning to initial position, second bushing is set to reset to initial position, first leak hole and second leakage by mobile Hole parallel misalignment, the second leak hole are conducted with third leak hole, and iron shot falls into third leak hole and falls into the sampling of porous sampling plate In hole.

Further, the iron shot sample injector of the utility model, first leak hole, second leak hole and third leakage The diameter in hole is slightly larger than iron shot diameter, the natural several times with a thickness of iron shot diameter of second bushing.

Further, the iron shot sample injector of the utility model, first leak hole, second leak hole and third leakage Hole is cylindricality leak hole.

Further, the iron shot sample injector of the utility model, first leak hole are infundibulate leak hole, second leak hole It is cylindricality leak hole with the third leak hole, the minimum-value aperture of the infundibulate leak hole is equal to the aperture of the cylindricality leak hole.

Further, the iron shot sample injector of the utility model is provided with reset spring on second bushing, for making plus The second bushing resets after sample operates.

Further, the iron shot sample injector of the utility model is provided with pushing hands on second bushing.

Further, the iron shot sample injector of the utility model, the top of the sample injector ontology are provided with lid, the lid Body is embedded slip lid.

Further, the iron shot sample injector of the utility model is provided with positioning protrusion on the lid.

Include the porous sampling plate of iron shot sample injector another object of the present invention is to provide a kind of, including takes up an official post one by one Kind iron shot sample injector, the iron shot sample injector are set on the porous sampling plate, and the porous sampling plate is that 96 sampling holes take Template.

Further, the utility model include iron shot sample injector porous sampling plate, the iron shot sample injector and institute It states and is detachably fixed connection on porous sampling plate.

The utility model has the advantages that the iron shot sample injector of the utility model and the porous sampling plate for including iron shot sample injector, only need Iron shot disposably can be filled it up with the porous of porous sampling plate by once-through operation, greatly shorten the experimental implementation time, and will not occur Leakage plus iron shot situation, improve conventional efficient and DNA success rate of extracting.

Detailed description of the invention

Fig. 1, the utility model include the porous sample-adding plate structural schematic diagram of iron shot sample injector.

The porous sample-adding plate structural schematic diagram of Fig. 2, the utility model.

Fig. 3, the utility model iron shot sample injector the first bushing top view.

Fig. 4, the utility model iron shot sample injector the second bushing top view.

Fig. 5, the utility model iron shot sample injector third bushing top view.

Appended drawing reference: 1, porous sample-adding plate, 11, sampling hole, 2, iron shot sample injector ontology, 3, sample-adding bushing, the 31, first leakage Plate, the 32, second bushing, 33, third bushing, the 34, first leak hole, the 35, second leak hole, 36, third leak hole, 37, pushing hands, 38, multiple Position spring, 21, iron shot receiving portion, 22, embedded slip lid, 221, positioning protrusion.

Specific embodiment

The application is further elaborated below in conjunction with specification drawings and specific embodiments.

As shown in Fig. 1 to 5, the iron shot sample injector of the utility model is set on porous sampling plate 1, iron shot sample injector and more Connection is detachably fixed on hole sampling plate 1.Being fixedly connected can allow the two more firm, for example can be insert-type, buckle-type etc., For example buckle-type can be designed to position limiting structure card slot, clamp in junction, be directly placed at the two together.

A kind of iron shot sample injector of the utility model, it is special for iron shot to be added into the sampling hole 11 of porous sampling plate 1 Sign is, including sample injector ontology 2 and the sample-adding bushing 3 for being set to sample injector body bottom portion, the sample injector ontology 2 and described Sample-adding bushing 3 surrounds iron shot receiving portion 21, the sample-adding bushing 3 include the first bushing 31 for being sequentially overlapped arrangement from top to bottom, Second bushing 32 and third bushing 33 are set respectively on first bushing 31, second bushing 32 and the third bushing 33 It is equipped with the first leak hole 34, the second leak hole 35 and third leak hole 36, when initial position, first leak hole 34 and second leak hole 35 parallel misalignments, second leak hole 35 and the third leak hole 36 are conducted, and institute's iron shot is located in the first leak hole 34；Iron is added When pearl operating position, by movement second bushing 32, first leak hole 34 is conducted with second leak hole 35, and second Leak hole 35 and 36 parallel misalignment of third leak hole, iron shot are fallen into the second leak hole 35；When returning to initial position, by described in mobile make Second bushing 35 resets to initial position, first leak hole 34 and 35 parallel misalignment of the second leak hole, the second leak hole 35 with Third leak hole 36 is conducted, and iron shot falls into third leak hole 36 and falls into the sampling hole 11 of porous sampling plate 1.Preferably, iron Pearl receiving portion 21 is set as biggish volume, loads more iron shots.

Further, the iron shot sample injector of the utility model, first leak hole 34, second leak hole 35 and described The diameter of three leak holes 36 is slightly larger than iron shot diameter, the natural several times with a thickness of iron shot diameter of second bushing 32.Leak hole Diameter is in order to avoid the diameter of leak hole is more much larger than iron shot diameter slightly larger than iron shot diameter, and when use multiple iron shots occurs together Be stuck in the hole between bushing, cannot flexibly pushing plate B the case where.

Further, the iron shot sample injector of the utility model, first leak hole 34, second leak hole 35 and described Three leak holes 36 are cylindricality leak hole.The cylindricality leak hole formation is not limited to circular hole, or other shapes.

Further, the iron shot sample injector of the utility model, first leak hole 34 are infundibulate leak hole, second leakage Hole 35 and the third leak hole 36 are cylindricality leak hole, and the minimum-value aperture of the infundibulate leak hole is equal to the hole of the cylindricality leak hole Diameter.It can make to leak under iron shot more smoothly.

Further, the iron shot sample injector of the utility model is provided with reset spring 38 on second bushing 32, is used for The second bushing 32 resets after operating sample-adding.Reset spring can of course be not added, pass through manual left and right Quick push/pull second Bushing 32, also achievable iron shot falls；It may be designed in electric drive, one-key start push pull maneuver.

Further, the iron shot sample injector of the utility model is provided with push and pull bar 37 on second bushing 32.It is convenient Operation.

Further, the iron shot sample injector of the utility model, the top of the sample injector ontology are provided with lid, the lid Body is embedded slip lid 22.

Further, the iron shot sample injector of the utility model is provided with positioning protrusion 221 on the lid.Lid design For embedded slip lid 22, by positioning protrusion 221 can well stable lid it is not easily to fall off, prevent iron shot from iron shot receiving portion It spreads out and in 21, and Easy-to-open cover body, continue and add iron shot.

Specifically, include iron shot sample injector 96 hole sampling plate specific sizes design it is as follows, 96 hole sampling plates are long 126mm, wide 84mm, iron shot sample injector size specification is just to be buckled on 96 hole sampling plates, wherein sample-adding bushing 3 is arranged in iron The bottom of pearl sample injector ontology 2, lower section reserve smaller portions and are used to be buckled on 96 hole sampling plates.The sample-adding of iron shot sample injector leaks Plate 3 is 96 holes, and sample-adding bushing 3 includes the first bushing 31, the second bushing 32 and third bushing 33, the first bushing 31, the second bushing 32 and the first leak hole 34 on third bushing 33, the second leak hole 35 and the almost the same 3.5mm of 36 size of third leak hole, it is slightly larger than iron Pearl diameter 3.2mm, when initial position, 35 parallel misalignment of the first leak hole 34 and the second leak hole, the second leak hole 35 and third leak hole 36 It is completely coincident and is conducted, the iron shot part in iron shot receiving portion 21 rests in the first leak hole 34, and cannot continue to leak down；It is added When iron shot operating position, the second bushing 32 is pushed forward, make 96 the second leak holes 35 and the first bushing 31 of the second bushing 32 96 the first leak holes 34 are completely coincident and are conducted, 96 the second leak holes 35 of the second bushing 32 and 96 thirds of third bushing 33 36 parallel misalignment of leak hole, at this moment iron shot is out of, the first leak hole 34 falls to the second bushing 32 the second leak hole 35, and cannot continue Leak down, the second bushing 32 is under 38 rebound effect of reset spring, and rebound initial position, at this moment the second leakage on the second bushing 32 Hole 35 be restored to it is parallel with 96 the first leak holes 34 of the first bushing 31 staggeredly, it is complete with 96 third leak holes 36 of third bushing 33 Full weight conjunction is conducted, the third leak hole 36 that iron shot is fallen on third bushing 33 and the sampling hole 11 for directly falling into 96 hole sampling plates It is interior, it completes an iron shot and operation is added.If plate B thickness is equal to iron shot diameter, push a plate B that can leak down 1 granulated iron Pearl, repetitive operation can make to repeat that iron shot is added in each hole of sampling plate, and so on.The thickness of certain second bushing 32 It can be designed as other natural several times equal to iron shot diameter, push successively the second bushing 32, the iron for the corresponding times of grain that can leak down Pearl.In use, preferably being shaken when pushing the second bushing 32 to be overlapped the second leak hole 35 with the first leak hole 34 of the first bushing 31 Iron shot in dynamic iron shot receiving portion 21, it is therefore an objective to fall into iron shot sufficiently in the second leak hole 35 of the second bushing 32, avoid the occurrence of Iron shot is crowded mutually to be blocked, and cannot be fallen into the second leak hole 35 in time, be caused leakage plus iron shot situation.

Preferably, the first bushing 31, the second bushing 32 and the third bushing 33 for being loaded bushing 3 will be stacked together, and avoid There is larger gap between bushing, when avoiding leakage inter-plate gap larger, when pushing plate B activity, multiple iron shots occurs and be stuck in hole location, make plate B cannot flexibly function.The thickness of second bushing 32 should be the natural several times of iron shot diameter, avoid the second of the second bushing 32 When leak hole 35 is overlapped with the first leak hole 34 of the first bushing 31, extra iron shot part falls into the second leak hole 35 in the first leak hole 34 Interior, blocking the second leak hole 35 prevents the case where reset spring 38 is from springing back reset generation.

The utility model has the advantages that the iron shot sample injector of the utility model and the porous sampling plate for including iron shot sample injector, only need Iron shot disposably can be filled it up with the porous of porous sampling plate by once-through operation, greatly shorten the experimental implementation time, and will not occur Leakage plus iron shot situation, improve conventional efficient and DNA success rate of extracting.

The above is only the preferred embodiment of the present invention, it is all done according to present utility model application the scope of the patents it is equal Deng variation and modification, all belong to the range that the utility model is covered.

Claims (10)

1. a kind of iron shot sample injector, for iron shot to be added into the sampling hole of porous sampling plate, which is characterized in that including sample injector Ontology and the sample-adding bushing for being set to sample injector body bottom portion, the sample injector ontology and the sample-adding bushing surround iron shot receiving Portion, the sample-adding bushing include the first bushing, the second bushing and third bushing for being sequentially overlapped arrangement from top to bottom, and described first The first leak hole, the second leak hole and third leak hole, initial bit are respectively arranged on bushing, second bushing and the third bushing When setting, first leak hole and the second leak hole parallel misalignment, second leak hole and the third leak hole are conducted, institute's iron Pearl is located in the first leak hole；When iron shot operating position is added, by movement second bushing, first leak hole and described the Two leak holes are conducted, and the second leak hole and third leak hole parallel misalignment, iron shot are fallen into the second leak hole；When returning to initial position, lead to Crossing movement makes second bushing reset to initial position, first leak hole and the second leak hole parallel misalignment, the second leakage Hole is conducted with third leak hole, and iron shot falls into third leak hole and falls into the sampling hole of porous sampling plate.

2. iron shot sample injector according to claim 1, which is characterized in that first leak hole, second leak hole and institute The diameter of third leak hole is stated slightly larger than iron shot diameter, the natural several times with a thickness of iron shot diameter of second bushing.

3. iron shot sample injector according to claim 2, which is characterized in that first leak hole, second leak hole and institute Stating third leak hole is cylindricality leak hole.

4. iron shot sample injector according to claim 2, which is characterized in that first leak hole is infundibulate leak hole, described Second leak hole and the third leak hole are cylindricality leak hole, and the minimum-value aperture of the infundibulate leak hole is equal to the hole of the cylindricality leak hole Diameter.

5. iron shot sample injector according to claim 1, which is characterized in that it is provided with reset spring on second bushing, The second bushing resets after for operating sample-adding.

6. iron shot sample injector according to claim 1, which is characterized in that be provided with pushing hands on second bushing.

7. iron shot sample injector according to claim 1, which is characterized in that the top of the sample injector ontology is provided with lid Body, the lid are embedded slip lid.

8. iron shot sample injector according to claim 7, which is characterized in that be provided with positioning protrusion on the lid.

9. a kind of includes the porous sampling plate of iron shot sample injector, which is characterized in that including described in claim 1-8 any one Kind iron shot sample injector, the iron shot sample injector are set on the porous sampling plate, and the porous sampling plate is that 96 sampling holes take Template.

10. according to claim 9 includes the porous sampling plate of iron shot sample injector, which is characterized in that the iron shot adds Sample device and the porous sampling plate are to be detachably fixed connection.