CN208367324U - A kind of fluorescence microscope - Google Patents
A kind of fluorescence microscope Download PDFInfo
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- CN208367324U CN208367324U CN201820251492.5U CN201820251492U CN208367324U CN 208367324 U CN208367324 U CN 208367324U CN 201820251492 U CN201820251492 U CN 201820251492U CN 208367324 U CN208367324 U CN 208367324U
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- light source
- fluorescence
- fluorescence microscope
- optical filtering
- imaging device
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a kind of fluorescence microscopes, including light source, object lens, mirror holder, microscope base and objective table, the light source is mounted on the middle part upper end of the microscope base, fixed station and console are installed on the microscope base, the console top and the lower removable of the mirror holder are installed together, the top of the mirror holder is provided with movable objective apparatus, and the upper end of the activity objective apparatus is equipped with optical filtering, and the upper end of the optical filtering is equipped with imaging device.The light source generates the exciting light of specific wavelength, fluorescence sample illumination excitation is generated fluorescence corresponding with the wavelength of the exciting light by the exciting light, the optical filtering is used to come out the fluorescence filter for the specific wavelength for converging out by the object lens, obtain the single fluorescence of wavelength, to improve the accuracy of imaging, simultaneously, the single fluorescence of the wavelength is imaged by the imaging device, described image is shown by display screen amplification, and naked eyes is avoided to observe the inconvenience of image bring by eyepiece.
Description
Technical field
The utility model belongs to microscope technology field, and in particular to a kind of fluorescence microscope.
Background technique
Fluorescence microscope is the point light source using a high-luminous-efficiency, is made by the light that color filter array issues certain wavelength
For excitation light source, after exciting the fluorescent material in sample to be tested to launch the fluorescence of various different colours, then pass through object lens and mesh
The amplification of mirror is observed.So in a strong backdrop, it is also very easy to recognize even if fluorescence is very faint, sensibility
Height can be used for the research of eucaryotic cell structure and function, biochemical composition etc..
The excitation light source of traditional fluorescence microscope is the mercury lamp excitation of all-wave length, all in the excitation wavelength range
Fluorescent material is all excited, and when needing to see the excitation fluorescence of some specific fluorescent material, needs through a colour filter, should
Colour filter can only penetrate a kind of fluorescence of wavelength, and then the target by eyepiece observation through colour filter excites fluorescence.
Major defect when mercury lamp is as excitation light source is that all-wave length excites, except the specific fluorescent substance of excitation sample is raw
Outside at specific fluorescent, other fluorescent materials can also be excited in sample to generate our some other unwanted miscellaneous fluorescence；Fluorescence
Through colour filter, colour filter not only weakens or prevents other miscellaneous fluorescence, while also weakening the fluorescence intensity of our needs, and
And the light for passing through colour filter is within the scope of one, the exciting light of fluorescent material similar in such wavelength can also penetrate colour filter,
Just real fluorescence signal is impacted when observation.
In addition, existing fluorescence microscope requires greatly by visually removing observation fluorescence, observation be will lead to for a long time in this way
With asthenopia and discomfort is generated, and then influences the accuracy of observation.
Utility model content
The utility model provides a kind of fluorescence microscope in view of the drawbacks of the prior art.
The purpose of this utility model is achieved through the following technical solutions:
A kind of fluorescence microscope, including light source, object lens, mirror holder, microscope base and objective table, the light source are mounted on the microscope base
Middle part upper end, fixed station and console, the lower removable on the console top and the mirror holder are installed on the microscope base
It is installed together, the top of the mirror holder is provided with movable objective apparatus, and the upper end of the activity objective apparatus is equipped with optical filtering
The upper end of mirror, the optical filtering is equipped with imaging device；The fixed station is mounted on the left side of the console, the fixed station
On be slidably connected objective table.
Further, the upper end of the movable objective apparatus is equipped with eyepiece stalk, and the upper end of the eyepiece stalk is equipped with filter
The upper end of light microscopic, the optical filtering is equipped with imaging device.
Further, the imaging device includes CCD photoreceptor, and the CCD photoreceptor is connected with analog-digital converter, described
Analog-digital converter is connected with computer.
Further, the imaging device is camera, and the camera is connected with computer
Further, the light source is laser light source or LED fluorescent light source.
Further, light source mode switching switch and brightness regulation knob are installed on the side wall of the microscope base.
Further, the optical filtering is interference filters made of multi-layer dielectric film stack.
Further, the lower part of the objective table is equipped with condenser, and the condenser is connected with diaphragm, the optically focused
The light inlet of device is opposite up and down with the light-emitting window of the light source；Coarse adjustment knob and fine tuning knob, institute are installed on the console
It states and is provided with sample propulsion ruler on objective table.
Further, the microscope base around the light source is equipped with more than one reflective mirror swivel mount, each described reflective
Be mounted on reflective mirror on mirror swivel mount, be provided with swivel mount adjusting knob on the mirror holder, the swivel mount adjusting knob with
The number of the reflective mirror swivel mount is corresponding.
Compared with prior art, the utility model at least has the following advantages:
1, fluorescence microscope described in the utility model, the light source generate the exciting light of specific wavelength, the exciting light
Fluorescence sample illumination excitation is generated to the fluorescence of specific wavelength corresponding with the wavelength of the exciting light, the optical filtering
For coming out the fluorescence filter for passing through the specific wavelength that the object lens converge out, the single fluorescence of wavelength is obtained, to improve
The accuracy of imaging, meanwhile, the single fluorescence of the wavelength is imaged by the imaging device, and described image is put by display screen
It shows greatly, naked eyes is avoided to observe the inconvenience of image bring by eyepiece and feel bad.
2, fluorescence microscope described in the utility model adjusts the reflective mirror rotation by the swivel mount adjusting knob
The proper angle of pivoted frame and reflective mirror can will carry out omnidirectional on the laser reflection to sample distributed and to sample,
The sample is further improved to be excited to generate the effect of fluorescence, meanwhile, the fluorescent reflection distributed is also returned object by the reflective mirror
In mirror, the imaging effect of fluorescence is improved.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of fluorescence microscope described in the utility model embodiment 1；
Fig. 2 is the structural schematic diagram of fluorescence microscope described in the utility model embodiment 2；
Fig. 3 is the structural schematic diagram of fluorescence microscope described in the utility model embodiment 3；
Fig. 4 is the structural schematic diagram of fluorescence microscope described in the utility model embodiment 5；
Fig. 5 is the structural schematic diagram of fluorescence microscope described in the utility model embodiment 6；
Fig. 6 is the structural schematic diagram of fluorescence microscope described in the utility model embodiment 7；
1- microscope base；2- imaging device；3- activity objective apparatus；4- object lens；5- mirror holder；6- console；7- objective table；8- is thick
Adjust knob；9- fine tuning knob；10- light source；11- light source mode switching switch；12- brightness regulation knob；13- eyepiece stalk；14- mould
Number converter；15- computer；16- condenser；17- diaphragm；18- reflective mirror swivel mount；19- reflective mirror；20- swivel mount is adjusted
Knob；21- beam expanding lens；22- optical filtering.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples, following embodiment be it is descriptive,
It is not restrictive, protection scope of this utility model cannot be limited by this.
Embodiment 1
As shown in Figure 1, a kind of fluorescence microscope, including light source 10, object lens 4, mirror holder 5, microscope base 1, objective table 7, the light
Source is mounted on the middle part upper end of the microscope base 1, and fixed station and console 6,6 top of console are equipped on the microscope base 1
It is installed together with the lower removable of the mirror holder 5, the top of the mirror holder 5 is provided with movable objective apparatus 3, the live animal
The upper end of lens device 3 is equipped with optical filtering 22, and the optical filtering 22 is interference filters made of multi-layer dielectric film stack
22, the upper end of the optical filtering 22 is equipped with imaging device 2；The fixed station is mounted on the left side of the console 6, described solid
Determining slidable connection on platform has objective table 7；The coarse adjustment knob 8 for adjusting objective focal length and fine tuning rotation are installed on the console 6
Button 9 is provided with sample on the objective table 7 and promotes ruler.The light source generates the exciting light of specific wavelength, and the exciting light is by institute
The fluorescence that fluorescence sample illumination excitation generates specific wavelength corresponding with the wavelength of the exciting light is stated, the optical filtering 22 is used
Come out in by the fluorescence filter for passing through the specific wavelength that the object lens 4 convergence comes out, obtain the single fluorescence of wavelength, with improve at
The accuracy of picture, meanwhile, the single fluorescence of the wavelength is imaged by the imaging device 2, and described image is put by display screen
It shows greatly, naked eyes is avoided to observe the inconvenience of image bring by eyepiece.
The light source is laser light source or LED fluorescent light source, and light source mode switching is equipped on the side wall of the microscope base 1 and is opened
Close 11 and brightness regulation knob 12.The light source mode switching switch 11 is for controlling the laser light source or LED fluorescence light
Source generates specific wavelength and generates switching between different wave length, and the brightness regulation knob 12 is for controlling the laser light
The brightness that source or LED fluorescent light source shine.
The laser for certain wavelength that the light source 10 generates only excites fluorescence sample to generate the laser with certain wavelength
The wavelength of corresponding fluorescence, different fluorescence fluctuates between ± 1nm up and down, can greatly reduce other substances to target substance
It is excited and generates the interference of fluorescence, reduce the generation of miscellaneous fluorescence, thus realize to different fluorescent object qualitative observations, and
To fluorescence qualitative observations different in same sample, the accuracy of imaging is improved
The light source may include 5 kinds of different output modes, under each output mode, the light source output one
The laser of kind wavelength.For example, the laser light source in this example embodiment can provide respectively wavelength be respectively 520nm,
5 kinds in 570nm, 615nm, 565nm, 520nm, 565nm, 667nm, 620nm；But it should be recognized that this example embodiment party
It is not limited thereto in formula, according to actual needs, the light source, which can also correspond to, provides the laser of other wavelength.In practical operation
In, it can according to need by the output mode of toggle lights to provide different wave length laser, and open light source every time only
Radiate the light of single wavelength.The switching of output mode can be by PC controls such as computers 15, can also be by light source
The upper switching that output mode switching part (mode selector switch) is set and realizes output mode.The laser light source 11 can be
Solid State Laser light source 11 (crystal and neodymium glass), gas laser light source 11 (including atom, ion, molecule, quasi-molecule), liquid swash
Radiant 11 (including organic dyestuff, inorganic liquid, chelate) and semiconductor laser light resource 11 etc., the LED fluorescent light source can
Think light emitting diode, does not do particular determination to this in this example embodiment.The laser of different wave length can excite in sample
The fluorescence of specific fluorescent substance generation specific wavelength；For example, the laser of different wave length with it is specific glimmering in its excited sample
It includes following one kind of multiple that stimulative substance, which generates the corresponding relationship between the fluorescence of specific wavelength to may include: 520nm/492nm,
570nm/554nm、615nm/588nm、565nm/555nm、520nm/490nm、565nm/552nm、667nm/650nm、
620nm/596nm；But it should be recognized that be not limited thereto in this example embodiment, according to actual needs, this field skill
Art personnel can also belong to the protection scope of the disclosure with other corresponding relationships of corresponding selection.
Embodiment 2
As shown in Fig. 2, on the basis of embodiment 1, the upper end of the movable objective apparatus 3 is pacified in embodiment 2
Equipped with eyepiece stalk 13, the upper end of the eyepiece stalk 13 is equipped with optical filtering 22, and the upper end of the optical filtering 22 is equipped with imaging dress
Set 2.Compared to embodiment 1, embodiment 2 increases eyepiece, and testing staff can be observed by eyepiece or imaging device 2 simultaneously
Image, convenient for the correction of image.
Embodiment 3
As shown in figure 3, the imaging device 2 preferably includes CCD photoreceptor on the basis of embodiment 1 or embodiment 2,
The CCD photoreceptor is connected with analog-digital converter 14, and the analog-digital converter 14 is connected with computer 15.
Embodiment 4
On the basis of embodiment 1 or embodiment 2, the imaging device 2 is camera, the camera and computer 15
It is connected.
Embodiment 5
As shown in figure 4, the lower part of the objective table 7 is also equipped with condenser 16, described on the basis of embodiment 3 or 4
Condenser 16 is connected with diaphragm 17, and the light inlet of the condenser 16 is opposite up and down with the light-emitting window of the light source 10.It is described
Condenser 16, which is played, converges to the effect that the fluorescence sample substance goes out for the laser that the light source issues, and the diaphragm 17 is used for
The amount of the control convergence light velocity, in order to which the imaging effect observed according to eyepiece adjusts.
Embodiment 6
As shown in figure 5, the microscope base 1 around the laser light source is equipped with more than one anti-on the basis of embodiment 5
Light microscopic swivel mount 18 is mounted on reflective mirror 19 on each reflective mirror swivel mount 18, is provided with swivel mount on the mirror holder 5
Adjusting knob 20, the swivel mount adjusting knob 20 are corresponding with the number of the reflective mirror swivel mount 18.
The suitable angular of the reflective mirror swivel mount 18 and reflective mirror 19 is adjusted by the swivel mount adjusting knob 20
Degree, can will on the laser reflection to sample that distributed and to sample carry out omnidirectional, further improve the sample by
Swash the effect for generating fluorescence, meanwhile, the reflective mirror also returns the fluorescent reflection distributed in object lens, improves the imaging effect of fluorescence
Fruit.
Embodiment 7
As shown in fig. 6, the lower part of the condenser 16 is also fixedly connected with laser beam expanding mould on the basis of embodiment 6
Block, the laser beam expanding module are preferably beam expanding lens 21.The setting of beam expanding lens 21 is in the light source 10 and the condenser 16
Between, for the condenser 16 will to be input to after the laser beam expanding of the light source output；It should be noted that if laser function
Rate density is too low, and the fluorescence signal that cell generates cannot overcome background noise, and image quality is poor, therefore the power after laser beam expanding
Density is more important, and in this example embodiment, the power density after the laser beam expanding can be 30Mw/cm2To 110Mw/
cm2。
More than, the only preferable specific embodiment of the utility model, but the protection scope of utility model is not limited to
This, anyone skilled in the art within the technical scope disclosed by the utility model, the variation that can be readily occurred in
Or replacement, it should be covered within the scope of the utility model.Therefore, the protection scope of the utility model should be with right
Subject to the protection scope of claim.
Claims (9)
1. a kind of fluorescence microscope, including light source, object lens, mirror holder, microscope base and objective table, which is characterized in that the light source installation
In the middle part upper end of the microscope base, fixed station and console, the console top and the mirror holder are installed on the microscope base
Lower removable be installed together, the top of the mirror holder is provided with movable objective apparatus, the upper end of the activity objective apparatus
Optical filtering is installed, the upper end of the optical filtering is equipped with imaging device；The fixed station is mounted on the left side of the console,
Objective table has been slidably connected on the fixed station.
2. fluorescence microscope according to claim 1, which is characterized in that the upper end of the activity objective apparatus is equipped with mesh
Lens barrel, the upper end of the eyepiece stalk are equipped with optical filtering, and the upper end of the optical filtering is equipped with imaging device.
3. fluorescence microscope according to claim 1 or 2, which is characterized in that the imaging device includes CCD photoreceptor,
The CCD photoreceptor is connected with analog-digital converter, and the analog-digital converter is connected with computer.
4. fluorescence microscope according to claim 1 or 2, which is characterized in that the imaging device is camera, described to take the photograph
As head is connected with computer.
5. fluorescence microscope according to claim 4, which is characterized in that the light source is laser light source or LED fluorescence light
Source.
6. fluorescence microscope according to claim 5, which is characterized in that be equipped with light source mode on the side wall of the microscope base
Switching switch and brightness regulation knob.
7. fluorescence microscope according to claim 6, which is characterized in that the optical filtering is multi-layer dielectric film stack
Made of interference filters.
8. fluorescence microscope according to claim 7, which is characterized in that the lower part of the objective table is equipped with condenser,
The condenser is connected with diaphragm, and the light inlet of the condenser is opposite up and down with the light-emitting window of the light source；The control
Coarse adjustment knob and fine tuning knob are installed on platform, sample is provided on the objective table and promotes ruler.
9. fluorescence microscope according to claim 8, which is characterized in that microscope base around the light source be equipped with one with
On reflective mirror swivel mount, be mounted on reflective mirror on each reflective mirror swivel mount, be provided with swivel mount on the mirror holder
Adjusting knob, the swivel mount adjusting knob are corresponding with the number of the reflective mirror swivel mount.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820251492.5U CN208367324U (en) | 2018-02-11 | 2018-02-11 | A kind of fluorescence microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820251492.5U CN208367324U (en) | 2018-02-11 | 2018-02-11 | A kind of fluorescence microscope |
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| Publication Number | Publication Date |
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| CN208367324U true CN208367324U (en) | 2019-01-11 |
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| CN201820251492.5U Active CN208367324U (en) | 2018-02-11 | 2018-02-11 | A kind of fluorescence microscope |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111830696A (en) * | 2020-05-14 | 2020-10-27 | 舟山医院 | Liver cancer cell proliferation observation device |
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2018
- 2018-02-11 CN CN201820251492.5U patent/CN208367324U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111830696A (en) * | 2020-05-14 | 2020-10-27 | 舟山医院 | Liver cancer cell proliferation observation device |
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