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CN208362312U - A kind of red blood cell standing sedimentation separator - Google Patents

A kind of red blood cell standing sedimentation separator Download PDF

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Publication number
CN208362312U
CN208362312U CN201820676832.9U CN201820676832U CN208362312U CN 208362312 U CN208362312 U CN 208362312U CN 201820676832 U CN201820676832 U CN 201820676832U CN 208362312 U CN208362312 U CN 208362312U
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CN
China
Prior art keywords
container
red blood
blood cell
standing sedimentation
sedimentation separator
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201820676832.9U
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Chinese (zh)
Inventor
张雷
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Shenzhen City Cheung Ping Yuan Technology Co Ltd
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Shenzhen City Cheung Ping Yuan Technology Co Ltd
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Priority to CN201820676832.9U priority Critical patent/CN208362312U/en
Application granted granted Critical
Publication of CN208362312U publication Critical patent/CN208362312U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model relates to blood separation field, in particular to a kind of red blood cell standing sedimentation separator.A kind of red blood cell standing sedimentation separator, including container, container are provided with entrance, and the adjacent inner wall parallel with sedimentation direction of container is unsymmetric structure.The utility model is unsymmetric structure by the setting container adjacent inner wall parallel with sedimentation direction, has upset stress balance situation of the whole blood cells in existing cylindrical settling vessel, has improved the sinking speed and separating effect of each ingredient.

Description

A kind of red blood cell standing sedimentation separator
Technical field
The utility model relates to blood separation fields, in particular to a kind of red blood cell standing sedimentation separator.
Background technique
Currently, known is commonly that blood is fitted into test tube or blood bag from the device and method of separating red corpuscle in blood Obtained using gradient centrifugation be added in perhaps blood the sedimentation agents such as hydroxyethyl starch is stood in said vesse obtain or The sedimentation agents such as hydroxyethyl starch are added in blood, and gradient centrifugation obtains again in said vesse.
Separating red corpuscle is to extract the very important step of the blood constituents such as leucocyte, blood platelet, blood plasma from blood Suddenly, if red blood cell removal it is insufficient, centrifugation or time of repose it is too long, the blood platelet and leucocyte for being suspended in top can be into one Step sedimentation, approaches again or sinks to red blood cell layer, and other blood constituent separating difficulties in subsequent step is caused to increase, extraction it is pure It spends, is inefficient, even resulting in separation and Extraction failure.
In view of this, special propose the utility model.
Utility model content
The purpose of this utility model is to provide a kind of red blood cell standing sedimentation separator, the red blood cell standing sedimentations point Separation from device for red blood cell in whole blood increases the speed of red blood cell separation, improves the yield of red blood cell.
In order to realize the above-mentioned purpose of the utility model, the following technical scheme is adopted:
A kind of red blood cell standing sedimentation separator, the red blood cell standing sedimentation separator includes container, the appearance Device is provided with entrance, and the adjacent inner wall parallel with sedimentation direction of the container is unsymmetric structure.
Existing whole blood staticly settles device and generally carries out in cylindrical container, such as the test tube of different size.But Its sinking speed generally per hour 2mm, sinking speed is slow, and the yield after separation is bad.In consideration of it, general using centrifugation Machine accelerated sedimentation, although CENTRIFUGAL ACCELERATING sedimentation, its yield and obtained ingredient purity be still to be improved.In addition, using Centrifuge centrifugation also has certain damage to cell itself, influences subsequent use or testing result, and increase lock out operation Difficulty.
The utility model is unsymmetric structure by the setting container adjacent inner wall parallel with sedimentation direction, has upset whole blood Stress balance situation of the cell in existing cylindrical settling vessel, improves the sinking speed and separating effect of each ingredient. Wherein, it is unsymmetric structure that adjacent inner wall, which is between the unsymmetric structure i.e. adjacent plane of container inner wall, such as with Blood Sedimentation side It is non-disc, such as rectangular, polygon, arc, the asymmetrical section of irregular shape adjacent surface to consistent section.Citing comes Say, the inner wall of container is four faces, between two adjacent faces for the rectangle of different in width or two adjacent faces it Between for different in width trapezoidal etc. or other asymmetric irregular shapes face;The inner wall of the application may be multiple Face, or be the face of certain radian, certainly, the radian of adjacent inner wall is different.
The hydrophobicity of utility model people discovery, each plane of container is different, also influences the sinking speed and separation of cell Degree.
Further, at least side of the container inner wall parallel with sedimentation direction has hydrophily.
Since the ingredient in blood has bigger expansionary force to hydrophilic inner wall, and then have for hydrophobic inner wall There is bigger convergent force, by the way that inner wall to be arranged to different hydrophobicitys, upsets inner wall to the active force of cell, so that The a certain upright position of settling vessel forms a collapsing face, so that red blood cell drops to the bottom of settling vessel faster, and Since the troposphere that reaction force is formed rises leucocyte constantly, increase the speed and purity of the two separation.
Whole blood is added into hydroxyethyl starch stratification relative to existing container, need 60min or so just and can make each layer it Between demarcate obvious, and the yield of isolated ingredient only has 70%-80%;And settling vessel provided by the utility model, it will be complete Blood adds hydroxyethyl starch stratification, it is only necessary to and the time of repose of 10-20min can separate each layer, after separation, The yield of the obtained red blood cell in whole blood is in 85%-98%.
Further, container can be cuboid or gengon, wherein being preferred with cuboid, it is preferable that the container Shape be cuboid.The shape of container is cuboid both processing easy to produce, and reaches separation speed faster when standing Spend and reach higher purity.Specifically, each side length of cuboid, wherein longest side length be most short side long at least 2 times with On.It can be such as 2 times, 3 times, 5 times, 8 times, 10 times, 15 times, not exceed 20 times generally.The size of specific container also needs The volume of the whole blood to separate as needed determines.
Preferably, the bottom surface of the container is additionally provided with the first conduit.In the bottom surface of container, setting conduit is easy for sink The red blood cell dropped in lower vessel portion separates.
In addition, standing sedimentation separator provided by the utility model can be used for separation blood platelet and leucocyte, root According to the position of sedimentation, corresponding site is provided with delivery line.
Since the conduit of container bottoms is when separating red blood cell, equilibrium air pressure is needed in container, the utility model can be with It is balanced, but is more troublesome by the entrance of container, further, the top surface of the container is additionally provided with the second conduit. The conduit primarily serves the effect of equilibrium air pressure, can also be used as the entrance of whole blood to replace the entrance being arranged on container.
In order to allow the red cell fraction sufficiently settled sufficiently to flow out, it is preferable that first conduit is arranged in the container Base angle at.
Wherein, the first conduit and/or the second conduit being arranged in the utility model can be directly connected to external device, into And the input of whole blood and the output operation of red blood cell are carried out, to prevent the whole blood in container to be contaminated.
In the utility model, the material of container is generally transparent or semitransparent plastics, preferably select and blood compatibility compared with Good plastics, the medical polystyrene material used as existing.Its hydrophobic surface can keep its smooth with general technology, Such as polishing, surface siliconization processing.
Container stratification in the utility model, can be horizontal by container bottoms and blood interface, and all keeps horizontal State is stood;Container lean can also be placed.
Verified, container lean is placed, and the bottom surface of container and remaining each face are red so from the horizontal by certain angle Cell settlement direction can form an angle with wherein each inner surface, wherein being with the angle in each face of container lean and horizontal plane Separating effect is good between 18 degree and 60 degree.The wedge shape or conical hopper sample area that red blood cell is formed along container inside wall in infall process Domain is gradually converged, and during contacting and sliding with inner surface of container, is formed effective aggregation, is accelerated erythrocyte sedimentation, The effect of red blood cell separation is improved, shortens the time of erythrocyte sedimentation to greatest extent.
The inclination of the container manually, or can rely on a fixed bracket to assist completing.
Further, the red blood cell standing sedimentation separator further includes bracket, and the container lean is arranged described On bracket.
Container is placed on bracket, and the container bottoms and side Thursday inner surface are protected with blood always during inclined Hold sufficient contact, after container lean, if there is hydrophobic surface, red blood cell settles be contacted with hydrophobic surface side at first, to prevent cell Wall built-up.
Preferably, the axis of the container and the angle of horizontal direction are 18 degree to 60 degree.
Internal stent wedge shape or the angle of taper inner groove surface can be fixed, and can also manually or automatically adjust.Tool Body, angled regulating device can be set in the inside of bracket, such as can be an a telescopic rod either hydraulic strut Deng.
The utility model additionally provides the method using the red blood cell standing sedimentation separator separating red corpuscle, packet Include following steps:
It is mixed hydroxyethyl starch in whole blood input pod, is added, stands 10-20min, red blood cell is removed.
The additive amount of the additive amount of hydroxyethyl starch routinely carries out, and the quality for the hydroxyethyl starch generally added is dense Degree is 5%-6%.
Standing in step can also be placed on quiet with certain gradient on bracket using container is laid flat standing It sets.Pipette removal can be used in red blood cell removal, can also be exported using the delivery line of bottom surface.
In addition, being stood using container provided by the utility model, the time of standing does not exceed 30min, is more than After 30min, floats on leucocyte above and be easy to break through the tunica albuginea layer of layering, into red blood cell layer.
Compared with prior art, the utility model has the following beneficial effects:
(1) the utility model is unsymmetric structure by the setting container adjacent inner wall parallel with sedimentation direction, is upset Stress balance situation of the whole blood cells in existing cylindrical settling vessel improves the sinking speed and separation effect of each ingredient Fruit.
(2) the container provided by the utility model at least side inner wall parallel with sedimentation direction has hydrophily, passes through Inner wall is arranged to different hydrophily and hydrophobicity, upsets inner wall to the active force of cell, so that in settling vessel A certain upright position forms a collapsing face, so that red blood cell drops to the bottom of settling vessel faster, and due to reaction The convection action that power is formed rises leucocyte constantly, increases the speed and purity of the two separation.
(3) container lean provided by the utility model is placed, and the bottom surface of container and remaining each face are from the horizontal by one Clamp angle, the wedge shape or conical hopper sample region that red blood cell is formed along container inside wall in infall process gradually converge, and with During inner surface of container is contacted and slid, effective aggregation is formed, erythrocyte sedimentation is accelerated, improves the effect of red blood cell separation Fruit shortens the time of erythrocyte sedimentation to greatest extent.
(3) red blood cell in whole blood is separated using container provided by the utility model, standing 10-20min obtains red For the yield of cell between 85%-98%, obtained red blood cell purity reaches 95% or more.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the perspective view of container provided by the embodiment of the utility model;
Fig. 2 is the main view of container shown in FIG. 1;
Fig. 3 is the container main view shown in FIG. 1 for filling whole blood;
Fig. 4 is the structural schematic diagram that conduit is arranged in container bottoms provided by the embodiment of the utility model;
Fig. 5 is the structural schematic diagram that conduit is arranged in container bottoms provided by the embodiment of the utility model and top surface;
Fig. 6 is the structural schematic diagram that conduit is arranged in another container bottoms provided by the embodiment of the utility model and top surface;
Fig. 7 is the structural schematic diagram that conduit is arranged in another container bottoms provided by the embodiment of the utility model and top surface;
Fig. 8 is the structural schematic diagram that conduit is arranged in another container bottoms provided by the embodiment of the utility model and top surface;
Fig. 9 is the structural schematic diagram of bracket provided by the embodiment of the utility model;
Figure 10 is that container provided by the embodiment of the utility model is placed on the structural schematic diagram on bracket;
Appended drawing reference:
1- container;2- entrance;3- hydrophilic surface;4- red blood cell layer;The first conduit of 5-;
The second conduit of 6-;7- bracket.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical Novel protected range.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection;It can be mechanical connection, be also possible to be electrically connected;Can be directly connected, can also indirectly connected through an intermediary, It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition The concrete meaning of language in the present invention.
Fig. 1 is the perspective view of container provided by the embodiment of the utility model;Fig. 2 is the main view of container shown in FIG. 1;Such as Shown in Fig. 1-2, red blood cell standing sedimentation separator provided in this embodiment, including container 1, there is any discrepancy for the setting of container 1 Mouth 2, the adjacent inner wall parallel with sedimentation direction of the container 1 are unsymmetric structure.
The utility model is unsymmetric structure by the setting container adjacent inner wall parallel with sedimentation direction, has upset whole blood Stress balance situation of the cell in existing cylindrical settling vessel, improves the sinking speed and separating effect of each ingredient.
Specifically, it is generally carried out in cylindrical container relative to existing stood for whole blood, such as different size Test tube, sedimentation need 1h or so, and the yield after separation is below 80%;And centrifuge accelerated sedimentation is used, to cell itself Also there is certain damage, influence subsequent use or testing result, and increase the difficulty of lock out operation.The utility model provides Red blood cell standing sedimentation separator, sedimentation only need 10-20min, the yield after separation is 85% or more.
Wherein, container adjacent inner wall provided by the utility model is between unsymmetric structure, that is, container inner wall adjacent plane It is such as non-disc, such as rectangular, polygon, arc, irregular shape with the consistent section in Blood Sedimentation direction for unsymmetric structure The asymmetrical section of adjacent surface.For example, the inner wall of container is four faces, is different in width between two adjacent faces Rectangle, for the trapezoidal etc. of different in width or other are right as depicted in figs. 1 and 2 or between two adjacent faces The face of the irregular shape of title;The inner wall of the application may be multiple faces, or be the face of certain radian, certainly, adjacent inner wall Radian it is different.
The hydrophobicity of utility model people discovery, each plane of container is different, also influences the sinking speed and separation of cell Degree.
Further, at least side of the container 1 inner wall parallel with sedimentation direction has hydrophily.Such as Fig. 2 institute The thick line shown is expressed as hydrophilic surface 3, and the line not blackened is then considered as hydrophobic surface.Container at least side and parallel interior in sedimentation direction Wall is hydrophilic surface 3, and by sedimentation telegoniometer, it is hydrophilic surface 3 that container inner wall in parallel, which can have a face, remaining is hydrophobic Face;It can also be hydrophilic surface 3 there are two inner wall, remaining is hydrophobic surface;It can also be hydrophilic surface 3 there are three inner wall, remaining For hydrophobic surface;Etc., be just not listed one by one.
Since the ingredient in blood has bigger expansionary force to hydrophilic inner wall, and then have for hydrophobic inner wall There is bigger convergent force, by the way that inner wall to be arranged to different hydrophily and hydrophobicity, upsets inner wall to the active force of cell, from And to form a collapsing face in a certain upright position of settling vessel, so that red blood cell drops to settling vessel faster Bottom, and since the convection action that reaction force is formed rises leucocyte constantly, increase the speed and purity of the two separation.
Whole blood is added into hydroxyethyl starch stratification relative to existing container, need 60min or so just and can make each layer it Between demarcate obvious, and the yield of isolated ingredient only has 70%-80%;And settling vessel provided by the utility model, it will be complete Blood adds hydroxyethyl starch stratification, it is only necessary to which the time of repose of 10-20min can separate each layer, and separating effect is such as Shown in Fig. 3, after separation, the yield of the obtained red blood cell in whole blood is 85% or more.
Further, container can be cuboid or gengon, wherein being preferred with cuboid, it is preferable that the container Shape be cuboid, as shown in Figure 1.The shape of container is cuboid both processing easy to produce, and reaches more when standing Fast separating rate and reach higher purity.Specifically, each side length of cuboid, wherein longest side length is that most short side is long At least 2 times or more.It can be such as 2 times, 3 times, 5 times, 8 times, 10 times, 15 times, not exceed 20 times generally.Specific container The volume of the whole blood that size also needs to separate as needed determines.
As shown in Figure 4, it is preferable that the bottom surface of the container is additionally provided with the first conduit 6.In the bottom surface of container, conduit is set It is easy for that the red blood cell separation of lower vessel portion will be deposited in.The direction and position of conduit can arbitrarily select.
In addition, standing sedimentation separator provided by the utility model can be used for separation blood platelet and leucocyte, root According to the position of sedimentation, corresponding site is provided with delivery line.
Since the conduit of container bottoms is when separating red blood cell, equilibrium air pressure is needed in container, the utility model can be with It is balanced, but is more troublesome by the entrance of container, further, as viewed in figures 5-8, the top surface of the container is also set up There is the second conduit 5.The conduit primarily serves the effect of equilibrium air pressure, can also be used as the entrance of whole blood and is arranged on container to replace Entrance 2.Second conduit can be set in any position of container top surface, as long as reaching equilibrium air pressure, generally.
In order to allow the red cell fraction sufficiently settled sufficiently to flow out, it is preferable that first conduit is arranged in the container Base angle at, it is specific as shown in Figure 4-8.
Wherein, the first conduit and/or the second conduit being arranged in the utility model can be directly connected to external device, into And the input of whole blood and the output operation of red blood cell are carried out, to prevent the whole blood in container to be contaminated.
In the utility model, the material of container is generally transparent or semitransparent plastics, preferably select and blood compatibility compared with Good plastics, the medical polystyrene material used as existing.Its hydrophobic surface can keep its smooth with general technology, Such as polishing, surface siliconization processing.
Container stratification in the utility model, can be horizontal by container bottoms and blood interface, and all keeps horizontal State is stood;Container lean can also be placed.
Verified, container lean is placed, and the bottom surface of container and remaining each face are red so from the horizontal by certain angle Cell settlement direction can form an angle with wherein each inner surface, wherein being with the angle in each face of container lean and horizontal plane Separating effect is good between 18 degree and 60 degree.The wedge shape or conical hopper sample area that red blood cell is formed along container inside wall in infall process Domain is gradually converged, and during contacting and sliding with inner surface of container, is formed effective aggregation, is accelerated erythrocyte sedimentation, The effect of red blood cell separation is improved, shortens the time of erythrocyte sedimentation to greatest extent.
The inclination of the container manually, or can rely on a fixed bracket 7 to assist completing as shown in Figure 9.Setting Internal stent wedge shape or the angle of taper inner groove surface can be fixed, can also manually or automatically adjust;Specifically, bracket Inside can be set angled regulating device, such as can be an a telescopic rod either hydraulic strut.
Further, the red blood cell standing sedimentation separator further includes for by the bracket 7 of the container lean.
As shown in Figure 10, container is placed on bracket 7, the container bottoms and side Thursday inner surface during inclined, Always it is adequately contacted with blood holding, after container lean, if there is hydrophobic surface, red blood cell settles be contacted with hydrophobic surface one at first Side, to prevent cell wall built-up.
Preferably, the axis of the container and the angle of horizontal direction are 18 degree to 60 degree.
In addition, the container in the utility model after being put into bracket 7, holding by the setting of the first conduit 5 due to the limitation of picture At the base angle of wedge shape or conical hopper sample region that device inner sidewall is formed, do not shown in Figure 10, convenient for adequately by precipitating Red blood cell separation.
The utility model additionally provides the method using the red blood cell standing sedimentation separator separating red corpuscle, packet Include following steps:
It is mixed hydroxyethyl starch in whole blood input pod, is added, stands 10-20min, red blood cell is removed.
The additive amount of the additive amount of hydroxyethyl starch routinely carries out.
Standing in step can also be placed on quiet with certain gradient on bracket 7 using container is laid flat standing It sets.Pipette removal can be used in red blood cell removal, can also be exported using the delivery line of bottom surface.
In addition, being stood using container provided by the utility model, the time of standing does not exceed 30min, is more than After 30min, floats on leucocyte above and be easy to break through the tunica albuginea layer of layering, into red blood cell layer.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited System;Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of Technical characteristic is equivalently replaced;And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new The range of each embodiment technical solution of type.

Claims (10)

1. a kind of red blood cell standing sedimentation separator, which is characterized in that the red blood cell standing sedimentation separator includes holding Device, the container are provided with entrance, and the adjacent inner wall parallel with sedimentation direction of the container is unsymmetric structure.
2. red blood cell standing sedimentation separator according to claim 1, which is characterized in that at least side of the container The inner wall parallel with sedimentation direction has hydrophily.
3. red blood cell standing sedimentation separator according to claim 1, which is characterized in that the container is cuboid knot Structure.
4. red blood cell standing sedimentation separator according to claim 1, which is characterized in that the bottom surface of the container is also set It is equipped with the first conduit.
5. red blood cell standing sedimentation separator according to claim 4, which is characterized in that the top surface of the container is also set It is equipped with the second conduit.
6. red blood cell standing sedimentation separator according to claim 4, which is characterized in that the first conduit setting exists At the base angle of the container.
7. red blood cell standing sedimentation separator according to claim 1, which is characterized in that the material of the container is poly- Styrene.
8. red blood cell standing sedimentation separator according to claim 1, which is characterized in that the red blood cell standing sedimentation Separator further includes bracket, and the container lean setting is on the bracket.
9. red blood cell standing sedimentation separator according to claim 8, which is characterized in that the axis and water of the container Square to angle be 18 degree to 60 degree.
10. red blood cell standing sedimentation separator according to claim 8, which is characterized in that described in the bracket is placed The angle adjustable of container.
CN201820676832.9U 2018-05-08 2018-05-08 A kind of red blood cell standing sedimentation separator Expired - Fee Related CN208362312U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108359582A (en) * 2018-05-08 2018-08-03 深圳市祥平元创科技有限公司 A kind of red blood cell standing sedimentation separator and separation method
WO2021131509A1 (en) * 2019-12-26 2021-07-01 エイブル株式会社 Cell separation method and cell separator
WO2021173481A1 (en) * 2020-02-24 2021-09-02 Cell Code, Llc Use of a polygonal mirrored vessel to irradiate platelets

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108359582A (en) * 2018-05-08 2018-08-03 深圳市祥平元创科技有限公司 A kind of red blood cell standing sedimentation separator and separation method
CN108359582B (en) * 2018-05-08 2024-04-26 深圳市祥平元创科技有限公司 Erythrocyte standing sedimentation separation device and separation method
WO2021131509A1 (en) * 2019-12-26 2021-07-01 エイブル株式会社 Cell separation method and cell separator
WO2021173481A1 (en) * 2020-02-24 2021-09-02 Cell Code, Llc Use of a polygonal mirrored vessel to irradiate platelets

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