CN208076815U - Simple fluorescence microscope - Google Patents

Abstract

The utility model relates to the technical field of microscope production, in particular to a simple fluorescent microscope, comprising four knobs, a first guide post and a second guide post, a first collar and a second collar, a first guide bush and a second guide cover, the first support rod and the second support rod, the first object loading platform and the second object loading platform, the metal mirror plate, the objective lens, the excitation filter, the cut filter and the lighting circuit, the high brightness through the lighting circuit The light of the light-emitting diode passes through the round hole in the middle of the loading platform, and shoots to the objective lens through the excitation filter, sample, and cut-off filter. The observer can clearly observe the fluorescence image of the sample through the objective lens, and at the same time adjust the metal mirror plate The height of the objective lens can be adjusted. The simple fluorescence microscope of the utility model has the advantages of low production cost, high imaging sensitivity, strong detection ability, small size, convenient portability and use, etc., can be mass-produced and widely used in the field of science education.

Description

Simple Fluorescence Microscopy

technical field

The invention relates to the technical field of microscope production, in particular to a simple fluorescence microscope.

Background technique

At present, the weight of the biological microscope is usually 5-6kg, the length of the long mechanical tube with the eyepiece is 16cm, the length of the loading platform is 14-16cm, and the width is 12-14cm. , and consumes a lot of power.

Although professional fluorescence microscopes have good imaging effects, they are expensive and have complex and precise structures. Special attention should be paid to safety when using them. When the power supply of the fluorescent microscope is working, the mercury lamp chamber is extremely hot, and it is necessary to prevent burns and mercury lamp explosion, especially when observing specimens through the ultraviolet baffle, avoid directly observing the focus of the light spot under the objective lens with the naked eye to prevent ultraviolet rays from leaking to the retina of the human eye At the same time, the light sources are ultra-high pressure mercury lamps, ultra-high pressure xenon lamps, high-power halogen lamps, etc., so the power consumption is relatively large, so the above two microscopes are not suitable for wide application in the teaching field.

Contents of the invention

In view of the above problems, the present invention provides a simple fluorescent microscope, through which the light of a high-brightness light-emitting diode passes through the round hole in the middle of the loading platform, passes through the excitation filter, the sample, and the cut-off filter to the objective lens, and the high-brightness light passes through the excitation filter. After the light sheet, only light of a specific wavelength is emitted to excite the sample, so that the sample produces fluorescence of different colors. After passing through the cut-off filter, the fluorescence of these different colors is blocked except for the fluorescence to be observed. Finally, the observer Fluorescent images with high brightness, high resolution and good contrast can be observed through the objective lens. The invention has the advantages of high imaging sensitivity, simple structure without eyepiece, low cost, small volume and easy to carry and use, and effectively solves the above problems.

The technical scheme adopted in the present invention:

A simple fluorescent microscope is characterized in that: it comprises a first object loading platform (1), and the lower end surface of the first object loading platform (1) is welded with a first support rod (3) and a second support rod (4), A second loading platform (2) is welded between the first supporting rod (3) and the second supporting rod (4), and a second circular hole (7) is arranged in the middle of the second loading platform (2) , an excitation filter (8) is covered directly above the second circular hole (7), and an illumination box (5) is welded on the lower end surface of the second loading platform (2), and the illumination box (5) is directly For the second round hole (7), a lighting circuit is arranged in the lighting box (5);

A first circular hole (9) is arranged in the middle of the first loading platform (1), and the first circular hole (9) is located directly above the second circular hole (7). The first loading platform (1) The upper end surface is welded with a first guide sleeve (13) and a second guide sleeve (18), and a cut-off filter (10) is installed between the first guide sleeve (13) and the second guide sleeve (18) ), the first guide sleeve (13) is threaded with the first guide post (16), the first guide post (16) is threaded with the first knob (17), and the first guide post (16) A first collar (14) is arranged on the top, and the first collar (14) is threadedly connected with the third knob (15) and the first collar (14) is fixed on the first knob (15) by the third knob (15). On a guide post (16);

The second guide sleeve (18) is threaded with the second guide post (21), the second guide post (21) is threaded with the second knob (22), and the second guide post (21) is provided with There is a second collar (19), the second collar (19) is threaded with the fourth knob (20) and the second collar (19) is fixed on the second guide by the fourth knob (20). On the column (21), a metal mirror plate (11) is welded between the first collar (14) and the second collar (19), and the middle part of the metal mirror plate (11) is provided with a second Three circular holes (12), the third circular hole (12) is located directly above the first circular hole (9), and an objective lens is placed in the third circular hole (12).

After the emitted light of the high-brightness light-emitting diode of the lighting circuit passes through the second circular hole, the excitation filter, the first circular hole, the sample and the cut-off filter, a fluorescent image of the sample is formed on the objective lens; since the excitation filter contains different The excitation spectrum required by the substance, when the emitted light of the light source passes through the excitation filter, the precipitated spectrum overlaps with the absorption spectrum of the sample to obtain the maximum fluorescence, so the sample produces fluorescence of different colors after passing through the excitation filter, which is convenient Observe and analyze the chemical components and cell components in the sample; the cut-off filter transmits the fluorescence of the specimen to the objective lens for imaging, and at the same time blocks other fluorescence including emitted light, preventing these other fluorescence from participating in imaging, and finally obtains both Fluorescent images with high brightness, high resolution and good contrast that can be observed and photographed.

Further, the first guide post (16) and the second guide post (21) are engraved with a scale of 1mm-5mm on the side.

Turn the third knob and the fourth knob, and after the knobs are loose, you can move the metal mirror plate up and down to adjust the focal length of the objective lens, and the focusing range is 1mm-5mm.

Further, the diameter of the third circular hole (12) is 1.4cm-3.1cm, and the diameters of the first circular hole (9) and the second circular hole (7) are both 1cm-3cm.

The third round hole is used to place the objective lens, and the first round hole and the second round hole let the emitted light of the high-brightness LED pass through.

Further, the distance between the first loading platform (1) and the second loading platform (2) is 3cm-5cm, and the distance between the cut-off filter (10) and the first loading platform (1) is 3cm-8cm, When the lower edge of the first collar (14) or the second collar (19) is located at the guide post scale of 1mm, the distance between the metal mirror plate (11) and the cut-off filter (10) is 3cm-5cm .

Further, the lighting circuit includes a power supply, the anode of the power supply is connected to the anode of the light-emitting diode D1 through the switch K1, the cathode of the light-emitting diode D1 is connected to the negative pole of the power supply, and the light-emitting diode D1 (6) is located in the second round hole Directly below (7); the light-emitting diode D1 (6) is a high-brightness light-emitting diode with a luminous intensity of 10-100mcd, an operating voltage of 2V-3.6V, and an operating current of 0.02A-0.03A.

The power supply is a 3V battery; the high-brightness light-emitting diodes are circular, with diameters of 5mm, 8mm, and 10mm, and the types of light include ultraviolet LEDs, blue LEDs, and green LEDs. Lamps are used as lighting or excitation light sources, and these traditional light sources have the disadvantages of short life, long start-up warm-up time, high energy consumption, and pollutants such as lead and mercury. It meets the light source requirements of fluorescent microscopes with sufficient brightness and uniform illumination area, and avoids the shortcomings of professional fluorescent microscopes such as mercury lamp explosion, damage to eyes by ultra-high-brightness light, and high energy consumption.

Further, the cut-off filter (10) includes an upper wrapping sheet (25) and a lower wrapping sheet (25) stacked up and down, and the lengths of the upper wrapping sheet (25) and the lower wrapping sheet (26) are the same, 2mm larger than the diameter of the third circular hole (12), the first center hole (28) is arranged in the middle of the upper bag sheet (25), and the second center hole (28) is arranged in the middle of the lower bag sheet (26). 29), the upper bag piece (25) and the lower bag piece (26) are superimposed up and down in the shape of "ten", the first central hole (28) and the second central hole (29) correspond up and down, and the first A microlens (27) is clamped between the central hole (28) and the second central hole (29), and the diameters of the first central hole (28) and the second central hole (29) are the same, both of which are microlenses. 2/3-3/4 of (27), the microlens (27) is respectively bonded to the first center hole (28) and the second center hole (29) by an adhesive.

After the microlens is bonded up and down to the first center hole and the second center hole by an adhesive such as Canadian gum coating solution, it is stuck between the upper bag and the lower bag, and the objective lens made in this way is installed on the The metal mirror plate is more solid and not easy to fall.

Further, the length of the first loading platform (1) is 10cm-13cm.

Further, the metal mirror-mounted plate (11) has a thickness of 2mm-4mm and a width of 4cm-5cm.

Further, the length of the first support rod (3) and the second support rod (4) is 10cm-20cm.

In summary, due to the adoption of the above technical solution, the present invention has the following beneficial effects:

The invention is a simple fluorescence microscope, which has the advantages of simple structure without eyepiece, low production cost, high imaging sensitivity, strong detection ability, small size, easy to carry and use, low energy consumption, and can avoid explosion of mercury lamp and ultraviolet leakage to human eyes. It has the advantages of causing damage to the retina, can be mass-produced and widely used in the field of science education, and has the prospect of large-scale promotion and application.

Description of drawings

In order to illustrate the examples of the present invention or the technical solutions in the prior art more clearly, the accompanying drawings required in the description of the implementation examples or the prior art will be briefly introduced below. Obviously, the accompanying drawings in the following description are only the present invention. For some examples of the invention, those skilled in the art can also obtain other drawings based on these drawings without paying any inventive step.

Fig. 1 is a simple fluorescent microscope structure diagram;

Figure 2 is a working structure diagram of a simple fluorescent microscope;

Figure 3 is a side scale view of the first guide post and the second guide post;

Fig. 4 top view of metal mirror plate;

Figure 5 is a top view of the first loading platform;

Fig. 6 the top view of the second loading platform;

Fig. 7 lighting circuit diagram;

Figure 8 top view of the objective lens;

Figure 9 side view of the objective lens;

In the drawings, 1-first loading platform, 2-second loading platform, 3-first support rod, 4-second support rod, 5-lighting box, 6-light-emitting diode, 7-second round hole , 8-excitation filter, 9-first circular hole, 10-cut-off filter, 11-metal mirror plate, 12-third circular hole, 13-first guide sleeve, 14-first collar, 15-the third knob, 16-the first guide post, 17-the first knob, 18-the second guide sleeve, 19-the second collar, 20-the fourth knob, 21-the second guide post, 22-the second Knob, 23-cover glass, 24-slide glass, 25-upper package, 26-lower package, 27-microlens, 28-first center hole, 29-second center hole.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the examples of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example 1:

As shown in Figure 1, a simple fluorescence microscope includes a first loading platform, the lower end surface of the first loading platform is welded with a first support rod and a second support rod, and the first support rod and the second support A second loading platform is welded between the rods, a second circular hole is arranged in the middle of the second loading platform, and an excitation filter is covered directly above the second circular hole, and the bottom of the second loading platform is The end face is welded with a lighting box, the lighting box is facing the second round hole, and a lighting circuit is arranged in the lighting box; a first round hole is set in the middle of the first loading platform, and the first round hole is located at the Directly above the second round hole, a first guide sleeve and a second guide sleeve are welded on the upper end surface of the first loading platform, and a cut-off filter is installed between the first guide sleeve and the second guide sleeve , the first guide sleeve is threaded with the first guide post, the first guide post is threaded with the first knob, the first guide post is provided with a first collar, and the first collar is connected with the first knob The three knobs are threaded and the third knob fixes the first collar on the first guide post, the second guide sleeve is threaded with the second guide post, and the second guide post is threaded with the second knob , the second guide post is provided with a second collar, the second collar is threadedly connected with the fourth knob and the second collar is fixed on the second guide post by the fourth knob, the first A metal mirror plate is welded between the one collar and the second collar, and a third round hole is arranged in the middle of the metal mirror plate, and the third round hole is located directly above the first round hole, An objective lens is placed in the third circular hole;

As shown in Figure 3, the scale of 1mm-5mm is engraved on the side of the first guide post and the second guide post; as shown in Figure 4, the diameter of the third circular hole is 1.4cm-3.1cm, as shown in Figure 5 and As shown in Figure 6, the diameters of the first circular hole and the second circular hole are both 1cm-3cm;

As shown in Figure 1, the distance between the first loading platform and the second loading platform is 3cm-5cm, the distance between the cut-off filter and the first loading platform is 3cm-8cm, and the first collar or the second When the lower edge of the collar is located at the guide post scale 1mm, the distance between the metal mirror plate and the cut-off filter is 3cm-5cm;

As shown in Figure 7, the lighting circuit includes a power supply, the anode of the power supply is connected to the anode of the light-emitting diode D1 through the switch K1, the cathode of the light-emitting diode D1 is connected to the negative pole of the power supply, and the light-emitting diode D1 is located in the second round hole Directly below, the LED D1 is a high-brightness LED with a luminous intensity of 10-100mcd, an operating voltage of 2V-3.6V, and an operating current of 0.02A-0.03A.

As shown in Figures 8 and 9, the cut-off filter includes an upper wrapper and a lower wrapper stacked up and down, the lengths of the upper wrapper and the lower wrapper are the same, and they are all smaller than the third hole diameter 2mm larger, a first central hole is provided in the middle of the upper wrapping sheet, a second central hole is arranged in the middle of the lower wrapping sheet, the upper wrapping sheet and the lower wrapping sheet are stacked up and down in the shape of "ten", the first The central hole and the second central hole correspond up and down, and a microlens is sandwiched between the first central hole and the second central hole, and the diameters of the first central hole and the second central hole are the same, both of which are 2/3-3/4, the microlens is respectively bonded to the first center hole and the second center hole through an adhesive;

As shown in Figure 1, the length of the first object-carrying platform is 10cm-13cm; the thickness of the metal mirror plate is 2mm-4mm, and the width is 4cm-5cm; the length of the first support rod and the second support rod It is 10cm-20cm.

Fluorescence is divided into autofluorescence and secondary fluorescence. Autofluorescence means that the specimen can produce fluorescence when it is irradiated by the excitation light of the light source without any treatment. This example is an autofluorescence experiment. The specimen is chlorophyll, the light source is an ultraviolet light-emitting diode, and an excitation filter is an ultraviolet excitation filter, and the cut-off filter is a red cut-off filter, as shown in Figure 2, place the slide on the first round hole of the first loading platform, and then place the specimen on the slide , and finally cover with a coverslip. Press the switch button K1, when the high-brightness circular ultraviolet light-emitting diode emits ultraviolet light, the ultraviolet excitation filter only allows ultraviolet light with a center wavelength of 365nm and a half-peak width of 50nm to pass through, and the chlorophyll of the specimen is irradiated by ultraviolet light and spontaneously emits When the red fluorescence is emitted, the red cut-off filter blocks other light and only allows the red fluorescence emitted by the specimen to pass through for imaging. At this time, the operator can observe the fluorescence image of chlorophyll through the microlens.

Example 2:

As shown in Figure 1, a simple fluorescence microscope includes a first loading platform, the lower end surface of the first loading platform is welded with a first support rod and a second support rod, and the first support rod and the second support A second loading platform is welded between the rods, a second circular hole is arranged in the middle of the second loading platform, and an excitation filter is covered directly above the second circular hole, and the bottom of the second loading platform is The end face is welded with a lighting box, the lighting box is facing the second round hole, and a lighting circuit is arranged in the lighting box; a first round hole is set in the middle of the first loading platform, and the first round hole is located at the Directly above the second round hole, a first guide sleeve and a second guide sleeve are welded on the upper end surface of the first loading platform, and a cut-off filter is installed between the first guide sleeve and the second guide sleeve , the first guide sleeve is threaded with the first guide post, the first guide post is threaded with the first knob, the first guide post is provided with a first collar, and the first collar is connected with the first knob The three knobs are threaded and the third knob fixes the first collar on the first guide post, the second guide sleeve is threaded with the second guide post, and the second guide post is threaded with the second knob , the second guide post is provided with a second collar, the second collar is threadedly connected with the fourth knob and the second collar is fixed on the second guide post by the fourth knob, the first A metal mirror plate is welded between the one collar and the second collar, and a third round hole is arranged in the middle of the metal mirror plate, and the third round hole is located directly above the first round hole, An objective lens is placed in the third circular hole;

As shown in Figure 3, the scale of 1mm-5mm is engraved on the side of the first guide post and the second guide post; as shown in Figure 4, the diameter of the third circular hole is 1.4cm-3.1cm, as shown in Figure 5 and As shown in Figure 6, the diameters of the first circular hole and the second circular hole are both 1cm-3cm;

As shown in Figure 1, the distance between the first loading platform and the second loading platform is 3cm-5cm, the distance between the cut-off filter and the first loading platform is 3cm-8cm, and the first collar or the second When the lower edge of the collar is located at the guide post scale 1mm, the distance between the metal mirror plate and the cut-off filter is 3cm-5cm;

As shown in Figure 7, the lighting circuit includes a power supply, the anode of the power supply is connected to the anode of the light-emitting diode D1 through the switch K1, the cathode of the light-emitting diode D1 is connected to the negative pole of the power supply, and the light-emitting diode D1 is located in the second round hole Directly below, the LED D1 is a high-brightness LED with a luminous intensity of 10-100mcd, an operating voltage of 2V-3.6V, and an operating current of 0.02A-0.03A.

As shown in Figures 8 and 9, the cut-off filter includes an upper wrapper and a lower wrapper stacked up and down, the lengths of the upper wrapper and the lower wrapper are the same, and they are all smaller than the third hole diameter 2mm larger, a first central hole is provided in the middle of the upper wrapping sheet, a second central hole is arranged in the middle of the lower wrapping sheet, the upper wrapping sheet and the lower wrapping sheet are stacked up and down in the shape of "ten", the first The central hole and the second central hole correspond up and down, and a microlens is sandwiched between the first central hole and the second central hole, and the diameters of the first central hole and the second central hole are the same, both of which are 2/3-3/4, the microlens is respectively bonded to the first central hole and the second central hole through an adhesive;

As shown in Figure 1, the length of the first object-carrying platform is 10cm-13cm; the thickness of the metal mirror plate is 2mm-4mm, and the width is 4cm-5cm; the length of the first support rod and the second support rod It is 10cm-20cm.

Fluorescence is divided into autofluorescence and secondary fluorescence. Secondary fluorescence means that the specimen must be chemically treated, such as staining the specimen with fluorescent pigments, so that the specimen can emit fluorescence under the action of excitation light, and then the operator can get the color from the fluorescence microscope. Different cells and tissues of the specimen can be clearly distinguished from the contrast. The present embodiment is a secondary fluorescence experiment, the specimen is a wheat stem vascular bundle, the light source is an ultraviolet light-emitting diode, the excitation filter is an ultraviolet excitation filter, and the cut-off filter is a blue cut-off filter, using the fluorescent pigment safranin - Fast Green double-stained wheat stem vascular bundles, then as shown in Figure 2, put the slide on the first round hole of the first loading platform, then put the specimen on the slide, and finally put the lid on slides. Press the switch button K1, when the high-brightness circular ultraviolet light-emitting diode emits ultraviolet light, the ultraviolet excitation filter only allows ultraviolet light with a center wavelength of 365nm and a half-peak width of 50nm to pass through. , the xylem of the vascular bundle and the sheath of the vascular bundle emit blue fluorescence, and the blue cut-off filter blocks other light and only allows the blue fluorescence emitted by the specimen to pass through for imaging. At this time, the operator can observe the wheat stem through the microlens Fluorescence image of bundle tissue.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection of the invention. within range.

Claims (9)

1. A simple and easy fluorescent microscope, characterized in that: comprise the first loading platform (1), the first supporting rod (3) and the second supporting rod (4) are welded on the lower end surface of the first loading platform (1) ), a second loading platform (2) is welded between the first support bar (3) and the second support bar (4), and a second round hole (2) is provided in the middle of the second loading platform (2) ( 7), the said second circular hole (7) is covered with an excitation filter (8), the lower end surface of said second loading platform (2) is welded with a lighting box (5), the lighting box (5 ) is facing the second round hole (7), and a lighting circuit is arranged in the lighting box (5); A first circular hole (9) is arranged in the middle of the first loading platform (1), and the first circular hole (9) is located directly above the second circular hole (7). The first loading platform (1) The first guide sleeve (13) and the second guide sleeve (18) are welded on the upper end surface, and a cut-off filter (10) is installed between the first guide sleeve (13) and the second guide sleeve (18) ), the first guide sleeve (13) is threaded with the first guide post (16), the first guide post (16) is threaded with the first knob (17), and the first guide post (16) A first collar (14) is arranged on the top, and the first collar (14) is threadedly connected with the third knob (15) and the first collar (14) is fixed on the first knob (15) by the third knob (15). On a guide post (16); The second guide sleeve (18) is threaded with the second guide post (21), the second guide post (21) is threaded with the second knob (22), and the second guide post (21) is provided with There is a second collar (19), the second collar (19) is threaded with the fourth knob (20) and the second collar (19) is fixed on the second guide by the fourth knob (20). On the column (21), a metal mirror plate (11) is welded between the first collar (14) and the second collar (19), and the middle part of the metal mirror plate (11) is provided with a second Three round holes (12), the third round hole (12) is located directly above the first round hole (9), and an objective lens is placed in the third round hole (12). 2. A simple fluorescent microscope according to claim 1, characterized in that: the first guide post (16) and the second guide post (21) are engraved with a scale of 1mm-5mm on the side. 3. A simple fluorescent microscope according to claim 1, characterized in that: the third round hole (12) has a diameter of 1.4cm-3.1cm, and the first round hole (9) and the second round hole (7) have a diameter of 1cm-3cm. 4. A kind of simple and easy fluorescent microscope according to claim 1, is characterized in that: described first loading platform (1) is 3cm-5cm apart from second loading platform (2), and described cut-off filter ( 10) 3cm-8cm away from the first loading platform (1), when the lower edge of the first collar (14) or the second collar (19) is located at the guide column scale 1mm, the metal mirror plate ( 11) 3cm-5cm away from the cut-off filter (10). 5. A simple fluorescent microscope according to claim 1, characterized in that: the lighting circuit includes a power supply, the anode of the power supply is connected to the anode of the light-emitting diode D1 through the switch K1, and the cathode of the light-emitting diode D1 is connected to the negative pole of the power supply , the light emitting diode D1 (6) is located directly below the second circular hole (7); The light-emitting diode D1 (6) is a high-brightness light-emitting diode with a luminous intensity of 10-100mcd, an operating voltage of 2V-3.6V, and an operating current of 0.02A-0.03A. 6. A simple fluorescence microscope according to claim 1, characterized in that: the cut-off filter (10) comprises an upper wrapper (25) and a lower wrapper (26) stacked up and down, the upper wrapper (26) The length of wrapping sheet (25) and following wrapping sheet (26) is the same, all bigger than described the 3rd round hole (12) diameter 2mm, first central hole (28) is arranged in the middle of described last wrapping sheet (25), A second central hole (29) is arranged in the middle of the lower wrapper (26), the upper wrapper (25) and the lower wrapper (26) are stacked up and down in the shape of a "ten", and the first center hole (28 ) and the second center hole (29) correspond up and down, a microlens (27) is sandwiched between the first center hole (28) and the second center hole (29), the first center hole (28) and the second center hole (29) The diameters of the two central holes (29) are the same, which are 2/3-3/4 of the microlens (27), and the microlens (27) is connected to the first central hole (28), the first central hole (28) and the second microlens respectively by an adhesive. Two central holes (29) are bonded. 7. A simple fluorescent microscope according to claim 1, characterized in that: the length of the first loading platform (1) is 10cm-13cm. 8. A simple fluorescence microscope according to claim 1, characterized in that: the metal mirror plate (11) has a thickness of 2mm-4mm and a width of 4cm-5cm. 9. A simple fluorescent microscope according to claim 1, characterized in that: the length of the first support rod (3) and the second support rod (4) is 10cm-20cm.