CN208044181U - A kind of automatically scanning inverted fluorescence microscope mechanical-optical setup and light channel structure - Google Patents
A kind of automatically scanning inverted fluorescence microscope mechanical-optical setup and light channel structure Download PDFInfo
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- CN208044181U CN208044181U CN201820500437.5U CN201820500437U CN208044181U CN 208044181 U CN208044181 U CN 208044181U CN 201820500437 U CN201820500437 U CN 201820500437U CN 208044181 U CN208044181 U CN 208044181U
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- fluorescence microscope
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- support plate
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Abstract
The utility model provides a kind of automatically scanning inverted fluorescence microscope mechanical-optical setup and light channel structure, wherein automatically scanning inverted fluorescence microscope mechanical-optical setup includes support plate, support plate is equipped with electricity driving displacement platform, electricity driving displacement platform is equipped with sample box, side is equipped with upper bracket in support plate, and upper bracket is equipped with bright field illumination system；Fluorescence objective apparatus and lower bracket are respectively equipped with below support plate, lower bracket is equipped with electric rotary body, electric rotary body is equipped with the colour filter group turntable with cut-off colour filter, the side of lower bracket is equipped with fluorescent lighting system close to the position of the colour filter group turntable, and fluorescent lighting system is connected with LED fluorescence integrated optical sources；The same side of lower bracket is additionally provided with CCD adaptation lens devices, and one end that CCD is adapted to lens device has the pedestal for tilting 45 °, reflective mirror is fixed on the pedestal；The other end of CCD adaptation lens devices is connected with automatic focusing system, and ccd sensor is connected on the automatic focusing system.This case greatly improves the working efficiency of fluorescence microscope, extends its application range.
Description
Technical field
The utility model is related to fluorescence microscope fields, and in particular to a kind of automatically scanning inverted fluorescence microscope ray machine
And light channel structure.
Background technology
Fluorescence microscope is the point light source using a high-luminous-efficiency, is generally adopted by mercury lamp or LED as glimmering
Radiant, the light that certain wavelength is sent out after color filter array are allowed to emit as the fluorescent material in exciting light, excitation sample
After going out the fluorescence of various different colours, then carry out by the amplification of micro objective and eyepiece the shape and structure of observation sample.
Under the strong scape to backing, easy identification fluorescence is very faint, sensibility is high, be mainly used for eucaryotic cell structure and function and
The research of chemical composition etc..The effect of fluorescence microscope light source is not direct illumination, but as glimmering in a kind of excitation sample
The energy of stimulative substance.Why we can observe sample, be because fluorescent material is presented after absorbing the luminous energy excited in sample
Fluorescence phenomenon.
Fluorescence microscope, which is broadly divided into, just sets fluorescence microscope and inverted fluorescence microscope, is primarily adapted for use in without type
Sample.Currently common fluorescence microscope, which is substantially, to be needed to be manually operated, manual focusing, manual switching colour filter and light source,
Searching and mobile sample areas manually, manual analyzing data etc., not only checkability is very low, and the sample that can not carry out batch exists
Line is observed, and the accuracy and consistency examined can also be influenced by human factor.Meanwhile existing fluorescence microscope by
It is limited in by fluorescence objective angular field size, when using fluorescence microscopy and shooting image, can only once shoot very little
Region, it is larger or a fairly large number of sample, general fluorescence microscope cannot be met the requirements for that size.
Utility model content
One purpose of the utility model is in view of the drawbacks of the prior art, to provide a kind of special automatically scanning inversion fluorescence
Microscope machine and light channel structure, to improve the working efficiency of fluorescence microscope.
Particularly, the utility model provides a kind of automatically scanning inverted fluorescence microscope mechanical-optical setup, including support
Plate, the support plate are equipped with electricity driving displacement platform, and the electricity driving displacement platform is equipped with sample box, and side is equipped in the support plate
Upper bracket, the upper bracket are equipped with bright field illumination system；Fluorescence objective apparatus is respectively equipped with below the support plate under
Holder, the lower bracket are equipped with electric rotary body, and the electric rotary body is equipped with the colour filter with cut-off colour filter
The side of piece group turntable, the lower bracket is equipped with fluorescent lighting system, the fluorescence close to the position of the colour filter group turntable
Lighting system is connected with LED fluorescence integrated optical sources；The same side of the lower bracket is additionally provided with CCD adaptation lens devices, and the CCD is suitable
One end of glasses-selecting equipment has the pedestal for tilting 45 °, and reflective mirror is fixed on the pedestal；The CCD is adapted to the another of lens device
One end is connected with automatic focusing system, and ccd sensor is connected on the automatic focusing system.
Further, the sample box is located at the middle position of the electricity driving displacement platform.
Further, the sample box is porous structure.
Further, the electricity driving displacement platform is in X-direction, the moveable electricity driving displacement platform of Y-direction.
Further, the bright field illumination system includes white LED light source.
Further, the fluorescence objective apparatus is located at below the support plate at the position of middle.
Further, the bright field illumination system is located at the surface of the sample box.
This case additionally provides a kind of automatically scanning inverted fluorescence microscope light channel structure, including：By LED fluorescence Integrated Lights
Source, fluorescent lighting system, cut-off colour filter, fluorescence objective apparatus, the transmitting light path that biological cell sample is constituted, and by biology
The optical system for testing of cell sample, fluorescence objective apparatus, cut-off colour filter, reflective mirror, CCD adaptive mirrors, ccd sensor composition,
In, the transmitting light path utilizes the cut-off colour filter using the reflective formation for ending colour filter, the optical system for testing
Light transmission is formed.
Further, further include the bright field illumination system being set to right over the biological cell sample.
Further, the biological cell sample is set in a sample box with porous structure.
This case uses full automatic sample mobile platform, sample box to be set on electricity driving displacement platform, set on electric rotary body
There is the colour filter group turntable with cut-off colour filter, and using automatic focusing system etc., and integration is subject to it, finally
Realize inverted fluorescence microscope automatic scanning, can to the biological cell sample of large scale or big quantity carry out automatically scanning at
Picture greatly improves the working efficiency of fluorescence microscope, extends its application range.
According to the accompanying drawings to the detailed description of the utility model specific embodiment, those skilled in the art will be more
Add the above-mentioned and other purposes, advantages and features of clear the utility model.
Description of the drawings
Some specific realities of the utility model are described in detail by way of example rather than limitation with reference to the accompanying drawings hereinafter
Apply example.Identical reference numeral denotes same or similar component or part in attached drawing.It should be appreciated by those skilled in the art that
The drawings are not necessarily drawn to scale.In attached drawing：
Fig. 1 is the schematic diagram of the automatically scanning inverted fluorescence microscope mechanical-optical setup of one embodiment of the utility model；
Fig. 2 is another signal of the automatically scanning inverted fluorescence microscope mechanical-optical setup of one embodiment of the utility model
Figure；
Fig. 3 is the schematic diagram of the automatically scanning inverted fluorescence microscope light channel structure of one embodiment of the utility model.
Specific implementation mode
As shown in Figs. 1-2, present embodiment discloses a kind of automatically scanning inverted fluorescence microscope mechanical-optical setups, including branch
Fagging 1, the support plate 1 are equipped with electricity driving displacement platform 2, and the electricity driving displacement platform 2 is equipped with sample box 5, in the support plate 1
Side is equipped with upper bracket 3, and the upper bracket 3 is equipped with bright field illumination system 4；It is respectively equipped with fluorescence below the support plate 1
Objective apparatus 6 and lower bracket 7, the lower bracket 7 are equipped with electric rotary body 8, and the electric rotary body 8, which is equipped with, to be had
End the colour filter group turntable 9 of colour filter 17, the side of the lower bracket 7 is equipped with close to the position of the colour filter group turntable 9
Fluorescent lighting system 10, the fluorescent lighting system 10 are connected with LED fluorescence integrated optical source 11；The same side of the lower bracket 7
CCD adaptation lens devices 12 are additionally provided with, one end of the CCD adaptations lens device 12 has the pedestal 16 for tilting 45 °, the pedestal 16
On be fixed with reflective mirror 13；The other end of the CCD adaptations lens device 12 is connected with automatic focusing system 14, the automatic focusing
Ccd sensor 15 is connected in system 14.It is with respect to the horizontal plane or vertical plane to tilt 45 °.
This case uses full automatic sample mobile platform, and sample box 5 is set on electricity driving displacement platform 2, on electric rotary body 8
Equipped with the colour filter group turntable 9 with cut-off colour filter 17, and using automatic focusing system 14 etc., and collection is integrated to it
At the final automatic scanning for realizing inverted fluorescence microscope can carry out certainly the biological cell sample of large scale or big quantity
Dynamic scanning imagery, greatly improves the working efficiency of fluorescence microscope, extends its application range.
Preferably, sample box 5 is located at the middle position of the electricity driving displacement platform 2.Bright field illumination system 4 is located at institute
State the surface of sample box 5.Sample box 5 is porous structure.Sample box 5 is porous structure, for placing biological cell sample, with
Ensure that light can be irradiated on biological cell sample, sends out the fluorescence of different colours again by excitation.
Electricity driving displacement platform 2 is in X-direction, the moveable electricity driving displacement platform of Y-direction.The electricity driving displacement platform 2 controls simultaneously
The movement of sample and position selection.
Bright field illumination system 4 includes white LED light source.
Fluorescence objective apparatus 6 is located at below the support plate 1 at the position of middle, and position is reliably reasonable.
This case additionally provides a kind of automatically scanning inverted fluorescence microscope light channel structure, and Fig. 2 is above-mentioned light channel structure
Schematic diagram, including, by LED fluorescence integrated optical source 11, fluorescent lighting system 10, cut-off colour filter 17, fluorescence objective apparatus 6 is raw
The transmitting light path that object cell sample is constituted, and by biological cell sample, fluorescence objective apparatus 6, cut-off colour filter 17, reflective mirror
13, the optical system for testing that CCD adaptive mirrors 12, ccd sensor 15 form, wherein the transmitting light path utilizes the cut-off colour filter
17 reflective formation, the optical system for testing are formed using the light transmission of the cut-off colour filter 17.
The operation principle of light channel structure is：The fluorescence that LED fluorescence integrated optical source 11 is sent out by fluorescent lighting system 10 into
Line convergence then by the excitation colour filter 17 in the colour filter group turntable 9, then is reflected into the fluorescence objective apparatus 6,
It finally beats on the biological cell sample in sample box 5.Biological cell sample sends out the fluorescence of different colours by excitation again, then
It is amplified by fluorescence objective apparatus 6, is then transmitted by the cut-off colour filter 17 in the colour filter group turntable 9,
After being reflected using the reflective mirror 13, by entering the ccd sensor 15 after the CCD adaptive mirrors 12, eventually by CCD
Software shows in the form of images, it is ensured that test result is precisely, reliably.
Further include the bright field illumination system 4 being set to right over the biological cell sample, there can be better effect.
Biological cell sample is set in a sample box 5 with porous structure, to ensure that it is biological thin that light can be irradiated to
On born of the same parents' sample, the fluorescence of different colours is sent out again by excitation.
So far, although those skilled in the art will appreciate that the more of the utility model have been shown and described in detail herein
A exemplary embodiment still, still can be according to the utility model public affairs in the case where not departing from the spirit and scope of the utility model
The content opened directly determines or derives many other variations or modifications for meeting the utility model principle.Therefore, this practicality is new
The range of type is understood that and regards as to cover other all these variations or modifications.
Claims (10)
1. a kind of automatically scanning inverted fluorescence microscope mechanical-optical setup, which is characterized in that including support plate, in the support plate
Equipped with electricity driving displacement platform, the electricity driving displacement platform is equipped with sample box, and side is equipped with upper bracket, the upper branch in the support plate
Frame is equipped with bright field illumination system；Fluorescence objective apparatus and lower bracket, the lower bracket are respectively equipped with below the support plate
It is equipped with electric rotary body, the electric rotary body is equipped with the colour filter group turntable with cut-off colour filter, under described
The side of holder is equipped with fluorescent lighting system close to the position of the colour filter group turntable, and the fluorescent lighting system is connected with
LED fluorescence integrated optical sources；The same side of the lower bracket is additionally provided with CCD adaptation lens devices, one end of the CCD adaptations lens device
With the pedestal for tilting 45 °, reflective mirror is fixed on the pedestal；The other end of the CCD adaptations lens device is connected with automatically
Focusing system is connected with ccd sensor on the automatic focusing system.
2. automatically scanning inverted fluorescence microscope mechanical-optical setup as described in claim 1, it is characterised in that：The sample box
At the middle position of the electricity driving displacement platform.
3. automatically scanning inverted fluorescence microscope mechanical-optical setup as claimed in claim 2, it is characterised in that：The sample box
It is porous structure.
4. automatically scanning inverted fluorescence microscope mechanical-optical setup as claimed in claim 3, it is characterised in that：The electronic position
Moving stage is in X-direction, the moveable electricity driving displacement platform of Y-direction.
5. automatically scanning inverted fluorescence microscope mechanical-optical setup as described in claim 1, it is characterised in that：The light field shines
Bright system includes white LED light source.
6. automatically scanning inverted fluorescence microscope mechanical-optical setup as described in claim 1, it is characterised in that：The fluorescence
Lens device is located at below the support plate at the position of middle.
7. automatically scanning inverted fluorescence microscope mechanical-optical setup as described in claim 1, it is characterised in that：The light field shines
Bright system is located at the surface of the sample box.
8. a kind of automatically scanning inverted fluorescence microscope light channel structure, which is characterized in that including：By LED fluorescence integrated optical source,
Fluorescent lighting system, cut-off colour filter, fluorescence objective apparatus, the transmitting light path that biological cell sample is constituted, and by biological thin
The optical system for testing of born of the same parents' sample, fluorescence objective apparatus, cut-off colour filter, reflective mirror, CCD adaptive mirrors, ccd sensor composition, wherein
The transmitting light path utilizes the light transmission for ending colour filter using the reflective formation for ending colour filter, the optical system for testing
It is formed.
9. automatically scanning inverted fluorescence microscope light channel structure as claimed in claim 8, it is characterised in that：Further include being set to
Bright field illumination system right over the biological cell sample.
10. automatically scanning inverted fluorescence microscope light channel structure as claimed in claim 9, it is characterised in that：The biology
Cell sample is set in a sample box with porous structure.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820500437.5U CN208044181U (en) | 2018-04-10 | 2018-04-10 | A kind of automatically scanning inverted fluorescence microscope mechanical-optical setup and light channel structure |
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| CN201820500437.5U CN208044181U (en) | 2018-04-10 | 2018-04-10 | A kind of automatically scanning inverted fluorescence microscope mechanical-optical setup and light channel structure |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110057724A (en) * | 2019-05-10 | 2019-07-26 | 中国科学院苏州生物医学工程技术研究所 | Small fluorescent is inverted micro imaging system |
| CN110262030A (en) * | 2019-07-05 | 2019-09-20 | 中国医科大学附属盛京医院 | A kind of microscope |
| CN110501797A (en) * | 2019-09-20 | 2019-11-26 | 合肥英睿系统技术有限公司 | A kind of infrared lens device that can detect identification visual field automatically |
| CN114948205A (en) * | 2021-10-30 | 2022-08-30 | 河北医科大学第二医院 | Intraoperative fluorescence navigation microscope for surgery |
-
2018
- 2018-04-10 CN CN201820500437.5U patent/CN208044181U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110057724A (en) * | 2019-05-10 | 2019-07-26 | 中国科学院苏州生物医学工程技术研究所 | Small fluorescent is inverted micro imaging system |
| CN110262030A (en) * | 2019-07-05 | 2019-09-20 | 中国医科大学附属盛京医院 | A kind of microscope |
| CN110501797A (en) * | 2019-09-20 | 2019-11-26 | 合肥英睿系统技术有限公司 | A kind of infrared lens device that can detect identification visual field automatically |
| CN114948205A (en) * | 2021-10-30 | 2022-08-30 | 河北医科大学第二医院 | Intraoperative fluorescence navigation microscope for surgery |
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