CN207937470U - Type 1 duck hepatitis virus antibody detection test strip - Google Patents

Abstract

The utility model 1 type duck hepatitis virus antibody Test paper of one kind comprising：Strip bottom plate, the long side outer rim of upper surface upwardly extend to form two side plates；The upper surface of strip bottom plate is divided into adsorbent chamber, test chamber, control chamber along its axis and holds chamber by deflector successively, adsorbent chamber respectively with test chamber and compare chamber and be connected to, and test chamber with to compare chamber mutual indepedent；Sample absorption layer；Detection trace line is printed on detecting pad, detection trace line is the 1 type duck hepatitis virus antigen liquid printing using purifying；Control trace line is printed on control pad, control trace line is to be printed using sheep or 1 type duck hepatitis virus IgG solution of rabbit-anti；It is padded with holding.The Test paper of 1 type duck hepatitis virus antibody provided by the utility model, can realize and fast and accurately detect, and is easy to operate, testing cost is low, the sample for entering detection zone and control zone is realized into shunting, can be contaminated to avoid the string of the two so that result is more accurate.

Description

Type 1 duck hepatitis virus antibody detection test strip

technical field

The utility model belongs to the field of detection test papers, in particular to a type 1 duck hepatitis virus antibody detection test paper.

Background technique

Type 1 duck hepatitis virus (duck hepatitis virus, DHV) can cause a highly fatal infectious disease in ducklings, characterized by acute onset, rapid transmission, and high mortality. The mortality rate of ducklings within one week of age can reach 95%. Clinical manifestations were opisthotonos, and the pathological changes were hepatitis and hemorrhage. The popular duck hepatitis virus in my country is type 1 duck hepatitis virus (so without special instructions, the duck hepatitis virus in this patent refers to type 1 duck hepatitis virus in particular), and there are duck virus viruses in most provinces, cities and regions of my country. The occurrence and prevalence of hepatitis have caused important economic losses to duck farms.

At present, the methods for detecting type 1 duck hepatitis virus antibody mainly include agar immunodiffusion test (AGP), serum neutralization test, IFA and ELISA. However, AGP takes a long time, and the serum neutralization experiment, IFA and ELISA detection techniques are complicated to operate, require expensive equipment, and the process is long, time-consuming and laborious. It requires specific equipment and professional technicians to operate, and it is difficult to popularize at the grassroots level. Meet the rapid detection needs of the production line or the epidemic site. Therefore, there is an urgent need for a kind of equipment and technology for rapid detection of type 1 duck hepatitis virus antibody.

Utility model content

One purpose of the utility model is to provide a type 1 duck hepatitis virus antibody detection test paper, which can realize fast and accurate detection, and is easy to operate and low in detection cost.

Another purpose of the utility model is to provide a type 1 duck hepatitis virus antibody detection test paper, which can separate the samples entering the detection area and the control area, so as to avoid the cross-contamination of the two and make the result more accurate.

The technical scheme of the utility model is:

Type 1 duck hepatitis virus antibody detection test strip, which includes:

A strip bottom plate, one end of its upper surface has a downward slope, and the outer edge of the long side of the upper surface extends upwards to form two side plates;

A deflector, which is vertically pasted on the upper surface of the strip bottom plate, and divides the upper surface of the strip bottom plate along its axis into an adsorption cavity, a detection cavity, a control cavity and a holding cavity, the adsorption The cavities communicate with the detection cavity and the control cavity respectively, and the detection cavity and the control cavity are independent of each other;

A sample adsorption pad, which is pasted on the strip bottom plate and located in the adsorption chamber, the lower surface of the sample adsorption pad has an inclined boss matching the downward slope;

A detection pad, which is pasted on the strip bottom plate and located in the detection chamber, and the detection pad is printed with a detection imprint line, and the detection imprint line is printed with purified type 1 duck hepatitis virus antigen solution ;

A control pad, which is pasted on the strip bottom plate and positioned in the control cavity, and the control pad is printed with a control imprinted line, and the contrast imprinted line adopts sheep or rabbit anti-type 1 duck hepatitis virus IgG solution print;

and a holding pad, which is pasted on the strip-shaped bottom plate and located in the holding cavity, and the holding pad is folded down to cover the strip-shaped bottom plate.

Preferably, in the type 1 duck hepatitis virus antibody detection test paper,

The deflector includes a first deflector, a second deflector and a third deflector arranged in parallel at intervals along the axis of the strip bottom plate;

The first deflector includes a first conduction section located in its middle section and a second conduction section located at both ends of the first conduction section;

The second deflector includes a blocking section located in the middle section and a third conducting section located at both ends of the blocking section, and the length of the blocking section is equal to that of the first conducting section;

Both ends of the third deflector are respectively connected to the side plates, and the third deflector, the second deflector and the two side plates define the control cavity;

Two partitions parallel to the axis of the strip bottom plate are arranged between the first deflector and the second deflector, and the two partitions are connected with the first conduction section and the The blocking section defines the detection chamber, and the two partitions respectively define two sample channels with the two side plates, the second conduction section and the third conduction section, and the sample A channel communicates with the control chamber.

Preferably, in the type 1 duck hepatitis virus antibody detection test paper,

The sample adsorption pad comprises the first bamboo charcoal fiber layer positioned at the bottom layer, the polyester fiber layer positioned in the middle and the second bamboo charcoal fiber layer positioned at the top layer;

The detection pad comprises a glass wool layer and a carboxymethyl cellulose layer positioned on the glass wool layer;

The control pad has the same structure as the detection pad;

The grip pad is made of water-absorbing material.

Preferably, in the type 1 duck hepatitis virus antibody detection test paper,

The strip bottom plate comprises a substrate made of cardboard strips and a plastic film positioned below the substrate;

The bottom surface of the substrate having one end of the holding pad is recessed upwards to form a holding pad accommodating cavity.

Preferably, in the type 1 duck hepatitis virus antibody detection test paper, the deflector is covered with a plastic protective film, and the positions corresponding to the detection cavity and the control cavity on the plastic protective film have respectively A transparent observation window.

Preferably, in the type 1 duck hepatitis virus antibody detection test paper, the upper surface of the downward slope is a trapezoidal structure, the bottom edge of the trapezoidal structure is the edge of the upper surface of the strip bottom plate, and the trapezoidal The top edge of the structure coincides with the first conducting segment.

Preferably, in the type 1 duck hepatitis virus antibody detection test paper, the angle between the downward slope and the bottom surface of the strip bottom plate is 3°-8°.

The utility model at least has the following beneficial effects:

1. The type 1 duck hepatitis virus antibody detection test paper provided by the utility model does not need to attach any other instruments and reagents when used, as long as the test end is inserted into the sample liquid to be tested for about 30 seconds, and the test result can be judged in about 5 minutes. Simple, fast and accurate operation;

2. The type 1 duck hepatitis virus antibody detection test paper provided by the utility model uses brown-red detection blot lines and control blot lines as positive and negative blots for detection, namely two brown-red blots are displayed, indicating that there is presence in the tested sample. The virus antibody was detected, and the result was positive; only a brown-red control blot C was displayed on the top, indicating that no virus antibody was detected in the tested sample solution, and the result was negative. The result judgment is intuitive, accurate, simple and clear, and is not prone to false negative and false positive misjudgments;

3. When the type 1 duck hepatitis virus antibody detection test paper of the present invention is used, it does not need to be equipped with other instruments, equipment and reagents, which saves a lot of equipment, equipment and additional reagent costs; professional and non-professionals can carry out on-site testing anytime, anywhere For testing, there is no need to pay for expert diagnostic examination fees or travel expenses for sending samples to the diagnostic room, saving testing costs and low testing costs;

4. The type 1 duck hepatitis virus antibody detection test paper provided by the utility model is simple to operate, and is a "one-step" or "fool-style", and is convenient to carry and store, and can meet the needs of personnel at different levels, including professional testing, customs quarantine, Sanitation and epidemic prevention, quality monitoring, animal product processing, intensive breeding to individual breeding, etc., have broad market prospects and good economic and social benefits;

5. The type 1 duck hepatitis virus antibody detection test paper of the present utility model, according to the basic principle of ELISA, detects virus antibody with immunochromatography test paper, utilizes the diafiltration concentration and capillary action of microporous filter membrane, antigen-antibody reaction is by ELISA The traditional liquid-phase environment is transferred to the solid-phase filter membrane for rapid processing, and colloidal gold blotting is used instead of enzyme blotting. The color development of colloidal gold can be directly observed with the naked eye, and the detection result can be obtained immediately. Therefore, this method is more accurate than serological methods such as ELISA. For simplicity and speed.

Other advantages, objectives and features of the utility model will partly be embodied through the following description, and partly will be understood by those skilled in the art through the research and practice of the utility model.

Description of drawings

Fig. 1 is the sectional structure schematic diagram of an embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model;

Fig. 2 is the structural representation of an embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model;

Fig. 3 is a structural schematic diagram of the plastic protective film in an embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the present invention.

Detailed ways

The utility model will be described in further detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it by referring to the description.

It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

As shown in Figure 1, the utility model provides a kind of type 1 duck hepatitis virus antibody detection test paper, and it comprises:

Strip bottom plate 1, one end of its upper surface has a downward slope 101, and the long side outer edge of the upper surface extends upwards to form two side plates 102;

deflector 2, which is vertically pasted on the upper surface of the strip bottom plate 1, and divides the upper surface of the strip bottom plate 1 into an adsorption chamber, a detection chamber, a contrast chamber and a holding chamber sequentially along its axis, The adsorption chamber is respectively communicated with the detection chamber and the control chamber, and the detection chamber and the control chamber are independent of each other;

A sample adsorption pad 3, which is pasted on the strip bottom plate 1 and located in the adsorption chamber 3, the lower surface of the sample adsorption pad has an inclined boss matching the downward slope 101;

A detection pad 4, which is pasted on the strip bottom plate 1 and located in the detection cavity, and the detection pad 4 is printed with a detection imprint line, and the detection imprint line is the purified type 1 duck hepatitis virus Antigen solution printing;

A control pad 5, which is pasted on the strip bottom 1 plate and located in the control chamber, and the control pad 5 is printed with a control imprinted line, and the contrast imprinted line is obtained by using sheep or rabbit anti-type 1 Duck hepatitis virus IgG solution printing;

And a grip pad 6, which is pasted on the strip bottom plate 1 and located in the grip cavity, and the grip pad 6 is folded down to cover the strip bottom plate 1.

The detection area and the control detection area are separated from each other by providing the deflector 2 , and the samples are respectively entered into it for detection, so that the detection accuracy can be improved.

The operating process of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model is as follows:

Aseptically take the blood of the tested animal, separate the serum, and dilute the serum 1:200 times with normal saline to obtain the sample to be tested;

Insert the end of the test paper with the adsorption pad into the sample to be tested, the insertion depth does not exceed the imprinted line, take out the test strip after about 30 seconds, place it horizontally for about 1-5 minutes, and observe the result at the same time.

Result judgment:

If only one brown-red control blot C is displayed, it means that the test result is negative, indicating that no type 1 duck hepatitis virus antibody was detected in the tested sample fluid;

If brown-red control blot C and detection blot T appear, it means that the test result is positive, that is, the type 1 duck hepatitis virus antibody is detected in the sample to be tested;

If no brownish-red blots appear, the test strip has expired or has been mishandled.

As shown in Figure 2, in order to better separate the detection cavity and the control cavity, in an embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model,

The deflector 2 includes a first deflector 201 , a second deflector 202 and a third deflector 203 arranged in parallel at intervals along the axis of the strip bottom plate 1 ;

The first deflector 201 includes a first conduction section located in its middle section and a second conduction section located at both ends of the first conduction section;

The second deflector 202 includes a blocking section located in the middle section and a third conducting section located at both ends of the blocking section, and the length of the blocking section is equal to that of the first conducting section;

Both ends of the third deflector 203 are respectively connected to the side plates 102, and the third deflector 203, the second deflector 202 and the two side plates 102 define the contrast Cavity;

Between the first deflector 201 and the second deflector 202, two partitions 7 parallel to the axis of the strip bottom plate 1 are arranged, and the two partitions 7 are connected to the first deflector. A conduction section and the blocking section define the detection chamber, and the two partitions 7 define two sides with the two side plates 102 and the second conduction section and the third conduction section respectively. A sample channel, the sample channel communicates with the control chamber.

In order to enable each region to realize its function more, its material is individually designed, in one embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model,

The sample adsorption pad 2 comprises the first bamboo charcoal fiber layer positioned at the bottom layer, the polyester fiber layer positioned in the middle and the second bamboo charcoal fiber layer positioned at the top layer;

The detection pad 4 comprises a glass wool layer and a carboxymethyl cellulose layer positioned on the glass wool layer;

The control pad 5 has the same structure as the detection pad 4;

The grip pad 6 is made of water-absorbing material.

In one embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model, the strip bottom plate 1 comprises a substrate made of cardboard strips and a plastic film positioned below the substrate;

The bottom surface of the substrate with one end of the holding pad 6 is recessed upwards to form a holding pad accommodation cavity.

As shown in Figure 3, in order to protect the whole test paper structure and facilitate observation, in an embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model, the deflector 2 is covered with a plastic protective film 8, so There are transparent observation windows 801 on the plastic protective film 8 corresponding to the positions of the detection cavity and the control cavity.

In one embodiment of the type 1 duck hepatitis virus antibody detection test paper provided by the utility model, the upper surface of the downward slope 101 is a trapezoidal structure, and the bottom edge of the trapezoidal structure is the upper surface of the strip bottom plate 1 edge, the top edge of the trapezoidal structure coincides with the first conduction section, and the angle between the downward slope and the bottom surface of the strip bottom plate is 3 °-8 °, which can improve the sample absorption rate.

Although the embodiment of the present utility model has been disclosed as above, it is not limited to the use listed in the description and the implementation, and it can be applied to various fields suitable for the present utility model. For those familiar with the art, Further modifications can be readily effected, so the invention is not limited to the specific details and examples shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims (7)

1. type duck hepatitis virus antibody Test paper, which is characterized in that including：

One end of strip bottom plate, upper surface has a down slop portion, and the long side outer rim of the upper surface upwardly extends shape At two side plates；

Deflector is pasted onto the upper surface of the strip bottom plate vertically, and by the upper surface of the strip bottom plate along its axis Be divided into adsorbent chamber, test chamber, control chamber successively and hold chamber, the adsorbent chamber respectively with the test chamber and described compare chamber Connection, and the test chamber with described to compare chamber mutual indepedent；

Sample absorption layer is pasted onto on the strip bottom plate and is located in the adsorbent chamber, the following table of the sample absorption layer Face has the obliquity prominence platform that a down slop portion matches；

Detecting pad is pasted onto on the strip bottom plate and is located in the test chamber, and is printed with detection on the detecting pad Trace line, the detection trace line are the 1 type duck hepatitis virus antigen liquid printing using purifying；

Control pad is pasted onto on the strip bottom plate and is located at the control intracavitary, and is printed with control on control pad Trace line, the control trace line are to be printed using sheep or 1 type duck hepatitis virus IgG solution of rabbit-anti；

It is padded with holding, is pasted onto on the strip bottom plate and is located at the gripping intracavitary, and the gripping is padded after being turned over downwards Coat the strip bottom plate.

2. 1 type duck hepatitis virus antibody Test paper as described in claim 1, which is characterized in that

The deflector includes the first deflector, the second water conservancy diversion along the axis spaced and parallel setting successively of the strip bottom plate Plate and third deflector；

First deflector includes being located therein the first conduction period of section and being led positioned at the second of first conduction period both ends Logical section；

Second deflector includes the blocking section for being located therein section and the third conduction period positioned at the blocking section both ends, described The equal length of blocking section and first conduction period；

The both ends of the third deflector are connect with the side plate respectively, and the third deflector and second deflector with And two side plates limit the control chamber；

Two parallel with the axis of strip bottom plate are provided between first deflector and second deflector Partition board, two partition boards limit the test chamber, and two partition boards point with first conduction period and the blocking section Two sample channels are not limited with two side plates and second conduction period and the third conduction period, the sample is logical Road compares chamber connection with described.

3. 1 type duck hepatitis virus antibody Test paper as claimed in claim 2, which is characterized in that

The sample absorption layer includes positioned at the first bamboo of bottom, positioned at intermediate layer of polyester fiber and positioned at top layer The second bamboo be made；

The detecting pad includes mineral wool layer and the carboxymethyl cellulose layer on the mineral wool layer；

The control pad is identical as the detection mat structure；

The gripping pad is made of water-absorbent material.

4. 1 type duck hepatitis virus antibody Test paper as claimed in claim 3, which is characterized in that

The strip bottom plate includes using substrate and the plastic film below the substrate made of cardboard item；

There is the bottom surface of described one end for holding pad to be recessed upwards on the substrate and form a gripping pad accommodating chamber.

5. 1 type duck hepatitis virus antibody Test paper as described in claim 1, which is characterized in that the deflector is covered with One layer of plastic protective film for plastics, the position that the test chamber and the control chamber are corresponded on the plastic protective film for plastics are respectively provided with one thoroughly Bright observation window.

6. 1 type duck hepatitis virus antibody Test paper as claimed in claim 2, which is characterized in that in the down slop portion Surface is trapezium structure, and the bottom edge of the trapezium structure is the top surface edge of the strip bottom plate, the top of the trapezium structure Side is overlapped with first conduction period.

7. 1 type duck hepatitis virus antibody Test paper as described in claim 1, which is characterized in that the down slop portion with Angle between the bottom surface of the strip bottom plate is 3 ° -8 °.