CN207891341U - A kind of leukemia of children separation of lymphocytes extraction element - Google Patents

Abstract

A device for separating and extracting lymphocytes from childhood leukemia, comprising an outer sleeve and an inner tube, the inner sleeve is placed inside the outer sleeve and is threadedly connected with the outer sleeve, the outer sleeve is composed of a hollow outer tube body and a sleeve cap, The center of the outer casing has a through outlet of the inner tube. The inner tube includes a hollow inner tube body, a pipe plug and a sealing ring. The bottom of the inner tube body is conical, and an injection hole is opened in the center of the cone. The bottom is provided with limiting fins, and there is a sealing ring groove between the limiting fins, and the sealing ring is sleeved in the sealing ring groove of the inner tube body. The top of the plug cap is securely attached with a finger loop. The device can quickly add blood samples to prevent it from mixing with the separation liquid. After centrifugation, the target liquid can be quickly poured into a new centrifuge tube. At the same time, the reliability of the tube body is improved. The volume chamber of the separation liquid can be adjusted to prevent it from overflowing into the In the inner tube, and can be placed vertically, the operation is more convenient.

Description

A device for separating and extracting lymphocytes from childhood leukemia

technical field

The utility model relates to the field of medical inspection equipment, in particular to a device for separating and extracting children's leukemia lymphocytes.

Background technique

Peripheral blood mononuclear cells (PBMCs) mainly refer to lymphocytes, which are the most commonly used cell populations in immunological experiments. Monocytes are commonly used in basic and clinical in vitro research. How to quickly and effectively isolate monocytes with higher purity from peripheral blood has always been a problem worth exploring. The traditional method of separating mononuclear cells is the Ficoll density gradient separation method, but the difficulty of density gradient stratification lies in: the sample is added too fast during the centrifugation process, the centrifuge tube is unbalanced or the stratification caused by opening the brake is not clear, which will affect the experimental results , and the separation process is cumbersome and takes too long.

In order to solve the above problems, the utility model with the application number 201420466315.0 has designed a cell separation tube for separating and extracting PBMC, which includes a tube cover and a tube body. The middle and lower parts of the tube body are coaxially sealed with an isolation layer, and the isolation layer is an opening at the top. The hollow funnel shape, the center of the bottom of the isolation layer is coaxially provided with a liquid injection hole, which can quickly add the blood sample to the liquid surface of the separation liquid, and prevent the mixing of the blood layer and the separation liquid. During centrifugation, there is no need Turn off the brake of the centrifuge; after centrifugation, the separated PBMCs can be directly poured into a new centrifuge tube easily, which saves time and is easy to operate. It realizes the convenient, fast and efficient extraction to PBMC in a short time, and simplifies the operation steps. However, this device has the following defects: one, the problem that the single-layer tube body collides in the centrifugation process and the bottle body is easily broken; The volume of the cavity cannot be changed. When the amount of separation liquid is too much, it will overflow the top of the isolation layer, making it difficult to quickly add blood samples; 3. The bottom of the tube is conical, making it difficult to place the tube vertically on the operating platform , when the tube body is skewed, the layers in the tube body are mixed, making it difficult to extract PBMCs with ideal purity.

Utility model content

In order to solve the above problems, the utility model designs a device for separating and extracting children's leukemia lymphocytes. There is no need to turn off the brake of the centrifuge; after centrifugation, the separated PBMCs can be directly poured into a new centrifuge tube, which saves time and is easy to operate, and realizes the convenient, fast and efficient extraction of PBMCs in a short time. The double-layer structure design of the device enhances the reliability of the tube body and prevents accidental rupture caused by the collision of the tube body during centrifugation. The cavity containing the separation liquid between the bottom of the inner tube and the bottom of the outer tube can be adjusted to prevent the separation liquid from overflowing into the The inner tube makes it difficult to quickly add blood samples, further saving time, and the bottom of the device increases the contact area of the operating platform, which can be placed vertically on the operating platform, making the operation more convenient.

In order to achieve the above-mentioned technical purpose and achieve the above-mentioned technical effect, the utility model is achieved through the following technical solutions:

A device for separating and extracting lymphocytes from childhood leukemia, characterized in that it includes an outer tube and an inner tube, the inner tube is placed inside the outer tube and is threadedly connected with the outer tube, and the outer tube is composed of a hollow outer tube body and a sleeve The inner wall of the upper section of the outer casing is provided with internal threads, the casing cap is connected to the top of the outer casing, and the center of the outer casing has a through outlet of the inner pipe. The inner pipe includes a hollow inner body, a pipe plug and Sealing ring, the bottom of the inner tube body is conical, the center of the cone is provided with an injection hole, the outer wall of the upper section of the inner tube body is provided with external threads, the bottom of the inner tube body is provided with limiting fins, and the space between the limiting fins is sealed The ring clamping groove, the sealing ring is sleeved in the sealing ring clamping groove of the inner pipe body, the pipe plug is composed of a plug body and a limiting plug cap, and the top of the limiting plug cap is fixedly connected with a finger ring.

Further, the outer sleeve is provided with a measuring scale.

Further, the diameter of the injection hole is 1.1-5mm.

Further, the outer casing and the inner pipe are made of glass, and the sealing ring is made of rubber.

The beneficial effects of the utility model are: the device can not only quickly add the blood sample to the liquid surface of the separation liquid, but also prevent the blood layer from mixing with the separation liquid. During centrifugation, there is no need to close the brake of the centrifuge; after centrifugation, it can directly Easily pour the separated PBMCs into a new centrifuge tube, which is time-saving and easy to operate, and realizes the convenient, fast and efficient extraction to PBMCs in a short time. At the same time, the double-layer structure design of the device enhances the reliability of the tube body. To prevent accidental rupture caused by the collision of the tube body during centrifugation, the cavity containing the separation liquid between the bottom of the inner tube and the bottom of the outer tube can be adjusted to prevent the separation liquid from overflowing into the inner tube, which makes it difficult to quickly add blood samples The purpose is to further save time, and the bottom of the device increases the contact area of the operating platform, and can be placed vertically on the operating platform, making the operation more convenient.

Description of drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the accompanying drawings required for the description of the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those skilled in the art, other drawings can also be obtained based on these drawings without creative effort.

1 is a schematic diagram of the overall structure of a device for separating and extracting lymphocytes from childhood leukemia;

Fig. 2 is the structural representation of described outer casing;

Fig. 3 is the structural representation of described inner tube;

Fig. 4 is a schematic cross-sectional structural view of the inner tube.

In the accompanying drawings, the list of parts represented by each label is as follows:

1-outer casing, 11-casing cap, 101-internal thread, 111-inner pipe outlet; 2-inner pipe, 21-pipe plug, 22-conical bottom, 23-sealing ring, 201-limiting fin, 202-external thread, 203-sealing ring slot, 211-finger buckle, 212-limiting plug cap, 221-injection hole.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without creative efforts belong to the scope of protection of the present utility model.

As shown in Figures 1-4, a device for separating and extracting lymphocytes from childhood leukemia includes an outer tube 1 and an inner tube 2. The outer tube 1 is provided with a measuring scale, and the inner tube 2 is placed inside the outer tube 1 and connected to the outer tube 1. The pipe 1 is threaded, and the outer casing 1 is composed of a hollow outer body and a sleeve cap 11. The upper inner wall of the outer casing 1 is provided with an internal thread, and the casing cap 11 is covered on the top of the outer casing 1. The outer casing 1 There is a through inner tube outlet 111 in the center, and the inner tube 2 includes a hollow inner tube body, a pipe plug 21 and a sealing ring. The bottom of the inner tube body is conical, and an injection hole 221 is opened at the center of the cone. The aperture of the hole 221 is 1.1-5mm, the outer wall of the upper section of the inner pipe body is provided with an external thread 202, and the bottom of the inner pipe body 2 is provided with a limit fin 201, and between the limit fins 201 is a sealing ring clamping groove 203, sealing The ring 23 is sleeved in the sealing ring groove 203 of the inner tube body. The tube plug 21 is composed of a plug body and a stopper cap 212 . The top of the stopper cap 212 is fixedly connected with a finger ring 211 .

When using this device, use a pipette to draw a certain amount of separation liquid and inject the separation liquid into the outer sleeve 1 along the middle and lower sections of the outer sleeve 1, and then cover the sleeve cap 11; Put the outlet 111 of the inner tube into the inner cavity of the outer tube 1, put the plug 21 on the inner tube 2, put the index finger on the ring buckle 211 and continue screwing down into the inner tube 2 until the tapered bottom 22 of the inner tube The lowest point is close to the liquid level of the separation liquid, pull out the plug 21, use a pipette to suck the blood sample and inject the blood sample into the inner tube along the tube wall of the inner tube 2, because the tapered bottom 22 separates the separation liquid Come, the blood sample can be quickly injected without causing the blood sample to mix with the separation liquid; after the blood sample is injected, remove the pipette and cover the plug 21, and place the device on a centrifuge for centrifugation; after the separation, Pull off the tube plug 21, then pour the PBMC (lymphocyte) and supernatant into a new separation tube for the next step of centrifugation. Due to the blocking effect of the conical bottom 22, it can prevent binuclear cells at the bottom when pouring. layer overflow, which saves the time of using a pipette to extract lymphocytes and prevents the bottom liquid layer from being sucked by mistake; during the above operation, when too much separation liquid is injected or more separation liquid needs to be injected, the index finger can be inserted into the finger. The clasp 211 is lifted and rotated, so that the cavity between the conical bottom 22 and the bottom of the outer sleeve 1 is increased to hold more separation liquid, preventing the separation liquid from overflowing from the injection hole 221 of the conical bottom 22 into the inner cavity. In the tube 2; when injecting the separation liquid in the first step, the inner tube 2 can also be placed in a suitable position in the inner cavity of the outer tube 1, and then use a pipette to draw a certain amount of separation liquid and extend it to the bottom of the inner tube. The injection hole 221 injects the separation liquid into the cavity between the inner tube 2 and the bottom of the outer tube 1; since the bottom of the inner tube 2 is provided with a sealing ring 23, it can prevent air from entering the gap between the inner tube and the outer tube. Contaminate the reagent, and the sealing ring 23 can prevent the separation liquid from overflowing from the gap during centrifugation.

In the description of this specification, descriptions referring to the terms "one embodiment", "example", "specific example" and the like mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in the description of the present invention. In at least one embodiment or example. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example.

The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not exhaust all details, nor do they limit the utility model to the specific implementations described. Obviously, many modifications and variations can be made based on the contents of this specification. The invention is to be limited only by the claims and their full scope and equivalents.

Claims (4)

1. A device for separating and extracting lymphocytes from childhood leukemia, characterized in that: it comprises an outer sleeve and an inner tube, the inner tube is placed in the inside of the outer sleeve and is threadedly connected with the outer sleeve, and the outer sleeve is composed of a hollow outer body and an inner tube. The casing cap is composed of an inner thread on the inner wall of the upper section of the outer casing, and the casing cap is connected to the top of the outer casing. Plug and sealing ring, the bottom of the inner tube body is conical, the center of the cone has an injection hole, the outer wall of the upper section of the inner tube body is provided with external threads, and the bottom of the inner tube body is provided with limit fins, between the limit fins It is a sealing ring clamping groove, and the sealing ring is sleeved in the sealing ring clamping groove of the inner pipe body. The pipe plug is composed of a plug body and a limiting plug cap, and the top of the limiting plug cap is fixedly connected with a finger ring. 2. A device for separating and extracting lymphocytes from childhood leukemia according to claim 1, characterized in that: said outer tube is provided with a measuring scale. 3. A device for separating and extracting lymphocytes from childhood leukemia according to claim 1, characterized in that: the diameter of the injection hole is 1.1-5 mm. 4. A device for separating and extracting lymphocytes from childhood leukemia according to claim 1, characterized in that: the outer tube and the inner tube are made of glass, and the sealing ring is made of rubber.