CN207585989U - A kind of automatic staining device - Google Patents
A kind of automatic staining device Download PDFInfo
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- CN207585989U CN207585989U CN201721632307.9U CN201721632307U CN207585989U CN 207585989 U CN207585989 U CN 207585989U CN 201721632307 U CN201721632307 U CN 201721632307U CN 207585989 U CN207585989 U CN 207585989U
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- Prior art keywords
- drop
- automatic staining
- dye liquor
- staining device
- dye
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- 238000010186 staining Methods 0.000 title claims abstract description 32
- 238000004043 dyeing Methods 0.000 claims abstract description 37
- 239000011521 glass Substances 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims description 51
- 239000007788 liquid Substances 0.000 claims description 43
- 239000000523 sample Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 5
- 239000002699 waste material Substances 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims 1
- 238000004040 coloring Methods 0.000 abstract description 7
- 239000000243 solution Substances 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005086 pumping Methods 0.000 description 2
- 238000007592 spray painting technique Methods 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 208000037805 labour Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a kind of automatic staining device, includes the microcontroller of housing, the drop dyeing equipment being installed in housing and control drop dyeing equipment operation, and the drop dyeing equipment includes, and the drop that drop dye processing is carried out to sample contaminates component；The eccentric unit of centrifugal treating is carried out to sample, the turntable for placing glass slide is provided in the upper end of eccentric unit；The drippage that the drop dye component precisely, is measured dye liquor by monolithic function is on the sample of glass slide.Automatic staining device eliminates the inconsistency of manual operation, changes the characteristics of coloring present in existing automatic staining instrument is bad, dye liquor is unevenly distributed with that will dye automation.
Description
" technical field "
The utility model is related to biochemistry colouring arts, and in particular to the automatic staining device of simulating hand dyeing.
" background technology "
It is dyed in hematology, microbiology dyeing, cytological stains, cytology dyeing, reproductive medicine colouring art is usual
It is dyed using artificially colored or instrument, but having the following problems at this stage：Existing hand dyeing method is operated because of different people
Inconsistency is generated, and occupies a large amount of labours；The coloring of existing automatic staining device is bad or reagent dosage is larger；It is existing
There is automatic centrifugation dyeing instrument dye liquor to cover uneven；Existing automatic staining instrument hot air type heating effect is not good enough；It is existing it is centrifugal from
There may be the pollution problems of aerosol in dynamic dyeing instrument centrifugal process；Change existing dyeing instrument and operate more complicated, buying maintenance
The shortcomings that cost is higher；8. changing existing automatic staining instrument spray-painting for a long time can plug-hole phenomenon.
" utility model content "
The utility model is intended to provide one kind and can automate dyeing, eliminates the inconsistency of manual operation, changes existing
Have that the coloring present in automatic staining instrument is bad, dye liquor automatic staining device unevenly distributed.
The technical solution adopted in the utility model is as follows：A kind of automatic staining device, including housing, is installed in housing
It drips dyeing equipment and the microcontroller of control drop dyeing equipment operation, the drop dyeing equipment includes, the drop of drop dye processing is carried out to sample
Contaminate component；The eccentric unit of centrifugal treating is carried out to sample, is provided with to place turning for glass slide in the upper end of eccentric unit
Disk；The drippage that the drop dye component precisely, is measured dye liquor by monolithic function is on the sample of glass slide；
The drop dye component includes, supporting rack；It is installed on the guide post of supporting rack upper end；It is installed on the screw of supporting rack side
Motor；It is installed on the sliding block of supporting rack opposite side, the output terminal connection of the sliding block and lead screw motor and is sheathed on guide post, institute
Sliding block is stated to move along the length direction of guide post by lead screw motor；The water dropper fixed bin of dropping liquid processing, institute are carried out to glass slide
Water dropper fixed bin is stated to be fixed on sliding block, it can be separate by water dropper fixed bin described in sliding block or close to turntable.
It is larger to solve the spray-painting plug-hole phenomenon of existing automatic staining instrument, reagent dosage by drop dye component and eccentric unit
The problem of, dye mode is dripped by change, reduces reagent dosage, and not plug-hole.
Further, the eccentric unit includes, electric machine support and support for rotary disc；The driver being installed on support for rotary disc
Structure；The stepper motor being installed on electric machine support, the output terminal of the stepper motor are connected by synchronous belt and transmission mechanism transmission
It connects；Shaft, the shaft are rotatablely connected with transmission mechanism；Turntable, the turntable are installed on the upper end of shaft, on the periphery of turntable
The uniform slide slot being provided with for placing glass slide.
By eccentric unit function, it is not dry quickly to solve the problems, such as that hand dyeing slide takes off liquid.
Further, the automatic staining device further includes the liquid sensing element for placing color solution bottle, the liquid
Sensing element is installed on housing exterior walls both sides, and the liquid sensing element can detect dye liquor surplus in color solution bottle automatically.
Further, the drop dye component is two；By the pump and liquid line being installed on housing, the water dropper is consolidated
Determine box to connect with color solution bottle.
Further, the automatic staining device further includes the heating element for being heated to dye liquor；It is described
Heating element include stack gradually arrangement first heating cover board, the first heating plate, the first heating cavity, the second heating cavity,
Second heating plate and the second heating cover board, are provided on first heating cavity and the second heating cavity side wall for dye liquor
By curved channel.
By the heating element of dye liquor, it is not good enough to solve existing automatic staining instrument hot air type heating effect, it is possible to create gas
The pollution problem of colloidal sol.
Further, the overtemperature guarantor for being used to implement overtemperature prote effect is provided on the outer wall of the described second heating cover board
Shield switch；The temperature probe for detecting dye liquor temperature is provided on second heating cavity；The heating element passes through
Liquid line connects respectively with color solution bottle and water dropper fixed bin.
Further, it is provided with to show the aobvious of automatic staining working state of device, dye liquor surplus on the housing
Display screen.
Further, pot in the dyeing for collecting dye liquor waste material is provided in the housing.
The utility model simulated to the greatest extent using the design of drop dye component, eccentric unit it is artificially colored, and using only
Special liquid conveying and control model can relatively accurately be dropped in dye liquor on slide sample, and contaminate by program control
Liquid energy covers entire sample, and reagent consumption is reduced while preferable coloring is reached；Since whole process does not have hot wind
Program, it is consistent with the environment high of manual staining process, so the also generation in pollution-free source.
" description of the drawings "
The utility model will illustrate by example and with reference to the appended drawing, wherein：
Fig. 1 is the overall structure diagram of automatic staining device；
Fig. 2 is the explosive view of Fig. 1；
Fig. 3 is the structure diagram for dripping dyeing equipment；
Fig. 4 is the structure diagram of drop dye component；
Fig. 5 is the exploded perspective view of eccentric unit；
Fig. 6 is the exploded perspective view of heating element.
" specific embodiment "
Below in conjunction with the accompanying drawings, the utility model is described in detail.
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, the present invention is further described in detail.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.
As shown in Figure 1 and Figure 2, a kind of automatic staining device, including housing 100, the drop dyeing equipment being installed in housing 100
200 and control drop dyeing equipment operation microcontroller (not shown).It is equipped with and is used in the outer wall both sides of the housing 100
Automatically the liquid sensing element 101 of dye liquor surplus in color solution bottle 102 is detected, is loaded respectively in left and right sides color solution bottle 101 useful
In Grain stain and the reagent of acid-fast stain, it is also equipped in the both sides of housing 100 and the corresponding pump 106 of color solution bottle 102, pump
106 are connected (not shown) by liquid line with each color solution bottle 102.Meanwhile it is provided in the upper end of housing 100 conveniently
User picks and places the upper cover 104 of glass slide, by ON/OFF upper cover 104, can prevent toxic gas in dyeing course from distributing.In housing
Pot 105 in the dyeing for collecting dye liquor waste material is additionally provided in 100, pot 105 is to collect dyeing course to generate waste liquid in dyeing
Container, bottom connect a hose, waste liquid is guided；Pot installation is connected with shell in dyeing, and intermediate and eccentric unit does not have
There is contact；Special processing, surface plating Teflon so that interior pot will not be corroded by dye liquor, Er Qierong have been done in pot surface in dyeing
Easy drainage.
By being separately mounted to the liquid sensing element 101 of instrument both sides, the reagent needed for Grain stain and acid-fast stain
It is placed on liquid sensing element 101 by color solution bottle 102, mechanical switch there are one dresses under the bottle position of each color solution bottle 102, when
When bottle equipped with dye liquor is placed on liquid sensing element 101, mechanical switch is depressed by the weight of dye liquor and bottle itself, is opened
Shutdown is opened, and microcontroller receives signal, through processing, is transmitted to 103 on display screen；" reagent is insufficient " signal of display screen 103
It releases, instrument can normal dyeing；When the dye liquor of reagent bottle is almost finished (also surplus 10ml of bottle bottom or so), machinery is opened
The strength of spring jacks up bottle in pass, and display screen 103 can show certain dye liquor " reagent is insufficient ", and user is prompted to need
It replaces or increases dye liquor.
As shown in figure 3, the drop dyeing equipment 200 includes, the drop that drop dye processing is carried out to sample contaminates component 300, drop dye portion
Part 300 is two, and respectively to being applied to needed for Grain stain and acid-fast stain, drop dye component 300 passes through liquid line and dye liquor
Bottle 102 connects；The eccentric unit 400 of centrifugal treating is carried out to sample, is provided in the upper end of eccentric unit 400 and is carried for installing
The turntable of slide；The drippage that the drop dye component 300 precisely, is measured dye liquor by monolithic function is on glass slide.Wherein, it drips
Component 300, eccentric unit 400, heating element 500 is contaminated to be mounted on the larger substrate in 100 bottom of housing.
As shown in figure 4, the drop dye component 300 includes, supporting rack 301；It is installed on the guide post 302 of 301 upper end of supporting rack；
It is installed on the lead screw motor 303 of 301 side of supporting rack；Be installed on the sliding block 304 of 301 opposite side of supporting rack, the sliding block 304 with
The output terminal of lead screw motor 303 is connected and is sheathed on guide post 302, and the sliding block 304 can be along guide post by lead screw motor 303
302 length direction movement；The water dropper fixed bin 305 of dropping liquid processing is carried out to glass slide, the water dropper fixed bin 305 is fixed on
It, can be separate by water dropper fixed bin described in sliding block 304 305 or close to turntable on sliding block 304.Wherein, have in water dropper fixed bin 305
Multiple liquid droping heads.
Drop dye component 300 have with the corresponding liquid droping head of color solution bottle, connected by pipeline between them, filled among every pipeline
There are one pumps 106, when pumping 106 rotation, the dye liquor in color solution bottle 102 are transported to liquid droping head, dye liquor drips to load down automatically
On slide, so as to complete a liquid feeding process.Liquid droping head has two rows, and often there are three row, liquid droping head will be directed at each slide institute
The dye liquor that need to add by the rotation of program control lead screw motor 303, pushes and pulls stretching back and forth for liquid droping head, thus dye liquor accurately
It drips on the sample of glass slide.Its control program is：Microcontroller send instructions-motor driving-linear motor screw rotation-
Dye liquor is accurately added on sample.
As shown in figure 5, the eccentric unit 400 includes, electric machine support 401 and support for rotary disc 402；It is installed on support for rotary disc
Transmission mechanism 403 on 402；The stepper motor 404 being installed on electric machine support 401, the output terminal of the stepper motor 404 lead to
Synchronous belt is crossed to be sequentially connected with transmission mechanism 403；Shaft 405, the shaft 405 are rotatablely connected with transmission mechanism 403；Turntable
406, the turntable 406 is installed on the upper end of shaft 405, is uniformly provided with to place glass slide on the periphery of turntable 406
Slide slot 407
Eccentric unit 400 is located at the centre position of instrument, is connected with bottom plate, and the power offer of centrifugation is stepper motor
404, power is finally passed to by belt wheel transmission by turntable 406, the diameter of turntable 406 carries glass probably in 400mm or so
Piece is placed on 360 ° of uniformly distributed slide slots, and the maximum speed of turntable 406 is enough to probably at 300 revs/min the liquid on glass slide
It gets rid of, so as to reach expected coloring.Its control program is：Microcontroller send instructions-motor driving-motor rotation.
This staining instruments is dyed by the way of dropping liquid centrifugation, and slide lies against round individual layer dyeing turntable successively,
Reagent by pumping device from color solution bottle is extracted out and passes through liquid droping port and drops in a certain amount of dye liquor on slide by dyeing liquor.It is logical
The injection rate for crossing software program setting control various reagents and the residence time on dyeing turntable, then slide is gone by centrifugal drying
On liquid, finally to reach expected dyeing purpose.
As shown in Fig. 3, Fig. 6, the automatic staining device further includes the heating element for being heated to dye liquor
500；The heating element 500 includes stacking gradually the first heating cover board 501 of arrangement, the first heating plate 502, the first heating chamber
Body 503, the second heating cavity 504, the second heating plate 505 and the second heating cover board 506, in 503 He of the first heating cavity
Be provided on second heating cavity, 504 side wall for dye liquor by curved channel 509.In the described second heating cover board 506
The overtemperature prote switch 507 for being used to implement overtemperature prote effect is provided on outer wall；It is set on second heating cavity 504
It is useful for the temperature probe 508 of detection dye liquor temperature；The heating element by liquid line respectively with color solution bottle 102 and water dropper
Fixed bin 305 connects.
Heating element 400 is connected with one of which dye liquor (A agent) pipeline.The switch of heating element 400 is by microcontroller control
System, when heating element 400 is begun to warm up, temp probe 508 also begins to detection heating temperature, when being heated beyond set temperature (50
DEG C) when, temperature probe 508 detects overtemperature, and signal passes to microcontroller, and microcontroller, which sends out instruction, allows heating element 400 to stop
Only heat, when temperature is less than set temperature (50 DEG C), heating element 400 is begun to warm up again, repeatedly such break-make heating, so as to
Make temperature relatively accurately constant in the range of set temperature.
Operation principle：
Microcontroller performs the sequencing of a component according to the program of setting (there are two sets of dyeing procedures for monolithic).
Such as plus Grain stain reagent A agent：First, eccentric unit 400 causes turntable 406 to rotate, Photoelectric Detection to base
Then (zero) on schedule turns to the water dropper fixed bin 305 in the glass slide alignment drop dye component 400 of required liquid feeding；Program control
Liquid droping head processed stretches out, and stops when reaching liquid droping head alignment specimen location；Heating element 500 is begun to warm up simultaneously, when being heated to set
When constant temperature is spent, pump 106 starts drawing liquid, and dye liquor just can be dripped to directly on sample, and liquid volume added is controlled by program, thus completed
The liquid feeding process of A agent.
After the completion of liquid feeding, microcontroller control 400 high-speed rotation of eccentric unit completes dye liquor process.
Above example is only fully to disclose and unrestricted the utility model, all creation purports based on the utility model,
The replacement of equivalence techniques feature without creative work should be considered as the range of the application exposure.
Claims (8)
1. a kind of automatic staining device, including housing, the drop dyeing equipment being installed in housing and control drop dyeing equipment operation
Microcontroller, it is characterised in that：The drop dyeing equipment includes, and the drop that drop dye processing is carried out to sample contaminates component；Sample is carried out from
The eccentric unit of heart processing, the turntable for placing glass slide is provided in the upper end of eccentric unit；The drop dye component passes through
The drippage that monolithic function precisely, measures dye liquor is on the sample of glass slide；
The drop dye component includes, supporting rack；It is installed on the guide post of supporting rack upper end；It is installed on the screw electricity of supporting rack side
Machine；It is installed on the sliding block of supporting rack opposite side, the output terminal connection of the sliding block and lead screw motor and is sheathed on guide post, it is described
Sliding block can be moved by lead screw motor along the length direction of guide post；The water dropper fixed bin of dropping liquid processing is carried out to glass slide, it is described
Water dropper fixed bin is fixed on sliding block, can be separate by water dropper fixed bin described in sliding block or close to turntable.
2. automatic staining device as described in claim 1, it is characterised in that：The eccentric unit includes, electric machine support and turn
Disc carrier；The transmission mechanism being installed on support for rotary disc；The stepper motor being installed on electric machine support, the stepper motor it is defeated
Outlet is sequentially connected by synchronous belt and transmission mechanism；Shaft, the shaft are rotatablely connected with transmission mechanism；Turntable, described turn
Disk is installed on the upper end of shaft, and the slide slot for placing glass slide is uniformly provided on the periphery of turntable.
3. automatic staining device as claimed in claim 2, it is characterised in that：The automatic staining device further includes to place
The liquid sensing element of color solution bottle, the liquid sensing element are installed on housing exterior walls both sides, and the liquid sensing element can be certainly
Dye liquor surplus in dynamic detection color solution bottle.
4. automatic staining device as claimed in claim 3, it is characterised in that：The drop dye component is two；By being installed on
Pump and liquid line on housing, the water dropper fixed bin are connected with color solution bottle.
5. the automatic staining device as described in claim 1-4 any one, it is characterised in that：The automatic staining device also wraps
Include the heating element for being heated to dye liquor；The heating element includes the first heating cover for stacking gradually arrangement
Plate, the first heating plate, the first heating cavity, the second heating cavity, the second heating plate and the second heating cover board, add described first
Be provided on hot cavity and the second heating cavity side wall for dye liquor by curved channel.
6. automatic staining device as claimed in claim 5, it is characterised in that：It is set on the outer wall of the described second heating cover board
It is useful for realizing the overtemperature prote switch of overtemperature prote effect；It is provided with to detect dye liquor temperature on second heating cavity
The temperature probe of degree；The heating element is connected by liquid line with color solution bottle and water dropper fixed bin respectively.
7. automatic staining device as claimed in claim 6, it is characterised in that：It is provided on the housing automatic for showing
The display screen of dyeing apparatus working condition, dye liquor surplus.
8. automatic staining device as claimed in claim 6, it is characterised in that：It is provided with to collect dye liquor in the housing
Pot in the dyeing of waste material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721632307.9U CN207585989U (en) | 2017-11-30 | 2017-11-30 | A kind of automatic staining device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721632307.9U CN207585989U (en) | 2017-11-30 | 2017-11-30 | A kind of automatic staining device |
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| Publication Number | Publication Date |
|---|---|
| CN207585989U true CN207585989U (en) | 2018-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721632307.9U Active CN207585989U (en) | 2017-11-30 | 2017-11-30 | A kind of automatic staining device |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107957364A (en) * | 2017-11-30 | 2018-04-24 | 珠海贝索生物技术有限公司 | A kind of automatic staining device |
| CN110132695A (en) * | 2019-05-27 | 2019-08-16 | 福州迈新生物技术开发有限公司 | A kind of pathological staining system reduction human error is caused to dye abnormal method |
| CN114509325A (en) * | 2020-11-17 | 2022-05-17 | 苏州百源基因技术有限公司 | Full-automatic dyeing instrument |
| WO2023088014A1 (en) * | 2021-11-22 | 2023-05-25 | 中国科学院微生物研究所 | Transfer-type high-throughput ultrathin section staining instrument |
| CN116499844A (en) * | 2023-06-21 | 2023-07-28 | 遂宁市中心医院 | Automatic dyeing analysis device for pathological diagnosis |
-
2017
- 2017-11-30 CN CN201721632307.9U patent/CN207585989U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107957364A (en) * | 2017-11-30 | 2018-04-24 | 珠海贝索生物技术有限公司 | A kind of automatic staining device |
| CN110132695A (en) * | 2019-05-27 | 2019-08-16 | 福州迈新生物技术开发有限公司 | A kind of pathological staining system reduction human error is caused to dye abnormal method |
| CN110132695B (en) * | 2019-05-27 | 2022-01-11 | 福州迈新生物技术开发有限公司 | Method for reducing abnormal dyeing caused by human error by pathological dyeing system |
| CN114509325A (en) * | 2020-11-17 | 2022-05-17 | 苏州百源基因技术有限公司 | Full-automatic dyeing instrument |
| CN114509325B (en) * | 2020-11-17 | 2025-09-16 | 苏州百源基因技术有限公司 | Full-automatic dyeing instrument |
| WO2023088014A1 (en) * | 2021-11-22 | 2023-05-25 | 中国科学院微生物研究所 | Transfer-type high-throughput ultrathin section staining instrument |
| CN116499844A (en) * | 2023-06-21 | 2023-07-28 | 遂宁市中心医院 | Automatic dyeing analysis device for pathological diagnosis |
| CN116499844B (en) * | 2023-06-21 | 2023-09-15 | 遂宁市中心医院 | Automatic dyeing analysis device for pathological diagnosis |
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