CN207114473U - A kind of capillary electrophoresis - Google Patents

Abstract

The utility model belongs to the technical field of separation and detection devices, and relates to a capillary electrophoresis device, which includes a constant temperature box, a first buffer bottle, a sample bottle, and a second buffer bottle are arranged in the constant temperature box, and a second buffer bottle is arranged in the first buffer bottle. A transfer tube, the sample bottle is provided with a second transfer tube, the second buffer bottle is provided with a third transfer tube, the first transfer tube is provided with a first high-pressure pump, and the second transfer tube is provided with a second high-pressure pump , the output ends of the first high-pressure pump and the second high-pressure pump are connected with the input ends of the three-way valve, the output ends of the three-way valve are connected with the quantitative injection valve, the quantitative injection valve is connected with one end of the separation capillary, and the other end of the separation capillary One end is connected with the third transmission tube, the two ends of the separation capillary are respectively provided with electrodes, a high-voltage power supply is provided between the electrodes, a window is opened on the separation capillary, the optical path of the detector is opposite to the window, and the detector is connected with a data recording and processing instrument. The utility model has the advantages of simple structure and convenient use; the reproducibility of separation and detection is improved.

Description

A capillary electrophoresis device

technical field

The utility model belongs to the technical field of separation and detection devices, and relates to a capillary electrophoresis device.

Background technique

The so-called electrophoresis refers to the movement of charged particles in an electric field. Different substances move at different speeds in the electric field due to their different charges and molecular weights. According to these characteristics, the electrophoresis method can be used for qualitative or quantitative analysis of different substances. , or a certain mixture for component analysis or single component extraction preparation, which is of great significance in clinical testing or experimental research. The electrophoresis instrument is designed and manufactured based on the above principles. Electrophoresis is generally divided into two categories: free interface electrophoresis and zone electrophoresis. Free interface electrophoresis does not require supports, such as isoelectric focusing electrophoresis, isotachine electrophoresis, density gradient electrophoresis, and microelectrophoresis. This type of electrophoresis is rarely used at present. ; Zonal electrophoresis uses supporting media, which can be divided into filter paper electrophoresis, cellulose acetate membrane electrophoresis, thin layer electrophoresis and gel electrophoresis according to different supporting media. Plate electrophoresis, vertical plate electrophoresis, vertical disc electrophoresis, capillary electrophoresis, bridge electrophoresis and continuous flow electrophoresis, etc.

Capillary electrophoresis is one of the fastest-growing analytical methods in recent years. Capillary electrophoresis, also known as high-efficiency capillary electrophoresis, is a new type of liquid phase separation technology with capillary as the separation channel and high-voltage direct current electric field as the driving force. Capillary electrophoresis is widely used in the identification of grain proteins. The traditional SDS-PAGE and 2-DE methods are difficult to achieve high-throughput scanning due to the time-consuming steps of gel preparation, electrophoresis and color development, and strict requirements on the amount of test samples. Capillary electrophoresis (Capillary Electrophoresis, CE) can effectively make up for the above problems, with its micro, fast and highly automated characteristics, it shows great advantages in the batch identification of proteins.

Capillary electrophoresis technology can be used to quickly and easily identify wheat high-molecular-weight glutenin subunits (HMW-GS), which is fast and sensitive, and can efficiently and quickly determine the high-molecular-weight glutenin subunit combinations of a large number of different varieties. The currently used devices generally have the problems of complex structure and poor reproducibility of separation and detection. Therefore, it is very important to develop capillary electrophoresis devices with simple structure and high reproducibility.

Utility model content

The purpose of the utility model is to provide a capillary electrophoresis device, which solves the problems of complex structure and poor reproducibility of separation and detection in the prior art.

The technical solution adopted by the utility model is that a capillary electrophoresis device includes a thermostat, and the thermostat is provided with a first buffer bottle, a sample bottle, a second buffer bottle, a first transfer tube, a second transfer tube, The third transfer pipe, the first high-pressure pump, the second high-pressure pump, the three-way valve, the separation capillary, the quantitative sampling valve, the electrode and the detector;

The first buffer bottle is provided with a first transfer tube, the sample bottle is provided with a second transfer tube, the second buffer bottle is provided with a third transfer tube, the first transfer tube is provided with a first high-pressure pump, and the second transfer tube is provided with a second transfer tube. The transmission pipe is provided with a second high-pressure pump, the output ends of the first high-pressure pump and the second high-pressure pump are connected with the input ends of the three-way valve, the output end of the three-way valve is connected with the quantitative injection valve, and the quantitative injection valve It is connected to one end of the separation capillary, and the other end of the separation capillary is connected to the third transmission tube. Electrodes are respectively arranged at both ends of the separation capillary, and a high-voltage power supply is arranged between the two electrodes. The high-voltage power supply is connected to the two electrodes, and the separation capillary A window is opened on the top, the optical path of the detector is opposite to the window, and the detector is connected with a data recording and processing instrument.

The utility model is also characterized in that,

Wherein the first buffer bottle and the second buffer bottle are filled with buffer, and the buffer is H ₃ PO ₄ -Glycine buffer. Wherein the H ₃ PO ₄ -Glycine buffer is 20% ACN+0.4% Glycine+0.05% HPMC. Wherein the buffer is adjusted to pH 2.5 with phosphoric acid solution (85%).

The separation capillary is a quartz non-coated capillary with a detection length of 37 cm and an inner diameter of 50 μm.

Wherein the detector is a photodiode array detector.

Wherein the temperature in the thermostat is 30°C.

The beneficial effects of the utility model are: simple structure, easy to use; the sampling method of the quantitative sampling valve makes the sampling volume accurate and highly repeatable, and the system with buffer solution significantly improves the reproducibility of separation detection; The liquid and the sample are divided into two inputs, which makes it easy and convenient to repeatedly test the same sample between two tests, improves the detection efficiency, and improves the reproducibility of separation and detection at the same time.

Description of drawings

Fig. 1 is a schematic structural view of a capillary electrophoresis device of the present invention.

Explanation of reference signs:

1. Thermostat, 2. First buffer bottle, 3. Sample bottle, 4. Second buffer bottle, 5. First transfer tube, 6. Second transfer tube, 7. Third transfer tube, 8. Second transfer tube 1. High-pressure pump, 9. Second high-pressure pump, 10. Three-way valve, 11. Separation capillary, 12. Quantitative sampling valve, 13. Electrode, 14. Detector, 15. High-voltage power supply, 16. Data recorder.

Detailed ways

A specific embodiment of the present invention will be described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiment.

A capillary electrophoresis device, as shown in Figure 1, comprises an incubator 1, and the incubator 1 is provided with a first buffer bottle 2, a sample bottle 3, a second buffer bottle 4, a first transfer tube 5, a second transfer tube Pipe 6, third transfer pipe 7, first high pressure pump 8, second high pressure pump 9, three-way valve 10, separation capillary 11, quantitative sampling valve 12, electrode 13 and detector 14;

The first buffer bottle 2 is provided with a first transfer tube 5, the sample bottle 3 is provided with a second transfer tube 6, the second buffer bottle 4 is provided with a third transfer tube 7, and the first transfer tube 5 is provided with The first high-pressure pump 8, the second transmission pipe 6 is provided with a second high-pressure pump 9, the output end of the first high-pressure pump 8 and the second high-pressure pump 9 are connected with the input end of the three-way valve 10, the three-way valve 10 The output end is connected to the quantitative sampling valve 12, the quantitative sampling valve 12 is connected to one end of the separation capillary 11, the other end of the separation capillary 11 is connected to the third transfer pipe 7, and the two ends of the separation capillary 11 are respectively provided with electrodes 13. A high-voltage power supply 15 is arranged between the two electrodes 13, the high-voltage power supply 15 is connected to the two electrodes 13, a window is opened on the separation capillary 11, the optical path of the detector 14 is opposite to the window, and the detector 14 is connected to a data recorder 16 .

The injection method of the quantitative injection valve makes the injection volume accurate and repeatable. By dividing the buffer solution and the sample into two channels, the operation of repeated testing of the same sample between two tests becomes simple and convenient, the detection efficiency is improved, and the reproducibility of separation detection is improved at the same time.

Wherein the first buffer bottle 2 and the second buffer bottle 4 are both filled with buffer, and the buffer is H ₃ PO ₄ -Glycine buffer. Wherein the H ₃ PO ₄ -Glycine buffer is 20% ACN+0.4% Glycine+0.05% HPMC. Wherein the buffer is adjusted to pH 2.5 with phosphoric acid solution (85%). The injection method of the quantitative injection valve and the system of the buffer solution significantly improved the reproducibility of the separation and detection.

Wherein the separation capillary 11 is a quartz non-coated capillary with a detection length of 37 cm and an inner diameter of 50 μm.

Wherein the detector 14 is a photodiode array detector.

Wherein the temperature in the incubator 1 is 30°C.

The separation voltage of the separation detection is 12.5kv.

Capillary electrophoresis is an electrophoretic separation analysis method that uses a capillary as a separation channel and a high-voltage direct current electric field as a driving force to achieve separation based on the differences in mobility and distribution behavior between components in a sample. Principle: The solution to be analyzed is introduced into the sampling end of the capillary, and after applying DC voltage, each component flows to the outlet end of the capillary according to the vector sum of their electrophoretic flow and electroosmotic flow, in the order of cations, neutral ions, anions and their charges The neutral components cannot be separated from each other by the detector.

When in use, open the incubator 1 and set its internal temperature to 30°C, add buffer solution in the first buffer solution bottle 2 and the second buffer solution bottle 4, add the sample to be tested in the sample bottle 3, and turn on the first high pressure pump 8, suck the buffer solution from the first buffer solution bottle 2 into the first transfer pipe 5, open the three-way valve 10 to the corresponding position, the buffer solution enters the entire separation capillary 11, and then turn on the second high-pressure pump 9 to transfer the sample Inhale the second transfer pipe 6 from the sample bottle 3, open the three-way valve 10 to the corresponding position, control the sample injection volume with the quantitative sampling valve 12, the sample enters the inlet end of the separation capillary 11, turn on the high voltage power supply 15, and the separation voltage is 12.5kv, each component flows to the outlet end of the separation capillary 11 according to the vector sum of its electrophoretic flow and electroosmotic flow, and is detected and separated by the detector 14 successively according to the ion characteristics.

In summary, the capillary electrophoresis device provided by the embodiment of the utility model has a simple structure and is easy to use; the sampling method of the quantitative sampling valve 12 makes the sampling volume accurate and repeatable, and the system with the buffer solution is significantly improved. The reproducibility of separation detection; by dividing the buffer and the sample into two channels, the operation of repeating the same sample between two tests becomes simple and convenient, which improves the detection efficiency and improves the reproducibility of separation detection at the same time.

The above disclosures are only specific embodiments of the present utility model, but the embodiments of the present utility model are not limited thereto, and any changes conceivable by those skilled in the art shall fall within the protection scope of the present utility model.

Claims (4)

1. a kind of capillary electrophoresis, it is characterised in that including insulating box (1), it is slow that first is provided with the insulating box (1) Fliud flushing bottle (2), sample bottle (3), the second buffer solution bottle (4), the first transfer tube (5), the second transfer tube (6), the 3rd transfer tube (7), the first high-pressure pump (8), the second high-pressure pump (9), triple valve (10), separation capillary (11), quantitative sample injection valve (12), electrode And detector (14) (13)；

The first transfer tube (5) is provided with the first buffer solution bottle (2), the second transfer tube (6) is provided with the sample bottle (3), The 3rd transfer tube (7) is provided with the second buffer solution bottle (4), first transfer tube (5) is provided with the first high-pressure pump (8), Second transfer tube (6) is provided with the output end of the second high-pressure pump (9), first high-pressure pump (8) and the second high-pressure pump (9) It is connected with the input of triple valve (10), the output end of the triple valve (10) is connected with quantitative sample injection valve (12), described Quantitative sample injection valve (12) is connected with separating one end of capillary (11), and the other end of the separation capillary (11) and the 3rd passes Defeated pipe (7) is connected, and the both ends of the separation capillary (11) are respectively arranged with electrode (13), between two electrodes (13) High voltage power supply (15) is provided with, the high voltage power supply (15) is connected with two electrodes (13), is opened up on the separation capillary (11) There is window, the light path of the detector (14) is relative with window, and the detector (14) is connected with data recording and processing instrument (16).

2. a kind of capillary electrophoresis as claimed in claim 1, it is characterised in that the first buffer solution bottle (2) and the Buffer solution is respectively arranged with two buffer solution bottles (4).

3. a kind of capillary electrophoresis as claimed in claim 1, it is characterised in that the separation capillary (11) is detection Length is 37cm, and internal diameter is 50 μm of quartzy non-coatings capillary pipe.

4. a kind of capillary electrophoresis as claimed in claim 1, it is characterised in that the detector (14) is the pole of photoelectricity two Pipe array detector.