CN206916128U - A kind of improved zooblast reaction unit - Google Patents

Abstract

The utility model discloses an improved animal cell reaction device, which comprises a reaction vessel and a stirrer. The upper part of the stirrer is connected with a connecting part, and a second air cavity is connected inside the connecting part, and two second air chambers are arranged on the inner wall of the connecting part. Two concave rings, the second concave ring is in sliding contact with a convex ring, the convex ring is connected on the outer wall of the first air intake pipe, and the first concave ring is also arranged on the outer wall of the first air intake pipe, the first concave ring is connected with the first air intake pipe by a steel ball The inner wall is in sliding contact, and several stirring rods are connected to the outer wall of the connecting part. The stirring rod is connected with a second air intake pipe inside, and a slider is in sliding contact with the second air intake pipe. There is a first fixed chamber, the first air chamber is connected to the lower part of the first fixed chamber, and several air outlets are evenly arranged on the stirring rod. The effect of cell damage.

Description

An improved animal cell reaction device

technical field

The utility model relates to the field of biological experiment equipment, in particular to an improved animal cell reaction device.

Background technique

In recent years, the development of animal cell culture technology has greatly promoted the development of modern biomedicine industry. This technology is widely used in the preparation of monoclonal antibodies, the research and development of recombinant protein drugs, artificial organ transplantation and other industries, and has become one of the key technologies in the field of biopharmaceuticals. With the rapid growth of global demand for biological products, large-scale animal cell culture technology is becoming more and more popular, and as an irreplaceable main equipment in the large-scale cultivation of animal cells - bioreactor, in the production of biological products It also plays an increasingly important role in production. It can effectively increase the culture density of cell unit volume, thus laying a solid foundation for the large-scale production of animal cells. There are various types of animal cell culture reactors, and the most commonly used ones are mechanical stirring type, torrent type, air lift type, disposable bag type, cage type aeration stirring and fixed bed type, etc. Among them, the research on stirred animal cell reactor is more important. Mature, its operation is similar to that of microbial fermentation tank, and the process operation is simple and easy, which has become the mainstream trend of biological product production. Chinese utility model patent CN 204569941 U discloses an animal cell bioreactor. The air intake system and the stirring system are separated, so that incomplete contact between the cells and the gas may occur during the reaction, resulting in incomplete reaction. May cause damage to cells with adverse effects on cellular responses.

Utility model content

The technical problem to be solved by the utility model is to provide an improved animal cell reaction device, which can solve the deficiencies of the prior art, and has a more sufficient reaction and a good effect of reducing cell damage.

In order to solve the problems of the technologies described above, the technical solutions adopted by the utility model are as follows.

An improved animal cell reaction device, including a reaction vessel and a stirrer, the upper part of the stirrer is connected with a connecting part, the connecting part is connected with a second air cavity inside, and two second concave rings are arranged on the inner wall of the connecting part. The two concave rings are in sliding contact with a protruding ring, the protruding ring is connected on the outer wall of the first air intake pipe, the outer wall of the first air intake pipe is also provided with a first concave ring, the first concave ring is located between the two protruding rings, the first concave ring Through the sliding contact of the steel ball with the inner wall of the first air intake pipe, the first air intake pipe is connected with the second air cavity, and several stirring rods are connected on the outer wall of the connection part, and the second air intake pipe is connected inside the stirring rod, and the second air intake pipe and the second The two air chambers are connected through each other, and there is a sliding block in the second air intake pipe, which is connected to the bottom of the second air intake pipe through a spring, and the first fixed chamber is connected to the outside of the end of the second air intake pipe connected to the second air chamber. A first air chamber is connected to the lower part of the fixed chamber, and the first air chamber is connected through the air inlet to the second air inlet pipe. Several air outlets are uniformly arranged on the stirring rod, and the air outlets are connected to the first air chamber.

Preferably, the number of the stirring rod is at least one, and the angle between the stirring rod and the connecting part is 30°.

Preferably, there are at least one gas blowing port on the stirring rod, and the angle between the gas outlet and the horizontal direction is greater than 45°.

Preferably, a filter is connected between the second air intake pipe and the first air cavity.

The beneficial effects brought about by adopting the above technical solution are: the utility model is provided with a connecting part, the connecting part is connected with a second air cavity inside, two second concave rings are arranged on the inner wall of the connecting part, and the second concave rings are in sliding contact with each other. There is a convex ring, the convex ring is connected on the outer wall of the first air intake pipe, and the first concave ring is also arranged on the outer wall of the first air intake pipe, the first concave ring is located between the two convex rings, the first concave ring is connected with the first The inner wall of the air intake pipe is in sliding contact, and the first air intake pipe is connected through the second air cavity, which ensures the tightness of the connection between the first air intake pipe and the connecting part, and also realizes that when the connecting part rotates under the agitator, The first air intake pipe can be kept fixed and can be ventilated in real time. At the same time, a number of stirring rods are connected to the outer wall of the connecting part, and a second air intake pipe is connected inside the stirring rod, and the second air intake pipe is connected through the second air cavity. There is a sliding block in the sliding contact of the second air intake pipe, which is connected to the bottom of the second air intake pipe through a spring. It is difficult to slide down. When the slider does not move, no gas can enter the air inlet. The end of the second air inlet pipe connected to the second air chamber is connected to the first fixed chamber. The lower part of the first fixed chamber Connected with the first air chamber, the first air chamber is connected through the air inlet with the second air inlet pipe, and several air outlets are uniformly arranged on the stirring rod, and the air outlets are connected through the first air chamber. During the experiment, the stirrer Driven by the motor, it continues to rotate. After the stirrer rotates, it drives the connecting part to rotate, and the connecting part drives the stirring rod to continue rotating for stirring. When the two connecting parts are rotating at the same time, the first air inlet pipe remains still and continues to supply the second air cavity. Gas, after the gas in the second air chamber enters the second air intake pipe, the slider in the second air pipe slides down and leaks out of the air inlet under the simultaneous action of the centrifugal force of the stirring rod rotation and the air intake pressure, so that the The gas port continuously supplies gas to the first air cavity, and the gas in the first righteous gas cavity continuously enters the reaction vessel through the gas outlet to react with the cells. The cells in the reaction vessel seldom come into contact with the stirring rod, so that when the stirring rod continues to stir, it can not only achieve a sufficient reaction effect, but also reduce the contact between the cells and the stirring rod, reduce the probability of cell damage, and achieve In order to achieve a good protection effect, further, the number of stirring rods is at least 30, and the angle between the stirring rod and the connecting part is 30°, so that a better stirring effect can be achieved, and the cells will not be driven to sputter upwards , further, the number of air outlets is at least 100, and the angle between the air outlet and the horizontal direction is greater than 45°, which can achieve a better reaction effect, and at the same time, the air outlet is oblique, and the cells are not easy to enter the air outlet. Further A filter screen is provided in the first air cavity, so that the impurities in the blown gas are filtered to achieve a better reaction effect.

Description of drawings

Fig. 1 is a structural diagram of a specific embodiment of the present invention.

Fig. 2 is a sectional view of the connection of the intake pipe in a specific embodiment of the present invention.

Fig. 3 is a sectional view of the connection of the agitator in a specific embodiment of the present invention.

Fig. 4 is a cross-sectional view of a stirring rod in a specific embodiment of the present invention.

Fig. 5 is a cross-sectional view of the air outlet of the stirring rod in a specific embodiment of the present invention.

Fig. 6 is a side view of a stirring rod in a specific embodiment of the present invention.

Fig. 7 is a sectional view of the connection of the soft brush in a specific embodiment of the present invention.

Fig. 8 is a front view of a soft brush head in a specific embodiment of the present invention.

In the figure: 1. Reaction vessel; 2. Stirrer; 3. Connecting part; 4. Stirring rod; 5. First air inlet pipe; 6. Convex ring; 7. First concave ring; 8. Steel ball; 9. Second Concave ring; 10, second air intake pipe; 11, slider; 12, spring; 13, first fixed cavity; 14, air inlet; 15, filter screen; 16, air outlet; 17, first fixed rod; 18 , the second fixed cavity; 19, the drawstring; 20, the second fixed rod; 21, the guide wheel; 22, the soft brush; 23, the rotating shaft; 24, the soft brush head; 25; the first air chamber; 26; the second air cavity.

detailed description

The standard parts used in the utility model can be purchased from the market, and the special-shaped parts can be customized according to the instructions and the records of the accompanying drawings. The specific connection methods of each part adopt mature bolts, rivets, Conventional means such as welding and pasting are not described in detail here.

Referring to Figures 1-8, a specific embodiment of the present invention includes a reaction vessel 1 and a stirrer 2, the upper part of the stirrer 2 is connected with a connecting part 3, the connecting part 3 is connected with a second air cavity 26 inside, and the inner wall of the connecting part 3 Two second concave rings 9 are arranged on the top, and the second concave ring 9 is in sliding contact with the convex ring 6. The convex ring 6 is connected to the outer wall of the first air intake pipe 5, and the outer wall of the first air intake pipe 5 is also provided with a first concave ring. 7. The first concave ring 7 is located between the two convex rings 6. The first concave ring 7 is in sliding contact with the inner wall of the first air intake pipe 5 through the steel ball 8. The first air intake pipe 5 is connected through the second air cavity 26. The connecting part 3 There are several stirring rods 4 connected to the outer wall, the stirring rod 4 is connected with the second air inlet pipe 10 inside, the second air inlet pipe 10 is connected with the second air cavity 26 through, and the sliding block 11 is in sliding contact with the second air inlet pipe 10, The slider 11 is connected to the bottom of the second air intake pipe 10 through a spring 12, and the outside of one end of the second air intake pipe 10 connected to the second air chamber 26 is connected to the first fixed chamber 13, and the lower part of the first fixed chamber 13 is connected to the first air chamber 25, the first air chamber 25 is connected through the air inlet 14 with the second air inlet pipe 10, and several air outlets 16 are evenly arranged on the stirring rod 4, and the air outlet 16 is connected through the first air chamber 25, and the stirring rod 4 The quantity is at least one, the angle between the stirring rod 4 and the connecting portion 3 is 30°, the blowing port on the stirring rod 4 is at least one, the angle between the air outlet 16 and the horizontal direction is greater than 45°, the second air inlet pipe 10 and the first A filter screen 15 is connected between the air chambers 25, and the utility model is provided with a connecting part 3, and a second air chamber 26 is connected inside the connecting part 3, and two second concave rings 9 are arranged on the inner wall of the connecting part 3. The two concave rings 9 are in sliding contact with the convex ring 6, the convex ring 6 is connected on the outer wall of the first air intake pipe 5, and the first concave ring 7 is also arranged on the outer wall of the first air intake pipe 5, and the first concave ring 7 is located between the two convex rings. 6, the first concave ring 7 is in sliding contact with the inner wall of the first air intake pipe 5 through the steel ball 8, and the first air intake pipe 5 is connected with the second air cavity 26, thus ensuring the connection between the first air intake pipe 5 and the connecting part 3 At the same time, when the connecting part 3 is driven by the agitator 2, the first air inlet pipe 5 can be kept fixed and can be ventilated in real time. The rod 4 and the stirring rod 4 are internally connected with a second air intake pipe 10, the second air intake pipe 10 is connected through the second air chamber 26, and a slider 11 is in sliding contact with the second air inlet pipe 10, and the slider 11 is connected to the first air inlet pipe 11 through a spring 12. The bottom of the second air intake pipe 10 is connected, and the slider 11 blocks the air inlet 14. In this way, when the ventilation pressure is insufficient and the rotation speed of the agitator 2 is insufficient, the slider 11 is difficult to slide downward, and the slider 11 does not move. In the case of the air inlet 14, no gas can enter, and the outside of the end of the second air inlet pipe 10 connected to the second air chamber 26 is connected with the first fixed chamber 13, and the lower part of the first fixed chamber 13 is connected with the first air chamber. 25. The first air chamber 25 is connected through the air inlet 14 with the second air inlet pipe 10, and the stirring rod 4 is uniformly provided with several air outlets 16, and the air outlet 16 is connected through the first air chamber 25. During the experiment, the stirrer 2 continues to rotate under the drive of the motor. After the stirrer 2 rotates, it drives the connecting part 3 to rotate, and the connecting part 3 drives the stirring rod 4 to continuously rotate for stirring. When the second connecting part 3 is rotating, the first The air intake pipe 5 remains motionless and continues to supply gas to the second air chamber 26. After the gas in the second air chamber 26 enters the second air intake pipe 10, the slider 11 in the second air pipe is rotated by the centrifugal force of the stirring rod 4 and the air intake. Under the simultaneous action of pressure, it slides down and leaks out of the air inlet 14, so that the first air chamber 25 is continuously supplied with air through the air inlet 14, and the gas in the first air chamber continuously enters the reaction chamber through the air outlet 16 React with the cells in the vessel 1, because the cells in the reaction vessel 1 are subjected to the continuous gas impulse of the gas outlet 16, the cells in the reaction vessel 1 seldom touch the stirring rod 4, so when the stirring rod 4 continues to stir , can not only achieve a sufficient reaction effect, but also reduce the contact between the cells and the stirring rod 4, reduce the probability of cell damage, and achieve a good protective effect. Further, the number of stirring rods 4 is at least 30, stirring The angle between the rod 4 and the connecting part 3 is 30°, so that while a good stirring effect can be achieved, the cells will not be driven to sputter upwards. Further, the number of gas outlets 16 is at least 100, and the gas outlets 16 and The angle in the horizontal direction is greater than 45°, which can achieve a better reaction effect. At the same time, the air outlet 16 is inclined, and the cells are not easy to enter the air outlet 16. Further, a filter screen 15 is provided in the first air cavity 25 , so that the impurities in the blown gas are filtered to achieve a better reaction effect.

In addition, since this embodiment uses the integrated structure of the stirring rod 4 and the gas outlet 16, after the experiment is completed, the remaining cells or impurities in the reaction are easy to adhere to the gas outlet 16, and in severe cases, they may accumulate more and more. In this way, in the next experiment, some gas outlets 16 may be blocked or impurities may re-enter the reaction vessel 1 under the drive of others, which will affect the reaction effect. Connected with the second fixed cavity 18, the first fixed cavity 13 is connected through the second fixed cavity 18, and several first fixed rods 17 are connected on the slider 11, and the first fixed rod 17 is connected with a pull cord 19, which is pulled The other end of rope 19 is connected with rotating shaft 23, is connected with soft brush 22 on the rotating shaft 23, is connected with several soft brush heads 24 on the soft brush 22, and soft brush head 24 contacts with air outlet 16 selectively, in the second fixed A second fixed rod 20 is connected inside the cavity 18, and a guide wheel 21 is connected to the second fixed rod 20. The guide wheel 21 has a guiding effect on the stay rope 19. During the experiment, the slider 11 is driven by centrifugal force and gas pressure. During the downward sliding process, the slider 11 drives the stay cord 19 upwards through the first fixed rod 17, and the stay cord 19 drives the soft brush 22 to move upward, so that the air outlet 16 can vent the air, and the soft brush 22 can not only help fully stir but also can After use, the air outlet 16 is blocked by the soft brush head 24 to achieve a good protection effect.

Structures such as springs and filter screens used in the utility model belong to mature parts of the prior art, and are not described in detail here.

In describing the present invention, it should be understood that the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical" , "horizontal", "top", "bottom", "inner", "outer" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the utility model, rather than Any indication or implication that a referenced device or element must have a particular orientation, be constructed, and operate in a particular orientation should not be construed as limiting the invention.

The basic principles and main features of the present utility model and the advantages of the present utility model have been shown and described above. Those skilled in the art should understand that the utility model is not limited by the above-mentioned embodiments. The above-mentioned embodiments and descriptions only illustrate the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model The new model also has various changes and improvements, and these changes and improvements all fall within the scope of the claimed utility model. The scope of protection required by the utility model is defined by the appended claims and their equivalents.

Claims (4)

1. An improved animal cell reaction device, including a reaction vessel (1) and a stirrer (2), characterized in that: the upper part of the stirrer (2) is connected with a connecting part (3), and the inside of the connecting part (3) The second air cavity (26) is connected, and two second concave rings (9) are arranged on the inner wall of the connecting part (3). The second concave ring (9) is in sliding contact with the convex ring (6), and the convex ring (6) Connected to the outer wall of the first air intake pipe (5), the outer wall of the first air intake pipe (5) is also provided with a first concave ring (7), the first concave ring (7) is located between the two convex rings (6), The first concave ring (7) is in sliding contact with the inner wall of the first air intake pipe (5) through the steel ball (8), the first air intake pipe (5) is connected through the second air cavity (26), and the outer wall of the connecting part (3) is connected There are several stirring rods (4), the stirring rods (4) are internally connected with a second air intake pipe (10), the second air intake pipe (10) is connected through the second air cavity (26), and the second air intake pipe (10) There is a slider (11) in the inner sliding contact, the slider (11) is connected to the bottom of the second air intake pipe (10) through a spring (12), and one end of the second air intake pipe (10) is connected to the second air chamber (26) on the outside The first fixed chamber (13) is connected, and the lower part of the first fixed chamber (13) is connected with the first air chamber (25), and the first air chamber (25) passes through the air inlet (14) and the second air inlet pipe (10) Through connection, several air outlets (16) are uniformly arranged on the stirring rod (4), and the air outlets (16) are connected through the first air cavity (25). 2. The improved animal cell reaction device according to claim 1, characterized in that: the number of the stirring rods (4) is at least 30, and the included angle between the stirring rods (4) and the connecting part (3) is 30° °. 3. The improved animal cell reaction device according to claim 1, characterized in that: there are at least 100 gas blowing ports (16) on the stirring rod (4), and the angle between the gas outlets (16) and the horizontal direction is Greater than 45°. 4. The improved animal cell reaction device according to claim 1, characterized in that: a filter screen (15) is connected between the second air inlet pipe (10) and the first air chamber (25).