CN206843509U - A kind of novel cell culture device - Google Patents
A kind of novel cell culture device Download PDFInfo
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- CN206843509U CN206843509U CN201720595639.8U CN201720595639U CN206843509U CN 206843509 U CN206843509 U CN 206843509U CN 201720595639 U CN201720595639 U CN 201720595639U CN 206843509 U CN206843509 U CN 206843509U
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- cell culture
- component
- culture incubator
- carbon dioxide
- liquid
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Abstract
It the utility model is related to a kind of novel cell culture device, including cell culture incubator, one with connect inside cell culture incubator enter liquid adapter and one and go out liquid adapter, and the oxygen measuring device for measuring oxygen content being arranged in cell culture incubator；Wherein it is described enter liquid adapter be connected with for control nutrient solution enter liquid flow into component, it is described go out liquid adapter be connected with for control nutrient solution flow out liquid discharge component；The liquid flows into component and liquid discharge component is electrically connected with a speed adjuster；The internal chamber wall of the cell culture incubator is fixed with the cell attachment dielectric layer of annular, and the cell attachment dielectric layer separates with the internal chamber wall；And an air inlet is additionally provided with the cell culture incubator, the air inlet is connected with carbon dioxide provisioning component and nitrogen supply (NS) component respectively by emulsion tube；The air inlet is configured with the air intake valve for controlling air inlet off and on.
Description
Technical field
It the utility model is related to technical field of experiment equipment, more particularly to a kind of novel cell culture device.
Background technology
In cell culture experiments, the frequent of nutrient solution is frequently referred to；The widely used method of domestic and foreign scholars is root
According to the time requirement of experimental design, culture medium is changed in super-clean bench, so as to simulate the condition that fluctuation sexually revises.Mesh
Before, the method relative maturity is reliable.But with the increasing that fluctuation experiment is carried out to drug concentration in culture medium or certain material
It is more, when especially relating to time point and changing intensive, gradually find existing timing to change the method for culture medium that there is many
Deficiency.
On the other hand, anoxic is the reason for causing cell and common tissue damage, thus the research of anoxic increasingly by
Concern.Design hypoxemia culture model has the function that important for medical research at this stage.
Utility model content
The purpose of this utility model is to provide a kind of novel cell culture device, to realize the changing liquid automatically of cell culture fluid
And the technique effect of sterile hypoxemia culture.
In order to realize above-mentioned technique effect, the utility model provides a kind of novel cell culture device, including cell training
Foster case, one with being connected inside cell culture incubator enter liquid adapter and one and go out liquid adapter, and the use being arranged in cell culture incubator
In the oxygen measuring device of metering oxygen content；Wherein it is described enter liquid adapter be connected with for control nutrient solution enter liquid inflow group
Part, it is described go out liquid adapter be connected with for control nutrient solution flow out liquid discharge component；The liquid flows into component and liquid
Component is discharged to be electrically connected with a speed adjuster；The internal chamber wall of the cell culture incubator is fixed with the cell attachment medium of annular
Layer, the cell attachment dielectric layer separate with the internal chamber wall；And an air inlet is additionally provided with the cell culture incubator, the air inlet
Mouth is connected with carbon dioxide provisioning component and nitrogen supply (NS) component respectively by emulsion tube；The air inlet is configured with for controlling
The air intake valve of air inlet off and on.
Further, the carbon dioxide provisioning component includes carbon dioxide charging-tank and is arranged at carbon dioxide charging-tank
The carbon dioxide flow instrument for measure control carbon dioxide supply between cell culture incubator；And the nitrogen supply (NS)
Component includes nitrogen supply (NS) tank and is arranged between nitrogen supply (NS) tank and cell culture incubator to be used for measure control nitrogen supply (NS) amount
Nitrogen flow instrument.
Further, the carbon dioxide meter for measuring carbon dioxide content is additionally provided with the cell culture incubator.
Further, it is provided with the emulsion tube positioned at the junction of emulsion tube and air inlet for the sterile of aseptic filtration
Filter membrane.
Further, the speed adjuster is provided with for inputting control signal and showing the touch display screen of time flow.
Further, the liquid feeding end of the liquid inflow component is connected by sterile medical fluid flow tube with fresh medium bottle；
And the outlet end of the liquid discharge component is connected by sterile medical fluid flow tube with waste collection bottle.
Further, the cell attachment dielectric layer is non-woven fabrics plicated layer.
Further, the novel cell culture culture apparatus also includes entering by described emulsion tube and cell culture incubator
The connected oxygen supply component of gas port；And the oxygen supply component includes oxygen supply tank and is arranged at oxygen supply tank
The oxygen flux instrument for measure control oxygen supply amount between cell culture incubator.
Further, the center of the intracavity bottom of the cell culture incubator is conformed with, and the non-woven fabrics plicated layer is existed
It is radially fixed in the inner chamber of the cell culture incubator.
This novel cell culture device beneficial effect is that Stability Analysis of Structures is reliable, can be efficient by the speed adjuster of setting
Realize the replacing of cell liquid, automaticity height.By the various gas provisioning components of setting, can control in cell culture incubator
Each gas parameter, make the growth demand for more conforming to cell.Again by adding cell attachment in the inner chamber of cell culture incubator
Dielectric layer, the surface area that cell attachment culture can be carried out in cell culture incubator is added, so as to improve cell culture incubator once
Cultivate the amount of cell.
Brief description of the drawings
The utility model is further illustrated with reference to the accompanying drawings and examples.
Fig. 1 is the structural representation of cell culture apparatus of the present utility model；
Fig. 2 is the inner chamber top view of cell culture incubator of the present utility model.
In figure：Cell culture incubator 1, enter liquid adapter 2, go out liquid adapter 3, oxygen measuring device 4, liquid inflow component 5, liquid discharge
Component 6, speed adjuster 7, cell attachment dielectric layer 8, sterilised membrane filter 9, carbon dioxide charging-tank 10, carbon dioxide flow instrument 11, nitrogen
Gas charging-tank 12, nitrogen flow instrument 13, oxygen supply tank 14, oxygen flux instrument 15, touch display screen 16, projection 17, exhaust outlet
18th, fresh medium bottle 19, waste collection bottle 20, cable tube 21.
Embodiment
The utility model is described in further detail presently in connection with accompanying drawing.These accompanying drawings are simplified schematic diagram,
Only illustrate basic structure of the present utility model in a schematic way, therefore it only shows the composition relevant with the utility model.
Embodiment 1
As depicted in figs. 1 and 2, the utility model provides a kind of novel cell culture device, including cell culture incubator 1,
One connected with the inside of cell culture incubator 1 enters liquid adapter 2 and one and goes out liquid adapter 3, and is arranged in cell culture incubator 1 and is used for
Measure the oxygen measuring device 4 of oxygen content；Wherein it is described enter liquid adapter 2 be connected with for control nutrient solution enter liquid flow into component
5, it is described go out liquid adapter 3 be connected with for control nutrient solution flow out liquid discharge component 6；The liquid flows into component 5 and liquid
Body discharge component 6 is electrically connected with a speed adjuster 7, and liquid need to can be only completed by speed adjuster 7 and flows into component 5 and liquid row
The liquid absorption for going out component 6 is set, and liquid flows into component 5 and liquid discharges component 6 under the control of same speed adjuster 7, energy
It is enough to flow into component and discharge liquid simultaneously, so as to maintain uniformity so that the liquid measure of inflow and the amount of discharge are as many.It is described
The internal chamber wall of cell culture incubator 1 is fixed with the cell attachment dielectric layer 8 of annular, the cell attachment dielectric layer 8 and the internal chamber wall
Separation；And an air inlet is additionally provided with the cell culture incubator 1, the air inlet is supplied with carbon dioxide respectively by emulsion tube
Component is connected with nitrogen supply (NS) component, it is preferred that the junction of emulsion tube and air inlet is provided with use on the emulsion tube
In the sterilised membrane filter 9 of aseptic filtration, sterilised membrane filter 9 is used for sterile to the carbon dioxide into cell culture incubator 1 and nitrogen progress
Filtering, and then ensure the gnotobasis of cell culture incubator 1；The air inlet is configured with the air inlet for controlling air inlet off and on
Valve（It is not drawn into figure）.
Preferably, in order to further ensure the gnotobasis in cell culture incubator 1, the training by entering the inflow of liquid adapter 2 is avoided
Pollution of the miscellaneous bacteria carried secretly in nutrient solution to cell culture incubator, it is described enter liquid adapter 2 and the junction of cell culture incubator 1 be provided with it is sterile
Filter membrane 9.
The cell culture incubator 1 is additionally provided with the exhaust outlet 18 for exhaust；The exhaust outlet 18, which is provided with, to be used to control exhaust outlet
The drain tap of 18 on and off.
Specifically, the carbon dioxide provisioning component includes carbon dioxide charging-tank 10 and is arranged at carbon dioxide supply
The carbon dioxide flow instrument 11 for measure control carbon dioxide supply between tank 10 and cell culture incubator 1；It is and described
Nitrogen supply (NS) component includes nitrogen supply (NS) tank 12 and is arranged between nitrogen supply (NS) tank 12 and cell culture incubator 1 to be used to measure
Control the nitrogen flow instrument 13 of nitrogen supply (NS) amount.
The carbon dioxide meter for measuring carbon dioxide content is additionally provided with the cell culture incubator 1.
The premise of the cell culture apparatus of the present embodiment is that air is filled with cell culture incubator 1, oxygen measuring device 4, dioxy
Change carbon measuring instrument, air intake valve, drain tap, carbon dioxide flow instrument 11, nitrogen flow instrument 13 with a peripheral control unit phase
Even.Controlled by the coordination of carbon dioxide provisioning component, nitrogen supply (NS) component, air intake valve and drain tap, displace cell
Air in incubator 1 so that the Control for Oxygen Content of cell culture incubator 1 is being adapted to the oxygen demand of cell culture.Specifically, pass through
Carbon dioxide provisioning component and nitrogen supply (NS) component carry out the carbon dioxide and nitrogen input quantity of control input cell culture incubator 1, and
Air in cell culture incubator 1 is exported by exhaust outlet 18, and then the oxygen in cell culture incubator 1 is measured by oxygen measuring device 4
Content, when the oxygen content in cell culture incubator 1 reaches the proportion of requirement of experiment, and then closing intake valve and air bleeding valve come
Keep gas ratio in cell culture incubator 1 constant, such as the sterile low-oxygen environment in setting cell culture incubator 1, and then to hypoxemia
Cell is cultivated.
Again specifically, the liquid feeding end that the liquid flows into component 5 passes through sterile medical fluid flow tube and fresh medium bottle 19
Connection；And the outlet end of the liquid discharge component 6 is connected by sterile medical fluid flow tube with waste collection bottle 20.
Optionally, by setting a stand type support in cell culture incubator 1（Do not marked in figure）, to fixed speed adjuster 7,
Fresh medium bottle 19 and waste collection bottle 20.Some necks are set to be respectively used to block fresh medium in the stand type support
Bottle 19 and waste collection bottle 20, make to be not easy to topple over.Cable tube 21, the control are installed with by pipe clamp in the stand type support
The communications cable that fast device 7 and liquid are flowed between component 5 and liquid discharge component 6 is encapsulated by the cable tube 21, is avoided
Cable it is in disorder, extend its service life.
Preferably, the speed adjuster 7 is provided with for inputting control signal and showing the touch display screen 16 of time flow.
Optionally, the cell attachment dielectric layer 8 is non-woven fabrics plicated layer.Using the non-woven fabrics pleat made of non-woven fabrics
Rennet can carry out the surface area of cell attachment culture further to increase in cell culture incubator 1.Using the non-woven fabrics plicated layer
Afterwards, the surface area of cell attachment culture can be carried out in cell culture incubator 1 at least increase as original five to seven times, cell culture
Cheng Zhong, cell density increase adherent in Tissue Culture Flask is original five to seven times, and cell culture incubator 1 can once be cultivated carefully
The amount of born of the same parents can increase as original five to seven times.The amount that cell culture incubator 1 can once cultivate cell can further meet to test
Requirement, cost is further reduced in medicine and medicine equipment R＆D process, and the loss of resource is reduced.Using the non-woven fabrics pleat
Another reason of rennet is：The permeability and permeability of non-woven fabrics are all fine, can ensure in the non-woven fabrics pleat
The confession of nutriment and/or oxygen on the inner and outer wall of rennet, and the inwall of cell culture incubator 1 needed for adherent cell growth
Good satisfaction should can be obtained, survival rate of the cell in incubation has obtained significant raising.
The preferred polyester fiber non-woven fabric of non-woven fabrics plicated layer, because its cellular affinity is good, polyester fiber non-woven fabric
Thickness preferably 2~3mm, such thickness make the non-woven fabrics plicated layer structural stability, mechanical performance, permeability and
Gas permeability can be effectively ensured.The polyester fiber non-woven fabric is by a diameter of 20~30 μm of polyester fiber braiding
Form.The purpose for selecting polyester fiber in this is：Make the non-woven fabrics plicated layer structural stability, mechanical performance, infiltration
Property and gas permeability can be effectively ensured.
Preferably, in order to further improve the inwall of cell culture incubator 1, and the cell of the inwall of the non-woven fabrics plicated layer
Compatibility, is convenient for the adhere-wall culture of more many cells, the internal chamber wall of the cell culture incubator 1 and the non-woven fabrics plicated layer
Two sides is surface-treated by vacuum plasma.
Further specifically, in order to ensure the structural stability of the non-woven fabrics plicated layer, the cell culture incubator 1
The center of intracavity bottom is conformed with 17, by the non-woven fabrics plicated layer in the inner chamber of the cell culture incubator 1 it is radially fixed.
Optionally, the novel cell culture culture apparatus is also included by described emulsion tube and cell culture incubator 1
The connected oxygen supply component of air inlet；And the oxygen supply component includes oxygen supply tank 14 and is arranged at oxygen confession
Answer the oxygen flux instrument 15 for measure control oxygen supply amount between tank 14 and cell culture incubator 1.
Using it is above-mentioned according to desirable embodiment of the present utility model as enlightenment, pass through above-mentioned description, related work people
Member can carry out various changes and amendments in the range of without departing from this item utility model technological thought completely.This item is real
The content being not limited to new technical scope on specification, it is necessary to which its technology is determined according to right
Property scope.
Claims (10)
- A kind of 1. novel cell culture device, it is characterised in that one connected including cell culture incubator, with cell culture incubator inside Enter liquid adapter and one and go out liquid adapter, and the oxygen measuring device for being used to measure oxygen content being arranged in cell culture incubator；WhereinIt is described enter liquid adapter be connected with and flow into component for the liquid for controlling nutrient solution to enter, it is described go out liquid adapter be connected with and be used for Control the liquid discharge component of nutrient solution outflow；The liquid flows into component and liquid discharge component is electrically connected with a speed adjuster；The internal chamber wall of the cell culture incubator be fixed with annular cell attachment dielectric layer, the cell attachment dielectric layer with it is described interior Cavity wall separates；AndAn air inlet is additionally provided with the cell culture incubator, the air inlet by emulsion tube respectively with carbon dioxide provisioning component and Nitrogen supply (NS) component is connected；The air inlet is configured with the air intake valve for controlling air inlet off and on.
- 2. novel cell culture device according to claim 1, it is characterised in that the carbon dioxide provisioning component includes Carbon dioxide charging-tank and the measure control carbon dioxide that is used for being arranged between carbon dioxide charging-tank and cell culture incubator supply The carbon dioxide flow instrument that should be measured；AndThe nitrogen supply (NS) component include nitrogen supply (NS) tank and be arranged between nitrogen supply (NS) tank and cell culture incubator based on The nitrogen flow instrument of amount control nitrogen supply (NS) amount.
- 3. novel cell culture device according to claim 2, it is characterised in that be additionally provided with use in the cell culture incubator In the carbon dioxide meter of metering carbon dioxide content.
- 4. according to the novel cell culture device described in claim any one of 1-3, it is characterised in that be located on the emulsion tube The junction of emulsion tube and air inlet is provided with the sterilised membrane filter for aseptic filtration.
- 5. novel cell culture device according to claim 4, it is characterised in that the speed adjuster is provided with for inputting The touch display screen of control signal and display time flow.
- 6. novel cell culture device according to claim 5, it is characterised in that the liquid flows into the liquid feeding end of component Connected by sterile medical fluid flow tube with fresh medium bottle；AndThe outlet end of the liquid discharge component is connected by sterile medical fluid flow tube with waste collection bottle.
- 7. novel cell culture device according to claim 6, it is characterised in that the cell attachment dielectric layer is nonwoven Cloth plicated layer.
- 8. novel cell culture device according to claim 7, it is characterised in that the novel cell culture culture apparatus Also include the oxygen supply component being connected by described emulsion tube with the air inlet of cell culture incubator；AndThe oxygen supply component include oxygen supply tank and be arranged between oxygen supply tank and cell culture incubator based on The oxygen flux instrument of amount control oxygen supply amount.
- 9. novel cell culture device according to claim 8, it is characterised in that the cell culture incubator, which is additionally provided with, to be used for The exhaust outlet of exhaust；The exhaust outlet is provided with the drain tap for the on and off for being used to control exhaust outlet.
- 10. novel cell culture device according to claim 9, it is characterised in that the interior bottom of chamber of the cell culture incubator The center in portion is conformed with, by the non-woven fabrics plicated layer in the inner chamber of the cell culture incubator it is radially fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720595639.8U CN206843509U (en) | 2017-05-26 | 2017-05-26 | A kind of novel cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720595639.8U CN206843509U (en) | 2017-05-26 | 2017-05-26 | A kind of novel cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206843509U true CN206843509U (en) | 2018-01-05 |
Family
ID=60797769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720595639.8U Expired - Fee Related CN206843509U (en) | 2017-05-26 | 2017-05-26 | A kind of novel cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206843509U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111218403A (en) * | 2019-03-12 | 2020-06-02 | 华道(上海)生物医药有限公司 | Totally enclosed cell culture method |
-
2017
- 2017-05-26 CN CN201720595639.8U patent/CN206843509U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111218403A (en) * | 2019-03-12 | 2020-06-02 | 华道(上海)生物医药有限公司 | Totally enclosed cell culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wu Xing Inventor after: Yan Litao Inventor after: Chu Jin Inventor after: Li Mingshu Inventor before: Yan Litao Inventor before: Chu Jin Inventor before: Li Mingshu |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180105 Address after: 200000 Jingan District Yanchang Middle Road, Jingan District, Shanghai City, No. tenth Affiliated Hospital of Tongji University Co-patentee after: Yan Litao Patentee after: Wu Xing Address before: No. 5, Xixia villa village merchant village, Xinxia villa, Changzhou City, Jiangsu Province Patentee before: Yan Litao |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180105 Termination date: 20200526 |