CN206814678U - A kind of device for extracting marine streptomyces natural blue pigment - Google Patents
A kind of device for extracting marine streptomyces natural blue pigment Download PDFInfo
- Publication number
- CN206814678U CN206814678U CN201720485748.4U CN201720485748U CN206814678U CN 206814678 U CN206814678 U CN 206814678U CN 201720485748 U CN201720485748 U CN 201720485748U CN 206814678 U CN206814678 U CN 206814678U
- Authority
- CN
- China
- Prior art keywords
- tank
- suction filtration
- suction
- blue pigment
- marine streptomyces
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000187747 Streptomyces Species 0.000 title claims abstract description 37
- 239000001055 blue pigment Substances 0.000 title claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000000967 suction filtration Methods 0.000 claims abstract description 45
- 238000000605 extraction Methods 0.000 claims abstract description 23
- 238000000746 purification Methods 0.000 claims abstract description 13
- 239000007921 spray Substances 0.000 claims abstract description 13
- 239000012528 membrane Substances 0.000 claims description 32
- 238000003756 stirring Methods 0.000 claims description 20
- 238000002347 injection Methods 0.000 claims description 13
- 239000007924 injection Substances 0.000 claims description 13
- 238000003860 storage Methods 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 abstract description 10
- 238000001914 filtration Methods 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000003672 processing method Methods 0.000 abstract description 2
- 241001655322 Streptomycetales Species 0.000 abstract 2
- 239000012530 fluid Substances 0.000 abstract 2
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 description 14
- 239000000049 pigment Substances 0.000 description 13
- 238000000855 fermentation Methods 0.000 description 8
- 230000004151 fermentation Effects 0.000 description 8
- 210000002421 cell wall Anatomy 0.000 description 7
- 239000002002 slurry Substances 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- RDFLLVCQYHQOBU-GPGGJFNDSA-O Cyanin Natural products O([C@H]1[C@H](O)[C@H](O)[C@H](O)[C@H](CO)O1)c1c(-c2cc(O)c(O)cc2)[o+]c2c(c(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O3)cc(O)c2)c1 RDFLLVCQYHQOBU-GPGGJFNDSA-O 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- RDFLLVCQYHQOBU-ZOTFFYTFSA-O cyanin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=[O+]C1=CC(O)=C2)C=3C=C(O)C(O)=CC=3)=CC1=C2O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 RDFLLVCQYHQOBU-ZOTFFYTFSA-O 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000001054 red pigment Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000001052 yellow pigment Substances 0.000 description 2
- 241000223678 Aureobasidium pullulans Species 0.000 description 1
- 241000448637 Duganella sp. B2 Species 0.000 description 1
- 241000191025 Rhodobacter Species 0.000 description 1
- 241000131970 Rhodospirillaceae Species 0.000 description 1
- 241000187180 Streptomyces sp. Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000001040 synthetic pigment Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
技术领域technical field
本实用新型涉及微生物色素领域，具体涉及一种提取海洋链霉菌天然蓝色素的装置。The utility model relates to the field of microbial pigments, in particular to a device for extracting natural blue pigments from marine streptomyces.
背景技术Background technique
色素被大量地广泛应用于食品、医药、化妆品、保健品等工业生产。随着人们生活水平的提高，色素的需求量急剧增大，而人工合成色素因不具营养价值并具有毒害作用终将无法占领色素市场。相较于动植物色素，微生物色素具有不受时间、空间和环境等因素影响的优点，可以利用发酵装置进行大规模生产。蓝色作为三原色之一就注定了蓝色素的应用前景和潜力是巨大的，蓝色素可以搭配红，黄色素调制多种色调色素。Pigments are widely used in food, medicine, cosmetics, health products and other industrial production. With the improvement of people's living standards, the demand for pigments has increased sharply, and synthetic pigments will not be able to occupy the pigment market because they have no nutritional value and have toxic effects. Compared with animal and plant pigments, microbial pigments have the advantages of not being affected by factors such as time, space and environment, and can be produced on a large scale using fermentation equipment. As one of the three primary colors, blue is doomed to have a huge application prospect and potential. Blue pigment can be matched with red and yellow pigment to modulate various hues.
微生物色素作为一种天然色素因其安全、着色天然、无毒副作用等诸多优点，在国际市场销售额年增长率保持在10％以上。目前，食品应用的色素主要是红、黄色素，蓝色素比较稀缺，为解决这个问题，国外从六十年代开始致力于研究开发天然色素。其中，微生物蓝色素就是一个科研工作者的研究热点，现已鉴定和分离出多种能够合成蓝色素的微生物，包括链霉菌-ZLT(Streptomyces sp.)、假交替单胞菌(Pseudoaltermonas sp.E18)、杜檊式菌B2菌株(Duganella sp.B2)、出芽短梗霉(Aureobasidium pullulans)、红杆菌属(Rhodobacter)和紫色非硫细菌属等。As a natural pigment, microbial pigment has many advantages such as safety, natural coloring, and no side effects. The annual growth rate of sales in the international market has remained above 10%. At present, the pigments used in food are mainly red and yellow pigments, and blue pigments are relatively scarce. In order to solve this problem, foreign countries have devoted themselves to the research and development of natural pigments since the 1960s. Among them, microbial blue pigment is a research hotspot for scientific researchers. A variety of microorganisms capable of synthesizing blue pigment have been identified and isolated, including Streptomyces-ZLT (Streptomyces sp.), Pseudoaltermonas sp.E18 ), Duganella sp.B2, Aureobasidium pullulans, Rhodobacter and purple nonsulfur bacteria.
在行业需求上，微生物发酵法制备蓝色素可以很好的解决色素品种少、来源相对不足和成本高的问题。但是，目前的蓝色素工业化大生产还存在着生产工艺不完善，能耗大，劳动生产效率低下，产率和纯度以及品质不够高等诸多问题，所以急需从新工艺、新装置及高效菌种筛选驯化等几个方面来解决这些问题，从而达到增产增收的经济效益。In terms of industry needs, the preparation of blue pigment by microbial fermentation can well solve the problems of few pigment varieties, relatively insufficient sources and high cost. However, there are still many problems in the current industrialized production of blue pigment, such as imperfect production process, high energy consumption, low labor production efficiency, insufficient yield, purity and quality, so it is urgent to screen and domesticate from new processes, new devices and efficient strains. Wait for several aspects to solve these problems, so as to achieve the economic benefits of increasing production and income.
实用新型内容Utility model content
针对现有的蓝色素工业化大生产的生产工艺不完善，能耗大，劳动生产效率低下，产率和纯度以及品质不够高等诸多问题，本实用新型提供了一种提取海洋链霉菌天然蓝色素的装置，海洋链霉菌可同时产生胞外的发酵液和胞内的链霉菌两种不同的脂溶性和水溶性的蓝色素，本装置针对这两种不同的蓝色素对应采取了不同的处理方法，利用该类蓝色素在不同的pH值下其凝聚形态发生改变的特点，对其进行提纯。Aiming at many problems such as imperfect production process, high energy consumption, low labor production efficiency, insufficient yield, purity and quality of existing blue pigment industrialized large-scale production, the utility model provides a method for extracting natural blue pigment from marine streptomyces device, marine Streptomyces can simultaneously produce two different fat-soluble and water-soluble blue pigments in the extracellular fermentation broth and intracellular Streptomyces. This device adopts different processing methods for these two different blue pigments. Utilizing the feature that the cyanoid pigment changes its aggregation form at different pH values, it is purified.
本实用新型采用以下的技术方案：The utility model adopts the following technical solutions:
一种提取海洋链霉菌天然蓝色素的装置，包括储液罐和控制器，所述储液罐的上端设有投料口，储液罐的下部设有喷淋装置，喷淋装置的下方设有抽滤装置，抽滤装置和喷淋装置间设有搅拌装置，搅拌装置的底端设有旋转叶轮，喷淋装置的喷淋电机与控制器电连接，搅拌装置的搅拌电机与控制器电连接。A device for extracting the natural blue pigment of marine streptomyces, comprising a liquid storage tank and a controller, the upper end of the liquid storage tank is provided with a feeding port, the lower part of the liquid storage tank is provided with a spray device, and the lower part of the spray device is provided with Suction filtration device, there is a stirring device between the suction filtration device and the spraying device, the bottom of the stirring device is provided with a rotating impeller, the spraying motor of the spraying device is electrically connected to the controller, and the stirring motor of the stirring device is electrically connected to the controller .
抽滤装置包括抽滤漏斗、抽滤膜和抽滤泵，抽滤漏斗为倒三角结构，抽滤膜设置在抽滤漏斗的倒三角的顶边上，搅拌装置的旋转叶轮与抽滤膜的上平面保持微接触，搅拌装置的旋转叶轮能够在抽滤膜的上方微接触抽滤膜的平面旋转转动，旋转叶轮用于防止抽滤膜阻塞并加快过滤，抽滤泵能够驱动抽滤膜上待滤物质向下运动，抽滤漏斗的下方设有粗液收集罐和超声波破碎罐，能通过抽滤膜的胞内的链霉菌在抽滤泵的驱动下经抽滤漏斗进入超声波破碎罐，不能通过抽滤膜的胞外的发酵液沿着抽滤漏斗的外沿进入粗液收集罐。The suction filtration device comprises a suction filtration funnel, a suction filtration membrane and a suction filtration pump, the suction filtration funnel is an inverted triangle structure, the suction filtration membrane is arranged on the top edge of the inverted triangle of the suction filtration funnel, the rotating impeller of the stirring device and the suction filtration membrane The upper plane maintains micro-contact, and the rotating impeller of the stirring device can rotate and rotate on the plane of the suction filtration membrane in micro-contact above the suction filtration membrane. The rotating impeller is used to prevent the suction filtration membrane from clogging and speed up the filtration. The suction filtration pump can drive the suction filtration membrane to The material to be filtered moves downward, and there is a crude liquid collection tank and an ultrasonic crushing tank under the suction filter funnel. The intracellular streptomyces that can pass through the suction filter membrane enter the ultrasonic crushing tank through the suction filter funnel driven by the suction filter pump. The extracellular fermentation liquid that cannot pass through the suction filter membrane enters the crude liquid collection tank along the outer edge of the suction filter funnel.
所述超声波破碎罐的下方连接有萃取罐，萃取罐的上端设有萃取液注射口，超声波破碎后得到破碎的细胞浆液，包括胞内的蓝色素和细胞壁残渣，通过萃取液注射口注入能萃取胞内蓝色素的萃取液，为将萃取液与破碎的细胞浆液充分接触混合，萃取罐内设有卧式搅拌器，萃取罐的下方连接有离心罐，离心罐能够将萃取有胞内的链霉菌的清液和细胞壁分离，离心罐的底端设有排料口，离心罐的侧面连接有真空抽滤泵，离心罐的工作方式可为可控的间歇式运作，真空抽滤泵的尾端与所述粗液收集罐连接，真空抽滤泵将萃取有蓝色素的清液抽送到粗液收集罐中。The bottom of the ultrasonic crushing tank is connected with an extraction tank, and the upper end of the extraction tank is provided with an extraction liquid injection port. After ultrasonic crushing, the broken cell slurry, including intracellular blue pigment and cell wall residues, is injected through the extraction liquid injection port to extract For the extract of intracellular blue pigment, in order to fully contact and mix the extract with the broken cell slurry, a horizontal stirrer is installed in the extraction tank, and a centrifuge tank is connected below the extraction tank, which can extract the intracellular chain The supernatant of the mold is separated from the cell wall. There is a discharge port at the bottom of the centrifuge tank, and a vacuum filter pump is connected to the side of the centrifuge tank. The working mode of the centrifuge tank can be controlled intermittent operation. The end is connected with the crude liquid collection tank, and the vacuum filter pump pumps the clear liquid extracted with blue pigment into the crude liquid collection tank.
所述粗液收集罐的下方连接有pH纯化收集罐，pH纯化收集罐的上部侧面设置有pH调节剂注射口，pH纯化收集罐的底端设置有出料口。The bottom of the crude liquid collection tank is connected with a pH purification collection tank, the upper side of the pH purification collection tank is provided with a pH regulator injection port, and the bottom end of the pH purification collection tank is provided with a discharge port.
优选地，所述控制器包括控制面板。Preferably, said controller comprises a control panel.
优选地，所述抽滤膜的孔径为0.22μm，可以将海洋链霉菌的胞外发酵液和胞内链霉菌两种不同的蓝色素分离。Preferably, the pore size of the suction filtration membrane is 0.22 μm, which can separate two different blue pigments from the extracellular fermentation liquid of Streptomyces marinum and Streptomyces intracellulare.
优选地，所述超声波破碎罐内设有监测控温装置，监测控温装置能够监测温度并控制温度，使温度控制在蓝色素的最佳活性状态。Preferably, the ultrasonic crushing tank is provided with a temperature monitoring and control device, which can monitor and control the temperature, so that the temperature can be controlled at the optimal activity state of the blue pigment.
本发明具有以下有益效果：The present invention has the following beneficial effects:
(1)针对胞内外都存在色素的问题，设计了抽滤装置、超声波破碎罐、萃取罐和离心罐，将海洋链霉菌的胞内外两种不同的蓝色素对应采取了不同的处理方法，使海洋链霉菌的蓝色素提取更充分。(1) Aiming at the problem of pigments both inside and outside the cells, a suction filtration device, an ultrasonic crushing tank, an extraction tank and a centrifuge tank were designed, and different treatment methods were adopted for the two different blue pigments inside and outside the cells of Streptomyces marinum, so that The blue pigment of marine Streptomyces was more fully extracted.
(2)为解决因细胞堆积而降低滤过效率的问题，设计了搅拌装置，搅拌装置可由控制面板设置其转速，搅拌装置可以将过滤膜上的细胞扫除，进入下一个装置，增大过滤速度并简化过滤操作。(2) In order to solve the problem of reducing filtration efficiency due to cell accumulation, a stirring device is designed. The stirring device can set its speed by the control panel. The stirring device can sweep away the cells on the filter membrane and enter the next device to increase the filtration speed. And simplify the filtering operation.
(3)根据海洋链霉菌的蓝色素在不同的pH值下其凝聚形态发生改变的特点，设计了pH纯化收集罐，可通过pH调节剂注射口注入pH调节剂来提取纯化蓝色素。(3) According to the characteristics of the cyanin of marine streptomyces changing its aggregation form at different pH values, a pH purification collection tank was designed, and the pH regulator could be injected through the injection port of the pH regulator to extract and purify the cyanin.
附图说明Description of drawings
图1为提取海洋链霉菌天然蓝色素的装置的结构示意图。Fig. 1 is a structural schematic diagram of a device for extracting natural blue pigment of marine Streptomyces.
其中，1为投料口，2为储液罐，3为喷淋装置，4为搅拌装置，5为控制面板，6为抽滤漏斗，7为抽滤膜，8为抽滤泵，9为超声波破碎罐，10为萃取罐，11为萃取液注射口，12为卧式搅拌器，13为离心罐，14为排料口，15为真空抽滤泵，16为粗液收集罐，17为pH纯化收集罐，18为pH调节剂注射口，19为出料口。Among them, 1 is the feeding port, 2 is the liquid storage tank, 3 is the spray device, 4 is the stirring device, 5 is the control panel, 6 is the suction filter funnel, 7 is the suction filter membrane, 8 is the suction filter pump, 9 is the ultrasonic wave Crushing tank, 10 is the extraction tank, 11 is the extraction liquid injection port, 12 is the horizontal agitator, 13 is the centrifugal tank, 14 is the discharge port, 15 is the vacuum filter pump, 16 is the crude liquid collection tank, 17 is the pH In the purification collection tank, 18 is the injection port of the pH regulator, and 19 is the discharge port.
具体实施方式detailed description
下面结合附图对本实用新型进行具体的说明：Below in conjunction with accompanying drawing, the utility model is specifically described:
结合图1，一种提取海洋链霉菌天然蓝色素的装置，包括储液罐2和控制器，储液罐2的上端设有投料口1，储液罐2的下部设有喷淋装置3，喷淋装置3的下方设有抽滤装置，抽滤装置和喷淋装置3间设有搅拌装置4，搅拌装置4的底端设有旋转叶轮，喷淋装置3的喷淋电机与控制器电连接，搅拌装置4的搅拌电机与控制器电连接。In conjunction with Fig. 1, a device for extracting the natural blue pigment of marine Streptomyces comprises a liquid storage tank 2 and a controller, the upper end of the liquid storage tank 2 is provided with a feeding port 1, and the lower part of the liquid storage tank 2 is provided with a spray device 3, The bottom of the spray device 3 is provided with a suction filter device, and a stirring device 4 is provided between the suction filter device and the spray device 3. The bottom end of the stirring device 4 is provided with a rotating impeller. The spray motor of the spray device 3 and the controller electric Connect, the stirring motor of the stirring device 4 is electrically connected with the controller.
抽滤装置包括抽滤漏斗6、抽滤膜7和抽滤泵8，抽滤漏斗6为倒三角结构，抽滤膜7设置在抽滤漏斗6的倒三角的顶边上，搅拌装置4的旋转叶轮与抽滤膜7的上平面保持微接触，搅拌装置4的旋转叶轮能够在抽滤膜7的上方微接触抽滤膜7的平面旋转转动，旋转叶轮能够防止抽滤膜7阻塞并加快过滤，抽滤泵8能够驱动抽滤膜7上待滤物质向下运动，抽滤漏斗6的下方设有粗液收集罐16和超声波破碎罐9，胞内的链霉菌通过抽滤漏斗6进入超声波破碎罐9，胞外的发酵液沿着抽滤漏斗6的外沿进入粗液收集罐16。The suction filtration device comprises a suction filtration funnel 6, a suction filtration membrane 7 and a suction filtration pump 8, the suction filtration funnel 6 is an inverted triangle structure, and the suction filtration membrane 7 is arranged on the top edge of the inverted triangle of the suction filtration funnel 6, and the stirring device 4 The rotating impeller keeps micro-contact with the upper plane of the suction filtration membrane 7, and the rotary impeller of the stirring device 4 can rotate on the plane of the micro-contact suction filtration membrane 7 above the suction filtration membrane 7, and the rotation impeller can prevent the suction filtration membrane 7 from blocking and accelerate Filtration, the suction filtration pump 8 can drive the material to be filtered on the suction filtration membrane 7 to move downward, and the bottom of the suction filtration funnel 6 is provided with a crude liquid collection tank 16 and an ultrasonic crushing tank 9, and the intracellular streptomyces enters through the suction filtration funnel 6 The tank 9 is ultrasonically broken, and the extracellular fermentation liquid enters the crude liquid collection tank 16 along the outer edge of the suction filter funnel 6 .
超声波破碎罐9的下方连接有萃取罐10，萃取罐10的上端设有萃取液注射口11，超声波破碎后得到破碎的细胞浆液，包括胞内的蓝色素和细胞壁残渣，通过萃取液注射口11注入能萃取胞内的链霉菌的萃取液，为将萃取液与破碎的细胞浆液充分接触混合，萃取罐10内设有卧式搅拌器12，萃取罐10的下方连接有离心罐13，离心罐13能够将萃取有胞内的链霉菌的清液和细胞壁分离，离心罐13的底端设有排料口14，离心罐13的侧面连接有真空抽滤泵15，离心罐13的工作方式为可控的间歇式运作，真空抽滤泵15的尾端与粗液收集罐16连接，真空抽滤泵15将萃取有胞内的蓝色素的清液抽送到粗液收集罐16中。The bottom of the ultrasonic crushing tank 9 is connected with an extraction tank 10, and the upper end of the extraction tank 10 is provided with an extraction liquid injection port 11. After ultrasonic crushing, the broken cell slurry, including intracellular blue pigment and cell wall residue, is passed through the extraction liquid injection port 11. Inject the extract that can extract the streptomyces in the cell, for the extract and the broken cell slurry are fully contacted and mixed, the extraction tank 10 is provided with a horizontal agitator 12, and the bottom of the extraction tank 10 is connected with a centrifuge tank 13, and the centrifuge tank 13 can extract the clear liquid and the cell wall of the streptomyces in the extraction cell, the bottom of the centrifuge tank 13 is provided with a discharge port 14, the side of the centrifuge tank 13 is connected with a vacuum filter pump 15, and the working mode of the centrifuge tank 13 is Controllable intermittent operation, the tail end of the vacuum filtration pump 15 is connected to the crude liquid collection tank 16, and the vacuum filtration pump 15 pumps the clear liquid extracted with the blue pigment in the cells into the crude liquid collection tank 16.
粗液收集罐16的下方连接有pH纯化收集罐17，pH纯化收集罐17的上部侧面设置有pH调节剂注射口18，pH纯化收集罐17的底端设置有出料口19。The bottom of the crude liquid collection tank 16 is connected with a pH purification collection tank 17 , the upper side of the pH purification collection tank 17 is provided with a pH regulator injection port 18 , and the bottom end of the pH purification collection tank 17 is provided with a discharge port 19 .
其中，控制器包括有控制面板，抽滤膜7的孔径为0.22μm，可以将海洋链霉菌的胞外发酵液和胞内链霉菌两种不同的蓝色素分离，在超声波破碎罐9内设有监测控温装置，监测控温装置能够监测温度并控制温度，使温度控制在蓝色素的最佳活性状态。Wherein, the controller includes a control panel, and the pore diameter of the suction filter membrane 7 is 0.22 μm, which can separate the extracellular fermentation liquid of Streptomyces marinum and the two different blue pigments of Streptomyces intracellulare, and the ultrasonic crushing tank 9 is equipped with The temperature monitoring device can monitor and control the temperature, so that the temperature can be controlled at the best active state of the blue pigment.
使用时，将海洋链霉菌经投料口1投入到储液罐2中；储液罐2下部的喷淋装置3将海洋链霉菌喷淋到抽滤装置的抽滤膜7上，搅拌装置4的旋转叶轮在抽滤膜7的上方推动海洋链霉菌微接触抽滤膜7的平面旋转转动，旋转叶轮能够加快过滤过程并防止抽滤膜7阻塞，海洋链霉菌中能够通过抽滤膜7的胞内的链霉菌经抽滤膜7进入超声波破碎罐9中，海洋链霉菌中不能够通过抽滤膜7的胞外发酵液沿着抽滤漏斗6的外沿进入粗液收集罐16；胞内的链霉菌在超声波破碎罐9中进行超声破碎，超声频率为300W，每超声十秒间隔十秒，持续二十分钟；超声破碎后的细胞浆液(胞内的链霉菌和细胞壁)被抽送到萃取罐10中，通过萃取液注射口11注入CCl4萃取液，卧式搅拌器12将细胞浆液与CCl4萃取液充分混合；完成萃取后，将超声破碎并加入萃取液后所得到的混合液推送到离心罐13中，离心转速12000r/min下将混合液进行离心处理，得到上层的萃取有胞内的链霉菌的清液和下层的细胞壁残渣；上层的萃取有胞内的链霉菌的清液被真空抽滤泵15抽到粗液收集罐16中，下层的细胞壁残渣经排料口14排出；粗液收集罐16中的液体抽送至pH纯化收集罐17中，通过pH调节剂注射口18投入浓度为2mol/L的盐酸溶液，在酸性下沉降的蓝色素经出料口19产出固态蓝色素。During use, the marine Streptomyces is dropped into the liquid storage tank 2 through the feeding port 1; The rotating impeller pushes the plane rotation of the marine Streptomyces micro-contact suction filtration membrane 7 on the top of the suction filtration membrane 7, the rotating impeller can speed up the filtration process and prevent the suction filtration membrane 7 from clogging, and the cells of the marine Streptomyces can pass through the suction filtration membrane 7. Streptomyces in the interior enters in the ultrasonic crushing tank 9 through the suction filter membrane 7, and the extracellular fermentation liquid that cannot pass through the suction filter membrane 7 in the marine streptomyces enters the crude liquid collection tank 16 along the outer edge of the suction filter funnel 6; The Streptomyces was ultrasonically crushed in the ultrasonic crushing tank 9, the ultrasonic frequency was 300W, every ten seconds of ultrasonic interval was 10 seconds, and continued for 20 minutes; the cell slurry (intracellular Streptomyces and cell wall) after ultrasonic crushing was pumped to extract In the tank 10, the CCl4 extract is injected through the extract injection port 11, and the horizontal agitator 12 fully mixes the cell slurry and the CCl4 extract; In the centrifuge tank 13, under the centrifugation speed of 12000r/min, the mixed solution is centrifuged to obtain the supernatant of the streptomyces in the upper layer and the cell wall residue of the lower layer; the supernatant of the streptomyces in the upper layer is extracted Pumped into the crude liquid collection tank 16 by the vacuum filtration pump 15, the cell wall residue in the lower layer is discharged through the discharge port 14; the liquid in the crude liquid collection tank 16 is pumped into the pH purification collection tank 17, and passed through the pH regulator injection port 18 Throw in hydrochloric acid solution with a concentration of 2 mol/L, and the blue pigment that settles under acidity will produce solid blue pigment through the discharge port 19 .
当然，上述说明并非是对本实用新型的限制，本实用新型也并不仅限于上述举例，本技术领域的技术人员在本实用新型的实质范围内所做出的变化、改型、添加或替换，也应属于本实用新型的保护范围。Of course, the above description is not a limitation of the present utility model, and the present utility model is not limited to the above-mentioned examples, and changes, modifications, additions or replacements made by those skilled in the art within the essential scope of the present utility model are also acceptable. Should belong to the protection scope of the utility model.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720485748.4U CN206814678U (en) | 2017-05-04 | 2017-05-04 | A kind of device for extracting marine streptomyces natural blue pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720485748.4U CN206814678U (en) | 2017-05-04 | 2017-05-04 | A kind of device for extracting marine streptomyces natural blue pigment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206814678U true CN206814678U (en) | 2017-12-29 |
Family
ID=60755132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720485748.4U Expired - Fee Related CN206814678U (en) | 2017-05-04 | 2017-05-04 | A kind of device for extracting marine streptomyces natural blue pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206814678U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118588A (en) * | 2017-05-04 | 2017-09-01 | 山东科技大学 | A kind of method and apparatus for extracting marine streptomyces natural blue pigment |
| CN108607234A (en) * | 2018-04-04 | 2018-10-02 | 俞潺潺 | A kind of traditional Chinese medicine extraction device |
-
2017
- 2017-05-04 CN CN201720485748.4U patent/CN206814678U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118588A (en) * | 2017-05-04 | 2017-09-01 | 山东科技大学 | A kind of method and apparatus for extracting marine streptomyces natural blue pigment |
| CN107118588B (en) * | 2017-05-04 | 2019-05-24 | 山东科技大学 | A kind of method and apparatus for extracting marine streptomyces natural blue pigment |
| CN108607234A (en) * | 2018-04-04 | 2018-10-02 | 俞潺潺 | A kind of traditional Chinese medicine extraction device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206814678U (en) | A kind of device for extracting marine streptomyces natural blue pigment | |
| CN112244296A (en) | Preparation method and equipment of acerola cherry extract | |
| CN209317196U (en) | A kind of tea grounds separator in tea polyphenols production extraction process | |
| CN206570257U (en) | A kind of Polysaccharide in Pleurotus eryngii extraction element | |
| CN107955084B (en) | Reaction tank deslagging and separating system special for extracting chlorella polysaccharide | |
| CN203569090U (en) | Reaction and separation device of enzymic-method cephalosporin products | |
| CN107827996B (en) | Production line and process for extracting green alga proteoglycan from fresh green alga | |
| CN204073564U (en) | A kind of modified batch attractive gel filter | |
| CN211497608U (en) | Multi-functional ginseng fruit enzymolysis tank | |
| CN206152482U (en) | Natural pond liquid filtration system | |
| CN209481523U (en) | A kind of vegetable protein separating extraction device | |
| CN107118588A (en) | A kind of method and apparatus for extracting marine streptomyces natural blue pigment | |
| CN203582856U (en) | Large-scale microalgae separating, collecting and drying equipment | |
| CN217745970U (en) | Plant extract separating device | |
| CN216155870U (en) | Device for extracting plant protein in okra fruits by bundle extraction method | |
| CN108714315A (en) | A kind of tea polyphenol extract device of collection crushing function | |
| CN214210724U (en) | Automatic rice washing batching machine | |
| CN213700038U (en) | Extraction element of traditional chinese medicine chemical composition | |
| CN207811633U (en) | A kind of retort dedicated for green algae polysaccharide extraction removes slag piece-rate system | |
| CN207933324U (en) | A kind of production line extracting green alga proteoglycan from fresh green alga | |
| CN210543613U (en) | Separation device is used in production of yeast extract | |
| CN204841174U (en) | A filter equipment for amino acid production | |
| CN111957080A (en) | L-tyrosine separation equipment and L-tyrosine separation method | |
| CN204661885U (en) | A kind of acetic acid decolorizing purification treating apparatus | |
| CN210522687U (en) | Carboxymethyl lysine draws uses raw materials preprocessing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171229 Termination date: 20180504 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |