CN206710306U - A kind of planktonic organism imaging detection device - Google Patents

Abstract

The utility model discloses an imaging detection device for plankton. The device includes: a first laser, a second laser, and a color camera. The light emitted by the first laser is divided into reference light and object light by a dichroic prism. The reference light enters the color camera, and the object light passes through the plankton imaging detection area. , into the color camera, the mutual interference between the reference light and the object light in the color camera; the light emitted by the second laser passes through the plankton imaging detection area, and then enters the color camera; the light emitted by the first laser and the light emitted by the second laser The colors of the emitted light are different. In one exposure, the color camera can simultaneously collect the reference light and object light irradiated to the color camera and the light emitted by the second laser that passes through the plankton imaging detection area. The plankton imaging detection device provided by the utility model can realize high-resolution in-situ imaging detection of plankton under the condition of relatively turbid water quality.

Description

A plankton imaging detection device

technical field

The utility model relates to the field of imaging detection of plankton in water bodies, in particular to an imaging detection device for plankton.

Background technique

At present, the detection method for plankton is generally to collect plankton by sampling net and bring it back to the laboratory for detection, but this will cause some inevitable damage to plankton. There are also water sample collectors used to bring back water samples containing plankton to the laboratory for testing, but the sampling workload is particularly heavy. At present, general underwater cameras can only be used for imaging large-scale aquatic organisms because they do not have a microscopic imaging function. For plankton with a body size of less than 1mm, microscopic magnification is generally required for imaging detection, but the depth of field of the microscope is very small, so it is not suitable for imaging detection of plankton swimming in the 3D space of the water body.

At present, the existing technology is to use on-axis digital holography or off-axis digital holography to image and detect plankton. However, coaxial digital holography is only suitable for the imaging of sparse objects on a transparent background, and cannot be applied when the water quality is relatively turbid. Off-axis digital holography can separate the reconstructed image of the object from the conjugate image, but requires a large distance between the object to be measured and the image sensor. Without an optical magnifying lens, the numerical aperture of the system is small, resulting in imaging resolution If the optical magnification lens is added, the imaging field of view will be smaller. It can be seen that the prior art cannot solve the problem of high-resolution in-situ imaging of plankton under the condition of relatively turbid water quality.

Utility model content

The purpose of the utility model is to provide a plankton imaging detection device, which can realize high-resolution in-situ imaging detection of plankton under the condition of relatively turbid water quality.

In order to achieve the above object, the utility model provides the following scheme:

A plankton imaging detection device, the device includes: a first laser, a second laser, a color camera, the light emitted by the first laser is divided into reference light and object light by a beam splitting prism, and the reference light enters the In the color camera, the object light enters the color camera after passing through the plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; the light emitted by the second laser After passing through the plankton imaging detection area, it enters the color camera; the light emitted by the first laser is different from the light emitted by the second laser, and the color camera can, in one exposure, At the same time, the reference light irradiated to the color camera, the object light and the light emitted by the second laser passing through the plankton imaging detection area are collected.

Optionally, the plankton imaging detection device is wrapped with a sealed shell, and the sealed shell is provided with a first optical window and a second optical window.

Optionally, the plankton imaging detection device further includes: a first optical fiber, a second optical fiber, a first collimating lens, a second collimating lens, a first dichroic prism, a second dichroic prism, a third dichroic prism, a A reflecting mirror and a second reflecting mirror; the light emitted by the first laser is output through the first optical fiber, then becomes parallel light after passing through the first collimating lens, and then is divided into two beams by the first dichroic prism, Wherein, one beam is the reference light, and the other beam is the object light, the reference light is reflected by the first mirror, and then reflected by the second dichroic prism onto the image sensor of the color camera , the object light is reflected by the third dichroic prism and enters the plankton imaging detection area outside the sealed shell through the first optical window, and then enters the sealed shell through the second optical window irradiates the image sensor of the color camera through the second dichroic prism, and interferes with the reference light; the light emitted by the second laser is output through the second optical fiber, then passes through the After the second collimating lens becomes parallel light, after being reflected by the second reflector, it passes through the third dichroic prism, and then passes through the first optical window and enters the plankton outside the sealed shell for imaging The detection area enters the sealed shell through the second optical window, and finally shines on the image sensor of the color camera through the second dichroic prism, and the color camera can simultaneously capture The reference light, the object light and the light emitted by the second laser that pass through the plankton imaging detection area are irradiated to the color camera.

Optionally, the plankton imaging detection device further includes a controller, the controller is used to control the brightness of the first laser and the second laser and the action of the color camera.

Optionally, the plankton imaging detection device further includes a battery power supply, and the battery power supply is used to supply power to the first laser, the second laser and the color camera.

Optionally, the plankton imaging detection device further includes a control monitor, and the control monitor is respectively connected to the controller and the color camera through cables.

Optionally, the control monitor includes a monitor and a central processing unit, the monitor is used to display the image acquired by the color camera and the parameters of the color camera, the first laser and the second laser; the central processing The device is used to set the parameters of the first laser and the second laser, and control the color camera to take holographic pictures or videos through the controller, and complete the plankton imaging and detection of plankton in the area according to the holographic pictures or videos of imaging.

Optionally, the control monitor is also provided with a charging interface and an image and video export interface, the charging interface is used to charge the power battery, and the image and video export interface is used to export pictures or videos taken by the color camera .

According to the specific embodiment provided by the utility model, the utility model discloses the following technical effects: the plankton imaging detection device provided by the utility model adopts the dual-wavelength coaxial off-axis composite digital holographic technology to realize the original plankton in the water body. Two-dimensional imaging detection, using two different wavelength laser light sources to form off-axis digital holograms and coaxial digital holograms respectively, using a single exposure (one exposure) of a color camera to simultaneously record off-axis digital holograms and coaxial digital holograms, According to the off-axis digital hologram and on-axis digital hologram recorded simultaneously, the high-resolution imaging of plankton under turbid water quality is completed.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only the present invention. For some embodiments of the invention, those skilled in the art can also obtain other drawings according to these drawings without paying creative efforts.

Fig. 1 is the schematic structural diagram of the underwater plankton imaging detection device of the embodiment of the present invention;

Fig. 2 is a structural schematic diagram of the control monitor of the embodiment of the utility model.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

The purpose of the utility model is to provide a plankton imaging detection device, which can realize high-resolution in-situ imaging detection of plankton under the condition of relatively turbid water quality.

In order to make the above purpose, features and advantages of the utility model more obvious and understandable, the utility model will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

Fig. 1 is a schematic structural diagram of an underwater plankton imaging detection device according to an embodiment of the present invention. As shown in Fig. 1 , the plankton imaging detection device includes: a first laser 101, a second laser 112, a color camera 107, the first laser The light emitted by 101 is divided into reference light and object light by the dichroic prism, the reference light enters the color camera 107, and the object light enters the color camera 107 after passing through the plankton imaging detection area 110, and the The mutual interference between the reference light and the object light in the color camera; the light emitted by the second laser 112 enters the color camera 107 after passing through the plankton imaging detection area 110; the first laser The light emitted by 101 is different in color from the light emitted by the second laser 112 (two of the three colors of red, green, and blue), and the color camera 107 can simultaneously collect the irradiated light in one exposure. The reference light of the color camera, the object light and the light emitted by the second laser 112 after passing through the plankton imaging detection area 111 . The plankton imaging detection device is wrapped with a sealed shell 116 , and the sealed shell 116 is provided with a first optical window 109 and a second optical window 112 .

Specifically, after the light emitted by the first laser 101 is output through the first optical fiber 102, it becomes parallel light after passing through the first collimating lens 103, and then is divided into two beams by the first dichroic prism 104, wherein one beam is the reference light, the other beam is the object light, the reference light is reflected by the first reflector 105, and then reflected by the second dichroic prism 106 onto the image sensor of the color camera 107, After being reflected by the third dichroic prism 108, the object light enters the plankton imaging detection area 110 outside the sealed enclosure through the first optical window 109, and then enters the plankton imaging detection area 110 through the second optical window 111. In the above-mentioned sealed shell, and then pass through the second dichroic prism 106 to irradiate the image sensor of the color camera 107, and interfere with the reference light to form an off-axis digital hologram; the light emitted by the second laser 112 After being output by the second optical fiber 113, it becomes parallel light after passing through the second collimating lens 114, is reflected by the second reflector 115, passes through the third dichroic prism 108, and then passes through the The first optical window 109 enters the plankton imaging detection area 110 outside the sealed casing, enters the sealed casing through the second optical window 111, and finally shines on the plankton through the second dichroic prism 106. On the image sensor of the color camera 107, a coaxial digital hologram is formed; in one exposure, the color camera 107 can simultaneously collect the reference light, the object light and the second laser irradiated to the color camera The light emitted by 112 after passing through the plankton imaging detection area 110 .

The plankton imaging detection device also includes a controller 116, the controller 116 is used to control the brightness of the first laser 101 and the second laser 112 and the action of the color camera 107, the action includes: such as controlling The color camera 107 exposes and shoots holograms, videos, and the like.

The plankton imaging detection device also includes a battery power supply 117, and the battery power supply 117 is used to supply power to the first laser 101, the second laser 112 and the color 107 camera.

The plankton imaging detection device also includes a control monitor 119 , and the control monitor 119 is respectively connected to the controller 117 and the color camera 107 through cables 120 .

Fig. 2 is the structure schematic diagram of control monitor of the utility model embodiment, as shown in Fig. 2, described control monitor 119 comprises monitor 201 and central processing unit, and described monitor 201 is used for displaying that described color camera 107 acquires image and the parameters of the color phase 107, the first laser 101, and the second laser 112; the central processing unit is used to set the parameters of the first laser 101, the second laser 112 and control the The color camera 107 takes a hologram or a video, and completes the imaging of the plankton in the plankton imaging detection area 110 according to the hologram or video.

The control monitor 119 is also provided with an adjustment keyboard 203, which can be used to set the parameters of the color camera 107 and the laser. The picture record button 202 and the video record button 204 are used to operate and shoot a hologram or a hologram video. The control monitor 119 is also provided with a charging interface and an image and video export interface, the charging interface is used for charging the power battery 118 , and the image and video export interface is used for exporting pictures or videos taken by the color camera 107 .

When in use, use the load-bearing cable to put the underwater plankton imaging detection device into the water, record the digital hologram or digital holographic video by controlling the monitor operation, import it into the computer and use the dual-wavelength coaxial off-axis composite digital holographic reproduction algorithm through the reproduction program For hologram or video reproduction, the reproduction distance can be changed automatically from Z1 to Z2 in the program (Z1 is the distance between the color camera image sensor and the optical window 2, Z2 is the distance between the color camera image sensor and the optical window 1 ), the imaging detection of plankton in the imaging detection area can be realized.

The utility model is based on the dual-wavelength coaxial off-axis composite digital holographic technology, and uses the low-precision phase distribution φoff of the object light wave in the image sensor plane (Recording plane) reconstructed from the off-axis digital hologram (Ioff) as the coaxial digital hologram ( Iin) The initial phase distribution of phase recovery iterative reconstruction, adding the low-precision phase distribution 00-off of the object light wave reconstructed from the off-axis digital hologram (Ioff) in the plane of the object to be measured (Object) as the coaxial digital hologram (Iin) Constraints for iterative reconstruction of phase recovery. Therefore, the utility model is suitable for imaging detection of plankton in the water body with a body size ranging from 10 microns to 1 mm when the water quality is relatively turbid or the concentration of plankton (such as algae) is high. Moreover, the utility model has higher resolution and larger field of view, and has the characteristics of high detection efficiency. Its resolution is about the same as the pixel size of a color camera image sensor, and the imaging field of view is equal to the photosensitive area of the image sensor. The volume of the water body that can be detected by taking a hologram is V=S*d, where S is the photosensitive area of the image sensor, and d is the distance between the optical window 1 and the optical window 2 .

Each embodiment in this specification is described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other. As for the system disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and for the related information, please refer to the description of the method part.

In this paper, specific examples have been used to illustrate the principle and implementation of the present utility model, and the description of the above embodiments is only used to help understand the method of the present utility model and its core idea; meanwhile, for those of ordinary skill in the art, according to Thoughts of the present utility model all have changes in specific implementation and scope of application. To sum up, the contents of this specification should not be understood as limiting the utility model.

Claims (8)

1. A plankton imaging detection device, characterized in that, said device comprises: a first laser, a second laser, a color camera, and the light emitted by said first laser is divided into reference light and object light by a spectroscopic prism, so The reference light enters the color camera, the object light enters the color camera after passing through the plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; The light emitted by the second laser enters the color camera after passing through the plankton imaging detection area; the light emitted by the first laser is different from the light emitted by the second laser, and the color camera In one exposure, the reference light irradiated to the color camera, the object light and the light emitted by the second laser passing through the plankton imaging detection area can be collected simultaneously. 2 . The plankton imaging detection device according to claim 1 , characterized in that, the plankton imaging detection device is wrapped with a sealed shell, and the sealed shell is provided with a first optical window and a second optical window. 3 . 3. The plankton imaging detection device according to claim 2, characterized in that, the plankton imaging detection device also comprises: a first optical fiber, a second optical fiber, a first collimating lens, a second collimating lens, a first A beam-splitting prism, a second beam-splitting prism, a third beam-splitting prism, a first reflector, and a second reflector; the light emitted by the first laser passes through the first optical fiber and then passes through the first collimating lens to become The parallel light is divided into two beams by the first dichroic prism, wherein, one beam is the reference beam, and the other beam is the object beam, and the reference beam is reflected by the first reflector, and then passed through the The second dichroic prism is reflected onto the image sensor of the color camera, and the object light passes through the first optical window after being reflected by the third dichroic prism and enters the plankton imaging detection outside the sealed casing. area, and then enter the sealed shell through the second optical window, and then irradiate the image sensor of the color camera through the second dichroic prism, and interfere with the reference light; the second laser After the emitted light is output through the second optical fiber, it becomes parallel light after passing through the second collimating lens, is reflected by the second reflector, passes through the third dichroic prism, and then passes through the first An optical window enters the plankton imaging detection area outside the sealed casing, enters the sealed casing through the second optical window, and finally illuminates the image of the color camera through the second dichroic prism On the sensor, in one exposure, the color camera can simultaneously collect the reference light irradiated to the color camera, the object light and the light emitted by the second laser passing through the plankton imaging detection area. Light. 4. The plankton imaging detection device according to claim 1, characterized in that, the plankton imaging detection device further comprises a controller, and the controller is used to control the brightness of the first laser and the second laser and the action of said color camera. 5. The plankton imaging detection device according to claim 1, characterized in that, the plankton imaging detection device also includes a battery power supply, and the battery power supply is used to supply the first laser, the second laser and The color camera is powered. 6. plankton imaging detection device according to claim 4, is characterized in that, described plankton imaging detection device also comprises control monitor, and described control monitor is respectively connected with described controller, described color camera by cable connected. 7. The plankton imaging detection device according to claim 6, wherein the control monitor comprises a monitor and a central processing unit, and the monitor is used to display the image obtained by the color camera and the color camera , the parameters of the first laser and the second laser; the central processing unit is used to set the parameters of the first laser and the second laser and control the color camera to take holographic pictures or videos through the controller, and according to the The holographic picture or video completes the imaging of the plankton in the plankton imaging detection area. 8. The plankton imaging detection device according to claim 6, wherein the control monitor is also provided with a charging interface and an image and video export interface, the charging interface is used to charge the power battery, and the image The video export interface is used to export pictures or videos taken by the color camera.