CN206385176U - Suspending cell culture bottle - Google Patents
Suspending cell culture bottle Download PDFInfo
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- CN206385176U CN206385176U CN201720037178.2U CN201720037178U CN206385176U CN 206385176 U CN206385176 U CN 206385176U CN 201720037178 U CN201720037178 U CN 201720037178U CN 206385176 U CN206385176 U CN 206385176U
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- bottle
- lid
- bottle lid
- cell culture
- suspending cell
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Abstract
The utility model discloses a kind of suspending cell culture bottle, including bottle, bottle lid and bottom of bottle lid, the size of bottom of bottle lid is more than the size of bottle lid, and bottle lid and bottom of bottle are covered and be provided with air-vent, and hydrophobic permeable membrane is provided with bottom of bottle lid.The suspending cell culture bottle that the utility model is provided, opening at two is provided with bottle, and bottle cap is equipped with respectively at two openings, for bottle lid and bottom of bottle lid, bottle lid and bottom of bottle, which are covered, is provided with air-vent, and is provided with hydrophobic permeable membrane in bottom of bottle lid, to avoid culture medium from spilling, in use, can effectively increase the exchange area of culture medium and air.In the case where vapor permeation area increases, the volume that culture medium can be accommodated in blake bottle is also increased a lot, therefore the replacing number of times of blake bottle is reduced to a certain extent, the risk of cell contamination when reducing conversion blake bottle, so as to improve the efficiency of cell culture.
Description
Technical field
The utility model is related to biological cell culture technique field, more specifically to a kind of suspending cell culture bottle.
Background technology
Cell culture technology refers to maintaining the technology of the growth of cell under in vitro conditions.Whether branch is invested according to cell
The characteristic grown on thing is held, is divided into attaching type, floating type.It is main using two kinds of sides of quiescent culture and dynamic cultivation in culture
Formula.Under conditions of quiescent culture, in order to ensure that cell can have the gaseous environment of abundance, to the body of the culture medium in blake bottle
Product has under individual requirement, general condition, and culture volume and blake bottle floor space ratio are 0.2ml/cm2~0.5ml/cm2.Exceed
After this ratio, it is necessary to which rolling bottle or stir culture bottle carry out inflating to meet cell to gas under dynamic cultivation or liquid level
The need for.
Existing Tissue Culture Flask is made up of bottle and bottle cap, the open top of bottle, is tightened by bottle cap, and logical
Normal bottle cap has gas permeability.However, in the case where only bottle cap has gas permeability, the area with air exchange is smaller, limitation
During cell culture, the volume of the culture medium added in blake bottle so that need often to change blake bottle in incubation increases
Cause the risk of cell contamination.
In summary, how a kind of suspending cell culture bottle is provided, to increase the area with air exchange, blake bottle is reduced
Replacing number of times, improve cell culture efficiency, be current those skilled in the art's urgent problem to be solved.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of suspending cell culture bottle, to increase and air exchange
Area, reduces the replacing number of times of blake bottle, improves the efficiency of cell culture.
In order to achieve the above object, the utility model provides following technical scheme：
A kind of suspending cell culture bottle, including bottle, bottle lid and bottom of bottle lid, the size of the bottom of bottle lid are more than the bottle
The size of lid, and the bottle lid and the bottom of bottle cover and be provided with air-vent, is provided with the bottom of bottle lid hydrophobic
Air film.
It is preferred that, the bottle is cylindric bottle.
It is preferred that, the bottle is transparent polystyrene bottle.
It is preferred that, the hydrophobic permeable membrane is poly tetrafluoroethylene or polyvinylidene fluoride film.
It is preferred that, the hydrophobic permeable membrane is provided with the bottle lid.
It is preferred that, external screw thread, the bottle lid and the bottom of bottle lid are provided with the bottle mouth position and bottom of bottle of the bottle
Inside outer wall be provided with internal thread.
It is preferred that, a diameter of 10mm~50mm of the bottle lid, a diameter of 50mm~200mm of the bottom of bottle lid, institute
The height for stating bottle is 50mm~500mm.
It is preferred that, it is respectively arranged between the bottle lid and the bottle, between the bottom of bottle lid and the bottle close
Sealing.
It is provided with opening at two, and two openings and distinguishes on the suspending cell culture bottle that the utility model is provided, bottle
Bottle cap is equipped with, is bottle lid and bottom of bottle lid, bottle lid and bottom of bottle, which are covered, is provided with air-vent, and is set in bottom of bottle lid
There is hydrophobic permeable membrane, to avoid culture medium from spilling, in use, can effectively increase the exchange area of culture medium and air.
In the case that vapor permeation area increases, the volume that culture medium can be accommodated in blake bottle is also increased a lot, therefore to a certain degree
On reduce the replacing number of times of blake bottle, reduce conversion blake bottle when cell contamination risk, so as to improve cell culture
Efficiency.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the accompanying drawing used required in description of the prior art is briefly described, it should be apparent that, drawings in the following description are only
It is embodiment of the present utility model, for those of ordinary skill in the art, on the premise of not paying creative work, also
Other accompanying drawings can be obtained according to the accompanying drawing of offer.
The structural representation for the suspending cell culture bottle that Fig. 1 provides for the utility model embodiment；
The explosive view for the suspending cell culture bottle that Fig. 2 provides for the utility model embodiment.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belongs to the scope of the utility model protection.
Fig. 1 to Fig. 2 is referred to, the suspending cell culture bottle provided for the utility model embodiment, including bottle 1, bottleneck
Lid 2 and bottom of bottle lid 3, the size of bottom of bottle lid 3 are more than the size of bottle lid 2, and are provided with bottle lid 2 and bottom of bottle lid 3 ventilative
Hydrophobic permeable membrane 5 is provided with hole 4, bottom of bottle lid 3.Due to being provided with air-vent 4 on bottle lid 2 and bottom of bottle lid 3, therefore significantly
The exchange area of air and culture medium is increased, so that the culture medium that can be accommodated in same volume blake bottle is more, is subtracted
Few number of times for needing to change blake bottle in cell cultivation process.
In the prior art, the blake bottle used due to quiescent culture and dynamic cultivation is inconsistent, when needing in two kinds of cultures
When being changed between mode, then need to change blake bottle, can also increase the risk of cell contamination.The preferred scheme of the application
In, above-mentioned bottle 1 is set to cylindric bottle, the blake bottle is can be applied not only to quiescent culture, additionally it is possible to for dynamic
Culture, if if dynamic cultivation, dynamic cultivation directly can be carried out using devices such as cell roller bottle machines, now, due to
Vapor permeation area increases, therefore, it is possible to reduce the step of being inflated under liquid level.
Specifically, bottle 1 is transparent polystyrene bottle, in order to observe situation in bottle.In addition, can also be on bottle 1
Graduation mark is set, to control the amount that culture medium is added.
As preferred, above-mentioned hydrophobic permeable membrane 5 is poly tetrafluoroethylene or polyvinylidene fluoride film.
When setting hydrophobic permeable membrane 5, in addition to being set in bottom of bottle lid 3, it can also be set in bottle lid 2.Now, when
When blake bottle is in the state fallen, culture medium will not also overflow from the air-vent 4 on bottle lid 2.
As preferred, bottle cap is tightened with bottle 1 in the form of screw thread cooperation in the application.Specifically, bottle 1
Bottle mouth position and bottom of bottle at be provided with external screw thread, the inside outer wall of bottle lid 2 and bottom of bottle lid 3 is provided with internal thread.
In addition, it is contemplated that the sealing between bottle cap and bottle 1, can also between bottle lid 2 and bottle 1, bottom of bottle lid 3 with
Seal is set respectively between bottle 1.Specifically, on the one hand above-mentioned seal can play sealed effect, on the other hand
Effect with waterproof.
Blake bottle in the application, in the case where it is cylindrical-shaped structure, the diameter by bottle lid 2 that can be preferably is set
10mm~50mm is set to, the diameter of bottom of bottle lid 3 is set to 50mm~200mm, and the height of bottle 1 is set to 50mm~500mm.It is right
The effect that can be reached in the blake bottle of the application, here using the floor space of bottom of bottle lid 2 as 50cm2Exemplified by illustrate, bottle cap
Vapor permeation area is usually 3cm2Left and right, like this, the blake bottle in the application cause vapor permeation area to increase at least 17.5 times,
17.5 times of nutrient solution can also equally be added without carrying out dynamic cultivation more.
Opening at two, and two openings are provided with the suspending cell culture bottle that the utility model embodiment is provided, bottle 1
Place is equipped with bottle cap respectively, is to be provided with air-vent 4 on bottle lid 2 and bottom of bottle lid 3, bottle lid 2 and bottom of bottle lid 3, and
Hydrophobic permeable membrane 5 is provided with bottom of bottle lid 3, to avoid culture medium from spilling, in use, can effectively increase culture medium and air
Exchange area.Vapor permeation area increase in the case of, can be accommodated in blake bottle culture medium volume also increase a lot, because
This reduces the replacing number of times of blake bottle to a certain extent, the risk of cell contamination when reducing conversion blake bottle, so as to carry
The high efficiency of cell culture.
It is described between the embodiment of each in this specification using progressive form, it is equal that each embodiment is stressed
Be similar portion between the difference with other embodiment, each embodiment mutually referring to.
The foregoing description of the disclosed embodiments, enables those skilled in the art to realize or use the utility model.
A variety of modifications to these embodiments will be apparent for a person skilled in the art, general original as defined herein
Reason can in other embodiments be realized in the case where not departing from spirit or scope of the present utility model.Therefore, this practicality is new
Type is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein and features of novelty phase
Consistent most wide scope.
Claims (8)
1. a kind of suspending cell culture bottle, it is characterised in that including bottle (1), bottle lid (2) and bottom of bottle lid (3), the bottom of bottle
The size for covering (3) is more than the size of the bottle lid (2), and is provided with the bottle lid (2) and the bottom of bottle lid (3)
Hydrophobic permeable membrane (5) is provided with stomata (4), the bottom of bottle lid (3).
2. suspending cell culture bottle according to claim 1, it is characterised in that the bottle (1) is cylindric bottle.
3. suspending cell culture bottle according to claim 2, it is characterised in that the bottle (1) is transparent polystyrene
Bottle.
4. suspending cell culture bottle according to claim 1, it is characterised in that the hydrophobic permeable membrane (5) is polytetrafluoro
Vinyl film or polyvinylidene fluoride film.
5. suspending cell culture bottle according to claim 1, it is characterised in that be provided with the bottle lid (2) described
Hydrophobic permeable membrane (5).
6. the suspending cell culture bottle according to any one of claim 1 to 5, it is characterised in that the bottleneck of the bottle (1)
External screw thread is provided with place and bottom of bottle, and the inside outer wall of the bottle lid (2) and the bottom of bottle lid (3) is provided with interior spiral shell
Line.
7. the suspending cell culture bottle according to any one of claim 1 to 5, it is characterised in that the bottle lid (2) it is straight
Footpath is 10mm~50mm, a diameter of 50mm~200mm of the bottom of bottle lid (3), the height of the bottle (1) for 50mm~
500mm。
8. the suspending cell culture bottle according to any one of claim 1 to 5, it is characterised in that the bottle lid (2) and institute
State between bottle (1), be respectively arranged with seal between the bottom of bottle lid (3) and the bottle (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720037178.2U CN206385176U (en) | 2017-01-12 | 2017-01-12 | Suspending cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720037178.2U CN206385176U (en) | 2017-01-12 | 2017-01-12 | Suspending cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206385176U true CN206385176U (en) | 2017-08-08 |
Family
ID=59492495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720037178.2U Active CN206385176U (en) | 2017-01-12 | 2017-01-12 | Suspending cell culture bottle |
Country Status (1)
| Country | Link |
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| CN (1) | CN206385176U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108344615A (en) * | 2018-01-24 | 2018-07-31 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems and control method |
| CN108359639A (en) * | 2018-01-24 | 2018-08-03 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems |
| CN109609379A (en) * | 2019-01-21 | 2019-04-12 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel bilateral vapor-permeable type culture bottle |
| CN109762740A (en) * | 2019-02-22 | 2019-05-17 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel hand-held bilateral vapor-permeable type culture bottle |
| CN109810899A (en) * | 2019-04-09 | 2019-05-28 | 苏州赛普生物科技有限公司 | Suspending cell culture bottle |
| CN110024754A (en) * | 2019-05-30 | 2019-07-19 | 黑龙江省农业科学院齐齐哈尔分院 | Callosobruchus chinensis supports worm and changes beans device |
| CN112410217A (en) * | 2020-11-06 | 2021-02-26 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal culture bottle |
-
2017
- 2017-01-12 CN CN201720037178.2U patent/CN206385176U/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108344615A (en) * | 2018-01-24 | 2018-07-31 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems and control method |
| CN108359639A (en) * | 2018-01-24 | 2018-08-03 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems |
| CN108344615B (en) * | 2018-01-24 | 2020-10-23 | 杭州安弼晟生物科技有限公司 | Circulating tumor cell capturing system and control method |
| CN109609379A (en) * | 2019-01-21 | 2019-04-12 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel bilateral vapor-permeable type culture bottle |
| CN109762740A (en) * | 2019-02-22 | 2019-05-17 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel hand-held bilateral vapor-permeable type culture bottle |
| CN109810899A (en) * | 2019-04-09 | 2019-05-28 | 苏州赛普生物科技有限公司 | Suspending cell culture bottle |
| CN110024754A (en) * | 2019-05-30 | 2019-07-19 | 黑龙江省农业科学院齐齐哈尔分院 | Callosobruchus chinensis supports worm and changes beans device |
| CN110024754B (en) * | 2019-05-30 | 2024-02-27 | 黑龙江省农业科学院齐齐哈尔分院 | Green bean weevil insect-raising and bean-changing device |
| CN112410217A (en) * | 2020-11-06 | 2021-02-26 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal culture bottle |
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