CN206223520U - A kind of 96 orifice plates of suitable protein precipitation - Google Patents

Abstract

The utility model relates to a 96-orifice plate suitable for precipitating proteins, which comprises a main pipe body, an opening is provided at the bottom of the main pipe body, and an auxiliary pipe chamber seamlessly connected with the main pipe body is arranged at the opening, so that the auxiliary pipe chamber is connected with the main pipe body The main body is connected and arranged, and an elastic sealing door is provided at the opening where the auxiliary tube warehouse communicates with the main body, so that the auxiliary tube warehouse and the main body are separated and arranged. When the liquid and protein particles in the main body are subjected to centrifugal force After being greater than the elasticity of the elastic sealing door, the elastic sealing door is arranged to open toward the inner wall of the auxiliary tube warehouse. In the utility model, the pipette is not easy to touch the precipitated particles during the pipetting process. In addition, the particles are separated in space from the liquid in the main body in the auxiliary tube chamber, and are hardly affected by the water flow generated by the siphon, and the particles are not easy to float. The suction volume is more accurate, the sample is cleaner, and it is suitable for analysis and detection.

Description

A 96-well plate suitable for precipitating proteins

technical field

The utility model relates to an orifice plate, in particular to a 96-orifice plate suitable for precipitating proteins.

Background technique

Protein precipitation is a commonly used sample pretreatment method for the analysis of drug concentration in plasma samples. Its purpose is to remove the protein in the plasma, extract the drug bound to the protein into an organic reagent, and finally achieve the purpose of purifying or concentrating the sample.

The specific process is as follows:

Adding samples: A certain volume of plasma is added to the extraction container.

Add protein precipitation agent: usually organic chemical reagents such as methanol, ethanol, acetonitrile, etc., the volume is greater than twice the plasma volume.

Vortex: Vortex or shake the extraction container to thoroughly mix the plasma and extraction reagents. Proteins in plasma are denatured and aggregated into solid particles under the action of organic reagents.

Centrifugation: After the protein is fully denatured, the extraction container is centrifuged, and the solid protein particles are separated from the extraction solution at the bottom of the container.

Separation: Finally, use a pipette to separate the extract above the protein particles for subsequent analysis and detection.

Currently, the extraction containers for protein precipitation mainly include small-volume centrifuge tubes or 96-well plates.

Commonly used 96-well plates have a liquid capacity of 1 or 2 mL per well. The shape inside each hole is a cube or a cylinder, and the bottom is U-shaped. The material of the 96-well plate is mainly polypropylene. When performing protein precipitation treatment, each 96-well plate can simultaneously extract 96 samples.

When the extract is separated after the precipitation of the protein, due to the structural characteristics of the 96-well plate, it is difficult to observe the boundary line between the bottom sediment and the extract in the middle part of the well. When using a pipette, the granular sediment at the bottom floats up under the action of the water flow generated by the siphon and enters the pipette. These particulate deposits can clog the pipette causing errors in the aspiration volume. Particles may also block the analytical instrument during the subsequent analysis process, making the instrument unusable or damaged.

In view of the above-mentioned defects, the designer, actively researches and innovates, in order to create a 96-well plate with a new structure suitable for precipitating proteins, so that it has more industrial application value.

Utility model content

In order to solve the above technical problems, the purpose of this utility model is to provide a 96-well plate suitable for precipitating proteins.

In order to achieve the above object, the utility model adopts the following technical solutions:

A 96-well plate suitable for precipitating proteins, including a main body, the bottom of the main body is provided with an opening, and the opening is provided with an auxiliary tube warehouse seamlessly connected with the main body, so that the auxiliary tube warehouse is connected with the main body Arrangement, an elastic sealing door is provided at the opening where the auxiliary tube warehouse communicates with the main body, so that the auxiliary tube warehouse is arranged separately from the main body. When the gravity of the protein in the main body is greater than the elasticity of the elastic sealing door, The elastic sealing door is arranged to open toward the inner wall of the auxiliary tube warehouse.

Further, the 96-well plate suitable for protein precipitation, wherein, the auxiliary tube chamber and the main body are integrally formed.

Still further, in the 96-well plate suitable for precipitating proteins, the opening of the elastic sealing door is in-line or cross-shaped.

Furthermore, in the 96-well plate suitable for protein precipitation, the material of the elastic sealing door is soft silica gel, or a polymer film.

Still further, in the 96-well plate suitable for precipitating proteins, the elastic sealing door is sleeved on the opening at the bottom of the main body, and when the main body is filled with liquid, the elastic sealing door will The body and the auxiliary pipe warehouse are arranged in a sealed partition.

Still further, the 96-well plate suitable for protein precipitation, wherein, the shape of the auxiliary tube chamber is arranged in a cylindrical or conical shape.

By means of the above scheme, the utility model has at least the following advantages:

In the utility model, the pipette is not easy to touch the precipitated particles during the pipetting process. In addition, the particles are separated in space from the liquid in the main body in the auxiliary tube chamber, and are hardly affected by the water flow generated by the siphon, and the particles are not easy to float. The aspiration volume is more accurate and parallel. The cleanliness of the sample is greatly improved, and there is almost no granular substance in the separated extract, which is not easy to block the analysis instrument.

The above description is only an overview of the technical solution of the utility model. In order to understand the technical means of the utility model more clearly and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiment of the utility model with accompanying drawings. Rear.

Description of drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following drawings will be briefly introduced in the embodiments. It should be understood that the following drawings only show some embodiments of the present invention. Therefore, it should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained according to these drawings without creative work.

Fig. 1 is the structural representation of the utility model;

Fig. 2 is a schematic view of the structure of the utility model after the elastic sealing door is opened during operation.

detailed description

Below in conjunction with accompanying drawing and embodiment, the specific embodiment of the utility model is described in further detail. The following examples are used to illustrate the utility model, but not to limit the scope of the utility model.

In order to enable those skilled in the art to better understand the solution of the utility model, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described implementation Example is only a part of embodiments of the present utility model, rather than all embodiments. The components of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of the present utility model.

Example

As shown in Figures 1 and 2, a 96-well plate suitable for precipitating proteins includes a main body 1, an opening is provided at the bottom of the main body 1, and an auxiliary pipe seamlessly connected with the main body 1 is provided at the opening. The pipe warehouse 2 is arranged so that the auxiliary pipe warehouse 2 is connected with the main pipe body 1, and an elastic sealing door 3 is arranged at the opening where the auxiliary pipe warehouse 2 communicates with the main pipe body 1, so that the auxiliary pipe warehouse 2 is connected with the main pipe chamber. The body 1 is arranged separately, and when the gravity of the protein in the main body 1 is greater than the elasticity of the elastic sealing door 3, the elastic sealing door 3 is opened toward the inner wall of the auxiliary tube chamber 2. By opening an opening at the bottom of the main body 1 and installing an auxiliary pipe compartment 2 on the lower end of the opening, and installing an elastic sealing compartment door 3 between the two, the protein can be effectively separated, and it is hardly affected by the water flow generated by the siphon , the particles are not easy to float, and the volume of liquid absorption is more accurate.

The auxiliary pipe warehouse 2 and the main pipe body 1 described in the utility model are integrally formed and arranged to ensure the sealing performance of the auxiliary pipe chamber 2 and the main pipe body 1 .

The opening of the elastic sealing door 3 of the utility model is in-line or cross-shaped, and any one can be used, as long as the elastic sealing door 3 can separate the protein particles from the liquid when in use. .

The material of elastic sealing door 3 described in the utility model is soft silica gel, or is polymer film, generally uses soft silica gel, and its service life is relatively long, can reduce its cost.

In the utility model, the elastic sealing door 3 is socketed on the opening at the bottom of the main pipe body 1. When the main pipe body 1 is filled with liquid, the elastic sealing door 3 separates and seals the main pipe body 1 and the auxiliary pipe warehouse 2. The partition arrangement facilitates the replacement of the elastic airtight door 3 and ensures its airtightness.

The shape of the auxiliary tube warehouse 2 in the utility model is arranged in a cylindrical or conical shape, which can be selected according to needs.

The working principle of the utility model is as follows:

During the process of sample addition, reagent addition and vortex flow in the pretreatment of precipitating protein, the elastic sealing chamber door 3 is in a closed state. While in centrifugation, the denatured protein particles and extract solution push open the elastic sealing door 3 under the action of centrifugal force and enter the auxiliary tube chamber 2 . The protein particles are concentrated in the narrow auxiliary tube compartment 2 at the bottom, and the pipette is not easy to suck the protein particles at the bottom when separating the extract.

Example 1

For the extraction of 100 microliters of plasma samples, select a 96-well plate with a capacity of 50 microliters of auxiliary tube compartment 2 for extraction. First add 100 microliters of plasma, then add 400 microliters of methanol, place the 96-well plate on a plate shaker and vortex for 5 minutes, the protein in the plasma sample is fully denatured by methanol to form particles. After centrifugation, about 20 microliters of protein particles enter the 50-microliter auxiliary tube chamber 2 through the elastic sealing chamber door 3, thereby creating a spatial separation from the extract in the container. When pipetting the extract, the sample in the pipette is clear and free of particles, which is suitable for subsequent analysis and testing.

Example 2

For the extraction of 300 microliters of plasma samples, select a 96-well plate with a capacity of 100 microliters of auxiliary tube compartment 2 for extraction, first add 300 microliters of plasma, then add 1 mL of methanol, and place the 96-well plate on a shaker for 5 minutes. Proteins in plasma samples are fully denatured by methanol to form particles. After centrifugation, about 60 microliters of the protein particle enters the 100 microliter auxiliary tube chamber 2 through the elastic sealing chamber door 3, so that it is spatially separated from the extract in the container. When pipetting the extract, the sample in the pipette is clear and free of particles, which is suitable for subsequent analysis and testing.

In the utility model, the pipette is not easy to touch the precipitated particles during the pipetting process. In addition, the particles are separated in space from the liquid in the main body in the auxiliary tube chamber, and are hardly affected by the water flow generated by the siphon, and the particles are not easy to float. The aspiration volume is more accurate and parallel. The cleanliness of the sample is greatly improved, and there is almost no protein granular substance in the separated extract, which is not easy to block the analysis instrument.

The above is only a preferred embodiment of the utility model, and is not intended to limit the utility model. It should be pointed out that for those of ordinary skill in the art, they can also do Several improvements and modifications are made, and these improvements and modifications should also be regarded as the protection scope of the present utility model.

Claims (6)

1. a kind of 96 orifice plates of suitable protein precipitation, it is characterised in that：Including main pipe (1), the bottom of the main pipe (1) is opened An opening is provided with, the auxiliary pipe storehouse (2) being seamlessly connected with main pipe (1) is provided with the opening, make auxiliary pipe storehouse (2) and main pipe (1) be connected arrangement, and elastic packing door is provided with the opening that the auxiliary pipe storehouse (2) is connected with the main pipe (1) (3) auxiliary pipe storehouse (2) and main pipe (1) spaced apart, are made, when the liquid in main pipe (1) and centrifugal force suffered by protein body are big In after the elasticity of elastic packing door (3), elastic packing door (3) opens arrangement to the inwall direction of auxiliary pipe storehouse (2).

2. 96 orifice plates of suitable protein precipitation according to claim 1, it is characterised in that：The auxiliary pipe storehouse (2) and supervisor Body (1) is formed in one arrangement.

3. 96 orifice plates of suitable protein precipitation according to claim 1, it is characterised in that：The elastic packing door (3) Opening be yi word pattern, or for cross.

4. 96 orifice plates of suitable protein precipitation according to claim 3, it is characterised in that：The elastic packing door (3) Material be soft silica gel, or be thin polymer film.

5. 96 orifice plates of suitable protein precipitation according to claim 4, it is characterised in that：The elastic packing door (3) It is socketed on the opening of the bottom of the main pipe (1), when main pipe (1) is equipped with liquid, elastic packing door (3) will be responsible for Body (1) separates with auxiliary pipe storehouse (2) exclusion seal to be arranged.

6. 96 orifice plates of suitable protein precipitation according to claim 1, it is characterised in that：The shape of the auxiliary pipe storehouse (2) In cylinder or cone arrangement.