CN206192892U - Lactoperoxidase's quantitative determination kit - Google Patents

Abstract

A kit for quantitative detection of lactoperoxidase, which relates to a kit for quantitative detection of peroxidase in raw milk and dairy products, to solve the problem of cumbersome and time-consuming detection steps for the existing detection of lactoperoxidase. The problem of large operating errors. The fixed plate 5 is arranged in the box body 6, and the first reagent bottle 1, the second reagent bottle 2, the test strip 3 and the colorimeter 4 are respectively arranged in the first bottle hole 6-1, the second bottle hole 6-2, In the groove 6-3 of the test strip and the groove 6-4 of the colorimeter, the first reagent bottle 1 and the second reagent bottle 2 are respectively equipped with lactoperoxidase standard and hydrogen peroxide solution, and are used for the fixed plate 5 Hard paper or plastic foam board is made, and box body 6 is made with hard paper or thin plastic board. The utility model is used for rapid detection of peroxidase content in raw milk and its products.

Description

Quantitative detection kit for lactoperoxidase

technical field

The utility model relates to a peroxidase detection kit, in particular to a rapid detection kit for peroxidase content in milk and its products.

Background technique

Lactoperoxidase is a natural antibacterial substance present in animal milk, which is widely used in milk and dairy products. In the processing of dairy products, lactoperoxidase is often used as one of the indicators to check whether the pasteurization is sufficient. In view of the important biological function and production guidance of lactoperoxidase, the determination of the content and activity of lactoperoxidase is of great significance for ensuring the quality of dairy products and prolonging the shelf life of dairy products.

There are many studies on the detection of lactoperoxidase at home and abroad. Existing detection methods rely on large-scale instruments to measure enzyme activity and enzyme concentration. The method has high accuracy and sensitivity, but it is not suitable for on-site detection due to the need for large-scale instruments. At present, in view of the instrumental detection method of lactoperoxidase, the sample needs to be subjected to complex pretreatment and the processing time is long, and online detection cannot be realized. Therefore, developing a key technology for rapid, sensitive and accurate detection of lactoperoxidase content for raw milk acceptance and dairy product processing has important theoretical research value and practical application significance.

Utility model content

The purpose of this utility model is to solve the existing problems in the analysis and test method of lactoperoxidase content, such as cumbersome operation, professional technicians are required to operate, or the cost is high, or online detection cannot be realized, etc., to provide a A rapid and convenient quantitative detection kit for lactoperoxidase.

The utility model comprises a first reagent bottle 1, a second reagent bottle 2, a reagent strip 3, a color measuring instrument 4, a fixed plate 5 and a box body 6, the fixed plate 5 is arranged in the box body 6, and the fixed plate 5 is respectively There are first bottle hole 6-1, second bottle hole 6-2, test strip groove 6-3 and colorimeter groove 6-4, the first reagent bottle 1 is set in the first bottle hole 6-1 Among them, the second reagent bottle 2 is set in the second bottle hole 6-2, the test strip 3 is set in the test strip groove 6-3, the colorimeter 4 is set in the colorimeter groove 6-4, Lactoperoxidase standard is housed in the first reagent bottle 1, and hydrogen peroxide solution is housed in the second reagent bottle 2.

The utility model has the advantages that: the lactoperoxidase and hydrogen peroxide solution are weighed and prepared by the manufacturer, and put into the first reagent bottle 1 and the second reagent bottle 2 successively respectively, and then the first reagent bottle 1. The second reagent bottle 2 is loaded into the box body 6. When it is necessary to detect the activity of peroxidase in the raw milk and its products, the operator can prepare the reagent according to the kit instructions, and follow the given The operating steps are used to detect and analyze the peroxidase content in raw milk and its products. The utility model simplifies many complicated steps in the experiment operation, can save the time of the experimenter, and has small operation errors.

Description of drawings

Fig. 1 is a schematic diagram of the overall structure of the utility model.

detailed description

Specific Embodiment 1: This embodiment is described in conjunction with FIG. 1. This embodiment includes a first reagent bottle 1, a second reagent bottle 2, a reagent strip 3, a color measuring instrument 4, a fixed plate 5 and a box body 6, and the fixed plate 5 is arranged in the box body 6, the first bottle hole 6-1, the second bottle hole 6-2, the test strip groove 6-3 and the color measuring instrument groove 6-4 are respectively arranged on the fixed plate 5, the second One reagent bottle 1 is set in the first bottle hole 6-1, the second reagent bottle 2 is set in the second bottle hole 6-2, the test strip 3 is set in the test strip groove 6-3, and the colorimeter 4 is set in the groove 6-4 of the colorimeter, the first reagent bottle 1 is equipped with a lactoperoxidase standard product, and the second reagent bottle 2 is equipped with a hydrogen peroxide solution. The fixed plate 5 is made of hard paper or plastic foam board, and the box body 6 is made of hard paper or thin plastic board. The fixed plate 5 and the box body 6 made of hard paper, thin plastic board or plastic foam board have the characteristics of light weight and convenient carrying.

Specific Embodiment 2: This embodiment is described with reference to FIG. 1 . The first reagent bottle 1 of this embodiment is a brown glass bottle. There is a lactoperoxidase standard in a sealed package inside the bottle, which is Reagent Ⅰ and stored at -20°C. Other components and connections are the same as those in the first embodiment.

Embodiment 3: This embodiment is described with reference to FIG. 1 . The second reagent bottle 2 of this embodiment is a brown plastic bottle. There is a hydrogen peroxide solution sealed in the bottle, which is reagent II and stored at room temperature. Other components and connections are the same as those in the first embodiment.

Embodiment 4: This embodiment is described with reference to FIG. 1 . The test strip 3 of this embodiment is made of medium-speed quantitative filter paper, sealed in vacuum packaging, and stored at 4°C. Other components and connections are the same as those in the first embodiment.

Embodiment 5: This embodiment is described with reference to FIG. 1 . The colorimeter 4 of this embodiment is a photoelectric colorimeter. The photoelectric colorimeter is mainly composed of three parts: mechanical system, photoelectric system and circuit system. Store at room temperature. Other components and connections are the same as those in the first embodiment.

The utility model using method:

1. Preparation of reagents:

Preparation of lactoperoxidase standard series solutions: take out reagent Ⅰ, dilute with 10mL deionized water to final concentrations of 0mg/mL, 1mg/mL, 2mg/mL, 5mg/mL, 10mg/mL, 15mg/mL, 20mg/mL, 25mg/mL peroxidase standard solution. Prepare just before use.

2. Processing of samples to be tested:

1. Processing of liquid samples:

Take the liquid sample and centrifuge it in a refrigerated centrifuge for 20 minutes (4000r/min, 1-5°C) to remove the precipitate, then dilute it with distilled water to an appropriate concentration, mix it well, and set it aside.

2. Processing of solid samples:

After pulverizing various types of solid samples, weigh 0.05 g of the sample, dilute to an appropriate concentration with distilled water, mix well, and set aside.

3. Detection steps:

When testing with test paper, first add 3 μL of hydrogen peroxide solution dropwise to the prepared test strip, and then add 3 μL of the milk sample to be tested dropwise. After reacting for 3 minutes, measure the reflectance value on a photoelectric colorimeter at a wavelength of 655 nm. According to the reflective value of the tested paper, the content of lactoperoxidase in the tested sample can be known by means of the standard curve.

Claims (5)

1. the immue quantitative detection reagent box of lactoperoxidase, it includes the first reagent bottle 1, the second reagent bottle 2, reagent strip 3, colour examining Instrument 4, fixed plate 5 and box body 6, it is characterised in that：The fixed plate 5 is arranged in box body 6, and first is respectively equipped with fixed plate 5 Bottle hole 6-1, second bottle of hole 6-2, test strips groove 6-3 and colour photometer groove 6-4, the first reagent bottle 1 are arranged on first bottle of hole 6-1 In, the second reagent bottle 2 is arranged in second bottle of hole 6-2, and test strips 3 are arranged in test strips groove 6-3, and colour photometer 4 is arranged on In colour photometer groove 6-4, lactoperoxidase standard items are housed in the first reagent bottle 1, hydrogen peroxide is housed in the second reagent bottle 2 Solution, fixed plate 5 is made of cardboard or plastic foam plate, and box body 6 is made of cardboard or plastic board.

2. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：First reagent bottle 1 is Brown Glass Brown glass bottles and jars only.

3. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：Second reagent bottle 2 is brown plastic bottle.

4. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：The test strips 3 are Middling speed quantitative filter paper article, is sealed using vacuum packaging.

5. the immue quantitative detection reagent box of lactoperoxidase according to claim 1, it is characterised in that：The colour photometer 4 is Photoelectric color measurement instrument.