CN206089666U - Adopt amazing organizational project dynamic cultivation appearance of dynamic mechanics - Google Patents

Abstract

The utility model relates to a tissue engineering dynamic culturing instrument adopting dynamic mechanical stimulation, which comprises one or more cultivating instrument units, and the cultivating instrument unit includes a box body, a driving motor, a controller, a rotating support, an attracting block, and a culturing bottle and a rotor; the culture bottle is placed above the box body, and the bottom of the bottle is facing the drive motor, and the rotor is placed in the culture bottle; at least one of the rotor and the attracting block is a magnetic material and the two attract each other; the controller is connected with the driving motor circuit, and the controller has a speed control module, which can control the driving motor to work according to the set speed. The incubator can not only achieve general continuous and constant shear force stimulation, but also can generate cyclically changing agitation speed through program setting to provide cyclic dynamic shear force stimulation for the engineered tissue to further improve the engineering tissue. cultivation effect.

Description

A dynamic culture instrument for tissue engineering using dynamic mechanical stimulation

technical field

The utility model relates to the field of biomedical tissue engineering, in particular to a tissue engineering dynamic cultivator adopting dynamic mechanical stimulation.

Background technique

Tissue engineering is an emerging discipline that combines cell biology and material science to construct tissues or organs in vitro or in vivo. Its basic principle is to obtain a small amount of living tissue from the body, use special enzymes or other methods to separate cells (also known as seed cells) from the tissue for culture and expansion in vitro, and then combine the expanded cells with good biological phase. Capacitive, degradable, and absorbable biomaterials (scaffolds) are mixed in a certain proportion to allow cells to adhere to the biomaterials (scaffolds) to form a cell-material complex; a tissue or organ disease in which the complex is implanted into the body As the biomaterials are gradually degraded and absorbed in the body, the implanted cells continue to proliferate and secrete extracellular matrix in the body, and finally form corresponding tissues or organs, so as to achieve the purpose of repairing trauma and reconstructing functions.

The development of tissue engineering over the years has enabled people to successfully obtain tissue engineered cartilage with various shapes and uses, but the traditional culture methods are mostly static culture methods. The engineering tissue cultivated by the static culture method has poor biomechanical properties, compressive properties, elastic modulus, etc., which are quite different from the tissue to be repaired, and the repair goal cannot be achieved. In order to improve the mechanical properties of engineered tissues, the dynamic culture method is mostly used at present, usually through the rotation of the culture medium caused by stirring, and then provides shear force stimulation for the engineered tissues, which can improve the biomechanical properties of the engineered tissues. In dynamic culture, it is usually necessary to use a dynamic incubator with a stirring function, which is placed in an incubator under a sterile environment for cultivation. At present, a constant rotating speed incubator is usually used to provide the engineered tissue in the incubator with Mechanical stimulation, due to the constant rotational speed, provides a constant shear force in this way. Although this kind of mechanical stimulation has a good promotion effect on the biomechanical properties of engineered tissue compared with static culture in the culture of engineered tissue, the effect still needs to be improved.

Utility model content

In order to solve the above-mentioned technical problems, the utility model provides a dynamic culture instrument for tissue engineering that adopts dynamic mechanical stimulation. This culture instrument can not only realize general continuous and constant shear force stimulation, but also can control different stirring speeds for all The cultivated engineered tissue provides the stimulation of cyclic dynamic shear force, which further improves the culture effect of the engineered tissue.

The technical solution of the utility model for solving the above-mentioned technical problems is as follows: a tissue engineering dynamic culturing instrument adopting dynamic mechanical stimulation, which is different from the prior art in that it includes one or more cultivating instrument units, and the cultivating instrument unit includes a box body , a drive motor, a controller, a rotating support, an attraction block, a culture bottle and a rotor; the driving motor is placed inside the box body, and its power output end is vertically upward; the rotating support and the driving motor The power output end is fixedly connected, the attraction block is placed on the rotating support; the culture bottle is placed above the box body, and the bottom of the bottle is facing the drive motor, and the rotor is placed on the In the culture bottle; at least one of the rotor and the attraction block is made of magnetic material, and the two attract each other; the controller is connected to the drive motor circuit, and the controller has a speed control module, which can The drive motor is controlled to work according to the set speed.

On the basis of the above technical solutions, the utility model can also be improved as follows.

Further, the controller can control the driving motor to increase from a minimum speed value to a maximum speed value, and then decrease from the maximum speed value to the minimum speed value, so as to work in a cycle of duty cycle; The controller also has a time control module, which can control the time of the working cycle of the driving motor.

Further, a control panel and a display screen are provided outside the box, and the control panel is provided with a switch button connected to the controller circuit, a time setting button, a speed range setting button, an execution button and a number key; Control the opening and closing of the system through the switch button; press the time setting button and then set the working cycle time by pressing the corresponding number key; press the speed setting button and then set the speed by pressing the corresponding number key The minimum speed value and the maximum speed value; after setting the time of the working cycle or the minimum speed value and the maximum speed value of the speed, the execution button can be used to confirm or delete or run; the display screen can display the current speed change range value in real time and the time of the set duty cycle.

Further, the controller is a single-chip microcomputer.

Further, the outer wall of the box body is provided with a battery slot with a built-in battery, and the battery provides electric energy for the controller.

Further, there are multiple incubator units, two or more connection holes are provided on the side of the box body, and the DC power socket is detachably installed in each connection hole, and each The DC power sockets are all connected to the controller circuit, and all the DC power sockets on the box body are connected to each other; The plugs at both ends of the wire are adapted to the DC power socket; the plugs at both ends of the connecting line are respectively connected to the DC power sockets on different incubator units.

Further, the attracting block is a button magnet, and the distance between the attracting block and the rotation axis of the power output end of the driving motor is adjustable. The button magnet is used to provide attraction to the rotor in the bottle, which is simple and reliable. At the same time, because the distance between the attraction block and the rotation axis of the drive motor is adjustable, the radius of gyration of the attraction block can be adjusted to adapt to culture bottles of different sizes. It can also meet more parameter requirements in the experiment.

Further, the rotating support is made of ferromagnetic material, the attracting block is adsorbed on the rotating supporting and fixed, the rotating supporting is provided with a groove, and the attracting block is placed in the groove and can move in a direction close to or away from the rotation axis of the power output end of the drive motor in the groove.

The beneficial effect of adopting the above-mentioned further solution is that a groove is provided on the rotating support, so that the attracting block can be stably placed on the rotating support, and the radius of gyration of the attracting block can be adjusted conveniently.

Further, a supporting seat is installed inside the box body, and the driving motor is fixed on the supporting seat.

The beneficial effect of adopting the above-mentioned further solution is that the stability of the driving motor can be ensured during operation by using the fixing function of the support base, and the use effect of the incubator can be improved.

Further, it also includes a power adapter with an output voltage of 12v DC, one end of the power adapter is connected to a 220v AC power supply, and the other end is connected to the DC power socket in any one of the incubator units.

The beneficial effect of adopting the above further solution is that, through a 12v DC power adapter, any incubator unit can be connected to supply power to all connected incubator units at the same time, which is safe, reliable, low in cost, convenient and practical.

Further, the box body is made of high temperature and high pressure resistant material, the box body includes an upper cover and a base, and the upper cover and the base are hinged on one side.

The beneficial effect of adopting the above-mentioned further scheme is that since the incubator needs to be placed before the sterile incubator, it must be ensured to be in a sterile state, and it needs to be disinfected, and the most commonly used disinfection method in the laboratory is high temperature and high pressure disinfection. , so the box body is made of high temperature and high pressure resistant material, which can be conveniently sterilized in the laboratory. At the same time, after the upper cover and the base are hinged, it can be opened and closed conveniently.

Further, the upper part of the box body is provided with an annular retaining ring that protrudes from the upper surface of the box body and is adapted to the outer diameter of the culture bottle, and the center of the retaining ring is located at the power output end of the driving motor. on the axis of rotation.

The beneficial effect of adopting the above-mentioned further solution is that since the culture bottle needs to be placed on the box body, in order to ensure that the center of the culture bottle is located on the rotation axis of the drive motor as much as possible, a The corresponding ring-shaped retaining ring protruding from the upper surface of the box helps the operator to accurately place the culture bottle. When placing it, it only needs to be placed in the retaining ring to ensure that the drive motor has been aligned. There is no need to find the center of the drive motor through the upper cover, and once the upper cover is not made of transparent material, it is more difficult to place it accurately and effectively.

The beneficial effect of the utility model is that each incubator unit can drive the rotating support to rotate through the driving motor placed in the box body, and then drive the attracting block placed on the rotating support to rotate, and because the culture bottle is placed in the box The body is directly above the driving motor, and there is a rotor in the bottle that can attract each other with the attraction block, so the rotor will rotate in the bottle with the rotation of the attraction block, and then drive the culture solution in the bottle to rotate, realizing the cultivation The engineered tissue in the liquid is stimulated by shear force, which improves the mechanical properties of the engineered tissue. Through the connection line and the DC power socket on each incubator unit, multiple incubator units can be connected in parallel according to needs, so that multiple incubator units can work at the same time.

The speed of the drive motor can be controlled through the controller, and the drive motor can be set to cycle within a certain speed range through the time setting button and the speed control button on the control panel to achieve dynamic mechanical stimulation and further promote the project The synthesis of tissue cell matrix makes the culture effect of engineered tissue better.

In addition, the whole device is simple in structure, low in cost, safe and reliable, and can meet the needs of multiple experimental groups, greatly improving applicability.

Description of drawings

Fig. 1 is the overall three-dimensional structural schematic diagram of a specific embodiment of a tissue engineering dynamic culture instrument that adopts dynamic mechanical stimulation provided by the utility model;

Fig. 2 is a schematic structural view of one of the incubator units in the specific embodiment shown in Fig. 1 after opening the upper cover;

Fig. 3 is a schematic structural view of one of the incubator units in the specific embodiment shown in Fig. 1 after opening the upper cover and removing the front panel;

Fig. 4 is a schematic diagram of the facade structure of one of the incubator units of the specific embodiment shown in Fig. 1;

Fig. 5 is a schematic structural view of one of the incubator units of the specific embodiment shown in Fig. 1;

Fig. 6 is a schematic structural view of one of the incubator units without a culture bottle in the specific embodiment shown in Fig. 1;

Fig. 7 is a schematic diagram of a specific implementation manner of the control panel.

In the accompanying drawings, the list of parts represented by each label is as follows:

Box body 1, driving motor 2, rotating support 3, suction block 4, culture bottle 5, rotor 6, battery 7, battery slot 71, support seat 8, controller 10, control panel 11, display screen 12, retaining ring 13 , DC power socket 14, connecting line 15, power adapter 16, groove 31, upper cover 101, base 102, connection hole 103, wire 151, plug 152, switch button 201, time setting button 202, speed lower limit setting button 203 , speed upper limit setting button 204 , confirm button 205 , cancel button 206 , run button 207 , and number keys 208 .

detailed description

The principles and features of the present utility model are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the utility model, and are not used to limit the scope of the utility model.

As shown in Figures 1 to 7, Figure 1 is a schematic diagram of the overall three-dimensional structure of a specific embodiment of a tissue engineering dynamic culture instrument using dynamic mechanical stimulation provided by the utility model; Figure 2 is the specific implementation shown in Figure 1 In the example, a schematic structural view of one of the incubator units after opening the upper cover; Fig. 3 is a schematic structural view of one of the incubator units in the specific embodiment shown in Fig. 1 after opening the upper cover and removing the front panel; Fig. 4 is a schematic diagram of the facade structure of one of the incubator units of the specific embodiment shown in FIG. 1; FIG. 5 is a schematic structural view of one of the incubator units of the specific embodiment shown in FIG. 1; FIG. 7 is a schematic diagram of a specific implementation of the control panel.

In a specific embodiment of a tissue engineering dynamic culture instrument that adopts dynamic mechanical stimulation provided by the utility model, it includes one or more culture instrument units, and the culture instrument unit includes a box body 1, a drive motor 2, a controller 10. Rotating support 3, suction block 4, culture bottle 5 and rotor 6; the drive motor 2 is placed inside the box body 1, and its power output end is vertically upward; the rotating support 3 and the The power output end of the drive motor 2 is fixedly connected, the attraction block 4 is placed on the rotating support 3; the culture bottle 5 is placed above the box body 1, and can be directly placed on the box body 1 Surface, also can utilize auxiliary support to be placed on the top of box body 1. The bottom of the bottle is facing the drive motor 2, and the rotor 6 is placed in the culture bottle 5; at least one of the rotor 6 and the attraction block 4 is made of magnetic material, and the two attract each other; The controller 10 is connected to the drive motor 2 circuit, and the controller 10 has a speed control module, which can control the drive motor 2 to work at a set speed. The size of the rotor can be determined according to the size of the actual culture bottle and the needs of the actual experiment, but it should not be too large, so that the relative culture space in the culture bottle is larger. In this way, the small rotor can be used to stir the liquid in the culture bottle, and the relatively large space available in the culture bottle can be used to cultivate tissues with specific shapes, sizes, and densities according to the needs of the experimenter.

The controller 10 can control the driving motor 2 to increase from a minimum speed value to a maximum speed value, and then decrease from the maximum speed value to the minimum speed value, so as to work cyclically as a duty cycle; The controller 10 also has a time control module, which can control the time of the working cycle of the driving motor 2 .

The outside of the box body 1 is provided with a control panel 11 and a display screen 12, and the control panel 11 is provided with a switch button connected to the controller 10 circuit, a time setting button, a speed range setting button, an execution button and Numeric keys; control the opening and closing of the system through the switch button; press the time setting button and then press the corresponding number keys to set the time of the working cycle; press the speed setting button and then press the corresponding number keys to set The minimum speed value and the maximum speed value of the fixed speed; after setting the time of the working cycle or the minimum speed value and the maximum speed value of the speed, it can be confirmed or deleted or run by the execution button; the display screen 12 can display the current speed in real time. The speed variation range value and the time of the duty cycle it sets. There are many ways to realize the controller 10 , the most common way is to use a single chip microcomputer to realize.

The outer wall of the box body 1 is provided with a battery slot 71 , which contains a battery 7 , and the battery 7 provides electric energy for the controller 10 .

There are multiple incubator units, and two or more connection holes 103 are provided on the side of the box body 1, and the DC power socket 14 is detachably installed in each connection hole 103, and each Each of the DC power sockets 14 is connected to the controller 10, and is connected to all the DC power sockets 14 on the same box body 1; the connection line 15 includes a wire 151 and is connected to the The plugs 152 at both ends of the wire 151 are compatible with the DC power socket 14; the plugs 152 at both ends of the connection line 15 are respectively connected to the DC power sockets 14 on different incubator units.

The attracting block 4 is a button magnet, and the distance between the attracting block 4 and the rotation axis of the driving motor 2 is adjustable.

The rotating support 3 is made of a ferromagnetic material, and the attracting block 4 is fixed by being adsorbed on the rotating supporting seat 3. A groove 31 is provided on the rotating supporting seat 3, and the attracting block 4 is placed on the rotating support 3. The groove 31 can be moved in a direction close to or away from the rotation axis in the groove 31 .

A supporting base 8 is installed inside the box body 1 , and the driving motor 2 is fixed on the supporting base 8 . The support seat 8 can be set as a plate, and is seated on the upper part of the base 102, so that the lower part of the base 2 has sufficient space for placing other components.

For the convenience of operation, the control panel 11 and the display screen 12 can be installed on the side wall of the base 102 .

It also includes a power adapter 16 with an output voltage of 12v DC. One end of the power adapter 16 is connected to a 220v AC power supply, and the other end is connected to the DC power socket 14 in any one of the incubator units.

The box body 1 is made of high temperature and high pressure resistant material. The box body 1 includes an upper cover 101 and a base 102 , and the upper cover 101 is hinged on the base 102 .

The upper part of the box body 1 is provided with an annular retaining ring 13 that protrudes from the upper surface of the box body 1 and is adapted to the outer diameter of the culture bottle 5, and the center of the retaining ring 13 is located on the drive motor. 2 on the axis of rotation.

In the specific embodiment provided by the utility model, as shown in Fig. 3 and Fig. 4, the battery slot 71 is arranged at the bottom of the box body 1, and the battery 7 can be loaded from the outside, and then covered by a cover. In this way, it can be realized that the device can not only work with an external power supply, but also can work with a built-in power supply. External AC power supply or battery power supply are two power supply modes of the incubator, one of which can be selected according to needs.

Through a 12v DC power adapter, any incubator unit can be connected to supply power to all connected incubator units at the same time, which is safe, reliable, low in cost, convenient and practical.

In a preferred embodiment, as shown in Figure 7, the control panel 11 is provided with switch button 201, time setting button 202, speed lower limit setting button 203, speed upper limit setting button 204, confirm button 205, cancel Button 206, run button 207, number keys 208 and display screen 12, each button on the control panel 11 is all connected with single-chip controller 10, wherein number key 208 is made up of ten number keys from 0 to 9.

For example, it is necessary to set the drive motor 2 at 20-60 rpm (the lower limit of speed is 20 rpm, and the upper limit is 60 rpm), and a work cycle of 10 minutes. This setting means that the speed of the drive motor needs to be After increasing from 20 rpm to 60 rpm, then reducing to 20 rpm, this cycle time is 10 minutes, and so on. During operation, first press the start key of the switch key to start the device, and now the display screen starts to display numbers, then press the time setting button 202, and now the number keys 208 can be used to set the time of a working cycle of the drive motor 2. Time, since the working cycle is 10 minutes, then press the "1" key and the "0" key in turn, if the input is wrong, you can press the cancel button 206 to cancel, and then input the correct number key, if the input is correct, then Confirm by confirming button 205; Then press lower speed limit setting button 203 again, press " 2 " and " 0 " key successively, press confirming button 205 again; Then press speed upper limit setting button 204 again, again Press the "6" and "0" keys in turn, and then press the confirmation button 205. After the input is correct, you can press the run button 207. At this time, the drive motor 2 starts to run according to the set speed and time. During the setting and running process, the display screen 12 always displays the current number, so as to prompt the operator to operate, so that the device has good interactivity.

In this specific embodiment, there are two connecting holes 103 on each incubator unit, and different incubator units can be connected in pairs by connecting wires 15 . The number of connection holes 103 on each incubator unit can also be three or more. When there are more than two, each incubator unit can be connected to multiple incubator units, which is also applicable.

In this specific embodiment, the box body 1 has a roughly rectangular parallelepiped structure, and the dimensions of length, width and height are 12x8x5cm. In the use of culture bottles, in most cases, two specifications of 100ml and 250ml are used, and the heights are 99mm and 138mm respectively. Therefore, in a 46x46x48cm incubator, at least two layers can be placed, and each layer can be placed in three rows and four columns, or twelve. A total of at least 24 incubators can be placed, which is enough to meet the needs of experimental groups.

Moreover, since each culture instrument unit is independently controlled, the required rotational speed can be set separately, so different rotational speeds can be used for multiple culture instruments at the same time, and different mechanical stimuli can be provided for cultivating different target tissues to meet the needs of multiple cultures. However, in the prior art, if four instruments are used to give different mechanical stimuli to different tissues, multiple instruments and multiple incubators are required, which will undoubtedly increase the cost and inefficiency; The stirring speed of each seat is the same, which cannot meet the needs of experimental grouping.

In addition, the box body can also be set in a cylindrical shape, so that the structure is more compact and the occupied volume is smaller. The box body can also be designed as other various shapes such as ellipse, polygon, etc. It should be understood that the various shapes of the box body all belong to the spirit and principles of the present utility model, and should be included in the protection scope of the present utility model .

In the tissue engineering dynamic culturing instrument provided by the utility model that adopts dynamic mechanical stimulation, a certain number of cultivating instrument units can be selected according to needs, and connected to each other through connecting wires 15, so that multiple cultivating instrument units can be simultaneously And work independently.

The above descriptions are only preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present utility model shall be included in this utility model. within the scope of protection of utility models.

Claims (12)

1. a tissue engineering dynamic culturing instrument that adopts dynamic mechanical stimulation, is characterized in that, comprises one or more cultivating instrument units, and described cultivating instrument unit comprises box body (1), drive motor (2), controller (10 ), rotating support (3), attracting block (4), culture bottle (5) and rotor (6); The driving motor (2) is placed inside the box body (1), and its power output end is vertically upward; The rotating support (3) is fixedly connected to the power output end of the drive motor (2), and the attraction block (4) is placed on the rotating support (3); The culture bottle (5) is placed above the box body (1), and the bottom of the bottle is facing the drive motor (2), and the rotor (6) is placed in the culture bottle (5); At least one of the rotor (6) and the attraction block (4) is made of magnetic material, and the two attract each other; The controller (10) is connected to the drive motor (2) circuit, and the controller (10) has a speed control module, which can control the drive motor (2) to work at a set speed. 2. A kind of tissue engineering dynamic culture instrument adopting dynamic mechanical stimulation according to claim 1, characterized in that, the controller (10) can control the drive motor (2) to increase from a minimum rotational speed value to A maximum speed value, and then reduce from the maximum speed value to the minimum speed value, as a working cycle cycle; The controller (10) also has a time control module, which can control the time of the working cycle of the driving motor (2). 3. a kind of tissue engineering dynamic culture instrument that adopts dynamic mechanical stimulation according to claim 2, is characterized in that, described box body (1) outside is provided with control panel (11) and display screen (12), and described The control panel (11) is provided with a switch button connected to the controller (10) circuit, a time setting button, a speed range setting button, an execution button and a number key; Control the system on and off through the switch button; Press the time setting button and then set the working cycle time by pressing the corresponding number key; Press the speed setting button and then set the minimum and maximum speed values by pressing the corresponding number keys; After setting the working cycle time or the minimum speed value and the maximum speed value of the speed, the execution button can be used to confirm or delete or run; The display screen (12) can display the current speed, the currently set speed variation range and the set working cycle time in real time. 4. A tissue engineering dynamic culturing instrument using dynamic mechanical stimulation according to any one of claims 1 to 3, characterized in that the controller (10) is a single-chip microcomputer. 5. A kind of tissue engineering dynamic culture instrument that adopts dynamic mechanical stimulation according to claim 4, is characterized in that, the outer wall of described box body (1) is provided with battery groove (71), and described battery groove (71 ) has a built-in battery (7), and the battery (7) provides electric energy for the controller (10). 6. A kind of tissue engineering dynamic culture instrument that adopts dynamic mechanical stimulation according to claim 4, is characterized in that, the culture instrument unit is a plurality of, and the side of described box body (1) is provided with two or two More than two connection holes (103), each of the connection holes (103) is detachably installed with a DC power socket (14), each of the DC power sockets (14) is connected to the controller (10) Circuit connection, and circuit connection between all DC power sockets (14) on the same box body (1); The incubator also includes a plurality of connecting wires (15), and the connecting wires (15) include a wire (151) and connected to the two ends of the wire (151) and are adapted to the DC power socket (14). Plug (152); The plugs (152) at both ends of the connection line (15) are respectively connected to the DC power sockets (14) on different incubator units correspondingly. 7. A kind of tissue engineering dynamic culture instrument that adopts dynamic mechanical stimulation according to claim 6, is characterized in that, described attracting block (4) is button magnet, and described attracting block (4) and described driving motor (2) The distance between the rotation axes of the power output ends is adjustable. 8. A kind of tissue engineering dynamic culture instrument adopting dynamic mechanical stimulation according to claim 7, characterized in that, the rotating support (3) is made of ferromagnetic material, and the attracting block (4) is adsorbed on The rotary support (3) is fixed on the rotary support (3), and a groove (31) is provided on the rotary support (3), and the attraction block (4) is placed in the groove (31) and can be placed on the The groove (31) moves toward or away from the rotation axis of the power output end of the drive motor (2). 9. A kind of tissue engineering dynamic culture instrument adopting dynamic mechanical stimulation according to claim 6, characterized in that, a support seat (8) is installed inside the box body (1), and the drive motor (2) is fixed on the support seat (8). 10. A kind of tissue engineering dynamic culturing apparatus adopting dynamic mechanical stimulation according to claim 6, characterized in that, it also includes a power adapter (16) whose output voltage is 12v direct current, and one end of the power adapter (16) is connected to 220v AC power supply, and the other end is connected with the described DC power socket (14) in any one of the incubator units. 11. A kind of tissue engineering dynamic culture instrument adopting dynamic mechanical stimulation according to claim 6, characterized in that, the box body (1) is made of a material resistant to high temperature and high pressure, and the box body (1) includes An upper cover (101) and a base (102), the upper cover (101) is hinged on the base (102). 12. A kind of dynamic culture instrument for tissue engineering that adopts dynamic mechanical stimulation according to claim 6, characterized in that, the upper part of the box body (1) is provided with an opening suitable for the outer diameter of the culture bottle (5). An annular retaining ring (13) protruding from the upper surface of the box body (1), the center of the retaining ring (13) is located on the rotation axis of the power output end of the driving motor (2).