CN206089661U - Biological nanometer paramagnetic particle method extracts serum virus RNA kit - Google Patents
Biological nanometer paramagnetic particle method extracts serum virus RNA kit Download PDFInfo
- Publication number
- CN206089661U CN206089661U CN201621121683.7U CN201621121683U CN206089661U CN 206089661 U CN206089661 U CN 206089661U CN 201621121683 U CN201621121683 U CN 201621121683U CN 206089661 U CN206089661 U CN 206089661U
- Authority
- CN
- China
- Prior art keywords
- reagent
- reagent bottle
- plate
- lead screw
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a biological nanometer paramagnetic particle method extracts serum virus RNA kit, includes a box body and box cover, separate for the reagent chamber in the box body and hold the chamber, the reagent intracavity is equipped with tool holder and reagent storage box, place the first reagent bottle that holds lyophilized biological nanometer magnetic bead reagent in the reagent storage box respectively, hold second reagent bottle, the third reagent bottle that holds serum schizolysis buffer solution, the fourth reagent bottle that holds RNA nucleic acid extraction reinforcing reagent, the 5th reagent bottle that holds washing buffer solution I that freeze dry reagent and dilute the buffer solution, hold washing buffer solution II's the 6th reagent bottle and hold dnaseRnase the 7th reagent bottle of free elution buffer solution. This kit structure is simple, and it is more convenient to use, and can be more convenient take, put the reagent bottle effectively improves serum virus RNA's extraction efficiency, provides the adapt circumstance for depositing of reagent simultaneously, effectively portable or deposit reagent.
Description
Technical Field
The utility model belongs to the technical field of it is biological, in particular to serum virus RNA kit is drawed to biological nanometer magnetic bead method.
Background
Magnetic separation technology based on micro-nano magnetic beads is increasingly applied in the fields of life science research, clinical detection and the like since the birth of the 80 th century. The magnetic bead separation technology is simple to operate, strong in adaptability, capable of being manually operated and well suitable for automatic operation of instruments. The biological nanometer magnetic bead is a magnetic nanometer particle synthesized by microorganisms, is a natural biological magnetic nanometer material, and has incomparable advantages compared with artificial synthetic magnetic beads. By utilizing a genetic engineering technology or combining a chemical modification technology, special biological functional molecules can be directionally displayed on the surface of the biological nano magnetic bead, or other substances such as silylated substances are coated, so that the biological nano magnetic bead can be used for purifying and extracting common nucleic acid and specific nucleic acid molecules.
The magnetic bead method for extracting nucleic acid samples has important application value in the fields of life science and medical inspection, but the extraction of RNA components of samples from different sources is more complicated than the extraction of DNA, because the stability of RNA is not as good as that of DNA, a special treatment method is needed, and mature, standard and convenient-to-operate kits are still to be developed.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a biological nanometer magnetic bead method draws serum virus RNA kit, this kit easy operation carries out RNA to the sample of specific source specially and draws, can supply with fields such as scientific research or inspection and quarantine of different demands and use.
The utility model discloses specific technical scheme as follows:
the utility model provides a serum virus RNA kit is drawed to biological nanometer magnetic bead method, including the box body and with box body one side articulated lid, separate for reagent chamber and hold the chamber through the diaphragm in the box body, hold the intracavity be equipped with box body sliding connection's the box of taking out, it is equipped with magnetic means to take out the box, reagent intracavity top bilateral symmetry is equipped with the fixed plate, be equipped with tool holder and reagent storage box in the reagent chamber, the link plate is connected at tool holder both sides top, the link plate overlap joint is in the fixed plate edge, reagent storage box is located the tool holder bottom, place the first reagent bottle that is used for holding freeze-dried biological nanometer magnetic bead reagent in the reagent storage box respectively, be used for holding the second reagent bottle of freeze-dried reagent dilution buffer, be used for holding the third reagent bottle of serum schizolysis buffer, be used for holding the fourth reagent bottle of RNA nucleic acid extraction reinforcing reagent, A fifth reagent bottle for containing washing buffer solution I, a sixth reagent bottle for containing washing buffer solution II and a seventh reagent bottle for containing DNase/RNase-Free elution buffer solution.
Furthermore, a lifting mechanism for lifting the reagent storage box is arranged in the reagent cavity, the lifting mechanism comprises lifting components symmetrically arranged at two sides in the reagent cavity, a bottom plate transversely arranged at the bottom of the reagent cavity and a supporting plate transversely arranged between the two lifting components, and the reagent storage box is placed on the supporting plate; wherein,
the lifting assembly comprises a lead screw, an optical axis, a chuck and a motor, the top end of the lead screw is pivotally connected with the fixed plate through a bearing, the bottom end of the lead screw sequentially penetrates through the chuck and the bottom plate to be connected with an output shaft of the motor, the chuck is in threaded connection with the lead screw, the top end of the optical axis is fixed on the fixed plate, and the bottom end of the optical axis penetrates through the chuck and is fixed on the bottom plate;
two ends of the supporting plate are respectively fixed on the clamping heads of the two lifting components.
Furthermore, a first mounting hole used for being connected with the lead screw in a penetrating mode and a second mounting hole used for being connected with the optical axis in a penetrating mode are longitudinally formed in the clamping head, threads matched with the lead screw are arranged in the first mounting hole, and the optical axis penetrates through the second mounting hole and is used for limiting the clamping head to move axially along the lead screw.
Furthermore, one side of the clamping head, which is close to the supporting plate, is transversely provided with a mounting groove for clamping the supporting plate.
Preferably, the end part of the supporting plate is clamped in the mounting groove, and the side wall of the top of the mounting groove is connected with a bolt for fixing the supporting plate in a penetrating manner.
Preferably, the containing cavity is located at the bottom of the bottom plate, a plurality of ice bags are placed at the bottom of the bottom plate, and through holes are uniformly formed in the bottom plate.
The utility model has the advantages as follows: the utility model provides a reagent storage box simple structure, it is more convenient to use, can be more convenient take, put reagent bottle, effectively improve serum virus RNA's extraction efficiency, for depositing of reagent provides suitable environment simultaneously, effective portable or deposit reagent, the practicality is strong.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional structure of a kit for extracting serum viral RNA according to example 1 by using a biomagnetic bead method;
FIG. 2 is a sectional view of the kit for extracting serum viral RNA according to the biomagnetic bead method described in example 1;
FIG. 3 is a sectional view of the kit for extracting serum viral RNA according to example 2 by biomagnetic beads;
fig. 4 is a schematic view of a three-dimensional structure of a chuck of the kit for extracting serum virus RNA by using the biomagnetic bead method according to embodiment 2.
Wherein: 1. a box body; 2. a box cover; 3. a reagent chamber; 4. an accommodating chamber; 5. drawing the box; 6. a fixing plate; 7. a tool box; 8. a reagent storage cassette; 9. hanging the plate; 10. a first reagent bottle; 11. a second reagent bottle; 12. a third reagent bottle; 13. a fourth reagent bottle; 14. a fifth reagent bottle; 15. a sixth reagent bottle; 16. a seventh reagent bottle; 17. a base plate; 18. a support plate; 19. a lead screw; 20. an optical axis; 21. clamping a head; 22. a motor; 23. a first mounting hole; 24. a second mounting hole; 25. mounting grooves; 26. a bolt; 27. an ice bag; 28. a magnetic device.
Detailed Description
The present invention will be described in further detail with reference to the following examples and drawings.
Example 1
As shown in fig. 1 or 2, embodiment 1 of the utility model provides a biological nanometer magnetic bead method draws serum virus RNA kit, including box body 1 and with 1 one side articulated lid 2 of box body, separate for reagent chamber 3 and hold chamber 4 through the diaphragm in the box body 1, hold be equipped with in the chamber 4 with 1 sliding connection's of box body take out box 5, take out and be equipped with magnetic means 28 in the box 5, magnetic means 28 can be for magnet or magnetic substance, and it can be used for adsorbing the magnetic bead, effectively improves serum virus RNA's extraction efficiency.
The reagent chamber 3 interior top bilateral symmetry is equipped with fixed plate 6, be equipped with tool holder 7 and reagent storage box 8 in the reagent chamber 3, link plate 9 is connected at 7 both sides tops of tool holder, the 9 overlap joints of link plate are in 6 edges of fixed plate, reagent storage box 8 is located 7 bottoms of tool holder, 7 overlap joints of tool holder are in on the fixed plate 6, can conveniently take out, take out after the tool holder 7, can conveniently take out the reagent bottle.
The reagent storage box 8 is internally provided with a first reagent bottle 10 for containing a freeze-dried biological nano magnetic bead reagent, a second reagent bottle 11 for containing a freeze-dried reagent dilution buffer, a third reagent bottle 12 for containing a serum lysis buffer, a fourth reagent bottle 13 for containing an RNA nucleic acid extraction enhancing reagent, a fifth reagent bottle 14 for containing a washing buffer I, a sixth reagent bottle 15 for containing a washing buffer II, and a seventh reagent bottle 16 for containing a Dnase/Rnase-Free elution buffer.
Example 2
The embodiment 2 of the utility model provides a further reagent storage box 8 can pass through elevating system up-and-down motion on embodiment 1's basis to conveniently take the reagent bottle.
As shown in fig. 3, an elevating mechanism for elevating the reagent storage box 8 is further arranged in the reagent chamber 3, the elevating mechanism comprises elevating components symmetrically arranged at two sides in the reagent chamber 3, a bottom plate 17 transversely arranged at the bottom of the reagent chamber 3, and a supporting plate 18 transversely arranged between the two elevating components, and the reagent storage box 8 is placed on the supporting plate 18; the backup pad 18 can be held up to the lifting means that the symmetry set up, because reagent storage box 8 is placed in backup pad 18, for this reason, reagent storage box 8 can be lifted or put down through lifting means, the reagent bottle of more conveniently taking.
As shown in fig. 3, it is further limited in this technical solution that the lifting assembly includes a lead screw 19, an optical axis 20, a chuck 21 and a motor 22, the top end of the lead screw 19 is pivotally connected to the fixing plate 6 through a bearing, the bottom end of the lead screw 19 sequentially passes through the chuck 21 and the base plate 17 to be connected to an output shaft of the motor 22, the chuck 21 is in threaded connection with the lead screw 19, the top end of the optical axis 20 is fixed to the fixing plate 6, and the bottom end of the optical axis 20 passes through the chuck 21 to be fixed to the base plate 17.
The lead screw 19 is in threaded connection with the chuck 21, when the lead screw 19 is driven by the motor 22 to rotate, the chuck 21 rotates along with the lead screw 19, but the chuck 21 can only move up and down along the lead screw 19 due to the fact that the optical axis 20 limits the chuck 21 in the circumferential direction, and lifting is achieved.
The two ends of the supporting plate 18 are respectively fixed on the clamping heads 21 of the two lifting assemblies.
As shown in fig. 4, in the present technical solution, a structure of a chuck 21 is further defined, a first mounting hole 23 for passing through the lead screw 19 and a second mounting hole 24 for passing through the optical axis 20 are longitudinally formed in the chuck 21, a thread adapted to the lead screw 19 is disposed in the first mounting hole 23, and the optical axis 20 passes through the second mounting hole 24 and is used for limiting the chuck 21 to move axially along the lead screw 19. Lead screw 19 passes first mounting hole 23 and with first mounting hole 23 threaded connection, and optical axis 20 passes second mounting hole 24, and when lead screw 19 rotated, because optical axis 20 restricted dop 21's circular motion, so dop 21 can only follow optical axis 20 and lead screw 19 up-and-down motion to realized promoting reagent storage box 8 and descending, effectively conveniently take, put the reagent bottle, improved test efficiency.
As shown in fig. 4, further, a mounting groove 25 for clamping the support plate 18 is transversely formed on one side of the chuck 21 close to the support plate 18. The mounting grooves 25 can be used for clamping the two ends of the supporting plate 18, and therefore, when the clamping head 21 moves up and down along the optical axis 20 and the lead screw 19, the clamping head 21 drives the supporting plate 18 to move up and down, so that the reagent storage box 8 on the supporting plate 18 moves up and down, and reagent bottles are conveniently taken out.
As shown in fig. 4, in order to improve the stability of the supporting plate 18, in the present embodiment, it is limited that the end of the supporting plate 18 is clamped in the mounting groove 25, and a bolt 26 for fixing the supporting plate 18 is threaded through a top side wall of the mounting groove 25. The both ends joint of backup pad 18 is in the mounting groove 25 to bolt up fixedly through the bolt 26 at mounting groove 25 top, effectively improve the stability after the backup pad 18 installation.
In order to provide a suitable preservation environment for the reagent, the technical scheme defines that a plurality of ice bags 27 are placed at the bottom of the bottom plate 17 in the accommodating cavity 4, and through holes are uniformly formed in the bottom plate 17. The cold air in the ice bag 27 is emitted into the reagent cavity 3 through the through hole, so that a proper environment is effectively provided for the preservation of the reagent.
The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by the teaching of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as the present invention, fall within the protection scope of the present invention.
Claims (6)
1. The utility model provides a biological nanometer magnetic bead method draws serum virus RNA kit, including box body (1) and with box body (1) one side articulated lid (2), its characterized in that, separate for reagent chamber (3) and hold chamber (4) through the diaphragm in box body (1), hold be equipped with in chamber (4) with box body (1) sliding connection's box (5) of taking out, it is equipped with magnetic means (28) in box (5) to take out, top bilateral symmetry is equipped with fixed plate (6) in reagent chamber (3), be equipped with tool holder (7) and reagent storage box (8) in reagent chamber (3), link plate (9) is connected at tool holder (7) both sides top, link plate (9) overlap joint is in fixed plate (6) edge, reagent storage box (8) are located tool holder (7) bottom, place first reagent bottle (10) that are used for holding freeze-dried biological nanometer magnetic bead reagent in reagent storage box (8) respectively, A second reagent bottle (11) for holding freeze-drying reagent dilution buffer, a third reagent bottle (12) for holding serum lysis buffer, a fourth reagent bottle (13) for holding RNA nucleic acid extraction enhancing reagent, a fifth reagent bottle (14) for holding washing buffer I, a sixth reagent bottle (15) for holding washing buffer II, and a seventh reagent bottle (16) for holding Dnase/Rnase-Free elution buffer.
2. The kit for extracting serum viral RNA according to claim 1, wherein an elevating mechanism for elevating the reagent storage box (8) is further arranged in the reagent chamber (3), the elevating mechanism comprises elevating components symmetrically arranged at two sides in the reagent chamber (3), a bottom plate (17) transversely arranged at the bottom of the reagent chamber (3) and a support plate (18) transversely arranged between the two elevating components, and the reagent storage box (8) is placed on the support plate (18); wherein,
the lifting assembly comprises a lead screw (19), an optical axis (20), a clamping head (21) and a motor (22), the top end of the lead screw (19) is in pivot connection with the fixing plate (6) through a bearing, the bottom end of the lead screw (19) sequentially penetrates through the clamping head (21) and the bottom plate (17) to be connected with an output shaft of the motor (22), the clamping head (21) is in threaded connection with the lead screw (19), the top end of the optical axis (20) is fixed on the fixing plate (6), and the bottom end of the optical axis (20) penetrates through the clamping head (21) to be fixed on the bottom plate (17);
two ends of the supporting plate (18) are respectively fixed on the clamping heads (21) of the two lifting components.
3. The kit for extracting serum virus RNA according to claim 2, wherein a first mounting hole (23) for penetrating the lead screw (19) and a second mounting hole (24) for penetrating the optical axis (20) are longitudinally formed in the chuck (21), a thread adapted to the lead screw (19) is arranged in the first mounting hole (23), and the optical axis (20) penetrates through the second mounting hole (24) and is used for limiting the chuck (21) to move axially along the lead screw (19).
4. The kit for extracting serum virus RNA according to the biological nanometer magnetic bead method of claim 2, wherein one side of the chuck (21) close to the support plate (18) is transversely provided with an installation groove (25) for clamping the support plate (18).
5. The kit for extracting serum virus RNA according to claim 4, wherein the end of the support plate (18) is clamped in the mounting groove (25), and a bolt (26) for fixing the support plate (18) is connected to the side wall of the top of the mounting groove (25) in a penetrating manner.
6. The kit for extracting serum virus RNA according to claim 2, wherein a plurality of ice bags (27) are arranged in the containing cavity (4) and positioned at the bottom of the bottom plate (17), and through holes are uniformly formed in the bottom plate (17).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621121683.7U CN206089661U (en) | 2016-10-13 | 2016-10-13 | Biological nanometer paramagnetic particle method extracts serum virus RNA kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621121683.7U CN206089661U (en) | 2016-10-13 | 2016-10-13 | Biological nanometer paramagnetic particle method extracts serum virus RNA kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206089661U true CN206089661U (en) | 2017-04-12 |
Family
ID=58483841
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621121683.7U Active CN206089661U (en) | 2016-10-13 | 2016-10-13 | Biological nanometer paramagnetic particle method extracts serum virus RNA kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206089661U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165465A (en) * | 2018-03-27 | 2018-06-15 | 成都曌昊科技有限公司 | A kind of acquisition process kit for acquisition stem cell in tooth |
| CN108624477A (en) * | 2018-06-27 | 2018-10-09 | 盛蕾 | A kind of kit of detection bacterium vaginosis |
| CN113640196A (en) * | 2021-02-03 | 2021-11-12 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
-
2016
- 2016-10-13 CN CN201621121683.7U patent/CN206089661U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165465A (en) * | 2018-03-27 | 2018-06-15 | 成都曌昊科技有限公司 | A kind of acquisition process kit for acquisition stem cell in tooth |
| CN108624477A (en) * | 2018-06-27 | 2018-10-09 | 盛蕾 | A kind of kit of detection bacterium vaginosis |
| CN113640196A (en) * | 2021-02-03 | 2021-11-12 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
| CN113640196B (en) * | 2021-02-03 | 2023-12-01 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206089661U (en) | Biological nanometer paramagnetic particle method extracts serum virus RNA kit | |
| US9551643B2 (en) | Flow cytometric systems for sterile separation of magnetically labeled sample components | |
| CN106124282A (en) | A kind of method secreting body outside lamination centrifugal filtration separation and Extraction | |
| WO2009002580A3 (en) | Method for bacterial lysis | |
| TWI672375B (en) | Cell culture carrier module, bioreactor and cell recovery method | |
| CN103013814B (en) | Nucleic acid purification device | |
| CN206089659U (en) | Biological nanometer paramagnetic particle method draws viral DNA kit | |
| CN206089658U (en) | Biological nanometer paramagnetic particle method extracts serum virus DNA kit | |
| CN108034565A (en) | Nucleic acid sequencing pre-treatment instrument | |
| US20140242614A1 (en) | Magnetic aggregating and washing device for in vitro assays and methods of use thereof | |
| CN206089660U (en) | Biological nanometer paramagnetic particle method draws viral RNA kit | |
| CN201255708Y (en) | Magnetic separation device for high pass bioanalysis separation | |
| ES2740838T3 (en) | Sample collection and processing device | |
| CN206375924U (en) | Nucleic acid purification magnetic frame | |
| CN208121116U (en) | A kind of device of paramagnetic particle method rapidly extracting nucleic acid | |
| CN204325346U (en) | A kind of instrument for extracting nucleic acid volley of rifle fire device | |
| CN205574499U (en) | Coupling ni -NTA's biological nanometer magnetic bead kit | |
| CN206683922U (en) | A kind of tubular type magnetic bead separating device | |
| CN208964947U (en) | A kind of full-automatic nucleic acid extraction purifying instrument | |
| CN205470296U (en) | Express show streptavidin's biological nanometer magnetic bead kit | |
| CN206616238U (en) | Magnetic bionanoparticles kit | |
| CN207689505U (en) | A kind of amination modified biological nanometer magnetic bead kit | |
| CN214654835U (en) | Kit for extracting serum virus nucleic acid by biological nano magnetic bead method | |
| CN206736280U (en) | A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen | |
| CN214525422U (en) | Safe transport case of biological reagent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180423 Address after: 100096 room 916, King Yan Long building, Hui lung Guan Town, Changping District, Beijing. Patentee after: Beijing nine Lianhua Culture Communication Co., Ltd. Address before: 100098 Beijing city Haidian District Dazhongsi No. 13 Building No. 1 hospital basement A02-28 Patentee before: The state of Beijing Rongzhi Biological Technology Co. Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210324 Address after: 102200 1101, building 3, No.1 a, Chaoqian Road, science and Technology Park, Changping District, Beijing Patentee after: Beijing Jinyuan Liheng Biotechnology Co.,Ltd. Address before: 100096 room 916, King Yan Long building, Hui lung Guan Town, Changping District, Beijing. Patentee before: Beijing nine Lianhua Culture Communication Co.,Ltd. |
|
| TR01 | Transfer of patent right |