CN206033776U - Adherence / suspension type cell culture unit, device and system - Google Patents
Adherence / suspension type cell culture unit, device and system Download PDFInfo
- Publication number
- CN206033776U CN206033776U CN201620828857.7U CN201620828857U CN206033776U CN 206033776 U CN206033776 U CN 206033776U CN 201620828857 U CN201620828857 U CN 201620828857U CN 206033776 U CN206033776 U CN 206033776U
- Authority
- CN
- China
- Prior art keywords
- cell
- cell culture
- culture
- unit
- adherent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 97
- 239000000725 suspension Substances 0.000 title claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 74
- 230000010261 cell growth Effects 0.000 claims abstract description 40
- 230000001464 adherent effect Effects 0.000 claims description 39
- 210000004349 growth plate Anatomy 0.000 claims description 32
- 238000007667 floating Methods 0.000 claims description 27
- 238000000926 separation method Methods 0.000 claims description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000012531 culture fluid Substances 0.000 claims description 6
- 239000012466 permeate Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 239000011550 stock solution Substances 0.000 claims description 5
- 238000012549 training Methods 0.000 claims description 5
- 230000001413 cellular effect Effects 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 3
- 102000008186 Collagen Human genes 0.000 claims description 3
- 108010035532 Collagen Proteins 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 3
- 229920001503 Glucan Polymers 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 229920005830 Polyurethane Foam Polymers 0.000 claims description 3
- 229940072056 alginate Drugs 0.000 claims description 3
- 235000010443 alginic acid Nutrition 0.000 claims description 3
- 229920000615 alginic acid Polymers 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 229920000159 gelatin Polymers 0.000 claims description 3
- 239000008273 gelatin Substances 0.000 claims description 3
- 235000019322 gelatine Nutrition 0.000 claims description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims description 3
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- QNILTEGFHQSKFF-UHFFFAOYSA-N n-propan-2-ylprop-2-enamide Chemical compound CC(C)NC(=O)C=C QNILTEGFHQSKFF-UHFFFAOYSA-N 0.000 claims description 3
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 3
- 229920000728 polyester Polymers 0.000 claims description 3
- -1 polyethylene Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000004626 polylactic acid Substances 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 239000011496 polyurethane foam Substances 0.000 claims description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims 1
- 241001597008 Nomeidae Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 12
- 230000008569 process Effects 0.000 abstract description 4
- 238000004114 suspension culture Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 53
- 238000005516 engineering process Methods 0.000 description 14
- 239000000047 product Substances 0.000 description 14
- 238000011081 inoculation Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000011534 incubation Methods 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 229960005486 vaccine Drugs 0.000 description 6
- 241000700605 Viruses Species 0.000 description 5
- 238000005096 rolling process Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 241000699802 Cricetulus griseus Species 0.000 description 2
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 2
- 241001282153 Scopelogadus mizolepis Species 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000019522 cellular metabolic process Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 210000002249 digestive system Anatomy 0.000 description 2
- 235000012489 doughnuts Nutrition 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 229920000193 polymethacrylate Polymers 0.000 description 2
- 229960003127 rabies vaccine Drugs 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 101100230376 Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) celI gene Proteins 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241000282552 Chlorocebus aethiops Species 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000282894 Sus scrofa domesticus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- SPSXSWRZQFPVTJ-ZQQKUFEYSA-N hepatitis b vaccine Chemical compound C([C@H](NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCSC)C(=O)N[C@@H](CC1N=CN=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)OC(=O)CNC(=O)CNC(=O)[C@H](C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@@H](N)CCCNC(N)=N)C1=CC=CC=C1 SPSXSWRZQFPVTJ-ZQQKUFEYSA-N 0.000 description 1
- 229940124724 hepatitis-A vaccine Drugs 0.000 description 1
- 229940124736 hepatitis-B vaccine Drugs 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 229940032021 tetramune Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000003501 vero cell Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses an adherence / suspension type cell culture unit, device and system relates to cell culture device technical field. It includes the casing to cultivate the unit, be equipped with a plurality of cell growth board in the casing, adjacent cell growth board in turn with the left side wall or the right side wall connection of casing, just the leading flank of cell growth board is fixed with the preceding curb plate of casing, and the trailing flank is fixed with the posterior lateral plate of casing, keeps the clearance between the adjacent cell growth board, constitutes circuitous one -way runner between the inner wall of cell growth board and casing, the position of rest of one -way runner is equipped with the inlet, the termination position of one -way runner is equipped with the liquid outlet. The unit has the characteristics that simple structure, convenient to use, cultivation process monitored, can satisfy extensive zooblast adherence and suspension culture easily.
Description
Technical field
This utility model is related to cell culture system technical field, more particularly to a kind of adherent/floating type cell culture list
Unit, apparatus and system.
Background technology
The external pilot scale culture of cell is a kind of high efficiency method for producing biological product.In the 60-70 ages in 20th century, it is used for
The microcarrier cultural system and doughnut cell culture technology of large-scale culture cell is just founded.Nearly recent decades, due to
The mankind surge to the demand of the biological product such as growth hormone, interferon, monoclonal antibody, vaccine and interleukin, using biography
The Measurement for Biochemistry of system obtains the amount of biological product from animal tissue and far can not meet demand.With cell culture
Principle is become better and approaching perfection day by day with method, and cell large-scale culture technology gradually tends to ripe.
So-called cell large-scale culture technology（large-scale culture technology）Refer in artificial condition
Under（Setting ph, temperature, dissolved oxygen etc.）, in bioreactor（bioreactor）Middle-high density mass propgation is used for producing life
The cell culture technology of Tetramune.At present can large-scale culture cell on the one hand have Ren sus domestica, monkey kidney, Ren Mus etc. it is various primary
Cell and human diploid cell；On the other hand there is cho（Chinese Hamster Ovary）Cell, BHK-21 (hamster kidney cell), Vero are thin
The continuous cell lines such as born of the same parents' (African green monkey kidney passage cell, it is the fibroblast of anchorage dependence).Existing scale cell
Culture is successfully used in producing rabies vaccine, foot-and-mouth disease vaccine, Hepatitis A Vaccine, hepatitis B vaccine, erythrocyte life
Various biological product such as Cheng Su, monoclonal antibody.First generation cell culture technology is carried out by rolling bottle (rollerbottle)
A kind of technology of attached cell culture, referred to as spinner culture technology, the technology have high labor intensive, space service efficiency low
（Unit volume provide cell growth surface area is little, cell density is low）, product quality heterogeneity, the difficult monitoring of incubation
The shortcomings of precise control is unable to environmental condition.With the continuous development of biotechnology, in the urgent need to production is stable and achievable
The mass cell culture process of monitor in real time, can particularly cultivate the work of the mammalian cell of expression specificity albumen
Skill, to obtain a large amount of useful cell expression products.The culture process of traditional glass square vase or rolling bottle, can not meet thin
The demand of born of the same parents' quantity and its efficient secretion product.
Since the seventies, Microcarrier Cell Culture Techniques and bioreactor（Bioreactor）Obtain sending out faster
Exhibition, the species of bioreactor are more and more, and scale is increasing, and more typical biological reactor for cell culture is free air stripping liter
Reactor, doughnut pipe reactor, still stirred reactor and basket bioreactor etc..Since the eighties, gradually open
Begin to replace mouse peritoneal injection hybridoma acquisition ascites to obtain using micro- load biological reactor process culture hybridoma
The method of monoclonal antibody.Zooblast is a kind of eukaryotic cell of acellular wall, and poor growth is very quick to culture environment
Sense.Zooblast mass propgation is carried out using traditional biochemical technology, nutrition necessary to be only capable of meeting incubation will
Ask outer, also lack and set up rational Controlling model, realize pH and dissolved oxygen（DO）Deng the accuracy controlling of condition of culture.Cell biological is anti-
Answer device be precisely controlled the input and output of the gas and liquid added in reactor by microcomputer, maintain cell culture fluid
Optimal pH value and dissolved oxygen level, the abundant and necessary growth for meeting zooblast is to pH value, dissolved oxygen and other nutrients
Demand.At present microcarrier bioreactor has been applied to polytype cell culture, such as 293T cells, sarcoplast,
Vero cells, Chinese hamster ovary celI, but as microcarrier is there are certain requirements to the growth characteristics of cell, still have various kinds of cell adopt
Microcarrier is cultivated, and the cell of existing available microcarrier culture, on culture scale is amplified also there is technology bottle
Neck.With tremendous development of the cell culture technology in terms of scale and reliability, the biological product obtained by cell culture are demonstrate,proved
Bright is that safely and effectively, cell source biological product have obtained the accreditation of society.No matter for animals or the bio-pharmaceutical of people and life
Thing vaccine, all starts to select cell culture technology to be produced, especially relatively large to those molecular weight, structure is more complicated or has
Complicated glycosylation（glycosylated）For the protein of modification, cell culture becomes the mode of production of first-selection.
The beginning of the sixties, Britain AVRI institutes in attached cell system BHK21 cultivate foot and mouth disease viruses after success, from most
First 200ml and 800ml glass containers start, and are amplified to the culture scale of 30L and 100L stainless cylinder of steel soon.1967 with
Afterwards, Wellcome（It is now Cooper animal healths）Group is located at Europe, Africa and 8 national productions of South America under passing through
This technology is applied to foot-and-mouth disease vaccine and veterinary rabies vaccine industrialized production by manufacturer, and the cell tank for having grasped 5000L is big
Scale evaluation technique.
At present, domestic many enterprises are still produced using rolling bottle technology, and having yet with rolling bottle technology can not be big
Scale prepares that mass discrepancy between product, production batch is big, the shortcoming that product cannot be monitored, and country prohibites new rolling bottle production
The Shen of line batch.
It is although microcarrier bioreactor, abroad using relatively broad, microcarrier culture process complex operation, right
Bioreactor has high demands, and domestic equipment is completely dependent on import.At present domestic enterprise to the technique of microcarrier not
Can grasp completely, only the culture scale of 300L can be accomplished by minority enterprise, also only have minority enterprise in the world and grasped 1000L
Culture technique above, it is impossible to the culture scale more than 5000L, seriously constrains the research and development and life of vaccine and bioengineered pharmaceuticals
Produce.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of adherent/floating type cell culture unit, device, is
There is simple structure, easy to use, incubation easily to monitor, can meet extensive zooblast for system and method, the unit
Culture feature.
For solving above-mentioned technical problem, technical solution adopted in the utility model is：A kind of adherent/floating type cell training
Foster unit, it is characterised in that：Including housing, several cell growth plates in the housing, are provided with, adjacent cell growth plate is handed over
It is replacing to be connected with left side wall or right side wall of the housing, and the leading flank of the cell growth plate is solid with the front side board of housing
Calmly, trailing flank is fixed with the back side panel of housing, and retention gap between adjacent cell growth plate, cell growth plate are interior with housing
Roundabout unidirectional runner is constituted between wall, and the original position of the unidirectional runner is provided with inlet, the termination of the unidirectional runner
Position is provided with liquid outlet.
Further technical scheme is：The leading flank of the cell growth plate is vertical with the header board of the housing to be connected,
It is parallel to each other between cell growth plate and cell growth plate.
Further technical scheme is：The inlet is provided with feed tube, and the liquid outlet is provided with drain pipe.
Further technical scheme is：The outer surface of the cell growth plate is provided with good one layer of cellular affinity
Or loose porous or surface lubricious material more than two-layer.
Further technical scheme is：The material is cellulose and its derivates, polyester fiber, shitosan and its spreads out
Biology, collagen protein, gelatin, polylactic acid, polyethylene, polystyrene, polymethacrylates, polyurethane foam microcarrier, sea
Alginate, glucosan, glass, ceramics and/or poly-N-isopropyl acrylamide.
The invention also discloses a kind of adherent/floating type cell culture system, it is characterised in that：Including several institute
The drain pipe of the cell culture unit stated, the feed tube of a cell culture unit and another cell culture unit
Connection, makes to constitute cascaded structure between several cell culture units；The feed tube of described cell culture unit and another
The cell culture unit feed tube connection, described cell culture unit drain pipe are gone out with cell culture unit another described
Liquid pipe connects, and makes to constitute parallel-connection structure between several cell culture units.
The invention also discloses a kind of adherent type cell culture system, it is characterised in that：Fill including described culture
Put, also including device for storing liquid and first circulation pump, the liquid outlet of the device for storing liquid is entered by pipeline and the culture apparatuses
Liquid mouth connects, and the liquid outlet of the culture apparatuses is connected with the inlet of the device for storing liquid by pipeline, the first circulation
Pump is arranged on the pipeline, for driving the media flow in the culture apparatuses.
Further technical scheme is：The device for storing liquid employing can control temperature, dissolved oxygen, the fermentation tank of pH parameters.
The invention also discloses a kind of floating type cell culture system, it is characterised in that：Fill including described culture
Put, also including device for storing liquid, first circulation pump, second circulation pump and cell separation apparatus, the liquid outlet of the device for storing liquid leads to
The inlet that pipeline is crossed with the permeate room of described cell separation apparatus is connected, and the inlet of the device for storing liquid passes through pipeline
Be connected with the liquid outlet of the permeate room of described cell separation apparatus, the inlet of described culture apparatuses by pipeline with it is thin
The stock solution room liquid outlet connection of born of the same parents' segregation apparatuss, the liquid outlet of described culture apparatuses pass through the original of pipeline and cell separation apparatus
The inlet connection of liquid room, described first circulation pump and second circulation pump are respectively provided with
On the pipeline of device, for driving the culture fluid and suspension cell flowing in the culture apparatuses.
Using the beneficial effect produced by above-mentioned technical proposal it is：Described adherent type cell culture unit is by slipstream
Technology is combined with bed technology, has simple structure concurrently, technological operation is easy, product quality is stable, incubation
The advantage for easily monitoring, what energy was stable carry out mass cell amplification cultivation.
The device for storing liquid of adherent/floating type cell culture system of the present utility model, can be controlled by fluid infusion adherent/outstanding
The condition of culture of the whole cell cultivation process of floating type cell culture system, has been coordinated with adherent/floating type cell culture system
Into whole cell cultivation process.
Description of the drawings
With reference to the accompanying drawings and detailed description this utility model is described in further detail.
Fig. 1 is the dimensional structure diagram of this utility model embodiment cell culture unit；
Fig. 2 is the positive structure diagram after this utility model embodiment cell culture unit removes front side board；
Fig. 3 is the structural representation of right plate in this utility model embodiment cell culture unit；
Fig. 4 is the theory diagram of the adherent type cell culture system of this utility model embodiment；
Fig. 5 is the theory diagram of this utility model embodiment floating type cell culture system
Wherein：1st, housing 2, cell growth plate 3, runner 4, inlet 5, liquid outlet 6, feed tube 7, the training of drain pipe 8, cell
Foster unit 9, culture apparatuses 10, device for storing liquid 11, first circulation pump 12, second circulation pump 13, cell separation apparatus.
Specific embodiment
With reference to the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out clearly
Chu, it is fully described by, it is clear that described embodiment is only a part of embodiment of the present utility model, rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment for being obtained, belongs to the scope of this utility model protection.
Many details are elaborated in the following description in order to fully understand this utility model, but this practicality is new
Type can also be different from alternate manner described here to implement using other, and those skilled in the art can be without prejudice to this reality
With doing similar popularization in the case of new intension, therefore this utility model is not limited by following public specific embodiment.
As Figure 1-Figure 2, a kind of adherent/floating type cell culture list is disclosed in one embodiment of the present utility model
Unit, including housing 1, is provided with several cell growth plates 2 in the housing.Adjacent cell growth plate 2 alternately with the shell
The left side wall of body or right side wall connection, and the upper plate of the upper side of the cell growth plate 2 and housing fixes, downside and shell
The lower shoe of body is fixed, retention gap between adjacent cell growth plate 2, is constituted between cell growth plate 2 and the inwall of housing 1
Roundabout unidirectional runner 3, in cell cultivation process, flow direction of the culture medium in cell culture unit is parallel to cell
Growth plate.The original position of the unidirectional runner 3 is provided with inlet 4, and the final position of the unidirectional runner 3 is provided with liquid outlet 5,
Certainly the inlet and liquid outlet of the unit is not significantly distinguished, therefore is capable of achieving to be inverted and is used, using liquid outlet as entering
Liquid mouth, inlet can equally realize function described in the utility model as liquid outlet.
Need to consume substantial amounts of nutrition during due to cell growth, can also produce substantial amounts of metabolic waste, therefore
Normal growth that culture fluid just can guarantee that cell is changed in cell cultivation process constantly.Meanwhile, albumen or virus are existed in
In culture fluid, the utilization rate of equipment and installations that can just obtain maximum is timely harvested.Thus, carry out changing liquid using tangential Flow Technique and fit very much
Cell culture process is closed, simple structure is had concurrently, technological operation is easy, product quality is stable, the advantage of the easy monitoring of incubation,
What energy was stable carries out mass cell amplification cultivation.
As shown in Fig. 2 in one embodiment of the present utility model, downside and the shell of the cell growth plate 2
The lower shoe of body 1 vertically connects, and is parallel to each other between cell growth plate 2 and cell growth plate 2.It is pointed out that the institute
That states 2 entirety of cell growth plate can not be vertical with the lower shoe of the housing 1, can equally realize this utility model, simply carefully
The adherent effect of born of the same parents does not have vertical good.In the same manner, can not also be parallel to each other between cell growth plate 2 and cell growth plate 2, as long as
Not reporting to the leadship after accomplishing a task just can be with.
In one embodiment of the present utility model, as shown in figure 3, the inlet 4 is provided with feed tube 6, it is described go out
Liquid mouth 5 is provided with drain pipe 7, for easily realizing the serial or parallel connection between unit and unit, forms culture apparatuses.
Furthermore, it is necessary to explanation, in order that cell is easier being affixed on the cell growth plate, the cell growth
The outer surface of plate 2 be provided with cellular affinity good a layer or two-layer more than loose porous or surface lubricious material.It is preferred that
, the good material of cellular affinity can adopt cellulose and its derivates, polyester fiber（Nylon）, shitosan and its derivative
Thing, collagen protein, gelatin, polylactic acid, polyethylene, polystyrene, polymethacrylates, polyurethane foam microcarrier, sea
The materials such as alginate, glucosan, glass, ceramics or poly-N-isopropyl acrylamide or above-mentioned material combine and make.
In one embodiment of the present utility model, a kind of adherent/floating type cell culture system is disclosed, including some
Individual described cell culture unit 8, feed tube 6 and another described cell culture unit 8 of a cell culture unit 8
Drain pipe 7 connect, make to constitute cascaded structure between several cell culture units 8；The feed liquor of described cell culture unit 8
Pipe 6 is connected with feed tube 6, and drain pipe 7 is connected with drain pipe 7, makes to constitute parallel-connection structure between several cell culture units 8.
It should be noted that specifically needing to be selected according to actual needs using cascaded structure or parallel-connection structure.
In one embodiment of the present utility model, as shown in figure 4, disclosing a kind of adherent type cell culture system, wrap
Include described culture apparatuses 9, device for storing liquid 10 and first circulation pump 11.The liquid outlet of the device for storing liquid 10 passes through pipeline and institute
The inlet 4 for stating culture apparatuses connects, and the liquid outlet 5 of the culture apparatuses 9 passes through the feed liquor of pipeline and the device for storing liquid 10
Mouth connection, the first circulation pump 11 is arranged on the pipeline, for driving the media flow in the culture apparatuses.It is excellent
Choosing, total system using the fermentation tank that can control temperature, dissolved oxygen, pH parameters, or can be installed on constant temperature ring by device for storing liquid 10
In border.
In one embodiment of the present utility model, as shown in figure 5, disclosing a kind of floating type cell culture system, wrap
Described culture apparatuses 9 are included, also including device for storing liquid 10, first circulation pump 11, second circulation pump 12 and cell separation apparatus 13.
The liquid outlet of the device for storing liquid 10 is connected with the inlet of the permeate room of described cell separation apparatus 13 by pipeline, institute
The inlet for stating device for storing liquid 10 is connected with the liquid outlet of the permeate room of described cell separation apparatus 13 by pipeline, described
The inlet of culture apparatuses 9 be connected with the stock solution room liquid outlet of cell separation apparatus 13 by pipeline, described culture apparatuses 9
Liquid outlet be connected with the stock solution room inlet of cell separation apparatus 13 by pipeline, described first circulation pump 11 and second is followed
Ring pump 12 is respectively provided with and is respectively arranged on the pipeline of connection culture apparatuses 9 and device for storing liquid 10, for driving the culture
Culture fluid in device and suspension cell flowing.
The device for storing liquid of adherent/floating type cell culture system of the present utility model, can control adherent type by fluid infusion
The condition of culture of the whole cell cultivation process of cell culture system, is coordinated with adherent/floating type cell culture system and completes whole
Individual cell cultivation process.
A kind of Wall-attachment cell culture method, comprises the steps：
It is adherent：Adherent type cell culture system and cell inoculation bottle are carried out into aseptic connection, cell is connect by circulating pump
Inoculation liquid circulation in kind of bottle is sent in adherent type cell culture system, and concentrated solution to be seeded is by adherent type cell culture system
When all runners in each unit are just full of, the inlet of adherent type cell culture system, liquid outlet are closed, in constant temperature ring
A period of time is stood in border, and cell can be adhered on cell growth plate, reach adherent purpose.When inoculating cell reaches 40-
After 60% is adherent, adherent type cell culture system is placed upside down；Or after cell is completely adherent, new cell is inoculated with again（With
Inoculation operation first is identical）, after inoculation terminates, it is inverted cell culture system and constant temperature stands a period of time, after cell attachment, i.e.,
Culture link can be proceeded to.
Culture：Adherent type cell culture system is connected according to Fig. 4 with device for storing liquid, device for storing liquid can be adopted and can control temperature
Degree, dissolved oxygen, the fermentation tank of pH parameters（Or total system is installed in isoperibol）, it is ensured that in cell cultivation process at environment
In stable state, in incubation, culture medium is injected in the runner of adherent type cell culture system by peristaltic pump, to cell
While nutrition is provided, products of cellular metabolism is taken away.
Digestion：After culture terminates, the culture medium in adherent type cell culture system is quickly drained, add Digestive system, it is quiet
After putting a period of time, Digestive system and cell are discharged by liquid outlet, can not be such as emptied completely and fresh training can be added by inlet
Foster base, collects cell by liquid outlet.
Infection and results virus:For production of vaccine, virus can be added after culture terminates, treats that virus reaches results density
Afterwards, virus liquid is collected by liquid outlet.
Cultural method described in the utility model, by using culture systems, can stablize, efficient cultured cells, fit
Close mass cell culture.
A kind of floating type cell culture processes, comprise the steps：
Cell is inoculated with：Floating type cell culture system is attached with cell inoculation bottle, will be thin by circulating pump circulation
Inoculation liquid in born of the same parents' inoculation bottle is sent in adherent type cell culture system so that inoculation liquid is each by floating type cell culture system
All runners of culture unit are full of；
Culture：Floating type cell culture system is connected with device for storing liquid, cell separation apparatus, in incubation, is led to
Cross circulating pump 11 culture medium with cell suspension is injected in the runner of institute's floating type cell culture system, cell culture
The flowing scouring force of liquid causes cell suspension and does not settle, while the culture fluid injection cell separation of liquid containing bottle is filled by circulating pump 12
Put so that fresh culture medium and old culture medium occur to exchange, while to cells with nutrient, by products of cellular metabolism
Take away, and cell is not taken away.
Claims (9)
1. a kind of adherent/floating type cell culture unit, it is characterised in that：Including housing（1）, in the housing, it is provided with several
Cell growth plate（2）, adjacent cell growth plate（2）Alternately it is connected with the left side wall or right side wall of the housing, and it is described
Cell growth plate（2）The front side board of leading flank and housing fix, the back side panel of trailing flank and housing is fixed, adjacent cell life
Long slab（2）Between retention gap, cell growth plate（2）With housing（1）Inwall between constitute roundabout unidirectional runner（3）, institute
State unidirectional runner（3）Original position be provided with inlet（4）, the unidirectional runner（3）Final position be provided with liquid outlet（5）.
2. adherent/floating type cell culture unit as claimed in claim 1, it is characterised in that：The cell growth plate（2）'s
Leading flank and the housing（1）Header board vertically connect, cell growth plate（2）With cell growth plate（2）Between be parallel to each other.
3. adherent/floating type cell culture unit as claimed in claim 1, it is characterised in that：The inlet（4）It is provided with
Feed tube（6）, the liquid outlet（5）It is provided with drain pipe（7）.
4. adherent/floating type cell culture unit as claimed in claim 1, it is characterised in that：The cell growth plate（2）'s
Outer surface be provided with cellular affinity good a layer or two-layer more than loose porous or surface lubricious material.
5. adherent/floating type cell culture unit as claimed in claim 4, it is characterised in that：The material be cellulose and
Its derivant, polyester fiber, Chitosan-phospholipid complex, collagen protein, gelatin, polylactic acid, polyethylene, polystyrene, poly- first
Base acrylate, polyurethane foam microcarrier, alginate, glucosan, glass, ceramics and/or poly-N-isopropyl acrylamide.
6. a kind of adherent/floating type cell culture system, it is characterised in that：Including any one in several such as claim 1-5
Cell culture unit described in（8）, a cell culture unit（8）Feed tube（6）Train with cell another described
Foster unit（8）Drain pipe（7）Connection, makes several cell culture units（8）Between constitute cascaded structure；Described cell training
Foster unit（8）Feed tube（6）With cell culture unit another described（8）Feed tube（6）Connection, described cell culture list
Unit（8）Drain pipe（7）With cell culture unit another described（8）Drain pipe（7）Connection, makes several cell culture units
（8）Between constitute parallel-connection structure.
7. a kind of adherent type cell culture system, it is characterised in that：Including culture apparatuses as claimed in claim 6（9）, also wrap
Include device for storing liquid（10）With first circulation pump（11）, the device for storing liquid（10）Liquid outlet pass through pipeline and the culture apparatuses
Inlet（4）Connection, the culture apparatuses（9）Liquid outlet（5）By pipeline and the device for storing liquid（10）Inlet
Connection, the first circulation pump（11）It is arranged on the pipeline, for driving the media flow in the culture apparatuses.
8. adherent type cell culture system as claimed in claim 7, it is characterised in that：The device for storing liquid（10）Using can control
Temperature processed, dissolved oxygen, the fermentation tank of pH parameters.
9. a kind of floating type cell culture system, it is characterised in that：Including culture apparatuses as claimed in claim 6（9）, also wrap
Include device for storing liquid（10）, first circulation pump（11）, second circulation pump（12）And cell separation apparatus（13）, the device for storing liquid
（10）Liquid outlet pass through pipeline and described cell separation apparatus（13）Permeate room inlet connection, liquid storage dress
Put（10）Inlet pass through pipeline and described cell separation apparatus（13）Permeate room liquid outlet connection, described training
Foster device（9）Inlet pass through pipeline and cell separation apparatus（13）The connection of stock solution room liquid outlet, described culture apparatuses
（9）Liquid outlet pass through pipeline and cell separation apparatus（13）The connection of stock solution room inlet, described first circulation pump（11）
With second circulation pump（12）It is respectively provided with（9）And device for storing liquid（10）The pipeline on, use
Flow in culture fluid and suspension cell in the culture apparatuses is driven.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620828857.7U CN206033776U (en) | 2016-08-03 | 2016-08-03 | Adherence / suspension type cell culture unit, device and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620828857.7U CN206033776U (en) | 2016-08-03 | 2016-08-03 | Adherence / suspension type cell culture unit, device and system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206033776U true CN206033776U (en) | 2017-03-22 |
Family
ID=58307794
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620828857.7U Withdrawn - After Issue CN206033776U (en) | 2016-08-03 | 2016-08-03 | Adherence / suspension type cell culture unit, device and system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206033776U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047707A (en) * | 2016-08-03 | 2016-10-26 | 广州赛泊泰生物技术有限公司 | Wall adherence/suspension type cell culture unit as well as device, system and method thereof |
| CN114426926A (en) * | 2022-01-16 | 2022-05-03 | 重庆理工大学 | Assembled plate tower type adherent cell reactor and method for culturing hepatocytes |
| US12182482B2 (en) * | 2017-06-16 | 2024-12-31 | Cytiva Sweden Ab | Method for predicting outcome of an modelling of a process in a bioreactor |
-
2016
- 2016-08-03 CN CN201620828857.7U patent/CN206033776U/en not_active Withdrawn - After Issue
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047707A (en) * | 2016-08-03 | 2016-10-26 | 广州赛泊泰生物技术有限公司 | Wall adherence/suspension type cell culture unit as well as device, system and method thereof |
| CN106047707B (en) * | 2016-08-03 | 2018-11-13 | 广州赛泊泰生物技术有限公司 | Adherent/floating type cell culture unit, device, system and method |
| US12182482B2 (en) * | 2017-06-16 | 2024-12-31 | Cytiva Sweden Ab | Method for predicting outcome of an modelling of a process in a bioreactor |
| CN114426926A (en) * | 2022-01-16 | 2022-05-03 | 重庆理工大学 | Assembled plate tower type adherent cell reactor and method for culturing hepatocytes |
| CN114426926B (en) * | 2022-01-16 | 2023-12-01 | 重庆理工大学 | Prefabricated plate tower type adherent cell reactor and its hepatocyte culture method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106047707B (en) | Adherent/floating type cell culture unit, device, system and method | |
| CN100389193C (en) | A method for safe continuous closed cell culture, virus production and inactivation | |
| US5073491A (en) | Immobilization of cells in alginate beads containing cavities for growth of cells in airlift bioreactors | |
| CN206033776U (en) | Adherence / suspension type cell culture unit, device and system | |
| CN102703319B (en) | Anchorage-dependent cell culture device and anchorage-dependent cell culture system | |
| Van der Velden-deGroot | Microcarrier technology, present status and perspective | |
| CN108641960A (en) | A kind of multipurpose bioreactor | |
| WO2021008571A1 (en) | Cell culture method and application thereof based on high-density and continuous inoculation | |
| CN102703374B (en) | Wall-attachment cell culture method | |
| CN103773741A (en) | Method for preparing influenza vaccine by using cage type ventilating and stirring bioreactor | |
| CN102071137A (en) | Device and method for preparing stem cells through continuous perfusion bioreactor/tank (bag) system | |
| CN102807964A (en) | Method for scale-up culture of animal cells | |
| CN104974933B (en) | A kind of extensive continuous several times, which suspend, turns the apparatus and method of expression recombinant protein wink | |
| Malik et al. | Large-scale culture of mammalian cells for various industrial purposes | |
| CN106635768A (en) | Biological microalgae photosynthesis reactor and application method thereof | |
| CN103103237A (en) | Method for cell perfusion culture to produce recombinant protein by microcarrier technology | |
| Gramer et al. | Comparison of cell growth in T-flasks, in micro hollow fiber bioreactors, and in an industrial scale hollow fiber bioreactor system | |
| CN202730113U (en) | Microalgae high-density culture plant | |
| CN102002482B (en) | Method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses | |
| CN202576436U (en) | Adherent cell culture device and adherent cell culture system | |
| CN107988053A (en) | A kind of microalgae culture system for realizing Immobilized culture and waste water advanced purifying | |
| CN107384801A (en) | Closing biological membrane type cultural method for industrialized production microalgae | |
| CN109251847A (en) | Utilize the device and method of sunlight culture photosynthetic microorganism | |
| CN1158388C (en) | Technological process of continuously perfused culture of hybrid tumor and CHO cell to prepare antibody | |
| CN201343538Y (en) | Liquid removing device for culturing microcarrier cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170322 Effective date of abandoning: 20181113 |
|
| AV01 | Patent right actively abandoned |