CN205826676U - High flux full-automatic immunoblotting instrument - Google Patents

Abstract

The utility model discloses a kind of high flux full-automatic immunoblotting instrument, including controlling device, shaking incubating device and add liquid sucking device, described concussion incubating device is arranged on the top of described control device, the described liquid sucking device that adds includes peristaltic pump, conduit and reagent bottle, wherein one end of conduit is fixed on the top of concussion incubating device, for adding/inhale reagent to it.This utility model is a kind of intelligentized full-automatic immunoblotting instrument, automatization and standardized operation is provided for the experiment of all kinds of immunoblotting films, this instrument can be hatched by the concussion of interpolation and discharge, blotting membrane that the self-defining Automatic Program of operator completes plurality of reagents and the step such as washing, thus considerably reduce the time hand-manipulated of operator, at utmost reduce the deviation brought due to manual operations simultaneously, thus significantly improve the reproducibility and reliability of testing result.

Description

High flux full-automatic immunoblotting instrument

Technical field

This utility model relates to Western blot analysis field, particularly relates to a kind of full-automatic immunoblotting of high flux Instrument.

Background technology

Protein immunoblotting (Immunoblotting), also known as Western Blotting, is usually used in identifying certain hatching egg In vain, and albumen can be carried out quantitative and semi-quantitative analysis, at life science field and laboratory medicine (such as tumor-marker quality testing Survey, the pathogenic microorganism examination etc.) field application the widest.This method is shifted from gel by the target protein after will being separated by electrophoresis To a kind of solid support (such as NC film or pvdf membrane), then hybridize with the ligands specific (such as antibody etc.) of known albumen, Testing goal albumen after being developed the color by enzyme system, wherein immune detection part successively include close, Primary antibodies hybridization, clean, Secondary antibody hybridization, clean this five processes, relate to multistep liquid feeding, imbibition, hatch, the operation such as washing, rigorous and complicated.

How protein immunoblotting experiment in early days is had been manually done by operator, and complex steps not only wastes time and energy, detection Inefficiency, it is impossible to scale operates, and the error that manual operation brings easily occurs, affect the reliability of testing result with Repeatability, operator is many with tool latent infection article contacts simultaneously, detects poor stability.

At present, existing several protein trace instrument is for solving the inconvenience that manual operations is brought.Such as Switzerland TECAN company ProfiBlot 48Western blot analyzer, EUROBlotMaster 44 that European Union company of Germany produces and Shenzhen The Tenfly Auto full-automatic immunoblotting assay instrument etc. of Ya Huilong company, can solve Diagnosis of Sghistosomiasis to a certain extent The manual problem of mark, but these products are disadvantageous in that: 1. it is only applicable to detect the immunity of fillet (Strip) size Blotting membrane, is not suitable for detecting the blotting membrane of other size；Can the detection program number of independent operating low, be not suitable for simultaneously many Plant multiple blotting membranes of detection under different condition；3. not having reagent to reclaim function, operator cannot need making according to experiment Valuable reagent carry out reclaiming with recycling；4. high expensive.

Utility model content

Because the drawbacks described above of prior art, this utility model provides a kind of high flux full-automatic immunoblotting instrument, Including controlling device, shaking incubating device and add liquid sucking device, described concussion incubating device is arranged on described control device Top, described in add liquid sucking device and include peristaltic pump, conduit and reagent bottle, wherein one end of conduit is fixed on concussion incubating device Top, for adding/inhale reagent to it.

Described control device includes power supply, control circuit plate, touches display screen and control software, is used for controlling described immunity Every operation of trace instrument, operator can set the program of operation as required, described program include run and hatch time Between, the selection of reagent, the volume of liquid, flow velocity, the frequency of concussion, amplitude, the number of times etc. of repetition.

Described concussion incubating device is for providing concussion so that sample and examination in sample (immunoblotting film) incubation step Agent is fully contacted；This concussion incubating device include pallet and tray rack, described pallet be used for placing sample (immunoblotting film) and Reagent, described tray rack for providing the support of pallet, and this tray rack be controlled the control of device can carry out rocking and/or Rotary motion.

Preferably, described control device controls the motion of described tray rack by motor, and this motor can essence Really control angle and speed that tray rack rocks and/or rotates.

Further, the number of described tray rack is one or more, preferably 2-6.

Preferably, the number of described pallet is more than the number of described tray rack, and has sizes specification for operator Select when needed, not only meet the detection of the immunoblotting film of fillet (strip) size, it is also possible to meet small-sized (mini-) detection of the immunoblotting film of, medium-sized (midi-) and operator's custom size；Further, described pallet also may be used Use lattice form, a pallet is divided into the multiple little lattice that size is identical or different.Preferably, described pallet is transparent material Material is made, and the person of being convenient to operation observes；Further, described pallet can be disposable product.

Further, the outfit of each tray rack is a set of adds liquid sucking device, including peristaltic pump, conduit and reagent bottle, one of them One reagent bottle of peristaltic pump, a conduit and correspondence thereof forms a pipeline, and each tray rack can be equipped with a plurality of (preferably 2- Article 10) pipeline (many conduits), is respectively used to add different reagent in pallet, draws recovered liquid or waste liquid etc.；Described compacted Dynamic pump is for providing the power of suction reagent.

Each tray rack and a set of liquid sucking device one running unit of composition that adds of correspondence thereof in this utility model, thus one Platform full-automatic immunoblotting instrument can have multiple running unit, can simultaneously independent operating under varying experimental conditions, thus Improve detection flux.Further, the different conduits in running unit can use different colors to be distinguish between.

Further, the one end of the many conduits that each tray rack is corresponding is fixed on torr by fixing device by Open Side Down The top of plate rail, for adding/inhale reagent in pallet, the other end of many conduits is inserted into correspondence when adding/inhale reagent Reagent bottle in, the used time can not extract out from reagent bottle.

Preferably, the intermediate body portions of described conduit is divided into flexible pipe, and the personnel that are convenient to operation arbitrarily move the position of conduit.

Preferably, one end that described conduit inserts in reagent bottle is metal straight pipe, can preferably be inserted as the crow flies by conduit In reagent bottle, and it is difficult to displacement；And it is by fixing dress that described conduit is fixed on the preferably fixed solution of the one end above pallet Put the most arranged together for many conduits, to facilitate follow-up location；It is further preferred that the two ends of described conduit are all provided with Which kind of reagent wherein flow be with clear and definite to be equipped with label, facilitates operator to judge.

Preferably, when described conduit is for drawing the reagent in pallet, this conduit is fixed on the one end above tray rack It is additionally provided with the suction nozzle of band point mouth, tray bottom can be goed deep into exhaust the liquid in pallet.

In a kind of preferred implementation of the present utility model, described tray rack is provided with suspension type fixing device, institute State many conduits be fitly arranged in a row by this device and be fixed on tray rack by suspension type, the pipe of described many conduits Mouth can stretch in pallet；Further, the taking and placing of pallet for convenience, this suspension type fixing device has at least two screens；When When fixing device is in the first screens, the mouth of pipe of described conduit stretches in pallet, and this position is the working position of conduit；When fixing dress Putting when being in the second screens, the mouth of pipe of described conduit is away from the upper limb of pallet, and this position is the rest position of conduit, can taking and placing torr Dish.

Preferably, often set adds in liquid sucking device, all at least provided with a specific pipeline, has conduit and/or pallet Interior reagent is back to the function selecting of reagent bottle；Operator can need to select to reclaim this examination by this pipeline according to experiment Agent, thus reduce cost.In a kind of preferred implementation of the present utility model, described recovery pipe is used for reclaiming Primary antibodies Reagent, can significantly reduce the cost of single detection.

Preferably, this utility model also has full-automatic pipeline-cleaning function, calibration of pump function and levelness detecting function.

By optimum program design, after having tested, operator can select full-automatic or Manual-cleaning program every time Complete pipeline-cleaning, to prevent the interference of different reagent between front and back testing.

During adding imbibition by peristaltic pump, can come smart by being actually added into or the operation time of sucking-off, liquid volume Really correction pump moves speed and the volume of liquid, thus improves degree of accuracy, reduces liquid residue during discharge.

Because the out-of-flatness of instrument placed mesa may cause a small amount of inclination of pallet, automatically calibrated by gyroscope, Guarantee that pallet initial state is horizontal, so that it is guaranteed that pallet vibration velocity and angle is accurate, when simultaneously facilitating experiment Waste liquid is discharged smooth and easy.

Preferably, immunoblotting instrument described in the utility model also includes that bracing frame or hook, support frame as described above or hook are used Described conduit, especially flexible pipe in erecting or hanging；In a kind of preferred implementation of the present utility model, described flexible pipe exists The used time is not hung up with hook, takes off in insertion reagent bottle during use from hook.

Preferably, immunoblotting instrument described in the utility model also includes scalable callosity, for conditioning instrumentation level and Highly, the number of described callosity is preferably 4.

As shown in Figure 6, after switching on power, operator is inputted program, program by touch screen to operational process of the present utility model Parameter and ruuning situation shown by display screen, before program is run, horizontal detector can automatically detect tray rack and (i.e. shakes Bed) level, the conduit on tray rack each leads in reagent A, reagent B, reagent C, cleanout fluid and waste liquid, and they are by respective Peristaltic pump provides drawing liquid pressure, sucks complete when reagent adds, and controller sends oscillator signal to shaking table mair motor, and shaking table starts concussion Until running abort.

This utility model has a following Advantageous Effects:

1) this utility model is a kind of intelligentized full-automatic immunoblotting instrument, provides for the experiment of all kinds of immunoblotting films Automatization and standardized operation, this instrument can be completed interpolation and the row of plurality of reagents by the self-defining Automatic Program of operator Go out, the concussion of blotting membrane is hatched and the step such as washing, thus is considerably reduced the time hand-manipulated of operator, the most maximum Degree reduces the deviation brought due to manual operations, thus significantly improves the reproducibility and reliability of testing result；

2) in routine immunization Blot experiment, Primary antibodies is often one of topmost cost, and this utility model has Primary antibodies reclaims function, can significantly reduce the single cost of immune-blotting method by reclaiming antibody reusing；

3) this utility model is furnished with different size of pallet, can meet medium-sized (midi-), small-sized (mini-), film bar respectively (strip) requirement of the blotting membrane of size；

4) this utility model can detect by the multiple different blotting membrane of independent operating simultaneously, and the most each detection can be made by oneself Justice programming is with the requirements of satisfied different detections, for each program, operator can light self-defined various experimental procedures, each Step can be respectively provided with the parameters such as reagent type, pipetting volume, incubation time and number of repetition；

5) suspension type conduit Fixed Design and different screens can the taking and placing of utility tray and the arrangement of Duo Gen conduit, different Color can be easy to distinguish different running units.

Below with reference to accompanying drawing, the technique effect of design of the present utility model, concrete structure and generation is made furtherly Bright, to be fully understood from the purpose of this utility model, feature and effect.

Accompanying drawing explanation

Fig. 1 is the structural representation of a preferred embodiment of the present utility model；

Fig. 2 is the Facad structure schematic diagram of the pallet of embodiment in Fig. 1；

Fig. 3 is the side structure schematic diagram of pallet first screens of embodiment in Fig. 1；

Fig. 4 is the side structure schematic diagram of pallet second screens of embodiment in Fig. 1；

Fig. 5 is the structural representation after the left surface fractionation of embodiment in Fig. 1；

Fig. 6 is annexation and the schematic flow sheet of embodiment in Fig. 1.

Detailed description of the invention

Being illustrated in figure 1 a preferred embodiment of the present utility model, this high flux full-automatic immunoblotting instrument includes control Device 1 processed, concussion incubating device 2 and add liquid sucking device 3, described concussion incubating device 2 is positioned at the top of described control device, institute State control device to include power supply, control circuit plate, touch display screen 11 and control software, for controlling described immunoblotting instrument Every operation, operator can set the program of operation as required, can input or revise the parameter of each step one by one, including institute Need types of agents, volume, concussion frequency, amplitude, incubation time and number of repetition etc..Additionally, the bottom of described control device 1 is also It is provided with four scalable callosity (not shown)s.

Concussion incubating device 2 includes pallet 21 and tray rack 22, and the immunoblotting instrument of the present embodiment has four tray racks 22, they are connected with the motor controlled within device, and after receiving the signal that control circuit plate sends, four pallets can be carried out Rocking and/or rotary motion, this motor is accurately controlled angle and the speed that tray rack rocks and/or rotates.Work as immunity When marking film is hatched in being placed in pallet 21, marking film can be promoted to be fully contacted with reagent by the concussion of tray rack 22, Thus preferably complete reaction.The present embodiment provides the pallet 22 of three kinds of sizes, is respectively suitable for fillet (strip), small-sized (mini-), immunoblotting film medium-sized (midi-)；Described pallet is made of clear material, and for single use product, conveniently makes With and observe.

As in figure 2 it is shown, be both provided with an arch upper arm 23 on each described tray rack 22, fixing in this arch upper arm 23 5 conduits 31 of one row are installed, are to add for the pallet on this tray rack 22 or draw reagent, in the present embodiment institute Stating the effect of conduit (from left to right) and be respectively as follows: the first conduit for adding confining liquid in pallet, the second conduit is for torr Dish add or in pallet inhale primary antibody dilutions, the 3rd conduit in pallet add secondary antibody diluent, the 4th Conduit is for adding cleanout fluid in pallet, and the 5th conduit is for drawing waste liquid in pallet, and wherein second and the 5th leads The head of pipe is provided with suction nozzle 32, can go deep into tray bottom to exhaust the liquid in pallet.

Described arch upper arm 23 has the first screens (Fig. 3) and the second screens (Fig. 4), and the first screens is used for adding imbibition body Time, the second screens is when taking and placing pallet.In addition, it is also possible to use scalable setting of catheter head to realize taking of pallet Put.Described conduit 31 can be passed by the perforate being arranged in arch upper arm 23, as in Fig. 3 and Fig. 4 from the back of the body of arch upper arm 23 After pass, make the other end freely to put.

As it is shown in figure 5, the middle part of described conduit 31 is flexible pipe, can the most arbitrarily bend, flexible pipe is by being arranged at control Multiple peristaltic pumps 33 of device 1 medial surface processed draw liquid, and the other end of flexible pipe is provided with metal straight pipe, every conduit correspondence one Individual reagent bottle 34, inserts metal straight pipe in reagent bottle 34 during use.Front four difference in aforementioned five conduits are corresponding Different reagent bottle, the 5th conduit can be passed through waste liquid cylinder or be passed directly into sewer.Further, the outside of described control device 1 Face is additionally provided with row's metal hanger 35, can be hung on this row's hook, to keep the cleaning of conduit when conduit does not uses And be easy to arrange.The each self-corresponding row's conduit of four tray racks 22 of the present embodiment can by different tubing color in addition Distinguish.Further for being easy to arrange, can be by first to fourth conduit (each pallet of a corresponding tray rack in the present embodiment Frame correspondence one color, totally four kinds of colors) gather suspension and be fixed on a hook, the most corresponding four of such four pallets Then four the 5th conduits (another kind of color) of four tray racks are also gathered suspension and are fixed on the 5th hook by hook On.

The full-automatic immunoblotting instrument of the present embodiment has two kinds of pipeline-cleaning programs, respectively Manual-cleaning program with complete Automatically cleaning procedure.Automatic washing is more thorough, successively uses two kinds of abluents, time slightly to grow (about 9 minutes).Manual-cleaning is again It is simple clear to claim, and only need to use two kinds of abluents, it is only necessary to time-consuming about 40 seconds.The operating instruction of both cleaning ways is all being touched Touching display screen 11 to show, operator only need to get out cleanout fluid according to explanation instruction, is touching display screen 11 by clicking on phase The button answered can start, and instrument will be automatically performed cleaning procedure.

The full-automatic immunoblotting instrument of the present embodiment also has calibration of pump function, and each peristaltic pump can individually be controlled System.Add the accurate operation time of imbibition process by measuring each peristaltic pump, add the volume of sucking liquid, calibrate the shifting of pump Liquid speed and pipetting volume.

The step that above-mentioned high flux full-automatic immunoblotting instrument uses is simple, specifically includes that

1) preparation: prepare enough required reagent, the conduit on hook is inserted in each self-corresponding reagent bottle, and really Protect the position at the bottom of the ferrule of every catheter tip is in bottle；

2) arch upper arm is placed in the second screens, the disposable pallet filling blotting membrane is placed on tray rack, by arch Upper arm is placed in the first screens；

3) select or amendment program；

4) program is run；

5) after program has been run, arch upper arm being placed in the second screens and i.e. can be taken off pallet, immunoblotting completes.

Full-automatic immunoblotting instrument detection flux in the present embodiment is big, can run 4 different programs, disposably simultaneously Can process 1-4 and open different size of blotting membrane, and procedural freedom degree is high, operator can call or revise pre-set programs, it is possible to from Row definition also stores new procedures.

This utility model operation and maintenance is convenient, and security protection is high, and full automation, running without guard, Considerably reduce handling time；The design of pass road, calibration of pump and the time control function optimized, it is ensured that high-precision point Liquid and lower residual, decrease reagent loss, it is ensured that testing result the most reliable and repeated.Additionally, this practicality The novel Primary antibodies that is also equipped with reclaims function, significantly reduces the cost of single detection, unique full-automatic pipeline-cleaning function Can fully avoid potential experiment cross-contamination.

Preferred embodiment of the present utility model described in detail above.Should be appreciated that the ordinary skill people of this area Member just can make many modifications and variations according to design of the present utility model without creative work.Therefore, all technology neck In territory, technical staff passes through logical analysis, reasoning or limited reality on the basis of existing technology according to design of the present utility model Test available technical scheme, all should be in the protection domain being defined in the patent claims.

Claims (10)

1. a high flux full-automatic immunoblotting instrument, it is characterised in that include controlling device, shaking incubating device and add imbibition Device, described concussion incubating device is arranged on the top of described control device, described in add liquid sucking device and include peristaltic pump, conduit And reagent bottle, one end of wherein said conduit is fixed on the top of described concussion incubating device.

2. high flux full-automatic immunoblotting instrument as claimed in claim 1, it is characterised in that described control device include power supply, Control circuit plate, touch display screen and control software.

3. high flux full-automatic immunoblotting instrument as claimed in claim 1, it is characterised in that also include bracing frame or hook, use Described conduit in erecting or hanging.

4. high flux full-automatic immunoblotting instrument as claimed in claim 1, it is characterised in that described concussion incubating device includes Pallet and tray rack.

5. high flux full-automatic immunoblotting instrument as claimed in claim 4, it is characterised in that described control device passes through stepping Motor controls the motion of described tray rack.

6. high flux full-automatic immunoblotting instrument as claimed in claim 4, it is characterised in that described pallet has sizes Specification.

7. high flux full-automatic immunoblotting instrument as claimed in claim 4, it is characterised in that each tray rack is equipped with a set of adding Liquid sucking device, including peristaltic pump, conduit and reagent bottle.

8. high flux full-automatic immunoblotting instrument as claimed in claim 7, it is characterised in that corresponding many of each tray rack is led Pipe, one end of described many conduits is fixed on the top of described tray rack, described conduit by fixing device by Open Side Down Intermediate body portions be divided into flexible pipe, one end that described conduit inserts in reagent bottle is metal straight pipe.

9. high flux full-automatic immunoblotting instrument as claimed in claim 8, it is characterised in that be provided with outstanding on described tray rack Hanging fixing device, described many conduits are fitly arranged in a row by this device and are fixed on tray rack by suspension type.

10. high flux full-automatic immunoblotting instrument as claimed in claim 9, it is characterised in that described suspension type fixing device has There is at least two screens.