CN205528832U - External perfusion system - Google Patents

Abstract

The utility model discloses an extracorporeal perfusion system, which is characterized in that it comprises a liquid storage bottle, a constant temperature water bath, a peristaltic pump, a perfusion chamber, a collection pipe and several conduits, the liquid storage bottle stores nutrient solution, and the first conduit One end is submerged in the nutrient solution, and the other end is connected to the input end of the perfusion chamber through a peristaltic pump. The experimental materials are placed in the perfusion chamber, and the output end of the perfusion chamber is connected to the collection tube through the second conduit. The perfusion chamber is placed in a constant temperature water bath, wherein: The body of the perfusion chamber is cylindrical, with a slender part at the front end and a rubber stopper at the end. The connecting needle of the second catheter is inserted through the rubber stopper, and the slender part is sealed and connected to the first catheter through a silicone tube. The required parts of the utility model can be obtained from the laboratory, which is easy to obtain and low in cost; simple in structure, convenient in disassembly and assembly, ready to be assembled and used at any time; the parts are sealed and connected with each other, and the sealing performance is good.

Description

An extracorporeal perfusion system

technical field

The utility model relates to the field of detection devices for isolated tissue experiments, in particular to an in vitro perfusion system.

Background technique

Sequential inertial stimulation involving different drugs, compounds and different experimental conditions in modern medical and pharmaceutical research is a common mode of modern medical research. At present, sequential inertial experiments are mostly carried out in the form of perfusion or direct dosing. Direct dosing is not currently used because it is easy to cause unevenness of the test substance and instability of precise research. The perfusion experiment requires a special perfusion system, requiring the entire perfusion pipeline to be a sealed pipeline, and the system is expensive.

Utility model content

In order to solve the problems in the background technology, the utility model proposes an extracorporeal perfusion system, which includes a liquid storage bottle, a constant temperature water bath, a peristaltic pump, a perfusion chamber, a collection tube and several conduits, and the nutrient solution is stored in the liquid storage bottle , one end of the first conduit is submerged in the nutrient solution, the other end is connected to the input end of the perfusion chamber through a peristaltic pump, the experimental materials are placed in the perfusion chamber, the output end of the perfusion chamber is connected to the collection tube through the second conduit, and the perfusion chamber is placed in a constant temperature water bath , wherein: the body of the perfusion chamber is cylindrical, with an elongated part at the front end and a rubber stopper at the end, the connecting needle of the second catheter is inserted through the rubber stopper, and the elongated part is sealed and connected to the first catheter through a silicone tube.

Preferably, the perfusion system further includes a water-isolated container, and the perfusion chamber is placed in a constant temperature water bath through the water-isolated container.

In a preferred embodiment, the water-proof container is a beaker.

In a preferred embodiment, the body of the perfusion chamber is a syringe without a pusher head.

In a preferred embodiment, the rubber stopper is a cross-section of a pusher head of a syringe.

Preferably, the perfusion chamber further includes a hollow rubber tube and a 400-mesh nylon mesh, and the hollow rubber stopper presses and fixes the 400-mesh nylon mesh to the inner front end of the body of the perfusion chamber.

In a preferred embodiment, the hollow rubber stopper is a cross-sectional section of a pusher head of a syringe.

In a preferred embodiment, the peristaltic pump is a multi-channel peristaltic pump, and each channel can be connected to a group of first catheters to perform multiple groups of perfusion at the same time.

Beneficial effects of the utility model

The required parts of the utility model can all be obtained from the laboratory, which is easy to obtain and low in cost; simple in structure, convenient to disassemble and assemble, and can be assembled and used at any time; the parts are sealed and connected with each other, and the sealing performance is good. Multi-channel peristaltic pumps can support multiple groups of perfusion at the same time.

Description of drawings

Fig. 1 is the first structure block diagram of the utility model.

Fig. 2 is the second structure diagram of the utility model.

Fig. 3 is a preferred embodiment diagram of the first structure of the present utility model.

Fig. 4 is a preferred embodiment diagram of the second structure of the utility model.

Fig. 5 is a diagram of a preferred embodiment of the first structure of the perfusion chamber of the present invention.

Fig. 6 is a diagram of a preferred embodiment of the second structure of the perfusion chamber of the present invention.

detailed description

The utility model will be further described below in conjunction with embodiment, but protection scope of the utility model is not limited to this:

Embodiment 1: in conjunction with Fig. 1 and Fig. 3, a kind of extracorporeal perfusion system, it comprises liquid storage bottle 1, constant temperature water bath 4, peristaltic pump 3, perfusion small chamber 5, collection pipe 6 and several conduits, described liquid storage bottle 1 The nutrient solution 2 is stored in the nutrient solution 2, one end of the first conduit 7-1 is submerged in the nutrient solution 2, and the other end is connected to the input end of the perfusion chamber 5 through the peristaltic pump 3, and the experimental materials are placed in the perfusion chamber 5, and the output end of the perfusion chamber 5 passes through The second conduit 7 - 2 is connected to the collection pipe 6 , and the perfusion chamber 5 is placed in the constant temperature water bath 4 .

Embodiment 2: With reference to FIG. 2 and FIG. 4 , an extracorporeal perfusion system as described in Embodiment 1, it also includes a water-proof container 8 , and the perfusion chamber 5 is placed in the constant temperature water bath 4 through the water-proof container 8 . In a more optimal embodiment, the water-proof container 8 is a beaker.

Embodiment 3: In combination with FIG. 5, an in vitro perfusion system as described in Embodiment 1 or 2, the perfusion chamber body 5-1 is cylindrical, the front end (nutrient solution input end) has an elongated portion 5-2, and the tail end (The nutrient solution output end) is plugged with a rubber plug 5-3, the second conduit 7-2 is connected to the needle 5-4 and inserted through the rubber plug 5-3, and the elongated part 5-2 is sealed and connected to the first conduit through the silicone tube 5-5 7-1.

Embodiment 4: An extracorporeal perfusion system as described in Embodiment 3, the body 5-1 of the perfusion chamber is a syringe (5 ml) without a pusher head.

Embodiment 5: A kind of extracorporeal perfusion system as described in Embodiment 3 or 4, the rubber stopper 5-3 is the pusher head cross-section of the syringe (5ml).

Embodiment 6: in conjunction with Fig. 6, a kind of extracorporeal perfusion system as described in embodiment 3～5, the perfusion chamber 5 also comprises hollow rubber tube 5-6 and 400 order nylon mesh 5-7, and hollow rubber stopper 5-6 will The 400-mesh nylon mesh 5-7 is extruded and fixed on the inner front end of the perfusion chamber body 5-1.

Embodiment 7: An extracorporeal perfusion system as described in Embodiments 3 to 6, the hollow rubber stopper 5-6 is the cross section of the pusher head of the syringe (5ml).

Embodiment 8: An extracorporeal perfusion system as described in Embodiments 3 to 7, the peristaltic pump 3 is a multi-channel peristaltic pump, and each channel can be connected to a first catheter 7-1 to perform multiple groups of perfusion at the same time.

This system not only has the characteristics of general in vitro culture, but also keeps the culture in a constantly renewed environment, eliminates the feedback effect of cell metabolites and hormones, and enables the culture to receive stimulation treatments of different frequencies, intensities and times. Suitable for studying the release kinetics of hormones.

The nutrient solution 2 can be commonly used nutrient solution, such as MEM, RPMI-1640, M199, etc. The constant temperature water bath 4 can adopt electric heating constant temperature three water tanks, such as model S·HH·W21·600S etc. The peristaltic pump 3 can adopt the multi-channel peristaltic pump of GILSON.

Use process: the experimental materials taken out under sterile conditions (such as gastrointestinal mucosa, etc.) are cleaned with saline solution (such as PBS solution), weighed, placed in the perfusion chamber 5 for incubation and perfusion, and the perfusion speed can be adjusted by itself. During perfusion, the nutrient solution 2 in the liquid storage bottle 1 is powered by the peristaltic pump 3 adjusted to the same speed, and enters the perfusion chamber 5 maintained by the constant temperature water bath 4 at 37°C through the first conduit 7-1. After the tissue state is stabilized, change to the nutrient solution 2 containing stimulating factors and perfuse for a period of time, and then restore the normal nutrient solution 2 (the time for the nutrient solution 2 from the storage bottle 1 to the perfusion chamber 5 can be measured in advance, and the time for the nutrient solution 2 to perfuse from the perfusion The time when the small chamber 5 flows out to the collection tube 6, so as to accurately calculate the experimental process). The perfusate was collected in the collection tube 6 at required time intervals (generally every 5-10 min), and the samples were stored at -20°C for testing.

The specific embodiments described herein are only examples to illustrate the spirit of the present invention. Those skilled in the technical field to which the utility model belongs can make various modifications or supplements to the described specific embodiments or adopt similar methods to replace them, but they will not deviate from the spirit of the utility model or go beyond the appended claims defined range.

Claims (8)

1. an In vitro perfusion system, it is characterised in that it includes liquid containing bottle, thermostatical water bath, peristaltic pump, perfusion cell, collecting pipe And some conduits, described liquid containing bottle stores nutritional solution, one end of the first conduit is submerged in nutritional solution, and the other end is by wriggling Pump connects the input of perfusion cell, places experiment material in perfusion cell, and the outfan of perfusion cell is by the second conduit even Receiving collector, perfusion cell is placed in thermostatical water bath, wherein: described perfusion cell body is tubular, and front end has carefully Long portion, tail end is plugged with rubber stopper, and the second conduit connecting needle is inserted through rubber stopper, and elongate portion is tightly connected first by silica gel tube Conduit.

A kind of In vitro perfusion system the most according to claim 1, it is characterised in that perfusion system also includes water proof container, and perfusion is little Room is placed in thermostatical water bath by water proof container.

A kind of In vitro perfusion system the most according to claim 1, it is characterised in that described water proof container is beaker.

A kind of In vitro perfusion system the most according to claim 1, it is characterised in that described perfusion cell body is to remove to advance head Syringe.

A kind of In vitro perfusion system the most according to claim 1, it is characterised in that described rubber stopper is that the propelling of syringe is transversal Section.

A kind of In vitro perfusion system the most according to claim 1, it is characterised in that perfusion cell also includes hollow rubber pipe and 400 Mesh nylon wire, 400 mesh nylon wire extruding are fixed on the interior forward end of perfusion cell body by hollow rubber plug.

A kind of In vitro perfusion system the most according to claim 6, it is characterised in that described hollow rubber plug is the propelling head of syringe Transversal section.

8. according to a kind of In vitro perfusion system described in any one of claim 1～7, it is characterised in that described peristaltic pump is multiple tracks peristaltic pump.