CN205398622U - Cell sieve - Google Patents

Abstract

The utility model discloses a cell sieve, which comprises an upper end cover (1), a filter device (2) and a lower end cover (3). The cover (3) is sealed and connected, the upper end cover (1) is connected with a liquid inlet (11) for liquid circulation, and the lower end cover (3) is connected with a liquid outlet (33) for liquid circulation. The beneficial effects of the utility model are: the utility model can assemble filter devices with different pore diameters for graded filtration, inject a mixed cell suspension containing a variety of cells from the liquid inlet, and screen the target cells with a diameter larger than the filter hole. The liquid port is injected into the liquid ultrafiltration membrane height to resuspend the target cells on the filter membrane, and the utility model is inverted, and the target cells are released from the liquid inlet, so that various target cells with different diameters can be obtained, and the purity of the target cells obtained is Higher, fewer miscellaneous cells, the utility model is simple in principle, easy to use, simple in structure and easy to manufacture.

Description

a cell sieve

technical field

The utility model relates to a filter device, in particular to a cell sieve.

Background technique

Primary culture is a common method to obtain target cells, which can be divided into tissue block culture method and enzyme digestion method, among which enzyme digestion method is more commonly used. Enzymatic digestion often results in a cell suspension in which a variety of different cells are mixed together. In order to separate target cells from a mixed cell suspension of various cells, a cell filter device is often used. The cell filter device under the prior art conditions is generally cup-shaped, and its bottom is a screen with several filter holes. Only cells with a smaller volume can be obtained after filtering, but the target cells with a larger volume cannot be obtained, and the purity of the cells is not high. In order to obtain a variety of target cells with different volumes and make the obtained target cells more pure, it is necessary to develop a cell sieve to solve the above problems.

Utility model content

The purpose of the utility model is to provide a cell sieve with simple structure and capable of multistage separation.

The utility model is realized through the following technical solutions: a cell sieve, comprising an upper end cover, a filter device and a lower end cover, the upper end cover is sealed and communicated with the filter device, the filter device is sealed and communicated with the lower end cover, and the upper end cover is connected with a liquid for circulation The liquid inlet and the lower end cover are connected with a liquid outlet for liquid circulation.

The upper end cap is a frustum-shaped end cap whose large end communicates with the small end, the liquid inlet is a circular tube, the liquid inlet communicates with the small end of the upper end cap, and the inner diameter of the liquid inlet is the same as that at the small end of the upper end cap .

The filter device is tubular, and a filter membrane is arranged inside the filter device, and several filter holes are arranged on the filter membrane.

The lower end cap is a frustum-shaped end cap whose large end communicates with the small end, the liquid outlet is a round tube, the liquid outlet communicates with the small end of the lower end cap, and the inner diameter of the liquid outlet is the same as that at the small end of the lower end cap .

The through hole in the upper end cover is a frustum-shaped through hole, the diameter of the large end of the through hole in the upper end cover is the same as the inner diameter of the filter device, the through hole in the lower end cover is a frustum-shaped through hole, and the through hole in the lower end cover The diameter of the large end is the same as the inside diameter of the filter unit.

The large end face of the upper end cap is provided with an upper end cap external thread, one end of the filter device is provided with a filter device internal thread connected with the upper end cap external thread, the other end of the filter device is provided with a filter device external thread, and the large end of the lower end cap The end surface is provided with a connecting part, and the connecting part is provided with an internal thread of the lower end cover connected with the external thread of the filter device, and the outer diameter of the connecting part is the same as the outer diameter of the large end surface of the lower end cover.

The liquid inlet is provided with a switch, and the liquid outlet is provided with a switch.

The free end of the switch is provided with a handle.

Compared with the prior art, the utility model has the following advantages and beneficial effects:

(1) The utility model can be used to screen cells with different diameters. By injecting a cell suspension containing a variety of cells from the liquid inlet, and using a filter membrane to screen cells with a diameter larger than the filter hole, the screening efficiency is high and the output Liquid is injected into the liquid port to increase the height of the liquid ultrafiltration membrane and resuspend the target cells on the filter membrane. When the utility model is turned upside down, the screened target cells can be released from the liquid inlet. The utility model has the advantages of simple principle, convenient use, simple structure and easy manufacture;

(2) The utility model can perform graded filtration by assembling a plurality of filter devices with different pore diameter filter membranes, and can obtain a variety of target cells with different diameters, which is convenient to install and disassemble, reliable to seal, and easy to use;

(3) The target cells obtained by the utility model have high purity and less miscellaneous cells, and have wide application prospects and promotion value.

Description of drawings

Fig. 1 is a structural schematic diagram of a cell sieve;

Fig. 2 is the structural representation of upper end cap;

Fig. 3 is the structural representation of filtering device;

Fig. 4 is the structural representation of lower end cap;

Fig. 5 is a structural schematic diagram of a switch;

Among them, 1—upper end cap, 11—liquid inlet, 12—external thread of upper end cap, 2—filter device, 21—internal thread of filter device, 22—external thread of filter device, 23—filter membrane, 3—lower end cap, 31— Connecting part, 32—internal thread of the lower end cover, 33—liquid outlet, 4—switch, 5—handle, 6—through hole.

detailed description

The utility model will be further described in detail below in conjunction with the examples, but the implementation of the utility model is not limited thereto.

Example 1:

As shown in Figure 1, in this embodiment, a cell sieve includes an upper end cover 1, a filter device 2 and a lower end cover 3, the upper end cover 1 is in sealing communication with the filter device 2, the filter device 2 is in sealing communication with the lower end cover 3, and the upper end cover The cover 1 is communicated with a liquid inlet 11 for liquid circulation, and the lower end cover 3 is communicated with a liquid outlet 33 for liquid circulation.

In this embodiment, the filter device 2 is tubular, and a filter membrane 23 is arranged inside the filter device 2 , and several filter holes are arranged on the filter membrane 23 .

In this embodiment, the upper end cap 1 is a frustum-shaped end cap whose large end communicates with the small end, the through hole in the upper end cap 1 is a frustum-shaped through hole, and the large end of the through hole in the upper end cap 1 The diameter is the same as the inner diameter of the filter device 2, the liquid inlet 11 is a circular tube, the liquid inlet 11 communicates with the small end of the upper end cover 1, and the inner diameter of the liquid inlet 11 is the same as the inner diameter of the upper end cover 1 small end.

In the present embodiment, the lower end cover 3 is a frustum-shaped end cover whose large end communicates with the small end, and the through hole in the lower end cover 3 is a frustum-shaped through hole, and the diameter of the large end of the through hole in the lower end cover 3 is related to the filter diameter. The inner diameter of device 2 is the same, and the liquid outlet 33 is a circular tube, and the liquid outlet 33 communicates with the small end of the lower end cover 3, and the inner diameter of the liquid outlet 33 is the same as the inner diameter at the lower end cover 3 small end.

In this embodiment, the large end face of the upper end cap 1 is provided with an upper end cap external thread 12, one end of the filter device 2 is provided with a filter device internal thread 21 connected with the upper end cap external thread 12, and the other end of the filter device 2 A filter device external thread 22 is provided, and the large end surface of the lower end cover 3 is provided with a connection part 31, and the connection part 31 is provided with a lower end cover internal thread 32 connected with the filter device external thread 22, and the outer diameter of the connection part 31 is consistent with the lower end cover 3 The outer diameter of the large end face is the same, and the upper end cover 1, the filter device 2 and the lower end cover 3 are connected by threads.

In this embodiment, the side of the liquid inlet 11 and the side of the liquid outlet 33 are each provided with a circular hole arranged radially, and a switch 4 is housed in the circular hole. The switch 4 is cylindrical and its diameter is larger than that of the inlet. The inner diameter of the liquid port 11 or the liquid outlet 33, the side of the switch 4 is provided with a through hole 6 along the radial direction, the axis of the through hole 6, the axis of the switch 4 and the axis of the liquid inlet 11 intersect at one point, and the axis of the through hole 6 The axis, the axis of the switch 4 and the axis of the liquid outlet 33 intersect at one point.

In this embodiment, the diameter of the through hole 6 is smaller than or equal to the inner diameter of the liquid inlet 11 or the liquid outlet 33 .

In this embodiment, the free end of the switch 4 is provided with a handle 5, which makes turning the switch 4 more convenient.

The above is only a preferred embodiment of the utility model, and does not limit the utility model in any form. Any simple modification or equivalent change made to the above embodiments according to the technical essence of the utility model falls within the scope of the present utility model. Within the protection scope of the present utility model.

Claims (8)

1. a cell sieve, it is characterized in that: include upper end cover (1), defecator (2) and bottom end cover (3), upper end cover (1) seals with defecator (2) and connects, defecator (2) seals with bottom end cover (3) and connects, upper end cover (1) is communicated with the inlet (11) circulated for liquid, bottom end cover (3) is communicated with the liquid outlet (33) circulated for liquid.

2. a kind of cell sieve according to claim 1, it is characterized in that: described upper end cover (1) is for hold greatly the truncated cone-shaped end cap communicated with small end, inlet (11) is pipe, inlet (11) connects with the small end of upper end cover (1), and the internal diameter of inlet (11) is identical with the internal diameter at upper end cover (1) small end place.

3. a kind of cell sieve according to claim 2, it is characterised in that: described defecator (2) is tubulose, is provided with filter membrane (23) in defecator (2), and filter membrane (23) is provided with several filter openings.

4. a kind of cell sieve according to claim 3, it is characterized in that: described bottom end cover (3) is for hold greatly the truncated cone-shaped end cap communicated with small end, liquid outlet (33) is pipe, liquid outlet (33) connects with the small end of bottom end cover (3), and the internal diameter of liquid outlet (33) is identical with the internal diameter at bottom end cover (3) small end place.

5. a kind of cell sieve according to claim 4, it is characterized in that: the described through hole that through hole is truncated cone-shaped in upper end cover (1), the outside diameter of upper end cover (1) inner via hole is identical with the internal diameter of defecator (2), the through hole that through hole is truncated cone-shaped in bottom end cover (3), the outside diameter of bottom end cover (3) inner via hole is identical with the internal diameter of defecator (2).

6. a kind of cell sieve according to claim 5, it is characterized in that: the big end end face of described upper end cover (1) is provided with upper end cover external screw thread (12), one end of defecator (2) is provided with the defecator female thread (21) being connected with upper end cover external screw thread (12), the other end of defecator (2) is provided with defecator external screw thread (22), the large end face of bottom end cover (3) is provided with connecting portion (31), the bottom end cover female thread (32) being connected with defecator external screw thread (22) it is provided with in connecting portion (31), the external diameter of connecting portion (31) is identical with the large end face external diameter of bottom end cover (3).

7. a kind of cell sieve according to claim 6, it is characterised in that: described inlet (11) is provided with switch, and liquid outlet (33) is provided with switch.

8. a kind of cell sieve according to claim 7, it is characterised in that: the free end of described switch is provided with handle (5).