CN204439546U - A kind of Portable fluorescence and chemiluminescence imaging pick-up unit - Google Patents

Abstract

The utility model relates to a portable fluorescence and chemiluminescence imaging detection device. A portable fluorescence and chemiluminescence imaging detection device, which includes a cabinet, a sample workbench, an image acquisition system and a power control system, the interior of the cabinet is divided into a darkroom and a camera room; the image acquisition system includes an excitation light source, a mirror , cooling CCD lens and camera; the sample workbench is placed at the bottom of the dark room, the reflector is placed directly above the center of the sample workbench, the excitation light source is placed above the outside of the sample workbench, and the refrigeration The CCD lens is fixed on the frame and faces the reflective surface of the reflector horizontally, the cooled CCD lens is connected to the camera, and the power control system supplies power to the excitation light source and the camera respectively. The device can collect and image the fluorescence or chemiluminescence signal emitted from the sample. The device is compact in structure, portable and easy to operate, and can meet the needs of simultaneous fluorescence and chemiluminescence imaging detection.

Description

A Portable Fluorescence and Chemiluminescence Imaging Detection Device

technical field

The utility model relates to the field of biological and medical analysis and detection, in particular to a portable fluorescence and chemiluminescence imaging detection device.

Background technique

In the mid-1970s, Arakawe et al. reported that luminescent signals could be used for enzyme immunoassay, that is, the use of luminescent chemical reactions to analyze ultra-trace substances, and the concept of chemiluminescence immunoassay was first proposed. Chemiluminescent immunoassay is a new type of labeled immunoassay technology that combines chemiluminescent or bioluminescent systems with immune reactions to detect trace antigens or antibodies. Its detection principle is similar to that of radioimmunoassay and enzyme immunoassay, except that luminescent substances are used instead of radionuclides or enzymes as markers, and are directly measured with the help of their own luminous intensity.

The principle of the chemiluminescent system is that the enzyme in the immune reaction acts on the luminescent substrate. Under the action of the enzyme, the luminescent substrate undergoes a chemical reaction and releases a large amount of energy to produce an excited state intermediate. This excited state intermediate, when it returns to the stable ground state, simultaneously emits a photon. The light quantum yield can be measured by using the luminescence signal measuring instrument, and the light quantum yield is proportional to the amount of the substance to be measured in the sample. From this, a standard curve can be established and the content of the substance to be tested in the sample can be calculated. Chemiluminescence immunoassay not only has the high sensitivity of radioimmunoassay, but also has the characteristics of simple and fast operation of enzyme-linked immunoassay, easy to standardize operation, and does not use harmful reagents in the test, and the reagents have a long retention period, so it is widely used in biology, Medical research and clinical laboratory diagnosis have become one of the most promising methods in non-radioimmunoassay.

The key to chemiluminescent immunoassay is the detection of luminescent signals. In the early days, it mainly relied on traditional color development or film exposure, which is not easy to analyze because of its low sensitivity or cumbersome operation. Now, the high-cooling CCD digital imaging technology has replaced the traditional color development or film exposure method, and its only disadvantage is that it needs a chemiluminescence imaging instrument. Currently, the chemiluminescent imaging instruments sold in the market are large in size, require a large storage space, and are not easy to move, which limits their application prospects. Therefore, it is necessary to improve it and design and produce a class of portable detection instruments with compact structure and easy operation.

Utility model content

The purpose of this utility model is to provide a portable fluorescence and chemiluminescence imaging detection device, the user can obtain the chemiluminescence or fluorescence signal from the sample to be tested with simpler operations, and store it as a digital image, which is convenient for detection and analysis.

The utility model adopts the following technical solutions:

A portable fluorescence and chemiluminescence imaging detection device, which includes a cabinet, a sample workbench, an image acquisition system and a power control system, the inside of the cabinet is divided into a darkroom and a camera room; the image acquisition system includes an excitation light source, a mirror , cooling CCD lens and camera; the sample workbench is placed at the bottom of the dark room, the reflector is placed directly above the center of the sample workbench, the excitation light source is placed above the outside of the sample workbench, and the refrigeration The CCD lens is fixed on the frame and faces the reflective surface of the mirror horizontally, the cooling CCD lens is connected to the camera, and the power control system supplies power to the excitation light source and the camera respectively.

Preferably, the sample workbench is a movable support structure;

Preferably, the imaging instrument is in the shape of a cuboid as a whole. The lengths of the three adjacent sides of the cuboid are respectively 12-18 cm, 12-18 cm and 35-45 cm, preferably 15 cm, 15 cm and 40 cm.

The utility model can collect and image the fluorescence or chemiluminescence signal emitted from the sample to be tested. The device has a compact structure, is portable and easy to operate, and can meet the needs of simultaneous fluorescence and chemiluminescence imaging detection.

Description of drawings

Fig. 1 is a structural schematic diagram of a preferred embodiment of the utility model.

Detailed ways

Below in conjunction with specific embodiment, further set forth the utility model. These examples are only used to illustrate the utility model and not to limit the scope of the utility model. Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can be applied to the method of the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only.

The utility model relates to a portable fluorescence and chemiluminescence imaging detection device for fluorescence and chemiluminescence detection. The device adopts an integrated industrial design and integrates a darkroom, a sample workbench and an image acquisition system into one. The darkroom 4 is a closed light-tight interval for fluorescence and chemiluminescence imaging; the sample workbench 5 is a movable support structure for placing samples to be tested; the image acquisition system includes an excitation light source 3, Reflective mirror 2, cooling CCD lens 7 and camera 8 are used for luminous signal collection and digital imaging, and are transmitted to the computer through USB interface 11 for storage and analysis.

The power control system includes a control system 1 and a power supply 12 ; a fan 9 and a USB interface 11 are installed on the chassis 10 , and the interior of the chassis 10 is divided into a dark room 4 and a camera room.

The excitation light source 3 is an excitation light source such as ultraviolet, red, green, blue, etc., and can also perform multicolor fluorescence to realize simultaneous detection of two or more target proteins.

The case 10 is provided with a handle for easy movement and transportation.

The cooled CCD lens has a Teflon-coated black EPI tray, which can significantly improve the signal-to-noise ratio.

The utility model adopts an integrated design, integrates a darkroom, a sample workbench and an image acquisition system in one, has a compact structure, effectively saves space, and is a portable instrument;

The image acquisition system of the utility model adopts a refrigerated CCD lens and a camera, and the storage format is a digital image;

The utility model uses a reflector to guide the chemiluminescent or fluorescent signal to the cooling CCD lens and camera, so that the whole device is arranged horizontally and the space is reduced;

The utility model is connected with a computer through a USB interface, and collects, controls and analyzes images through the computer.

The scope of the present invention is not limited by the specific embodiments described, which are only intended as a single example illustrating various aspects of the present invention, and functionally equivalent methods and components are also included within the scope of the present invention. In fact, in addition to the content described herein, those skilled in the art can easily grasp various improvements to the utility model with reference to the above description and accompanying drawings. Such modifications also fall within the scope of the appended claims.

Claims (10)

1. Portable fluorescence and a chemiluminescence imaging pick-up unit, it comprises cabinet, sample worktable, image capturing system and power control system, it is characterized in that described cabinet inside is divided into darkroom and camera chamber; Described image capturing system comprises excitation source, reflective mirror, refrigeration CCD camera lens and camera; Described sample worktable is placed in darkroom inner bottom part, described reflective mirror is placed in directly over described sample worktable central authorities, described excitation source is placed in top outside described sample worktable, described refrigeration CCD camera lens to be fixed on framework and level just to described reflective mirror reflective surface, described refrigeration CCD camera lens connects camera, and described power control system is powered respectively to excitation source and camera.

2. Portable fluorescence according to claim 1 and chemiluminescence imaging pick-up unit, is characterized in that described sample worktable is movable support structure.

3. Portable fluorescence according to claim 1 and chemiluminescence imaging pick-up unit, is characterized in that described Image-forming instrument entirety is in cuboid.

4. Portable fluorescence according to claim 3 and chemiluminescence imaging pick-up unit, is characterized in that the length of described cuboid three adjacent edges is 12 ~ 18cm, 12 ~ 18cm and 35 ~ 45cm respectively.

5. Portable fluorescence according to claim 3 and chemiluminescence imaging pick-up unit, is characterized in that the length of described cuboid three adjacent edges is 15cm, 15cm and 40cm respectively.

6. Portable fluorescence according to claim 1 and chemiluminescence imaging pick-up unit, is characterized in that described cabinet being also provided with fan 9 and usb 11.

7. Portable fluorescence according to claim 6 and chemiluminescence imaging pick-up unit, be is characterized in that described usb 11 wire connects camera, is connected, and carries out image acquisition control and graphical analysis by computing machine by usb 11 with computing machine.

8. Portable fluorescence according to claim 1 and chemiluminescence imaging pick-up unit, is characterized in that described cabinet is provided with handle.

9. Portable fluorescence according to claim 1 and chemiluminescence imaging pick-up unit, is characterized in that described refrigeration CCD camera lens has the EPI pallet of the black of Teflon coating.

10. Portable fluorescence according to claim 1 and chemiluminescence imaging pick-up unit, is characterized in that described excitation source is ultraviolet, red, green or blue excitation source.