CN204429320U - A kind of isostasy declines fluid chip fixator - Google Patents

Abstract

The utility model discloses a pressure-balanced microfluidic chip holder, which comprises a base. The base is recessed from the upper surface to the middle of the base to form a concave cavity, and a chip carrier for placing chips is arranged in the concave cavity; A transparent cover body that can be covered on the concave cavity, and a tube hole is opened on the cover body; a rotary table for pushing the slide table is installed at the bottom of the concave cavity. The utility model adopts a transparent cover body, which can observe the position and angle of the chip during the fixing process, reduce chip damage caused by improper operation, and at the same time correct events such as liquid leakage and dislocation of the swimming lane in time; adopt spiral propulsion and The method of surface contact makes the pressure distribution more uniform during the chip fixing process, and overcomes the defect of chip damage caused by uneven local force in the prior art; it is easy to disassemble and fix, and it can be placed only by opening the fixing bolt , Take out the chip, the alignment process is faster and more convenient.

Description

A pressure-balanced microfluidic chip holder

technical field

The utility model relates to an auxiliary experiment appliance, in particular to a pressure-balanced microfluidic chip holder.

Background technique

The microfluidic chip is characterized by the structure of the micropipeline network. It adopts the micro-electromechanical processing technology similar to semiconductors to construct the micro-fluidic system on the chip, and transfers the experiment and analysis process to the interconnected flow path and micro-control components. On the chip composed of micro-sample, it realizes a micro-total analysis experimental device integrating micro-sample preparation, sample injection, reaction, separation and detection. Microfluidic chip technology can not only increase the analysis speed, improve the analysis efficiency, greatly reduce the consumption of samples and reagents, but also automate the analysis process, reduce human interference and pollution, and complete experiments efficiently. It is widely used in cell screening and information ribonucleic acid extraction. and purification, gene sequencing, single cell analysis, protein crystallization, drug monitoring, etc.

The PLGA chip is a microfluidic membrane chip that can be used to capture specific cells after specific modification. During the experiment, the TOP chip that acts as a guide is used to establish a microfluidic channel on the chip, which is precisely controlled by an automatic sampling device. The cell suspension passes through the channel, and the modified material on the chip is used to specifically capture the specific cells. This kind of membrane chip can realize the separation of specific cells by laser capture microdissection technology, so as to facilitate subsequent related molecular biology experiments, which has far-reaching significance for both basic and clinical research.

At present, the problem in this experiment is that when conducting cell suspension diversion, it is necessary to fix the chip and press it tightly with the pressure sheet so that the channel is independent and prevent leakage during the diversion suspension process, but the existing The pressure distribution of advanced experimental equipment is uneven during the fixing process, which may easily lead to chip damage or liquid leakage in the swim lane.

Contents of the invention

In view of the above-mentioned problems in the prior art, the purpose of this utility model is to provide a pressure-balanced microfluidic chip holder, which solves the defects of the existing holder that easily cause chip damage and cannot observe the fixing situation.

In order to achieve the above tasks, the utility model adopts the following technical solutions:

A pressure-balanced microfluidic chip holder, including a base, the base is recessed from the upper surface to the middle of the base to form a concave cavity, and a slide table for placing chips is arranged in the concave cavity; The transparent cover body on the cavity is provided with tube holes; the bottom of the concave cavity is equipped with a rotary table for pushing the slide table.

Further, the turntable is in the shape of a round cake, the top surface of the turntable is fixed with a push platform, and the outer surface of the push platform is provided with external threads; the bottom of the concave cavity is provided with a through hole, and the through hole is provided with a The internal thread mating with the above external thread.

Further, a pair of first fixed platforms and a pair of second fixed platforms are respectively provided on both sides of the top of the base, wherein a rotating shaft is arranged between the first fixed platforms, and fixed holes are symmetrically opened on the second fixed platforms, and the One end of the cover body is installed on the rotating shaft, and the other end is fastened through the fixing hole on the second fixing platform.

Further, the cover body is a rectangular cover body, the first side plate and the second side plate are fixed at both ends of the cover body respectively, and the first side plate and the second side plate are provided with mounting brackets along the direction parallel to the base. Holes, wherein the mounting hole of the second side plate is sleeved on the rotating shaft, and after the mounting hole of the first side plate is aligned with the fixing hole on the first fixing platform, it is fixed by a fixing bolt.

Further, the described slide table has a rectangular structure and is placed in the concave cavity; the upper surface of the slide table is provided with a second draw-in groove along the length direction, and a first draw-in groove along the width direction, and the depth of the two draw-in grooves is identical and partially overlapping.

Further, one end of the fixing bolt is provided with an anti-slip post.

Further, the base is a rectangular structure.

Compared with the prior art, the utility model has the following technical characteristics:

1. With the transparent cover, the position and angle of the chip during the fixing process can be observed, and the chip damage caused by improper operation can be reduced;

2. It is easy to disassemble and assemble, and the fixing is firm. The chip can be placed and taken out only by opening the fixing bolt, and the alignment process is faster and more convenient;

3. Adopt the method of spiral propulsion and surface contact to make the pressure distribution more uniform during the chip fixing process, and overcome the defect of chip damage caused by local uneven force in the prior art;

4. The degree of compression is easy to adjust, with strong controllability, and can be adjusted under intuitive observation, which improves the efficiency of the experiment;

5. The overall structure of the device is simple, the production cost is low, and it is convenient for later disinfection and storage.

Description of drawings

Fig. 1 is the overall structural representation of the utility model;

Fig. 2 is the exploded view of parts of the utility model;

The symbols in the figure represent: 1—base, 11—the first fixed platform, 12—the second fixed platform, 13—rotating shaft, 14—fixed hole, 15—internal thread, 16—cavity, 2—cover, 21—the first Two side plates, 22—first side plate, 23—pipe hole, 3—carrier table, 31—first card slot, 32—second card slot, 4—rotary table, 41—push table, 42—external thread , 5—anti-slip column, 51—fixed bolt;

Detailed ways

Good fixation of the microfluidic chip is the prerequisite to ensure the smooth progress of the experiment. When using a PLGA chip to capture cells, it is first necessary to attach a silica gel sheet with a fluid channel on the lower surface to the chip, so that a fluid channel with the upper surface of the chip as the bottom surface is formed on the modified material on the chip surface. The round hole on the silica gel sheet is used to fix the position of the sampling thin tube. The upper thin tube is connected to the automatic sampling device to precisely control the fluid volume and flow rate, so that the cell suspension can flow smoothly through the fluid channel, and the lower end thin tube is connected to the waste liquid collection. device. Taking the cell suspension as an example, the cell suspension is controlled by the autosampler and flows into the chip lane through the upper capillary tube, in which the cells that can be combined with the modified material on the chip are specifically recognized and captured, while the unrecognized and captured cells will be captured along with them. The liquid flows out through the lower thin tube. After the experiment is over, inject a small amount of air into the channel with the autosampler to facilitate the separation of the membrane chip from the silica gel pad on it. Disassemble the device to take out the membrane chip, peel off the upper silica gel pad, and the membrane chip enriched with target cells can be dried and stored in a -80° refrigerator or cut and collected by laser capture microdissection for subsequent molecular biology. related experiment process.

Because the chip is fragile and thin, it is very easy to be broken and damaged during operation. Therefore, when fixing the chip, it is necessary to pay attention to the uniform distribution of the fixing pressure; at the same time, the whole fixing process and the experimental process should be observed at any time. In this solution, a pressure-balanced microfluidic chip holder is proposed, including a base 1, the base 1 is recessed from the upper surface to the middle of the base 1 to form a cavity 16, and a slide table 3 for placing chips is arranged in the cavity 16 ; The base 1 is equipped with a transparent cover 2 that can be covered on the cavity 16, and the cover 2 is provided with a tube hole 23;

The transparent cover 2 proposed in this solution can visually observe the situation inside the concave cavity 16. When the chip is fixed, if the position deviation, bending, etc., can be corrected in time, and if leakage occurs during the experiment can also intervene in a timely manner. A pair of tube holes 23 are arranged on the cover body 2 , the upper tube holes 23 are connected with an automatic sampling needle through a hose to control the introduction of cell suspension, and the lower tube holes 23 are used to export excess liquid through a hose. The position of the tube hole 23 corresponds exactly to the position of the circular hole on the above-mentioned silica gel sheet, so that the suspension enters the circular hole from the tube hole 23 in turn, and then enters the microfluidic channel.

The function of the cavity 16 on the base 1 is to accommodate the slide table 3 so as to facilitate the movement of the slide table 3 . The idea of fixation in this solution is to continuously reduce the distance between the loading table 3 and the inner surface of the cover body 2, so that the chips arranged on the surface of the loading table 3 are compressed and fixed. This requires that the inner surface of the cover body 2 and the upper surface of the slide table 3 be flat and smooth.

The rotary table 4 is used to push the slide table 3 to move, and the rotary table 4 can push the slide table 3 in various ways, for example, in a rotating manner. The pushing process should be stable and evenly stressed.

This scheme proposes a structure of a turntable 4, the turntable 4 is round cake shape, the top surface of the turntable 4 is consolidated with a push platform 41, and the outer surface of the push platform 41 is provided with an external thread 42; the bottom of the concave cavity 16 A through hole is opened, and the internal thread 15 matched with the external thread 42 is arranged in the through hole.

The upper surface of the pushing platform 41 is plane, and the upper surface is in contact with the bottom surface of the slide table 3 arranged in the cavity 16, and the bottom surface of the slide table 3 is also plane. When turning the turntable 4, due to the cooperation of the external thread 42 and the internal thread 15, the push platform 41 on the turntable 4 pushes the slide table 3 to move in a direction close to the cover body 2, and the propulsion force is effectively dispersed due to surface contact , so that the slide table 3 can move smoothly, so that the chips and pressing tablets on the slide table 3 can stably contact the inner surface of the cover body 2, and then be pressed tightly without uneven force.

The specific setting structure of base 1 is:

A pair of first fixed platforms 11 and a pair of second fixed platforms 12 are respectively provided on both sides of the top of the base 1, wherein a rotating shaft 13 is arranged between the first fixed platforms 11, and fixed holes 14 are symmetrically opened on the second fixed platforms 12, so that One end of the above-mentioned cover body 2 is installed on the rotating shaft 13 , and the other end is fastened through the fixing hole 14 on the second fixing table 12 .

As shown in Figure 1, the base 1 is a rectangular structure, the concave cavity 16 is set inside the base 1 and penetrates with the upper surface, and two pairs of fixing platforms are arranged on the upper surface of the base 1 at the edge of the concave cavity 16 for installing the cover body 2 . The first fixed table 11 is connected by a rotating shaft 13, one end of the cover body 2 is installed on the rotating shaft 13, and the other end can be buckled on the base 1 in an openable and closable manner to block the concave cavity 16, and this end can be fixed by the second Fixing holes 14 on the platform 12 are fastened.

The cover body 2 is a rectangular cover body 2, and the two ends of the cover body 2 are respectively fixed with a first side plate 22 and a second side plate 21, and the first side plate 22 and the second side plate 21 are respectively opened along the direction parallel to the base 1. There are installation holes, wherein the installation hole of the second side plate 21 is sleeved on the rotating shaft 13, and after the installation hole of the first side plate 22 is aligned with the fixing hole 14 on the first fixing table 11, it is fixed by a fixing bolt 51.

Corresponding to the base 1, the cover body 2 is also arranged in a rectangular structure, and the two side plates provided at its two ends are respectively used for corresponding installation with the fixed platform. The mounting hole on the second side plate 21 is passed by the rotating shaft 13, so that the second side plate 21 is hinged on the second fixed platform 12, and then the first cover 2 is covered on the concave cavity 16. At this time, the cover 2 It is parallel to the base 1 , and the first side plate 22 is located between the two first fixing platforms 11 , and the positions of the mounting holes and the fixing holes 14 exactly correspond, that is, the axes are collinear. At this time, a fixing bolt 51 is used to pass through the installation hole and the fixing hole 14 , and the cover body 2 is fastened on the surface of the base 1 .

One end of the fixing bolt 51 is provided with an anti-slip column 5, the diameter of this part is larger than the diameter of the fixing bolt 51, and the anti-skid pattern is engraved on it, which is convenient for taking and fixing simultaneously.

The slide table 3 has a rectangular structure and is placed in the cavity 16; the upper surface of the slide table 3 is provided with a second slot 32 along the length direction, and a first slot 31 along the width direction, and the two slots have the same depth and partially overlap.

The slide table 3 is used to place chips and silica gel sheets. Two card slots are arranged alternately on the slide table 3, which can correspond to chips of different sizes. Placing the chips in the card slots can prevent the chips from shaking horizontally . And the slide table 3 is also arranged in a rectangular structure, which can prevent the situation of position deviation due to rotation during its movement; in addition, the slide table 3 shown in FIG.

Claims (7)

1. an isostasy declines fluid chip fixator, comprise base (1), it is characterized in that, described base (1) forms cavity (16) by upper surface to base (1) medial recess, is provided with the slide holder (3) for chip placement in cavity (16); Base (1) is provided with the transparent lid (2) that can cover on cavity (16), lid (2) offers pore (23); Cavity (16) bottom is provided with revolves platform (4) for what promote slide holder (3).

2. isostasy as claimed in claim 1 declines fluid chip fixator, it is characterized in that, the described platform (4) that revolves is round pie, revolve platform (4) end face to be consolidated with and to push away platform (41), push away platform (41) outer surface and be provided with external screw thread (42); Described cavity (16) bottom offers through hole, is provided with the internal thread (15) coordinated with described external screw thread (42) in through hole.

3. isostasy as claimed in claim 1 declines fluid chip fixator, it is characterized in that, described base (1) both sides, top are respectively arranged with a pair first fixed stations (11) and a pair second fixed stations (12), wherein be provided with rotating shaft (13) between the first fixed station (11), the upper symmetry of second fixed station (12) offers fixing hole (14), described lid (2) one end is arranged in rotating shaft (13), and the other end is fastening by the fixing hole (14) on the second fixed station (12).

4. isostasy as claimed in claim 3 declines fluid chip fixator, it is characterized in that, described lid (2) is rectangle lid, lid (2) two ends are consolidated with the first side plate (22) and the second side plate (21) respectively, first side plate (22) and the upper edge of the second side plate (21) are parallel to base (1) direction and all offer installing hole, wherein the installing hole of the second side plate (21) is sleeved in rotating shaft (13), after the installing hole of the first side plate (22) is aimed at the fixing hole (14) on the first fixed station (11), be fixed by a gim peg (51).

5. isostasy as claimed in claim 1 declines fluid chip fixator, and it is characterized in that, described slide holder (3) is rectangular configuration, is placed in cavity (16); The upper surface of slide holder (3) is provided with the second draw-in groove (32) along its length, is provided with the first draw-in groove (31) in the width direction, and two draw-in groove degree of depth are identical and partially overlap.

6. isostasy as claimed in claim 3 declines fluid chip fixator, and it is characterized in that, one end of described gim peg (51) is provided with cleat (5).

7. isostasy as claimed in claim 1 declines fluid chip fixator, and it is characterized in that, described base (1) is rectangular configuration.