CN204325370U - Hand-held instant detection device - Google Patents

Abstract

The utility model discloses a handheld instant detection device, which includes a body, a cover and a detection unit. The body has an opening and a base. The cover body has a control unit and a sealing unit. The sealing unit is arranged on the opening of the body. The detection unit is arranged on the base inside the body and has a temperature controller, a light exciter, a light detector and a circuit board. The thermostat is disposed on one side of the opening and has at least one temperature control area. The light exciter is arranged between the thermostat and the base. The light detector is arranged between the thermostat and the opening. The circuit board is electrically connected to the control unit, thermostat, light exciter and light detector respectively.

Description

Handheld point-of-care testing device

technical field

The utility model relates to a detection device, in particular to a hand-held instant detection device.

Background technique

Polymerase Chain Reaction (PCR) is a technique widely used in molecular diagnostics today. Almost all commercially available PCR systems use a thermocycler to heat and cool down repeatedly to make deoxyribonucleic acid ( Deoxyribonucleic Acid, DNA) undergoes three different temperature cycles of denaturation (95 degrees Celsius), bonding (45-65 degrees Celsius) and extension (72 degrees Celsius), and the heat energy is gradually conducted (thermal conduction) into the reaction test tube through the metal block , and maintain an appropriate response time. However, with such a multi-level indirect heating method, most of the time is spent on the heating and cooling of the metal block, the reaction takes a long time, and the machine is bulky.

In addition, in recent years, many researches on microchannel PCR chips have been proposed one after another, integrating microchannels and microstructures, so that the reaction process, such as various sample processing, reaction or analysis and detection, can be realized on the same laboratory chip. On a chip (LOC), the advantage of LOC is that the size can be miniaturized, and fast temperature control, low sample volume, and cost reduction can all be realized. So far, although a lot of research has been put forward, there is no product that has been successfully launched. The main problem is that the reproducibility is not high enough. In addition, a liquid drive system is required, so there is still a certain complexity and development cost, which limits the application range.

One of the temperature setting method and device design of the currently known PCR reaction is to place a test tube containing a mixed solution and a DNA sample in a closed casing, and use a heater to heat the mixed solution at the bottom of the test tube To the denaturation temperature, the mixed solution in the test tube will generate thermal convection due to the temperature gradient, so that the temperature of the mixed solution will be continuously changed during the convection process, and the PCR reaction can be carried out in a circle with the mixed solution convection. The key point of its patent is the method and device for temperature setting, which has the advantages of simple structure and low cost, which can shorten the time required for the traditional method to change the temperature and is applied to the environment of a single heating source.

Still another design is to propose a special reaction test tube for nucleic acid amplification reaction, the main body is a plastic capillary, and the bottom is tightly wrapped with a metal conductor ring. Put this test tube on a heating block, because the heating block is in direct contact with the metal ring, and the metal ring tightly wraps the bottom of the plastic capillary, so the heat can be transferred to the liquid in the plastic capillary, keeping the bottom at 95 degrees Celsius; the upper part of the capillary The lid can be open, or maintained at 50 degrees Celsius with a temperature control device, so that a temperature gradient of hot bottom and cold top is formed, so that the PCR reagents in the tube can be amplified through continuous temperature cycles. The focus of this patent is to advocate the design concept and application of the PCR test tube with a heat conduction ring in the environment of a single heating source.

Utility model content

The purpose of this utility model is to propose a hand-held real-time detection device, which can have the convenience of hand-held, quickly meet the requirements of the entire PCR reaction process and immediately return the detection results to the touch panel interface, which is convenient for users to directly Control and read measurement data instantly.

To achieve the above purpose, the utility model proposes a hand-held real-time detection device, which includes a body, a cover and a detection unit. The body has an opening and a base. The cover has a control unit and a sealing unit. The sealing unit is arranged on the opening of the body. The detection unit is arranged on the base inside the body and has a temperature controller, a light exciter, a light detector and a circuit board. The temperature controller is arranged on one side of the opening and has at least one temperature control area. The light exciter is arranged between the temperature controller and the base. The light detector is arranged between the temperature controller and the opening. The circuit board is electrically connected to the control unit, the temperature controller, the light exciter and the light detector respectively. Among them, the control unit respectively sends a temperature control instruction, an excitation instruction and a detection instruction through the circuit board, and then transmits the temperature control instructions to the temperature controller to adjust the temperature of the temperature control area, and transmits the excitation instruction to the optical excitation. The device is used to excite and irradiate the temperature-controlled area, and transmit the detection instruction to the photodetector to detect the temperature-controlled area in real time, and the photodetector transmits a detected detection signal to the The control unit displays a detection result in real time.

The handheld instant detection device also includes at least one detection test tube disposed on the temperature control area of the temperature controller.

The thermostat adjusts the temperature of an analyte in the test tube to generate heat convection, the light exciter irradiates the analyte, and the photodetector detects the analyte The detection signal is generated by exciting the light source.

The handheld instant detection device also includes a cooling fan.

The control unit is a touch panel interface or a mechanical button interface.

The close unit is made of Bakelite.

This thermostat has heating function or cooling function.

The thermostat is a heating patch.

The light source of the light exciter is a light emitting diode.

The photodetector is a photodiode, a charge coupled device (CCD) or a complementary metal oxide semiconductor (CMOS).

The utility model has the advantage that the hand-held instant detection device designed by the utility model is different from the temperature changing method of the traditional thermal cycler, and uses an adjustable constant temperature control to locally heat the detection test tube, and cooperates with a detection unit designed by a special mechanism , which can make the analyte in the test tube produce a stable temperature gradient. When used in PCR detection, the temperature is adjusted at the bottom of the test tube to provide high temperature for denaturation reaction, and the temperature gradient from the bottom of the test tube to the surface is gradually reduced to induce thermal convection, covering denaturation (95 degrees Celsius), adhesion (45-65 degrees Celsius) degrees) and extension (72 degrees Celsius) three different temperature cycles. If the nucleic acid amplification reagent is contained in the detection test tube, heat convection circulation can be naturally generated, and the reaction can be automatically completed through different temperature regions, which can simplify the temperature control module of the handheld instant detection device of the present invention, and can also have the convenience of hand-held It can quickly meet the requirements of the entire PCR reaction process and immediately send the test results back to the touch panel interface, which is convenient for users to directly control and read the measurement data in real time.

Description of drawings

Fig. 1 is the appearance schematic diagram of the hand-held real-time detection device of the utility model embodiment;

Fig. 2 is the internal schematic diagram of the hand-held real-time detection device of the utility model embodiment;

Fig. 3 is the schematic diagram of the detection unit of the utility model embodiment;

Fig. 4 is the internal schematic diagram of the hand-held instant detection device with detection test tube in the embodiment of the utility model;

Fig. 5 is a schematic diagram of a detection unit with a detection test tube according to an embodiment of the present invention.

Symbol Description

10-Handheld real-time detection device

100-body

102-opening

104-base

110-cover body

112 - control unit

114-closed unit

120-detection unit

122-thermostat

123-Temperature control area

124-photo exciter

126-light detector

128-circuit board

130-cooling fan

140-test tube

142-tube cover

Detailed ways

Please refer to Figures 1 to 3 at the same time, Figure 1 is a schematic diagram of the appearance of the handheld real-time detection device of the embodiment of the utility model, Figure 2 is a schematic diagram of the interior of the handheld real-time detection device of the embodiment of the utility model, and Figure 3 is a schematic diagram of the implementation of the utility model Schematic diagram of the detection unit of the example. The handheld real-time testing device 10 includes a main body 100 , a cover 110 and a testing unit 120 . The body 100 has an opening 102 and a base 104 . The cover 110 has a control unit 112 and a sealing unit 114 . The sealing unit 114 is disposed on the opening 102 of the main body 100. In this embodiment, one end of the cover 110 is fixed on the opening 102 of the main body 100, and the cover 110 can be rotated to open or close on the opening 102 of the main body 100. , but not limited to this. The control unit 112 can be a touch panel interface or a mechanical button interface, and the sealing unit 114 can be a bakelite material with low thermal conductivity, but not limited thereto. The detection unit 120 is disposed on the base 104 inside the main body 100 and has a temperature controller 122 , a light exciter 124 , a light detector 126 and a circuit board 128 . The thermostat 122 is arranged on one side of the opening 102 and has at least one temperature control area 123, wherein the thermostat 122 can have a heating function or a cooling function, or the thermostat 122 can also be a heating patch, but not with This is the limit. The photo-stimulator 124 is disposed between the thermostat 122 and the base 104 , wherein the light-emitting source of the photo-stimulator 124 can be a light-emitting diode, but not limited thereto. The photodetector 126 is disposed between the temperature controller 122 and the opening 102, wherein the photodetector 126 can be a photodiode (photodiode), a photosensitive coupling device (Charge Coupled Device, CCD) or a complementary metal oxide semiconductor (Complementary Metal-Oxide Semiconductor, CMOS), but not limited thereto. The circuit board 128 is electrically connected to the control unit 112 , the temperature controller 122 , the light exciter 124 and the light detector 126 respectively.

Wherein, the control unit 112 respectively sends a temperature control instruction, a heat dissipation instruction, an excitation instruction and a detection instruction through the circuit board 128, and then transmits the temperature control instructions to the thermostat 122 to adjust the temperature of the temperature control area 123. , transmit the heat dissipation instruction to the heat dissipation fan 130 to adjust the heat dissipation of the temperature control area 123, transmit the excitation instruction to the light exciter 124 to excite and irradiate the temperature control area 123, and transmit the detection instruction to the light detector 126 The light detector 126 transmits a detected detection signal to the control unit 112 via the circuit board 128 to immediately display a detection result to detect the temperature control area 123 by exciting the light source immediately. In this embodiment, the handheld instant detection device 10 also includes a heat dissipation fan 130 to dissipate heat from the detection unit 120, but it is not limited thereto; It can be a circular symmetrical design, and a cavity is left around the side wall between the circular symmetrical design of each element of the detection unit 120 and the main body 100, and there is a ventilation hole in the center of the base 104, which is matched with the one side of the base 104. The cooling fan 130 allows the detection unit 120 to achieve fluid circulation, thereby helping to stabilize the overall detection temperature; the cover 110 is combined with the control unit 112 with a touch panel interface, which is convenient for the user to directly control and read the measurement data in real time, but does not use This is the limit.

Please refer to FIG. 4 to FIG. 5 at the same time. FIG. 4 is a schematic diagram of the interior of the hand-held real-time detection device with a test tube according to an embodiment of the present invention, and FIG. 5 is a schematic diagram of a detection unit with a test tube according to an embodiment of the present invention. The handheld instant detection device 10 also includes at least one detection test tube 140 disposed on the temperature control area 123 of the temperature controller 122 . Among them, the temperature controller 122 adjusts the temperature of a test object in the detection test tube 140 to generate a heat convection effect, the optical exciter 124 excites and irradiates the test object, and the light detector 126 detects the heat generated by the test object. The detection signal of the excitation light source. Wherein when the sealing unit 114 of the cover body 110 is closed on the opening 102 of the body 100, the sealing unit 114 will press and fix the tube cover 142 of the detection test tube 140 and achieve heat preservation for the interior of the handheld instant detection device 10. effect.

To sum up, the handheld instant detection device designed by the utility model is different from the temperature changing method of the traditional thermal cycler, and uses an adjustable constant temperature control to locally heat the detection test tube, and cooperates with the detection unit designed by a special mechanism. Make the analyte in the test tube produce a stable temperature gradient. When used in PCR detection, the temperature is adjusted at the bottom of the test tube to provide high temperature for denaturation reaction, and the temperature gradient from the bottom of the test tube to the surface is gradually reduced to induce thermal convection, covering denaturation (95 degrees Celsius), adhesion (45-65 degrees Celsius) degrees) and extension (72 degrees Celsius) three different temperature cycles. If the nucleic acid amplification reagent is contained in the detection test tube, heat convection circulation can be naturally generated, and the reaction can be automatically completed through different temperature regions, which can simplify the temperature control module of the handheld instant detection device of the present invention, and can also have the convenience of hand-held It can quickly meet the requirements of the entire PCR reaction process and immediately send the test results back to the touch panel interface, which is convenient for users to directly control and read the measurement data in real time.

Claims (10)

1. the instant proofing unit of hand-held, is characterized in that, the instant proofing unit of this hand-held comprises:

Body, it has opening and base;

Lid, it has:

Control unit; And

Closely sealed unit, it is arranged on this opening of this body; And

Detecting unit, it to be arranged on this this base intrinsic and to have:

Temperature controller, it is arranged at the side of this opening and has at least one temperature control region;

Optical excitation device, it is arranged between this temperature controller and this base;

Optical detector, it is arranged between this temperature controller and this opening; And

Circuit card, it is electrically connected at this control unit, this temperature controller, this optical excitation device and this optical detector respectively;

Wherein, this control unit sends a temperature control instruction, respectively and excites instruction to detect indicate via this circuit card with one, respectively this temperature control instruction is sent to this temperature controller to carry out temperature adjustment to this temperature control region again, instruction this is excited to be sent to this optical excitation device to carry out exciting irradiation to this temperature control region, be sent to this optical detector to carry out instant excitation light source detecting to this temperature control region with this detecting being indicated, a detection signal of detecting is sent to this control unit via this circuit card and detects result with instant playback one by this optical detector.

2. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, the instant proofing unit of this hand-held also comprises at least one test tubes and is arranged on this temperature control region of this temperature controller.

3. the instant proofing unit of hand-held as claimed in claim 2, it is characterized in that, this temperature controller carries out temperature to the determinand in this test tubes and regulates to produce a thermal convection effect, this optical excitation device carries out exciting irradiation to this determinand, this detection signal of the excitation light source that this determinand of detecting produces by this optical detector.

4. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, the instant proofing unit of this hand-held also comprises radiator fan.

5. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, this control unit is a contact panel interface or a mechanical botton interface.

6. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, this closely sealed unit is a bakelite material.

7. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, this temperature controller has heating function or refrigerating function.

8. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, this temperature controller is a heat patch.

9. the instant proofing unit of hand-held as claimed in claim 1, it is characterized in that, the luminous source of this optical excitation device is a photodiode.

10. the instant proofing unit of hand-held as claimed in claim 1, is characterized in that, this optical detector is a photorectifier or a photosensitive coupling element or a Complimentary Metal-Oxide semi-conductor.