CN204293134U - A kind of laser nano optics diagnostic equipment - Google Patents

Abstract

The utility model provides a laser nano-optical diagnosis and treatment equipment, which is characterized in that it comprises: a treatment darkroom; a carrying platform; a first laser, which is arranged in the treatment darkroom, and emits laser light to irradiate the treatment object; a charge-coupled image sensor CCD, It is arranged in the treatment darkroom, and is used to shoot the treatment object through a filter; the filter is arranged between the treatment object and the CCD; the rotating table is arranged in the treatment darkroom, connected to The second laser is adjusted by rotating the emission direction of the second laser to align with the area of the object to be treated that is rich in luminescence; the second laser is installed on the rotating table and is used to emit laser light The luminescence-enriched area in the body of the subject to be treated. The utility model monitors the metabolism, distribution and enrichment of the nano photosensitizer in real time, accurately locates the position of the tumor, and realizes non-invasive and non-invasive tumor treatment for the treatment object.

Description

A laser nano-optical diagnosis and treatment equipment

technical field

The utility model relates to the technical field of physical diagnosis and treatment, in particular to a laser nanometer optical diagnosis and treatment equipment.

Background technique

Traditional surgical resection, chemotherapy, radiotherapy or biological therapy have made extraordinary achievements in tumor treatment, but their toxic side effects, multidrug resistance and other problems are still difficult to overcome. In recent years, photothermal and photodynamic therapy of near-infrared light-activated nanomaterials that penetrate the skin have been increasingly favored due to their advantages such as non-invasive, non-toxic, targeted, and efficient.

Photothermal therapy refers to using the thermal effect of various heat sources to heat the tumor area or the whole body to an effective temperature for treatment and maintain it for a certain period of time. Using the difference in temperature tolerance between normal tissue and tumor tissue, it can kill both tumors and tumors. Treatment of tumor cells without damaging normal tissues.

Photodynamic therapy refers to the method of using photosensitizers to change the function or shape of organism cells or biomolecules under the action of light to achieve therapeutic effects. Photodynamic therapy is another brand-new therapy that is being researched and developed rapidly after surgery, radiotherapy, chemotherapy and immunotherapy. It has become one of the most active research fields in the world's tumor prevention and treatment science.

A large number of experiments have been carried out in photothermal and photodynamic therapy at home and abroad; at the same time, the emerging optical imaging technology in living animals has achieved a revolutionary leap, which can meet the requirements of medical ethics in animal experiments. Real-time optical imaging, Image-guided therapy has shown unique advantages in tumor treatment, but there is no tumor diagnosis and treatment equipment that integrates photothermal/photodynamic therapy and in vivo imaging.

Utility model content

The main purpose of the embodiment of the utility model is to provide a laser nano-optical diagnosis and treatment equipment, so as to provide a diagnosis and treatment equipment integrating photothermal treatment technology, photodynamic treatment technology and in vivo imaging technology.

In order to achieve the above purpose, the embodiment of the present invention provides a laser nano-optical diagnosis and treatment equipment, including:

treatment darkroom;

The carrying platform is set in the treatment darkroom and is used to carry the treatment object injected with the nano-photosensitizer in the body;

The first laser is set in the treatment darkroom, and is used to emit laser light to irradiate the treatment object, so that the nano-photosensitizer injected into the treatment object emits light;

A charge-coupled image sensor CCD is set in the treatment darkroom and is used to photograph the treatment object through a filter;

An optical filter, arranged between the subject to be treated and the CCD, is used to filter out the light of a set spectrum, so as to transmit the light emitted by the nano-photosensitizer;

The rotating table is set in the treatment dark room, connected with the second laser, and adjusts the emission direction of the second laser by rotating to align with the area of enriched luminescence in the body of the treatment object;

The second laser is installed on the rotating table, and is used to emit laser light to irradiate the luminescence-rich area in the body of the treatment object.

With the help of the above-mentioned technical scheme, the laser nano-optical diagnosis and treatment equipment provided by the utility model emits laser light to make the nano-photosensitizer injected into the treatment subject emit light, and monitors the metabolism, distribution and enrichment of the nano-photosensitizer in real time, so as to accurately locate the tumor By emitting laser light on the nano-photosensitizer in the tumor area to make it play the role of photothermal therapy or photodynamic therapy, it can realize non-invasive and non-invasive tumor treatment for the treatment object.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings that need to be used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only the present invention For some novel embodiments, those of ordinary skill in the art can also obtain other drawings based on these drawings without any creative work.

Fig. 1 is the structural representation of the laser nano-optical diagnosis and treatment equipment provided by the utility model;

Fig. 2 is a schematic structural diagram of the laser nano-optical diagnosis and treatment equipment provided by Embodiment 1 of the present utility model.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

It should be noted that the nano-photosensitizer used in this utility model is made of biocompatible macromolecules such as phospholipids, phospholipid polymers, polysaccharides, polypolypeptides, and albumin, and is surface-modified with ligands such as folic acid and Her2. Indocyanine green, IR780, porphyrin, nano-gold and other photosensitizers can be used to prepare degradable nano-photosensitizers, which can realize targeted recognition and enrichment of tumors, and have the advantages of good fluorescence stability and low toxicity and side effects.

Below is the preparation method of a kind of nano photosensitizer that the utility model uses: with indocyanine green, soybean lecithin and distearoylphosphatidylethanolamine-polyethylene glycol-folic acid, by mass ratio 1:1.7:1.7 dissolve In an organic solvent, the organic solvent is removed by rotary evaporation to obtain a film-like material. Add 2.5mL of ultrapure water, sonicate for 5 minutes with an ultrasonic breaker, and wash with ultrafiltration membrane for 3 times to obtain the targeted nano-photosensitizer loaded with indocyanine green phospholipid folic acid.

The utility model provides a laser nano-optical diagnosis and treatment equipment, as shown in Figure 1, the equipment comprises: a treatment darkroom 101, a carrying platform 102, a first laser 103, a charge-coupled image sensor CCD (Charge-coupled Device) 104, a light filter Sheet 105, rotary table 106, second laser 107.

During specific implementation, the treatment darkroom 101 may be made of plastic, metal, wood or stone.

The carrying platform 102 is set in the treatment darkroom 101 and is used for carrying the treatment object injected with the nano photosensitizer in the body.

The first laser 103 is arranged in the treatment dark room 101, and is used to emit laser light to irradiate the treatment object, so as to make the nano-photosensitizer injected into the treatment object emit light.

The CCD 104 is set in the treatment dark room 101 .

The optical filter 105 is arranged between the subject to be treated and the CCD 104, and is used to filter out the light of a set spectrum, and to transmit the light emitted by the nano-photosensitizer.

In the present invention, the optical filter 105 can achieve the purpose of filtering stray light and improve the accuracy of locating the tumor position according to the light emitted by the nano photosensitizer. During specific implementation, the optical filter 105 needs to cooperate with the nano-photosensitizer injected into the treatment object, so as to achieve the purpose of passing the light emitted by the nano-photosensitizer and filtering out stray light.

The CCD104 is used to photograph the treatment object through the optical filter 105.

During specific implementation, the nano-photosensitizer can realize the targeted recognition and enrichment of the tumor, so the area where the luminescence is enriched in the image of the treated subject is the tumor area.

The rotating table 106 is set in the treatment darkroom 101, connected with the second laser 107, and adjusts the emission direction of the second laser 107 by rotating to align with the area in the body of the treatment subject that is rich in light (ie, the tumor area).

The second laser 107 is installed on the rotating table 106, and is used to emit laser light to treat the area rich in luminescence in the body of the subject, so that the nano-photosensitizer can exert photothermal therapy and/or photodynamic therapy.

Specifically, the wavelength and power of the laser light emitted by the first laser 103 need to match the nano-photosensitizer injected into the subject's body to achieve the purpose of making it emit light. Preferably, the wavelength range of the laser light emitted by the first laser 103 is 420-800 nanometers.

Specifically, the wavelength and power of the laser light emitted by the second laser 107 also needs to match the nano-photosensitizer injected into the body of the treatment object, so as to achieve the purpose of making it exert the effect of photothermal therapy or photodynamic therapy. Preferably, the wavelength range of the laser light emitted by the second laser 107 is 600-1400 nanometers.

In a preferred embodiment, the laser nano-optic diagnosis and treatment equipment shown in Fig. 1 can also include an infrared thermal imager, which is composed of an infrared probe, an infrared thermal image processor and a thermal image display; wherein , the infrared probe is set in the treatment darkroom, used to receive the infrared rays emitted by the treatment object; the infrared thermal image processor is connected to the infrared probe, and is used to generate a corresponding temperature distribution image according to the infrared rays emitted by the treatment object ; The thermal imaging display is connected to the infrared thermal imaging processor to display the temperature distribution image. In this way, relevant medical personnel can observe the local or overall temperature of the treatment object in real time through the thermal image display, which is conducive to timely adjustment of the treatment process to improve the treatment effect.

In another preferred embodiment, the laser nano-optical diagnosis and treatment equipment shown in Fig. 1 can also include an oximeter; the oximeter is made up of a monitoring probe and a data display; wherein, the monitoring probe is arranged on the treatment The dark room is connected to the treatment subject for measuring the blood oxygen concentration in the tumor area; the data display is connected to the monitoring probe and used for displaying the blood oxygen concentration data measured by the monitoring probe. In this way, relevant medical personnel can monitor the change of blood oxygen concentration in the tumor area through the data display, which is conducive to grasping the therapeutic effect in time and timely adjusting the treatment process and treatment time.

In a preferred embodiment, the laser nano-optical diagnosis and treatment equipment shown in Fig. 1 can also be provided with an illumination light source in the treatment darkroom for illuminating the treatment darkroom, so as to position various instruments in the darkroom. Adjustments, such as aiming the laser at the treatment target or tumor area.

During specific implementation, the illumination light source may be a circle of LED lamps evenly distributed in the treatment dark room.

Embodiment one

As shown in Figure 2, the present embodiment is a specific laser nano-optical diagnosis and treatment equipment, which includes: a treatment darkroom 201, a carrying platform 202, a first laser 203, a CCD 204, an optical filter 205, a main control circuit board 206, a rotating Taiwan 207, second laser 208, infrared thermal imager, oximeter, lighting source; wherein, the infrared thermal imager includes: infrared probe 209, infrared thermal image processor 210 and thermal image display 211; the oximeter includes: monitoring The probe 212 and the data display 213; the illumination light source includes: an LED lamp 214.

Wherein, the main control circuit board 207 includes: a first laser control circuit, a second laser control circuit, a turntable drive circuit, an illumination control circuit, and a CCD control circuit.

The first laser control circuit is connected to the first laser 203 through an optical fiber, and controls the first laser 203 to emit laser light with a set wave field and power, so that the nano-photosensitizer injected into the treatment object emits light.

The second laser control circuit is connected to the second laser 208 through an optical fiber, and controls the second laser 208 to emit a laser with a set wave field and power, so that the nano-photosensitizer injected into the body of the treatment object can exert photothermal therapy and/or photodynamic therapy. .

The rotating table drive circuit is connected to the rotating table 207 to control the rotation of the rotating table 207 to adjust the emitting direction of the second laser 208 .

The lighting control circuit is connected to the lighting source through wires to control the opening and closing of the lighting source.

The CCD control circuit is connected to the CCD204 through the data line to control the start and stop of the CCD204.

The rotating table drive circuit controls the rotation of the rotating table 207, so that the second laser 208 emits laser light at the area rich in luminescence (ie, the tumor area) in the body of the treatment object.

Embodiment two

In this embodiment, the laser nano-optical diagnosis and treatment equipment shown in FIG. 2 is used to perform photothermal therapy on mice.

The specific operation process is as follows: after the mice are anesthetized, inject 200 μL of 200 μg/mL indocyanine green phospholipid folic acid targeting nano-photosensitizer into the tail vein, and place the anesthetized mice on the supporting platform. The first laser emits 704nm laser light to irradiate the mice, the corresponding wavelength of the filter is 735nm, and the CCD takes pictures of the mice; after the nano-photosensitizer reaches the maximum enrichment in the tumor, the tumor area is determined; the second laser is turned on to emit 808nm The laser, whose power is adjusted to 1W/m ² , is irradiated on the tumor area; the temperature changes of the mouse and the tumor location are observed by an infrared thermal imager.

Embodiment Three

In this embodiment, the laser nano-optical diagnosis and treatment equipment shown in FIG. 2 is used to perform photodynamic therapy on mice.

The specific operation process is as follows: after the mice are anesthetized, inject 200 μL of 200 μg/mL indocyanine green phospholipid folic acid targeting nano-photosensitizer into the tail vein, and place the anesthetized mice on the supporting platform. The first laser emits 704nm laser light to irradiate the mice, the corresponding wavelength of the filter is 735nm, and the CCD takes pictures of the mice; after the nano-photosensitizer reaches the maximum enrichment in the tumor, the tumor area is determined; the second laser is turned on to emit 670nm The laser, whose power is adjusted to 50mW/m ² , is irradiated on the tumor area; the change of the blood oxygen concentration at the tumor site of the mice is observed by the oximeter.

The laser nano-optical diagnosis and treatment equipment provided by the utility model emits laser light to make the nano-photosensitizer injected into the treatment object emit light, and monitors the metabolism, distribution and enrichment of the nano-photosensitizer in real time, thereby accurately locating the position of the tumor. The nano-photosensitizer in the area emits laser light to make it play the role of photothermal therapy or photodynamic therapy, so as to realize non-invasive and non-invasive tumor treatment for the treated object.

The specific embodiments described above further describe the purpose, technical solutions and beneficial effects of the present utility model in detail. Within the protection scope of the utility model, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the utility model shall be included in the protection scope of the utility model.

Claims (6)

1. a laser nano optics diagnostic equipment, is characterized in that, comprising:

Treatment darkroom;

Carrying platform, is arranged in described treatment darkroom, has the treatment target of nanometric photosensitizer for injection in supporting body;

First laser instrument, is arranged in described treatment darkroom, irradiates described treatment target for Emission Lasers, makes the nanometric photosensitizer in the described treatment target body of injection luminous;

Charge-coupled image sensor CCD, is arranged in described treatment darkroom, for taking described treatment target through optical filter;

Optical filter, is arranged between described treatment target and described CCD, for the light of filtering setting spectrum, makes the light transmission that described nanometric photosensitizer sends;

Turntable, is arranged in described treatment darkroom, connects described second laser, aims at the region of enriching lumination in described treatment target body by rotating the transmit direction adjusting described second laser;

Second laser, is installed on described turntable, irradiates the region of enriching lumination in described treatment target body for Emission Lasers.

2. laser nano optics diagnostic equipment according to claim 1, is characterized in that, also comprise: thermal infrared imager;

Described thermal infrared imager comprises: infrared probe, infrared thermal image technique device and IR image display device;

Described infrared probe, is arranged in described treatment darkroom, for receiving the infrared ray that described treatment target sends;

Infrared thermal image technique device, connects described infrared probe, and the infrared ray for sending according to described treatment target generates corresponding temperature distribution image;

IR image display device, connects described infrared thermal image technique device, shows described temperature distribution image.

3. laser nano optics diagnostic equipment according to claim 1, is characterized in that, also comprise: BOLD contrast;

Described BOLD contrast comprises: monitoring probe, data display equipment;

Described monitoring probe, is arranged in described treatment darkroom, connects described treatment target, for measuring the blood oxygen concentration of described tumor region;

Described data display equipment, connects described monitoring probe, for showing the blood oxygen concentration data that described monitoring probe measurement obtains.

4. laser nano optics diagnostic equipment according to claim 1, is characterized in that, also comprise:

Lighting source, is arranged in described treatment darkroom, for described treatment darkroom lighting.

5. laser nano optics diagnostic equipment according to claim 1, is characterized in that, the laser wavelength range that described first laser instrument is launched is 420-800 nanometer.

6. laser nano optics diagnostic equipment according to claim 1, is characterized in that, the wave-length coverage of the laser that described second laser is launched is 600 ~ 1400 nanometers.