CN204086137U - For device and the analyser of latex turbidimetry - Google Patents
For device and the analyser of latex turbidimetry Download PDFInfo
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- CN204086137U CN204086137U CN201420353480.5U CN201420353480U CN204086137U CN 204086137 U CN204086137 U CN 204086137U CN 201420353480 U CN201420353480 U CN 201420353480U CN 204086137 U CN204086137 U CN 204086137U
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- latex turbidimetry
- diaphragm
- photoelectric detector
- detection diaphragm
- latex
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- Expired - Lifetime
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- 239000004816 latex Substances 0.000 title claims abstract description 38
- 229920000126 latex Polymers 0.000 title claims abstract description 38
- 238000004879 turbidimetry Methods 0.000 title claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 40
- 239000012085 test solution Substances 0.000 claims abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 7
- 102100032752 C-reactive protein Human genes 0.000 claims description 21
- 108010074051 C-Reactive Protein Proteins 0.000 claims description 15
- 210000004369 blood Anatomy 0.000 claims description 15
- 239000008280 blood Substances 0.000 claims description 15
- 238000012360 testing method Methods 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 6
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical group [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 238000013461 design Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 16
- 238000002835 absorbance Methods 0.000 description 7
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 230000004520 agglutination Effects 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 238000007493 shaping process Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108010062271 Acute-Phase Proteins Proteins 0.000 description 2
- 102000011767 Acute-Phase Proteins Human genes 0.000 description 2
- 238000009534 blood test Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 229940028435 intralipid Drugs 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 238000000790 scattering method Methods 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000003219 hemolytic agent Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a kind of device for latex turbidimetry and analyser.A kind of device for latex turbidimetry, be included in the LED light source that light path is arranged in order, aperture, collimation lens, colorimetric pool, first detection diaphragm, and photoelectric detector, wherein, the incident light that described aperture sends for limiting described LED light source, described collimation lens is used for described incident light parallelization, described colorimetric pool is for holding the test solution after sample to be tested and reagent reacting, described incident light forms transmitted light through described colorimetric pool, described first detection diaphragm is used for limiting described transmitted light, described photoelectric detector receives described transmitted light.The above-mentioned device for latex turbidimetry employs the design of the additional diaphragm of LED light source, and eliminate the elements such as the grating in conventional detection devices, small volume, integrated level obtains larger raising.
Description
Technical field
The utility model relates to medical treatment detection device, particularly relates to a kind of device for latex turbidimetry and the analyser for monitoring C reactive protein in whole blood.
Background technology
As everyone knows, the sign of inflammation in human body and degree and process can be observed with the marker of inflammation.Known marker has leukocytic number, erythrocyte sedimentation rate, acute phase protein etc.C reactive protein (CRP) is exactly such class acute phase protein, and its concentration is being subject to not homologous stimulus, and as bacteriological infection, inflammation, can increase fast after operation etc., therefore have important directive significance clinically.
CRP detection method common at present all based on the latex of serum sample than turbid scattering method, detect and CRP detection owing to often needing to carry out routine blood test clinically simultaneously, and routine blood test detection will use whole blood sample, two parts of blood samples will be prepared like this, the blood drawing amount of patient is strengthened, and therefore whole blood CRP detects and arises at the historic moment.Whole blood CRP is also based on latex scattering method, and after the sample haemolysis containing haemocyte, when antigen wherein runs into the latex particle being adsorbed with antibody, antigen-antibody combines and occurs latex agglutination.The size of single latex particle within lambda1-wavelength, light-transmissive.When two or more latex particle agglutination, can hinder light therethrough, transmitted light is reduced, its minimizing degree is directly proportional to the degree of latex agglutination, is also directly proportional to antigen amount, can be obtained the amount of antigen by measurement absorbance or scattered light.
The traditional equipment utilizing spectrophotometer test volume whole blood CRP, comprises Halogen lamp LED, condenser lens, diffraction grating, measuring cell, multiplying arrangement, computing unit and display unit.But this measuring equipment utilization is existing spectrophotometer, volume is comparatively large, is difficult to meet the demand at present to instrument miniaturization.
Utility model content
Based on this, be necessary to provide a kind of small volume and the higher device for latex turbidimetry of integrated level and the analyser for monitoring C reactive protein in whole blood.
A kind of device for latex turbidimetry, be included in the LED light source that light path is arranged in order, aperture, collimation lens, colorimetric pool, first detection diaphragm, and photoelectric detector, wherein, the incident light that described aperture sends for limiting described LED light source, described collimation lens is used for described incident light parallelization, described colorimetric pool is for holding the test solution after sample to be tested and reagent reacting, described incident light forms transmitted light through described colorimetric pool, described first detection diaphragm is used for limiting described transmitted light, described photoelectric detector receives described transmitted light.
Wherein in an embodiment, centered by described LED light source, wavelength is the LED light source of 800 ~ 900nm.
Wherein in an embodiment, the clear aperature of described aperture is 0.2 ~ 0.6mm.
Wherein in an embodiment, described sample to be tested is whole blood, and the determinand in described sample to be tested is C reactive protein.
Wherein in an embodiment, the clear aperature of described first detection diaphragm is 0.5 ~ 0.8mm, and described colorimetric pool and described first distance detected between diaphragm is 2 ~ 4mm.
Wherein in an embodiment, the described device for latex turbidimetry also comprises the second detection diaphragm between described first detection diaphragm and described photoelectric detector, and the clear aperature of described second detection diaphragm is 0.6 ~ 1.0mm.
Wherein in an embodiment, described first detection diaphragm and described second distance detected between diaphragm is 4 ~ 8mm.
Wherein in an embodiment, the distance between described second detection diaphragm and described photoelectric detector is 1 ~ 3mm.
Wherein in an embodiment, described photoelectric detector comprises photoelectric conversion unit and amplifying unit, and described photoelectric conversion unit is used for the light signal of described transmitted light to be converted to electric signal, and described amplifying unit is for amplifying described electric signal.
For monitoring an analyser for C reactive protein in whole blood, comprise the above-mentioned device for latex turbidimetry, and calculate display unit, described calculating display unit is according to the content of the intensity determination C reactive protein of described transmitted light and show testing result.
The above-mentioned device for latex turbidimetry employs the design of the additional diaphragm of LED light source, and eliminate the elements such as the grating in conventional detection devices, small volume, integrated level obtains larger raising, can meet the demand at present to instrument miniaturization.
Accompanying drawing explanation
Fig. 1 is the structural representation of the device for latex turbidimetry of an embodiment;
Fig. 2 is the module map of photoelectric detector in Fig. 1;
Fig. 3 is that the device for latex turbidimetry of an embodiment is at the test scattering coefficient of different sample and the linear relationship data figure of absorbance.
Embodiment
For the ease of understanding the utility model, below with reference to relevant drawings, the utility model is described more fully.Preferred embodiment of the present utility model is given in accompanying drawing.But the utility model can realize in many different forms, is not limited to embodiment described herein.On the contrary, provide the object of these embodiments be make the understanding of disclosure of the present utility model more comprehensively thorough.
It should be noted that, when element is called as " being fixed on " another element, directly can there is element placed in the middle in it on another element or also.When an element is considered to " connection " another element, it can be directly connected to another element or may there is centering elements simultaneously.Term as used herein " vertical ", " level ", "left", "right", " on ", D score and similar statement just for illustrative purposes.
Unless otherwise defined, all technology used herein and scientific terminology are identical with belonging to the implication that those skilled in the art of the present utility model understand usually.The object of the term used in instructions of the present utility model herein just in order to describe specific embodiment, is not intended to be restriction the utility model.Term as used herein " and/or " comprise arbitrary and all combinations of one or more relevant Listed Items.
The device for latex turbidimetry of the application may be used for measuring the determinand in multiple sample to be tested, such as globulin, albumin, special proteins, namely C reactive proteins etc., because the assay method of these determinands all uses latex turbidimetry, when running into as these determinands of antigen the latex particle being adsorbed with corresponding antibody, antigen-antibody combines and occurs latex agglutination, and latex agglutination degree can reflect the concentration of determinand.That C reactive protein in whole blood is described for determinand below.It will be appreciated by those skilled in the art that the device of the application can also be applicable to the detection that other can use the determinand of latex turbidimetry.
Refer to Fig. 1, the device 100 for latex turbidimetry of an embodiment, be included in LED light source 10, aperture 20, collimation lens 30, colorimetric pool 40, first detection diaphragm 50, second detection diaphragm 60 and photoelectric detector 70 that light path is arranged in order.The light that LED light source 10 sends irradiates containing the test solution that C reactive protein and reagent reacting obtain in colorimetric pool 40 after aperture 20 and collimation lens 30 shaping, and the transmitted light of formation is received by photoelectric detector 70, thus obtains the Transmission light signal of test solution.
Preferably, the centre wavelength of LED light source 10 is 800 ~ 900nm.Compare halogen light source, the luminescence efficiency of LED light source is high, and energy consumption is low, small volume.The more important thing is that therefore LED light source Wavelength stabilized do not need to re-use grating pair light and carry out dispersion.
Aperture 20 is arranged on the side of LED light source 10.The effect of aperture 20 is that the incident light restriction sent by LED light source 10 becomes a pointolite, thus is better conducive to follow-up collimation lens 30 and carries out shaping.Preferably, the clear aperature of aperture 20 is 0.2 ~ 0.6mm.
Collimation lens 30 is arranged on the side of aperture 20.The effect of collimation lens 30 is the incident light parallelizations that pointolite can be sent, and is namely shaped as directional light.In the present embodiment, the base portion that collimation lens 30 is made up of the flat board that is circular or square configuration and an aspheric lens combination are formed.It will be appreciated by those skilled in the art that collimation lens can also be other forms, as long as can realize the light shaping that pointolite sends is directional light.
Colorimetric pool 40 is arranged on the side of collimation lens 30.Colorimetric pool 40 is roughly rectangle, and it is generally made up of glass.The parallel incident light carrying out self-focus lens 30 can longitudinally through colorimetric pool 40.Test solution after sample to be tested and reagent reacting is placed in colorimetric pool 40.In the present embodiment, sample to be tested is whole blood, and reagent is hemolytic agent and the emulsion reagent containing antibody.
First detection diaphragm 50 is arranged on the side of colorimetric pool 40.When incident light is through the test solution of colorimetric pool 40, antigen antibody complex in test solution can have certain scattering process to light, therefore use the first detection diaphragm 50 can get rid of this part scattered light, thus make only only have the transmitted light along main optical path could be received by photoelectric detector 70.Find through many experiments, the clear aperature of the first detection diaphragm 50 has considerable influence to testing result.If clear aperature is less, then signal to noise ratio (S/N ratio) will be caused poor; If clear aperature is comparatively large, then the range of linearity of scattering absorbance can be caused to be deteriorated.Preferably, the clear aperature of the first detection diaphragm 50 is 0.5 ~ 0.8mm.Distance between first detection diaphragm 50 and colorimetric pool 40 is 2 ~ 4mm.
Second detection diaphragm 60 is arranged on the side of the first detection diaphragm 50.The effect of the second detection diaphragm 60 limits further the transmitted light through the first detection diaphragm 50, finds, arrange the range of linearity that the second diaphragm can improve scattering absorbance through many experiments.Meanwhile, adopt two to detect diaphragm and compare the length that a detection diaphragm significantly can reduce light path, thus reduce the volume of device further.Preferably, the clear aperature of the second detection diaphragm 60 is 0.6 ~ 1.0mm.Second detection diaphragm 60 and the first distance detected between diaphragm 50 are 4 ~ 8mm.Be appreciated that the second detection diaphragm 60 also can omit.
Photoelectric detector 70 for receiving transmitted light, and determines the content of C reactive protein with this.When incident light is through colorimetric pool 40, in test solution, antigen antibody complex can be absorbed light and be reflected, and transmitted light is reduced.Immune complex is more, and the light of decay is more, and transmitted light is fewer, carrys out the content of corresponding determined antigen with this.Refer to Fig. 2, in the present embodiment, photoelectric detector 70 comprises photoelectric conversion unit 72, amplifying unit 74.Photoelectric conversion unit 72 is for being converted to electric signal by the light signal of transmitted light, and amplifying unit 74 is for amplifying this electric signal.Compare and traditional photomultiplier, what the photoelectric detector 70 of the present embodiment adopted is silicon photoelectric diode, thus can reduce the volume of equipment further, improves integrated level.Preferably, photoelectric detector 70 and the second distance detected between diaphragm 60 are 1 ~ 3mm.
In one embodiment, the utility model also provides a kind of analyser for monitoring C reactive protein in whole blood, and it comprises sample preprocessing device, the above-mentioned device 100 for latex turbidimetry and a calculating display unit.
Sample preprocessing device is used for carrying out pre-service to form test solution to sample to be tested.Calculating display unit can be the computing machine be connected with photoelectric detector 70, and it can receive the electric signal after amplification, and shows testing result according to the content of the intensity determination C reactive protein of transmitted light.
Refer to Fig. 3, be depicted as well-behaved analyzer at the linear relationship data figure testing different sample scatter coefficient and absorbance.Sample to be tested is conventional turbid media analogies Intralipid solution, and based on the Intralipid solution of 10%, (scattering coefficient is for 3.86mm
-1) obtain a series of different scattering coefficient scattering solution by distilled water diluting.As can be seen from Fig. 3, absorbance and scattering coefficient have good linear, and absorbance measuring scope can reach 1.5AU (deviation from linearity ± 5%).
The above embodiment only have expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (10)
1. the device for latex turbidimetry, it is characterized in that, be included in the LED light source that light path is arranged in order, aperture, collimation lens, colorimetric pool, first detection diaphragm, and photoelectric detector, wherein, the incident light that described aperture sends for limiting described LED light source, described collimation lens is used for described incident light parallelization, described colorimetric pool is for holding the test solution after sample to be tested and reagent reacting, described incident light forms transmitted light through described colorimetric pool, described first detection diaphragm is used for limiting described transmitted light, described photoelectric detector receives described transmitted light.
2. the device for latex turbidimetry according to claim 1, is characterized in that, centered by described LED light source, wavelength is the LED light source of 800 ~ 900nm, and/or described photoelectric detector is silicon photoelectric diode.
3. the device for latex turbidimetry according to claim 1, is characterized in that, the clear aperature of described aperture is 0.2 ~ 0.6mm.
4. the device for latex turbidimetry according to claim 1, is characterized in that, described sample to be tested is whole blood, and the determinand in described sample to be tested is C reactive protein.
5. the device for latex turbidimetry according to claim 1, is characterized in that, the clear aperature of described first detection diaphragm is 0.5 ~ 0.8mm, and described colorimetric pool and described first distance detected between diaphragm is 2 ~ 4mm.
6. the device for latex turbidimetry according to claim 1, is characterized in that, also comprises the second detection diaphragm between described first detection diaphragm and described photoelectric detector, and the clear aperature of described second detection diaphragm is 0.6 ~ 1.0mm.
7. the device for latex turbidimetry according to claim 6, is characterized in that, described first detection diaphragm and described second distance detected between diaphragm is 4 ~ 8mm.
8. the device for latex turbidimetry according to claim 6, is characterized in that, the distance between described second detection diaphragm and described photoelectric detector is 1 ~ 3mm.
9. the device for latex turbidimetry according to claim 1, it is characterized in that, described photoelectric detector comprises photoelectric conversion unit and amplifying unit, and described photoelectric conversion unit is used for the light signal of described transmitted light to be converted to electric signal, and described amplifying unit is for amplifying described electric signal.
10. one kind for detecting the analyser of C reactive protein in whole blood, it is characterized in that, comprise as claimed in any one of claims 1-9 wherein for the device of latex turbidimetry, and calculating display unit, described calculating display unit is according to the content of the intensity determination C reactive protein of described transmitted light and show testing result.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420353480.5U CN204086137U (en) | 2014-06-27 | 2014-06-27 | For device and the analyser of latex turbidimetry |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420353480.5U CN204086137U (en) | 2014-06-27 | 2014-06-27 | For device and the analyser of latex turbidimetry |
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| CN204086137U true CN204086137U (en) | 2015-01-07 |
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| CN201420353480.5U Expired - Lifetime CN204086137U (en) | 2014-06-27 | 2014-06-27 | For device and the analyser of latex turbidimetry |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784642A (en) * | 2016-05-23 | 2016-07-20 | 深圳市国赛生物技术有限公司 | Detection device and optical system thereof |
| CN110095418A (en) * | 2019-04-08 | 2019-08-06 | 北京时代新维测控设备有限公司 | A kind of photoelectric colorimetry measuring device |
| CN110178014A (en) * | 2016-11-14 | 2019-08-27 | 美国西门子医学诊断股份有限公司 | Method and apparatus for using patterned illumination characterization sample |
| CN112924418A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Specific protein analysis system |
| CN112924420A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Nephelometry measuring device by scattering method |
| CN112924419A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Light source component and specific protein analysis system |
| CN113588564A (en) * | 2021-05-25 | 2021-11-02 | 上海奥普生物医药股份有限公司 | Diaphragm and optical detection device |
-
2014
- 2014-06-27 CN CN201420353480.5U patent/CN204086137U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784642A (en) * | 2016-05-23 | 2016-07-20 | 深圳市国赛生物技术有限公司 | Detection device and optical system thereof |
| CN110178014A (en) * | 2016-11-14 | 2019-08-27 | 美国西门子医学诊断股份有限公司 | Method and apparatus for using patterned illumination characterization sample |
| CN110095418A (en) * | 2019-04-08 | 2019-08-06 | 北京时代新维测控设备有限公司 | A kind of photoelectric colorimetry measuring device |
| CN112924418A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Specific protein analysis system |
| CN112924420A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Nephelometry measuring device by scattering method |
| CN112924419A (en) * | 2019-12-05 | 2021-06-08 | 深圳迈瑞生物医疗电子股份有限公司 | Light source component and specific protein analysis system |
| CN112924418B (en) * | 2019-12-05 | 2024-08-02 | 深圳迈瑞生物医疗电子股份有限公司 | Specific protein analysis system |
| CN113588564A (en) * | 2021-05-25 | 2021-11-02 | 上海奥普生物医药股份有限公司 | Diaphragm and optical detection device |
| CN113588564B (en) * | 2021-05-25 | 2024-01-30 | 上海奥普生物医药股份有限公司 | Diaphragm and optical detection device |
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